LOCUS       NZ_CP039840             6265 bp    DNA     circular CON 14-NOV-2023
DEFINITION  Escherichia coli O55:H7 strain USDA5905 plasmid pUSDA5905_4,
            complete sequence.
ACCESSION   NZ_CP039840
VERSION     NZ_CP039840.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11229503
            Assembly: GCF_013343615.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O55:H7
  ORGANISM  Escherichia coli O55:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Bono,J.L.
  TITLE     Comparison of 54 complete closed STEC O157:H7 genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Bono,J.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2019) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 299.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/14/2023 02:24:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,597
            CDSs (total)                      :: 5,462
            Genes (coding)                    :: 5,177
            CDSs (with protein)               :: 5,177
            Genes (RNA)                       :: 135
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 117 of 285
            Pseudo Genes (incomplete)         :: 153 of 285
            Pseudo Genes (internal stop)      :: 77 of 285
            Pseudo Genes (multiple problems)  :: 55 of 285
            Pseudo Genes (short protein)      :: 4 of 285
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP039840.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Escherichia coli O55:H7"
                     /mol_type="genomic DNA"
                     /strain="USDA5905"
                     /serotype="O55:H7"
                     /isolation_source="Food (meat)"
                     /db_xref="taxon:244320"
                     /plasmid="pUSDA5905_4"
                     /collection_date="1994"
     gene            join(6197..6265,1..930)
                     /locus_tag="E3175_RS26560"
                     /old_locus_tag="E3175_27205"
     CDS             join(6197..6265,1..930)
                     /locus_tag="E3175_RS26560"
                     /old_locus_tag="E3175_27205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234361.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="WP_001082277.1"
                     /translation="MNRTETLKKIQIEVEEFVTYADNEQLIANHDCLTVLKKIPNNSIS
                     LILTDPPYHATQKKNIYGDTQFKKDDEYINWMEEISIEWRRILKPNGSLYCFCDSSMSA
                     RLEVMLSKRFNILSHIVWTKPNQPGFDGWKGKMNKESLRQWYGHSERIIFCEPAKDGNL
                     HRSSFGEFLKKRRKEAGLSGNKLTELTGAYGKVNHGGAVSNWETGRNIPSREQYKKIKE
                     AILNTGKVSFMPEYEDAIRPFIISKNVEYTDVWNFESVRPYKGKHPAEKPQDMLSHIII
                     ASSYEGDIVLDCFAGSGSTALSAKRNKRKCISIDIDTHWTDYIEKRLNELF"
                     /name="site-specific DNA-methyltransferase"
     gene            complement(944..1930)
                     /locus_tag="E3175_RS26565"
                     /old_locus_tag="E3175_27210"
     CDS             complement(944..1930)
                     /locus_tag="E3175_RS26565"
                     /old_locus_tag="E3175_27210"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BstXI family restriction endonuclease"
                     /protein_id="WP_000533217.1"
                     /translation="MGRNIKLPILLSRKIYKTGQTRGADDDVIYQNRVSRNSTVLIPFE
                     LIISAHNRYVLRKNYDNGYIVLIPPDYLFGEMRDETISIMNDCGLVLGVNCLVFYSQRR
                     QYEKYLPDKFGLRYTQPMTRTGDLGGDYVCRISGTTSENMRKISFGYNLTTSKGAGIRL
                     FEYADKEVIEKARIQLEAIYWLCNDSLDAAIEYGMSSEDARERKRLAIDEAKRQGLLNE
                     VELIKNRIIDVDKDTVCPLCLAKISAKGFFSKVEQQEFRRVHDLTVTEVNLFHINELRY
                     GEFNHKPYNLGWGHHHCNVVVKDSGIDNTLEWMHEILVRNERLNKAP"
                     /name="BstXI family restriction endonuclease"
     gene            complement(1914..2162)
                     /locus_tag="E3175_RS26570"
                     /old_locus_tag="E3175_27215"
