LOCUS       NZ_CP040088            11194 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter baumannii strain VB35575 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP040088
VERSION     NZ_CP040088.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11571817
            Assembly: GCF_005280715.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Veeraraghavan,B., Vijayakumar,S., Vasudevan,K., Narasimman,V. and
            Venkatesan,M.
  TITLE     Carbapenem resistant Acinetobacter baumannii_VB35575_hybrid assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11194)
  AUTHORS   Veeraraghavan,B., Vijayakumar,S., Vasudevan,K., Narasimman,V. and
            Venkatesan,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2019) Clinical Microbiology, Christian Medical
            College, Ida Scudder road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.8; Unicycler hybrid v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 31.0x
            Sequencing Technology :: IonTorrent; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 01:27:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,893
            CDSs (total)                      :: 3,812
            Genes (coding)                    :: 3,658
            CDSs (with protein)               :: 3,658
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 92 of 154
            Pseudo Genes (incomplete)         :: 56 of 154
            Pseudo Genes (internal stop)      :: 18 of 154
            Pseudo Genes (multiple problems)  :: 11 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040088.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11194
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="VB35575"
                     /isolation_source="blood"
                     /host="Homosapiens"
                     /db_xref="taxon:470"
                     /plasmid="unnamed1"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2018"
                     /collected_by="Christian Medical College"
     gene            1..936
                     /gene="repM"
                     /locus_tag="FDN01_RS19530"
                     /old_locus_tag="FDN01_19530"
     CDS             1..936
                     /gene="repM"
                     /locus_tag="FDN01_RS19530"
                     /old_locus_tag="FDN01_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001292329.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLREASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YESQSGIIFLRFTQDIVPLITRLEENFTKYELQQVSRLSSSYAIRLYELLIQWRSAGKT
                     PLFDLSIFRQQLGVKPHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFTFKQKKMSNLPIKNKRDPDTIDIFSKMTDAQRHLFSHKLSELPEMGKYSHGTESYPQ
                     FAVRIAEMLQNPEKFKELYPYLQKVGFKAA"
                     /name="repM"
     gene            1014..1532
                     /locus_tag="FDN01_RS19535"
                     /old_locus_tag="FDN01_19535"
     CDS             1014..1532
                     /locus_tag="FDN01_RS19535"
                     /old_locus_tag="FDN01_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000839337.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPVQKQSVKETVKVDSPTSAEVLLLRQQIDLLKNQLDDAKNRESFYQNQIETMQRLL
