LOCUS       NZ_CP039300            27728 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli strain PigCaeca_1 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP039300
VERSION     NZ_CP039300.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11268909
            Assembly: GCF_004801575.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27728)
  AUTHORS   REHAB consortium,T.
  TITLE     The impact of sequencing depth on the inferred taxonomic composition
            and AMR gene content of metagenomic samples
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27728)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2019) Modernising Medical Microbiology Consortium,
            University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU,
            United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30x
            Sequencing Technology :: Hybrid assembly: Illumina HiSeq 4000 and
            PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004801575.1-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 07:24:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,728
            Genes (coding)                    :: 4,527
            CDSs (with protein)               :: 4,527
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 201
            CDSs (without protein)            :: 201
            Pseudo Genes (ambiguous residues) :: 0 of 201
            Pseudo Genes (frameshifted)       :: 56 of 201
            Pseudo Genes (incomplete)         :: 140 of 201
            Pseudo Genes (internal stop)      :: 40 of 201
            Pseudo Genes (multiple problems)  :: 28 of 201
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP039300.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..27728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PigCaeca_1"
                     /isolation_source="feces"
                     /host="pig"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="United Kingdom: Yorkshire and the Humber"
                     /lat_lon="53.91 N 1.03 W"
                     /collection_date="2015"
     gene            complement(join(27685..27728,1..106))
                     /locus_tag="E7Z58_RS24480"
     CDS             complement(join(27685..27728,1..106))
                     /locus_tag="E7Z58_RS24480"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001816708.1"
                     /translation="MNNDILALAEMIKKAIDSHTSFKLPPLSGSEFKAVMEALREQNAG
                     FQAV"
                     /name="hypothetical protein"
     gene            complement(202..423)
                     /locus_tag="E7Z58_RS24055"
                     /old_locus_tag="E7Z58_24065"
     CDS             complement(202..423)
                     /locus_tag="E7Z58_RS24055"
                     /old_locus_tag="E7Z58_24065"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000798354.1"
                     /translation="MKNSNFEEANEINNAINEMISERQSIDAGRNDFLQETWLLVRLGH
                     QYKEAGKDWYRWAKATAIRLWESGHIVK"
                     /name="hypothetical protein"
     gene            complement(788..2125)
                     /locus_tag="E7Z58_RS24060"
                     /old_locus_tag="E7Z58_24070"
     CDS             complement(788..2125)
                     /locus_tag="E7Z58_RS24060"
                     /old_locus_tag="E7Z58_24070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_072795998.1"
                     /translation="MATSQLALFNPADEAETYHNSGRHGFFSVLAQWPTGKRQSSYPLS
                     EMATVLSMVDTSRDTWITQAEFMRPNRRVVNLLRLGLLFVDIDTYKMSWAQGRTPEALA
                     AAINYFCQQEGIPEPSFMVYSGRGIQAKWLLEGTLPRAALPRWNACQKHLVSKLSSLGA
                     DPAAKDASRVLRLVNTVNTKSGNICRVVSVLERDGAPIRYNFEYLAEMLLPVARWDIEK
                     QRQTKAEQKLQLLPGGRQSNLRGFSGRQLAWDRLEDLRKLDELRGGLAEGERMRHLFWR
                     INFLLLSGATNSTQMYHEAAALATEISPAWSYRSQELMTLYSKAKQYEAGEKVSFGGKE
                     FSPLYTPKNDTLINLFQITDEEQRQLKTIISKNEALERRRARDRKRDEERRRAAGAVER
                     DVYEANSLTKQKPWEVLGMSRAKWYRLGKPSGSETTPSPITNGVAP"
                     /name="replication protein"
     gene            complement(2308..2622)
                     /locus_tag="E7Z58_RS24065"
                     /old_locus_tag="E7Z58_24075"
     CDS             complement(2308..2622)
                     /locus_tag="E7Z58_RS24065"
                     /old_locus_tag="E7Z58_24075"
                     /function="Plasmid maintenance system antidote protein"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077082.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_015505594.1"
                     /translation="MNQTQVLRPATPGDVLLSQFLEPNRISQTALATYTGWTRKHVNQL
                     CHGKVAITIDSALILAKVFGNNPDFWLDLQKKVDLWDALHSEDRRSRIDKARPFQLLAA
                     "
                     /name="HigA family addiction module antitoxin"
     gene            complement(2943..3314)
                     /locus_tag="E7Z58_RS24070"
                     /old_locus_tag="E7Z58_24080"
     CDS             complement(2943..3314)
                     /locus_tag="E7Z58_RS24070"
                     /old_locus_tag="E7Z58_24080"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_057517901.1"
                     /translation="MRTFKGLSNQNRSPTSNVVPIRPGGGVASNDDNSGAAKAKWIAGA
                     TAKGLGSVLRSLTFLIMLWLRLPVRLLLVATSAIAFIGVVLCLAIFSDRSMVPGFIIIG
                     LASTALAWLYDLVLAKLTP"
                     /name="hypothetical protein"
     gene            3499..3807
                     /locus_tag="E7Z58_RS24075"
                     /old_locus_tag="E7Z58_24085"
     CDS             3499..3807
                     /locus_tag="E7Z58_RS24075"
                     /old_locus_tag="E7Z58_24085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_001098859.1"
                     /translation="MNTVKIIAVALGLTLISAMPAQAKDPCKAVLCLAGMLTGNDGGSE
                     CKSAVNDYFSIVKFKKGKFKKSATKEARGAFLQQCTSEDEGWINKINSAYGTVRKGF"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            3841..4587
                     /locus_tag="E7Z58_RS24080"
                     /old_locus_tag="E7Z58_24090"
     CDS             3841..4587
                     /locus_tag="E7Z58_RS24080"
                     /old_locus_tag="E7Z58_24090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850363.1"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="WP_000418737.1"
                     /translation="MELFSILATCAALTFGTGSPAEQAYPVSEQECKSAAAMYREQLQK
                     VQLTDQDRDAIARVAIAEAANQGDSGLAGVVYTIINRRLSGQFGQTVTDVVNSPKQFEP
                     VTKVGGDWKKLPSVTVSQYTQVQTIINLALEGHLPDITNGGLFFQNPAIVADREKAGKV
                     SKGLTNFGGSAPSAIIKDHAFYASINKGGPSNAPPVQLTASAVPEPKKWDVYGTAKFQK
                     NSNAEWNVFSKNTPSKSVYQNEGVQP"
                     /name="cell wall hydrolase"
     gene            4584..6566
                     /gene="topA"
                     /locus_tag="E7Z58_RS24085"
                     /old_locus_tag="E7Z58_24095"
     CDS             4584..6566
                     /gene="topA"
                     /locus_tag="E7Z58_RS24085"
                     /old_locus_tag="E7Z58_24095"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772478.1"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003917 - DNA topoisomerase type I (single strand cut,
                     ATP-independent) activity [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006265 - DNA topological change [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="WP_097290435.1"
                     /translation="MKLMIVESPGKVKKIREILGDGWKVAASVGHVRDLPEKDIGVAAP
                     DFKPTYVATERGKEVLAKLKQDVQHSDAVFLATDLDREGEAIAWHLKQALRLENPQRVT
                     FAEITPRAIKAALASPRAINIPMVASQEGRRVLDRLVGYMVSPRLSEMSGRQGLSAGRV
                     QSPAVRIILERERAIQNFKPTAHFGVILNFAGDWFAEWDVKPFLAEGSEYFMDEGFAKK
                     IAAIREVDVISYEEKNASKAPPAPFITSTLQQAASNKLKMKPKATMTTAQKLYEQGHIT
                     YMRTDNPNLSDDALVEIYQLCKAKGLPMASKPRTWKAKGDAQEAHEAIRPSHFDVEEAG
                     STPEEKALYRLIWDRAVACQLESARYAVRVARFLASDNSDGKPVEFEAEGRTLTYAGWT
                     TLFKDDTEADDGKKEEKPKNPIPSLQRGQHLTAESGKVLSKTTRAPSRFTEASLIAELE
