LOCUS       NZ_CP039812            11970 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain C2660 plasmid pC2660-5, complete
            sequence.
ACCESSION   NZ_CP039812
VERSION     NZ_CP039812.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11479264
            Assembly: GCF_010183645.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Gao,H., Liu,Y., Wang,R., Wang,Q., Jin,L. and Wang,H.
  TITLE     The transferability and evolution of NDM-1 and KPC-2 co-producing
            Klebsiella pneumoniae from clinical settings
  JOURNAL   EBioMedicine 51, 102599 (2020)
   PUBMED   31911273
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Wang,R. and Gao,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2019) Department of Clinical Laboratory, Peking
            University People's Hospital, Xizhimen South Street, Beijing 100044,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 17-DEC-2018
            Assembly Method                   :: Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage :: 240x (PacBio) + 200x (Illumina)x
            Sequencing Technology             :: PacBio RSII + Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010183645.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 02:44:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,941
            CDSs (total)                      :: 5,828
            Genes (coding)                    :: 5,667
            CDSs (with protein)               :: 5,667
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 62 of 161
            Pseudo Genes (incomplete)         :: 107 of 161
            Pseudo Genes (internal stop)      :: 19 of 161
            Pseudo Genes (multiple problems)  :: 26 of 161
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP039812.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C2660"
                     /isolation_source="pus"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC2660-5"
                     /geo_loc_name="China:Beijing"
                     /collection_date="2017-08-01"
     gene            complement(join(11260..11970,1..21))
                     /gene="mobC"
                     /locus_tag="FA957_RS30195"
                     /old_locus_tag="FA957_30370"
     CDS             complement(join(11260..11970,1..21))
                     /gene="mobC"
                     /locus_tag="FA957_RS30195"
                     /old_locus_tag="FA957_30370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(21..1985)
                     /locus_tag="FA957_RS30200"
                     /old_locus_tag="FA957_30375"
     CDS             complement(21..1985)
                     /locus_tag="FA957_RS30200"
                     /old_locus_tag="FA957_30375"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3469..3618)
                     /locus_tag="FA957_RS30205"
                     /old_locus_tag="FA957_30385"
     CDS             complement(3469..3618)
                     /locus_tag="FA957_RS30205"
                     /old_locus_tag="FA957_30385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3703..3960)
                     /locus_tag="FA957_RS30210"
