LOCUS       NZ_CP040086            11194 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter baumannii strain VB33071 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP040086
VERSION     NZ_CP040086.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11571816
            Assembly: GCF_005280695.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Veeraraghavan,B., Vijayakumar,S., Vasudevan,K., Narasimman,V. and
            Venkatesan,M.
  TITLE     Carbapenem resistant Acinetobacter baumannii_VB33071_hybrid assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11194)
  AUTHORS   Veeraraghavan,B., Vijayakumar,S., Vasudevan,K., Narasimman,V. and
            Venkatesan,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2019) Clinical Microbiology, Christian Medical
            College, Ida Scudder road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.8; Unicycler hybrid v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 29.0x
            Sequencing Technology :: IonTorrent; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 01:33:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,844
            CDSs (total)                      :: 3,766
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 78
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 96 of 152
            Pseudo Genes (incomplete)         :: 55 of 152
            Pseudo Genes (internal stop)      :: 16 of 152
            Pseudo Genes (multiple problems)  :: 13 of 152
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040086.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11194
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="VB33071"
                     /isolation_source="blood"
                     /host="Homosapiens"
                     /db_xref="taxon:470"
                     /plasmid="unnamed2"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2018"
                     /collected_by="Christian Medical College"
     gene            join(9411..11194,1..628)
                     /gene="znuD2"
                     /locus_tag="FDN00_RS19235"
                     /old_locus_tag="FDN00_19235"
     CDS             join(9411..11194,1..628)
                     /gene="znuD2"
                     /locus_tag="FDN00_RS19235"
                     /old_locus_tag="FDN00_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            933..1394
                     /locus_tag="FDN00_RS19240"
                     /old_locus_tag="FDN00_19240"
     CDS             933..1394
                     /locus_tag="FDN00_RS19240"
                     /old_locus_tag="FDN00_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(1720..1929)
                     /locus_tag="FDN00_RS19245"
                     /old_locus_tag="FDN00_19245"
     CDS             complement(1720..1929)
                     /locus_tag="FDN00_RS19245"
                     /old_locus_tag="FDN00_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069474.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCEYFS
                     HCKRPSYYIDIFDNAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(1922..2224)
                     /locus_tag="FDN00_RS19250"
                     /old_locus_tag="FDN00_19250"