                     EAPKTNTTQSDDPGSTQAESTVEEKEDVIAMPPKESEVIENKRIPVPEHIEPEPEKRGF
                     WSRFFKPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            1742..2041
                     /locus_tag="FDN01_RS19540"
                     /old_locus_tag="FDN01_19540"
     CDS             1742..2041
                     /locus_tag="FDN01_RS19540"
                     /old_locus_tag="FDN01_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            2309..2596
                     /locus_tag="FDN01_RS19545"
                     /old_locus_tag="FDN01_19545"
     CDS             2309..2596
                     /locus_tag="FDN01_RS19545"
                     /old_locus_tag="FDN01_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438827.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            2583..2897
                     /locus_tag="FDN01_RS19550"
                     /old_locus_tag="FDN01_19550"
     CDS             2583..2897
                     /locus_tag="FDN01_RS19550"
                     /old_locus_tag="FDN01_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            3025..5436
                     /gene="znuD2"
                     /locus_tag="FDN01_RS19555"
                     /old_locus_tag="FDN01_19555"
     CDS             3025..5436
                     /gene="znuD2"
                     /locus_tag="FDN01_RS19555"
                     /old_locus_tag="FDN01_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            5741..6203
                     /locus_tag="FDN01_RS19560"
                     /old_locus_tag="FDN01_19560"
                     /pseudo=""
     CDS             5741..6203
                     /locus_tag="FDN01_RS19560"
                     /old_locus_tag="FDN01_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /name="DIP1984 family protein"
     gene            complement(6529..6738)
                     /locus_tag="FDN01_RS19565"
                     /old_locus_tag="FDN01_19565"
     CDS             complement(6529..6738)
                     /locus_tag="FDN01_RS19565"
                     /old_locus_tag="FDN01_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069474.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCEYFS
                     HCKRPSYYIDIFDNAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(6731..7033)
                     /locus_tag="FDN01_RS19570"
                     /old_locus_tag="FDN01_19570"
     CDS             complement(6731..7033)
                     /locus_tag="FDN01_RS19570"
                     /old_locus_tag="FDN01_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001129974.1"
                     /translation="MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7026..7382)
                     /locus_tag="FDN01_RS19575"
                     /old_locus_tag="FDN01_19575"
     CDS             complement(7026..7382)
                     /locus_tag="FDN01_RS19575"
                     /old_locus_tag="FDN01_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269904.1"
                     /translation="MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSNKKRFYTEMLAIADEQ
                     YALHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7948..8247)