                     /old_locus_tag="FA957_30390"
     CDS             complement(3703..3960)
                     /locus_tag="FA957_RS30210"
                     /old_locus_tag="FA957_30390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3970..5655)
                     /locus_tag="FA957_RS30215"
                     /old_locus_tag="FA957_30395"
     CDS             complement(3970..5655)
                     /locus_tag="FA957_RS30215"
                     /old_locus_tag="FA957_30395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(5760..7247)
                     /gene="ltrA"
                     /locus_tag="FA957_RS30220"
                     /old_locus_tag="FA957_30400"
     CDS             complement(5760..7247)
                     /gene="ltrA"
                     /locus_tag="FA957_RS30220"
                     /old_locus_tag="FA957_30400"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            7892..8137
                     /locus_tag="FA957_RS30225"
                     /old_locus_tag="FA957_30410"
     CDS             7892..8137
                     /locus_tag="FA957_RS30225"
                     /old_locus_tag="FA957_30410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8411..8782
                     /gene="tolA"
                     /locus_tag="FA957_RS30230"
                     /old_locus_tag="FA957_30415"
     CDS             8411..8782
                     /gene="tolA"
                     /locus_tag="FA957_RS30230"
                     /old_locus_tag="FA957_30415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            8779..9144
                     /locus_tag="FA957_RS30820"
     CDS             8779..9144
                     /locus_tag="FA957_RS30820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            9319..9597
                     /locus_tag="FA957_RS30240"
                     /old_locus_tag="FA957_30425"
     CDS             9319..9597
                     /locus_tag="FA957_RS30240"
                     /old_locus_tag="FA957_30425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            9585..9899
                     /locus_tag="FA957_RS30245"
                     /old_locus_tag="FA957_30430"
     CDS             9585..9899
                     /locus_tag="FA957_RS30245"
                     /old_locus_tag="FA957_30430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            10063..10491
                     /locus_tag="FA957_RS30250"
                     /old_locus_tag="FA957_30435"
     CDS             10063..10491
                     /locus_tag="FA957_RS30250"
                     /old_locus_tag="FA957_30435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            10517..10696
                     /locus_tag="FA957_RS30255"
                     /old_locus_tag="FA957_30440"
     CDS             10517..10696
                     /locus_tag="FA957_RS30255"
                     /old_locus_tag="FA957_30440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(10723..11253)
                     /locus_tag="FA957_RS30260"
                     /old_locus_tag="FA957_30445"
     CDS             complement(10723..11253)
