LOCUS       NZ_CP039933            10255 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter baumannii strain TG29392 plasmid pTG29392_3, complete
            sequence.
ACCESSION   NZ_CP039933
VERSION     NZ_CP039933.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10261537
            Assembly: GCF_003949725.2
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 10255)
  AUTHORS   Sahl,J.
  TITLE     Genome wide association studies (GWAS) and transcriptomics
            identifies cryptic antimicrobial resistance mechanisms in
            Acinetobacter baumannii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10255)
  AUTHORS   Sahl,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2019) Pathogen and Microbiome Institute, Northern
            Arizona University, PO Box 4073, Flagstaff, Arizona 86011, United
            States of America
REFERENCE   3  (bases 1 to 10255)
  AUTHORS   Sahl,J.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2018) Pathogen and Microbiome Institute, Northern
            Arizona University, PO Box 4073, Flagstaff, AZ 86011, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 125.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 01:39:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,862
            Genes (coding)                    :: 3,777
            CDSs (with protein)               :: 3,777
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 30 of 85
            Pseudo Genes (incomplete)         :: 55 of 85
            Pseudo Genes (internal stop)      :: 10 of 85
            Pseudo Genes (multiple problems)  :: 8 of 85
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP039933.1.
            Bacteria and source DNA available from Dr. Jason Sahl, Northern
            Arizona University.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10255
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="TG29392"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pTG29392_3"
                     /geo_loc_name="USA: Arizona"
                     /lat_lon="34.0489 N 111.0936 W"
                     /collection_date="2012-03-06"
                     /collected_by="LSA"
     gene            complement(join(10165..10255,1..317))
                     /locus_tag="EA667_RS19850"
                     /old_locus_tag="EA667_019850"
     CDS             complement(join(10165..10255,1..317))
                     /locus_tag="EA667_RS19850"
                     /old_locus_tag="EA667_019850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000792990.1"
                     /translation="MKNLIKGIALTTFAVLLAACSKPDISGVWIPEKVAKDEIFFDYYI
                     IEKKEGSDRYLLKNVTYRIKGGNSYRPMKLPKLIGGQPEKVLEFIKDDTYCVEGSLQTE
                     CVVYTDGKLDFYNQGTFVKSKKNPPEIPVNQ"
                     /name="hypothetical protein"
     gene            complement(317..478)
                     /locus_tag="EA667_RS19855"
                     /old_locus_tag="EA667_019855"
     CDS             complement(317..478)
                     /locus_tag="EA667_RS19855"
