LOCUS       NZ_CP040109             6042 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli O157:H7 strain MB9-1 plasmid pMB9_2, complete
            sequence.
ACCESSION   NZ_CP040109
VERSION     NZ_CP040109.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05360217
            Assembly: GCF_013343635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Bono,J.L.
  TITLE     Comparison of 54 complete closed STEC O157:H7 genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Bono,J.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2019) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 205.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 02:26:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,561
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 136
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 298
            CDSs (without protein)            :: 298
            Pseudo Genes (ambiguous residues) :: 0 of 298
            Pseudo Genes (frameshifted)       :: 112 of 298
            Pseudo Genes (incomplete)         :: 166 of 298
            Pseudo Genes (internal stop)      :: 73 of 298
            Pseudo Genes (multiple problems)  :: 46 of 298
            Pseudo Genes (short protein)      :: 4 of 298
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040109.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="MB9-1"
                     /serotype="O157:H7"
                     /isolation_source="cattle feces"
                     /db_xref="taxon:83334"
                     /plasmid="pMB9_2"
                     /geo_loc_name="USA:Louisiana"
                     /collection_date="2002"
                     /collected_by="USMARC"
     gene            complement(join(5870..6042,1..85))
                     /locus_tag="FDZ86_RS27895"
                     /old_locus_tag="FDZ86_28760"
     CDS             complement(join(5870..6042,1..85))
                     /locus_tag="FDZ86_RS27895"
                     /old_locus_tag="FDZ86_28760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001508827.1"
                     /translation="MPKKTSKPNDLSNTINNIKKEINSGFTELLNRVEALEASDAQHSM
                     AIRDLQIQARAARGDKRMDIARDFGLSEGRISQIVNAGRN"
                     /name="hypothetical protein"
     gene            630..1340
                     /locus_tag="FDZ86_RS27900"
                     /old_locus_tag="FDZ86_28765"
     CDS             630..1340
                     /locus_tag="FDZ86_RS27900"
                     /old_locus_tag="FDZ86_28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177388.1"
                     /translation="MLYWKRMPSLWIGIKISEFNDLDTAKAIAALKIYLTFCLFCKESD
                     SGQRTVELTFSNLCEIASLSRSLVNEGLKILYAKELIKNLSTTVRKKIYTIDVLEKHDD
                     GWCKLPFKGIVGEDGKISAFQSMHNRYPFELLALQTYMYLLYARDNRNEYTLARKKTIC
                     KKLKCKLPELNKAITYLIHIGLLDRIVKKSIENKPLEMFHDSCYFYFRAGSNSALTYKR
                     KSEVLNLSEEDLPF"
                     /name="hypothetical protein"
     gene            complement(1452..1967)
                     /locus_tag="FDZ86_RS27905"
                     /old_locus_tag="FDZ86_28770"
     CDS             complement(1452..1967)
                     /locus_tag="FDZ86_RS27905"
                     /old_locus_tag="FDZ86_28770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
                     /protein_id="WP_000675353.1"
                     /translation="MKFYTISSSYIKYLKDFDDKVPNSEDPTYNNPKAFIGIVLEIEGH
                     KYLAPLTSPKAWHANVKESSPAFFKLHENGVPDNQLGLINLKFMIPIIEAEVSLLDLDS
