LOCUS       NZ_CP038403             6675 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli O157:H7 strain BB24-1 plasmid pBB24-2, complete
            sequence.
ACCESSION   NZ_CP038403
VERSION     NZ_CP038403.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05360196
            Assembly: GCF_013168055.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Bono,J.L.
  TITLE     Comparison of complete closed STEC O157:H7 genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Bono,J.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2019) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 166x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 03:24:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,631
            Genes (coding)                    :: 5,309
            CDSs (with protein)               :: 5,309
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 99
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 322
            CDSs (without protein)            :: 322
            Pseudo Genes (ambiguous residues) :: 0 of 322
            Pseudo Genes (frameshifted)       :: 116 of 322
            Pseudo Genes (incomplete)         :: 179 of 322
            Pseudo Genes (internal stop)      :: 79 of 322
            Pseudo Genes (multiple problems)  :: 46 of 322
            Pseudo Genes (short protein)      :: 4 of 322
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP038403.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="BB24-1"
                     /serotype="O157:H7"
                     /isolation_source="cattle feces"
                     /host="cattle"
                     /db_xref="taxon:83334"
                     /plasmid="pBB24-2"
                     /geo_loc_name="USA:Louisiana"
                     /collection_date="2002"
                     /collected_by="USMARC"
     gene            25..2118
                     /gene="cda"
                     /locus_tag="E4U87_RS27965"
                     /old_locus_tag="E4U87_28825"
     CDS             25..2118
                     /gene="cda"
                     /locus_tag="E4U87_RS27965"
                     /old_locus_tag="E4U87_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001286659.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPEGSGVSPL
                     YISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVNQPPVY
                     TQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGVVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDVGGLRDFIYWR
                     PDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            2115..2378
                     /locus_tag="E4U87_RS27970"
                     /old_locus_tag="E4U87_28830"
     CDS             2115..2378
                     /locus_tag="E4U87_RS27970"
                     /old_locus_tag="E4U87_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038407.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNIL
                     NCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            2448..2594
                     /locus_tag="E4U87_RS27975"
                     /old_locus_tag="E4U87_28835"
     CDS             2448..2594
                     /locus_tag="E4U87_RS27975"
                     /old_locus_tag="E4U87_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000726755.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGV
                     AVQ"
                     /name="colicin release lysis protein"
     gene            3301..3489
                     /locus_tag="E4U87_RS27980"
                     /old_locus_tag="E4U87_28850"
     CDS             3301..3489
                     /locus_tag="E4U87_RS27980"