     CDS             complement(1922..2224)
                     /locus_tag="FDN00_RS19250"
                     /old_locus_tag="FDN00_19250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001129974.1"
                     /translation="MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2217..2573)
                     /locus_tag="FDN00_RS19255"
                     /old_locus_tag="FDN00_19255"
     CDS             complement(2217..2573)
                     /locus_tag="FDN00_RS19255"
                     /old_locus_tag="FDN00_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269904.1"
                     /translation="MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSNKKRFYTEMLAIADEQ
                     YALHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3139..3438)
                     /locus_tag="FDN00_RS19260"
                     /old_locus_tag="FDN00_19260"
     CDS             complement(3139..3438)
                     /locus_tag="FDN00_RS19260"
                     /old_locus_tag="FDN00_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            complement(3648..4166)
                     /locus_tag="FDN00_RS19265"
                     /old_locus_tag="FDN00_19265"
     CDS             complement(3648..4166)
                     /locus_tag="FDN00_RS19265"
                     /old_locus_tag="FDN00_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000839337.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPVQKQSVKETVKVDSPTSAEVLLLRQQIDLLKNQLDDAKNRESFYQNQIETMQRLL
                     EAPKTNTTQSDDPGSTQAESTVEEKEDVIAMPPKESEVIENKRIPVPEHIEPEPEKRGF
                     WSRFFKPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(4244..5179)
                     /gene="repM"
                     /locus_tag="FDN00_RS19270"
                     /old_locus_tag="FDN00_19270"
     CDS             complement(4244..5179)
                     /gene="repM"
                     /locus_tag="FDN00_RS19270"
                     /old_locus_tag="FDN00_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001292329.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLREASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YESQSGIIFLRFTQDIVPLITRLEENFTKYELQQVSRLSSSYAIRLYELLIQWRSAGKT
                     PLFDLSIFRQQLGVKPHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFTFKQKKMSNLPIKNKRDPDTIDIFSKMTDAQRHLFSHKLSELPEMGKYSHGTESYPQ
                     FAVRIAEMLQNPEKFKELYPYLQKVGFKAA"
                     /name="repM"
     gene            complement(5776..6945)
                     /locus_tag="FDN00_RS19275"
                     /old_locus_tag="FDN00_19275"
     CDS             complement(5776..6945)
                     /locus_tag="FDN00_RS19275"
                     /old_locus_tag="FDN00_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002134206.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIDFWQAADTYERANAKAYMEYEIALPNEFTSEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHEDYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENNPFSSLEVMYYLNYLIKNATNKDKLKNDLNEIIKNQGPILKQEAWSIYEMNEKLEDS
                     RRNSTPCFEFEVHSLPEYVLPIQNTPPKRTQSEPVQPKADDYDYGGPGF"
                     /name="MobA/MobL family protein"
