LOCUS       NZ_CP040372             1122 bp    DNA     linear   CON 25-JUL-2023
DEFINITION  Enterococcus faecium strain VB3240 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP040372
VERSION     NZ_CP040372.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11633508
            Assembly: GCF_005576735.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Balaji,V., Yamuna,B.D., Karthick,V., Ayyan,N. and Vignesh,N.
  TITLE     Linezolid and vancomycin resistant Enterococcus faecium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Balaji,V., Yamuna,B.D., Karthick,V., Ayyan,N. and Vignesh,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2019) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method :: CANU v. 1.7; UNICYCLER HYBRID v. 0.4.6
            Assembly Name                     :: VB3240
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 136.8x
            Sequencing Technology :: IonTorrent; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/25/2023 04:29:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,746
            CDSs (with protein)               :: 2,746
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 109 of 185
            Pseudo Genes (incomplete)         :: 79 of 185
            Pseudo Genes (internal stop)      :: 28 of 185
            Pseudo Genes (multiple problems)  :: 28 of 185
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040372.1.
            Bacteria and source DNA available from Christian Medical College.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB3240"
                     /isolation_source="Pus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="unnamed4"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Christian Medical College"
     gene            complement(228..>1122)
                     /locus_tag="FEF08_RS15830"
                     /old_locus_tag="FEF08_15830"
     CDS             complement(228..>1122)
                     /locus_tag="FEF08_RS15830"
                     /old_locus_tag="FEF08_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_138222788.1"
                     /translation="DDKVHPLKLSWSIDRITIVGKLNDTIAYWTQNGVVFVDFEQLMRL
                     NENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKFHPDKGRIDFNPNKIGEFLKGSMKQFI
                     HDLFLDAHFSRADIACDILGVPDDFITQYRIVDPVSFKPIYGRSGKLETAYWGSRASER
                     QVRMYNKKLEQERKKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYLPG
                     DVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESANDL
                     KIELDTWLLGLDVTEK"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          complement(229..995)
                     /name="rep_cluster_1968"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat tccaaacgct ctccgagggt
       61 tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag cgtatcacgc tatcaactcc
      121 ttttacgctt tattttgctt cacgcaaaat aatcgcttat cgtaaattca cgtcattaaa
      181 tattgacgta aatttactct cactcatctg acttttctta ttcggtatca cttctctgtc
      241 acgtccaaac ccaaaagcca cgtgtctaat tctattttca agtcgtttgc agattcctta
      301 aacgtctctc gtaaatgatt cgtcaactca tcgttttggc tttcttcttt caacaatttg
      361 cgcaatctat atttcaaatt tctgctaata tacgcccact ctgaatgatc ttgattcagc
      421 ccttttatca taattctatc aacaggtttt acatctccag gcaagtaatg aggacttgca
      481 aagctatcta aactttcccg aaccatcgca tgccaatctg ttgctttgcc acgtcttagc
      541 tgcatttcta tacgccacca tgtttcgatt tcttttggta caatctgttt ttttcgttcc
      601 tgttcaagct ttttgttgta catccgcact tgtcgctcgc tggcacgact accccagtac
      661 gctgtttcta atttgccaga acggccataa attggcttaa aagacacagg gtcaacgatt
      721 cgatactggg taataaaatc atcaggaaca ccgagaatat cgcaagcaat atctgcacgt
      781 gagaaatgcg catccagaaa caaatcatga atgaattgtt tcatcgaccc ctttaaaaac
      841 tcgccaattt tatttggatt aaaatcaata cgacctttgt cagggtgaaa ttttaacatt
      901 tcgatatagg cgatatcttc tccatattgg tcaaataatt gccaaccatt tgtaccaacc
      961 gacttcaaat acccttgatt ttcattcaaa cgcattaatt gctcaaaatc gacaaacaca
     1021 actccatttt gcgtccaata agctatggta tcattcagtt ttccgacgat tgtaattcga
     1081 tcgattgacc aacttaattt tagggggtgt accttatcgt ca
//