                     MPDTPYKRMLYKQLQFIRVNEDKISEKSKLLRNLALQGRMQGTCDFAVLEEKYQHFGKK
                     PEDMEIDD"
                     /name="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
     gene            complement(3251..3442)
                     /locus_tag="FDZ86_RS27910"
                     /old_locus_tag="FDZ86_28795"
     CDS             complement(3251..3442)
                     /locus_tag="FDZ86_RS27910"
                     /old_locus_tag="FDZ86_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165987.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGETL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3507..3830
                     /locus_tag="FDZ86_RS27915"
                     /old_locus_tag="FDZ86_28800"
     CDS             3507..3830
                     /locus_tag="FDZ86_RS27915"
                     /old_locus_tag="FDZ86_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001508824.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVVALMAIERELRSLRQVVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4039..>4470
                     /locus_tag="FDZ86_RS28710"
                     /pseudo=""
     CDS             4039..>4470
                     /locus_tag="FDZ86_RS28710"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4508..5026
                     /locus_tag="FDZ86_RS27925"
                     /old_locus_tag="FDZ86_28810"
     CDS             4508..5026
                     /locus_tag="FDZ86_RS27925"
                     /old_locus_tag="FDZ86_28810"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_043991090.1"
                     /translation="MSNLLRTGAEFEKKLKERAESTEKMLNDEFSRLGESVSEAVTSNE
                     TKIKGAIARFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVISFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            5033..5266
                     /locus_tag="FDZ86_RS27930"
                     /old_locus_tag="FDZ86_28815"
     CDS             5033..5266
                     /locus_tag="FDZ86_RS27930"
                     /old_locus_tag="FDZ86_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029401705.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5579..5860)
                     /locus_tag="FDZ86_RS27935"
                     /old_locus_tag="FDZ86_28820"
     CDS             complement(5579..5860)
                     /locus_tag="FDZ86_RS27935"
                     /old_locus_tag="FDZ86_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177387.1"
                     /translation="MKNEDDYKTGWTTQTTNPATGKKCSGGAARNLRIYQAGGANSVRV
                     KAAIEGVQSIQPIIDMQQSQIEQQQTQIAMLTQSLSQAINELTKSRNQ"
                     /name="hypothetical protein"
     Typing          3089..3170
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(2272..2789)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.792"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3821..5343
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.762"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 actcagtaaa acctgagtta atttctttct ttatgttgtt gatagtgttg cttaaatcgt
       61 ttggttttga tgttttttta ggcatggcat atttctctat actcggcggc taagtccgct
      121 aaagattttt agttttgtta aagttgtaag gtagggatta ttgctccttt tggcaaggtg
      181 aatttaatga gataaagaaa tttagtgctt tatgcagggt ggcttgcaag gcacaacgct
      241 ttgtttgttc tgatctaaag tcctaatgtg aaaacattta acgtaaccag aaaagcatca
      301 gcactgcatt tacggttcac catgaaccat aatatgattt ctaagaatac caaaagcaag
      361 ataaaatatc ttatataaca tatagatatc tttcttttgc cttatcttat ctttctagat