     gene            7250..8011
                     /locus_tag="FDN00_RS19280"
                     /old_locus_tag="FDN00_19280"
     CDS             7250..8011
                     /locus_tag="FDN00_RS19280"
                     /old_locus_tag="FDN00_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425105.1"
                     /translation="MEMEKAKKRGRPAQLLQIAELHAFVEFLEQQEQLSDLQSQVLKAL
                     NSVDCNFEGLTQTDQVLVKEALKPYREHLKLKLLFEELNNLPLKTEYEQKFLDLYELFQ
                     KNALDQMELNILKTLATRYLNFKAQKLEYSDLELYLSQLQKKDAGKKRKAENQRKFELG
                     GAVLVAFKKLNIDISNDTPQQITNRIVNTTKFHNEVRKSLIFKDVKTYENEYFKANKLF
                     IQVLEGLHTWQKGGELLSVIEIKKALEKGEE"
                     /name="hypothetical protein"
     gene            8128..8427
                     /locus_tag="FDN00_RS19285"
                     /old_locus_tag="FDN00_19285"
     CDS             8128..8427
                     /locus_tag="FDN00_RS19285"
                     /old_locus_tag="FDN00_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            8695..8982
                     /locus_tag="FDN00_RS19290"
                     /old_locus_tag="FDN00_19290"
     CDS             8695..8982
                     /locus_tag="FDN00_RS19290"
                     /old_locus_tag="FDN00_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438827.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            8969..9283
                     /locus_tag="FDN00_RS19295"
                     /old_locus_tag="FDN00_19295"
     CDS             8969..9283
                     /locus_tag="FDN00_RS19295"
                     /old_locus_tag="FDN00_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     Typing          complement(4245..5179)
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5780..6867)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggatgattta cttgattatc aaatcagtac ttactattat gattttgata actatatata
       61 tttacagaca ctcaatgaag ttttaggaac tacaaaagtt agagatcagc atacactacg
      121 tattaatcat tatagccagt ctgctgctaa tttttatgga ttagagggca acatagggta
      181 tcaatttaat tctgtttatc acggctctct ttttggagat tatgtgaaag gccgtttaac
      241 aaatttacct gatgctgtaa ttgcatacga tatctggaat agagaaccga ctttagctcc
      301 ccaaaaggat cgttacactc ctagattacc tcctgctcgt ttgggtacac gactaaaagc
      361 tgattttgat gaatcgctta aaggtgaaat agaatattat cgagtcttta aacaagataa
      421 tatttctaaa tttgagcagg ttacatcagg ttataatatg cttaatatga ctttagcata
      481 taaaaataag ctatctcaca cagaatatga tttattcttt aaagcgaata atttacttga
      541 tcagaaagtc tatgcccatg aaacattttt gccatatatt ccccaaattg gaagaaattt
      601 tagtttaggc ttaaatctta atttctagat aagatactgt aatcttctaa gaaaatttct
      661 tagaagattt ttcttataaa atgattagat taataattaa ggtaagcgtc cgctgaatcc
      721 catacctatt ttttaattcg atttaggttt ccagcagtgt ggcagtaact cttcaatctg
      781 ggtcacttta tgtgtcggca gccttttcag cactgaatag ccaccgattt tgtagacacc
      841 ttttagctag ataaaaagca atttttttga tctttaaaga atgaggatat aagctgttta
      901 aaacttaatt ctaattggtt aataggtgaa aaatgaaatt agcagaagct ctcttacttc
      961 gaagtgatca acaaaaaaaa attatttcac taaaacaacg tatcaatgca aatgtattgg
     1021 tacaagatgg tgatcaacca tctgaagatc caaatgaact actaaaacaa gtattttctt
