LOCUS       NZ_CP040076              763 bp    DNA     linear   CON 20-JUN-2024
DEFINITION  Escherichia coli strain A1_181 plasmid p_unnamed7.3.
ACCESSION   NZ_CP040076
VERSION     NZ_CP040076.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10887807
            Assembly: GCF_006352265.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Ahlstrom,C.A., Ramey,A.M., Woksepp,H. and Bonnedahl,J.
  TITLE     Repeated detection of carbapenemase-producing Escherichia coli in
            gulls inhabiting Alaska, USA
  JOURNAL   Antimicrob. Agents Chemother. (2019) In press
  REMARK    Publication Status: Available-Online prior to print DOI:
            10.1128/AAC.00758-19
REFERENCE   2  (bases 1 to 763)
  AUTHORS   Ahlstrom,C.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2019) Alaska Science Center, USGS, 4210 University
            Drive, Anchorage, AK 99508, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. NOV-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 127.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_006352265.1-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 02:46:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,130
            CDSs (total)                      :: 5,006
            Genes (coding)                    :: 4,741
            CDSs (with protein)               :: 4,741
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 265
            CDSs (without protein)            :: 265
            Pseudo Genes (ambiguous residues) :: 0 of 265
            Pseudo Genes (frameshifted)       :: 80 of 265
            Pseudo Genes (incomplete)         :: 175 of 265
            Pseudo Genes (internal stop)      :: 50 of 265
            Pseudo Genes (multiple problems)  :: 36 of 265
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040076.1.
            Bacteria and source DNA available from USGS Alaska Science Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="A1_181"
                     /isolation_source="feces"
                     /host="Larus sp."
                     /db_xref="taxon:562"
                     /plasmid="p_unnamed7.3"
                     /geo_loc_name="USA: Alaska, Anchorage mudflats"
                     /collection_date="2016-08-09"
     gene            <1..702
                     /gene="mobV"
                     /locus_tag="FDP43_RS25910"
                     /old_locus_tag="FDP43_25920"
     CDS             <1..702
                     /gene="mobV"
                     /locus_tag="FDP43_RS25910"
                     /old_locus_tag="FDP43_25920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_077108195.1"
                     /translation="RQQAEFFARSEQWLEKKYGKDRVVAAVVHRDEATPHLSAFVVPLT
                     QDGRLSAKEFIGGRSKMREDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTR
                     SQVSISPEALEPRVLRKGIFTKDVEDQAAIAKRLSHAVNDGFAGTIAMASQSAQNAKRA
                     RELQKTMDAQQKRLQSVTEPFKGLSREQMTEILMMAQRFKQQNQEKEKQQRIEREKQRQ
                     TRSRGMGGMER"
                     /name="mobV"
ORIGIN
        1 cggcagcagg cggagttttt cgcccgctct gagcaatggc tggagaagaa atacgggaag
       61 gatcgtgttg tggcggcggt ggttcaccgg gacgaggcca ccccgcacct gtctgcgttc
      121 gtggtgccat tgacgcagga tggacggctg tccgcgaaag agtttattgg cgggcggtca
      181 aagatgcgtg aagatcagag cacctacgct gaatcagtaa aaaaactggg tcttgagcgc
      241 ggcatcgagg gaagccgcgc cacgcatcag acagttcagc actactacga atcgataaac
      301 cgtggcaccc gtagccaggt atcgatctcg ccggaggcgc tggagccgcg tgtattgcgg
      361 aagggcattt tcaccaaaga cgtggaagat caggcagcca tagccaaacg tctaagccac
      421 gcagtgaacg atggctttgc aggaaccatc gctatggcct ctcagagcgc ccagaacgca
      481 aaaagagcgc gtgagctaca gaagactatg gatgcgcagc agaaacgcct acagagcgtt
      541 acagagcctt ttaaggggct ttccagagag cagatgaccg aaatcctgat gatggctcag
      601 cggtttaagc agcaaaacca ggaaaaggaa aaacagcagc gtattgaacg tgaaaaacag
      661 cgacagacgc gatcgcgtgg catgggtggt atggagcgtt agttttgatg cgatgcagga
      721 tagggctaac gccgcaaagt gacgtcattt gcggcattcc aga
//