                     /locus_tag="FDN01_RS19580"
                     /old_locus_tag="FDN01_19580"
     CDS             complement(7948..8247)
                     /locus_tag="FDN01_RS19580"
                     /old_locus_tag="FDN01_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            complement(8364..9125)
                     /locus_tag="FDN01_RS19585"
                     /old_locus_tag="FDN01_19585"
     CDS             complement(8364..9125)
                     /locus_tag="FDN01_RS19585"
                     /old_locus_tag="FDN01_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425105.1"
                     /translation="MEMEKAKKRGRPAQLLQIAELHAFVEFLEQQEQLSDLQSQVLKAL
                     NSVDCNFEGLTQTDQVLVKEALKPYREHLKLKLLFEELNNLPLKTEYEQKFLDLYELFQ
                     KNALDQMELNILKTLATRYLNFKAQKLEYSDLELYLSQLQKKDAGKKRKAENQRKFELG
                     GAVLVAFKKLNIDISNDTPQQITNRIVNTTKFHNEVRKSLIFKDVKTYENEYFKANKLF
                     IQVLEGLHTWQKGGELLSVIEIKKALEKGEE"
                     /name="hypothetical protein"
     gene            9430..10599
                     /locus_tag="FDN01_RS19590"
                     /old_locus_tag="FDN01_19590"
     CDS             9430..10599
                     /locus_tag="FDN01_RS19590"
                     /old_locus_tag="FDN01_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002134206.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIDFWQAADTYERANAKAYMEYEIALPNEFTSEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHEDYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENNPFSSLEVMYYLNYLIKNATNKDKLKNDLNEIIKNQGPILKQEAWSIYEMNEKLEDS
                     RRNSTPCFEFEVHSLPEYVLPIQNTPPKRTQSEPVQPKADDYDYGGPGF"
                     /name="MobA/MobL family protein"
     Typing          2..936
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9509..10596
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagtgaat taatcgtaaa ggataatgct ctaattcaag caagttatac cttagataca
       61 gttgaacaaa gactgatcct attagccatt gctgaagctc gagaaactgg acatgggata
      121 actgaaaata gtcttttaga agtacatgca agtagttata taaatacttt taatgtcgag
      181 aaacataccg cttataccgt actcagagag gcatctaaaa gcttatttga tcgctatgtc
      241 acataccatg acattaatcc taagacaggt aaggatcgta gctttcattg tcgttgggtc
      301 gacaaaattg ggtatgaatc tcaatcagga attattttcc tacgattcac ccaagatatc
      361 gttccactca taactcgact tgaagaaaat ttcactaaat atgaattgca gcaggtttct
      421 aggttaagta gctcatatgc tattcggcta tacgagctat taattcaatg gagatctgcc
      481 ggaaaaacgc cactttttga tctatctatc tttagacaac aacttggtgt caaacctcat
      541 caatacaaaa caatgagtaa ctttaaaaca tatgttttag attttgctct taagcaggta
      601 aatgagttaa ccgatataac agctaaatat gagcaacata aaaaagggcg ttctatttca
      661 ggtttttcat tcactttcaa acagaaaaaa atgagtaatc tgccaataaa aaataagcgt
      721 gacccagaca ctatagatat tttctcaaaa atgacagatg ctcaacgcca tctgttttcc
      781 cacaaactgt cagaacttcc tgaaatggga aagtattctc atggtacaga aagctatccg
      841 caattcgctg tacgcattgc agaaatgctt caaaacccag aaaagtttaa agaactctat
      901 ccttatctcc aaaaagtagg cttcaaagct gcatagaaga tagacttgct gatttgtcta
      961 ttagacagtc taatagacac taatttttta caagattagg cagagattta cacatgaaaa