     1081 taattcaaga atttcaaaaa ttaagttatg caattcatga aactaatgct ttaacaaaat
     1141 taaatgatgg ccgttctcta cttgctttat tgactttgcg tgatgaattt gtagaacagc
     1201 acaaaaccct aactgcagca ataagtaata catctcgtga atctgatcgt tatagtacgc
     1261 gggaaataaa gtggcataaa gttattccag tttcatcatt acaaaaacaa gctgatgata
     1321 ttagtcttaa attacgtgat ttaaacgtgt taattcagtc gactaattgg aaaattgatt
     1381 tgattgaagc ctaatagtcc tttagatata atcaagttga acatactggg cgagtacaag
     1441 atcccttact tccacaacat aggattgtaa ataaagtgta gtggcttgca aagcaagtgg
     1501 ataagcttaa aattttaagt acaaatcaat ccatcacttg gcataactca aatctttgtg
     1561 caaacaaagt tacacgttac ttataactac catgtactga cagtatgttc acctcaatta
     1621 ctaaaaataa agaaatgagc aagcatcagc tcaactacat ttctattttt aattcatttc
     1681 ggcatttaac ataaaatctc ttatgcgaat ctagaaggat taagctattt cgtaatattt
     1741 tatccaataa gcattgtcaa aaatatcaat gtaatagctt ggcctcttac aatgtgaaaa
     1801 atattcacag cataaatctc taataatttc aatttcttct aggctatcgg cagcattgac
     1861 taaaaatata aacttgtcta agttaacaag taatttagta ctatttttca aaggtttatt
     1921 attagacatt gaaccctatg tgttttcctg ttggaagctc tacatcaata cgaagttttc
     1981 cacccattgc ttcaacatac tttttcatcg ttgaaatttt aagatcatga ccacgatttt
     2041 cgattgctga taatgacggc tgtttaattc caagggttgc agctagttct ttctgggaaa
     2101 tttctaactc ttctcggata aggtgaagct gactttctaa aagtaattca tcagccattt
     2161 tttgaatacg agcttgactc tctggagagc gactagccaa taattcttgt aagctcttag
     2221 ccattagatt gatctcctaa agttgagaga tgaagtgcgt attgctcatc tgcaattgct
     2281 agcatctctg tataaaaacg ctttttatta ctcttatcac caatacacag cacaatcgct
     2341 tgtcttaggg gatcgaatgc aaaaaatgcc cttaacggtt taccacgatg ctgaacacgt
     2401 agttccttca tattggtaaa tttagaatcg tagacggtat ctactaaagg acgacctaaa
     2461 ctaggccctt gctgttgtag aacaaccaat gcagctaaga ctttctcttg tgttgcttca
     2521 tcttgctgtt caagccattc attaaacaga tccgttgtaa tgactgtcca catattacaa
     2581 tcacaatata gattacagtc tatatattaa atctgattga atgactttgc aaatggcaat
     2641 aaaaaatccc gacgtcatgt cgggattttt cggtatggag cgcataggta gggctgacta
     2701 cctgatggtc atggtgcctg actgattatt attgttgttt taagtcattg aacatcctgt
     2761 ccatatagat cgatcataag agcttcatcg ggggatagat acagacaatt tcgtgaaacg
     2821 ctgtaagcca aaacgtacaa aaaataatca aaatttatcc cagtggttat ccacagaaat
     2881 agtggattct tttgggtaaa aaagcttata aactctatgg aattgctatt tttgattatt
     2941 ttttaaacaa ttttattggc aagaagtatc aaaattgagc ataaaaaaac cgacttgttt
     3001 ctaagtcggt tgaacgaggt cgttccagag ggtatgactc aatgtaagct actgatctta
     3061 aagtaatctt aaagatccat ttcgcataat atccattatg ttaaatagta ggggactcta
     3121 agtacaagat tagaaaattt agtctttaag aggagaaatt tgttctagct tatcttcagg
     3181 attattttct ctaatatgta taattcgatc ataattttct actcctcgta agtgaggtgg
     3241 tgcaaatgct ggatacttgt tgaaaaagtg cattttaata tttagtgcat ggtctaaaat
     3301 aaaagcacat tcaattccat agtacttatc attaataaaa aaattgtctg tagtttctat
     3361 tagttcttga atagctaaat ccaatttttg aattttttca ttaatatctt tagttttctc
     3421 ttctatcttt ttgtccattg gtatcaccta tagaattaag tttccctaaa attaacataa
     3481 tacaccttat acgaaattct ttgtaaaaag cattttaaaa caatgtctta tggatgtaaa
     3541 aacagcctaa gcagtcacgc tcaggctgtt ttagtgattt tttatgtttc acgatcaatt
