LOCUS       NZ_CP040071             7406 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli strain A1_181 plasmid p_unnamed4, complete
            sequence.
ACCESSION   NZ_CP040071
VERSION     NZ_CP040071.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10887807
            Assembly: GCF_006352265.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Ahlstrom,C.A., Ramey,A.M., Woksepp,H. and Bonnedahl,J.
  TITLE     Repeated detection of carbapenemase-producing Escherichia coli in
            gulls inhabiting Alaska, USA
  JOURNAL   Antimicrob. Agents Chemother. (2019) In press
  REMARK    Publication Status: Available-Online prior to print DOI:
            10.1128/AAC.00758-19
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   Ahlstrom,C.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2019) Alaska Science Center, USGS, 4210 University
            Drive, Anchorage, AK 99508, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. NOV-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 127.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_006352265.1-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 02:46:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,130
            CDSs (total)                      :: 5,006
            Genes (coding)                    :: 4,741
            CDSs (with protein)               :: 4,741
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 265
            CDSs (without protein)            :: 265
            Pseudo Genes (ambiguous residues) :: 0 of 265
            Pseudo Genes (frameshifted)       :: 80 of 265
            Pseudo Genes (incomplete)         :: 175 of 265
            Pseudo Genes (internal stop)      :: 50 of 265
            Pseudo Genes (multiple problems)  :: 36 of 265
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040071.1.
            Bacteria and source DNA available from USGS Alaska Science Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="A1_181"
                     /isolation_source="feces"
                     /host="Larus sp."
                     /db_xref="taxon:562"
                     /plasmid="p_unnamed4"
                     /geo_loc_name="USA: Alaska, Anchorage mudflats"
                     /collection_date="2016-08-09"
     gene            1..840
                     /locus_tag="FDP43_RS25765"
                     /old_locus_tag="FDP43_25775"
     CDS             1..840
                     /locus_tag="FDP43_RS25765"
                     /old_locus_tag="FDP43_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            827..1678
                     /gene="repC"
                     /locus_tag="FDP43_RS25770"
                     /old_locus_tag="FDP43_25780"
     CDS             827..1678
                     /gene="repC"
                     /locus_tag="FDP43_RS25770"
                     /old_locus_tag="FDP43_25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            2033..2938
                     /gene="erm(42)"
                     /locus_tag="FDP43_RS25775"
                     /old_locus_tag="FDP43_25785"
     ARG             2034..2935
                     /name="erm(42)"
                     /gene="erm(42)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(42)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(3060..>3227)
                     /locus_tag="FDP43_RS25780"