                     /locus_tag="FA957_RS30260"
                     /old_locus_tag="FA957_30445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..10655
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2788..3366
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.454"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11264..11970)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.068"
                     /coverage="97.0"
                     /evalue="1.42e-136"
ORIGIN
        1 gttgtactgc tccagtgtca tcagtaaagc cccgcaaaat catcatccgc ttttggtccg
       61 ccgccgtcgg ccggaggcag gtactccgcc agcgggtccg ttttcgccag caccgggaag
      121 gttttcagcg aaatttcccg gcgctcaacc cgcagcgggc tgctgaacgc cagccgcgcc
      181 acgcccagcc cggtcatcac cgcgaacccg tcaggcaggt gttgcagggt gttcacgtca
      241 aacagcggcc gcgcctcctt ctggatgaag ctgtcgccgc tgatgtgctc cacgttcccc
      301 gcctcccggc tggcccggcg gcgctccacg tccacccgga tctccccggt ctggcccgcc
      361 gcccactgcg cggactcctg gctggccgcg cgatagaacc agcggatcgg cgtgttatcc
      421 agtaccgtgg ttttcacaaa atcagcgggc agatcctgcc cgcactgacc gaagtcgccc
      481 agcgactggt gcgcgagcag caggttgcag tcccggctgc gcagcgtccc cagcgcgttc
      541 agcacgtact tcgacagcag atatttaatc tcatcgagca tgatgctcgc gtgcggccac
      601 ctccggaact catcccgcgc aatgatgatt tgcgcgcagc gggcaaacag catcttctgc
      661 acccggatca ccgcctcatc gtccatcgag ccgatgacgt acaggcagcc gccgccgttg
      721 attatccccg ccacatcgat accgctgtcc gtctgcagga cgctcaggtc ggccacgttc
      781 tccagcttgg tgatcagccc cttcacgtcc tttttcaggg cctccggcaa cgcgtacgcc
      841 gccgccagca cctgctgaat gtttgccccc tgcgggaact gctccgcaat cagcttcgcc
      901 gctttctgct cgctgatgcg gtagtggtcc gccacgtcgc cggtctcact caggttaaac
      961 ccggcaacca gcagattctt cagggcgtac tggtcgagat cgcggaacag attcagctgc
     1021 gccggtttcc ccggccgcag atccagcagg gtgaagtccg ggcagtgcgc ccggaagacg
     1081 tgcggcgccc acgcgtcccc tttggggtcg aagtacacca cagcgtcccc gaaatcgcgt
     1141 acgcagcgaa tgagcgcgtt ggtggccatc gcgctcttgc cgctcccggg caggccgagg
     1201 atctgaatat tggtcttgcg ccaggtatca cgagggacca gcaccggctg gccctgctca
     1261 tccttaccca gaaacaccgc ctgctgtttc cgggcctgct ccgtggaaaa atcggccagc
     1321 gcgttaaccg gtaccggctc cccgaaatgc aggtggcgga cgtctgtcat ttctccggtc
     1381 cgctggctgc tgcgccggct ctcctgattc agtttcgcgt tcaccgcctc gtcagcggcc
     1441 gcaaaatgac catcaagcag caggaacgcc agcagccgcg ccagaaggac gccggcgaca
     1501 agccctccgg cacaccaggc tatcccgaac aggttagccg gctgcagcag gtaataatct
     1561 gcgttcgcat agaggtccgg gcgcaggacg gtttctcccc agctctcgat aaacacggcg
     1621 acaaccagcc cccccagaag ggccagaccg gcagcgccgg tcagccagta cgcgtagttc
     1681 cagacatgtc gccgtgccct gaccatccgc ccgggtgaat ccagcgaggc cagcaccgaa
     1741 aatggcacag tccggttatt atcgcgctta tagcgatcgg catactcccg ccgcgcctta
     1801 tggctcagcc ccacaaaacg cattcggccg gcccagagtt cagccagacc gaaaaacagg
     1861 ccgataacca gaaaaaaacc ggccagactg taatactccc ggtacagcca gctgacccac
     1921 gggtaaatct gccgggccag ctccgggata tgccactgcc aggcaagcga atccgtatta
     1981 aacatgcaat ctctctcctt cagacgacgg gtttaccggc gtgatgcgca gcccccgctg
     2041 cacaagccgc ggacatgccc caaggcatgg cggcggcggt cacgtccgtg gtgattgcgt