     3601 attgatcatt gttccacggt tttgattagt ctgattaaat agtcctatca tccatagggc
     3661 ttgaaaaaac ggctccagaa gccccttttt tctggttctg gctcaatatg ttcagggact
     3721 ggaatacgct tattctcgat aacctctgac tccttaggag gcatagctat gacgtcctct
     3781 ttttcttcta ctgtggattc tgcctgtgtt gatccaggat catcagattg tgttgtattg
     3841 gtttttggag cttccaatag gcgttgcata gtttcaattt gattttgata gaagctttct
     3901 ctattttttg catcatctag ctgatttttt agtaggtcta tctgctgtct aaggagtaaa
     3961 acttctgccg aagttggact gtctactttt acagtttctt ttacactctg tttttgtact
     4021 ggctcaccaa atacccggat cgcttctgca agatcaatct tattgtcaga atttttactt
     4081 aaaatacctt tctttatatt gttgtaaatg gtttgtcgat taatattgta aagcttagaa
     4141 agctcaagaa ctgtaaggct tttcatgtgt aaatctctgc ctaatcttgt aaaaaattag
     4201 tgtctattag actgtctaat agacaaatca gcaagtctat cttctatgca gctttgaagc
     4261 ctactttttg gagataagga tagagttctt taaacttttc tgggttttga agcatttctg
     4321 caatgcgtac agcgaattgc ggatagcttt ctgtaccatg agaatacttt cccatttcag
     4381 gaagttctga cagtttgtgg gaaaacagat ggcgttgagc atctgtcatt tttgagaaaa
     4441 tatctatagt gtctgggtca cgcttatttt ttattggcag attactcatt tttttctgtt
     4501 tgaaagtgaa tgaaaaacct gaaatagaac gccctttttt atgttgctca tatttagctg
     4561 ttatatcggt taactcattt acctgcttaa gagcaaaatc taaaacatat gttttaaagt
     4621 tactcattgt tttgtattga tgaggtttga caccaagttg ttgtctaaag atagatagat
     4681 caaaaagtgg cgtttttccg gcagatctcc attgaattaa tagctcgtat agccgaatag
     4741 catatgagct acttaaccta gaaacctgct gcaattcata tttagtgaaa ttttcttcaa
     4801 gtcgagttat gagtggaacg atatcttggg tgaatcgtag gaaaataatt cctgattgag
     4861 attcataccc aattttgtcg acccaacgac aatgaaagct acgatcctta cctgtcttag
     4921 gattaatgtc atggtatgtg acatagcgat caaataagct tttagatgcc tctctgagta
     4981 cggtataagc ggtatgtttc tcgacattaa aagtatttat ataactactt gcatgtactt
     5041 ctaaaagact attttcagtt atcccatgtc cagtttctcg agcttcagca atggctaata
     5101 ggatcagtct ttgttcaact gtatctaagg tataacttgc ttgaattaga gcattatcct
     5161 ttacgattaa ttcactcatt tctaataata aatttataat taaactaggt tgtacaatag
     5221 actatttcaa ttgagttgta aattaactta attatgggtc gataaaccta gctttaaggt
     5281 cgataaacct agttttatgg gtcgataaac ctagttttat gggtcgataa acctagtttt
     5341 atgggtcgat aaacctagtt ttattaacta tgaaactatt ataaataaag gtttacagct
     5401 tatttaaaag catttaaaag attaaaaata attaaaagcc caatgaatga aaaattgccc
     5461 tatgttttcg ctacgctcaa actctattga acttcgctta cgctcagttc gaaggggcaa
     5521 tttttgggtt aatctcgtga taacttcaaa aaagcaaaag caactcggaa ttcgcttcgc
     5581 tcataagttt tttatactcg cttcgctcga cctagatgca aattaaatct gttcgcaccc
     5641 tccgggatgc tctgtatctc agtctcagca gcaaagggga gtttgtaatc agggaaaaag
     5701 catggcaaaa attttaattg tgtctgttcg ctcgtagaca ctcgctctgt aaactttgga
     5761 taaaaccaac tgagcttaaa aaccagggcc accgtaatca taatcatcag ctttaggttg
     5821 tactggttct gattgagtgc gtttgggagg cgtattttgt atcggtaata cgtattcagg
     5881 aagtgaatgc acctcgaact caaaacatgg ggtactgtta cgtcttgagt cttctagttt
     5941 ttcattcatt tcgtagatgg accaagcttc ttgtttaagt atagggcctt ggtttttgat
     6001 aatttcattc aaatcatttt ttagtttgtc cttatttgtt gcattcttga ttaagtaatt
     6061 caggtaatac atgacttcca atgatgaaaa tgggttgttt tcagcactat aaaaatattc