                     /old_locus_tag="EA667_019855"
                     /inference="COORDINATES: protein motif:HMM:NF039406.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001983314.1"
                     /translation="MAEKITQIGILVEESLKKDFQAICKAQDKNASQEIRALMREYVKK
                     HRVKNEEN"
                     /name="hypothetical protein"
     gene            1773..2342
                     /locus_tag="EA667_RS19860"
                     /old_locus_tag="EA667_019860"
     CDS             1773..2342
                     /locus_tag="EA667_RS19860"
                     /old_locus_tag="EA667_019860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096622.1"
                     /translation="MAKLSLTEVSKRFKVSRSTVYRAIKEGKLSRSADNQFDLAEVIRC
                     FGEPVIKASPEEQSKAPVKDDADLRQLVDFMKREIESYKEREQRYLDQIDRFQLLLGHK
                     ESSEKVSHDTPVTRSNDTPCDTFNDTPEPIDTQAMIQNETSHDTPLTRLNDAPRDTFET
                     SHDTLKKQPKKRGLLGRVINAFFNDN"
                     /name="plasmid replication DNA-binding protein"
     gene            2373..2708
                     /locus_tag="EA667_RS19865"
                     /old_locus_tag="EA667_019865"
     CDS             2373..2708
                     /locus_tag="EA667_RS19865"
                     /old_locus_tag="EA667_019865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6388 family protein"
                     /protein_id="WP_000064659.1"
                     /translation="MSNGHYTQKALELFKQDPHVQAALNSRVSPEIAKALGESIEELEY
                     RTLIELFRQRADLDNTDTLRLALDMVATPQQKKQALAQYHEDTANALGISLDEYLKIHP
                     QFKAEIE"
                     /name="DUF6388 family protein"
     gene            complement(2821..2967)
                     /locus_tag="EA667_RS20020"
     CDS             complement(2821..2967)
                     /locus_tag="EA667_RS20020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001983317.1"
                     /translation="MKDFFTMVVELTKYLHDRPDILIPLIIIVGVIILIWKGKLKGRIT
                     FGE"
                     /name="hypothetical protein"
     gene            complement(3431..3733)
                     /locus_tag="EA667_RS19870"
                     /old_locus_tag="EA667_019870"
     CDS             complement(3431..3733)
                     /locus_tag="EA667_RS19870"
                     /old_locus_tag="EA667_019870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000702763.1"
                     /translation="MKIRVIRGYVAKYPLEVFYISKDIDCNVIPVAGMMFEDIGLVNAD
                     GQVEAVEITKVTINPVENTYYVELKQNTEQLDKTVLEQKFKNMVADDWEYNEYQF"
                     /name="hypothetical protein"
     gene            3827..4111
                     /locus_tag="EA667_RS19875"
                     /old_locus_tag="EA667_019875"
     CDS             3827..4111
                     /locus_tag="EA667_RS19875"
                     /old_locus_tag="EA667_019875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001071102.1"
                     /translation="MNQEQFQECMKIWIKPDTWHTNHPCDTKRFNQAIQQLMSITGTRL
                     LHPEDFSEQLYIALANEYPKLGQSFIREQVENATQKYDIISSYLYDIRN"
