LOCUS       NZ_CP040125            10060 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain LSH-KPN148 plasmid pLSH-KPN148-3,
            complete sequence.
ACCESSION   NZ_CP040125
VERSION     NZ_CP040125.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11602135
            Assembly: GCF_009914235.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Huang,J.
  TITLE     Genome sequence of Klebsiella pneumoniae strain LSH-KPN148
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Huang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2019) Clinical Laboratory, Lishui Hospital,
            Zhejiang University School of Medicine, No.289 Kuocang Road, Lishui,
            Zhejiang 323000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.11.1; HGAP v. 2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 04:49:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,751
            CDSs (total)                      :: 5,630
            Genes (coding)                    :: 5,462
            CDSs (with protein)               :: 5,462
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 66 of 168
            Pseudo Genes (incomplete)         :: 104 of 168
            Pseudo Genes (internal stop)      :: 18 of 168
            Pseudo Genes (multiple problems)  :: 20 of 168
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040125.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="LSH-KPN148"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pLSH-KPN148-3"
                     /geo_loc_name="China"
                     /collection_date="2015-09-01"
                     /collected_by="Hui Ding"
     gene            join(9859..10060,1..1763)
                     /locus_tag="FDY04_RS28140"
     CDS             join(9859..10060,1..1763)
                     /locus_tag="FDY04_RS28140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            1763..2494
                     /gene="mobC"
                     /locus_tag="FDY04_RS28145"
     CDS             1763..2494
                     /gene="mobC"
                     /locus_tag="FDY04_RS28145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            2501..3031
                     /locus_tag="FDY04_RS28150"
     CDS             2501..3031
                     /locus_tag="FDY04_RS28150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3058..3237)
                     /locus_tag="FDY04_RS28155"
     CDS             complement(3058..3237)
                     /locus_tag="FDY04_RS28155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3263..3691)
                     /locus_tag="FDY04_RS28160"
     CDS             complement(3263..3691)
                     /locus_tag="FDY04_RS28160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(3855..4169)
                     /locus_tag="FDY04_RS28165"
     CDS             complement(3855..4169)
                     /locus_tag="FDY04_RS28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4157..4435)
                     /locus_tag="FDY04_RS28170"
     CDS             complement(4157..4435)
                     /locus_tag="FDY04_RS28170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(4610..4975)
                     /locus_tag="FDY04_RS29130"
     CDS             complement(4610..4975)
                     /locus_tag="FDY04_RS29130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(4972..5343)
                     /gene="tolA"
                     /locus_tag="FDY04_RS28180"