                     /old_locus_tag="E4U87_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024175185.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDEHFLLTYLCG
                     ISHPAWCTFHTILTDAT"
                     /name="hypothetical protein"
     gene            complement(3641..3832)
                     /locus_tag="E4U87_RS27985"
                     /old_locus_tag="E4U87_28855"
     CDS             complement(3641..3832)
                     /locus_tag="E4U87_RS27985"
                     /old_locus_tag="E4U87_28855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3900..4223
                     /locus_tag="E4U87_RS27990"
                     /old_locus_tag="E4U87_28860"
     CDS             3900..4223
                     /locus_tag="E4U87_RS27990"
                     /old_locus_tag="E4U87_28860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4432..>4869
                     /locus_tag="E4U87_RS29100"
                     /pseudo=""
     CDS             4432..>4869
                     /locus_tag="E4U87_RS29100"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4919..5461
                     /locus_tag="E4U87_RS28000"
     CDS             4919..5461
                     /locus_tag="E4U87_RS28000"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000536290.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRDA
                     ISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTALCVTGG
                     IFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSGVKG
                     ENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            5468..5701
                     /locus_tag="E4U87_RS28005"
     CDS             5468..5701
                     /locus_tag="E4U87_RS28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6191..6436)
                     /locus_tag="E4U87_RS28010"
                     /old_locus_tag="E4U87_28875"
     CDS             complement(6191..6436)
                     /locus_tag="E4U87_RS28010"
                     /old_locus_tag="E4U87_28875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000820629.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRARI
                     LKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
                     /name="hypothetical protein"
     BGC             1..6675
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3470..3556
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="99.0"
                     /evalue="1.15e-36"
     Typing          2824..3008
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.774"
                     /coverage="98.0"
                     /evalue="2.18e-82"
     Typing          4214..5808
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.624"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttaaaagtc aaagaggtgt ttttatgagt gattacgaag gtagtggtcc gacagaaggt
       61 attgattatg ggcactcgat ggtcgtgtgg ccgtcaacag gactgatttc gggtggtgat
      121 gtgaaaccag gaggctcatc aggtatcgct ccatccatgc ctccgggatg gggggattac
      181 agcccacagg gtatcgcact tgtacaaagt gttctttttc cgggaattat tcgccggatt
      241 attctggata aggaacttga agagggagac tggtcgggat ggtctgtcag tgtgcatagc
      301 ccttggggaa acgagaaagt ttccgctgca cgaacagttc ttgagaatgg tttacgtggt
      361 ggtttgccag aaccgtctcg cccggctgct gtttcttttg cccgtctgga gcctgcttcc
      421 ggaaatgagc aaaaaattat tcgtcttatg gttacacagc aactggaaca ggtaacggat
      481 attccggcca gccagttacc agcagcgggt aataatgtac cggtgaaata tcgtctgatg
      541 gaccttatgc agaacggtac gcaatatatg gctattatcg gaggtattcc gatgacagtg
      601 ccggtagttg atgccgttcc agttccggac cggagtcgtc cgggaacaaa tattaaagat
      661 gtttacagtg cccctgtatc accaaatcta ccggacctgg tattaagtgt gggtcagatg
      721 aatactccgg ttctgtctaa tcccgaaatt caggaagaag gagttattgc tgagacaggt