      421 agatttttgt gaaggcgtta caacgccttc acagcctgag ataatcaaaa aaacaaagct
      481 atcaggaggg ataaaaaatg tttgagaaag gtgtgttttt ggctgaaagg cttgcgaagg
      541 tcgcagtggt gctaactaat ctggtgctat ctatgtctgt attggggttg gtctatttca
      601 tttttcaaat tctgggaatg aggtgattta tgctgtactg gaaaagaatg ccttcactat
      661 ggataggtat caaaatatct gaattcaacg atcttgatac agcaaaagcc atcgccgcgt
      721 taaagattta tttaacattt tgccttttct gcaaagagtc agattcagga cagagaactg
      781 ttgagctaac ttttagtaac ctatgtgaga tagcatcatt aagccgatcg ttagttaacg
      841 aagggcttaa aattctttat gcaaaagagt tgataaaaaa tttatcaacg acagtacgta
      901 aaaaaattta taccatagat gtattagaga agcatgatga tggttggtgt aagcttccct
      961 ttaaaggaat tgtaggtgaa gatggaaaaa taagtgcatt tcaatccatg cacaacagat
     1021 atccatttga gttgctagct ttacaaacct acatgtacct tctttatgct cgagataata
     1081 gaaatgagta tactcttgcc aggaaaaaaa caatatgtaa aaaactgaaa tgcaaactac
     1141 ctgaactaaa taaagctatt acctatctta ttcatatcgg tttacttgat aggattgtta
     1201 aaaaatcaat agaaaacaag ccgttggaaa tgtttcatga ttcatgttac ttctacttta
     1261 gagcgggaag taacagcgct ctcacttaca aaaggaaatc agaggtgctt aatctttcag
     1321 aggaggactt gcctttttga ttgcttctcg aaatcaaaat tagaatatct cattcttata
     1381 ttggatgaga gcaaaaaaaa taggtccaaa accgcttgat tttggacctt caattccata
     1441 aaaaagaatg gttaatcgtc tatttccatg tcttcaggtt ttttaccgaa atgttgatat
     1501 ttttcttcta acacggcaaa gtcgcatgtt ccttgcattc tgccttgcaa ggcaaggttt
     1561 cttaatagtt ttgatttttc agatatttta tcttcattta cacgaataaa ttgtaactgt
     1621 ttatatagca ttcttttgta aggcgtatca ggcatgctat ccagatcaag taaagacact
     1681 tcagcttcga ttattggaat cataaatttc agattaatca atccaagctg attatcaggg
     1741 acaccgtttt catgaagttt gaagaaggcg ggtgatgact cttttacatt agcatgccat
     1801 gcttttggcg atgttaaagg tgctaaatat ttatgtcctt caatctctag tactatgcca
     1861 atgaaagcct taggattgtt gtatgtagga tcttcgctat tgggaacttt gtcatcaaag
     1921 tccttcaggt attttatgta actgcttgat attgtgtaaa atttcatttt ttctcgtaaa
     1981 aaaggcgact gtgtatagtc gcctttttta ctttattact ttaaggtagt aaatgaccta
     2041 aatttctagc tgcacttaaa ggtagcaaat cacctgaatt tctagctgca cttaaaggta
     2101 gcaaatcacc taaatttcta gctgcactta aaggtagcaa atcacctaaa tttctagctg
     2161 cacttaaagg tagcaaatca ccttgaacta agtatacatg atcttcgctt gggcttacaa
     2221 ctaggtgctt agcatgggat gttcagtcaa atgacggcac taatcaaaag gatcttcttg
     2281 agatcatctt tttctgcgcg taatctcttg gtctgaaaac ggaaaaacca cccggcaagg
     2341 tggttttttc gaaggttcag taaaccggca agtttctgga ccgtggtaac tggctttcgc
     2401 agagcacagc aaccaaatct gtccttccag tgtagccgta ctgaggcgca cacttcaaga
     2461 gcaacctcgt gtttagctaa caaatcatct gcgaaccaag ttaccaatgg ctgctgccag
     2521 tggcgctttg tcgtgtcttt ccggtttgga ctcaagtgga tagttaccgg ataaggcgca
     2581 gcagtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc
     2641 ggaaccgagt gtcaggcgtg gaatgagata aacgcggcca taacagcgga gtgacaccgg
     2701 taaaccgaat ggcaggaaca ggagagcgca cgagggagcc accaggggga aacgcctggt
     2761 atctttaagt cctgtcgggt ttcgccacct ctgacttgag cgtcggtttt tgtgatgctc
     2821 gtcagggggg cggagcctat ggaaaaacgc cagcggcgtg gccttttccg ggttcttgtt
     2881 ttttgctcac atgtttcttt tggtgttatc ccctgattct gtggataacc gcattaccgt
     2941 ttttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgtagcg agtcagtgag
     3001 cgaggaagcg gaagagcgtc tgatgctgca ttttctcctt acgcatctgt gcggcatttc
     3061 acacccggca tggcgcactt ttcatacaat ctgcactgat gccgcatggt taagccagta
     3121 tacactccgc tatcgctacg tgactggttc agggcttcgc cccgaaaccc cctgacgcgc
     3181 cctgacgggc ttgtctgctc ccggcatccg ctcacagaca agctgtgacc gtctccggga