     2101 tcagctttca ccgcggaggc cagcctcttt ccgccccatg cggaaaaggc gcagccccgg
     2161 aggaacaacg agcacgcccc agcgtgtgag aggaaaaggc ggcaccccag tgcggtcttt
     2221 tccggtgttc cggcccccgt cagagttcgc catagcgagc ggctgaccac ccgcaaaatc
     2281 aggagcagct ttgctgcttc gtcaggtgcg tccagcaccg tggatttttg tgggtggtca
     2341 ggcgtgagcc tgacgggggc cgggtgaatt tctggcgtgg ccggcgtcca gccggacaga
     2401 gccagaaaga ggggaaaaga ccgtgcgtcg gtggcgtcca gccagcgtaa gcaaggtctt
     2461 ttcccctgaa gtcagccacg ccccagcggg gatgacttgc atttctccgc aaggagaaaa
     2521 aagcaacaga gataggttaa cgtaaaatgt tcagcgaaaa accgacatgg ttcacctatc
     2581 ctgataattg atcgtcaggg aatagaaaga cttaatcagg ggacaatatc ccacatcagc
     2641 gcgtccggtg accgtcttgc aggtgccggg gaaattgcgc ctcctcgctc actcggtcgc
     2701 tacgctccgg gcgtgagact gcggcgggcg ctgcggacac atacaaagtt acccacagat
     2761 tccgtggata agcaggggac taacatgtga ggcaaaacgg cagggccgcg ccggtggcgt
     2821 ttttccatag gctccgccct cctgccagag ttcacataaa cagacgcttt ttcggtgcat
     2881 ctgtgggagc cgtgaggctc aaccatgaat ctgacagtac gggcgaaacc cgacaggact
     2941 taaagatccc caccgtttcc ggtaggtcgc tccctcttgc gctctcctgt tccgaccctg
     3001 ccgtttaccg gatacctgtc cgcctttctc ccttgcggga agtgtggcgc tttctcatag
     3061 ctcacacact ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa
     3121 gaactccccg ttcagcccga ctgctgcgcc ttatccggta actgttcact tgagtccaac
     3181 ccgggaaagc acgcaaaacg ccactggcag cagccattgg taactgggag ttcgcagagg
     3241 atttgtttag ctaaacacgc ggttgctctt gaagtgtgcg ccaaagtccg gctacactgg
     3301 aaggacagat ttggttgctg tgctctgcga aagccagtta ccacggttaa gcagttcccc
     3361 aactgactta accttcgatc aaaccacctc cccaggtggt tttttcgttt acagggcaaa
     3421 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct actgaaccgc
     3481 gatccccgtc agttcagagg aggacgatgg tgccaccgtt ccgccctgaa catcccggat
     3541 ataatttgcc tgacatgcgg ccagaagaaa gccagcaaca atcaatataa aaagaaaaat
     3601 agtctttacc tttttcatat actttccctg tatttaaaga ctccggcatt catccctgaa
     3661 caccggaagt cagtatttca aatcatcctt gataattttt tgttaccagt taccgtcccg
     3721 ataatcaaaa gctatataac aattatccac atcaggtttt atttgatttt taaaaaatgg
     3781 ctgtagtaaa ggaacccatt ctttcttcaa atcaaactca ccattattaa tattatcctt
     3841 taaaggcatt cccaagcttt caatgacaga accatcgtca ccaaagtctt tagaatactc
     3901 ttccccttta aactcttcag ttttcttatc aaaccatgtc aaacgtaatt taagccccat
     3961 atttacctct caaagatatt ttttaatgtt ccgttttgga tctggccctt taacctgctt
     4021 acccgttttg ggatcaaatg cgcctaggtg ttcgccatca ctggcacgat acccttcaag
     4081 ctcaccgtgc tgggaatccc attcataaat tttacgtttt ttatcgccat accatcgggc
     4141 ccgcttacca ccaccacctt gtttaggtgt tttaggatca cctcgcttta actctccaag
     4201 tccctttatg tcttctgttt taggtactgg atgataatca tggccgtagt ctttagtccc
     4261 cttgcgaggc ttgttctttt cctcatttaa cttattttct gcatccttcg cttttttctc
     4321 cttttgtttc cggctctcca tcgccccggt aagtgcagcc ttatctctgg ataaggattc
     4381 attcattgcg tttaaagatg cctgagccgc ttttaatttc tcattctgat tctgaatatc
     4441 cttatcaaga tagcgaacct gatcctgcca cccggagcca tattcatgag gcgggttata
     4501 agtcacaaaa tcatctttat ttgcctctgc ctcttttacc tttttatcgg cttcctgaac