                     RLGIGRPSTYAAIMENIMRRGYVKSDSANKYLLPTETGALVIDALVKGGFSFIEYDFTR
                     AMEGELDKVAHGQTSYKNVVGSVYGRLESELSHLKIDVAPQYPCPNCGKALFRKKGATG
                     FFWGCSGYPDCSTTLPDTAGKPGKPKPKPEASGFKCPDCGKPLIRRKKPATGGKKGYDF
                     FGCSGYPGCSSKFDAVNGKPKFEKK"
                     /name="topA"
     gene            6704..6958
                     /locus_tag="E7Z58_RS24090"
                     /old_locus_tag="E7Z58_24100"
     CDS             6704..6958
                     /locus_tag="E7Z58_RS24090"
                     /old_locus_tag="E7Z58_24100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010895201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072141155.1"
                     /translation="MNNKTITEPELIDNLVNQSVKKMMLVQGEDGKFSIRVQLSWKGGD
                     LLLETQRKTPRVWASLDRLVKHINSKYGKVPIIELQLME"
                     /name="hypothetical protein"
     gene            6966..7274
                     /locus_tag="E7Z58_RS24095"
                     /old_locus_tag="E7Z58_24105"
     CDS             6966..7274
                     /locus_tag="E7Z58_RS24095"
                     /old_locus_tag="E7Z58_24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000024420.1"
                     /translation="MANVKTFTRQIASTTWLMLGLMMLPGFAHAAGGDPINQGLQWLID
                     LLTSGIARSCAILAIAVLGYLAWAGRLQARNAGFAIGGIVLVFGAASIADAIIAVVQ"
                     /name="TrbC/VirB2 family protein"
     gene            7285..7602
                     /locus_tag="E7Z58_RS24990"
                     /old_locus_tag="E7Z58_24110"
     CDS             7285..7602
                     /locus_tag="E7Z58_RS24990"
                     /old_locus_tag="E7Z58_24110"
                     /inference="COORDINATES: protein motif:HMM:NF016957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="WP_000146200.1"
                     /translation="MASGKDVLFAGATRPSLVWGVTYEAIIFCIATTGVIFLAANSIWL
                     LPLYVPLHGACYLICLKDPRFFRLISLWFATKCRSIGWRYWGAATASPLVSTRKRRKMP
                     E"
                     /name="VirB3 family type IV secretion system protein"
     gene            7599..10049
                     /locus_tag="E7Z58_RS24105"
                     /old_locus_tag="E7Z58_24115"
     CDS             7599..10049
                     /locus_tag="E7Z58_RS24105"
                     /old_locus_tag="E7Z58_24115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_000063657.1"
                     /translation="MSNATATKSRQWVNENSVAEFIPYSVHLDENTIKTTSGYYLQVIK
                     IDGRAHESADPEDVINWKEQLNGLLKNIASPNISLWTNIIRREENTFPGGEFEEGSFAE
                     QLNNKYKNHVGKNNMMVNELYLTVLYRPSGIPGVNLLQRFEKNVESIKRIQAEAIEGLH
                     EVVNTVTSSLKHYGARVLGCYDRKGIKNSEVLEFFSYLINLEWIPRALPRKVLSEALPF
                     DRPFFGEDAFEVRGVTESTVGAVLSIGEYPEGTEAGLMNAFLSAPFPLILSQSFNFLSK
                     PVATELLGRTQRRMKNAGDFSKTQMEEIDDALDDLTSGRLVFGEHHLALTILAKDARSL
                     KKNLSDAKAELVDCNMIVSREDWAIESAFWSMLPGNFKYRARPAPISSRNFAGFSSFHN
                     YPTGRRTGNQWGPSVTLLKTTSGAPYYFNFHSELSAEKARKLAQLEAEKGAGSIQETKE
                     EQKALGNTLIIGPSGSGKTVTQGFLMAQSKKFNPTQIIFDKDRGLEIYVRAEGGVYLPF
                     KNGARTGCNPFQMEPCEDTYQLLEQIVKKCAGGSFTTVQDTDISNTVRGVMRLPKHLRR
                     ISACLDFMDPTDPEGVGARLAKWCGNGTLAWVFDNETDDIDFSNNTMFGFDVTDFLDNA
                     EIRTPLIMYLFHRISQIIDGRRMMIFMDEFWKLLLDEYFEDFAQNGLKTIRKLNGLMVF
                     GTQSAKDVLKSAIGYSIIEQCATMVFMPNPKADWDDYVKGFKLTEREYQLIKTDMAPGS
                     HQFLIKHGHNSVIAKLDLAGFSDELAVISGTADNVELLERIMQQVGDDPKVWLPEFHKQ
                     RVAA"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            10071..10715
                     /locus_tag="E7Z58_RS24110"
                     /old_locus_tag="E7Z58_24120"
     CDS             10071..10715
                     /locus_tag="E7Z58_RS24110"
                     /old_locus_tag="E7Z58_24120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000768506.1"
                     /translation="MKLKKLALVLAIAGSLNAVSYAGVPVSVVADPQAWTAHLEDMAKY
                     VEQIKQLENQFKQQVKQYESMTSARGLANVINSQYDTDALSGINTDSILRDSGINSASD
                     FKLPEDVAELYDTAAKNAATYAGQASRSLEQAQSRFTELSGLVRRVNTATDPKEVMDLN
                     ARIGAEQAFLQNEVGKLQVLQQTAQANDALYQQKVKQMAVQSSGSLRNVNW"
                     /name="type IV secretion system protein"
     gene            10724..10972
                     /locus_tag="E7Z58_RS24115"
                     /old_locus_tag="E7Z58_24125"
     CDS             10724..10972
                     /locus_tag="E7Z58_RS24115"
                     /old_locus_tag="E7Z58_24125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000466217.1"
                     /translation="MFFSCARKISAVCLVSLALTGCFDKEETHSVEWFSNHNAERAETV
                     KRCSDNPGELGNTPNCKNAIAAEQKASSGSLRHINNW"
                     /name="EexN family lipoprotein"
     gene            10988..12001
                     /locus_tag="E7Z58_RS24120"
                     /old_locus_tag="E7Z58_24130"
     CDS             10988..12001
                     /locus_tag="E7Z58_RS24120"
                     /old_locus_tag="E7Z58_24130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000536623.1"
                     /translation="MGTGTNLFVRIFANVDSALSTYITDTVGNVIGYASPLFTSMMIVW
                     IAMWGILMMFAKVDEPVRDGVFRIIRIGGIVALGLTVGTYMDVVVNVLQKGPEHISAVV
                     SGTTGTTADTLDALFTKIFNVAKAAWDKGGVMNGNFGLYLIALIVLVVGCGLTLFVAFL
                     VLLSKFMITILLGIGPLFIIALLFNTTQRFFEAWLAMVCNFGILLILSASVGSLMISLG
                     DTYISKMAPTESAAANMANLGDAAMLCLVFALCILVVRQVPSIASALGGGVAMATQGAF
                     GSAMNALRPTAMKRGMQNVNREVKTTQRAMTSPVRGGQAAYTSYQKRFGGNSVAQG"
                     /name="type IV secretion system protein"
     gene            12143..12361
                     /locus_tag="E7Z58_RS24125"
                     /old_locus_tag="E7Z58_24135"
     CDS             12143..12361
                     /locus_tag="E7Z58_RS24125"
                     /old_locus_tag="E7Z58_24135"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000748928.1"
                     /translation="MKKSLAVIALTFTLGACTLTPPPPAEAVDDGRGMVPINPAMVTQE
                     QINAVSSKYKDVNKKQVQQLKEFAHGQ"
                     /name="hypothetical protein"
     gene            12351..13109
                     /locus_tag="E7Z58_RS24130"
                     /old_locus_tag="E7Z58_24140"
     CDS             12351..13109
                     /locus_tag="E7Z58_RS24130"
                     /old_locus_tag="E7Z58_24140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284509.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB8/TrbF family protein"
                     /protein_id="WP_057517899.1"
                     /translation="MANKKAAPAKEKKVTDKDYFDEAKSWDESEIVREKKSARRAWSAF
                     WAMTGVVIVQAIAISTMMPLKTIENSIVRVNDTTGETEVISNLKNMDETTEQVMSRYWL
                     AKYLRHREGYHWNTREDDRLQVGMLSDRAIQQQYADYTNPKVNPYAPIKIYGETTEVDI
                     KVNPAITYLNGKGGVKPEKGEKDQFGETVYTALVRYTATVKKDGEMPVTTHWAATVSFV
                     YRKEPIKVDDRLINPVGFQVISYRKDQEGG"
                     /name="VirB8/TrbF family protein"
     gene            13112..13933
                     /gene="virB9"
                     /locus_tag="E7Z58_RS24135"
                     /old_locus_tag="E7Z58_24145"
     CDS             13112..13933
                     /gene="virB9"
                     /locus_tag="E7Z58_RS24135"
                     /old_locus_tag="E7Z58_24145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_000950096.1"
                     /translation="MLSLNKTSLSVLLVLGIFSSTAFALEVPRGGAYDKRVKFISYNHE
                     QVVKLIGHYGYSTDIQFAPGENVTNIAMGDPDAWEVAPVENHIFIKPTGEQATTNMTVL
                     TDRRVYTFDLSATGKKTGDMYFQVNFRYPQDEAARRVAEEDARRLKERMNEKAPNAWTN
                     WNYWVFGSREVSPWRAYDDGRFTYFKFSGNTSIPAIYIKHADGSETLLNPTMDSDHPDT
                     MIVEKVAPRFVLRNGKYIANVYNESFNPHGLINTSGTTSPGVKRVIKGGAQ"
                     /name="virB9"
     gene            13933..15123
                     /gene="virB10"