                     /old_locus_tag="FDP43_25790"
                     /pseudo=""
     CDS             complement(3060..>3227)
                     /locus_tag="FDP43_RS25780"
                     /old_locus_tag="FDP43_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262957.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(<3239..3730)
                     /locus_tag="FDP43_RS25785"
                     /old_locus_tag="FDP43_25795"
                     /pseudo=""
     CDS             complement(<3239..3730)
                     /locus_tag="FDP43_RS25785"
                     /old_locus_tag="FDP43_25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /name="transposase zinc-binding domain-containing protein"
     gene            complement(4284..4568)
                     /locus_tag="FDP43_RS25790"
                     /old_locus_tag="FDP43_25800"
     CDS             complement(4284..4568)
                     /locus_tag="FDP43_RS25790"
                     /old_locus_tag="FDP43_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            4767..>5408
                     /locus_tag="FDP43_RS26675"
                     /pseudo=""
     CDS             4767..>5408
                     /locus_tag="FDP43_RS26675"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            5515..5928
                     /locus_tag="FDP43_RS26680"
     CDS             5515..5928
                     /locus_tag="FDP43_RS26680"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            6027..6896
                     /locus_tag="FDP43_RS26685"
     CDS             6027..6896
                     /locus_tag="FDP43_RS26685"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_229382459.1"
                     /translation="MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMD
                     RGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            6957..7169
                     /locus_tag="FDP43_RS25800"
                     /old_locus_tag="FDP43_25810"
     CDS             6957..7169
                     /locus_tag="FDP43_RS25800"
                     /old_locus_tag="FDP43_25810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            7171..7377
                     /gene="urfF"
                     /locus_tag="FDP43_RS25805"
                     /old_locus_tag="FDP43_25815"
     CDS             7171..7377
                     /gene="urfF"
                     /locus_tag="FDP43_RS25805"
                     /old_locus_tag="FDP43_25815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     Typing          4523..4678
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          19..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          4768..6893
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 cccccccact ctattgtaaa caagacattt ttatctttta tattcaatgg cttattttcc
     1741 tgctaattgg taataccatg aaaaatacca tgctcagaaa aggcttaaca atattttgaa
     1801 aaattgccta ctgagcgctg ccgcacagct ccataggccg ctttcctggc tttgcttcca
     1861 gatgtatgct attctgctcc tgcagctaat ggatcaccgc aaacaggtta ctcgcctggg
     1921 gattcccttt cgacccgagc atccgtttta aatttccgaa cttgtaggaa ttatacataa
     1981 ataaaaacaa ttcttactta acaaaatcag tctataattg taatatatgg atatgaataa
     2041 aaacactaaa ataaaaaaca aaaatttcaa cattaaagac tcacagaatt ttttgcataa
