LOCUS       NZ_CP040032             4938 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KPC160121 plasmid pCol4401-L121,
            complete sequence.
ACCESSION   NZ_CP040032
VERSION     NZ_CP040032.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11246289
            Assembly: GCF_011106605.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Lai,Y., Lu,M., Tang,H., Chiou,C. and Chen,Y.
  TITLE     OXA-48 Klebsiella pneumoniae in central Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Lai,Y., Lu,M., Tang,H., Chiou,C. and Chen,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2019) Microbiology and Immunology, Chung Shan
            Medical University, No.110, Sec.1, Jianguo N.Rd, Taichung 414,
            Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. May-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 07:33:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,798
            CDSs (total)                      :: 5,674
            Genes (coding)                    :: 5,562
            CDSs (with protein)               :: 5,562
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 49 of 112
            Pseudo Genes (incomplete)         :: 61 of 112
            Pseudo Genes (internal stop)      :: 12 of 112
            Pseudo Genes (multiple problems)  :: 10 of 112
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040032.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPC160121"
                     /isolation_source="pus"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCol4401-L121"
                     /geo_loc_name="Taiwan:Taichung"
                     /lat_lon="23.3 N 120.9 E"
                     /collection_date="2015-01-30"
                     /collected_by="CSMUH"
     gene            join(4773..4938,1..440)
                     /locus_tag="FDK25_RS29790"
     CDS             join(4773..4938,1..440)
                     /locus_tag="FDK25_RS29790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047715645.1"
                     /translation="MQDRSVVNRQGFNPEIDLPYSLRISDFEHAMEDIYDFFHDVNNLL
                     SNKGLHRLDDMMRPAAMSGLISDMITASLAKFSRSLVENKHFNGHPDLVVRGRYPNDSI
                     SSGEEGIEIKTTRKNGGAVDTHGARSQWMCVFVYNVDNETEPASDRKSMKFTEVYLCHV
                     DENDFRRNDRGALGTRTATLHKEGLKKLRESWVYKDLV"
                     /name="hypothetical protein"
     gene            complement(437..1555)
                     /locus_tag="FDK25_RS29550"
                     /old_locus_tag="FDK25_29315"
     CDS             complement(437..1555)
                     /locus_tag="FDK25_RS29550"
                     /old_locus_tag="FDK25_29315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402750.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="WP_117064210.1"
                     /translation="MSLAPGVVRDSIIECFLRLGREASIEEITKAVYLKIGEISKSSVR
                     SYLSLNTPGLFERVDRGVYRLNGDAGITPSLDLTSSPKTVGKSTIFFDDAIEWLKRQKD
                     NSIHAVVTDPPYGLVEYTKQQTEKLRAGKGGIWRKPPSFDGHKRAPLPRFTVLREEDLR
                     NISLFFKELSSQVERVVVPGGNIVIASNPLLSHIVADSMSTSGLELRGYIVRQVMTMRG
                     GDRPKNAHDEFGDVSVMPRSQWEPWIVLRKPLDGRVQDNLRKWKTGGFRRISADRPFGD
                     VIKSHPTPAHEKKIAPHPSLKPQNFMRQLVRAVLPLGEGIILDPFMGGGSTLAAANHIG
                     YESIGVEIDREFFDLAEKAIPALSALPSKENN"
                     /name="DNA methyltransferase"
     gene            complement(2031..2246)
                     /locus_tag="FDK25_RS29555"
                     /old_locus_tag="FDK25_29320"
     CDS             complement(2031..2246)
                     /locus_tag="FDK25_RS29555"
                     /old_locus_tag="FDK25_29320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_064147933.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2253..2750)
                     /locus_tag="FDK25_RS29560"
                     /old_locus_tag="FDK25_29325"
     CDS             complement(2253..2750)
                     /locus_tag="FDK25_RS29560"