                     /name="hypothetical protein"
     gene            complement(4126..4620)
                     /locus_tag="EA667_RS19880"
                     /old_locus_tag="EA667_019880"
     CDS             complement(4126..4620)
                     /locus_tag="EA667_RS19880"
                     /old_locus_tag="EA667_019880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000999995.1"
                     /translation="MNDSFSCPPNPDVESFLLNKDKGIRFENTSISRTYLIIDDDTGDI
                     LAYFSISFKEITLDTSNISKSLIKRLDGINKRAETLKVYLIGQIGKNFSVKNNPIKLDF
                     ILNEVYNVIAQAQALVGGRVIILECENQPKLIELYERHGFKLIETINGTDSLLTMYTSI
                     I"
                     /name="hypothetical protein"
     gene            complement(4656..4832)
                     /locus_tag="EA667_RS20025"
     CDS             complement(4656..4832)
                     /locus_tag="EA667_RS20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000246872.1"
                     /translation="MATVSFNKSFVIESPTAINAIINDLENPRKVEVSTRDYNAENAKG
                     IKLLKQRLSNFKR"
                     /name="hypothetical protein"
     gene            complement(5114..5326)
                     /locus_tag="EA667_RS19885"
                     /old_locus_tag="EA667_019885"
     CDS             complement(5114..5326)
                     /locus_tag="EA667_RS19885"
                     /old_locus_tag="EA667_019885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000687427.1"
                     /translation="MKIEELDDQELYELAQSVIGCRISLRSSGKVPEDDREDLAMQLQS
                     LFELNRAELIQIILLHSDRYKKENL"
                     /name="hypothetical protein"
     gene            complement(5330..8206)
                     /locus_tag="EA667_RS19890"
                     /old_locus_tag="EA667_019890"
     CDS             complement(5330..8206)
                     /locus_tag="EA667_RS19890"
                     /old_locus_tag="EA667_019890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004837446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000024981.1"
                     /translation="MANYHLSVKTGKNVGSSGSRGASHYAYINREGKYKRDDIEQIESG
                     NLPSWAVDAQHFWKAADKYERANGRIYTELEISLPRELNQEQRQELVQQFVEKTLSKNF
                     TYSYAIHTPLASDGEQNPHVHLMFSERKLDGIDRTEQQFFRRYNPKNPELGGAGKDRYF
                     SARIFVSDVRMEWANHANDYMEKLGLDARIDHRSYQAIGTEIQSQNFRADYVSHDQYSI
                     NENIKNIKRQNGETIIEKPSEVLKVLTATQSVFTMRELDRFLVNHTDGEEQYLKAREAV
                     LMCPEMAVLYGKDSVVLSSNELVGIEESISTLIYNANQDSRIQKPRTLIDKLPLNAVRV
                     AEKRTFNPEQEKAFYTLTSTDRISLLNGSAGTGKSYVLSAVSEAYKDSDYQVYGIALQA
                     ITAKAIASDCDIPSSTIASFLARYESGNLEINDKTVLILDEAGMVGSRDMQKLLMITEQ
                     HNAQLKLVGDSYQLNAVMAGSAFNHIQKNLDHKNIATLENIQRQKTTEMRKASEYLSKH
                     EVDKALDIYQALGKVKACESHEVALAKTIHSWSLDHSESKLILAHSNRDVDELNRMARS
                     ILIKQGKLPAESQKVNTYKGEIDLATGDKIVFKQNNHALNISNGETARIIGFERNAQQQ
                     IKGLMVESDRGGQIKKIDLDSYQHFKHGYANTIHSSQGMTVENAYILASENMNANLTYV
