LOCUS       NZ_CP039995             5602 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter baumannii strain TG22182 plasmid pTG22182_2, complete
            sequence.
ACCESSION   NZ_CP039995
VERSION     NZ_CP039995.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10261544
            Assembly: GCF_003949595.2
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Sahl,J.
  TITLE     Genome wide association studies (GWAS) and transcriptomics
            identifies cryptic antimicrobial resistance mechanisms in
            Acinetobacter baumannii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Sahl,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2019) Pathogen and Microbiome Institute, Northern
            Arizona University, PO Box 4073, Flagstaff, AZ 86011, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2019
            Assembly Method                   :: UniCycler v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 01:30:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,915
            CDSs (total)                      :: 3,820
            Genes (coding)                    :: 3,745
            CDSs (with protein)               :: 3,745
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 32 of 75
            Pseudo Genes (incomplete)         :: 39 of 75
            Pseudo Genes (internal stop)      :: 12 of 75
            Pseudo Genes (multiple problems)  :: 8 of 75
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP039995.1.
            Bacteria and source DNA available from Dr. Jason Sahl, Northern
            Arizona University.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="TG22182"
                     /isolation_source="trach asp"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pTG22182_2"
                     /geo_loc_name="USA: Arizona"
                     /lat_lon="34.0489 N 111.0936 W"
                     /collection_date="2011-07-19"
                     /collected_by="LSA"
     gene            1..951
                     /gene="repM"
                     /locus_tag="EA674_RS19725"
                     /old_locus_tag="EA674_019725"
     CDS             1..951
                     /gene="repM"
                     /locus_tag="EA674_RS19725"
                     /old_locus_tag="EA674_019725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001208776.1"
                     /translation="MRELVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHAEGYINQFGVHRNTAYQALKDACNDLFARQFSYQKINERGNIENYRSRWVSEIGY
                     VDNEAVVKLIFAPAIVPLITRLEEHFTKYELQQVSNLSSAYAVRLYELLIAWRSTGSTP
                     IIEVSDFRQRIGVLDTEYKRMERFKTSVLELAIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDNPPIERDPNTLDLFTKMTDAQRHLFANKLSELPEMGRYSQGTESYPQFAI
                     RIAEMLQDPDRIKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            944..1519
                     /locus_tag="EA674_RS19730"
                     /old_locus_tag="EA674_019730"
     CDS             944..1519
                     /locus_tag="EA674_RS19730"
                     /old_locus_tag="EA674_019730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096613.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTPQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATASPQQDATACDTDMQQHATPQQDNDNKKNHELNIAENVAVPQQENTAYHTQTLQH
                     ATLQNVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            1531..1674