                     /old_locus_tag="FDK25_29325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2791..3489)
                     /locus_tag="FDK25_RS30215"
                     /old_locus_tag="FDK25_29330"
                     /pseudo=""
     CDS             complement(<2791..3489)
                     /locus_tag="FDK25_RS30215"
                     /old_locus_tag="FDK25_29330"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3428..3769)
                     /locus_tag="FDK25_RS29570"
                     /old_locus_tag="FDK25_29335"
     CDS             complement(3428..3769)
                     /locus_tag="FDK25_RS29570"
                     /old_locus_tag="FDK25_29335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_074181101.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     QEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4393..4506
                     /locus_tag="FDK25_RS30220"
                     /old_locus_tag="FDK25_29350"
     CDS             4393..4506
                     /locus_tag="FDK25_RS30220"
                     /old_locus_tag="FDK25_29350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     Typing          complement(3857..3936)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.296"
                     /coverage="93.0"
                     /evalue="6.62e-29"
     Typing          3987..4506
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.587"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1916..3438)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.803"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3435..3748)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="8.420000000000001e-58"
ORIGIN
        1 tgatgagacc agcagctatg tctggcctca tatcagatat gatcacagct tccctcgcta
       61 aattttcaag atcgcttgtt gaaaacaagc atttcaatgg acatccagac ctagttgtta
      121 ggggcaggta tccgaatgac agtatctcct caggagaaga aggcattgaa ataaaaacca
      181 cccggaaaaa tgggggggca gtcgatactc acggggccag atctcaatgg atgtgcgtat
      241 ttgtctacaa tgtcgataat gagaccgaac cggcctcaga cagaaagtcg atgaaattta
      301 ccgaggtata tctttgccac gttgatgaaa atgacttccg tcgtaatgat cgtggtgcat
      361 taggaaccag aacagcaacc cttcacaaag aagggttgaa gaagctcaga gaaagctggg
      421 tttacaagga cttagtttag ttattttcct tgctaggaag agctgatagc gcggggatag
      481 ccttctccgc caaatcaaag aactctcggt ctatttcaac tccaatgctc tcataaccta
      541 tatgatttgc agctgccaat gtggagcctc cccccataaa tggatcgaga ataatcccct
      601 ctcccaaagg aagcactgcc ctcactaatt gtcgcatgaa attttgcggc tttaatgagg
      661 gatgtggtgc tatctttttt tcgtgtgctg gcgtcggatg agatttgatg acgtcgccaa
      721 atggcctatc agctgaaatc ctcctgaatc cacctgtttt ccactttctt agattatcct
      781 gaacccttcc atctagaggc tttctcaaca ctatccatgg ttcccattga gaacgaggca
      841 tgacagaaac atctccaaat tcatcatggg cgtttttggg tctatcccca cctctcatgg
      901 tcataacctg acgtacaatg taacctcgta gctctaagcc agacgtagac atagagtccg
      961 cgactatatg ggatagtaga gggttggatg cgataactat attcccgcca ggaacaacca
     1021 ccctttcgac ttgcgaggat agttctttga aaaataatga tatatttctg aggtcttctt
     1081 cccgcaaaac cgtaaagcga ggtaatggcg ctcgcttatg tccatcaaag gaaggtggct
     1141 ttctccaaat accacctttc cctgccctta gtttctcagt ctgttgtttt gtatactcga
     1201 ctaatccgta aggaggatca gttacaactg catgaattga attatctttt tgtcttttta
     1261 accattcaat tgcatcgtca aaaaaaatcg tcgatttccc aaccgtctta ggtgaagacg
     1321 tcaaatctag ggagggagta ataccagcat caccatttaa gcgatagaca cctcggtcta
     1381 ctcgctcgaa cagacctggg gtatttaaac tcagatatga gcgaacagaa gactttgaaa
     1441 tttctccaat ttttaaataa acagcttttg ttatctcctc aatagaagct tctcttccaa
     1501 gccttaaaaa acattcaatt atcgagtcac gaacaacacc aggagccaaa gacatgttta
     1561 taaatccagt atttttatat aaaataataa tactaactag acgtcaggac gtcaatttag
     1621 tttaaaagag acgtcaaaaa agtatacatt aacttttttt ttaccataac atcccttacc
     1681 cgcaccgcgc cggaggccct cagggcgttt ctgatggctg acgggcaaaa ggtggtggca
     1741 agtacaccga aaaccaccag aaacgcacct gatttatttt acgcatatgc atcacttatt