                     ALTRHKGNVELNYSATRFNQEEKIWVRKPDGQYQEKELSAFDNLKRTLGRTEVKNFSTD
                     YSVVQAKDELIKNYLQEHRHSISEKREIYNLNSMLKAMTMPDQHFSKQELIEEVRNSLR
                     SKAILGDEIVKFNGQMNIAKGEQLQLDQELILKTGLFKKEHFEKDTSLQVVDAYRVKDE
                     AIMKARIKGEIYEIPFNKLHIRYSDKYLTEFRQDTSARFDKRFKSELHSKFNQQLQQQR
                     QNQPTQNLNAPNSELKNAPRMKNGPRMS"
                     /name="AAA family ATPase"
     gene            complement(8441..8914)
                     /locus_tag="EA667_RS19895"
                     /old_locus_tag="EA667_019895"
     CDS             complement(8441..8914)
                     /locus_tag="EA667_RS19895"
                     /old_locus_tag="EA667_019895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072639.1"
                     /translation="MSNTQTYGNNNGVELFLVIVGIVIVACLFAIWKATKSLGLDMWTG
                     VHTVGYLIAWLTVMIGIYKIGLNVPNKFLIPVLTMMFFACFLPVLNDYALQASTPSFHM
                     VPQLSYLPEESQKMEFGATKITVWWGMWYAKVIYLAIAGLIGVGIQKAFFNDD"
                     /name="hypothetical protein"
     gene            complement(9074..10156)
                     /locus_tag="EA667_RS19900"
                     /old_locus_tag="EA667_019900"
     CDS             complement(9074..10156)
                     /locus_tag="EA667_RS19900"
                     /old_locus_tag="EA667_019900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000713588.1"
                     /translation="MKKFIYIFLIFFSFLGISSVHAVENYTPGQDLGNPDGSITIPNAA
                     TGVTGKNKVSKEIGNVTQQGMGTLDKLMEAIQSNLEQSLKSSLQNYYQKITAPLIYVFS
                     GFILIWMTFQGFKMMLGMAVDFQSVVSNFIIMMIIWSIVFSWDAFYPYIAEVFLEDIPN
                     LISEMTGHDTQSTFTTFVTIVFDVVTESFKNVDMGITNVVSGFFFVLVYLVLLLEAVIL
                     CVIFFLIWTLCKMVIAILVVVAPIFIAAAMFPATRKYATNWLQAVLTPNAIILLTIVTC
                     DLFLSGVITAYDNVVGENGGSNFVVAFVLMITIGLVIGMFYLIPRIAISLVGSGFEASS
                     SGTQGVMGGFKSGMKKLFSK"
                     /name="type IV secretion system protein"
     Typing          1774..2342
                     /name="rep_cluster_1906"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5334..8206)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9078..10099)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="95.0"
                     /evalue="1.69e-178"
ORIGIN
        1 acacattccg tttgtagtga accttcaacg cagtaggtat cgtccttaat aaattccaga
       61 actttttcag gttgaccacc tattaatttt ggtaatttca ttggtcgata agaatttcct
      121 cctttgattc gataagtcac atttttaagt aaatacctat cagaaccttc tttcttttca
      181 atgatgtaat aatcaaaaaa gatctcatct ttagccactt tttcaggaat ccagacacct
      241 gaaatatccg gtttcgaaca agctgcaagc aatacagcaa aagtagtcag ggcaattcct
      301 tttattaaat ttttcattag ttttcctcat ttttaacacg atgtttctta acgtattcgc
      361 gcattagtgc tcggatctct tggcttgcat tcttgtcctg agctttacag attgcttgga
      421 agtctttttt caggctttct tcaactaaaa ttccaatttg tgtaattttc tcggccatac
      481 accctcaaaa tttcatttaa cgtaggccac aatataattt taatgaggac taatttttgt
      541 attaatttgg ataaaaaccc ttgtaaatca attgttttgt gagtacaaat caataggggt
