LOCUS       NZ_CP040075             1265 bp    DNA     linear   CON 20-JUN-2024
DEFINITION  Escherichia coli strain A1_181 plasmid p_unnamed7.2.
ACCESSION   NZ_CP040075
VERSION     NZ_CP040075.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10887807
            Assembly: GCF_006352265.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Ahlstrom,C.A., Ramey,A.M., Woksepp,H. and Bonnedahl,J.
  TITLE     Repeated detection of carbapenemase-producing Escherichia coli in
            gulls inhabiting Alaska, USA
  JOURNAL   Antimicrob. Agents Chemother. (2019) In press
  REMARK    Publication Status: Available-Online prior to print DOI:
            10.1128/AAC.00758-19
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Ahlstrom,C.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2019) Alaska Science Center, USGS, 4210 University
            Drive, Anchorage, AK 99508, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. NOV-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 127.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_006352265.1-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 02:46:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,130
            CDSs (total)                      :: 5,006
            Genes (coding)                    :: 4,741
            CDSs (with protein)               :: 4,741
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 265
            CDSs (without protein)            :: 265
            Pseudo Genes (ambiguous residues) :: 0 of 265
            Pseudo Genes (frameshifted)       :: 80 of 265
            Pseudo Genes (incomplete)         :: 175 of 265
            Pseudo Genes (internal stop)      :: 50 of 265
            Pseudo Genes (multiple problems)  :: 36 of 265
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040075.1.
            Bacteria and source DNA available from USGS Alaska Science Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="A1_181"
                     /isolation_source="feces"
                     /host="Larus sp."
                     /db_xref="taxon:562"
                     /plasmid="p_unnamed7.2"
                     /geo_loc_name="USA: Alaska, Anchorage mudflats"
                     /collection_date="2016-08-09"
     gene            complement(3..443)
                     /locus_tag="FDP43_RS25900"
                     /old_locus_tag="FDP43_25910"
     CDS             complement(3..443)
                     /locus_tag="FDP43_RS25900"
                     /old_locus_tag="FDP43_25910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_024192735.1"
                     /translation="MSKDTQRITQINDQGEIVGGFVAVIRPKQKSSFQRHFTMNQDALK
                     ILAKELTGEQFKVLMLMLADLDYENFIQIAQADIADALEMQKTHVSRAVRALLDVGVIF
                     EGPKVGRSKTYRLNEQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="MarR family transcriptional regulator"
     gene            999..>1265
                     /locus_tag="FDP43_RS25905"
                     /old_locus_tag="FDP43_25915"
     CDS             999..>1265
                     /locus_tag="FDP43_RS25905"
                     /old_locus_tag="FDP43_25915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_172605386.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATP"
                     /name="plasmid recombination protein"
ORIGIN
        1 gatcaggttc tgccgccctg tataacgctg aggccgtttt taagggcttt tttgtgattg
       61 cttactgttc ctttccagcc gaattgctcg ttcaaccgat aggttttgga tctgcctact
      121 ttcggacctt caaagatcac gcctacatca agaagagcac gaacagcgcg gcttacgtgg
      181 gtcttttgca tctctaatgc atccgctata tcagcctgtg caatttggat gaaattctcg
      241 taatccagat cagcgagcat gagcattaga actttaaatt gctcgcccgt tagctcctta
      301 gcgaggatct ttaatgcgtc ttggttcata gtgaaatgcc tctgaaatga cgatttttgc
      361 ttgggtctga ttaccgcgac gaagccgcca acaatctcac cttgatcgtt aatctgggtg
      421 attctctggg tgtccttgct catcgaaaat aacctatctt gagttaccaa taatggtaac
      481 agtgttacca ttattggtaa ctatgtcaaa tgtaacccct cgccatacat acgttttttt
      541 atcgctcctt ctgtatctta taacgcgtgc gcgcggttta gctctgaata ccaacgtttt
      601 ttagctacgg agtgtacgga tttctatgta cggggagtgg aaattccgct tcctcgctca
      661 ttcgctcact acgttcggtc atgagtctgc ggcgagcggt tcctgctctt tcaaaagcga
      721 tattcattgc ctgatttcgt caagggcgag cgaagcgagt gccatttacc cttgacgaca
      781 ctccggataa aacaggcttg gcagtggtgg ggggggcgcg gagcgacgcc ttccacctgc
      841 cagaccgccg ttgattttga ccttctggca gcttgctgcc cgaacgattt tgcgaagcaa
      901 agtctagtga gtacactatc atcttgatag tggcccgccg gaggtatcgc aatgcgctcc
      961 cccgcctggc tgaatgaatg ccggaatcag gggggaacat ggcgaactac gcgataatgc
     1021 gctgcaaaaa gctgacggga atgggaagcg tggccagcgc gttgcagcac tgctaccgag
     1081 agcgtgagac gccgaacgct aacgctgaac ggacaccaga aaactactgc tctgtgtcca
     1141 aatcaacgga tcaggcaatg gggcgtgtgc gggagctgct gccggaaaaa cgccgtaaag
     1201 atgcggtact ggccgttgaa tacgttatga cagcaagccc tgagtggtgg aaagaggcca
     1261 cacca
//