     4561 agcagccctt gcaccgggga gcgccttctc tgtcgatgca atgcggctgt ttaaggtcgc
     4621 aatattttta tcttcggcat ccagctcagc tttcgcttta tcatagtctc ttttcagccc
     4681 ctcttccgga tgagcggcgt cccatgcctg ctggcgacgc ttttcttcct cctgacgctg
     4741 ttttacctgt gccggggtaa gaacatctgt caccgacaca taaaccggct tctgtccggt
     4801 ctctttcggg aaacgaataa cggcctcacg ggagttacca ccggctgtaa aaccagccgg
     4861 acgtgaatca cctttttcag caacaatgcc ttttggcggg gctttcgctg tcggaacacc
     4921 tttcggtacg ctcacctgca gagacgggag acccggaatc gacacactga atacccccgg
     4981 acgttttgtc ggtttcgcat ccaccacagg gacactcatt ggccgacctg cgacaaccgc
     5041 aatatgctgc cgctcatcct tcaccacatc cacaacccgt tgtctgacac gaaccgtcgc
     5101 ctggtccagt ggtaaagtac ttgccggagt ctccgtcacc gcatcggccg gtaatgacgt
     5161 cataattttt gacatcattt tcggatcatc ttttgcaatc tcagaaggca gcacaccgta
     5221 gatcgcaatc ccccacagac caaacttaaa cggccccttc agggcagcca gcacatcagc
     5281 aacggcggcc gacaacgcat cgccggaaac tgataaagcc agtccttcag cccccggagt
     5341 agccagggca ggcagaccga aggccatcgc agtggccgaa gactgtccgc caccagagct
     5401 accgctatga ttgccgctgt taccaccatt accaccgggt ttactcccgc cgttaccaaa
     5461 ctcccccgga ttatagttat gaatatctcc attcggcgta tgcgtcgtac cccagcccgg
     5521 cccgctgttt ggatccgtac caccacctga tgaagggcca cctttaccag acgtaccgtt
     5581 cacattccca ctggcctgac caccattatg tcccagacct gaattacccg gacctcgacc
     5641 gtctccacca ctcataccgt tttcctcttt gacatttaaa acaataaatt aaagccaaca
     5701 tgtcgcggta gggatacccg ttaccggata ccccccgcac agatcccggc gtgcgcgatt
     5761 tacgcaccgg gctcctgcct cgggtgtctg gcggtgaacc gctccacagg ccatggatga
     5821 agaacccgaa cccttggtag ccatgcggct gccagtttgt ttgctttcgt ccaggtcgta
     5881 tcatccttct ggctcctgcg cctgagcgcc cggcgccaga ggtttgttac gtgtgtcctg
     5941 aacttctgca tggtggggaa gttgcccggt accgagtgat agttcaggta tccctgaacc
     6001 actctcctga gccattttcc ctgttcgggg attgagtaat gccagcgcct tcgcagaccg
     6061 tctttgatgg ctttcagagt tgccgtcatc cgatcccggc gggtctttcg tatcagcatg
     6121 aacctgccgt tgcgatcttt cccgctgatg tgcgtgaacc cgaggaagtt gaacgtttct
     6181 ggtttgcctt ttcccctgat ggcacggttt tcggcagcga agcggccgaa ctccatcaga
     6241 cgggttttct ccgggtgaac cgtgagtccg aactccctca gtctgcgctg catggctata
     6301 cggaagcgcc gggcatcgta tcgtttgtcg aacccgatga cgatgtcatc ggcgtatctg
     6361 accattacca cattgcctgt ggcatagcga cgtcgccact gatgcgccca cagatcgaag
     6421 acgtagtgga ggtatatgtt tgccagcagc ggtgagatga ccgcaccctg tggggtgcct
     6481 tcctccgttg ctcgccattg accctcctcc gacgtcccgg ctgtgagcca cttacgtatg
     6541 agcctgatta ccctccggtc gccgatccga tgctctgtga acctgatcag ccattcgtgg
     6601 ctcaccctgt cgaagaactg actgatgtcg gcatccagta cccagtttac gttagtgcgt
     6661 accagccctg tggccagtgc gtccagtgca tcgtgctggc ttcgcccggg tctgaacccg
     6721 tatgagaacc ccataaagtc gttttcatag actgcgttca ggattttcac cagcgcatac
     6781 tggacgatct tgtcctccag cgaggcgatg ccgagcgggc gttgttttcc atccgctttt
     6841 gggatgtagt gacgcctgcc gggctgcgcc ctgtagctgc cctgatgtag cctccggtgc
     6901 agatctgtta tgttgttctt catgtttccg gcgtagtcca tccacctgat gccatccact
     6961 ccggcggccg ctttcctgct cagggagagg aatgcggctt ccagtgcttc gactgtcagc