      601 ataaatagag atgactattt ttcgagtaca gccaatgcaa agcatggcta cgcagaaaaa
      661 tcggcatcac tgaaagttta cataggctct aaaacgcact gttttacgtt ttaagacact
      721 ttgaagttat gaagctaaga aaccatagga aagtttaaaa aactcgaaat acgtcaaaat
      781 agagcgattt aaataggtaa ttttgaatta tctaaagtag ttttgtttga aatcttatta
      841 atctaggaaa ttaaaaaaca atttaaaaaa agcctaagca aataataatt tgtttaattt
      901 aaataatata aatcaaatag ttggcataca tattgcaaaa aagaaaaggc actcaactca
      961 tcctcaacgt atcacacacc cctcgctttg ctcgggctga tggtgaacgt tgagtgagtt
     1021 tcggcaatac tcaccctaag ggcgaccatc taaaaagatg acacatccct taagtattgc
     1081 cgaattaacc taatgggctg acggagccac gcatcggtcg tatccgcacc atgcttcgca
     1141 cacatggcag acaaggacta aaggtcttgc acctttcccc acgcgctcct tgtcgaagta
     1201 tggcgatatc tctgcgaggg tttccccaac atgcaactaa atcaacctat acaactactc
     1261 tcaacgctcc gcttatgtgg ggttcccctg taacggttta gctcgaatct tttgattttc
     1321 actatcgcta cgttgcacca gtgacacttg ttccctccac atgcaacgca tgccaaccga
     1381 aaatcgggca cgcagaagca ggcaaaactt gctgaaagaa gcttatttga tgaaaaaaaa
     1441 cacttaacta acgaagactt atcatttagg gtcttgatct aaacctattt tgctgtgaaa
     1501 atggagttca ggtacaagcc tttggtgaaa tcataacgga ttgccgtcct tatgatcttc
     1561 gttacttgat gttggtagca tcaaataact gcgtaccgac gaatttaaag ccctccttga
     1621 tgtatgtcaa ggagggcttt tcgttttgca ggaaaaaata ttataaaaaa catacccata
     1681 ttgatttgta cacacaaaac aacatgggca tgtttttcgg tgtagaatct cttagaaatc
     1741 atcgaataaa gaattttttc aggatatcgt gaatggcaaa attatcactt actgaggtct
     1801 caaaacgctt taaggtgagc cgttcaactg tttacagagc gatcaaagaa ggaaagctat
     1861 ctcgaagtgc tgataatcag tttgatctgg ctgaagtgat ccggtgcttt ggtgagccgg
     1921 taattaaggc atctcctgaa gaacagtcta aagctccagt aaaagacgat gctgatttac
     1981 gtcagttagt cgactttatg aaaagagaga ttgaatctta taaggaacgt gaacagcgtt
     2041 atttggatca aattgaccgg ttccagcttt tattaggaca caaagagtct tctgaaaaag
     2101 tgtctcatga cacacctgtg acacgttcca acgatacacc atgcgacact ttcaatgaca
     2161 caccagaacc tattgataca caagcaatga tacaaaatga gacgtctcat gatacacctt
     2221 tgacacgtct caatgatgca ccacgagaca cttttgagac gtctcatgac acactaaaaa
     2281 aacaacctaa aaagcgtggc ctacttggtc gcgttattaa tgcgtttttc aatgataatt
     2341 aatctaaaaa tctttataga gaggaaatca ttttgagtaa tggccattac acacagaaag
     2401 ccctagagct ttttaaacaa gatccacatg tacaagcagc tctcaactcg agagtaagtc
     2461 cagaaatcgc caaagcttta ggtgaatcta tagaagaact tgaatataga actctaattg
     2521 agttattcag acaacgtgcc gatttggata acacagatac attaaggcta gctcttgata
     2581 tggttgcaac tccacagcaa aaaaaacagg ctttagcgca atatcatgag gatacggcaa
     2641 acgcattggg aataagttta gatgaatatt taaaaattca tccccaattt aaagctgaga
     2701 tcgaataggg cttaatgtgt catgaaacat catttgagac atctgtaacc attgctatat
     2761 aagcaatgag acaaaatgag acgtctcatg acacacttaa ataaccccat taaaaacaca
     2821 ttactctcca aaagtaatcc gtcctttaag cttacctttc catatcaaga tgattacacc
     2881 aacaataata atgagaggga tcaaaatatc agggcggtca tgtaagtatt tagtaagttc
     2941 aacaaccatt gtaaaaaagt ctttcatttt cgttcaatcc agaaaacttt gaagaactta
