LOCUS       NZ_CP040027             4938 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KPC160132 plasmid pCol4401-L132,
            complete sequence.
ACCESSION   NZ_CP040027
VERSION     NZ_CP040027.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11246291
            Assembly: GCF_011129215.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Lai,Y., Lu,M., Tang,H., Chiou,C. and Chen,Y.
  TITLE     OXA-48 Klebsiella pneumoniae in central Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Lai,Y., Lu,M., Tang,H., Chiou,C. and Chen,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2019) Microbiology and Immunology, Chung Shan
            Medical University, No.110, Sec.1, Jianguo N.Rd, Taichung 414,
            Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. May-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 08:20:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,842
            CDSs (total)                      :: 5,717
            Genes (coding)                    :: 5,611
            CDSs (with protein)               :: 5,611
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 47 of 106
            Pseudo Genes (incomplete)         :: 57 of 106
            Pseudo Genes (internal stop)      :: 12 of 106
            Pseudo Genes (multiple problems)  :: 10 of 106
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040027.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPC160132"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCol4401-L132"
                     /geo_loc_name="Taiwan:Taichung"
                     /lat_lon="23.3 N 120.9 E"
                     /collection_date="2015-04-01"
                     /collected_by="CSMUH"
     gene            263..760
                     /locus_tag="FDK24_RS29805"
                     /old_locus_tag="FDK24_29585"
     CDS             263..760
                     /locus_tag="FDK24_RS29805"
                     /old_locus_tag="FDK24_29585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            767..982
                     /locus_tag="FDK24_RS29810"
                     /old_locus_tag="FDK24_29590"
     CDS             767..982
                     /locus_tag="FDK24_RS29810"
                     /old_locus_tag="FDK24_29590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_064147933.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1458..2576
                     /locus_tag="FDK24_RS29815"
                     /old_locus_tag="FDK24_29595"
     CDS             1458..2576
                     /locus_tag="FDK24_RS29815"
                     /old_locus_tag="FDK24_29595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402750.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="WP_117064210.1"
                     /translation="MSLAPGVVRDSIIECFLRLGREASIEEITKAVYLKIGEISKSSVR
                     SYLSLNTPGLFERVDRGVYRLNGDAGITPSLDLTSSPKTVGKSTIFFDDAIEWLKRQKD
                     NSIHAVVTDPPYGLVEYTKQQTEKLRAGKGGIWRKPPSFDGHKRAPLPRFTVLREEDLR
                     NISLFFKELSSQVERVVVPGGNIVIASNPLLSHIVADSMSTSGLELRGYIVRQVMTMRG
                     GDRPKNAHDEFGDVSVMPRSQWEPWIVLRKPLDGRVQDNLRKWKTGGFRRISADRPFGD
                     VIKSHPTPAHEKKIAPHPSLKPQNFMRQLVRAVLPLGEGIILDPFMGGGSTLAAANHIG
                     YESIGVEIDREFFDLAEKAIPALSALPSKENN"
                     /name="DNA methyltransferase"
     gene            complement(2573..3178)
                     /locus_tag="FDK24_RS29820"
                     /old_locus_tag="FDK24_29600"
     CDS             complement(2573..3178)
                     /locus_tag="FDK24_RS29820"
                     /old_locus_tag="FDK24_29600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047715645.1"
                     /translation="MQDRSVVNRQGFNPEIDLPYSLRISDFEHAMEDIYDFFHDVNNLL
                     SNKGLHRLDDMMRPAAMSGLISDMITASLAKFSRSLVENKHFNGHPDLVVRGRYPNDSI
                     SSGEEGIEIKTTRKNGGAVDTHGARSQWMCVFVYNVDNETEPASDRKSMKFTEVYLCHV