     CDS             complement(1914..2162)
                     /locus_tag="E3175_RS26570"
                     /old_locus_tag="E3175_27215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107566.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_071529142.1"
                     /translation="MENLLLSKISFVIKKIRLEKGMTQEDLAYKSNLDRTYISGIERNS
                     RNLTIKTLGLIIKGLGVSNIVFFELLVKEISTHGQEY"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(2823..3056)
                     /locus_tag="E3175_RS26575"
                     /old_locus_tag="E3175_27225"
     CDS             complement(2823..3056)
                     /locus_tag="E3175_RS26575"
                     /old_locus_tag="E3175_27225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137440.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3063..3593)
                     /locus_tag="E3175_RS26580"
                     /old_locus_tag="E3175_27230"
     CDS             complement(3063..3593)
                     /locus_tag="E3175_RS26580"
                     /old_locus_tag="E3175_27230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001483786.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTAALKELLERYQKTTVSTMDTHWQTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINENMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3631..4281)
                     /locus_tag="E3175_RS28285"
                     /old_locus_tag="E3175_27235"
                     /pseudo=""
     CDS             complement(<3631..4281)
                     /locus_tag="E3175_RS28285"
                     /old_locus_tag="E3175_27235"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4271..4594)
                     /locus_tag="E3175_RS26590"
                     /old_locus_tag="E3175_27240"
     CDS             complement(4271..4594)
                     /locus_tag="E3175_RS26590"
                     /old_locus_tag="E3175_27240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001483787.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQRVQ
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4659..4850
                     /locus_tag="E3175_RS26595"
                     /old_locus_tag="E3175_27245"
     CDS             4659..4850
                     /locus_tag="E3175_RS26595"
                     /old_locus_tag="E3175_27245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5117..5425
                     /locus_tag="E3175_RS26600"
                     /old_locus_tag="E3175_27250"
     CDS             5117..5425
                     /locus_tag="E3175_RS26600"
                     /old_locus_tag="E3175_27250"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001292104.1"
                     /translation="MSELEMSLTELITTIVQDPLFKVKAAGKALLNQNDGYHILMAIHE
                     HGEQAVQIEMAKQIAAREKMSFTEAARKASYYIEYAVMASNGDGYGKTTRNELNSKG"
                     /name="hypothetical protein"
     gene            5759..5902
                     /locus_tag="E3175_RS28460"
     CDS             5759..5902
                     /locus_tag="E3175_RS28460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_237442139.1"
                     /translation="MHEPPVQPDRCVLSGNYRLESNPVKHDLTPLAAATCNRISRARFI
                     SW"
                     /name="replication initiation protein"
     Typing          complement(4929..5007)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.593"
                     /coverage="90.0"
                     /evalue="7.01e-24"
     Typing          5505..5868
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.986"
                     /coverage="89.0"
                     /evalue="1.26e-172"
     Typing          complement(2720..4281)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.545"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gagcaattaa tagccaatca tgactgttta actgtattaa aaaagatccc gaacaattca
       61 atttctttga tacttaccga tccaccatat catgcaacac agaaaaagaa tatttatggt