     2101 tactaaatta gtcgaagatt tgctttttaa aagcaatata actaaggagg attttgttgt
     2161 tgagattggg cctggaaaag gcataataac caaggcatta agcaaaatct gcaaagctgt
     2221 taatgctatt gagttcgata gtgtattggc tgataagttg agccatgaat ttaaaagttc
     2281 aaatgtgtct attattgaag ccgatttttt aaaatacaat ttaccagacc ataattataa
     2341 agttttttca aacattccat ttaacataac ggcaagtatt ttaaataaat tgttagatag
     2401 tgagaaccca cccttagata cttttttaat tatgcaatat gaaccttttt taaagtatgc
     2461 gggtgcacca tcttacaagg agtcttataa atctttatta tataaaccat ttttcaaaac
     2521 taacatattg catagcttta gcaaatttga ttttaagcca gctccaaacg caaacattat
     2581 tttgggccaa ttttcttata aagactttac agatataaac cttgaagaca ggcatgcttg
     2641 gaaagatttt ttagcctttg tctttttaga aaagggagtt acatttaaag aaaaaacaaa
     2701 acgaattttt agttataagc aacaaaaaat aattttaaaa gaaagccgaa ttaatgatga
     2761 ttcaaatata agtaattgga gttatgaatt ttggctaaaa atgtttaaac tctataattc
     2821 gaacatggta agcaaggata aaaaagtttt agttaacaat tcgtataaaa gaatgttaga
     2881 acatgagtct agtttagaaa agattcatag aaatagaaag caaaataaca gaaaatagat
     2941 aaacggcaga taaggaaaag ctaaatgtta aaattaaagc tagaaaacat aaacaaaact
     3001 ttcgatggga aaaactttgt tgttaaaaat tttaatttgg attagggtct gttgaacatt
     3061 catagcttca accaaacata tgcacaggcc aaagcaacat tgctactgta gctgcttttt
     3121 agcttatcat agcgagttgc taagcctcta aagtgcttta atgtcgcaaa catattctcc
     3181 acgacatgcc gcgctttata aatatgccag tccatatggc tattgtcttt taaactaggg
     3241 tatggcccgc tttcttgacc aggtgcgtgg caatgacgcg gtaaacgatg cccagcaccc
     3301 accccatgat ctcgggccgg ctggcaaaca ggaaacgcag ctgaaacggg aagctcaaca
     3361 cccactgacg catgggttgt tcaggcagta cttcatcaac cagcaaggcg gcactttcgg
     3421 ccatccgccg cgccccacag ctcgggcaga aaccgcgacg cttacagctg aaagcgacca
     3481 ggtgctcggc gtggcaagac tcgcagcgaa cccgtagaaa gccatgctcc agccgcccgc
     3541 attggagaaa ttcttcaaat tcccgttgca catagcccgg caattccttt ccctgctctg
     3601 ccataagcgc agcgaatgcc gggtaatact cgtcaacgat ctgatagaga agggtttgct
     3661 cgggtcggtg gctctggtaa cgaccagtat cccgatcccg gctggccgtc ctggccgcca
     3721 catgaggcat gttccgcgtc cttgcaatac tgtgtttaca tacagtctat cgcttagcgg
     3781 aaagttcttt taccctcagc cgaaatgcct gccgttgcta gacattgcca gccagtgccc
     3841 gtcactcccg tactaactgt cacgaacccc tgcaataact gtcacgcccc cctgcaataa
     3901 ctgtcacgaa cccctgcaat aactgtcacg cccccaaacc tgcaaaccca gcaggggcgg
     3961 gggctggcgg ggtgttggaa aaatccatcc atgattatct aagaataatc cactaggcgc
     4021 ggttatcagc gcccttgtgg ggcgctgctg cccttgccca atatgcccgg ccagaggccg
     4081 gatagctggt ctattcgctg cgctaggcta cacaccgccc caccgctgcg cggcaggggg
     4141 aaaggcgggc aaagcccgct aaaccccaca ccaaaccccg cagaaatacg ctggagcgct
     4201 tttagccgct ttagcggcct ttccccctac ccgaagggtg ggggcgcgtg tgcagccccg
     4261 cagggcctgt ctcggtcgat cattcagccc ggctcatcct tctggcgtgg cggcagaccg
     4321 aacaaggcgc ggtcgtggtc gcgttcaagg tacgcatcca ttgccgccat gagccgatcc
     4381 tccggccact cgctgctgtt caccttggcc aaaatcatgg cccccaccag caccttgcgc
     4441 cttgtttcgt tcttgcgctc ttgctgctgt tcccttgccc gcacccgctg aatttcggca
     4501 ttgattcgcg ctcgttgttc ttcgagcttg gccagccgat ccgccgcctt gttgctcccc
     4561 ttaaccatct tgacacccca ttgttaatgt gctgtctcgt aggctatcat ggaggcacag
     4621 cggcggcaat cccgacccta ctttgtaggg gagggcgcac ttaccggttt ctcttcgaga
     4681 aactggccta acggccaccc ttcgggcggt gcgctctccg agggccattg catggagccg