                     /locus_tag="E7Z58_RS24140"
                     /old_locus_tag="E7Z58_24150"
     CDS             13933..15123
                     /gene="virB10"
                     /locus_tag="E7Z58_RS24140"
                     /old_locus_tag="E7Z58_24150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB10"
                     /protein_id="WP_001040129.1"
                     /translation="MAKDRDDLDTEIEQEDADRGMPSISKNRKTGKASGIGQKIVAVII
                     GIIVILALIAVNGGFKGSEEDKPPKLTGNTSVANRLGPPPSLPPPPPAPPNTETPKEDR
                     MLNTGQIAPPPPSSASGTGGNGKHEQTPDERKRASSILAFGSISKPQQPGQKAGNGQPV
                     QMAGEVQEGNSSLSETDRADSLSNRLKGTEVSGSRASLLVDRSYFITQGTFLNCALETA
                     ISSDVPGMTACRLTDDVYSTDKKVLLLEKGSKIVGQYQGGIKRGQARIFVLWTRIETPK
                     GVLVKLDSPGTDALGRSGLDGFVDTHFWERFGSAIMLTTVDGLITYGANSANNGSNGNQ
                     YNFGDTAQTARDSASIALENSINIPPTLTKNQGDNISIFVARDLDFRGVYDLKAAY"
                     /name="virB10"
     gene            15101..16138
                     /gene="virB11"
                     /locus_tag="E7Z58_RS24145"
                     /old_locus_tag="E7Z58_24155"
     CDS             15101..16138
                     /gene="virB11"
                     /locus_tag="E7Z58_RS24145"
                     /old_locus_tag="E7Z58_24155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703991.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000172959.1"
                     /translation="MTLKQPTNAKGSSLLEYLRVLQPFLNEDGVTEVVVNKPGEVITEG
                     RKGWQFHNVPKLDFAACADISKLTATYSGQSFDERKPIVSATLPYGERIQIVRPPATLS
                     DRYSLTIRKPSDVIRTLDDYERDGAFDHIVTDAVGLRDFERELIELKNARRFKEFLTLA
                     IKHRQNIIVSGATGSGKTTFMRSLLQLVPDDERLITIENVDELKLYQTHKNVVPLFYSA
                     GGQGIADVTQQHLLESSLRMKPDRVFVAELIRGDEAFYYLRNVNSGHPGSITSMHGTNP
                     RMAIEQLVLFIKESQSGGTMSREDIKQLLFSCIDIIVQFQNHQGRRYISEIYYEPEFKQ
                     NILVA"
                     /name="virB11"
     gene            16101..18029
                     /locus_tag="E7Z58_RS24150"
                     /old_locus_tag="E7Z58_24160"
     CDS             16101..18029
                     /locus_tag="E7Z58_RS24150"
                     /old_locus_tag="E7Z58_24160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703990.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_000083589.1"
                     /translation="MSQSSNKIFWWLKILLLISLPLIFLAVAAWIAGAIFMAGNGYEAK
                     DATPLTLYQYWYYYGDIPKTKLWLMGSLVGGLIITALPCLLPLLPKKTPLHGNARWANS
                     REIREAGLFSDDGIIVGVKKGFMGLFMQYLVFSGSQHVLMAAPTRSGKGVSIVVPNLLS
                     WRDSVVVLDIKQENWDITSGFRSRHGQDCFLLNLAPRDYRSHRWNPLFYISDDPNFRIN
                     DIQKIGQMLFPKIENEAPIWQSSARSLWLGMVLYLIETEELPVTMGEALRQLTMGDERL
                     AEIVEARQESETPLSDECYLALKEYLDTPDKTRGSVRKGFTSALELFYNPVIDAVTSGN
                     DFDLRELRKRRMSIYVGITPDDLERLAPLINLFFQQVIDLNTRELPEQNPDLKYQVLML
                     MDEFTAMGKVNILSKGISYVAGYGLRMLPIIQSPAQLRETYGHDAAETFIDNHALQIVF
                     APKNLKIAKEVSESLGTKTVKARSRSRSTIGRASGSENTSETGRALLMPQELKQMGKSK
                     EIILLEDCPPIQCSKITWYKDPTFEARGNGRDGVKFPSPEVTIIDPANRPKGEVSFMSN
                     RVEQAEETKTEVVERAVTVADIENIDKLNLDDFSCDFSKVEIPKGDITDDAMDDIVNQF
                     FSGLEAA"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     gene            18044..20632
                     /locus_tag="E7Z58_RS24155"
                     /old_locus_tag="E7Z58_24165"
     CDS             18044..20632
                     /locus_tag="E7Z58_RS24155"
                     /old_locus_tag="E7Z58_24165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="WP_079904175.1"
                     /translation="MNEPNLATKNTVLIVSFEERNEAKKAAGKLENGENALGFDEEAKH
                     WFAKPGADLSKLSKWLPDQHKGNPVQDNNPAAEFGATLAEAGFELDGLPVMDGKKHRVR
                     TADDKKGEKTGVYAGYLDGVPAGWYQDHRIHDQPVKWVSTGQQVDAEAQAHLRAIAAQN
                     RIDRENALQRQYAHNARRSGQVYNAMPVADGSQEYLVNKGVKAFPGVKADKKNRVVIPL
                     VNEEGEVRTLQRISANGFKSLKKNGQKTGNFFVVGGELKNGEPVLYAEGYSTAASISEA
                     TNRPVVMTVDAGNLPKVADKLKDKYPDSLHVVLGDDDRKNAVNKGMAKAEEAATIANGV
                     FTVPAFTAEEKERGLTDFNDLHQSRGLDAVRDQVEGIITEHTHKAETMADKEHEAINTG
                     PVITENFEREMDAALNVNHSSIDLEPYQSEEDEYDVENSIEPHIEQEKAQTADDDVRAW
                     LSSSRVSTADMLAEDDEREGRKREEFKQVEPDAEEPVFKRQSQDQDYFEDSSYKPVVPD
                     RVAKSYIEVEGKYYFQNRPDSLAFVDKGAKLQTKLSNSQVAGSMIDIAEARGWTEIQVK
                     GTEDFRREAWLQATARGLTAHGYKPKEEDLARLRKVAGERNINEVEAREVADNVKTSPS
                     APSGTTSQNQSEAPTSKTGTTESSSTAQGKNEGAKVEAEEKVNKLAGKLVEHGAAPYEH
                     KKDNKDNYYVTLENADGKQSTTWGVDLQRAMAESEAQPGDHVELENLGRKAVTVEKDVK
                     DETGKVVKKETINTFRNSWQVKADAIRDQERPAREIVKEHPDLVNEITAVKLAEKVSQS
                     MNADDKERFMSRVRDKLADKVASGEKGPELKIKEVQTADNPRVKDTELER"
                     /name="LPD7 domain-containing protein"
     gene            20696..20848
                     /locus_tag="E7Z58_RS24160"
                     /old_locus_tag="E7Z58_24170"
     CDS             20696..20848
                     /locus_tag="E7Z58_RS24160"
                     /old_locus_tag="E7Z58_24170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006706362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_036983224.1"
                     /translation="MNNKILDLNIPGREIEMSRQEAEELGAFEESALSETDAREAIDIE
                     DEQEG"
                     /name="hypothetical protein"
     gene            20852..>21760
                     /locus_tag="E7Z58_RS24165"
                     /old_locus_tag="E7Z58_24175"
                     /pseudo=""
     CDS             20852..>21760
                     /locus_tag="E7Z58_RS24165"
                     /old_locus_tag="E7Z58_24175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122509.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /name="zincin-like metallopeptidase domain-containing
                     protein"
     gene            complement(22072..23064)
                     /locus_tag="E7Z58_RS24170"
                     /old_locus_tag="E7Z58_24180"
     CDS             complement(22072..23064)
                     /locus_tag="E7Z58_RS24170"
                     /old_locus_tag="E7Z58_24180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006706478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000257690.1"
                     /translation="MAVESLSKLLEGKVKTKSNKTTDLKSKASRIANRASEVMVKVSGN
                     AKGPAHVKAHLDYISRNGKLELENERGEILKGKDDVKAVHKEWTQDQGKRRANTRDTTN
                     VVLSMPKGTEAKAVKESARAFAKKQFGENYQYVFALHEDVDHPHVHISIKTLGFDGRRL
                     HVKKGDPQIWREGFAKELRARGVEAEATPRATRGVVKKGIDQAILHIRKRGLTPEVDKA
                     KIKEIVEEFKDTRAGKPAKAKPWEKKIQTRQQEVRKGWLSAAQELNQSSEPGDKKLAAD
                     VVNFVNTMPPMATERHEMAKAVSQKLASQQPGKKQNSTGRDRDNDQEER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(23067..23582)
                     /gene="mobC"
                     /locus_tag="E7Z58_RS24175"
                     /old_locus_tag="E7Z58_24185"
     CDS             complement(23067..23582)
                     /gene="mobC"
                     /locus_tag="E7Z58_RS24175"
                     /old_locus_tag="E7Z58_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013333055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_072796054.1"
                     /translation="MPRIVKRITPELKEKWKTFCHERGVSESDMLGLLLEKATAGIVPN
                     EAKGLEEPRSDKVTIRLSPKDIHSMTERAKAEGFPSRTTWLTNLVLATLSKEPVLTDAE
                     VNALRGSNRELAAIGRNLNQIARALNVEFRDSDKLNKELIDRLLEQIDGHRDKVSNLID
                     RNMNRWGG"
                     /name="mobC"
     gene            complement(24299..24538)
                     /locus_tag="E7Z58_RS24185"
                     /old_locus_tag="E7Z58_24195"
     CDS             complement(24299..24538)
                     /locus_tag="E7Z58_RS24185"
                     /old_locus_tag="E7Z58_24195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000797250.1"
                     /translation="MKNRIVLISGVIAALVLILFLAYPYLKNSQSFLAVVAICVTFAVL