     CDS             complement(4972..5343)
                     /gene="tolA"
                     /locus_tag="FDY04_RS28180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(5617..5862)
                     /locus_tag="FDY04_RS28185"
     CDS             complement(5617..5862)
                     /locus_tag="FDY04_RS28185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6189..7874
                     /locus_tag="FDY04_RS28190"
     CDS             6189..7874
                     /locus_tag="FDY04_RS28190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7884..8141
                     /locus_tag="FDY04_RS28195"
     CDS             7884..8141
                     /locus_tag="FDY04_RS28195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            8226..8375
                     /locus_tag="FDY04_RS28200"
     CDS             8226..8375
                     /locus_tag="FDY04_RS28200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1189..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(8479..9057)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1764..2491
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 atgccgatcg ctataagcgc gataataacc ggactgtgcc attttcggtg ctggcctcgc
       61 tggattcacc cgggcggatg gtcagggcac ggcgacatgt ctggaactac gcgtactggc
      121 tgaccggcgc tgccggtctg gcccttctgg gggggctggt tgtcgccgtg tttatcgaga
      181 gctggggaga aaccgtcctg cgcccggacc tctatgcgaa cgcagattat tacctgctgc
      241 agccggctaa cctgttcggg atagcctggt gtgccggagg gcttgtcgcc ggcgtccttc
      301 tggcgcggct gctggcgttc ctgctgcttg atggtcattt tgcggccgct gacgaggcgg
      361 tgaacgcgaa actgaatcag gagagccggc gcagcagcca gcggaccgga gaaatgacag
      421 acgtccgcca cctgcatttc ggggagccgg taccggttaa cgcgctggcc gatttttcca
      481 cggagcaggc ccggaaacag caggcggtgt ttctgggtaa ggatgagcag ggccagccgg
      541 tgctggtccc tcgtgatacc tggcgcaaga ccaatattca gatcctcggc ctgcccggga
      601 gcggcaagag cgcgatggcc accaacgcgc tcattcgctg cgtacgcgat ttcggggacg
      661 ctgtggtgta cttcgacccc aaaggggacg cgtgggcgcc gcacgtcttc cgggcgcact
      721 gcccggactt caccctgctg gatctgcggc cggggaaacc ggcgcagctg aatctgttcc
      781 gcgatctcga ccagtacgcc ctgaagaatc tgctggttgc cgggtttaac ctgagtgaga
      841 ccggcgacgt ggcggaccac taccgcatca gcgagcagaa agcggcgaag ctgattgcgg
      901 agcagttccc gcagggggca aacattcagc aggtgctggc ggcggcgtac gcgttgccgg
      961 aggccctgaa aaaggacgtg aaggggctga tcaccaagct ggagaacgtg gccgacctga
     1021 gcgtcctgca gacggacagc ggtatcgatg tggcggggat aatcaacggc ggcggctgcc
     1081 tgtacgtcat cggctcgatg gacgatgagg cggtgatccg ggtgcagaag atgctgtttg
     1141 cccgctgcgc gcaaatcatc attgcgcggg atgagttccg gaggtggccg cacgcgagca
     1201 tcatgctcga tgagattaaa tatctgctgt cgaagtacgt gctgaacgcg ctggggacgc
     1261 tgcgcagccg ggactgcaac ctgctgctcg cgcaccagtc gctgggcgac ttcggtcagt
     1321 gcgggcagga tctgcccgct gattttgtga aaaccacggt actggataac acgccgatcc
     1381 gctggttcta tcgcgcggcc agccaggagt ccgcgcagtg ggcggcgggc cagaccgggg
     1441 agatccgggt ggacgtggag cgccgccggg ccagccggga ggcggggaac gtggagcaca
     1501 tcagcggcga cagcttcatc cagaaggagg cgcggccgct gtttgacgtg aacaccctgc
     1561 aacacctgcc tgacgggttc gcggtgatga ccgggctggg cgtggcgcgg ctggcgttca
     1621 gcagcccgct gcgggttgag cgccgggaaa tttcgctgaa aaccttcccg gtgctggcga
     1681 aaacggaccc gctggcggag tacctgcctc cggccgacgg cggcggacca aaagcggatg
     1741 atgattttgc ggggctttac tgatgacact ggagcagtac aacgtcagcc acgcgaagcg
     1801 gcaggcgcgg aacgcggaga aaacacggct gaccctgcgc tggctgcggg aagagctgtg
