LOCUS       NZ_CP038427             2728 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli O157:H7 strain 2571 plasmid p2571-2, complete
            sequence.
ACCESSION   NZ_CP038427
VERSION     NZ_CP038427.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11229478
            Assembly: GCF_013168235.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Bono,J.L.
  TITLE     Comparison of complete closed STEC O157:H7 genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Bono,J.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2019) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 85x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 03:24:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,703
            CDSs (total)                      :: 5,566
            Genes (coding)                    :: 5,262
            CDSs (with protein)               :: 5,262
            Genes (RNA)                       :: 137
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 304
            CDSs (without protein)            :: 304
            Pseudo Genes (ambiguous residues) :: 0 of 304
            Pseudo Genes (frameshifted)       :: 120 of 304
            Pseudo Genes (incomplete)         :: 166 of 304
            Pseudo Genes (internal stop)      :: 69 of 304
            Pseudo Genes (multiple problems)  :: 43 of 304
            Pseudo Genes (short protein)      :: 4 of 304
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP038427.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="2571"
                     /serotype="O157:H7"
                     /host="bovine"
                     /db_xref="taxon:83334"
                     /plasmid="p2571-2"
                     /geo_loc_name="USA"
     gene            complement(join(2435..2728,1..690))
                     /gene="mobV"
                     /locus_tag="E3150_RS28245"
                     /old_locus_tag="E3150_29155"
     CDS             complement(join(2435..2728,1..690))
                     /gene="mobV"
                     /locus_tag="E3150_RS28245"
                     /old_locus_tag="E3150_29155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852935.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_097223437.1"
                     /translation="MANFAIMRCKKLTGFGSVAGALKHCYRERETPNADAGRTPENARF
                     VSETSDQAMGRLREMLPEKRRRDAVLCVEYVMTASPEWWETADQKQQTEFFSRSVQWLT
                     DKYGEGRIIAAVIHRDEETPHLSAFVAPLTQDGRLSAKEFIGGRAKMRDDQTTFAASVR
                     ELGLERGIEGSRATHRTVQHYYESVNRGIRNRVDISPAALEPRVLKKGLFTKVIETPEN
                     VAARLSEAVNDGFAGTVAVASQSAANAKRARELHQTMEIQQKRVQDITEPFKGLSREQM
                     DEILGMASRFREQNREEQLRKRNRIREMFREKTGRDRQHTGRDWKR"
                     /name="mobV"
     gene            1258..1791
                     /locus_tag="E3150_RS28250"
                     /old_locus_tag="E3150_29160"
     CDS             1258..1791
                     /locus_tag="E3150_RS28250"
                     /old_locus_tag="E3150_29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279566.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_097294465.1"
                     /translation="MSFSRYKINPFLEDMIVPVKGKQVRLSRLGRDENILVNQSTGEIQ
                     GTHVATFKRVDGEQFVKLFTANIGLTFELSSAGIKAFGVLLWAVQNKALSKDEVDLDSF
                     VLEDFLEAQGATNKQPLRLSLATFKRGINELEKAQIVAKTMRQGRYFINPNFVFNGDRI
                     AFTTVIERQQKIEE"
                     /name="replication/maintenance protein RepL"
     gene            complement(1909..2388)
                     /locus_tag="E3150_RS28255"
                     /old_locus_tag="E3150_29165"
     CDS             complement(1909..2388)
                     /locus_tag="E3150_RS28255"
                     /old_locus_tag="E3150_29165"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_097289629.1"