                     ALAIPANLFSWLRGKKGKPGFIKSTFIGILRRGK"
                     /name="hypothetical protein"
     gene            complement(24550..25674)
                     /locus_tag="E7Z58_RS24190"
                     /old_locus_tag="E7Z58_24200"
     CDS             complement(24550..25674)
                     /locus_tag="E7Z58_RS24190"
                     /old_locus_tag="E7Z58_24200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703985.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0007059 - chromosome segregation [Evidence
                     IEA]; GO:0000910 - cytokinesis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="WP_072796058.1"
                     /translation="MSAKKGLDLSGLDNFSALMGGDPAPTQSAASTDVPEGFAGNLIRV
                     PLKKIHKDADNARKTIDPQELRELADSMKSINPATGKPRGNKNPVSLKPHPDIPGEYIL
                     NAGERRCAAAELAELPDVLAFIDEDADEFDNAVDNIQRVALSPIETAAFIQRRIEKGDK
                     KSAIAARLGKPASFVSDHIIFFDMADCIRDMYDSGRVVSMQAMTLLHRAYKKHAEEVEA
                     FCSEAQDELTTADVRAFCDSLKEPESKAVNTSGDNEEGQELPSDANAWIGGGADSDELE
                     KGEEQQPLVIDDEPISGGSSSDLEDEASKIIAADEASDKIKKAIVQIKYDERPARLLTN
                     RRASYGFGWIKYDDDGSEDEIDLKDVSVVAIVEG"
                     /name="ParB/RepB/Spo0J family partition protein"
     gene            complement(25671..26423)
                     /locus_tag="E7Z58_RS24195"
                     /old_locus_tag="E7Z58_24205"
     CDS             complement(25671..26423)
                     /locus_tag="E7Z58_RS24195"
                     /old_locus_tag="E7Z58_24205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850378.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_072796060.1"
                     /translation="MNIITVTNQKGGVGKSTIAVHLAMALQERGDRVLFVELDPQGNAS
                     KTLEKAGGVAALQASQLFEEQQLTITPNEGITLINADAKMADIERAPLTVMSTFKDHLA
                     ALASQFDHCVIDTPPTLGLRMSAALIVANHVLSPIELEEYSIDGITKMLQTIFGVKLKW
                     NPDLNFLGMLPNRFNSRSEDQKKTLMNLVENYAHLLIRARIGIRSSIPEALSEGIPVWQ
                     LKKTSAREAGKEFQEAFKIIFEKMGVAK"
                     /name="ParA family protein"
     gene            complement(26380..26742)
                     /locus_tag="E7Z58_RS24200"
                     /old_locus_tag="E7Z58_24210"
     CDS             complement(26380..26742)
                     /locus_tag="E7Z58_RS24200"
                     /old_locus_tag="E7Z58_24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrfB-related DNA-binding protein"
                     /protein_id="WP_000746879.1"
                     /translation="MKKRLTQAEFEHAISNLSRALKPANVEIVKAILVDGRKQNDMVIE
                     TGLSRTAIAAMTKKVREAHKLHGKPPAGWERIELCIPSSMVPMLRAMEDEARKQANAKG
                     EMNEYHNSDESEGRGR"
                     /name="TrfB-related DNA-binding protein"
     gene            complement(26832..27341)
                     /locus_tag="E7Z58_RS24205"
                     /old_locus_tag="E7Z58_24215"
     CDS             complement(26832..27341)
                     /locus_tag="E7Z58_RS24205"
                     /old_locus_tag="E7Z58_24215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="WP_021265630.1"
                     /translation="MLKRWNIIFLFLFLPLQYAIADIHGEIIRIIDGDTVDVLVNKTPI
                     RVRLVDIDAPESKQPFGQKSRQFLADMIFRQQVTIVEKGKDRYNRVLGTVFKEDNTNVN
                     ESQVSNGMAWAYRYREQATNPAMLDLEQDARQKRLGLWSDPQSSIVEPWKWRRVNNKFR
                     AHETAK"
                     /name="thermonuclease family protein"
     gene            complement(27341..27643)
                     /locus_tag="E7Z58_RS24210"
                     /old_locus_tag="E7Z58_24220"
     CDS             complement(27341..27643)
                     /locus_tag="E7Z58_RS24210"
                     /old_locus_tag="E7Z58_24220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006954203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001066964.1"
                     /translation="MNNVIRTETLLPFSDGWTPPTGEEIKAVLSLVEERSGNKFTGVDA
                     SKYIGLPSETGRGKGSRTFRRWCKEGGIPYAAWALLCYKAGFGVIWESDEQRGEN"
                     /name="hypothetical protein"
     Typing          complement(807..2125)
                     /name="rep_cluster_312"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.697"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(22076..23064)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.727"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7286..7599
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.68e-63"
     Typing          7600..10046
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.755"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          10989..11998
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.11"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          12391..13106
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="96.234"
                     /coverage="95.0"
                     /evalue="5.130000000000001e-143"
     Typing          13113..13930
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.267"
                     /coverage="100.0"
                     /evalue="2.33e-172"
     Typing          16102..18026
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.377"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcaccgcttt aaactcgctc ccggataggg gaggaagctt aaaggaggtg tggctatcta
       61 ttgccttttt aatcatctcg gccaatgcta aaatatcatt gttcatggtt actctctcgc
      121 gggtttaacg tgaacttccc tgtatggttg cagccataac agggaacctt tcaaaaggcc
      181 cgcgtaagcg ggccacctga atcatttaac gatgtggcca ctctcccaaa gacggatagc
      241 cgtagcttta gcccagcgat accagtcttt accggcctct ttatactgat gccctaagcg
      301 aacaagaagc caagtttctt gtaggaagtc gttccgacct gcgtcgatag attgacgttc
      361 actaatcatt tcattgatgg cgttgttaat ctcgttagcc tcttcaaaat tgctgttttt
      421 catcggtatt ctccggtatt gaccgtagca acttgcccgg ccttcatttg taagggtagg
      481 ccaaaatgac ctacccgtca atcaaaatag tatactttga taatgcgata accaaaaaaa
      541 accccgctaa tagcgaggtc aattttatat gggaaactta gcccataact atcttgatac
      601 tggcaccaca ggccgccgcg tatcgttcaa gtgttgtgac ccagtctctt tattgatcct
      661 gaaagatcgg aaaaggatca aggcgtaaca gggaagaggc tacgccatta gtaatagggg
      721 agggagttgt ctcactgcct gatggtttgc ccaagttgct cttcatctgt aatttgaaac
      781 aggttgatta gggggctacg ccattagtaa taggggaggg agttgtctca ctgcctgatg
      841 gtttgcccaa tctgtaccat ttagcgcggc tcattcctaa cacttcccac ggtttctgtt
      901 tagtcagtga attagcttcg taaacatcac gctcaacggc tccagcagct ctgcggcgtt
      961 cctcgtcacg ctttctgtct cgtgcgcgtc tacgctctaa cgcttcattt ttagaaatga
     1021 tagtttttag ctggcgttgc tcttcatctg taatttgaaa caggttgatt agagtgtcat
     1081 tttttggcgt gtagagtggt gaaaattcct tgcccccgaa agataccttc tcaccggctt
     1141 catactgctt tgctttgctg tacagcgtca ttaactcttg tgaacggtaa gaccatgcag
     1201 gggagatctc cgtggccagt gcggccgctt cgtgatacat ctgcgttgaa tttgtcgccc
     1261 cggacaacag caaaaaatta atacgccaaa ataaatgacg catcctttcc ccttccgcta
     1321 acccgccgcg cagctcgtcc agcttgcgca aatcttcaag cctatcccat gcaagctgac
     1381 gaccgctaaa acctctgagg ttgctttgtc ggcctccggg cagcaattga agtttctgtt
     1441 ctgcttttgt ctggcgttgc ttttcaatat cccagcgagc caccggcaat aacatttcag
     1501 caaggtactc gaagttataa cggattggtg cgccatcacg ttctagaacg cttactacac
     1561 ggcaaatatt gccgctctta gtgttaaccg tattaaccaa tcgtaaaacc cggctggcgt
     1621 ctttagctgc gggatcagcc cctagcgagg aaagtttaga tactaaatgc ttttggcaag
     1681 cattccagcg cggtagcgcg gcacgtggca aggttccttc aagtagccat ttagcctgaa
     1741 taccacgccc ggaatagacc ataaaggagg gttctggtat gccttcttgt tgacagaaat
     1801 agttgattgc agcggccaaa gcttcaggtg ttctaccctg cgcccaactc atcttgtagg
     1861 tatctatatc gacaaaaagc aaccccaaac gtaagaggtt tactacacgc cggttaggac
     1921 gcataaattc agcttgggtt atccatgtat cgcgtgaggt atcgaccatg ctaagaacgg
     1981 tggccatctc acttaatggg taagaagatt ggcgtttacc cgttggccac tgagccagta
     2041 cagaaaagaa accatgccgc ccggaattgt gataagtctc cgcttcgtcg gctggattaa
     2101 aaagtgccag ttgtgatgta gccacgtcta ccccttagac ttgccaagag gcacaacacc
     2161 gctataatag cggtgttgta acccttagcc tatgtcaggc aataatagtt gtgttgcagc