      121 gatactcaat tcaaaaaaga cgatgaatat ataaattgga tggaagaaat ctccattgaa
      181 tggcgaagaa tattaaaacc aaatggttct ctttattgct tctgtgattc atcaatgtct
      241 gcacgattag aagttatgct atctaaacga tttaatatat taagtcatat tgtatggact
      301 aaaccaaacc aacctggctt tgatggttgg aaagggaaga tgaataaaga atcattacgt
      361 cagtggtatg gacattcaga aaggattatc ttttgcgaac ctgcaaaaga cggaaattta
      421 catcgttctt cttttggtga attcttaaaa aaaagaagaa aagaagcagg tcttagcgga
      481 aacaagctaa ctgaattaac aggcgcttac ggaaaagtta atcatggcgg cgctgtatct
      541 aactgggaaa cgggaagaaa tatacctagt cgcgaacaat ataaaaaaat aaaagaagcc
      601 atattaaata cagggaaagt gagcttcatg cctgaatatg aagatgcaat acggcctttt
      661 attatttcaa aaaatgtaga atacactgat gtttggaatt ttgagtctgt tagaccatat
      721 aaaggaaagc atccagcaga aaaaccacaa gatatgttaa gtcatattat aattgctagc
      781 tcctatgaag gcgacatagt tttagattgt tttgctggtt ctggtagcac cgcattatca
      841 gcgaaaagaa acaaacgaaa atgcatatct attgatatag atacgcattg gacagattat
      901 atagaaaaaa gattaaatga actgttttaa acgcagagcg cctttatggc gctttattta
      961 acctttcatt tctaaccaaa atttcatgca tccactctaa tgtattatca atgccagaat
     1021 cttttaccac cacattacaa tgatgatgcc cccaccctaa attatatggt ttatgattaa
     1081 actcaccata ccttaactca ttgatatgaa aaagatttac ttctgttaca gtaagatcat
     1141 gtactcttct aaactcttgc tgttctactt ttgaaaaaaa acctttagcc gatatctttg
     1201 ctaaacataa tgggcaaaca gtatctttat caacatcaat tattctattt tttatcaatt
     1261 caacttcatt taatagccct tgtcgttttg cctcatcaat ggctaatctt tttctttccc
     1321 tagcatcttc agatgacatt ccatattcta tagcggcatc taatgaatca ttacacaacc
     1381 aatatattgc ctcaagttga atacgcgcct tttctattac ttctttatcg gcatactcaa
     1441 acagtctaat tcctgccccc ttagatgttg taagattata cccaaatgat atttttctca
     1501 tattttcaga agttgttcca gaaattctgc aaacatagtc accaccaaga tctccagtcc
     1561 gagtcattgg ctgggtatac ctaagcccaa atttatctgg gagatatttt tcatactgac
     1621 gcctctggct ataaaaaacc aagcagttaa cacctaaaac taatccgcaa tcattcataa
     1681 tagaaatggt ttcatcacgc atctctccaa ataaataatc aggcggaatt agaacaatat
     1741 aaccattatc atagtttttt cttagcacat atctattatg cgcagatatt atcagctcaa
     1801 atggtattaa aacggtgcta tttcttgaaa ctctattctg gtaaattaca tcatcatcag
     1861 ctccgcgagt ctggcctgtt ttgtatattt ttcgcgataa taaaatagga agcttaatat
     1921 tcctgcccat gtgttgaaat ctcctttaca agtagttcaa aaaaaacaat atttgatacc
     1981 cccaagcctt ttataattaa cccaagagtt tttattgtta aatttcttga attccgttca
     2041 atcccggaaa tataagttct atctaagttt gatttatacg ccagatcttc ttgagtcatt
     2101 cctttttcta gtcttatttt ctttattaca aaagatattt ttgataaaag aagattttcc
     2161 ataactaata ttttttgttc gtttttggtg gtatcatcat gcctccatgt cgactatagt
     2221 tacacggact ataagtcaca tcattaattc attaatacgg caaattccat ttaccataac
     2281 gtcgcattgt acgcaccact aaaacgcgtt cggtgcgatc acggcagcag acaggtaaaa
     2341 atggcaacaa accacccgaa aaactgccgc gatcgcacca ggtaaatttt aaccacatgc
     2401 atagctatgc agccatgtga atcacgcagg aatttccagc cagagactgc tgaaacggat
     2461 ttttttcata ctttcggaaa ggcagaagac ggaaccggag cggaaaaaca gaaattcaga
     2521 ggaatgagcg cgatctggca ataacggcaa aacacagcaa caaaagatac accagaatcg
     2581 cgcccgtatg cgttttaacg cgttcctgtg ttttcagcaa ggtctgactg aagcgcgttc
     2641 ccggtgcccc cgaaccaacc attatcccaa aaaatgcacc ggaaacgcgc tgacaacatt
     2701 ttagcctgtc tttggttacc atcccagcga agggccatcc agtgtgcgtt cctgtatttc
     2761 cggctggcgc tcacgctcca gggataacgc atgttcgcgc tcctgtatca gccgttcgtc
     2821 ccttatctcc tgttctcgct gtataactgg ctcaagcgtt ctgtccgctc gctcaagtgc
     2881 tgcacctgct gactcaactg catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta
     2941 agcgaaaaca ttttctgcaa ttccacgaag gcgctctccc attcgttcag ccgctgcata
     3001 tagtcctgtt gcagttgctc taaggcgttc agcaaatgtc tttccagctc cgtcatgctc
     3061 tgtcactcct gcaccagtct cactgcgttc agtttgccgt tgtccttcgt ccagttcgtt
     3121 tcggctttca tccccttcgg caacaccaga aaacgtccgt tgctgtcctc gtgataacgc