                     /locus_tag="EA674_RS19880"
     CDS             1531..1674
                     /locus_tag="EA674_RS19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            1962..3341
                     /locus_tag="EA674_RS19735"
                     /old_locus_tag="EA674_019735"
     CDS             1962..3341
                     /locus_tag="EA674_RS19735"
                     /old_locus_tag="EA674_019735"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_031985417.1"
                     /translation="MLVNFSCENILSFKNEVSFSMLASQKKKDNILTNNFFMAGKEQQE
                     PILETSLIFGANGSGKTNFIATLAYLKRIALNQNPENKFIAPFRLNNDSKKTPSELEIE
                     FYAKNNIKYRYGISIFKGEIIEEWLYYTPQTRETILFHREKNSLIEYNSAGFIEAKDFI
                     DENQKISDKLKNDTAFIFLLSTFNGEHSNAISEWFSNIVILDIEDKKDNLKDTLYYWKD
                     NNDFQNWARPILNSLGICDLKFDHKVESVEDIVKQIKSDQEKLKNSNEKNKELKDKAIN
                     LFDNLLDFVTSSSLEKTEDIDFNSVKVIKNIDGNEFPIPLYLESSGTIKLIHLLSALYR
                     SSKNGEILLIDEFDVKFHTLLSKYIFKKYHDINNKGQVIVTVHDTNLMDTNYFRRDQIW
                     LINKNPKGESELYSLIEFKELASNINNKNYSTEYLTGFFHAIPLFNEDDVDSLMEDNSN
                     G"
                     /name="ATP-binding protein"
     gene            3334..3771
                     /locus_tag="EA674_RS19740"
                     /old_locus_tag="EA674_019740"
     CDS             3334..3771
                     /locus_tag="EA674_RS19740"
                     /old_locus_tag="EA674_019740"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031985418.1"
                     /translation="MAKLRNRGKASNDRSEEQREKNDSNLQTKITLGFDFFLGQDTKGT
                     GQTWEDWHTSGLIVSMLHKLKHLCTLTPAQAKNEGSLKIYGAFPPCSKFKCPSHLKEID
                     SWGTIRQMGNGGKTRIAGFYDKNYVFRIVFLDKNHEFWPTD"
                     /name="hypothetical protein"
     gene            complement(3848..4021)
                     /locus_tag="EA674_RS19885"
     CDS             complement(3848..4021)
                     /locus_tag="EA674_RS19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282482.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQD"
                     /name="hypothetical protein"
     gene            complement(4021..4392)
                     /locus_tag="EA674_RS19745"
                     /old_locus_tag="EA674_019745"
     CDS             complement(4021..4392)
                     /locus_tag="EA674_RS19745"
                     /old_locus_tag="EA674_019745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504219.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWTLMKNGASKDEV
                     RMMQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(4663..4917)
                     /locus_tag="EA674_RS20140"
                     /old_locus_tag="EA674_019750"
     CDS             complement(4663..4917)
                     /locus_tag="EA674_RS20140"
                     /old_locus_tag="EA674_019750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014301.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMKPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     Typing          2..951
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.264"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagagaat tagttgtaaa agacaatgcc ttaattaatg caagctataa cttagattta
       61 gtagaacaac gtttaatttt attggccatt gttgaggcaa gagaaagcgg gaaaggtatt
      121 aatgctaatg atccccttga agtacatgca gaaggctata tcaatcaatt tggcgtacat