     6121 atgttgttga tctaattgct tttggcgttc ttcgagttct cgttcttgct ggttgtgttc
     6181 tctgattttt tggatttggg cgagatattc cgctgtcgca ttttgttgtc caacgcctag
     6241 ttttggctct ggaaggtata gcgtgctgtg gatctcgtta tatttttcat agctgtctgc
     6301 tgaaacctga tttttgatgg tgatgtcttg gccatccagt gtgtaggtga cggtcgggca
     6361 gtgctgtgca agggcatcat tcaaatgatt ttcccattgt tttcggatat agatcaaaaa
     6421 agtggaatag tcttcatggc catcttgaag ttggattttt ttggcaccgc ctttggctgg
     6481 atcttgaggg ttatagcgtt tgaagtactg ttctgcgctg cgttcaatgc cgtcatccgc
     6541 ttttagcgag aacatcagat gacaatgagg ctgatcttcg ccactgatac gtgattttac
     6601 gttgtgtatg gcgtatgagt gcggatattg ctgtggaact atatgtttgt ctaaaaacgt
     6661 ctctatgagc gttttacgct gttctgacgt gaactcattc ggtagggcaa tttcatactc
     6721 catgtaggcc ttggcattgg ctcgttcata cgtatctgct gcctgccaaa aatcaatggg
     6781 atcttttaca aaggcaggca tacatcgacc aggttcaata tgttctatgg tctcattgct
     6841 ggttttgcgg atgccttcat actgcatcag ccgggaaatg tagagataat ggccggtcgc
     6901 atagccctta tttttgcttt tgacgctgaa gtggtagctg gccatgttca cctctgctgg
     6961 gttgtgggtt cggtttttat gcagtgaaac ggaatgaaaa ccgcccggat ttgaaatgcc
     7021 tgtatggctg aaaaatccgg ttgaaggggg tcaaggggga agcgccccat tgcgtactag
     7081 tttctccgat gaggagaaac tgtcaagtgc gaattattta atataaaaat aaaatttctt
     7141 gaaaagaatt tttattttta tattaaataa ttattttcag actgccctct ttaaatatgg
     7201 taaaaccata tttaaagagg ttgcagttct gaaaatttgg cggcagatga tggaaatgga
     7261 aaaagcaaaa aagcgtggtc gacctgctca gttgttacag attgctgagt tacatgcatt
     7321 tgtagagttt ttagaacagc aagaacagct ttctgatctt caatctcaag tgttaaaggc
     7381 tttaaatagt gttgattgta attttgaggg gctaactcag acagatcagg ttctggtcaa
     7441 agaggcttta aaaccttatc gtgaacatct gaagttgaaa ttgctgtttg aagagttgaa
     7501 taatctgcct cttaaaaccg agtatgaaca gaagttttta gatctgtatg aactttttca
     7561 aaagaatgca ctagatcaga tggaactaaa tattttaaaa accttggcga cacggtatct
     7621 gaattttaaa gcgcaaaagc ttgaatacag tgatttagaa ttgtatttga gtcagctaca
     7681 gaagaaggat gctgggaaaa aacgcaaagc tgaaaatcaa cgtaaatttg aattaggtgg
     7741 tgctgttctt gtcgcattta agaaactcaa tattgatatt agcaatgata ctcctcaaca
     7801 gattaccaat cggattgtga atacgacaaa gtttcataat gaagtacgta aatctttaat
     7861 ttttaaagat gttaagacat atgaaaatga atattttaaa gcaaataaac tttttattca
     7921 ggtgttagaa ggattacata cctggcaaaa gggcggagaa ttattgtcag taattgaaat
     7981 taaaaaggcc ttagaaaaag gcgaagagta gtttatttag cagggatttt agagaagttg
     8041 gacatttcta ttttgggctt acattggaat ttcgcataag gtgtattatg ttaattttag
     8101 ggaaacttaa ttctataggt gataccaatg gacaaaaaga tagaagagaa aactaaagat
     8161 attaatgaaa aaattcaaaa attggattta gctattcaag aactaataga aactacagac
     8221 aattttttta ttaatgataa gtactatgga attgaatgtg cttttatttt agaccatgca
     8281 ctaaatatta aaatgcactt tttcaacaag tatccagcat ttgcaccacc tcacttacga
     8341 ggagtagaaa attatgatcg aattatacat attagagaaa ataatcctga agataagcta
     8401 gaacaaattt ctcctcttaa agactaaatt ttctaatctt gtacttagag tcccctacta
     8461 tttaacataa tggatattat acgaagtaca cttgtatatt aatttaaata tcaactactt
     8521 aataaaaata taaaaatcca aaaatgccca aaaagccgac tttgaaacaa gtcggctttt
     8581 ttatgatcga ttttgatagt ttttgccaat aaatctgact aaaaattacg cttctcaact