     1801 cacatcactg catagccggt gatttcggtt aaaaaagact ctctgtgtgc tgaaatcgca
     1861 gaaaatggcc atgatccccg tcacgctcag gccacctgag acgggcgttt atcagtctcc
     1921 atactccctg tcaccatgag ataatgtgcg ttgccgttcc ggtaccagat ctgctgtctt
     1981 ctctggttcc ttttccgttc tttgcccgta tcgctgttgc tcaatctcat tcagcctgtc
     2041 gataaccggc gcagctgctc gctcaaactc tgcacctgcc tgctcaagcc cgacacgcgc
     2101 tcgctcagcg ccgcgttctc ccgttgcata agaccagaca tcttccgcca ttccccgaag
     2161 gcgctctccc actcgtccag ccttctcgag tagtcctgct gtagctgctc taacgcgctc
     2221 agcaattgtt tttccagttc cgtcattctg ctctactccc gtaccagctt caccgcgttc
     2281 cgcgtcttgt tgtccaccgt ccggcccgtt tcggctttca tcccctccgg cagcaccaga
     2341 aaccgtccgt tgcggttctc cagataggtc acgccccacg tctgcatgtg cagtttcgac
     2401 agcgtatctc tctgctgcct gatttccgcc aggttcgccg ctatccggtt cccctggtac
     2461 cacagcaccc cgctcaggct ggcgaacagc agccctgaca ccatggtgat ggtcagccac
     2521 gtccgcccga ccattcgcag cacgctcagc cggttcgact gcatcgccgc gttcatcccc
     2581 tgttcgtggg cgctgatggc gtcgttgatt ctcttcgcgc ttgcgttcag ttccgatttg
     2641 acaacgttct cgtgcttgct gaatgcggct ttcagcctct cttcggtaag ctgctgctgt
     2701 gctttcgatt tctcctcgaa gtccctcgag gccgttaaaa gactgctcat aaatggctcc
     2761 cctcagtcgg atatttcgcc ccccgtccgg gtcggcaatg ctgatactgt tttttgtggt
     2821 tctgaccacc tcaaaaccgg ccgctgtgag cgcccctgtg acgtcatcac gcgttttcag
     2881 ctctcctgat gaggctaaca tcagcaaacc ccgtgtaatc gtctccgcgg cttcctgctt
     2941 cgtttctggc agtcctgacg gcgtgaccag cgcccgccgg ttctccgggt cgttcggatc
     3001 atgcagcccc agcctgccgt tgaccactgt ctgccacgca tcgatgcgcg gccggtcggc
     3061 acgatcgtaa tacggctgga ggcgtttccc cgtcagtaat tcggtattgg ggatcagaaa
     3121 attcagctca agacgaccct tgtctgcgtg ttccacccac agaatgctgt actgatcttt
     3181 atcgagtccg ggcatcagaa cccgctcaaa gctggccatc agtttttcac gctgacccgg
     3241 cggcaggtcc gcctccgcga atgacagcac cccggaggta tattttttca catacggcga
     3301 cgcatcaatc agctcccgga cttcttccgg cttcccctgc aggacgctgg caccttcacg
     3361 ctgccggtct ttacccagaa gatagtccac cggaccggcg cccccgccgc gaccccgggg
     3421 atggaattta acaatcatcg tccggccctt tttcctgtac ggcatgccgc aaccgttcca
     3481 gaccggcatc gatcgccatc agcgctgcca cgatctgcac ccggtcatga ccggcaccac
     3541 cggcgttcac tttccgggcg atctggttca ggttattgcc catgccggca agctgacgca
     3601 gcagcgccgg cgaaatcgag gggagcctgc cggcgcgtgt cggtttctca tcaaggcagg
     3661 tctggcgcat ccacgccgcc agttgttttc cgtcgcaccg ctcaagcaac cgccggtgtt
     3721 cgtcttccgt cacccacatc gtgagcatct tgttgcgttt atctgccaga atcgcctcct
     3781 gataaccccg tgacggacgg gaaagccttt cccggtcggc gcggggttgg acaagccgca
     3841 ggcgcgtcag tggtttttag cgggtttcgg ggcgcagccc tgaaccagtc acgtagcgct
     3901 agcggagtgt atactggctt actatagtgg catcggtatg ggtatcagcg aaagtgctat
     3961 atatggcagg gatgacatgc agcgacggtg cgtaaggcag ataatggtag tgccgggggt
     4021 atgacgcctc ctcgctcact cggtcgctgc gctcctgccg tgagactgcg gcgggcgtta
     4081 ccagctcacg agtgacgaga gaaagaaata gcgctcacgt caggaaggcc agaaaaaagg
     4141 atgcggcgtt gccgtttttc cataggctcc gcccccctga cgagcatcac agaatctgat
     4201 gctcaaatca gtggtggcga aacccgacag gactataaag atcccaggcg tttccccctg
     4261 gtagctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     4321 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     4381 aagctggact gtatgcacga accccccgtt cagtccgact gctgcgcctt atccggtaac
     4441 tatcgtcttg agtccaaccc ggaaagacat gcaaaagcgc cactggcagc agccactggt
     4501 aactgaaaag gtggatttag atattgagag ttcttgaagt ggtgacctga ctgcggctac
     4561 actagaagga cagtatttgg tatctgcgct ccaccaagcc agttacctgg ttaagcagtt
     4621 ccccaactga cttaaccttc gattaaaccg cctccccagg cggttttttc gtttcaagag
     4681 gctacgccca aacgacaatg atcgtcaagg atcttcttgg taagatatga caaagagctt
     4741 tcgaactttt atatcttatt caaggaacta agatgcaaga caggtcagta gttaatcgac
     4801 agggatttaa cccagagatc gatctgccgt attcgctaag gattagtgat tttgagcacg
     4861 cgatggaaga tatttatgac ttcttccatg atgtgaacaa cttactatca aacaaaggtt
     4921 tgcataggct tgatgaca
//