      181 cgcaatacgg cttatcaagc attaaaagat gcctgtaatg atttatttgc aagacaattt
      241 agctatcaaa aaataaatga acgagggaat attgagaact atagatcccg ttgggttagt
      301 gaaattggat atgtagataa tgaagcagtg gttaaactta tctttgcccc agccatagtt
      361 cccttaatta cacgcttaga agagcatttc actaaatacg aattgcagca agttagtaat
      421 ctcagcagtg cttatgctgt tcgcttatat gaattattaa ttgcttggag aagtactggc
      481 tctactccta ttatagaggt aagtgatttc cgtcaaagaa ttggcgtact cgatacagag
      541 tacaagcgta tggaacgctt taaaactagt gtacttgagc ttgctattaa acaaattaac
      601 gaacatacag atatcactgt gaagtatgag caacacaaaa gaggtcgatc aatttcagga
      661 ttctctttta cttttaaaca gaaaaagaag gacaacccac cgatagaaag agatccgaat
      721 acgttagatc tctttacaaa gatgactgat gcacaacgcc atctgtttgc aaataaactt
      781 tctgaacttc ctgaaatggg tcgttattcc cagggaactg aaagctatcc gcaatttgct
      841 attcgtattg ctgagatgct gcaagaccct gaccgaataa aagaactata cccataccta
      901 aaaaaagtgg gatatatgcc atcaaataaa aaggacaccg taaatggcta agttatcact
      961 aagtgaagta tctaaaaaat tccatgtgga tagatcaacc atttacagag ctgtacgtaa
     1021 tggacgttta tcacgctcca gtgatggaca attcgatcta gcagaggtca tccgatgctt
     1081 tggagaacct gagcaaacac cccaaaaaat tgaatcatct aagcaagaag gtgatgaatc
     1141 tacaaaaaaa cttattgccc atttagaaaa tgaagtcaaa aaataccaag aacgtgaaga
     1201 acggttaatg caacaaattg accgtatgca aacactcatt gagctgaaaa gtgttgcacc
     1261 tgccacagca tcaccacaac aagatgctac ggcatgcgac actgacatgc aacagcatgc
     1321 gacaccacaa caagacaatg ataataaaaa gaatcatgaa ttaaatattg cagaaaatgt
     1381 ggcagtgcca cagcaagaaa atacggcata ccacacccaa acgctacagc atgccacgtt
     1441 gcaaaatgtg gcagtgccac aacacaaaaa acgtggctta tttggccgtg tgctgaatgc
     1501 tgtttttgat aatgactaag ggaggggatc atgccgaaac tgaaagatat tgccctggga
     1561 attattgtag ctccgctgct gatcccgatc atgctgattg catcgtacca ggataaaaag
     1621 gcgctgaaga aagagctgga tgaatgccaa aaagaaaaag accaggcatc ctgattattc
     1681 aattgagcta atcaaaagtg tggaacattg atgaaataat agcgtggaac gtgaaaagtc
     1741 ataaaaaaag cccgaacagg gatgctcggg ctataaacta gaaactacag cgttgaatta
     1801 taaggataat tatacagcat ttcgtataag gtgtattatg ttaattttag agaaactaca
     1861 aaaacccaat atctacataa accttatttt taaaaaatag cttttttttt aaaaaaaatg
     1921 atttaagatc taattttacg tagccttaat tgaaaaaatt tatgttagta aacttttctt
     1981 gtgaaaatat tctttctttt aaaaatgaag tatcattttc aatgcttgcc tctcaaaaaa
     2041 agaaagacaa tattttaacg aataattttt ttatggctgg gaaagaacaa caagaaccca
     2101 ttctagaaac ttcacttatt tttggagcta atggaagcgg aaagacaaac tttattgcta
     2161 cattggcgta cctaaaaagg atcgctctta atcaaaatcc agaaaataaa tttatagctc
     2221 cttttcgatt aaataatgat agcaaaaaga ctccttctga gcttgaaatt gagttttatg
     2281 caaaaaataa tatcaaatat cgttatggta tttctatttt taaaggcgaa attattgagg
     2341 agtggcttta ttacactcct caaacaagag aaacaatatt attccatcga gagaaaaata
     2401 gtctcataga gtataatagt gctgggttta ttgaagcaaa agactttatt gatgaaaatc
     2461 aaaaaataag tgataaatta aaaaatgata ctgcttttat ctttcttcta tcaacattca
     2521 atggagaaca ttctaatgct atttcagaat ggttttctaa tatagttata ttagatatag
     2581 aagataaaaa agataatctt aaagatactt tgtactactg gaaggataat aatgattttc
     2641 aaaattgggc tagacctatt ttaaactcat taggtatatg tgatttgaaa tttgaccata
     2701 aggttgaatc agtcgaagat attgtaaaac agattaagag cgaccaagaa aagcttaaaa
     2761 atagtaacga aaaaaataaa gagctaaaag ataaagctat taatcttttt gataatcttc
     2821 tagattttgt aacatcatca tctttggaaa aaacagaaga tattgatttt aatagtgtta