     3001 attacactcg tatacgtata tgggttcgtt gtgtgttttc gacacctaat aaacgatacg
     3061 agagtattta aggctataaa taacccttaa attttcggat aaaatgaaaa aaagtatcag
     3121 gcggaaggac ttccgtttat ttttcatatc gaccaaaatt taaagtcgat acctattata
     3181 tagaggaaaa acgcggtaat ttttcaattt tccatatagt gtcaacagga aaaaaatagg
     3241 gagctattct tcaaaatctc taaacaaaat cgtggaacat tgatcaatta ttaagcgtgg
     3301 aacgtgaaaa atcataaaaa aagcccagtc gggctagagt gggctataaa tattcaacta
     3361 cagcgttgaa tttaaaggaa aaaattcaag catttcgtat aaggtgtatt atgttaattt
     3421 tagaaaaact ttaaaattga tattcgttat attcccagtc atccgcaacc atattcttaa
     3481 atttttgttc caacaccgtc ttatctaatt gttctgtatt ttgcttcaac tcaacatagt
     3541 aagtattttc aactggatta attgttacct tagtaatttc tacagcttca acctgtccat
     3601 cagcatttac aagtccaata tcttcaaaca tcattccagc cactggaata acattacagt
     3661 ctatgtcttt tgagatataa aagacttcta atggatactt agctacatat cctcttatta
     3721 ctctaatttt cataaaattc ctttataata agcttgttag tacttaactc tatagatata
     3781 aactatctta ataaatatta ttttcaacag ttaattgaga tcaaaaatga atcaagaaca
     3841 gtttcaagaa tgtatgaaaa tttggataaa accagataca tggcatacaa accatccttg
     3901 tgataccaaa aggtttaatc aggctattca acagttaatg tctataaccg gtactcgact
     3961 tcttcatcca gaagattttt ctgaacaact ttatatagca ttagctaatg agtatccaaa
     4021 actgggccaa agctttatta gagagcaagt cgaaaacgct actcaaaaat acgatattat
     4081 cagtagctat ttatacgata taagaaattg atatttttat aaaaattaaa taatagatgt
     4141 atacatagtt agaagagaat cagttccatt aattgtctca attagcttaa atccatggcg
     4201 ctcatacaat tctatgagtt ttggctggtt ctcacattcc agaataatca cccgtccacc
     4261 aacaagagct tgagcttggg caattacgtt gtaaacctca ttcaaaataa aatctaactt
     4321 aatcggatta ttcttaactg agaagttctt accaatttgg ccgattagat aaacttttaa
     4381 ggtctctgct ctcttattga taccatcaag gcgcttaata agtgatttag aaatattact
     4441 tgtatcgaga gtaatttctt taaaagaaat tgagaaataa gccaatatat cgccggtatc
     4501 atcatcaatg atcagatatg ttcgagaaat agaggtgttt tcaaaacgga tccctttatc
     4561 tttattaagc aaaaagcttt caacatcggg atttggggga caggaaaagc tatcattcaa
     4621 gaactggcaa acatcttcgt caccaagttc ttgaattaac gcttgaagtt ggacaatcgt
     4681 tgttttaata gcttaatacc ctttgcattc tctgcattgt aatcacgagt agaaacctca
     4741 actttacgag gattttctag atcattgatt atagcattaa ttgctgtagg gctttcaatt
     4801 acaaagcttt tattaaacga gacagtagcc attacacggt actcctctag ttctggcaat
     4861 atcatgaagt ttcattaaag ttcattaaac ttcacgaatc ttcattattc gctatagtaa
     4921 atattttcta ttgaaaagta aaattctatt ttatattttc aattgtaagt atctgaaaaa
     4981 taataaaaat gtatttattt atttttgtac tcacaaaaca atccatgcat ctagaaaaag
     5041 ccctatattg ttttgtgcgt acaaaacaat atagggcttt tttatctaaa gaataatttt
     5101 gaaaaacaac aatttataaa ttttcttttt tatatctatc actatgaagc agaataattt
     5161 gaattaactc agctcgattc aactcgaaca atgattgcag ttgcatcgcg agatcctctc
     5221 gatcatcttc aggaaccttt cctgaacttc ttaggctaat ccggcagcca ataacagact
     5281 gtgctagctc atatagttcc tgatcatcca gttcctcaat tttcatgatc taactcatcc
     5341 ttgggccatt tttcatacgt ggggcattct tcagctcact atttggtgca tttaagtttt