      781 aattatgttg aggctggtta tacgatgtcc agtaataatc atgatgtcat tgttcgtttt
      841 cctgaaggca gtggtgtttc tccgttatat atttcaactg tagagattct ggacagtaat
      901 ggtctgagtc agcgtcagga agccgaaaat aaagcaaagg atgatttcag agtcaagaaa
      961 gaagaagcgg tagcccgggc tgaagctgaa aaagcaaaag ccgagttatt tagtaaagca
     1021 ggtgtgaacc agcctcctgt atatacacag gaaatgatgg aaagggctaa ttcagtaatg
     1081 aatgagcagg gagctcttgt tctgaataac actgccagtt ctgtacaact ggcgatgacc
     1141 ggaactggtg tctggactgc tgctggtgat attgcaggca acataagcaa gtttttcagt
     1201 aatgctctgg agaaagtaac catacccgaa gtgagtcccc tgcttatgcg gatttctctt
     1261 ggtgctctgt ggtttcattc agaagaagct ggagcaggaa gtgatatcgt gccggggcga
     1321 aatctggagg cgatgttttc gctgagtgct cagatgttgg ctggacaggg cgtggtcatt
     1381 gaacctggtg cgacgagtgt aaatctgcct gttcgtggac aattgataaa cagtaacggg
     1441 caattagctc tggatttact gaaaacaggg aatgaaagta tcccagctgc ggttcctgtt
     1501 cttaatgctg tccgtgatac agcaacagga ctggataaaa tcacgttacc agcagtagta
     1561 ggcgcacctt cccggacgat tctggttaat ccggtaccac aaccttcggt gccaacagat
     1621 acaggtaatc atcaacccgt tccggttaca ccagtgcata caggaacgga agtaaaaccg
     1681 gtcgaaatgc cagtaacgac gattactcct gtttctgatg ttggtggact acgagatttt
     1741 atttactggc gtcctgatgc tgctgggact ggtgttgaag ctgtttatgt gatgcttaat
     1801 gatcctctgg attcagggcg attttcgcgt aaacaacttg ataaaaaata taaacatgct
     1861 ggtgattttg gtattagtga tacaaaaaag aatcgtgaaa ctcttactaa attcagggat
     1921 gctattgagg agcatttatc ggataaggat acagtagaga aaggaacata ccgaagagaa
     1981 aaaggttcaa aagtttattt taatcctaat acgatgaatg tggttataat taagtcaaat
     2041 ggtgagttct tatctgggtg gaaaataaat ccagatgcgg ataatggtcg aatttattta
     2101 gagacaggtg aactatgaac aagatggcaa tgatcgattt ggcgaaatta tttttagctt
     2161 cgaaaattac agcaattgag ttttcagagc gaatttgtgt tgaacggaga agattgtatg
     2221 gtgttaagga tttgtctccg aatatattaa attgtgggga agagttgttt atggctgctg
     2281 agcgatttga gcctgatgca gatagggctg attatgaaat tgatgataat ggacttaagg
     2341 ttgaggtccg atctatcttg gaaaaattta aattataatc atgagtgatt ttaatatctg
     2401 gcatttaagt ataaatgtca gatattcact catgacaagg agatgtcatg aaaaaaaaga
     2461 tagggggatc tatgattctc gttcttgctg ttttgtgtct gactgcttgc caagctaact
     2521 atgtaaggga tgttcagggt ggaacaattg ctccatcatc atcttcgaaa ctaatcgggg
     2581 tggcggttca gtgaaaaata ttaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2641 ctgctgcctg taaacgaaaa aacccgcctt gtggcgggtc cttccgtccg atcagaagtc
     2701 ttcgacctct tctaatcggc ggtggtaact ggcttggagg agcacagtca ccaaaactgt
     2761 cttttcagtt tagccttaac tggttcataa cttcaagagc aactcctcta aatcagttac
     2821 caatggctgc tgccagtggc gttaagtcgt gtcttttccg ggttggcccc aagagaacag
     2881 ttaccggaga aggtgcagca gtcgggctga acggggggtt cctgcataca gcccagcccg
     2941 gagcgaactg cctaagtcgc cccgagatac cagaagacgt gagctatgag aaagcgccac
     3001 gtgtcccgca agggagaaag gcggaaaagg tatccggtaa tcgatatggc gggaacgtgg
     3061 gggcgcagga gggagcgatt caccggaaac ggtgatgatc tttaagccac gtcgggtttc
     3121 gccaccgctg gcggatttca tggttgagcc tcacggctcc cacagatgca ccgaaaaagt
     3181 gtctgttttg tgaagtccgt caggggggcg gagcctatgg aaaaagcgcc tccggcgcgg
     3241 cctttttcgg ggttttcgtt tcttgctcac atgttctttc cggcgttatc ccctgattct
     3301 gtggataaca ctatcaccgc ttttgagtgg gcagataccg ctcgccgcag ccgaacgacc
     3361 gagcgtagcg agtcagtgag cgaggaagcg gaaaagcgcc tggacgagca ttttctcctt
     3421 acgtatctgt gcggcatttc acacccggca tggtgcactt ttcataccat cctcactgat
     3481 gccacatgat taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     3541 cccgacaccc gctaaaaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     3601 ctcacaggcc agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     3661 ccccgaagcg tgcgaggcag ctgcggtaaa gctcgtcagc gtggtcgtga agcgcttcgc