     2881 aagttataaa aaatatagat ggtaatgaat tccctatccc tctttattta gagtctagcg
     2941 gtactattaa attaatacat ttattaagtg cactatatag atcctctaaa aatggagaaa
     3001 tattattaat tgatgaattt gatgttaaat tccacacgtt actctctaaa tatatattta
     3061 aaaaatatca tgatattaat aacaagggac aagtaattgt aactgttcat gatacaaact
     3121 taatggatac aaactatttc agaagagacc aaatttggct tataaataaa aaccctaaag
     3181 gcgaaagtga gctatactca ttaatagaat tcaaagaatt agcatcaaat attaataata
     3241 agaactattc aacagaatat ttaacaggtt ttttccatgc tataccacta tttaatgaag
     3301 atgatgtaga tagtttaatg gaagataatt caaatggcta aactgcgtaa taggggcaaa
     3361 gcaagtaacg atagatctga agaacaaaga gaaaaaaatg atagtaattt acaaacgaaa
     3421 attactttag gttttgattt ctttcttggt caggatacta agggaacagg gcaaacatgg
     3481 gaagattggc atacaagtgg acttatagtc tctatgctac ataagctcaa acacctttgt
     3541 actctgacac cagcccaagc taaaaatgaa gggtctctaa aaatatatgg cgctttccct
     3601 ccctgctcta aatttaaatg tccttcacac ctaaaagaga tcgactcgtg gggaactatt
     3661 agacaaatgg gaaacggagg gaaaacccgt attgctggtt tttatgataa aaattatgta
     3721 ttccgaatag tttttttaga taaaaatcat gagttttggc ctacagatta aaatatctaa
     3781 aaatgtacaa gaacacttta ttaagtgttc ttgtacttaa cataaaatct cttatgcgaa
     3841 gcccaggtta atcctgggct ttttgttcaa tctcatcatt ggccgagaac atacttggat
     3901 tcgtaagcct gatattttcc tgacaacttc cacacagcat caatcctgca aaaagttgta
     3961 cattttgtct attttcacaa agcgtacatg gctttgaaag ctgaataata ttatctgaca
     4021 ctactttacc ctcttatagt tatcatctgc atatgaataa cctttagctt gcatcattcg
     4081 aacctcatct ttagaagccc catttttcat taatgtccat aacatcatga aatagagtcc
     4141 agaaaaatct tcatagtatt tttcagaaaa attattatct tcctcaaaat catctattcg
     4201 cttttctaat cgattcacca gatcttcaat acgatctgcc tgctgcttaa cagcaataga
     4261 ttgaaaatca ttagtcaaaa ttttactgaa caatttactg gttctgattc cttctgaatt
     4321 agccaattga tcgtagaatt ctgctacttg tggatctagt ctgactgcat aagttttgac
     4381 tttttcgccc ataaattaca tctcttataa aaattagagt ttgtacaaga ttaaatcaag
     4441 ataattcaat aatttaaatt aaaataataa gtaaattacg tacaaatcta tttgtacaaa
     4501 attaatctat tctaaaacaa aaacttattt aaatataatg ctttttttat gtacaactat
     4561 gagtacatga cttttattaa atttatcaat actttatcta aaaaaagtat ggttttcatg
     4621 tacaagcatg tgcacagcat aaaaaacata taattcaact acttattcta aaaaaagacc
     4681 atttttctgt acacgtttct gagaccctaa aaaatcgctg aaatccttat cctgtaaggc
     4741 tttgaggggt ttcatcgtta gatgcgtggt gtgtgacaaa aaaatcccgg catgtgccgg
     4801 atcttggatt ggaaaattgg ctaaagtgac gtagggccgg tgcttggttt tgcatggaaa
     4861 aaagtattta tttccttgtt tagaaaaacg taaaaaatca attttttgtc cattcatcca
     4921 ggttaaaaat ttcaacccaa aactttaatt atgaaaagct tcacagaaag cgttcaaatg
     4981 cgatttaaga gcctttactt aaaaaacata gatctcatag tgaaaaacag aaaacagccc
     5041 aaaaaacgca aatgagaacg aaataaagag agattttaac tttagataga tgcatagagc
     5101 gagtgtctac gagcgaacta tcaaaatttg cgcctagacc ctctgaaaaa catttttttg
     5161 tcctctttag cctaagaaag cgtaagtttc atacagaaat ttgctcctgg atcgagcgta
     5221 gcgagtaaaa aagttttatg agcgaagcga attccgagtt gcttttgctt tttcttaaag
     5281 tcacgcaagt attaaccaaa aaattgcccc aacgaactga gcgaaagcga agttcaatag
     5341 agtttgagcg aagcgaaaac caagggcaat ttttcattcc ctgggctttt aattattttt
     5401 aatcttttaa atgcttttag acatgctgaa agccttaatt tacaacactt tcaaaggcta
     5461 tatgacacca tttaccttgc aatatgacac cgtttacctt gcaatatgac accgtttacc
     5521 ttgcaatatg acaccgttta ccttgcttat agcactaaaa ttaattattg tgtcatagca
     5581 taataaataa tactggtgct tc
//