     1021 gccttacagt tcttgagctt tctaagcttt acaatattaa tcgacaaacc atttacaaca
     1081 atataaagaa aggtatttta agtaaaaatt ctgacaataa gattgatctt gcagaagcga
     1141 tccgggtatt tggtgagcca gtacaaaaac agagtgtaaa agaaactgta aaagtagaca
     1201 gtccaacttc ggcagaagtt ttactcctta gacagcagat agacctacta aaaaatcagc
     1261 tagatgatgc aaaaaataga gaaagcttct atcaaaatca aattgaaact atgcaacgcc
     1321 tattggaagc tccaaaaacc aatacaacac aatctgatga tcctggatca acacaggcag
     1381 aatccacagt agaagaaaaa gaggacgtca tagctatgcc tcctaaggag tcagaggtta
     1441 tcgagaataa gcgtattcca gtccctgaac atattgagcc agaaccagaa aaaaggggct
     1501 tctggagccg ttttttcaag ccctatggat gataggacta tttaatcaga ctaatcaaaa
     1561 ccgtggaaca atgatcaata attgatcgtg aaacataaaa aatcactaaa acagcctgag
     1621 cgtgactgct taggctgttt ttacatccat aagacattgt tttaaaatgc tttttacaaa
     1681 gaatttcgta taaggtgtat tatgttaatt ttagggaaac ttaattctat aggtgatacc
     1741 aatggacaaa aagatagaag agaaaactaa agatattaat gaaaaaattc aaaaattgga
     1801 tttagctatt caagaactaa tagaaactac agacaatttt tttattaatg ataagtacta
     1861 tggaattgaa tgtgctttta ttttagacca tgcactaaat attaaaatgc actttttcaa
     1921 caagtatcca gcatttgcac cacctcactt acgaggagta gaaaattatg atcgaattat
     1981 acatattaga gaaaataatc ctgaagataa gctagaacaa atttctcctc ttaaagacta
     2041 aattttctaa tcttgtactt agagtcccct actatttaac ataatggata ttatacgaag
     2101 tacacttgta tattaattta aatatcaact acttaataaa aatataaaaa tccaaaaatg
     2161 cccaaaaagc cgactttgaa acaagtcggc ttttttatga tcgattttga tagtttttgc
     2221 caataaatct gactaaaaat tacgcttctc aacttgagtg atacaatttc gtgatacatt
     2281 attgtatata caaatttgaa tattttttat ggaacagtat tttgaatggg atgaggctaa
     2341 gaatcgaaag aatcagaaaa agcatgatat ctctttcgaa actgcaagcc ttgtttttga
     2401 agatccatta cggatctcaa tccaagacag atatactaat ggtgaagaac gttggcaaac
     2461 cattggacga gttaaaggcg tactaatgtt attggtggct cacaccattt ttgatgaaga
     2521 tgactgtgaa atcatccgaa tcattagtgc aaggcaagtc actaaagcgg agcgaaacaa
     2581 atatgagcat ggttagatac tcacacaaag agctgaatga gaaatttggt gagaagcaag
     2641 atgctgaaat tcaacgcttg cttgctaaag gcacggttcc tgatgatcag ctagatctat
     2701 cagatattcc tgaaattaca gactggagca atgctgtacg ccaaaatcaa ttttaccgcc
     2761 cagtaaagca acaaacttca attcggttag atgctgatgt acttgcttgg ttcaaagcac
     2821 aaggcaaagg ttatcaaaca cgtatgaatg aaattttacg agatgctatg ctaaaagaat
     2881 taaaaaatca tcaataagaa tgggagctta ggctcccatt tttgacttca tttttagggg
     2941 ggattaccat tcaaaaattt aaatagtaga ttttataaat ttttaagtgt tatattataa
     3001 tataacatta aatttggttg ttttatgcta aataaaagta aactattttt agctttaatt
     3061 actttagggg cctcgaaaat tttattggca gctgaagggc ctgttactac cttaaataca
     3121 atagtattaa ctgcacaatc tgatgagtta gggtctgagc tgttaggcaa aagtctaaat
     3181 gttagtaatc agtttattga tactagtaaa cttaaacagc gttcgacaac tctaggggat
     3241 gccttgggta cggaattagg aattcattct aatcaatatg gaggtggagc atctactcct
     3301 attattcgtg ggcaggaagg aaaacgtatt aaagttttac agaataatgc tgacgtgttg
     3361 gatatgtcta atatgtctcc agatcacgct gtcacagttg aaccaagttt agctaagtct
     3421 attgaaatta tacgtggtgc aagtaccctt ttgtatagtt caaatagtgc ggcaggggta
     3481 gtcaatgtca ttgactataa aattccaacc caaatgcctc aagacggatt agagggtaat
     3541 actacattac gttttaatac tggcagtaat gagaagttga ccacagcagg tgtaacagtt