     3181 acgccccatg tcttcgcgtt cagcttctcc agactctctt tctgctcgcg gagaatattc
     3241 atattctcgt ttatcttcat gccctggtac cacagcagac tcccggtcgt ggcaaacatc
     3301 agtactgaga cgataatcag ccacagccag cgtttcacgc tcattttcag cacggtttgc
     3361 cagtgtgtgt ccatcgtgct gacggtcgtt ttctgatacc tctccagaag ttctttcagg
     3421 gctgctgtat ggtcgttgat agcgttcctg attaaagatt cgtttgaggt cagttccctg
     3481 tttacagatt cttcgagctt cctgaactcg tcgttcagca ttgtctctgt agattcggct
     3541 ctctctttca gctttttctc gaactccgcg cccgtctgta aaagattgct cataaaatgc
     3601 tcctttcagc ctgatattct tcccgccgtt cgggtctgca atgctgatac tgcttcgcgt
     3661 caccctgaca acttcaagcc ccgcctctgt gagcgcctga atcacatcct gacggccttt
     3721 tatctccccg acatgataaa gagcgtctat cccccgtgta acgctctcag caagcgcctg
     3781 tttcgtttcc ggcaggttat cagggagagt cagtatccgg cggttctccg gggcgttggg
     3841 gtcatgcagc ccgtaatggt gattcaccag cgtctgccag gcatcaattc tcggcctgtc
     3901 tgcccggtcg tagtacggct ggaggcgttt tccgctctgt agctccatgt tcggaatgac
     3961 aaaattaagc tcaagccgtc ccttgtcctg gtgctccacc cacaggatac tgtactgatt
     4021 cttttcgaga ccaggcatca gtacgcgctc aaagctcgcc atcacttttt cacgtcctcc
     4081 cggcggcagc tccttctccg cgaacgacag aacacctgac gtgtatttct tcgcaaatgg
     4141 cgtggcatcg atgagttccc ggacttcttc cggattaccc tgaagcaccg ttgccccttc
     4201 ccggtttcgc tcccttccca gcaggtaatc aaccggacca ctgccaccac cttttcccct
     4261 ggcatgaaac ttaactatca tcctgcactc tctgttccct gaccacctgc cgtaagctgc
     4321 gcaactctct ctcgatggcc atcagtgcgg ccaccacctg aacccggtca ccggaagacc
     4381 actgcccgct gttcaccttc ctcgcagtct gattcaggtt attcccgatg gcggccagct
     4441 gacgcagtaa cggcggtgcc agtgtcggca gcctcccgga gcgggcaacc ggctcaccca
     4501 gacagacccg ccgcatccat accgccagct gtttaccctc acagcgttca agtaaccggg
     4561 catgttcatc atcagtaacc cgtattgtga gcatcctctc gcgtttcatc ggtatcatta
     4621 cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg
     4681 cccttaacat ggcccgcttt ataagaagcc agacattaac gctgctggag aagctcaacg
     4741 aactggacgc agatgaacag gccgatattt gtgaatcact tcacgaccac gccgacgagc
     4801 tttaccgcag ctgcctcgca cgcttcgggg atgacggtga aaacctctga cacatacagc
     4861 tcccggagac agtcacagct tgtctgtgag cggatgccgg gagcagacaa gcccgtcagg
     4921 gcgcgtcagg gggtttcggg gcgaagccct gaaccagtca cgtagcgata gcggagtgta
     4981 tactggctta gtatgtcaga atcggtggca gtggtgcgaa aatttagagc ggatttaagg
     5041 cgtttgggaa gggcataatg ataaagcttg aattaagggt gttagccaat atattgaagg
     5101 attaccgtcc acatatatga gtgaactgga aatgagtctg acagaactaa ttacaacgat
     5161 agtacaagac ccactgttta aagttaaagc agcagggaaa gcgttattaa atcagaatga
     5221 tggctatcac atactaatgg cgatccatga gcatggtgag caagcggtac agatagaaat
     5281 ggcaaagcag atcgcagcta gggaaaagat gtcatttacg gaagcagcga ggaaagcatc
     5341 ctattacata gaatatgctg tgatggcttc taatggtgat ggatacggaa agacaactcg
     5401 aaacgagtta aattcgaaag gttagttcca aaaaccatat ataagatcag ccgagacagg
     5461 ctgtgatctt atgtaggcta actagtgata ggagcctttg ccgtttttcc ataggctccg
     5521 cccccctgac gagcatcaca aaaaccgacg ctcaagtcag aggtggcgaa acccgacagg
     5581 actataaaga tcccaggcgt ttccccccgg aagctccctc gtgcgctctc ctgttccgac
     5641 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5701 tagctcacgc tgttggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5761 gcacgaaccc cccgttcagc ccgaccgctg cgtcttatcc ggtaactatc gtcttgagtc
     5821 caacccggta aaacacgact taacgccact ggcagcagcc acttgtaacc ggattagcag
     5881 agcgagattt ataagttggt aacagagttc ttgaagtatc agccatattc tggctacact
     5941 agaaggacaa gttttaggta tctgcgctct gctgaagcca gttacctcgg ttagataatc
     6001 tgccaggatt aactaacctt cgaaaaaacc acctgccagg gtggtttttt cgtttacata
     6061 gcaagagact acatctcact gcttaggatc tcaagaggat ccgctctaca gaacttatct
     6121 atcaaataat taaaagattg aaaaaaaaac cttggatgtt agaatgttga ctatagtcaa
     6181 caaaagaaaa atggttatga acagaactga aactttaaaa aaaattcaga ttgaagtcga
     6241 agagttcgtc acttatgcag ataat
//
