LOCUS       NZ_CP040271             4087 bp    DNA     circular CON 21-JUN-2024
DEFINITION  Escherichia coli strain 1500 plasmid pEc1500_2, complete sequence.
ACCESSION   NZ_CP040271
VERSION     NZ_CP040271.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10784802
            Assembly: GCF_008642415.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Grevskott,D.H., Salva-Serra,F., Lunestad,B.T., Moore,E.R.B. and
            Marathe,N.P.
  TITLE     Complete sequencing of ESBL-producing Escherichia coli isolated from
            the environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Grevskott,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2019) Contaminants and Biohazards, Institute of
            Marine Research, Nordnesboder 3, Bergen 5005, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.0.
            Assembly Name                     :: Ecol_1500
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008642415.1-RS_2024_06_21
            Annotation Date                   :: 06/21/2024 03:07:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,679
            CDSs (total)                      :: 4,563
            Genes (coding)                    :: 4,454
            CDSs (with protein)               :: 4,454
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 49 of 109
            Pseudo Genes (incomplete)         :: 61 of 109
            Pseudo Genes (internal stop)      :: 21 of 109
            Pseudo Genes (multiple problems)  :: 22 of 109
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040271.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="1500"
                     /isolation_source="marine environment"
                     /host="Bivalve mollusk"
                     /db_xref="taxon:562"
                     /plasmid="pEc1500_2"
                     /geo_loc_name="Norway"
                     /collection_date="2014-10/2015-11"
     gene            join(2478..4087,1..550)
                     /gene="mobQ"
                     /locus_tag="EST53_RS23485"
                     /old_locus_tag="EST53_23485"
     CDS             join(2478..4087,1..550)
                     /gene="mobQ"
                     /locus_tag="EST53_RS23485"
                     /old_locus_tag="EST53_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029344.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALVNAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(806..1513)
                     /locus_tag="EST53_RS23490"
                     /old_locus_tag="EST53_23490"
     CDS             complement(806..1513)
                     /locus_tag="EST53_RS23490"
                     /old_locus_tag="EST53_23490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001296720.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(2045..2299)
                     /gene="mobC"
                     /locus_tag="EST53_RS23500"
                     /old_locus_tag="EST53_23500"
     CDS             complement(2045..2299)
                     /gene="mobC"
                     /locus_tag="EST53_RS23500"
                     /old_locus_tag="EST53_23500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2301..2477)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2478..2836
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.722"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(808..1513)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.859"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3..547
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.11"
                     /coverage="99.0"
                     /evalue="1.02e-90"
     Typing          2392..4085
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.646"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ccgccagccg aagacatggc cggagtcttt ctgtcttgga gaagaccgcc agccagccat
       61 gccatcactg gcagcaagga aggtggattt tacgcaggga cgtctgctac cgacgctgat
      121 gccggtaatg agttctgtcg accgggaaac ccgccagctt cagcttttgc tggtcatggg
      181 cgtggatgac tctctgggcg gtgttgtccg gcttaatggt acgctgtatc ccgcgtttgc
      241 cgttccctca gccgataaca gccagctggt catcagtgca ctgaccgata aggggttacg
      301 gtatgcaggg tatggtgttg ccgtaaatca cgatgcagac agccacatat cacctgcgcc
      361 ggaacttatg gagtttcatc tgaaaacccg tgaggcaccg ctttttgctg ccgtcaacac
      421 accagaaaaa cagccggatc atctgttccg ctcgctgggt tttaatcgta cctgggacga
      481 atggcggcgc gaggaagatg cacgaacaca cgccaccgag aggagacatg atcgcggttg