     3601 ggcttaagcc cacatgtagc tttacgggca gaagggttat atcgtaatgc tggtaactat
     3661 aaaactcctc attaccagtc tagctcttat aattctttag aagatcttga aaaccaaaac
     3721 agtatttata aaaatttaaa atatttacca gaaagctggg cagagtcgag agtaggtacg
     3781 cttggattat cctggataga cgataatact tatttaggag tttcttatac acatcgtcat
     3841 gatgaatatg gtttaccagc ccatagccat ttatatgaag gttgtggtgc ctctgcaatc
     3901 ggtatcgatt cacgaattag tggattaaaa aattatcttt tatattatcc tcaattgatg
     3961 gatgagcaag atattaatta cataaatcca cgtcctgatt gtcatcagca taatcatatt
     4021 catgaaacta atttctctca taatgctcct tatattgatt taaatactcg acgttatgat
     4081 gtgcgtggag aattcactca accatttacc ggtattgaca aaattcgtac ctcattgagt
     4141 tatatagatt attttcacaa tgaactcgag ggagataaga ttactaactt ttttaaaaat
     4201 acaggaaaag taggccgcat agagttatca catcagccat taggggaatt aaccggtatc
     4261 ttggggctac aatatttgga acaggataat agtgctttat caccagtcca ttctcaagaa
     4321 ggccatacca cctatctaga taatcagcag ctattaaatc gaaatgtcac taaaaacttt
     4381 agtgtatttg gcctagaaaa atataactgg aacgacttta ctttcgaact aggtgctcgt
     4441 atagaaaagc agaaagttag tatggattat gatatagaaa aaattaaaga ttcaatgaaa
     4501 ccttggccta ataaatataa cagtccttat gtagaaaaaa ataataaaat cagggctcaa
     4561 aatttaaaat ctattttaga agctgttcaa cctaacaaag aaacagcttt ttcttatgca
     4621 ggtacagtcc actggagatt tgctccaaat tatatcttat cattaacagg tacacatcaa
     4681 gaaagattac ctaatgcaca agaaatgtat actcatggta tgcatctcgc tactaattct
     4741 tttgaaatag gtaaccgctt tctaagaaaa gagaaatcca ataatttaga aatcagtctg
     4801 gcctataagg atgatttact tgattatcaa atcagtactt actattatga ttttgataac
     4861 tatatatatt tacagacact caatgaagtt ttaggaacta caaaagttag agatcagcat
     4921 acactacgta ttaatcatta tagccagtct gctgctaatt tttatggatt agagggcaac
     4981 atagggtatc aatttaattc tgtttatcac ggctctcttt ttggagatta tgtgaaaggc
     5041 cgtttaacaa atttacctga tgctgtaatt gcatacgata tctggaatag agaaccgact
     5101 ttagctcccc aaaaggatcg ttacactcct agattacctc ctgctcgttt gggtacacga
     5161 ctaaaagctg attttgatga atcgcttaaa ggtgaaatag aatattatcg agtctttaaa
     5221 caagataata tttctaaatt tgagcaggtt acatcaggtt ataatatgct taatatgact
     5281 ttagcatata aaaataagct atctcacaca gaatatgatt tattctttaa agcgaataat
     5341 ttacttgatc agaaagtcta tgcccatgaa acatttttgc catatattcc ccaaattgga
     5401 agaaatttta gtttaggctt aaatcttaat ttctagataa gatactgtaa tcttctaaga
     5461 aaatttctta gaagattttt cttataaaat gattagatta ataattaagg taagcgtccg
     5521 ctgaatccca tacctatttt ttaattcgat ttaggtttcc agcagtgtgg cagtaactct
     5581 tcaatctggg tcactttatg tgtcggcagc cttttcagca ctgaatagcc accgattttg
     5641 tagacacctt ttagctagat aaaaagcaat ttttttgatc tttaaagaat gaggatataa
     5701 gctgtttaaa acttaattct aattggttaa taggtgaaaa atgaaattag cagaagctct
     5761 cttacttcga agtgatcaac aaaaaaaaaa ttatttcact aaaacaacgt atcaatgcaa
     5821 atgtattggt acaagatggt gatcaaccat ctgaagatcc aaatgaacta ctaaaacaag
     5881 tattttcttt aattcaagaa tttcaaaaat taagttatgc aattcatgaa actaatgctt
     5941 taacaaaatt aaatgatggc cgttctctac ttgctttatt gactttgcgt gatgaatttg
     6001 tagaacagca caaaacccta actgcagcaa taagtaatac atctcgtgaa tctgatcgtt
     6061 atagtacgcg ggaaataaag tggcataaag ttattccagt ttcatcatta caaaaacaag