     5401 gtgtgggctg attctgtctt tgctgctgta gctgttggtt aaacttgcta tgtagttcgc
     5461 tcttaaagcg tttatcaaaa cgtgctgacg tatcttgcct aaactctgtc aaatatttgt
     5521 ctgaataacg gatatgcagc ttattaaacg gaatttcgta aatctccccc ttaattcgag
     5581 ctttcataat tgcttcgtct ttaaccctat aggcatctac tacctgaagc gaagtatctt
     5641 tttcaaagtg ctcttttttg aataaaccgg tctttaaaat gagttcctgg tctaactgga
     5701 gctgctcacc cttggcgata ttcatttgcc cattaaattt aacaatttca tcccctaaaa
     5761 tggccttgct tcttaggcta tttcggactt cctcaataag ctcttgtttg ctgaaatgct
     5821 gatctggcat agtcattgcc ttaagcatgc tattgagatt atagatttca cgcttctctg
     5881 aaatgctgtg ccggtgttcc tgaagatagt ttttaattaa ttcatcttta gcctgaacca
     5941 ccgaataatc agtagagaaa tttttaactt ctgtacggcc taatgttcgc ttcaggttat
     6001 cgaatgctga gagttctttt tcctgatatt ggccatcagg ctttctaacc cagatttttt
     6061 cttcctgatt gaatctagtc gcgctgtaat tcagttccac attgccttta tgacgggtca
     6121 aagctacata ggtcagattg gcattcatat tctcactggc aagaatatag gcattctcta
     6181 ccgtcattcc ttgtgaggaa tgaattgtat ttgcataacc atgcttaaaa tgctgatagc
     6241 tatccaaatc aatcttcttg atctggccgc cccgatctga ctcaaccatc aagcctttaa
     6301 tctgctgttg ggcattacgc tcaaagccaa taattctggc tgtttcacca ttggagatgt
     6361 ttaaggcatg gttattttgt ttgaatacga tcttgtctcc agtcgcaaga tcgatttcac
     6421 ccttgtaagt atttaccttt tggctttctg cagggagttt accttgcttg atcaggatgg
     6481 aacgcgccat tctgtttaat tcgtccacgt cccgatttga gtgagcaagt atcagcttac
     6541 tttcactgtg atcaagggac catgaatgaa ttgttttggc taaggcaacc tcatgggatt
     6601 cacatgcttt gaccttgcct aaagcctgat aaatatctaa agccttatca acttcatgct
     6661 tagataaata ttctgaagct ttacgcattt cagtggtttt ttgacgctga atattctcta
     6721 gggtcgctat atttttatgg tctaggtttt tctggatatg attaaacgct gaaccggcca
     6781 taactgcatt taactggtag ctatcaccca ctaatttaag ttgtgcgttg tgctgttcag
     6841 taatcatcaa gagtttttgc atatctcttg aaccgaccat accggcttca tccagaatca
     6901 atacagtctt atcattaatt tctagatttc cactttcata tcgagccaag aaactggcaa
     6961 tcgtactact cggaatatca caatctgaag caatcgcttt tgctgtgatg gcctgaagtg
     7021 caataccata aacctgataa tcagaatcct tataagcttc agatacagca ctcaatacat
     7081 aggattttcc tgtacctgct gaaccattca ataaactgat ccggtctgtc gaagttagtg
     7141 tataaaatgc cttttcctgc tctggattaa acgttcgttt ctcagcaacc cgaactgcat
     7201 ttaatggtaa tttatcaatg agtgttcttg gtttttgtat ccggctgtct tggttcgcat
     7261 tgtagatcag tgttgatata gattcttcaa ttccgaccag ttcatttgaa gataaaacaa
     7321 cactgtcctt accatataaa acggccattt cagggcacat caaaacagct tcacgggctt
     7381 ttaaatattg ttcctcacca tcagtatgat taaccaagaa tctatcgagt tcacgcatgg
     7441 taaaaacaga ctgagtagcc gtgagcactt ttaacacttc actcggcttc tcaataatgg
     7501 tttcaccatt ttgacgctta atatttttaa tgttttcgtt aatactgtac tgatcatgac
     7561 taacgtaatc agccctgaag ttctggctct ggatctcggt accaatagct tgataactgc
     7621 ggtggtcgat tcgggcatct aatcccaatt tttccatata gtcgttggca tggtttgccc
     7681 attccatccg gacatccgaa acgaatattc ttgctgagaa gtagcggtct ttccctgccc
     7741 cacctaattc aggattctta ggattgtacc gtctgaaaaa ttgctgttct gtacgatcaa
     7801 taccatccaa ttttcgttcg ctgaacatca ggtgtacgtg tggattttgt tcaccatcac
