LOCUS       NZ_CP040373              956 bp    DNA     linear   CON 25-JUL-2023
DEFINITION  Enterococcus faecium strain VB3240 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP040373
VERSION     NZ_CP040373.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11633508
            Assembly: GCF_005576735.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Balaji,V., Yamuna,B.D., Karthick,V., Ayyan,N. and Vignesh,N.
  TITLE     Linezolid and vancomycin resistant Enterococcus faecium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 956)
  AUTHORS   Balaji,V., Yamuna,B.D., Karthick,V., Ayyan,N. and Vignesh,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2019) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method :: CANU v. 1.7; UNICYCLER HYBRID v. 0.4.6
            Assembly Name                     :: VB3240
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 136.8x
            Sequencing Technology :: IonTorrent; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/25/2023 04:29:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,746
            CDSs (with protein)               :: 2,746
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 109 of 185
            Pseudo Genes (incomplete)         :: 79 of 185
            Pseudo Genes (internal stop)      :: 28 of 185
            Pseudo Genes (multiple problems)  :: 28 of 185
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040373.1.
            Bacteria and source DNA available from Christian Medical College.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB3240"
                     /isolation_source="Pus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="unnamed5"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Christian Medical College"
     gene            <1..895
                     /locus_tag="FEF08_RS15835"
                     /old_locus_tag="FEF08_15835"
     CDS             <1..895
                     /locus_tag="FEF08_RS15835"
                     /old_locus_tag="FEF08_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_138222790.1"
                     /translation="QGTTTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRL
                     NENQGYLKSVGINGWQLFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFI
                     HDLFLDPHFSRADIACDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASER
                     QVRLYNKKLEQERKKVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPI
                     DIKPIDKIVIDGLIAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADEL
                     KKELDTWLLGLDVTEK"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          14..889
                     /name="rep_cluster_935"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.027"
                     /coverage="85.0"
                     /evalue="0.0"
ORIGIN
        1 gcaaggtacg accacccccc taaaattaaa ttggtcgatt gatcgaatta caattgtcgg
       61 aaaactgaat gataccatag cttattggac gaaaaatgga gttgtttttg tcgatttcga
      121 gcaattaatg cgtttgaacg aaaatcaggg atatttgaaa tcagttggga taaacggctg
      181 gcaattattc gatcaatatg gagaagatat tgcgtatatc gaaatactta aatttcatga
      241 aggaaaaggt cgtattgatt ttaatccgaa taagataggc cagtttttag ctagttccat
      301 gaaaggattc attcatgacc tatttttaga tccgcatttt tcccgggcgg atattgcctg
      361 cgatattgta gatgtgccgg atgactttat tactcagtat cgtattgtag acccgatttc
      421 ttttaagcca atctacggcc gttctggcaa gctagaaaca gcgtactggg gcagtcgtgc
      481 cagtgagcgc caagtgcgac tgtacaacaa aaagctagag caggaacgaa aaaaagtaat
      541 cgtgccaaaa gaaatcgata cgtggtggcg gttagaaatg cagctaagac gtggcaaagc
      601 aacggattgg catgcgatgg ttcaggaaag cttagatagt tttgcaagcc cgcatttttt
      661 gccgattgat ataaaaccta ttgataaaat agttattgat ggtttgatag ctgagccttc
      721 taattggtcg ataattgcta gacatacaaa atataaatat cggaatttgc tgaagcaaga
      781 aagtcaaaat gatgagttaa cgaatcattt acgagagact tttaaggaat ctgcagacga
      841 attaaaaaaa gaattggata cgtggctttt ggggttggat gtgacagaga agtgataccg
      901 aataagaaaa gtcagatgag tgagagtaaa tttacgtcaa tgtttagtga cgtgga
//