     2221 ctgccagctt tagaacacaa ctttgaattt taagccccgt ttgttgacct tcccgccaag
     2281 tcggatcagc atcggggttt taccttttta ggccgccagt aattgaaaag ggcgcgcctt
     2341 atctattctg ctgcggcgat cttctgaatg cagtgcatcc cataaatcga ctttcttctg
     2401 caaatccagc caaaaatcag gattgttacc aaagactttt gcaaggatga gcgcggaatc
     2461 tattgttatg gccacttttc catgacagag ctgattaacg tgctttctcg tccatccggt
     2521 ataggtggcc aacgccgttt ggctaattct gttcggttct aagaactgag aaagcagaac
     2581 gtcacccggc gttgccggtc ttaatacttg tgtctggttc atgcttcacc tcgataatca
     2641 aaacggtaca gattgaagtg taacctataa ggtaaacatg tcaaactgat ctttattttg
     2701 gattgcacaa tccaaaaata agataattta gctgaaatga cctctattta acataataga
     2761 tcttatcggc accacgtcgg gagggttcgc ccgaaaaaca ccacataagc gttaaaagtt
     2821 atagtttccg gcgcgaaaaa gagggatgat tgatgttgct ggtttgttac aaaaaagcgg
     2881 ttttctattt ggtagtgatt tttgattgaa aggcttattt ctatagcttt ttgtgatatg
     2941 tgttaagggg tcagtttggc gagaactaaa tcataaagcc aagcgagagc cgtagaagca
     3001 aggccaataa tgatgaaacc cggaaccatg gaacggtcgc taaaaatagc aaggcagaga
     3061 acaacgccaa taaaagctat cgctgatgtg gcaaccaaca acaggcgaac cggcaaacgt
     3121 agccacaaca tgatgagaaa ggttagggag cgcaaaacgc ttcctaagcc ttttgccgtc
     3181 gccccagcaa tccacttagc tttcgcagca ccactattat catcgttaga agcaaccccg
     3241 ccacccggac gaatcggaac aacattactc gtcggactcc ggttttggtt gcttaatcct
     3301 ttgaatgttc tcatcacaat cccctttttg tatgaatccc atacgttaat gataggccaa
     3361 aatggcctac ccctcaagaa atattcgact atttttcgtg catacgaagg aaaatcaact
     3421 cttccattat catcaacatg gtgtatattg attatcgaaa gatgcaattt tgataaaaat
     3481 gaattaagga gaagtaccat gaacacggta aaaattatcg ctgtagcctt ggggttgact
     3541 cttatttctg ccatgccagc ccaagcaaaa gacccttgta aagcggtttt atgcctcgcg
     3601 ggtatgctga ccggcaatga cggcggatca gagtgcaaat cagcagttaa tgattacttt
     3661 tcgatagtca aattcaagaa gggcaagttc aaaaaaagcg caactaaaga agctcgcggc
     3721 gcttttctac aacaatgcac cagtgaggat gaaggctgga ttaacaaaat aaatagcgcc
     3781 tacggcactg ttcgtaaggg tttctaacct tcttatggcg tttaaaacta aggggatgtg
     3841 atggaactct tttcaatatt ggcaacctgc gccgctttga cttttggtac aggtagccca
     3901 gcagaacagg cttaccctgt tagtgagcaa gaatgtaaat cagcagcggc gatgtatcgt
     3961 gagcagttgc aaaaagtaca gctcaccgat caggacaggg atgctattgc acgtgttgcc
     4021 atagccgaag cggccaatca aggtgattcg ggtttagctg gagttgtgta tacgattatc
     4081 aaccggcgtc ttagcggtca gtttggccag actgttacgg acgtagtaaa cagccctaag
     4141 caatttgagc cagttaccaa agttggtggt gactggaaaa agttaccttc cgtaaccgtt
     4201 tcacaatata cgcaagtaca gaccatcatc aatttggcgt tagaaggtca cttgcctgat
     4261 attaccaatg gtgggttatt tttccagaat cccgcaattg tagctgacag agaaaaagca
     4321 ggaaaagtat ctaaaggttt aactaatttc ggtggttccg caccatcagc gatcattaaa
     4381 gatcatgcct tctacgccag tattaataaa ggtggcccat ctaacgctcc cccggttcaa
     4441 ttaacagctt ccgctgtgcc tgagcctaaa aaatgggatg tatacggaac tgcaaagttt
     4501 caaaaaaata gtaacgccga atggaatgta ttttctaaaa atacgccatc caaaagcgtt
     4561 tatcaaaatg aaggggtgca gccatgaaat taatgattgt ggaatctccg gggaaagtta
     4621 agaaaatacg cgaaatcctt ggcgatggtt ggaaagtcgc cgccagtgtt ggccatgtca
     4681 gagatttacc agaaaaagat attggggttg ccgcacctga cttcaaacca acgtatgtgg
     4741 ccactgagcg cggcaaagag gttttggcca agcttaaaca ggacgttcag catagtgatg
     4801 ctgttttcct cgcaactgac ttagaccggg aaggggaagc cattgcatgg catctgaaac
     4861 aggcgctacg gcttgagaac ccgcagcggg taacgttcgc tgaaatcacc cctcgcgcta
     4921 tcaaagccgc tctggcgtca ccaagggcaa ttaatatccc tatggtagcc tcacaagaag
     4981 ggcgaagagt ccttgatcgc cttgttggtt acatggtttc gccgcgcttg tcagaaatga
     5041 gtgggcgaca agggttatcc gctggccgag ttcaaagccc cgccgttcgt attattcttg
     5101 aacgtgaacg tgctattcaa aactttaagc ccaccgccca ttttggtgta atacttaatt
     5161 ttgcaggcga ttggtttgct gaatgggatg taaaaccttt cttggccgaa ggtagcgaat
     5221 atttcatgga tgaaggcttt gctaaaaaaa tagccgccat ccgggaagtt gacgttattt
     5281 cttatgaaga gaaaaacgca tcaaaagcac ctcctgcgcc ttttatcact tcaacattac
     5341 agcaggcggc atctaataaa ttaaagatga agccaaaagc aacaatgaca accgcgcaaa
     5401 aattatatga gcaaggccat ataacttata tgcgcaccga taacccaaac ctttctgatg
     5461 atgctctggt tgaaatatac caactctgca aagctaaggg tttacctatg gcgtctaaac
     5521 ctcgtacatg gaaagcgaaa ggtgacgcac aagaagcgca tgaggctata cgaccatcac
     5581 attttgacgt tgaagaagca gggagtacgc cagaagaaaa agctctctat cgcctgatat
     5641 gggatcgcgc tgttgcctgt caattagaat cagctcgcta cgccgttcgc gtggcccgct
     5701 tcctggccag tgacaatagc gacggaaaac cagttgagtt tgaggctgaa ggtcgaacgt
     5761 tgacgtatgc cggatggaca acgttattta aagacgatac cgaggccgat gatggcaaaa
     5821 aggaagagaa acctaagaac ccgatccctt ctttgcaacg tggtcagcac cttaccgctg
     5881 aaagtgggaa ggtattaagt aagacaaccc gcgcccccag ccgctttact gaggcatcac
     5941 ttatcgcaga actggaaaga ttaggcattg ggcgaccaag tacctacgcg gcgatcatgg
     6001 aaaacatcat gcgccgtggt tacgtcaaat cggatagcgc aaacaaatat cttttgccta
     6061 ctgaaaccgg tgcattagtt attgatgctt tggttaaagg cggctttagc tttatcgagt
     6121 acgactttac acgcgctatg gagggcgagc tggataaagt tgcccatggc cagacatcat
     6181 acaaaaacgt tgttggctct gtttatggac gtctggaatc agaacttagc cacctgaaaa
     6241 ttgacgttgc ccctcaatac ccctgtccaa attgcggcaa ggctcttttc cgcaaaaaag
     6301 gggctaccgg cttcttttgg ggctgctccg gttatcctga ttgctctacc acgttaccag
     6361 atacagcagg gaaaccgggg aaaccaaagc ccaaaccaga agcttcaggc tttaagtgtc
     6421 ctgactgtgg aaagcctctt atccggcgta aaaaaccagc caccggcggc aaaaaagggt
     6481 atgacttttt cggctgttct ggctatcccg gttgctcatc aaaatttgat gctgtgaacg
     6541 gaaagccaaa attcgagaaa aagtaacatc aaaaagtagt gttatgatta tcaatatgct
     6601 atattttgat tgtgccggtg ggggaggaat gaagaaaaag actttttgcg taaagcattt
     6661 aaaatgttct cgaactttgg aatccccaat cgagaatatt gacatgaaca acaaaacgat
     6721 aacagaacca gaactgattg ataaccttgt gaatcagtca gtaaagaaga tgatgctggt
     6781 acagggtgaa gatggaaaat ttagcatccg cgtacagctt tcgtggaaag gaggagatct
     6841 tttattagag acacaaagga agacgccgcg agtgtgggcg agtttagaca ggttagtgaa
     6901 acacattaac agtaagtacg ggaaagtccc gatcattgag ttacaactta tggagtaatt
     6961 aaagcatggc aaatgtaaaa accttcaccc gtcagattgc ctctaccact tggctaatgt
     7021 tgggtttgat gatgttaccg ggtttcgctc acgcagccgg tggcgatcca attaaccaag
     7081 ggcttcaatg gttaattgat ctcttaacgt caggcattgc ccgttcttgc gcaatcttag
     7141 cgatcgccgt gttgggttac ttagcatggg ccggacgtct ccaagcccgt aatgccgggt
     7201 ttgctatcgg cggtatcgtg ctggtatttg gtgcggcgtc tattgccgac gcaatcattg
     7261 ctgtagtcca gtaaggaggg cgtcatggca tcaggaaaag atgttctctt tgccggtgct
     7321 acacgcccct cacttgtgtg gggcgtaaca tacgaggcaa taattttctg tatcgctaca
     7381 actggagtga tttttctcgc tgcaaatagc atttggttgt tgcctctgta tgttccttta
     7441 cacggtgctt gctacctgat ttgcctgaaa gacccccgtt ttttcaggct catatcttta
     7501 tggtttgcaa ctaagtgccg ttccattggg tggcgttatt ggggtgcggc tacggcctca
     7561 ccactcgtta gcactcgcaa aagaaggaaa atgccagaat gagtaatgca actgcgacaa
     7621 aatcacgcca atgggtgaat gaaaactctg ttgctgagtt tatcccttat tctgttcacc
     7681 ttgacgaaaa cactattaaa actaccagtg gctattatct tcaggtaatt aagattgatg
     7741 gccgcgccca tgaaagtgca gatccagaag atgtgattaa ctggaaagaa caacttaatg
     7801 ggctgctgaa aaatatagcc tcaccaaata tatctctttg gacaaacatt attcgccgtg
     7861 aagaaaatac atttcccggc ggggagtttg aagaaggtag ctttgctgaa cagctaaata
     7921 ataaatacaa aaatcatgtt ggtaaaaata acatgatggt aaacgagctg tatcttacag
     7981 ttctttatcg cccttcaggt attcccggcg ttaatctcct tcagcgtttt gaaaaaaatg
     8041 ttgaatctat taaacgtata caggctgaag ctattgaagg gttacacgaa gtggttaaca
     8101 ccgtgaccag tagcctaaaa cattatgggg cgcgggtttt agggtgttat gacagaaagg
     8161 gtataaaaaa ctctgaagta ttagagtttt tttcttacct gataaatctt gagtggattc
     8221 ctcgcgcttt acctcgcaag gtactctctg aagcgttgcc ttttgatcgt ccgttctttg
     8281 gtgaagatgc ctttgaagtt aggggcgtca ctgaaagcac cgtgggcgca gtccttagca
     8341 ttggtgaata tccagaaggc accgaagcgg gcttaatgaa tgcgttttta tccgcgccat
     8401 tcccgttgat tttgtcgcag tcgttcaact ttttaagtaa gccggttgca actgaattat
     8461 taggcagaac acaacgccga atgaaaaacg ccggggactt ttccaaaacg caaatggaag
     8521 agatcgacga cgcgcttgat gatttaactt caggccgtct tgtattcggt gaacatcatt