     6121 ctgatgatat tagtcttaaa ttacgtgatt taaacgtgtt aattcagtcg actaattgga
     6181 aaattgattt gattgaagcc taatagtcct ttagatataa tcaagttgaa catactgggc
     6241 gagtacaaga tcccttactt ccacaacata ggattgtaaa taaagtgtag tggcttgcaa
     6301 agcaagtgga taagcttaaa attttaagta caaatcaatc catcacttgg cataactcaa
     6361 atctttgtgc aaacaaagtt acacgttact tataactacc atgtactgac agtatgttca
     6421 cctcaattac taaaaataaa gaaatgagca agcatcagct caactacatt tctattttta
     6481 attcatttcg gcatttaaca taaaatctct tatgcgaatc tagaaggatt aagctatttc
     6541 gtaatatttt atccaataag cattgtcaaa aatatcaatg taatagcttg gcctcttaca
     6601 atgtgaaaaa tattcacagc ataaatctct aataatttca atttcttcta ggctatcggc
     6661 agcattgact aaaaatataa acttgtctaa gttaacaagt aatttagtac tatttttcaa
     6721 aggtttatta ttagacattg aaccctatgt gttttcctgt tggaagctct acatcaatac
     6781 gaagttttcc acccattgct tcaacatact ttttcatcgt tgaaatttta agatcatgac
     6841 cacgattttc gattgctgat aatgacggct gtttaattcc aagggttgca gctagttctt
     6901 tctgggaaat ttctaactct tctcggataa ggtgaagctg actttctaaa agtaattcat
     6961 cagccatttt ttgaatacga gcttgactct ctggagagcg actagccaat aattcttgta
     7021 agctcttagc cattagattg atctcctaaa gttgagagat gaagtgcgta ttgctcatct
     7081 gcaattgcta gcatctctgt ataaaaacgc tttttattac tcttatcacc aatacacagc
     7141 acaatcgctt gtcttagggg atcgaatgca aaaaatgccc ttaacggttt accacgatgc
     7201 tgaacacgta gttccttcat attggtaaat ttagaatcgt agacggtatc tactaaagga
     7261 cgacctaaac taggcccttg ctgttgtaga acaaccaatg cagctaagac tttctcttgt
     7321 gttgcttcat cttgctgttc aagccattca ttaaacagat ccgttgtaat gactgtccac
     7381 atattacaat cacaatatag attacagtct atatattaaa tctgattgaa tgactttgca
     7441 aatggcaata aaaaatcccg acgtcatgtc gggatttttc ggtatggagc gcataggtag
     7501 ggctgactac ctgatggtca tggtgcctga ctgattatta ttgttgtttt aagtcattga
     7561 acatcctgtc catatagatc gatcataaga gcttcatcgg gggatagata cagacaattt
     7621 cgtgaaacgc tgtaagccaa aacgtacaaa aaataatcaa aatttatccc agtggttatc
     7681 cacagaaata gtggattctt ttgggtaaaa aagcttataa actctatgga attgctattt
     7741 ttgattattt tttaaacaat tttattggca agaagtatca aaattgagca taaaaaaacc
     7801 gacttgtttc taagtcggtt gaacgaggtc gttccagagg gtatgactca atgtaagcta
     7861 ctgatcttaa agtaatctta aagatccatt tcgcataata tccattatgt taaatagtag
     7921 gggactctaa gtacaagatt agaaaattta gtctttaaga ggagaaattt gttctagctt
     7981 atcttcagga ttattttctc taatatgtat aattcgatca taattttcta ctcctcgtaa
     8041 gtgaggtggt gcaaatgctg gatacttgtt gaaaaagtgc attttaatat ttagtgcatg
     8101 gtctaaaata aaagcacatt caattccata gtacttatca ttaataaaaa aattgtctgt
     8161 agtttctatt agttcttgaa tagctaaatc caatttttga attttttcat taatatcttt
     8221 agttttctct tctatctttt tgtccattgg tatcacctat agaattaagt ttccctaaaa
     8281 ttaacataat acaccttatg cgaaattcca atgtaagccc aaaatagaaa tgtccaactt
     8341 ctctaaaatc cctgctaaat aaactactct tcgccttttt ctaaggcctt tttaatttca
     8401 attactgaca ataattctcc gcccttttgc caggtatgta atccttctaa cacctgaata
     8461 aaaagtttat ttgctttaaa atattcattt tcatatgtct taacatcttt aaaaattaaa
     8521 gatttacgta cttcattatg aaactttgtc gtattcacaa tccgattggt aatctgttga
     8581 ggagtatcat tgctaatatc aatattgagt ttcttaaatg cgacaagaac agcaccacct