     1861 ttccacggcg gagctggttg cgcgacggct ggggattgcg gcggtacagc cggtttaccg
     1921 cttcctggac agtctggtgg cgaaagggct gctggtccgg gcgaaatacc cggttgacgg
     1981 ccgtcaggtc agcgtgtggg ggctgacgcc gcacggggtg gcgttctcgt tcgacgagga
     2041 cgagccgctg acggacgtta ttccgtttca gccgtcaagg gtgtcggcgg cgcagctgcc
     2101 gcaccggctg gccgtgcagt ccctgcgcct ctccatggag ggccgggggg ccaccggctg
     2161 gcggtacctg caccgtatgg ccctgaaagg gatgaaagtg ccggacgcgc tggcggagct
     2221 ggacggcaga acggtggcat ttgaggtaga gcgcaccgtc aagagccgca ggcggtatca
     2281 ggaagtggtg tccgggtatc tgttcaaccg ccgggcgaac ggcatcgatg aaatctggta
     2341 catctgccct gaccggccga cgcttgtccg ggtacagcgg gcgattctgg cggtggatga
     2401 aatcgtgaat ccacagaccg gcgaggcccg caaaacggtg gagctggacc gggagaggct
     2461 gttcgcgtgc tttaaattta tgacaacgga gtgagggatt atggcgctgt acatggtatt
     2521 tattgaggac gtggaagaca aagacggcga gcgctgcatt atgccaacca gcgagcatct
     2581 ggaagacatg gtggtgctcg cctggcagac cggggcgtat gttttctgtt ttgtcgaccg
     2641 ggcccagcat ctggcgatga tgaaatccat cgggatgtat gacgagcggc tcaacagcga
     2701 cgaaaaagcc acgtactttt tcaccggtga aaatccgcaa tgggaacggg acaaacccac
     2761 aggaatgaag ctgtgtgatt acctctatcg aaaggtggag cggttcgcca tcgagaacgg
     2821 tgtgcaggtg gttgatgccg ggaagtgctc accggagcag attaacgcga tgattgaggt
     2881 ggtcagcgag cggcacgggg aaagcgcggt gaacgagaag ctgaaagcgg agctgatgcg
     2941 ggaatataac agccgctatt ttaacgtgcc ggtgcctgaa ctgccggagg acaaacccgc
     3001 ccgccggtgg tggccgttcg gcaaaaaata acgccagcac cggcatggcc ggtgctgtta
     3061 ttcttccccg agcttatccc gcatcacctg ccagagtgtt tccgcatcct cgtgaagccg
     3121 ttcgcacatg tcggccgggg tatccatgtc cagctcgttc agcttttcca gcaacagcag
     3181 cgactgatcc ttaatgaatt tagccatatt gagggcagtt ttttcctgct tcgtcatgtt
     3241 ctccattccc ttcggtaagt tctcagctaa accagccgga ccagaagcgt tttttcttct
     3301 gtgtcagttc tgttgtcaat ctgtccactt cggcctggag tttccggcgc tcatcatcgt
     3361 gctggcggcg ttcttcttcc cgggatgttt tatcttccag catcagggtg acagcctgcc
     3421 ggagatcggc cagttccttt tgcattgcca gaagctgttc ctgtgggaaa tttttttgtg
     3481 tattttcacg gtgtgaaatg ccgggaattt gtttctcagg gatattcaca tttccgtata
     3541 cacggatcag ttctgacaca tccacgacag ggttgttttt cccgtcacgt gacacggtaa
     3601 cctttccctg ttttacatga ttgtatagcg ttcttctggt aatgcccgcg gcttgtgctg
     3661 cctgtgaaag gttgagcaat gttttcgcca ctgtataccc ctgattttat cgggtgtgaa
     3721 aggtgtgtaa tttctcacgg taaatgtatc gctgaatttt acccgttttt ccgttcagga
     3781 tgtggttttc tgacgcgctg cgcttgtcaa accggcgacg gccacgcaat gaattgcgcc
     3841 cccgtcatcg gttattagag gctgtctgcc cccggatacc cggattcttt ctgatgcctg
     3901 acggtgacga tcacaatctg gtccatttcc cggtcgtggc ggtagagcat cacatagcca
     3961 ctgtcgccaa acccgatcac cagttcctta tattccagcg gcagaaacgg caccgggcgg
     4021 ccaatgtctg gcaatgtttt cagttgctgg atagcccgga cgatcacctc acctcctttt
     4081 ctggctgcca gccgattttt ggttttgaga aagtcctgaa ggcgttgtaa atcgcgctgt
     4141 gccagcgcgg aaatcgttac ctgtggcatg gcggagcatc ctgctcgttc tcagttcccc
     4201 aggtacttat ccaggcttcc gcttcttccg cagtaaggtg cagacccgtt tcctgatagt
     4261 gctgccatgc ggcctgtccg tcccgcaggt actggctgcg cttctcttcc cggtcgatgt
     4321 attcggtgat tgcctccagc attagcgcgt gcgctgagcg gtgccggtca tcggccagcg
     4381 ttttcagccg gtccttaagt tcttcatcga gcctgatgga tgttgctgat gacataaaag
     4441 cccctttgta gtcatatgta ttacacatga ctactttaat ccgtggcggc gctatttgcc
     4501 agaaatacgg cttttgctgc ccatttttca gcttcgcttc cactaatcag ggctgctaca