      541 gtcacagtga atgtgaacaa tattttgttt tgtttaacat cgtgttatgt tcgtcgatgt
      601 tcttcgatgt tcatcgacag ggatgaatgc aagaagcaca cacataatgc aagcatagtt
      661 ttgtttttcc gcccccaaaa aatgggggtt cttttttcgc tgcattagag cgcttttgac
      721 gcaatttttc acttcaatag taccttcgca ctactaacac aaaaaaaacg cagcagggag
      781 cgttttaggt gccttaaaat cgattttaga tcgtcatctt gatttgtttg tcttccttga
      841 agcgaaaatg cagggctaca accgtgcgcc cctttttgat cgtctcaacc gttacagcca
      901 ggtcgctttt tttgttcagt tcgtcaacac agggttttat tagatccctg tttaacgttt
      961 tgaaatcttt atatttacca tcaagcatca gtgatgattt aaaatcatcg agggcgataa
     1021 tacgttcccc agtactgcgg aattgctgga gaatttcgta tattcttatg ctgtaacttc
     1081 ttgataaatt agaaatattt ttaatgacga ctctggtgaa ttgccccttc aactgtgtca
     1141 gataaggcat taccgcgtca gaaaaagtga tttgtgcttt cccctctccc ctcgcgtact
     1201 gtgcccgata ctgaatccag cggaactcct cgcgcttctc gtcgtccttg agaatgatcg
     1261 acctgtccca caaacgatcg atcgcttcct gtagctgaac ctcggcattt ttccgcccca
     1321 tatcagggaa tgaatcaaaa tattccgcag ctgtaatggt cattgtcttt tgcaattcag
     1381 gtgaagctac gtcagtaccg gagttaagtc ggcttatgca gagcaataag aaacgctgtt
     1441 cttgtaaagt tagcttgtaa ctggcctcaa ccagcgcatt ggacttatag gctatcagag
     1501 ggtttgatgt cattttaacc accaggccaa aaatgaggtg aaataaaaac ctatcacctt
     1561 tagcattggt gagcaattat attttcaccc cccattttcc tgtgaatatt accttaaatc
     1621 tcctcacctt taccttaaat tacctcaccc aagcacctta aattatatca cctttacctt
     1681 aaatcatatc acctatagcg ttgtaacatt ttgttttcta taaggaattt tggcgctaaa
     1741 acaagtatta aaaacaagat cttaaaaaca agatctaaaa acttaacgac gagcctgtgg
     1801 ataacttgga gtactgaaaa caaaaaacca ataaaaacaa tgcgttgcaa aaaagcagaa
     1861 ttgcaagttc cgaatacacc ccctccaacc ctgtggaaat ccagtattca tgcgggttca
     1921 gcgcatatca cctcccgaaa aaccgtgcgt atgtgctcaa tcaggcgacg gattcacttc
     1981 cgcttcctcg cccactcgct cgctaacgct cggtcgctgg gactgcggcg agcggacgat
     2041 acaatcactg tcctggcagc gcttccagct cgttaatcag cggcagtatg cgcgtcacat
     2101 cagcctgtcg tgtcacctct gccttcagga actggagtaa ccaggcacgc aactgcacct
     2161 cctccggctt tctgaccttt gccagcaatg ctgcacccac aatgaccttc tgcgctgtgt
     2221 ccagactcct gctcttcgcc ttcagccgct gtaatctggc ctcagcatca gcaatcttct
     2281 gttcgagtgt tctgctcatg atgtaactcc gtgcgcgatg aaaaatcgca ttttagcgcg
     2341 tcactggtag tttaaaaact gagctggcat aatgcacgga aaatcacgaa gtgcgcactt
     2401 atacaatctc cactccgttt cgattgtgtg cgccctgcgg ggctgaaaga aaaccacaaa
     2461 aaggcattac ggcagaaatg gcgatttatc atctcagcat gaaaatcatc tccagaaaca
     2521 gcggctacag cgccgttgct tctgctgcct atcgttccgg ttcactgatg cttgatgagc
     2581 gtaccggact gactcacgac tacacccgca aaagcggtgt ggctgaagct gtgattctga
     2641 ctcccgcgac agcccctgcc tggtgtacga accgtgccga actctggaat gccgttgaga
     2701 aggccgaacg ccggaagaac tcacaacttg cgagggaaat tgaactcgcc atcccccgcg
     2761 aactaccgca ggatgccgca cgggaaaccg ttctcgcctt tgtcagagaa aactttgtca
     2821 gtcagggcat gattgctgat gtggcgttcc atcacatgga taaaacaaat ccccatgcgc
     2881 acatcatgct gaccacgaga gctgtcggac ctgctggatt cggtggaaag gtcagggact
     2941 ggaacgaccg gacacatgcc gagacgtggc gtgcatcatg ggctgaccat gcgaacaggg
     3001 cactggtgaa cgccggttat caggaagaga tagaccatcg ttcatacgag cgacaggggc
     3061 ttgagaaaac gcctggtatt cacctcggaa agtctgcctg tgcgatggaa acacgcggta
     3121 tcgaaacaga acgcggcgaa cagaatcgtc tgattaacag acttaacctg gaaatacaga
     3181 tttcccgtac cgaactcaga aacagcgggc tgacagagcc tgcccgccgt gtggctgacc
     3241 ttctgaatat cgtgcttcct gacaacgcaa cttcgtacac actacgtgac atcatcgaag
     3301 cgttgccgaa ggacagtaat gcggcatgga aactcaccag taatgccatg tcgatgatgg
     3361 cagatatgca ggcgacgcgc agacgctggg aagagctgaa caccgaacga aaggaagcga
     3421 tgagccatgc tgccagcctg aaaaaatcat cgccgttttc gtccggtttt tcccgtattc
     3481 ccctgatgca atgggcggca ccggaatacc gtagcgaaca gggaaaaatc cagtcgctga
     3541 gccagcaaat ggaaaaattg cgacagcatt accggaaggt ggagaaacag gacattcccg
     3601 cgagtcagac ggcatttgaa aaacagtggc agcagtgggg ggcatccggc cttgcagctc
     3661 tcagagtcca gttgcttaaa cgtgaagagg aattgcgccg gaaggagcag gaggatacag
     3721 aacgtagtcg cgcggagcga cgagcggaac agctcaggaa aaatgacaat gccgttatgg
     3781 attcaggggt actgagcgca gtcgttacgg ggtatggcga ggcccccatg cctggtaacg
     3841 gggaaatgac ctgttacctt ctgcttcaca accggaacgg agaatacacg ctctggggga
     3901 acgagctgga gaaataccgc accagaatct ttgagcgtgt tgatttaatg cgtgacagga
     3961 gtggttatat ctgtgaccga agcgaaatcg gtcaacatcc accactgcaa cgtgtttatt
     4021 cctctgcgac ctttgagcaa cttctgacgc aggtttgcca gacatggccg caatataccc
     4081 gcaatct
//