                     /translation="MIKLKRVLSGTIFFVVLTNQVFAQTKYNSILSLPMALPDEKTINI
                     SNLPKISEWYQSCKRSEDIKNKSDWGFCSAYLYAVMQHHYDTSLKKCPNISVQEVLNAV
                     DKEALEYQEMYSKPAKGRDGKLIEGARTYQPNTIYEWNRPAFSVLSSVLEKECIL"
                     /name="hypothetical protein"
ORIGIN
        1 tgcctcactc agacgagcgg cgacgttctc cggtgtctca atcactttcg tgaacaatcc
       61 ctttttcagc acgcgcggct ccagtgcggc agggctgatg tccacacgat tacgtatccc
      121 ccggttcaca gattcgtagt agtgctgtac tgtccggtgc gtggcgcgac ttccttcaat
      181 cccgcgctcc agtcccagct ccctcactga cgccgcaaat gtcgtctggt catcgcgcat
      241 cttcgcacgt ccgccaataa actcctttgc cgacaggcga ccatcctggg tgagaggcgc
      301 gacgaacgcc gacaggtgcg gcgtttcctc gtcacggtgg atcacggcgg cgataatcct
      361 tccctctccg tacttgtcgg taagccactg cacagaccgg gaaaaaaatt cggtctgctg
      421 cttctggtct gccgtttccc accactccgg gcttgctgtc atcacgtact cgacgcacag
      481 caccgcatcc ctgcggcgct tttccggcag catttcgcgg agtcgtccca tcgcctgatc
      541 tgacgtttcc gagacaaatc gcgcgttttc cggtgtccgg ccagcatcgg cattcggtgt
      601 ttctcgctct ctgtagcagt gcttcagtgc acccgccaca ctgccgaatc ccgtcagttt
      661 cttgcatcgc atgatggcaa agttcgccat gcttcccccc ccctcgttcc ggcgggccac
      721 tatcagattg atagtgtact tactagactt tgcttcgcaa aatcgttcgg gcagcaagct
      781 gcctaaaggt caagttcaac agcggtctgg caggtggaag gcgtcgctcc gcgtcacctc
      841 ccaccactgc caagcctgtt ttatccggtg aatcgtcaag ggtaaatggc actcgcttcg
      901 ctcgcccttg acgaaatcag gcaatgaata tcgtttttga aagagcaaga accgctcgcc
      961 gcaagaacgg attagggagc ggcatcgccg cgagtctgtc cgatcgagga agcggaattt
     1021 ccactccccg gacatagaaa tccgtacgct ctagactgac agaaaaccgc ctgattaatt
     1081 tttttcgtta agaactaaga aggggatcac tctcagcccg catgaaatgg ggatcgtatt
     1141 ttcaggatct gtactacggt agctcaaccc tagtactacg gtagctcaaa aaaaaccttg
     1201 attttgagct accgtctatc taaaatcgaa tcctgtcgat catttaaggt ggttaaaatg
     1261 agcttctctc gctataaaat aaacccattt ttagaagata tgattgttcc agtcaagggg
     1321 aagcaagtta gactttcgcg tcttggtcgt gatgaaaaca tcctagtaaa ccaatcaacg
     1381 ggggagatcc aaggcactca tgtagcgact ttcaagcgag ttgatggtga acagtttgtg
     1441 aaactcttca cagcaaatat cggtttgacc tttgagctat catctgcggg gattaaagcg
     1501 tttggagttt tgctttgggc tgttcagaac aaggcgcttt caaaagacga agttgatttg
     1561 gatagttttg ttttagaaga ctttcttgaa gcccagggag ctacaaacaa acaaccacta
     1621 cgcttaagcc ttgcaacatt caaacggggt attaacgagt tggagaaagc gcagattgtt
     1681 gcaaaaacaa tgaggcaagg ccgctacttc attaacccaa attttgtttt taatggagat
     1741 cgaattgcat ttacaacagt gattgaacga caacaaaaaa tagaagaata attatatcaa
     1801 taactctcta ttatagattg attgatgcgt caagctatgt gcctgcctta aaatacaggc
     1861 actcccagag ttaacataat tcaggtttat tacgtataga ccaaataatc acaatatgca
     1921 ttccttttcc aaaacagaag ataacaccga aaatgcaggt cgattccatt cgtaaatagt
     1981 gtttggttga taagttcttg caccttcaat taatttaccg tctcttcctt ttgctggctt
     2041 tgaatacatt tcttgatatt ctaacgcctc tttgtcaact gcatttagaa cttcttgtac
     2101 tgatatatta ggacactttt ttagtgaggt atcataatga tgctgcatta cggcgtacag
     2161 ataagcgcta caaaaccccc agtcactctt gtttttaata tcctcagatc ttttacatga
     2221 ctgataccat tcagatattt taggtaaatt agaaatgttt attgtttttt cgtctggtag
     2281 tgccataggc aaagataata ttgaattata tttcgtttga gcaaaaactt gatttgtcag
     2341 aacaacaaaa aaaattgtgc cagataatac cctttttaat ttaatcatct attccatcca
     2401 caattaaagt tatcattatt cagggcggaa gggtttaacg cttccaatcc ctgcccgtgt
     2461 gctggcggtc tctccctgtc ttctccctga acatctcccg aatccggtta cgttttctca
     2521 gttgctcttc ccggttctgc tcacggaacc gggaagccat tcccagtatc tcgtccatct
     2581 gctcgcgtga aagcccctta aaaggctctg taatgtcctg tacgcgtttc tgctgtattt
     2641 ccatagtctg atgtagctca cgcgctcttt tcgcgttagc ggcgctctgg gacgccacag
     2701 cgacggttcc cgcaaacccg tcgttgac
//