     4741 aaaagcaaaa gcaacagcga ggcagcatgg cgatttatca ccttacggcg aaaaccggca
     4801 gcaggtcggg cggccaatcg gccagggcca aggccgacta catccagcgc gaaggcaagt
     4861 atgcccgcga catggatgaa gtcttgcacg ccgaatccgg gcacatgccg gagttcgtcg
     4921 agcggcccgc cgactactgg gatgctgccg acctgtatga acgcgccaat gggcggctgt
     4981 tcaaggaggt cgaatttgcc ctgccggtcg agctgaccct cgaccagcag aaggcgctgg
     5041 cgtccgagtt cgcccagcac ctgaccggtg ccgagcgcct gccgtatacg ctggccatcc
     5101 atgccggtgg cggcgagaac ccgcactgcc acctgatgat ctccgagcgg atcaatgacg
     5161 gcatcgagcg gcccgccgct cagtggttca agcggtacaa cggcaagacc ccggagaagg
     5221 gcggggcaca gaagaccgaa gcgctcaagc ccaaggcatg gcttgagcag acccgcgagg
     5281 catgggccga ccatgccaac cgggcattag agcgggctgg ccacgacgcc cgcattgacc
     5341 acagaacact tgaggcgcag ggcatcgagc gcctgcccgg tgttcacctg gggccgaacg
     5401 tggtggagat ggaaggccgg ggcatccgca ccgaccgggc agacgtggcc ctgaacatcg
     5461 acaccgccaa cgcccagatc atcgacttac aggaataccg ggaggcaata gaccatgaac
     5521 gcaatcgaca gagtgaagaa atccagaggc atcaacgagt tagcggagca gatcgaaccg
     5581 ctggcccaga gcatggcgac actggccgac gaagcccggc aggtcatgag ccagacccag
     5641 caggccagcg aggcgcaggc ggcggagtgg ctgaaagccc agcgccagac aggggcggca
     5701 tgggtggagc tggccaaaga gttgcgggag gtagccgccg aggtgagcag cgccgcgcag
     5761 agcgcccgga gcgcgtcgcg ggggtggcac tggaagctat ggctaaccgt gatgctggct
     5821 tccatgatgc ctacggtggt gctgctgatc gcatcgttgc tcttgctcga cctgacgcca
     5881 ctgacaaccg aggacggctc gatctggctg cgcttggtgg cccgatgaag aacgacagga
     5941 ctttgcaggc cataggccga cagctcaagg ccatgggctg tgagcgcttc gatatcggcg
     6001 tcagggacgc caccaccggc cagatgatga accgggaatg gtcagccgcc gaagtgctcc
     6061 agaacacgcc atggctcaag cggatgaatg cccagggcaa tgacgtgtat atcaggcccg
     6121 ccgagcagga gcggcatggt ctggtgctgg tggacgacct cagcgagttt gacctggatg
     6181 acatgaaagc cgagggccgg gagcctgccc tggtagtgga aaccagcccg aagaactatc
     6241 aggcatgggt caaggtggcc gacgccgcag gcggtgaact tcgggggcag attgcccgga
     6301 cgctggccag cgagtacgac gccgacccgg ccagcgccga cagccgccac tatggccgct
     6361 tggcgggctt caccaaccgc aaggacaagc acaccacccg cgccggttat cagccgtggg
     6421 tgctgctgcg tgaatccaag ggcaagaccg ccaccgctgg cccggcgctg gtgcagcagg
     6481 ctggccagca gatcgagcag gcccagcggc agcaggagaa ggcccgcagg ctggccagcc
     6541 tcgaactgcc cgagcggcag cttagccgcc accggcgcac ggcgctggac gagtaccgca
     6601 gcgagatggc cgggctggtc aagcgcttcg gtgatgacct cagtaagtgc gactttatcg
     6661 ccgcgcagaa gctggccagc cggggccgca gtgccgagga aatcggcaag gccatggccg
     6721 aggccagccc agcgctggca gagcgcaagc ccggccacga agcggattac atcgagcgca
     6781 ccgtcagcaa ggtcatgggt ctgcccagcg tccagcttgc gcgggccgag ctggcacggg
     6841 caccggcacc ccgccagcga ggcatggaca ggggcgggcc agatttcagc atgtagtgct
     6901 tgcgttggta ctcacgcctg ttatactatg agtactcacg cacagaaggg ggttttatgg
     6961 aatacgaaaa aagcgcttca gggtcggtct acctgatcaa aagtgacaag ggctattggt
     7021 tgcccggtgg ctttggttat acgtcaaaca aggccgaggc tggccgcttt tcagtcgctg
     7081 atatggccag ccttaacctt gacggctgca ccttgtcctt gttccgcgaa gacaagcctt
     7141 tcggccccgg caagtttctc ggtgactgat atgaaagacc aaaaagacaa gcagaccggc
     7201 gacctgctgg ccagccctga cgctgtacgc caagcgcgat atgccgagcg catgaaggcc
     7261 aaagggatgc gtcagcgcaa gttctggctg accgacgacg aatacgaggc gctgcgcgag
     7321 tgcctggaag aactcagagc ggcgcagggc gggggtagtg accccgccag cgcctaacca
     7381 ccaactgcct gcaaaggagg caatca
//