                     DENDFRRNDRGALGTRTATLHKEGLKKLRESWVYKDLV"
                     /name="hypothetical protein"
     gene            complement(3445..3558)
                     /locus_tag="FDK24_RS30470"
                     /old_locus_tag="FDK24_29605"
     CDS             complement(3445..3558)
                     /locus_tag="FDK24_RS30470"
                     /old_locus_tag="FDK24_29605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            4182..4523
                     /locus_tag="FDK24_RS29830"
                     /old_locus_tag="FDK24_29620"
     CDS             4182..4523
                     /locus_tag="FDK24_RS29830"
                     /old_locus_tag="FDK24_29620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_074181101.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     QEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          3913..4088
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="80.663"
                     /coverage="100.0"
                     /evalue="1.67e-28"
     Typing          complement(3446..3965)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.587"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2..342
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.333"
                     /coverage="93.0"
                     /evalue="3.22e-52"
     Typing          4204..4517
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="8.420000000000001e-58"
     Typing          4514..4938
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.22e-86"
ORIGIN
        1 ctggggctgc atgatccgaa cgacccggag aaccggcggg cgctggtcac gccgtcagga
       61 ctgccagaaa cgaagcagga agccgcggag acgattacac ggggtttgct gatgttagcc
      121 tcatcaggag agctgaaaac gcgtgatgac gtcacagggg cgctcacagc ggccggtttt
      181 gaggtggtca gaaccacaaa aaacagtatc agcattgccg acccggacgg ggggcgaaat
      241 atccgactga ggggagccat ttatgagcag tcttttaacg gcctcgaggg acttcgagga
      301 gaaatcgaaa gcacagcagc agcttaccga agagaggctg aaagccgcat tcagcaagca
      361 cgagaacgtt gtcaaatcgg aactgaacgc aagcgcgaag agaatcaacg acgccatcag
      421 cgcccacgaa caggggatga acgcggcgat gcagtcgaac cggctgagcg tgctgcgaat
      481 ggtcgggcgg acgtggctga ccatcaccat ggtgtcaggg ctgctgttcg ccagcctgag
      541 cggggtgctg tggtaccagg ggaaccggat agcggcgaac ctggcggaaa tcaggcagca
      601 gagagatacg ctgtcgaaac tgcacatgca gacgtggggc gtgacctatc tggagaaccg
      661 caacggacgg tttctggtgc tgccggaggg gatgaaagcc gaaacgggcc ggacggtgga
      721 caacaagacg cggaacgcgg tgaagctggt acgggagtag agcagaatga cggaactgga
      781 aaaacaattg ctgagcgcgt tagagcagct acagcaggac tactcgagaa ggctggacga
      841 gtgggagagc gccttcgggg aatggcggaa gatgtctggt cttatgcaac gggagaacgc
      901 ggcgctgagc gagcgcgtgt cgggcttgag caggcaggtg cagagtttga gcgagcagct
      961 gcgccggtta tcgacaggct gaatgagatt gagcaacagc gatacgggca aagaacggaa
     1021 aaggaaccag agaagacagc agatctggta ccggaacggc aacgcacatt atctcatggt
     1081 gacagggagt atggagactg ataaacgccc gtctcaggtg gcctgagcgt gacggggatc
     1141 atggccattt tctgcgattt cagcacacag agagtctttt ttaaccgaaa tcaccggcta
     1201 tgcagtgatg tgaataagtg atgcatatgc gtaaaataaa tcaggtgcgt ttctggtggt
     1261 tttcggtgta cttgccacca ccttttgccc gtcagccatc agaaacgccc tgagggcctc
     1321 cggcgcggtg cgggtaaggg atgttatggt aaaaaaaaag ttaatgtata cttttttgac
     1381 gtctctttta aactaaattg acgtcctgac gtctagttag tattattatt ttatataaaa
     1441 atactggatt tataaacatg tctttggctc ctggtgttgt tcgtgactcg ataattgaat
     1501 gttttttaag gcttggaaga gaagcttcta ttgaggagat aacaaaagct gtttatttaa
     1561 aaattggaga aatttcaaag tcttctgttc gctcatatct gagtttaaat accccaggtc
     1621 tgttcgagcg agtagaccga ggtgtctatc gcttaaatgg tgatgctggt attactccct
     1681 ccctagattt gacgtcttca cctaagacgg ttgggaaatc gacgattttt tttgacgatg
     1741 caattgaatg gttaaaaaga caaaaagata attcaattca tgcagttgta actgatcctc
     1801 cttacggatt agtcgagtat acaaaacaac agactgagaa actaagggca gggaaaggtg