     8581 tagccctaac gattctggct aaagatgcac gaagcctgaa gaaaaatctt tctgacgcta
     8641 aagccgagct ggtcgattgc aacatgatcg tgtcacgtga agattgggct atagagtctg
     8701 ctttctggtc tatgttgccc ggaaacttta agtaccgggc aaggccagcc cccataagtt
     8761 cacgtaattt tgcggggttt tcctcatttc acaactaccc aacaggccgc agaactggca
     8821 accagtgggg gccatcggta acgctactta aaactacttc tggcgctccc tattatttta
     8881 atttccatag cgagttaagc gccgagaagg ccagaaaact agcgcaactg gaagcggaaa
     8941 agggggcggg tagcattcag gaaacgaaag aggaacaaaa agctctaggg aatacgttaa
     9001 ttatcgggcc ttcaggctcc ggtaaaaccg taacgcaagg tttcttgatg gctcaaagta
     9061 agaaatttaa ccctactcaa attatcttcg ataaagatcg tgggttagaa atctatgtaa
     9121 gagcagaagg cggcgtttat ttgcctttta agaatggtgc ccgcactggc tgtaacccgt
     9181 tccagatgga gccttgcgaa gatacttatc agctccttga gcagatagtt aaaaaatgtg
     9241 ccggtggttc cttcacgacc gttcaggata ctgacattag taataccgtt cgcggggtta
     9301 tgcgcctgcc gaaacactta cgccggatct ctgcctgcct tgactttatg gacccaacag
     9361 atccagaagg tgtgggcgct cgtttggcta agtggtgtgg taatggaacc ttagcttggg
     9421 tattcgataa cgaaacagac gacattgatt ttagtaataa cacgatgttc ggttttgacg
     9481 ttacggactt tcttgataac gccgagatca gaacgccatt aattatgtat ttgttccatc
     9541 gcatttctca aattattgat ggccgtcgaa tgatgatttt catggatgag ttctggaaat
     9601 tattgttgga tgaatatttt gaagactttg cacaaaacgg tctgaaaacc atccgtaaat
     9661 tgaacgggtt aatggtgttt ggcactcaat cagctaaaga cgttcttaaa tccgctattg
     9721 ggtacagcat cattgaacag tgcgcaacga tggtctttat gcctaaccct aaagctgatt
     9781 gggatgatta cgttaaaggc ttcaaactta cagaacgtga gtaccagtta atcaaaaccg
     9841 acatggcccc cggctctcat cagttcctta ttaagcatgg ccataattca gtgatcgcca
     9901 agctcgacct tgcagggttt agcgacgaac tagccgttat ctctggaacg gctgacaacg
     9961 tggaactatt ggaacgcatc atgcaacaag taggggatga tcctaaagtc tggttgccag
    10021 agttccataa acagcgcgta gctgcctgat tattaatgga gaatacaatt atgaaactga
    10081 aaaaattagc gttagtgctg gccattgcag gaagccttaa tgccgttagc tatgctggcg
    10141 ttcctgtgag tgtagttgca gacccgcaag catggacagc acatcttgag gacatggcca
    10201 aatatgttga gcaaattaaa cagcttgaaa accagtttaa acagcaggtt aaacagtatg
    10261 aatccatgac cagcgcacgt gggttggcta atgtaatcaa ctcccaatat gacactgacg
    10321 ccctgagtgg catcaatacc gacagcattt tacgtgatag cggcattaat tcagccagtg
    10381 attttaagct tcctgaagac gtggccgaac tttacgacac ggcagcaaaa aacgccgcca
    10441 cctatgctgg ccaagcttcc cgctcgcttg aacaagcaca atctcgtttt actgagctat
    10501 caggtttggt acgccgggta aatacggcaa ccgaccccaa agaagtgatg gatttaaacg
    10561 cgcggattgg ggccgaacaa gcctttttac aaaatgaagt tggtaagttg caggttctcc
    10621 agcaaacagc gcaggcaaac gatgcgttgt accaacagaa ggttaaacag atggccgttc
    10681 aatcatctgg ctcactgcgt aatgtgaact ggtagaggta actatgtttt tttcgtgcgc
    10741 acgaaaaata agcgcggtgt gtctggtaag ccttgcgctt accggatgct tcgacaaaga
    10801 agaaacgcat tccgttgaat ggttctctaa ccacaatgca gaacgggccg aaaccgttaa
    10861 gcgttgtagt gataaccccg gcgagcttgg caatacgcca aactgtaaaa atgccattgc
    10921 tgcggaacaa aaagcctcta gtggctcatt gcgccacatt aataattggt aagggaaggg
    10981 ggcggttatg ggtacgggaa caaacttatt tgtccgtatt tttgccaacg ttgactcggc
    11041 gttatcaacc tacattaccg atacggtcgg taatgtaatt ggttatgcct cgccactgtt
    11101 caccagcatg atgatcgtct ggattgccat gtggggcatt ctgatgatgt tcgccaaagt
    11161 tgatgaaccg gtacgcgatg gcgtctttcg catcattcgt attggtggga ttgtcgcgct
    11221 gggcttaacg gtcggtactt atatggatgt ggtcgttaac gtcctacaaa aaggcccgga
    11281 acacatttca gcggtggttt ccggtacaac gggaacaaca gccgacacgc tggatgcgct
    11341 ctttaccaaa atattcaatg tggccaaagc tgcatgggat aagggcgggg tcatgaacgg
    11401 aaacttcggt ttgtatctga tagccctgat tgtgctggtt gttgggtgtg gattgacgtt
    11461 gtttgtggcg ttcttggtct tgttgtcaaa gttcatgata accatcctgc tagggattgg
    11521 gccactgttc atcattgcac tgctatttaa caccacgcag cgattctttg aagcttggtt
    11581 agcgatggta tgcaactttg gcattctgct gattctttcg gcatcggtag gttcgctgat
    11641 gatctcgtta ggtgacacct acatttcaaa gatggcacca acagaaagcg cagcggccaa
    11701 tatggctaac ttaggggatg cggcgatgtt atgccttgta tttgcgcttt gtattttagt
    11761 ggtaaggcaa gttccttcta tcgcttcagc attaggcggc ggtgtggcca tggccacgca
    11821 aggcgcgttc ggctctgcca tgaatgcgtt acggccaaca gcaatgaaac gcggtatgca
    11881 gaacgttaac cgagaagtta agacgactca acgcgccatg acttcccccg ttcgcggtgg
    11941 tcaagcggct tatacctcct atcaaaaacg ctttggtggt aattcagtcg cacaaggata
    12001 gtagtgaagc cttttattgt acataaaacg cacaaatcga atgatgaagg ggaaattccc
    12061 ctcatctttt tttttgcgtt atgataatca aaatggtata ttttgattat caatgtaacg
    12121 caacttgata aggaacgatt gaatgaagaa atctctagca gtaatcgcac tcactttcac
    12181 ccttggggct tgcactctca cgccaccgcc accggctgaa gctgtagacg atgggcgcgg
    12241 catggttccg attaacccgg caatggttac gcaagaacag attaatgccg tcagctcgaa
    12301 gtacaaggac gtaaataaga aacaggttca gcaactgaag gagtttgcgc atggccaata
    12361 aaaaagcagc ccctgccaaa gaaaaaaaag taaccgacaa ggattacttt gatgaggcta
    12421 aaagctggga tgaatctgag attgtcagag aaaagaaatc agcccgtaga gcttggtcgg
    12481 cattttgggc tatgactggt gttgtaattg ttcaggcgat tgcaatttct acaatgatgc
    12541 ctctaaaaac aattgaaaat tcaattgttc gcgttaatga caccaccggc gaaactgagg
    12601 tgatcagcaa cctgaaaaac atggatgaga ctaccgaaca ggtgatgagc cgttactggt
    12661 tggctaagta tcttcgccac cgcgaagggt atcactggaa cacccgcgaa gatgatcgcc
    12721 ttcaggttgg catgttgtca gacagggcca ttcagcaaca gtacgctgat tacaccaatc
    12781 ccaaagttaa cccttacgcg ccaatcaaga tttatggtga aaccactgag gttgatatta
    12841 aggtcaatcc cgccattaca tacctaaacg gtaaaggtgg agtgaaacca gagaagggcg
    12901 agaaggatca gttcggtgaa acggtgtata cagcgttagt acgctacacg gctaccgtga
    12961 agaaagacgg tgaaatgccg gtaacaaccc attgggccgc tactgtctct ttcgtctacc
    13021 gtaaagagcc aatcaaggtg gatgatcgcc tgataaaccc ggttggtttt caggttatta
    13081 gctaccgtaa agatcaggaa gggggttaat tatgctttcg cttaataaaa cctccttatc
    13141 ggttcttctc gttctcggca ttttttcttc tacggcgttt gcgcttgaag ttccaagagg
    13201 cggcgcttat gacaaacgag tcaagttcat ttcatacaac catgagcaag ttgttaaatt
    13261 gattggccat tatggctatt caacagatat tcaatttgcc cccggcgaga acgtcaccaa
    13321 catagcaatg ggcgacccgg acgcttggga agttgcgcca gtagaaaacc atatcttcat
    13381 taagccaaca ggcgaacaag ccaccacaaa catgacggtt ttaactgacc ggcgcgtata
    13441 cacgtttgat ttatccgcta ctggtaagaa aaccggcgat atgtattttc aggtaaattt
    13501 ccgttatccg caggacgaag cggcgcggcg tgtagctgaa gaagatgctc gcagactgaa
    13561 agaacggatg aatgaaaaag cgcctaacgc ttggacaaac tggaactatt gggtattcgg
    13621 ttctcgtgaa gtctcccctt ggcgtgctta tgatgatggc cgttttacct acttcaagtt
    13681 ttccggtaat acctcgatcc ccgctatcta cattaaacat gctgatggct ctgaaacact
    13741 tctaaaccca acgatggatt ccgatcaccc ggataccatg attgttgaaa aagtggctcc
    13801 taggtttgtt ttacgcaatg gcaaatatat tgccaacgtc tacaacgaat ctttcaaccc
    13861 tcatggatta atcaatacca gtggcacaac gtcacccggc gttaagcgtg taattaaggg
    13921 aggggcgcag taatggctaa agaccgcgac gacttagata cagaaatcga acaggaagat
    13981 gctgatcgtg gtatgccttc gatttccaaa aacaggaaaa ccggtaaagc ctccggtatt
    14041 ggtcagaaaa tcgtagctgt aatcataggg attatcgtta ttctggcgtt aatagcggtg
    14101 aatggtggct ttaaaggtag tgaagaagat aaaccgccta agctcactgg taacaccagt
    14161 gtagccaacc ggcttggccc gccgccttct ttaccaccgc cgccgccagc tccaccaaat
    14221 actgagacgc caaaagaaga tcggatgttg aatacagggc aaatagcccc gccgccgccg
    14281 tcttcagctt cgggaactgg tggcaatggg aagcatgaac agacaccaga cgaacgcaag
    14341 cgggcaagct ctattctggc gtttggcagt atttctaaac ctcaacagcc cggacaaaaa
    14401 gcaggtaatg gccagcccgt tcaaatggcc ggtgaggtac aggagggtaa tagctcacta
    14461 tctgaaactg accgcgctga tagtctttct aaccggctta aaggtactga ggttagcggt
    14521 agcagggcaa gcctgttagt agacagaagt tactttatca cacaaggcac tttccttaat
    14581 tgtgcgctgg aaacggctat tagtagtgat gttcccggaa tgaccgcctg ccgtttaact
    14641 gatgatgtct acagtacaga caagaaagtt ttgctactgg aaaagggttc aaaaattgtt
    14701 ggccagtatc agggcggcat aaagagagga caggcgcgga ttttcgttct ctggactcgt
    14761 atagaaacgc ctaaaggggt gttggtcaaa ctggattcac cgggtactga tgcccttggc
    14821 cgctctggtc ttgatggctt cgttgatacg cacttttggg aacggttcgg tagcgccatt
    14881 atgttgacca cggttgatgg ccttattacc tacggggcta atagcgccaa caatggcagc
    14941 aatggcaacc aatacaactt tggtgatacc gcgcaaacag cgcgagattc agcgagcatt
