LOCUS       NZ_CP040073             1806 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli strain A1_181 plasmid p_unnamed6, complete
            sequence.
ACCESSION   NZ_CP040073
VERSION     NZ_CP040073.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10887807
            Assembly: GCF_006352265.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ahlstrom,C.A., Ramey,A.M., Woksepp,H. and Bonnedahl,J.
  TITLE     Repeated detection of carbapenemase-producing Escherichia coli in
            gulls inhabiting Alaska, USA
  JOURNAL   Antimicrob. Agents Chemother. (2019) In press
  REMARK    Publication Status: Available-Online prior to print DOI:
            10.1128/AAC.00758-19
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Ahlstrom,C.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2019) Alaska Science Center, USGS, 4210 University
            Drive, Anchorage, AK 99508, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. NOV-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 127.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_006352265.1-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 02:46:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,130
            CDSs (total)                      :: 5,006
            Genes (coding)                    :: 4,741
            CDSs (with protein)               :: 4,741
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 265
            CDSs (without protein)            :: 265
            Pseudo Genes (ambiguous residues) :: 0 of 265
            Pseudo Genes (frameshifted)       :: 80 of 265
            Pseudo Genes (incomplete)         :: 175 of 265
            Pseudo Genes (internal stop)      :: 50 of 265
            Pseudo Genes (multiple problems)  :: 36 of 265
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040073.1.
            Bacteria and source DNA available from USGS Alaska Science Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="A1_181"
                     /isolation_source="feces"
                     /host="Larus sp."
                     /db_xref="taxon:562"
                     /plasmid="p_unnamed6"
                     /geo_loc_name="USA: Alaska, Anchorage mudflats"
                     /collection_date="2016-08-09"
     gene            926..1039
                     /locus_tag="FDP43_RS25875"
                     /old_locus_tag="FDP43_25885"
     CDS             926..1039
                     /locus_tag="FDP43_RS25875"
                     /old_locus_tag="FDP43_25885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_007772075.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHEKTPLAAAIGN"
                     /name="hypothetical protein"
     gene            complement(1500..1682)
                     /locus_tag="FDP43_RS25885"
                     /old_locus_tag="FDP43_25895"
     CDS             complement(1500..1682)
                     /locus_tag="FDP43_RS25885"
                     /old_locus_tag="FDP43_25895"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139972941.1"
                     /translation="MLTFIGVVIVAVIAFKLFMLFFSSSESAVERATRKYMENPTRANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     Typing          747..1257
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.359"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aacgcccctg atgatttctg accggctgca tggctatgca cagaagtgaa tggctggcag
       61 tttcacgcgt atgttgtcac taaggccgct ttttcgcagg aaacggtcat ttcctataac
      121 ggtgaacggg cgcgcagttt actgtgcgat cgtgaggcgt tgagcaacga gctttgcgag
      181 gcggtagggg ttcagagagc cagctgcagg ctcaggagaa aacattctgt tttcggggca
      241 aacgccgcaa atgacgtcat tttgcggcgt ttgccctatc ctgcatcgca gcatacggtg
      301 ccatccgcca ccctcttgcg aatcctgccc tagatctcaa cagcagagaa atttcgtacg
      361 tacgaaattc agcgtatcgg gcggcgcctg atgaaacatt ctggagtgtc ggcggtactc
      421 caaaaacaca tcttgtcccg ctgattttgt cactggcgca aagcggcggt aatgacaaga
      481 catttcctgt ccgcatcaat acgtgcagaa aacgtaagtg gtgaaagatg atagtacgga
      541 gcgacaggag cctaagcagc caccggagaa attacgcttc ctcggtcatt cgctcactgc
      601 gttcggtcat gactctgcgg cgagcgttac gggatcaatc aaagggcgtg gaggtatgaa
      661 gtgtgagaag agacttaagg gctgccggtt ttccataggc tccgcccccc tgacaagcat
      721 cacgaaatct gacgctcaaa tcagcggtgg cgaaacccga caggactata aagatcccag
      781 gcgtttcccc ctggtagctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
      841 gtcattccgc tgttatggcc gcgtttatct cattccacgc ctgacactca gttccgggta
      901 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc
      961 cttatccggt aactatcgtc ttgagtccaa cccggaaaga cacgaaaaaa cgccactggc
     1021 agcagccatt ggtaactgag atgtgcgaga gatttatctg gagttcttga agtggtggcc
     1081 tgactacggc tacactggaa ggacagattt ggtgacatgt ctcgcacaag actgttacca
     1141 cggttcagaa acttgccggt ttactgaacc ttcgaaaaac caccttgccg ggtggttttt
     1201 tcgttttcag agcaaaagat tacgactgaa acgacagaat cacaagaaga tcattaactc
     1261 cgagagtagt aactcccacc atgtttgcct atcaacacac ttaggaaaca ttacttgcgt
     1321 ttagttatac gacttctagc tctccataac agatcctcat tttctgctga tggatttcga
     1381 cggtatcgtt cctccgcttt ccgtactcca tattcaggaa acaccctgac agcaaccatt
     1441 tgtataaaga cataacacac caaaagtaaa aataaaaaac caataaactc gaacatatat
     1501 caccctcttt tcttctcatt tctcatgcgt gtagccacca ttattttata gttagctctc
     1561 gtcgggttct ccatatattt acgggtagca cgttcgacgg cagattcaga tgaactaaaa
     1621 aaaagcataa ataatttaaa tgcaataacg gcgactatca ctacaccaat aaatgtaagc
     1681 atcactctta ctcctgttat caacattgaa cataacatct cttgggcgca ccgcctgaga
     1741 gcctctcagc gcgtttctgc ggcccctgcc gattttcggc acctttcccc gcccataccc
     1801 ctgaaa
//