     8641 tgagtgatac aatttcgtga tacattattg tatatacaaa tttgaatatt ttttatggaa
     8701 cagtattttg aatgggatga ggctaagaat cgaaagaatc agaaaaagca tgatatctct
     8761 ttcgaaactg caagccttgt ttttgaagat ccattacgga tctcaatcca agacagatat
     8821 actaatggtg aagaacgttg gcaaaccatt ggacgagtta aaggcgtact aatgttattg
     8881 gtggctcaca ccatttttga tgaagatgac tgtgaaatca tccgaatcat tagtgcaagg
     8941 caagtcacta aagcggagcg aaacaaatat gagcatggtt agatactcac acaaagagct
     9001 gaatgagaaa tttggtgaga agcaagatgc tgaaattcaa cgcttgcttg ctaaaggcac
     9061 ggttcctgat gatcagctag atctatcaga tattcctgaa attacagact ggagcaatgc
     9121 tgtacgccaa aatcaatttt accgcccagt aaagcaacaa acttcaattc ggttagatgc
     9181 tgatgtactt gcttggttca aagcacaagg caaaggttat caaacacgta tgaatgaaat
     9241 tttacgagat gctatgctaa aagaattaaa aaatcatcaa taagaatggg agcttaggct
     9301 cccatttttg acttcatttt taggggggat taccattcaa aaatttaaat agtagatttt
     9361 ataaattttt aagtgttata ttataatata acattaaatt tggttgtttt atgctaaata
     9421 aaagtaaact atttttagct ttaattactt taggggcctc gaaaatttta ttggcagctg
     9481 aagggcctgt tactacctta aatacaatag tattaactgc acaatctgat gagttagggt
     9541 ctgagctgtt aggcaaaagt ctaaatgtta gtaatcagtt tattgatact agtaaactta
     9601 aacagcgttc gacaactcta ggggatgcct tgggtacgga attaggaatt cattctaatc
     9661 aatatggagg tggagcatct actcctatta ttcgtgggca ggaaggaaaa cgtattaaag
     9721 ttttacagaa taatgctgac gtgttggata tgtctaatat gtctccagat cacgctgtca
     9781 cagttgaacc aagtttagct aagtctattg aaattatacg tggtgcaagt acccttttgt
     9841 atagttcaaa tagtgcggca ggggtagtca atgtcattga ctataaaatt ccaacccaaa
     9901 tgcctcaaga cggattagag ggtaatacta cattacgttt taatactggc agtaatgaga
     9961 agttgaccac agcaggtgta acagttggct taagcccaca tgtagcttta cgggcagaag
    10021 ggttatatcg taatgctggt aactataaaa ctcctcatta ccagtctagc tcttataatt
    10081 ctttagaaga tcttgaaaac caaaacagta tttataaaaa tttaaaatat ttaccagaaa
    10141 gctgggcaga gtcgagagta ggtacgcttg gattatcctg gatagacgat aatacttatt
    10201 taggagtttc ttatacacat cgtcatgatg aatatggttt accagcccat agccatttat
    10261 atgaaggttg tggtgcctct gcaatcggta tcgattcacg aattagtgga ttaaaaaatt
    10321 atcttttata ttatcctcaa ttgatggatg agcaagatat taattacata aatccacgtc
    10381 ctgattgtca tcagcataat catattcatg aaactaattt ctctcataat gctccttata
    10441 ttgatttaaa tactcgacgt tatgatgtgc gtggagaatt cactcaacca tttaccggta
    10501 ttgacaaaat tcgtacctca ttgagttata tagattattt tcacaatgaa ctcgagggag
    10561 ataagattac taactttttt aaaaatacag gaaaagtagg ccgcatagag ttatcacatc
    10621 agccattagg ggaattaacc ggtatcttgg ggctacaata tttggaacag gataatagtg
    10681 ctttatcacc agtccattct caagaaggcc ataccaccta tctagataat cagcagctat
    10741 taaatcgaaa tgtcactaaa aactttagtg tatttggcct agaaaaatat aactggaacg
    10801 actttacttt cgaactaggt gctcgtatag aaaagcagaa agttagtatg gattatgata
    10861 tagaaaaaat taaagattca atgaaacctt ggcctaataa atataacagt ccttatgtag
    10921 aaaaaaataa taaaatcagg gctcaaaatt taaaatctat tttagaagct gttcaaccta
    10981 acaaagaaac agctttttct tatgcaggta cagtccactg gagatttgct ccaaattata
    11041 tcttatcatt aacaggtaca catcaagaaa gattacctaa tgcacaagaa atgtatactc
    11101 atggtatgca tctcgctact aattcttttg aaataggtaa ccgctttcta agaaaagaga
    11161 aatccaataa tttagaaatc agtctggcct ataa
//