     7021 aggtggaaca atgctgtaaa ccgttctttc ttccgctgct tcgcagcttc ccgcacgcgt
     7081 gacagcctct gtgacatgct ttcccggctc tgtgtccggc gcatgtgtgg ctgttccgcg
     7141 ttccccttgg ccccgctcct tcgctccact gactccgctc ctttcgggtt gttcgcctgc
     7201 ttcgccgcta ctatgagcga gtccgacttc tcctctccgt acatcaccgg ctatgactcc
     7261 tcgtcttccc ggtgcgggcc atctccgaca ctggcagatg gtcagagggg agatctcccg
     7321 gttcccgcgt agagatcgta ttgacatgcc agggtctcag accccgccgg gtccatgtgg
     7381 cactcgcagt atcgcaccct atgatgttgc cttccgttaa cagtacaacg tcggcacccg
     7441 gtaatttaat atacatttcg tggctcaatg gctggcctgt caacacccct gtcaacgctt
     7501 cgccccatac ctcgcggtat gcaacgcatg actcggggac cttgtggatt gctggtcctt
     7561 caatggtcgg ggactttcac cccttgatct ctaaccggtc tcccggcgca cactgtacat
     7621 atacacagta taattattac cttactcctc ccgttttcag gtgtcaatat atgagattag
     7681 ttctcatctt caggcacaaa aaagcgcccg gagccacctg taagcagatc gcgtcttttc
     7741 ccgaaaattt ctcatggcaa caaattttat tatttaagac attcatcaca cttacatttc
     7801 agatggtata gacacatctg aaagctaact ggcatgagga gaggacctgc ggttaaactg
     7861 ttcgggaaac ctgagacgcc cggaggccac tatgacgcac ctgaaactga ctcccgctga
     7921 agaaaagcgc ctggcggacg cctttgtcgc tcaggcactg gccatgggcc tgaaagggaa
     7981 caaaacgctg ccaaaggcgg taaaacaaca ggtgcgggaa agggtgcttc gtgccattca
     8041 ggacgaacgt gaagccaggc gggcgcagga aatgcgtcgg gagaaatacg aggctacgga
     8101 aaccaccttc acctggcagc cacagcgccg ccggtaaggg gtatttacca taacgtcgca
     8161 ttgcccgcac cgctcagacg gtctgagggc gtttccggcg gctacgggat aaaacaggca
     8221 acaaaccacc cggaaaaccg ccggaaacgc gcccggtaaa ttgtaacccg ctgcatgcct
     8281 atgcacgcaa ctgcatggcg ggccgtttgg tgtaaaaacg atacgataag ccgtgtttat
     8341 gtaaatgaac gtggccgtaa agagtgacaa atatacatgt aacatttttt actgacaggt
     8401 catcaacccg atgcgtttaa taccctttat cctgctggca ttgtgcagcc ccttactcct
     8461 gacggcctgt aagcattcca ccacccagcc gccgaaaatt tatactctgg ataagtcatc
     8521 taaaatcagt agctacgctg cggccatcaa gaccgctgtt gaaatgcaga tgtttgaacc
     8581 agaggcctat gcgggaaaaa tgtgcaccgt gcggatatcc atacaacgtg atggcactct
     8641 gaacagcgcc attgctgaag gaggagatcc gaaactctgt aaagcagcaa tatcagccgt
     8701 tacccacgcc aaaattccgc cggcaccgga tgaaaatacc tggcaaatat tcaaaaacgc
     8761 ccctctggat ttcagaccat gaaagctttt actctgacag tgctggcggc catgttcctc
     8821 actccggtac atgcgatacc actaaaaccc gtaaccacac tgcagccaac ctacccggta
     8881 caggccgcag caaaacgcat cgaaggtcag gtgaccgcca cgtttgatat tctggacgat
     8941 ggacgagtgg acaacgtgtc gcttaccggc tgtcaccttt tttttcggga aacgctgacg
     9001 gcaatgaggc actggctata tcccgcaggg cacccggcaa tgcgacaggt tataaatttt
     9061 gaattcagtc tgaaaaatga cggcttacag gatccgcgac cagtcaccac ccaaaagctc
     9121 aatgatgatg gttgccgcca gtaacaggtc ctttaacccg caggctgcaa tagtcctgga
     9181 tgatgagtga gcctgtagca gccctgatta gtggaagcga agctgaaaaa tgggcagcaa
     9241 aagccgtatt tctggcaaat agcgccgcca cggattaaag tagtcatgtg taatacatat
     9301 gactacaaag gggcttttat gtcatcagca acatccatca ggctcgatga agaacttaag
     9361 gaccggctga aaacgctggc cgatgaccgg caccgctcag cgcacgcgct aatgctggag
     9421 gcaatcaccg aatacatcga ccgggaagag aagcgcagcc agtacctgcg ggacggacag