    15001 gcgctggaaa actcgatcaa cataccgcca acgttaacca aaaatcaggg tgacaatatt
    15061 tctatctttg tagctcgcga tctcgacttc aggggggtgt atgaccttaa agcagcctac
    15121 taatgctaaa ggttcatcac ttttggagta ccttcgggta ctccagccct ttttaaacga
    15181 agacggggtt actgaagtgg tggttaataa acccggagag gtaattactg aaggccggaa
    15241 agggtggcag tttcataacg taccaaagtt agattttgct gcttgcgctg acatatcaaa
    15301 gcttactgcc acttattctg gccagtcatt tgacgagcgt aagccgattg tttccgcaac
    15361 attaccctac ggcgaacgta tccagattgt gcggccaccg gcgacactga gcgaccgtta
    15421 cagcctgact atccgtaaac cctccgatgt gatcagaacg ttggatgatt acgagcgtga
    15481 cggtgcattc gatcacatcg taacggacgc tgtagggctg cgtgactttg agcgagaact
    15541 tattgagctg aagaacgcca gacgctttaa agagttcctc acgctggcga ttaaacatcg
    15601 ccagaatatt atcgtttccg gcgctactgg ttcaggtaaa acaacgttca tgcgttcatt
    15661 gcttcagctt gtacccgatg atgaaagact gatcaccatc gagaacgtag acgagctgaa
    15721 gctataccaa acacataaaa acgttgtgcc tctgttttac agcgccggtg gccaaggtat
    15781 tgctgatgtg acccagcagc accttttaga aagttctctt cgcatgaaac cagatcgtgt
    15841 gtttgttgcc gagttgattc gtggtgatga ggctttctat tacctgcgta acgttaacag
    15901 tggccatcct ggatctataa cgtctatgca cggcactaac ccacgaatgg ccatagaaca
    15961 gcttgttttg ttcattaagg aaagccaaag cggcggaaca atgtcacgtg aagatattaa
    16021 acagcttctg tttagttgca tcgacataat tgttcaattc caaaaccatc aaggccgtcg
    16081 atatatttcg gagatctatt atgagccaga gttcaaacaa aatattttgg tggcttaaaa
    16141 tcctgctgct gattagcttg ccgcttatct ttttggcggt agcggcttgg attgctgggg
    16201 ctatattcat ggccggtaat ggatacgagg ccaaagatgc gacacccctg acgctatacc
    16261 aatattggta ctattacggt gatataccta aaactaagtt atggctgatg gggtctttag
    16321 ttggcggttt aattattacc gcgttgcctt gcttgctgcc gcttctgccg aagaaaaccc
    16381 cgttgcatgg taacgctcgc tgggctaaca gtcgtgagat ccgcgaggct ggtttattca
    16441 gtgatgatgg tattatcgtc ggcgtaaaaa aaggctttat gggccttttc atgcagtacc
    16501 ttgtattttc cggttctcaa cacgttttga tggccgcgcc aactcgttca ggcaaggggg
    16561 ttagtatcgt agtccctaac cttctttcat ggcgtgatag cgttgtagtt ctggatataa
    16621 aacaggaaaa ctgggatata acttccggct ttcgctctcg tcatggccaa gactgttttt
    16681 tgttaaatct tgcgccaagg gattaccgtt cgcaccgctg gaacccacta ttttatattt
    16741 ctgacgatcc caattttcgc attaacgaca ttcagaaaat tggtcagatg ttgtttccca
    16801 agattgagaa tgaagcgcca atttggcaat catcggcccg ttctctttgg ttaggtatgg
    16861 tgctttatct catcgagacg gaagagctgc cggtaacaat gggtgaagcc cttcgccagt
    16921 taactatggg tgatgaacgt ctggctgaaa ttgtcgaagc cagacaagaa tctgaaaccc
    16981 ctttatcaga tgaatgttac ttagctctaa aagaatattt agatacgcca gataaaacac
    17041 gtggttctgt ccgtaaaggt tttacttcgg cgttagaact tttctataac ccggtgatcg
    17101 atgccgtaac cagtggtaat gactttgatt tgcgtgaact gcgtaagcgc cgcatgtcta
    17161 tctatgttgg cattacaccg gacgatttag aacgtttggc cccgctgatt aacttatttt
    17221 tccagcaagt catagacctg aacacgcggg aactgccgga gcaaaatccc gaccttaaat
    17281 atcaggtatt gatgttaatg gatgagttca cggccatggg caaagttaac attctgtcaa
    17341 aaggcattag ctatgttgcg ggttatggct tgcgaatgct gccaataatc caaagccccg
    17401 cgcagttacg cgaaacctat ggccatgatg cagcggaaac atttattgat aaccatgcgt
    17461 tacaaatagt ttttgcgcca aagaatctta aaattgccaa agaagtttca gagtctcttg
    17521 gtactaaaac agtgaaagcc agatcccgca gccgttctac tattgggagg gcaagcggtt
    17581 cagaaaatac cagcgaaact ggccgcgcgc tgttaatgcc tcaagaactt aaacagatgg
    17641 ggaaaagcaa agaaattatt ctgttagagg attgccctcc gatccaatgc tctaaaatta
    17701 cttggtataa agaccccact tttgaagcca gaggtaatgg ccgtgatggc gttaaattcc
    17761 cttccccgga agtgacaatt attgacccag ccaacaggcc aaaaggtgaa gtttccttta
    17821 tgagcaaccg ggttgaacag gctgaagaga caaaaaccga agttgtggaa cgtgctgtta
    17881 ccgttgctga tattgaaaat atcgacaagc tgaatcttga tgattttagt tgtgattttt
    17941 caaaggttga aatccctaaa ggggatatta ccgatgatgc gatggacgac atagttaatc
    18001 aatttttctc tggcttagaa gctgcttaaa ggagtaattc actatgaatg aaccaaactt
    18061 agcaactaag aacaccgtcc ttatcgtcag ttttgaggaa aggaatgaag ctaaaaaagc
    18121 ggctggcaag ttagagaacg gcgaaaacgc tttaggcttc gatgaggaag cgaaacactg
    18181 gtttgctaaa ccgggtgcag acctgagcaa gttaagcaaa tggctaccag atcagcataa
    18241 aggtaatcca gtacaggata ataatccggc tgctgaattt ggcgctacgt tagctgaagc
    18301 aggctttgaa ctggatggcc tgccggtgat ggatggtaaa aagcaccgag tcagaacggc
    18361 tgacgataaa aaaggcgaaa agaccggggt ttatgcgggc tatttagacg gcgttccggc
    18421 tgggtggtat caggatcacc gaatccatga ccagcccgtt aaatgggtat cgactggcca
    18481 gcaagttgac gccgaagccc aagcccattt acgtgctatt gcggcccaaa accgcattga
    18541 tagagagaat gccttacaac gacagtatgc gcacaacgcc cggagatctg gccaagtata
    18601 taacgcgatg ccggttgctg acggtagcca agaatatttg gttaataagg gtgtaaaggc
    18661 attccccggc gttaaggctg ataagaaaaa ccgggtggtt attccactgg tcaacgaaga
    18721 aggcgaagtt cgtaccctgc aacgtatcag cgccaatggg ttcaagtcgc tgaagaagaa
    18781 cggccagaaa accggtaatt tttttgttgt tggtggtgaa ctgaaaaacg gcgagccggt
    18841 tctctatgct gaaggttact caacggcggc cagtattagc gaagccacca atcgcccggt
    18901 agtaatgacc gttgacgcgg gaaacctgcc taaagttgcc gacaagctta aagataaata
    18961 cccggatagc ttgcatgttg tgcttggcga cgacgaccgc aaaaatgccg ttaacaaagg
    19021 gatggccaaa gctgaagaag cggccactat cgcaaatggt gttttcaccg taccggcgtt
    19081 taccgctgaa gaaaaagaac gcggtttaac tgactttaac gaccttcacc aatcacgcgg
    19141 gcttgatgcc gttcgcgatc aggttgaagg aatcattact gaacatactc ataaggctga
    19201 aacaatggct gataaagaac atgaggcaat aaatacaggc ccggtgatta ctgaaaattt
    19261 tgaaagggaa atggacgctg cccttaatgt gaatcattcc tctatagatt tagagccgta
    19321 tcaatctgaa gaggatgagt atgatgtaga aaacagcatc gagccacata tagaacagga
    19381 aaaggcccaa acggctgatg atgatgtaag agcatggtta tcatcatcac gtgtttctac
    19441 tgcggatatg ttagctgaag acgatgaacg cgagggccgt aaacgcgaag agttcaagca
    19501 agtagagcct gacgctgaag agccagtttt taaacgtcag tcgcaagatc aggattattt
    19561 cgaagacagc tcttataaac ctgtagtgcc agatagagtg gccaagtctt atattgaggt
    19621 tgaaggtaaa tattatttcc aaaatcgccc ggactcactg gcctttgtgg acaaaggcgc
    19681 gaagcttcaa accaaactga gcaatagcca agtagccggt tccatgattg atattgctga
    19741 agcacgtggc tggactgaga tccaagtaaa aggtactgaa gattttcggc gtgaagcatg
    19801 gttacaggct acggccagag gactaaccgc ccatggctac aagccaaaag aagaggattt
    19861 agcacggctg agaaaagtag ccggtgagcg caacattaac gaagttgaag cgcgtgaagt
    19921 tgctgataac gtgaaaacgt caccttctgc cccttccggt acaacgtcac aaaatcaatc
    19981 cgaagcccct acatccaaaa ccggcaccac tgagtcaagc agtacggcac aaggcaaaaa
    20041 tgagggtgct aaagttgaag ctgaagaaaa ggttaacaag ctggccggta agttggtaga
    20101 gcatggcgcg gccccttatg aacataagaa agacaacaaa gataattact atgtaacgct
    20161 ggaaaacgcc gatggtaaac aaagcacaac atggggtgtt gatttacaac gtgccatggc
    20221 cgagagtgaa gctcaaccgg gtgatcatgt tgagttagaa aaccttggac gcaaagctgt
    20281 taccgttgaa aaagatgtta aagacgaaac gggcaaagta gtcaaaaagg aaactatcaa
    20341 tacgttccgt aatagctggc aggttaaagc tgatgccatt cgtgatcagg aacgcccagc
    20401 gcgagaaata gttaaagaac atcccgattt agtaaacgaa attacagcgg ttaaactggc
    20461 tgagaaagta tctcaatcaa tgaacgctga cgacaaagaa cgctttatga gccgcgtccg
    20521 tgacaaacta gcggataaag tggccagtgg cgaaaaaggg ccagaactga aaatcaaaga
    20581 ggttcaaacg gccgataacc cacgggttaa agataccgaa ctggaacgct gatttatcgt
    20641 taactaccat ggcccagacg cttttagctc cgggcttttt tatagggtaa taggcatgaa
    20701 caataaaata ctggatctga atataccggg ccgtgaaatt gagatgagcc gccaagaagc
    20761 tgaagagtta ggcgcatttg aagaaagcgc cttatctgaa actgatgcaa gagaggcgat
    20821 tgatatagaa gacgagcagg agggttaacc gatggccaga ggtgacgaca aagagaagaa
    20881 accattctat gaagaaattg ctgatcgtct gatcaaacag cttgaggaag gaaccgcgcc
    20941 atggcaaaaa ccatgggaac cgggttcaag cagaatgccg cataacccgg ttagcggtac
    21001 tcgttacaag ggagccaacg ccctatggct ggctatgcaa cagcgtacag atccccgctg
    21061 gatgacgtat aagcaagccc aaagcgtgaa tgcgcaggtt caaaagggcg aaaaaggtac
    21121 gctggttcaa tactggaagt ttaccgacac aatccccaag ctcgacgata aagggaaacc
    21181 ggtactggat gataacggta agaaaaaaat gattaccgtg aaacttgatc gcccaaaagt
    21241 cttttccgca gtggtattta atgcggaaca gattcaaggc ttaccgccat tagaagttaa
    21301 ggcaacaaaa cccgattggg aacgccatga acgagccgaa aatatcttgc aggccatgag