     3241 gctgcttgtg tcagagggtt tcaccgtcat ccccgaagcg tgcgaggcag ctgcggtaaa
     3301 gctcatcggc gtggtcgtga agagattcac agatgtctgc ctgttcatcc gcgtccagtt
     3361 cgttgagttt ctccagcagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
     3421 cggttttttc ctgtttagtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
     3481 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
     3541 cccggttact ggaacgctgt gagggtaaac aactggcggt atggatgcgg cgggtctgcc
     3601 tgggtgagcc ggttgcccgt tccgggaagc tgccgacact ggcaccgccg ttactgcgtc
     3661 agctggctgc catcgggaat aaccttaatc agacggcgag gaaggtgaac agcgggcagt
     3721 ggtcttccgg cgaccgggtt caggtggtgg tcgcactgat ggccatcgag agagagttgc
     3781 gcagcttacg acaggtcgtc agggaacagg gagcgcggga tgatagttaa gtttcatgcc
     3841 cggggaaaag gtggtggcag tggtccggtt gattaccttc tggggaggga gcgtaaccgg
     3901 gaaggtgcaa cggtacttca gggtaacccg gaagaagtcc gggagcttat tgatgccacg
     3961 ccatttgcga agaaatacac gtccggtgtt ctgtcgttcg cagagaagga actgccgccg
     4021 ggaggacgtg aaaaagtgat ggagagcttt gagcgcgtac tgatgcccgg tctcgaaaaa
     4081 aatcagtaca gcattctgtg ggtggagcac caggacaagg ggcgtcttga gctgaatttt
     4141 gtcattccga acatggagct acagagcgga aaacgcctcc agccgtacta cgaccgggca
     4201 gacaggccga gaattgatgc ctggcagacg ctggtgaatc accattacgg gctgcatgac
     4261 cccaacgccc cggagaaccg caggacgctg acactccctg ataacctgcc ggaaacgaaa
     4321 caggcgcttg ctgagagcgt tacacgaggt ctggaggtgc tttaccatta cggagagata
     4381 aaaggccgtc aggatgtgat tcaggcgctc acagaggcgg ggcttgacgt ggtcagggtg
     4441 acgcgaagca gtatcagcat tgcagacccg aacggcggga ggaatatcag actgaaagga
     4501 gcgttttatg agcaatcttt tacggacggg cgcggagttc gagaaaaagc tgaaagagag
     4561 agccgaatct acagagaaaa tgctgaacga cgaattcagc aggctcggcg aatctgtaag
     4621 cgaggctgtg acctcaaacg agacgaaaat caaaggcgct atagcccggt ttacggcctc
     4681 gacggaggaa tcgctggaaa aacaccggga aggggtgaaa gaggcgatga tgcagcacag
     4741 gagggacgtg ttaaagctgg cagggaatac gggcatgatg ttactgggga tagtctttct
     4801 cctgtttacc gcgagtggcg ggacgctctg gtatcttggc gggaggatac aggcgaacct
     4861 ggaagatatc aggaagcagg aagagacatt acagaaactg aacgcgaaga catggggcgt
     4921 ggagtttgtg caggacggga acaggaaatt tctggtcctt ccttacggga agtcagcaga
     4981 ggtgatttcc tttcagggga aggaatgggt acatctgaag gagtgataca gagtgacaga
     5041 gctggaaaca catttgctga acgccttaga gcagctgcaa caggactata tgcagcggct
     5101 gagcgaatgg gagagcgcct tcgtggaatt gcagaagatg ttttcgctta cgcaacggga
     5161 caacgcgatg ctgaacgagc gggtcatgca gttgagtcag caggtgcagc acttgagcga
     5221 gcggacagaa cgcttgagcc agttatacag cgagaactgg agataagaga ggaacggttg
     5281 atacatgagc gcgaacatgc gttatccctg gaacgggaac gcacgctgga tggtccttcg
     5341 ttgtcgctgt gatggcgaaa ctataaaaaa tggcagattc agtggatttt gacgggctaa
     5401 tgtagtctgc accatctggt tgcatagata ttcatacggt taaaatttat caggcgcgat
     5461 cgcggtagct tttcgggtga tttgttgttg gttttggctg actgccgccc cgttcgcggc
     5521 gaagctgtcc ggggcggtgc gggcaacgga tgttatggta aataacatcc tttatagcct
     5581 attgattcct gctttttgtt agctcattga ttgcctgaga caaagattga gtcagcattg
     5641 ctatttgagt ttgttgttgc tcgatttgtg actgttgcat atcgatgatt ggctgtattg
     5701 actggacacc ctcgattgca gcttttaccc gcacggagtt tgctccaccg gcttgatata
     5761 tccttaagtt tctggctgcg cctccagaac attttttccc tgttgccggg tttgttgtct
     5821 gagtcgtcca tcctgttttg tagtcgtctt catttttcat aagtctgtct tagttacgac
     5881 ccgcattaac gatctggcta attcgtccct cgctgaggcc aaagtctctt gctatatcca
     5941 tgcgcttgtc tccgcgagcg gctctagcct gaatttgtaa atcacgtatc gccatgctgt
     6001 gttgtgcgtc actagcctct aaggcttcta cgcgattaag ta
//