     9481 gccgcatggc agcactatca ggaaacgggt ctgcacctta ctgcggaaga agcggaagcc
     9541 tggataagta cctggggaac tgagaacgag caggatgctc cgccatgcca caggtaacga
     9601 tttccgcgct ggcacagcgc gatttacaac gccttcagga ctttctcaaa accaaaaatc
     9661 ggctggcagc cagaaaagga ggtgaggtga tcgtccgggc tatccagcaa ctgaaaacat
     9721 tgccagacat tggccgcccg gtgccgtttc tgccgctgga atataaggaa ctggtgatcg
     9781 ggtttggcga cagtggctat gtgatgctct accgccacga ccgggaaatg gaccagattg
     9841 tgatcgtcac cgtcaggcat cagaaagaat ccgggtatcc gggggcagac agcctctaat
     9901 aaccgatgac gggggcgcaa ttcattgcgt ggccgtcgcc ggtttgacaa gcgcagcgcg
     9961 tcagaaaacc acatcctgaa cggaaaaacg ggtaaaattc agcgatacat ttaccgtgag
    10021 aaattacaca cctttcacac ccgataaaat caggggtata cagtggcgaa aacattgctc
    10081 aacctttcac aggcagcaca agccgcgggc attaccagaa gaacgctata caatcatgta
    10141 aaacagggaa aggttaccgt gtcacgtgac gggaaaaaca accctgtcgt ggatgtgtca
    10201 gaactgatcc gtgtatacgg aaatgtgaat atccctgaga aacaaattcc cggcatttca
    10261 caccgtgaaa atacacaaaa aaatttccca caggaacagc ttctggcaat gcaaaaggaa
    10321 ctggccgatc tccggcaggc tgtcaccctg atgctggaag ataaaacatc ccgggaagaa
    10381 gaacgccgcc agcacgatga tgagcgccgg aaactccagg ccgaagtgga cagattgaca
    10441 acagaactga cacagaagaa aaaacgcttc tggtccggct ggtttagctg agaacttacc
    10501 gaagggaatg gagaacatga cgaagcagga aaaaactgcc ctcaatatgg ctaaattcat
    10561 taaggatcag tcgctgctgt tgctggaaaa gctgaacgag ctggacatgg ataccccggc
    10621 cgacatgtgc gaacggcttc acgaggatgc ggaaacactc tggcaggtga tgcgggataa
    10681 gctcggggaa gaataacagc accggccatg ccggtgctgg cgttattttt tgccgaacgg
    10741 ccaccaccgg cgggcgggtt tgtcctccgg cagttcaggc accggcacgt taaaatagcg
    10801 gctgttatat tcccgcatca gctccgcttt cagcttctcg ttcaccgcgc tttccccgtg
    10861 ccgctcgctg accacctcaa tcatcgcgtt aatctgctcc ggtgagcact tcccggcatc
    10921 aaccacctgc acaccgttct cgatggcgaa ccgctccacc tttcgataga ggtaatcaca
    10981 cagcttcatt cctgtgggtt tgtcccgttc ccattgcgga ttttcaccgg tgaaaaagta
    11041 cgtggctttt tcgtcgctgt tgagccgctc gtcatacatc ccgatggatt tcatcatcgc
    11101 cagatgctgg gcccggtcga caaaacagaa aacatacgcc ccggtctgcc aggcgagcac
    11161 caccatgtct tccagatgct cgctggttgg cataatgcag cgctcgccgt ctttgtcttc
    11221 cacgtcctca ataaatacca tgtacagcgc cataatccct cactccgttg tcataaattt
    11281 aaagcacgcg aacagcctct cccggtccag ctccaccgtt ttgcgggcct cgccggtctg
    11341 tggattcacg atttcatcca ccgccagaat cgcccgctgt acccggacaa gcgtcggccg
    11401 gtcagggcag atgtaccaga tttcatcgat gccgttcgcc cggcggttga acagataccc
    11461 ggacaccact tcctgatacc gcctgcggct cttgacggtg cgctctacct caaatgccac
    11521 cgttctgccg tccagctccg ccagcgcgtc cggcactttc atccctttca gggccatacg
    11581 gtgcaggtac cgccagccgg tggccccccg gccctccatg gagaggcgca gggactgcac
    11641 ggccagccgg tgcggcagct gcgccgccga cacccttgac ggctgaaacg gaataacgtc
    11701 cgtcagcggc tcgtcctcgt cgaacgagaa cgccaccccg tgcggcgtca gcccccacac
    11761 gctgacctga cggccgtcaa ccgggtattt cgcccggacc agcagccctt tcgccaccag
    11821 actgtccagg aagcggtaaa ccggctgtac cgccgcaatc cccagccgtc gcgcaaccag
    11881 ctccgccgtg gaacacagct cttcccgcag ccagcgcagg gtcagccgtg ttttctccgc
    11941 gttccgcgcc tgccgcttcg cgtggctgac
//