    21361 cgtacctatc aagcatgatc aagctgatgg cgcattttac cgcccttcta cagattcgat
    21421 acacctcccg gaacgtagcc aattccccag cgccgataat tactatgcca ctgcattgca
    21481 tgaagctggc cattcaaccg gacacaaaag ccgtctggat agagatttaa ccgggcgatt
    21541 tggttctgag tcctacgcaa aggaagaact gagagctgaa atagcctctt tgatgattgg
    21601 cgatgaattg caaataggcc atgaccccgg acaacacgcc gcttacgtta aaagctgggt
    21661 gaaggttcta aaggatgatc ccaaagaaat actgagggca gcaaaagacg ctgaagctat
    21721 tagttcctac cttttgagcc atgagaaaga aaaggcgcag gaagcccctc aaaacgcacc
    21781 agaaagcgat acagaccaaa aagagggtaa tgatagcgca ttgcctttaa aactcgctga
    21841 gaagcttgca gagcgtttta acaacgcaga agatcgggct aaattcatgg aaaagataca
    21901 gtccagactt acccacgaaa aaccagatac ggaaataaaa ataaaggaaa ttaatacggt
    21961 tgtagtaagc aaaaaaggtc taaaacaaga tactgattta gagcgttaaa aaacaggcgg
    22021 gaaatagccc gcctgtctcc cctcccctgt gcggtttcgt gcgcacgaaa tttatcgttc
    22081 ttcctgatcg ttatctctat ctctgccagt tgaattttgc ttttttccgg gctgctggct
    22141 tgctagtttc tgactaactg ctttggccat ttcatgtcgt tccgtagcca taggaggcat
    22201 ggtattaaca aaattcacaa catcagcggc aagcttttta tcgcccggct ctgaactctg
    22261 gttaagttcc tgcgccgccg atagccaccc ttttctaact tcctgctgcc ttgtctgtat
    22321 tttcttttcc cacggtttcg cttttgcagg ctttccggcc ctcgtatctt taaactcttc
    22381 tacgatctct ttgattttgg ctttatcgac ttccggggtt aatccacgct tgcgaatatg
    22441 aagaatcgcc tgatcaatac cttttttaac tacgccacgt gtagcgcgtg gtgttgcttc
    22501 agcttcaact cccctagcgc gaagttcttt agcaaaacct tcacgccata tttgaggatc
    22561 gccttttttc acatgtaagc gcctgccatc gaaacccaag gtttttatac tgatatgaac
    22621 atgcgggtga tcaacgtctt catgaagtgc aaaaacatac tggtagtttt cgccaaattg
    22681 tttttttgca aatgccctag ccgattcttt gacggcctta gcttccgttc ctttcggcat
    22741 ggatagcact acattagtag tatcgcgcgt attggcacgt cgcttacctt gatcctgagt
    22801 ccattcctta tgaacggctt taacgtcgtc ctttcccttc aatatttcac cgcgttcatt
    22861 ttcaagttcc agcttcccgt ttcggctgat gtaatctaaa tgggctttaa cgtgagctgg
    22921 gccttttgca ttgccactca ccttaaccat cacttcagac gcacgattag ctatgcgact
    22981 agccttactt ttcaggtctg tagttttgtt ggatttagtc tttactttac cctcaaggag
    23041 ttttgacaag ctttcaacgg ccatggttaa cctccccaac gattcatgtt tctatcaata
    23101 agattagata ccttgtctcg atggccatct atctgttcaa gcagccgatc tattaattcc
    23161 ttgttcagct tatcgctatc acgaaactct acattcaacg ctctggctat ctggttcagg
    23221 tttctaccta tcgcggccag ctcacgattg gaaccccgca aagcattaac ttcggcgtca
    23281 gttaataccg gttctttgct caaggttgcc aacactaaat tagtaagcca tgtggttctg
    23341 ctgggaaacc cttcagcctt agcgcgttct gtcatgctgt gaatatcttt cggtgacaaa
    23401 cgtatcgtta ctttatctga acgtggttct tccaatcctt tagcttcatt aggcactatt
    23461 cccgccgttg ccttttcgag caacagcccc aacatatcgg attcactaac accacgctca
    23521 tgacaaaagg ttttccactt ctcttttaat tccggcgtaa ttctcttaac gatcctcggc
    23581 ataagcgggc cttttacaat atggggggat ggggggtatt atagcaaagc cggtgccatg
    23641 cgccagcact ttgcgaatcc tgccctagct ataacccccc atatcctttt cgtgcgcacg
    23701 aaaattttgc acgaatggct atttcacgcc atcggctgcg ccttggccca aaatcaaaac
    23761 cggctcaaca ggaccagggg ctgatttgcg gctattgttt tggattgtgc aatccaataa
    23821 caaagagcga gatatacgcc aatgaaacct acacaacaga cttacgaaga attgcaggat
    23881 ggcgagttga cgataaagaa tgagaataaa tcaaacagga ttaaatacac ctgctctatg
    23941 tgcgaaacaa atatttgggg gaagcctgac ctgaatgtga tgtgtggaga ctgtaacgta
    24001 gcctttgaag tcgctaactg acgcgcttcg cttgtcggct attgatggaa ccgtaacaga
    24061 ctcgctacgc ttgctagtta cggttccatc aacagcgaat tttaacgcca tagataaatc
    24121 aattgtattt tttgaacaga aaatcagcca gcaacatagc tgagggggaa agtgtcattg
    24181 ttaatatgat ggcaagagaa ttttcgtaga cggtgctgct gttctgcaat gggtaggtga
    24241 cgaattgaac tatcgaagga ttccaattca tcaaccaact tttccagatc tccattcatt
    24301 atttccccct ccttaaaata cctataaaag tgcttttgat aaatccgggc ttacccttct
    24361 ttcctcgcag ccaactaaat aagttggctg gtatagctaa tgccaataca gcaaacgtta
    24421 cgcaaatagc tactacagcc aaaaatgatt ggctgttttt caggtaggga tacgccagaa
    24481 aaagaatcag gactaaagcg gcgataacgc cgctgattag cactatgcga tttttcataa
    24541 ttgcccgcct caaccttcaa caatggcaac aacgctaacg tcctttaaat caatctcgtc
    24601 ttcactgcca tcatcatcgt atttaatcca accgaaccca taagaagctc ggcggttcgt
    24661 gagtaatcgc gctgggcgtt catcatattt aatctgaaca atcgcctttt taattttgtc
    24721 gcttgcctca tctgcggcaa tgattttact ggcttcatcc tccaaatcag aagatgagcc
    24781 gccgctgatt ggttcgtcgt caataactaa aggttgttgc tcttcgccct tctccagttc
    24841 gtcgctatcg gcaccccctc ctatccatgc gttggcatcg ctcggcagct cctgaccttc
    24901 ttcattgtcg cctgacgtat tcacggcttt agattccggc tctttgagtg aatcacaaaa
    24961 agcccgcaca tcagcagtag ttagctcgtc ttgtgcttcg gaacaaaaag cctctacttc
    25021 ttcagcgtgt tttttatagg cacgatgtaa cagggtcatt gcctgcatag agacaacacg
    25081 gccactgtcg tacatatcgc ggatacaatc agccatatca aagaaaataa tatgatctga
    25141 tacaaacgat gctggcttac ccaatcgggc cgcgatggcg ctttttttat cgcctttctc
    25201 aatccggcgc tggataaacg cagcggtttc aattggtgaa agagctacgc gctgaatgtt
    25261 atcaactgca ttatcaaatt cgtcggcatc ctcatcaatg aacgccaaaa catcaggtaa
    25321 ctcggcaagt tcagcagcag cacaacggcg ctcaccggca ttaagaatat attcccccgg
    25381 tatatctgga tgaggtttca gacttaccgg gtttttgtta ccgcgtggtt ttccagttgc
    25441 cggatttata gacttcattg aatcggccag ctcacggagt tcctgcgggt cgatagtctt
    25501 tctggcgtta tctgcatcct tatgaatttt tttcagaggc acacggataa gatttcctgc
    25561 aaaaccttca ggtacgtctg ttgaagccgc tgattgagta ggggccgggt cgcctcccat
    25621 caaagctgaa aagttatcca gcccgcttaa atccaatcct tttttggcgc tcatttggca
    25681 acccccattt tttcaaaaat gatcttaaaa gcttcttgaa attcttttcc tgcctcacgt
    25741 gcagatgttt tctttaactg ccataccgga ataccttcgg aaagggcttc tggaattgat
    25801 gaacgaatgc cgatccgcgc tctaattaac agatgagcgt aattctctac cagattcatt
    25861 aatgtttttt tctggtcttc agaacgggag ttaaagcgat taggtaacat gcctaggaag
    25921 ttcagatctg gattccattt caacttaaca ccaaaaatgg tttgcagcat ttttgtaata
    25981 ccatcaatgg aatattcttc cagctctatt ggcgacaaca catgatttgc aacgataagc
    26041 gccgctgaca tacgcaaacc aagggtagga ggggtatcaa tgacacaatg atcaaactga
    26101 gacgccagcg cggcaaggtg gtctttaaag gttgacataa ccgttaacgg ggcgcgttct
    26161 atatcggcca tctttgcatc agcattgatt aaagtaatgc cttcatttgg cgtaatagtt
    26221 aactgttgtt cttcaaaaag ttggcttgcc tgaagtgccg ccacacctcc agccttttct
    26281 aaagtcttac tggcgttacc ctgcggatca agttcaacaa aaagcactct gtcgcctcgt
    26341 tcctgaagag ccatagcaag atgtacggcg attgtagatt tacctacccc gcccttctga
    26401 ttcgtcactg ttatgatatt cattcatttc acccttagcg tttgcctgtt tacgcgcctc
    26461 atcttccata gcccttaaca taggaaccat tgatgatggt atacatagct cgatacgttc
    26521 ccagcctgcc ggtggtttgc catgtaactt gtgcgcttct ctaactttct tcgtcattgc
    26581 cgctattgct gtacggctta atcccgtttc aatcaccatg tcattttgct ttctgccatc
    26641 aaccaaaatt gctttcacaa tttctacatt tgctggcttt agtgcgcgtg acaggttaga
    26701 aatggcatgt tcaaattcgg cttgcgttag tcgttttttc attcaatgga ttaacctccg
    26761 aaactcaacg gcctaactgt ggcctcccct taaatataaa tcatcatctg ttgatgtcaa
    26821 tagataatga ttcattttgc ggtttcgtgc gcacgaaatt tattgttaac tcgccgccat
    26881 ttccaaggct ctacaatgct tgattgcgga tcagaccata ggccaaggcg tttttgccta
    26941 gcgtcctgtt caaggtctag catggctgga ttagtcgcct gttcacggta tctgtaggcc
    27001 cacgccatac cgttactaac ctgagattca tttacgttgg tgttatcttc cttaaaaact
    27061 gtacctaaca cgcggttata gcgatccttt cccttttcaa cgatggttac ttgttgacga
    27121 aagatcatat cagccagaaa ttgacgggat ttctgcccga aaggttgctt gctttcaggg
    27181 gcatcaatat caactaatct cacccttatt ggcgttttat taactaaaac atcaacggta
    27241 tcaccgtcga taattcgtat tatttcgcca tgaatatcag caattgcata ttgcaacggt
    27301 aaaaataaga atagaaaaat aatattccaa cgcttcaaca ttaattttcg cctctttgtt
    27361 catctgattc ccaaataaca ccgaacccgg ctttataaca aagtaacgcc catgccgcgt
    27421 aaggtatccc gccttcttta caccaacgcc taaatgtccg gcttcccttt cctctccctg
    27481 tttcacttgg tagacctatg tattttgatg cgtcaacacc agtaaactta tttcctgatc
    27541 gctcttctac cagacttaac accgctttga tttcttcacc tgtcggaggc gtccaaccat
    27601 cactaaaagg gagtaaggtt tcagttctaa ttacgttgtt caaagtgtct ctcctgaaag
    27661 ccccgccgaa gcggggcaat aggcttagac agcctgaaag cctgcgttct gctcgcgtaa
    27721 tgcttcca
//