     1861 gtatttggag aaagccacct tcctttgatg gacataagcg agcgccatta cctcgcttta
     1921 cggttttgcg ggaagaagac ctcagaaata tatcattatt tttcaaagaa ctatcctcgc
     1981 aagtcgaaag ggtggttgtt cctggcggga atatagttat cgcatccaac cctctactat
     2041 cccatatagt cgcggactct atgtctacgt ctggcttaga gctacgaggt tacattgtac
     2101 gtcaggttat gaccatgaga ggtggggata gacccaaaaa cgcccatgat gaatttggag
     2161 atgtttctgt catgcctcgt tctcaatggg aaccatggat agtgttgaga aagcctctag
     2221 atggaagggt tcaggataat ctaagaaagt ggaaaacagg tggattcagg aggatttcag
     2281 ctgataggcc atttggcgac gtcatcaaat ctcatccgac gccagcacac gaaaaaaaga
     2341 tagcaccaca tccctcatta aagccgcaaa atttcatgcg acaattagtg agggcagtgc
     2401 ttcctttggg agaggggatt attctcgatc catttatggg gggaggctcc acattggcag
     2461 ctgcaaatca tataggttat gagagcattg gagttgaaat agaccgagag ttctttgatt
     2521 tggcggagaa ggctatcccc gcgctatcag ctcttcctag caaggaaaat aactaaacta
     2581 agtccttgta aacccagctt tctctgagct tcttcaaccc ttctttgtga agggttgctg
     2641 ttctggttcc taatgcacca cgatcattac gacggaagtc attttcatca acgtggcaaa
     2701 gatatacctc ggtaaatttc atcgactttc tgtctgaggc cggttcggtc tcattatcga
     2761 cattgtagac aaatacgcac atccattgag atctggcccc gtgagtatcg actgcccccc
     2821 catttttccg ggtggttttt atttcaatgc cttcttctcc tgaggagata ctgtcattcg
     2881 gatacctgcc cctaacaact aggtctggat gtccattgaa atgcttgttt tcaacaagcg
     2941 atcttgaaaa tttagcgagg gaagctgtga tcatatctga tatgaggcca gacatagctg
     3001 ctggtctcat catgtcatca agcctatgca aacctttgtt tgatagtaag ttgttcacat
     3061 catggaagaa gtcataaata tcttccatcg cgtgctcaaa atcactaatc cttagcgaat
     3121 acggcagatc gatctctggg ttaaatccct gtcgattaac tactgacctg tcttgcatct
     3181 tagttccttg aataagatat aaaagttcga aagctctttg tcatatctta ccaagaagat
     3241 ccttgacgat cattgtcgtt tgggcgtagc ctcttgaaac gaaaaaaccg cctggggagg
     3301 cggtttaatc gaaggttaag tcagttgggg aactgcttaa ccaggtaact ggcttggtgg
     3361 agcgcagata ccaaatactg tccttctagt gtagccgcag tcaggtcacc acttcaagaa
     3421 ctctcaatat ctaaatccac cttttcagtt accagtggct gctgccagtg gcgcttttgc
     3481 atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc agtcggactg
     3541 aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt
     3601 caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg
     3661 caggaacagg agagcgcacg agggagctac cagggggaaa cgcctgggat ctttatagtc
     3721 ctgtcgggtt tcgccaccac tgatttgagc atcagattct gtgatgctcg tcaggggggc
     3781 ggagcctatg gaaaaacggc aacgccgcat ccttttttct ggccttcctg acgtgagcgc
     3841 tatttctttc tctcgtcact cgtgagctgg taacgcccgc cgcagtctca cggcaggagc
     3901 gcagcgaccg agtgagcgag gaggcgtcat acccccggca ctaccattat ctgccttacg
     3961 caccgtcgct gcatgtcatc cctgccatat atagcacttt cgctgatacc cataccgatg
     4021 ccactatagt aagccagtat acactccgct agcgctacgt gactggttca gggctgcgcc
     4081 ccgaaacccg ctaaaaacca ctgacgcgcc tgcggcttgt ccaaccccgc gccgaccggg
     4141 aaaggctttc ccgtccgtca cggggttatc aggaggcgat tctggcagat aaacgcaaca
     4201 agatgctcac gatgtgggtg acggaagacg aacaccggcg gttgcttgag cggtgcgacg
     4261 gaaaacaact ggcggcgtgg atgcgccaga cctgccttga tgagaaaccg acacgcgccg
     4321 gcaggctccc ctcgatttcg ccggcgctgc tgcgtcagct tgccggcatg ggcaataacc
     4381 tgaaccagat cgcccggaaa gtgaacgccg gtggtgccgg tcatgaccgg gtgcagatcg
     4441 tggcagcgct gatggcgatc gatgccggtc tggaacggtt gcggcatgcc gtacaggaaa
     4501 aagggccgga cgatgattgt taaattccat ccccggggtc gcggcggggg cgccggtccg
     4561 gtggactatc ttctgggtaa agaccggcag cgtgaaggtg ccagcgtcct gcaggggaag
     4621 ccggaagaag tccgggagct gattgatgcg tcgccgtatg tgaaaaaata tacctccggg
     4681 gtgctgtcat tcgcggaggc ggacctgccg ccgggtcagc gtgaaaaact gatggccagc
     4741 tttgagcggg ttctgatgcc cggactcgat aaagatcagt acagcattct gtgggtggaa
     4801 cacgcagaca agggtcgtct tgagctgaat tttctgatcc ccaataccga attactgacg
     4861 gggaaacgcc tccagccgta ttacgatcgt gccgaccggc cgcgcatcga tgcgtggcag
     4921 acagtggtca acggcagg
//