     4561 ggctcactca tcatccagga ctattgcagc ctgcgggtta aaggacctgt tactggcggc
     4621 aaccatcatc attgagcttt tgggtggtga ctggtcgcgg atcctgtaag ccgtcatttt
     4681 tcagactgaa ttcaaaattt ataacctgtc gcattgccgg gtgccctgcg ggatatagcc
     4741 agtgcctcat tgccgtcagc gtttcccgaa aaaaaaggtg acagccggta agcgacacgt
     4801 tgtccactcg tccatcgtcc agaatatcaa acgtggcggt cacctgacct tcgatgcgtt
     4861 ttgctgcggc ctgtaccggg taggttggct gcagtgtggt tacgggtttt agtggtatcg
     4921 catgtaccgg agtgaggaac atggccgcca gcactgtcag agtaaaagct ttcatggtct
     4981 gaaatccaga ggggcgtttt tgaatatttg ccaggtattt tcatccggtg ccggcggaat
     5041 tttggcgtgg gtaacggctg atattgctgc tttacagagt ttcggatctc ctccttcagc
     5101 aatggcgctg ttcagagtgc catcacgttg tatggatatc cgcacggtgc acatttttcc
     5161 cgcataggcc tctggttcaa acatctgcat ttcaacagcg gtcttgatgg ccgcagcgta
     5221 gctactgatt ttagatgact tatccagagt ataaattttc ggcggctggg tggtggaatg
     5281 cttacaggcc gtcaggagta aggggctgca caatgccagc aggataaagg gtattaaacg
     5341 catcgggttg atgacctgtc agtaaaaaat gttacatgta tatttgtcac tctttacggc
     5401 cacgttcatt tacataaaca cggcttatcg tatcgttttt acaccaaacg gcccgccatg
     5461 cagttgcgtg cataggcatg cagcgggtta caatttaccg ggcgcgtttc cggcggtttt
     5521 ccgggtggtt tgttgcctgt tttatcccgt agccgccgga aacgccctca gaccgtctga
     5581 gcggtgcggg caatgcgacg ttatggtaaa taccccttac cggcggcgct gtggctgcca
     5641 ggtgaaggtg gtttccgtag cctcgtattt ctcccgacgc atttcctgcg cccgcctggc
     5701 ttcacgttcg tcctgaatgg cacgaagcac cctttcccgc acctgttgtt ttaccgcctt
     5761 tggcagcgtt ttgttccctt tcaggcccat ggccagtgcc tgagcgacaa aggcgtccgc
     5821 caggcgcttt tcttcagcgg gagtcagttt caggtgcgtc atagtggcct ccgggcgtct
     5881 caggtttccc gaacagttta accgcaggtc ctctcctcat gccagttagc tttcagatgt
     5941 gtctatacca tctgaaatgt aagtgtgatg aatgtcttaa ataataaaat ttgttgccat
     6001 gagaaatttt cgggaaaaga cgcgatctgc ttacaggtgg ctccgggcgc ttttttgtgc
     6061 ctgaagatga gaactaatct catatattga cacctgaaaa cgggaggagt aaggtaataa
     6121 ttatactgtg tatatgtaca gtatgttggc tttaatttat tgttttaaat gtcaaagagg
     6181 aaaacggtat gagtggtgga gacggtcgag gtccgggtaa ttcaggtctg ggacataatg
     6241 gtggtcaggc cagtgggaat gtgaacggta cgtctggtaa aggtggccct tcatcaggtg
     6301 gtggtacgga tccaaacagc gggccgggct ggggtacgac gcatacgccg aatggagata
     6361 ttcataacta taatccgggg gagtttggta acggcgggag taaacccggt ggtaatggtg
     6421 gtaacagcgg caatcatagc ggtagctctg gtggcggaca gtcttcggcc actgcgatgg
     6481 ccttcggtct gcctgccctg gctactccgg gggctgaagg actggcttta tcagtttccg
     6541 gcgatgcgtt gtcggccgcc gttgctgatg tgctggctgc cctgaagggg ccgtttaagt
     6601 ttggtctgtg ggggattgcg atctacggtg tgctgccttc tgagattgca aaagatgatc
     6661 cgaaaatgat gtcaaaaatt atgacgtcat taccggccga tgcggtgacg gagactccgg
     6721 caagtacttt accactggac caggcgacgg ttcgtgtcag acaacgggtt gtggatgtgg
     6781 tgaaggatga gcggcagcat attgcggttg tcgcaggtcg gccaatgagt gtccctgtgg
     6841 tggatgcgaa accgacaaaa cgtccggggg tattcagtgt gtcgattccg ggtctcccgt
     6901 ctctgcaggt gagcgtaccg aaaggtgttc cgacagcgaa agccccgcca aaaggcattg
     6961 ttgctgaaaa aggtgattca cgtccggctg gttttacagc cggtggtaac tcccgtgagg
     7021 ccgttattcg tttcccgaaa gagaccggac agaagccggt ttatgtgtcg gtgacagatg
     7081 ttcttacccc ggcacaggta aaacagcgtc aggaggaaga aaagcgtcgc cagcaggcat
     7141 gggacgccgc tcatccggaa gaggggctga aaagagacta tgataaagcg aaagctgagc
     7201 tggatgccga agataaaaat attgcgacct taaacagccg cattgcatcg acagagaagg
     7261 cgctccccgg tgcaagggct gctgttcagg aagccgataa aaaggtaaaa gaggcagagg