     8641 aattcaaatt tacgttgatt ttcagctttg cgttttttcc cagcatcctt cttctgtagc
     8701 tgactcaaat acaattctaa atcactgtat tcaagctttt gcgctttaaa attcagatac
     8761 cgtgtcgcca aggtttttaa aatatttagt tccatctgat ctagtgcatt cttttgaaaa
     8821 agttcataca gatctaaaaa cttctgttca tactcggttt taagaggcag attattcaac
     8881 tcttcaaaca gcaatttcaa cttcagatgt tcacgataag gttttaaagc ctctttgacc
     8941 agaacctgat ctgtctgagt tagcccctca aaattacaat caacactatt taaagccttt
     9001 aacacttgag attgaagatc agaaagctgt tcttgctgtt ctaaaaactc tacaaatgca
     9061 tgtaactcag caatctgtaa caactgagca ggtcgaccac gcttttttgc tttttccatt
     9121 tccatcatct gccgccaaat tttcagaact gcaacctctt taaatatggt tttaccatat
     9181 ttaaagaggg cagtctgaaa ataattattt aatataaaaa taaaaattct tttcaagaaa
     9241 ttttattttt atattaaata attcgcactt gacagtttct cctcatcgga gaaactagta
     9301 cgcaatgggg cgcttccccc ttgaccccct tcaaccggat ttttcagcca tacaggcatt
     9361 tcaaatccgg gcggttttca ttccgtttca ctgcataaaa accgaaccca caacccagca
     9421 gaggtgaaca tggccagcta ccacttcagc gtcaaaagca aaaataaggg ctatgcgacc
     9481 ggccattatc tctacatttc ccggctgatg cagtatgaag gcatccgcaa aaccagcaat
     9541 gagaccatag aacatattga acctggtcga tgtatgcctg cctttgtaaa agatcccatt
     9601 gatttttggc aggcagcaga tacgtatgaa cgagccaatg ccaaggccta catggagtat
     9661 gaaattgccc taccgaatga gttcacgtca gaacagcgta aaacgctcat agagacgttt
     9721 ttagacaaac atatagttcc acagcaatat ccgcactcat acgccataca caacgtaaaa
     9781 tcacgtatca gtggcgaaga tcagcctcat tgtcatctga tgttctcgct aaaagcggat
     9841 gacggcattg aacgcagcgc agaacagtac ttcaaacgct ataaccctca agatccagcc
     9901 aaaggcggtg ccaaaaaaat ccaacttcaa gatggccatg aagactattc cacttttttg
     9961 atctatatcc gaaaacaatg ggaaaatcat ttgaatgatg cccttgcaca gcactgcccg
    10021 accgtcacct acacactgga tggccaagac atcaccatca aaaatcaggt ttcagcagac
    10081 agctatgaaa aatataacga gatccacagc acgctatacc ttccagagcc aaaactaggc
    10141 gttggacaac aaaatgcgac agcggaatat ctcgcccaaa tccaaaaaat cagagaacac
    10201 aaccagcaag aacgagaact cgaagaacgc caaaagcaat tagatcaaca acatgaatat
    10261 ttttatagtg ctgaaaacaa cccattttca tcattggaag tcatgtatta cctgaattac
    10321 ttaatcaaga atgcaacaaa taaggacaaa ctaaaaaatg atttgaatga aattatcaaa
    10381 aaccaaggcc ctatacttaa acaagaagct tggtccatct acgaaatgaa tgaaaaacta
    10441 gaagactcaa gacgtaacag taccccatgt tttgagttcg aggtgcattc acttcctgaa
    10501 tacgtattac cgatacaaaa tacgcctccc aaacgcactc aatcagaacc agtacaacct
    10561 aaagctgatg attatgatta cggtggccct ggtttttaag ctcagttggt tttatccaaa
    10621 gtttacagag cgagtgtcta cgagcgaaca gacacaatta aaatttttgc catgcttttt
    10681 ccctgattac aaactcccct ttgctgctga gactgagata cagagcatcc cggagggtgc
    10741 gaacagattt aatttgcatc taggtcgagc gaagcgagta taaaaaactt atgagcgaag
    10801 cgaattccga gttgcttttg cttttttgaa gttatcacga gattaaccca aaaattgccc
    10861 cttcgaactg agcgtaagcg aagttcaata gagtttgagc gtagcgaaac atagggcaat
    10921 ttttcattca ttgggctttt aattattttt aatcttttaa atgcttttaa ataagctgta
    10981 aacctttatt tataatagtt tcatagttaa taaaactagg tttatcgacc cataaaacta
    11041 ggtttatcga cccataaaac taggtttatc gacccataaa actaggttta tcgaccttaa
    11101 agctaggttt atcgacccat aattaagtta atttacaact caattgaaat agtctattgt
    11161 acaacctagt ttaattataa atttattatt agaa
//