     7861 ttgctaaagg tgtatgaatc gcataggaat acgtaaagtt tttgcttaaa gtcttttcta
     7921 caaattgttg aactagctct tgtctttgtt cttggtttaa ttctctaggc aaagagattt
     7981 ctaattcagt ataaatccgg ccgttggcac gttcatattt atctgctgct ttccaaaaat
     8041 gctgggcgtc tactgcccat gatggcaagt tacctgattc gatttgctcg atatcgtctc
     8101 tcttatactt accttcacga ttaatatagg cgtaatggct tgcccctcga cttcccgaag
     8161 aacctacgtt tttaccggtt ttaactgata aatgatagtt cgccattttg ccctaattcg
     8221 ataaccgctt tttggtttct cggagagaaa cgcggaagcg tgcattactc attgctattc
     8281 gtaatgatac ccctcaccca tccaattcca gtatttgagt ttcgtaacca gtttctttaa
     8341 aacataggta tctgtttttt tattagaaat ttttttatag atgtaaaaaa accacatatt
     8401 taatgtggtt ttaagtacaa ggttttattg aaaaggcagg ttaatcatca ttaaaaaatg
     8461 ctttttgtat cccaacacca attaatccgg ctatcgcaag gtaaataact tttgcatacc
     8521 acatgcccca ccagaccgta attttagttg ccccaaattc catcttttga gactcctctg
     8581 gaagataaga aagctgtggc accatatgaa aacttggtgt agaagcctgt aacgcataat
     8641 catttaatac agggaggaaa caagcaaaaa acatcattgt taatacaggg attaagaact
     8701 tatttggaac gtttaatcct attttataaa taccgatcat cacggtaagc catgcaatta
     8761 gatacccaac agtatgtacg cccgtccaca tatctaatcc taatgacttc gttgctttcc
     8821 aaattgcaaa aagacaagca actatcacta ttccaacaat aactagaaat agttcaacac
     8881 cattattatt tccgtaggtt tgtgtattac tcatattatc aatcctaatt attaggtttt
     8941 acttcttcgg tcagctcgcc aaaatcacag attttgtcgg cctcgctcaa aataaaacgg
     9001 taatcaaaga gggtaaaaaa ttaaaattta tcagttcgct tcgttaacac ttagctctat
     9061 ttatttcgag ttattatttc gaaaaaagct ttttcattcc agacttaaaa ccacccataa
     9121 caccttgtgt accagaacta gatgcttcga atccacttcc cacaagagag atggcaattc
     9181 ggggaattaa ataaaacatt ccaatgacta ggccaattgt aatcattaaa acaaaagcga
     9241 caacaaaatt agatccgcca ttttccccaa caacattatc gtatgctgtt ataactccag
     9301 ataaaaacaa atcacatgtg acaattgtta gtaaaataat ggcattaggg gtaagtacag
     9361 cttgaagcca attcgttgca tatttcctag ttgcagggaa catggcagca gcaataaaaa
     9421 ttggtgcgac tacaaccaaa atagcaataa ccattttgca taatgtccag attaaaaaga
     9481 aaattacaca taagatgacg gcctcaagaa gtagaacgag atataccaaa acaaagaaaa
     9541 atccagatac gacattggta attcccatat ctacattttt aaaagactct gtcacgacat
     9601 caaatacaat tgtcacaaaa gtcgtaaatg tagattgagt atcgtgccct gtcatttcac
     9661 taattaggtt tggaatgtct tcaagaaata cttccgcaat gtatggatag aatgcatccc
     9721 aactaaatac aatcgaccag atgatcatca tgatgataaa gtttgaaaca acggactgaa
     9781 aatcaacggc cattccaagc atcattttaa aaccttgaaa agtcatccag atcaaaatga
     9841 agccactaaa gacatagata agtggtgcag taattttttg ataataattt tgcaggctag
     9901 acttcagact ctgctctaaa ttactttgta ttgcctccat taatttatct aatgtaccca
     9961 taccttgctg ggtaacatta ccaatctctt tcgatacttt gttctttcct gtaacaccag
    10021 ttgcagcatt cggaattgtt atagatccat caggatttcc aagatcttgc cctggtgtgt
    10081 aattttcaac tgcatgaacg gagctaatac ctagaaaact aaaaaaaatc aggaaaatat
    10141 agataaactt tttcatggta tcacctactg atttactgga atttcaggtg gatttttttt
    10201 tgatttaacg aatgtacctt gattataaaa atctagtttt ccgtccgtat aaacc
//