     7321 caaataaaga tgattttgtg acttataacc cgcctcatga atatggctcc gggtggcagg
     7381 atcaggttcg ctatcttgat aaggatattc agaatcagaa tgagaaatta aaagcggctc
     7441 aggcatcttt aaacgcaatg aatgaatcct tatccagaga taaggctgca cttaccgggg
     7501 cgatggagag ccggaaacaa aaggagaaaa aagcgaagga tgcagaaaat aagttaaatg
     7561 aggaaaagaa caagcctcgc aaggggacta aagactacgg ccatgattat catccagtac
     7621 ctaaaacaga agacataaag ggacttggag agttaaagcg aggtgatcct aaaacaccta
     7681 aacaaggtgg tggtggtaag cgggcccgat ggtatggcga taaaaaacgt aaaatttatg
     7741 aatgggattc ccagcacggt gagcttgaag ggtatcgtgc cagtgatggc gaacacctag
     7801 gcgcatttga tcccaaaacg ggtaagcagg ttaaagggcc agatccaaaa cggaacatta
     7861 aaaaatatct ttgagaggta aatatggggc ttaaattacg tttgacatgg tttgataaga
     7921 aaactgaaga gtttaaaggg gaagagtatt ctaaagactt tggtgacgat ggttctgtca
     7981 ttgaaagctt gggaatgcct ttaaaggata atattaataa tggtgagttt gatttgaaga
     8041 aagaatgggt tcctttacta cagccatttt ttaaaaatca aataaaacct gatgtggata
     8101 attgttatat agcttttgat tatcgggacg gtaactggta acaaaaaatt atcaaggatg
     8161 atttgaaata ctgacttccg gtgttcaggg atgaatgccg gagtctttaa atacagggaa
     8221 agtatatgaa aaaggtaaag actatttttc tttttatatt gattgttgct ggctttcttc
     8281 tggccgcatg tcaggcaaat tatatccggg atgttcaggg cggaacggtg gcaccatcgt
     8341 cctcctctga actgacgggg atcgcggttc agtagaaaag atcaaaggat cttcttgaga
     8401 tccttttttt ctgcgcgtaa tcttttgccc tgtaaacgaa aaaaccacct ggggaggtgg
     8461 tttgatcgaa ggttaagtca gttggggaac tgcttaaccg tggtaactgg ctttcgcaga
     8521 gcacagcaac caaatctgtc cttccagtgt agccggactt tggcgcacac ttcaagagca
     8581 accgcgtgtt tagctaaaca aatcctctgc gaactcccag ttaccaatgg ctgctgccag
     8641 tggcgttttg cgtgctttcc cgggttggac tcaagtgaac agttaccgga taaggcgcag
     8701 cagtcgggct gaacggggag ttcttgctta cagcccagct tggagcgaac gacctacacc
     8761 gagccgagat accagtgtgt gagctatgag aaagcgccac acttcccgca agggagaacg
     8821 gcggacaggt atccggtaaa cggcagggtc ggaacaggag agcgcaagag ggagcgacct
     8881 accggaaacg gtggggatct ttaagtcctg tcgggtttcg cccgtactgt cagattcatg
     8941 gttgagcctc acggctccca cagatgcacc gaaaaagcgt ctgtttatgt gaactctggc
     9001 aggagggcgg agcctatgga aaaacgccac cggcgcggcc ctgccgtttt gcctcacatg
     9061 ttagtcccct gcttatccac ggaatctgtg ggtaactttg tatgtgtccg cagcgcccgc
     9121 cgcagtctca cgcccggagc gtagcgaccg agtgagcgag gaggcgcaat ttccccggca
     9181 cctgcaagac ggtcaccgga cgcgctgatg tgggatattg tcccctgatt aagtctttct
     9241 attccctgac gatcaattat caggataggt gaaccatgtc ggtttttcgc tgaacatttt
     9301 acgttaacct atctctgttg cttttttctc cttgcggaga aatgcaagtc atccccgctg
     9361 gggcgtggct gacttcaggg gaaaagacct tgcttacgct ggctggacgc caccgacgca
     9421 cggtcttttc ccctctttct ggctctgtcc ggctggacgc cggccacgcc agaaattcac
     9481 ccggcccccg tcaggctcac gcctgaccac ccacaaaaat ccacggtgct ggacgcacct
     9541 gacgaagcag caaagctgct cctgattttg cgggtggtca gccgctcgct atggcgaact
     9601 ctgacggggg ccggaacacc ggaaaagacc gcactggggt gccgcctttt cctctcacac
     9661 gctggggcgt gctcgttgtt cctccggggc tgcgcctttt ccgcatgggg cggaaagagg
     9721 ctggcctccg cggtgaaagc tgaacgcaat caccacggac gtgaccgccg ccgccatgcc
     9781 ttggggcatg tccgcggctt gtgcagcggg ggctgcgcat cacgccggta aacccgtcgt
     9841 ctgaaggaga gagattgcat gtttaatacg gattcgcttg cctggcagtg gcatatcccg
     9901 gagctggccc ggcagattta cccgtgggtc agctggctgt accgggagta ttacagtctg
     9961 gccggttttt ttctggttat cggcctgttt ttcggtctgg ctgaactctg ggccggccga
    10021 atgcgttttg tggggctgag ccataaggcg cggcgggagt
//