     3721 agatgtcagc ctggtcgtcg gcgtccagtt cattcagttt ttccagcagg gtcagcgtct
     3781 ggcttctgat gaagcgggcc atgttaaggg cggttttttc ctgtttagtc acagttctgt
     3841 ttctccatgt gtggggagtc tgtcggaggt ttttaatgat accggtgaaa cgcgaaagga
     3901 tgctcacgat acgggtcacg gatgaggaac atgcgcggct gctggcacgt tgtgagggaa
     3961 aacaacttgc gtcatggatg cgtaaggtct gcctgggggc accgccatcg aaaacgtcag
     4021 gtcttcctac actggcaccg ccgctgttgc gccagttcgc ttcggtcggc aataacctga
     4081 atcagatagc ccggaagata aacagtggac agtggtcggg gcatgaccgg gttcatgtgg
     4141 tggctgcact gatggccatc ggacgtgaac tgagtgagct gagggatgag gtccgtaaac
     4201 agggagaacg cgatgatagt taagtttcat gccaggggaa aaggcggtgg cagtggtccg
     4261 gttgattacc tgctgggccg ggagcgtaac cgggaagggg cgagagttct gcggggtgct
     4321 cccgaagagg tgcgggaact catcgatgcc acgccgtttg cgaaaaaata cacctcgggg
     4381 gttctgtctt ttgcagagca gacactgcca ccgggagagc gtgaaagggt gatggagagc
     4441 tttgagcggg tactgatgcc cggtcttgaa aaaaatcagt acagcatcct gtgggtggag
     4501 caccaggaca aggggcgtct tgagctgaat tttgtcatcc cgaatatgga gctggcgagc
     4561 ggaaaacggc tccagcccta ttacgaccgg gcggaccgtc cgcgaatcaa tgcctggcag
     4621 acactggtga atcaccatta cgggctgcac gacccgaacg caccggagaa caggcggaca
     4681 cttgtcaccc cgaataatct gccgaaagcg aaacaggagg ccgcagaagc gattacgagg
     4741 ggactggagg ctctttacca tgccggagcg ctaaaaacgc gtcaggatgt cactgaggcg
     4801 cttacagcgg caggttttga ggtggtgagg acaaccagga gcagtatcag tatcgccgac
     4861 ccggagggcg ggcgtaatct caggctgaaa ggggcgctgt atgagcaatc ttttacaaat
     4921 ggggacagac ttcgagaaga aactgaaaga gcgtgccgtc tctacagaga acatgctgaa
     4981 cagcgagttc aggaagctcg aagcgtctgt agacagggaa ctgaacttaa acgggcagaa
     5041 aatcagagac gctatcagcg cacacacagc agcggtgaaa gaacaactgg agacgctgaa
     5101 cagtgccgtg aacgcgcagt tcagcgccac agagaacgaa ctggcccggc ggcaggaaga
     5161 gctgctctgg aagctggtca aagggcggat actgtacccg tcgctgacgg cgctttgcgt
     5221 cacgggcggg atttttctgg cgtcatgggg gctgattcag tggcaggaaa gccgtattgc
     5281 ggcgaacatc ctggctatca gggaccagga agagacgctg gcgaaactcc gggaaaaaac
     5341 atggggtgtg acgtatcacg agggcaggaa cgggaaattt ctggtgttgc cgtccggggt
     5401 gaagggggaa aacaactgga cggtggagaa gaagaacgca gtaagactgg tcagggagta
     5461 agtgagtatg acagagctgg aaatgcactt actgaacgcc ttagagcaat tgcagcagga
     5521 ctatacgcag cggctgaaag actgggagag cgccttcacg gatttgcagc atatgttcga
     5581 gctgacacga caggagaacg ggcaactgag ggaacagtgc gacgacttga gtcagcaggt
     5641 gcagagatta gccgggcagg tggaccgctt gagtcggtta tttatcacga acagcagata
     5701 agggatgaac aggaaaggca ggcacgcctg gaacgggagc gtgcgcggtc ctggcatgaa
     5761 gagcaggaac gggaagcacg ggtatggcgt ggcccgtcaa tggggatgtg acggtgagtt
     5821 gttccgggag gttgccagat ggtaccggaa cgcgttaaaa acgcatccgg gcgcgattct
     5881 ggcgtgtcgt ttttgttgct gtgttttgcc tctttgccag atcgcgctca ttcctcagcc
     5941 tcatcccgcg atgtctgcgt atgtcatcaa ctgaaaagat gggaaaaaaa cagtgtcatg
     6001 aggtttacgc aggcaaaacc agcgttattc acatggctga atagctatgc atgcggttaa
     6061 aatttatcag gtgcgatcgc ggcagctttt cgggtggttt gttgccattt ttacctgtca
     6121 gcagccgtga tcgcgctgaa cgcatttcag cggtgcgtac aataaggggt tatgttaaat
     6181 aacccttagc ttagcggcgg cgcagtggtt gccaggtgaa ggtgtcatcc gggtcctgtc
     6241 cggcagtcag tgtggcttct cgatttttgc tggccgttcg ttcatcaaga atggctttaa
     6301 gtatccgtgc ccggaggcgt cgctttgttt cctgcggcat tttgtccttt ttcgtcagcc
     6361 ccatggcacg agcttcagcg gcaatggctt ctgccagtcg gcgctcttcg gcttcggtca
     6421 gtctatggcg tttcatggtg gtctccgggt gtttctctgg tgtcataaca gtttaactgt
     6481 tggctctctc cttatgctaa tcagccttct ttcatgcctg ttgccattga gtttaagaat
     6541 gattggttgt ttttcttgag cgttttcaca aaaacgtaaa tccagtgggt tgacaggatt
     6601 tacgaggtga cgtaactttt tactgtacat aaaaccagtg gttttatgta cagtttgttg
     6661 tttttaacgc attgt
//
