LOCUS       NZ_CP040371             1718 bp    DNA     linear   CON 25-JUL-2023
DEFINITION  Enterococcus faecium strain VB3240 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP040371
VERSION     NZ_CP040371.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11633508
            Assembly: GCF_005576735.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Balaji,V., Yamuna,B.D., Karthick,V., Ayyan,N. and Vignesh,N.
  TITLE     Linezolid and vancomycin resistant Enterococcus faecium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Balaji,V., Yamuna,B.D., Karthick,V., Ayyan,N. and Vignesh,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2019) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method :: CANU v. 1.7; UNICYCLER HYBRID v. 0.4.6
            Assembly Name                     :: VB3240
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 136.8x
            Sequencing Technology :: IonTorrent; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/25/2023 04:29:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,022
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,746
            CDSs (with protein)               :: 2,746
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 109 of 185
            Pseudo Genes (incomplete)         :: 79 of 185
            Pseudo Genes (internal stop)      :: 28 of 185
            Pseudo Genes (multiple problems)  :: 28 of 185
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040371.1.
            Bacteria and source DNA available from Christian Medical College.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VB3240"
                     /isolation_source="Pus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="unnamed3"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Christian Medical College"
     gene            692..1657
                     /locus_tag="FEF08_RS15825"
                     /old_locus_tag="FEF08_15825"
     CDS             692..1657
                     /locus_tag="FEF08_RS15825"
                     /old_locus_tag="FEF08_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_125276130.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNLNTPLKLQWSIDRITIVGK
                     LKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKWQD
                     GKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKVVPKEVETWWRLEMQLRRG
                     KATDWHNMVQESLNSFASLHFLPIDTTPTDRIMLAGLMSDQNFWSLLSRNSKYKYRELL
                     KEESQNDELTNHLRETFAESADNLKQELDTWLLGLDVTEK"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          771..1604
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.141"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 cgaaggaggg gtttaacttg gggtttagta tttcgttcaa aaagggaaca aaaaatacaa
       61 gcatttctca taataatcgt gagttaactg atagccaaaa aattaatagt ttgtctggta
      121 gaaaataatt agcagattta caagtgggaa ataaaaagct gaaagaagaa aattgcattt
      181 caaactacat tttctacaat aactctaaaa tggcatattt tgcgttctga gcggttttag
      241 cgtttatttc gtttagttat cggcacaatc ggtgaaacac gttagaggag cgaaaatgga
      301 gcctgtaagg cgactggtag cggaacggtc gtgttagatt gtgtagccga tagctgaacg
      361 aaataaatgc tggcagcgcc attctatttc ggcaggagga ggctcaaggg gttcggggaa
      421 tgaaattccc tgaggtcttt ttattttttc tagaggagtg ctataatcaa agaaaatgag
      481 aaaaaagagt aaaaaaatag tcgagcagtg ttgacgcact ctcgaccttt acggaaacta
      541 atcacactag ctccgtaact tcttaatcat tatacgaata tttttagatt tcttcaagac
      601 tagagatttt ttggtaatga atttttaaga gttacgtgaa cttttgaaaa ttctttccag
      661 aagtctcttt ttctttgagg aggaacaaaa tttgagtaaa aatggcataa tcgtaaatga
      721 gtgggaaaat ttaacagccc tatctaacag gcggttaaat gttcaaaatt tgaacacccc
      781 cctaaaatta cagtggtcaa ttgaccgaat tactatcgtt ggaaaactga aggaaaatat
      841 ttattatcac actcaaaatg atgtgttgat tttagatttt gagcaattaa tgcgggtgaa
      901 tgaaggcaat ggctatttaa aagcggttgg gaataacggc tggcaattat tagaccaata
      961 cgaagaaaat attgcttata ttgagatttt gaaatggcaa gatggcaaag ggcggattga
     1021 ttttaacccg aataagattg gtcatttttt agctagttca atgaaaaatt tcattcatga
     1081 tttatttcta gaaccacatt tctcacgtgc ggatattgct tgcgatatta tcgatgtgcc
     1141 tgatgagttt attacacaat atcgagtggt tgatcctgtt tctttcaagc caatttatgg
     1201 gcggtctggg aagctggaaa cagcttattg gggcagtcgt gccagcgaac gacaaattcg
     1261 gctatacaat aaaaaattgg aacaagaaac caaacgaaaa gttgtaccaa aggaagttga
     1321 gacttggtgg cggttagaaa tgcaactacg gcgtggcaag gcaaccgatt ggcataatat
     1381 ggttcaagaa agtttgaata gttttgcgag cctgcatttt ttgccgattg atacaacacc
     1441 tacagataga ataatgctcg ctggtttaat gtctgatcaa aatttctggt ctcttttgtc
     1501 gaggaattca aaatataaat atcgggaatt gctgaaagaa gaaagtcaaa atgatgaatt
     1561 gacgaatcat ttgcgagaaa cgtttgcaga atcggcagac aatttgaaac aagaattaga
     1621 cacgtggctt ttgggtttgg acgtgacaga gaagtgatac cgaataagaa aagtcagatg
     1681 agtgagagta aatttacgtc aatgtttaat gacgtgag
//
