LOCUS       NZ_CP038396             6888 bp    DNA     circular CON 14-NOV-2023
DEFINITION  Escherichia coli O55:H7 strain DEC5A plasmid pDEC5A-3, complete
            sequence.
ACCESSION   NZ_CP038396
VERSION     NZ_CP038396.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11229483
            Assembly: GCF_013168015.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O55:H7
  ORGANISM  Escherichia coli O55:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Bono,J.L.
  TITLE     Comparison of complete closed STEC O157:H7 genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Bono,J.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2019) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 275x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/14/2023 02:34:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,293
            CDSs (total)                      :: 5,168
            Genes (coding)                    :: 4,903
            CDSs (with protein)               :: 4,903
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 265
            CDSs (without protein)            :: 265
            Pseudo Genes (ambiguous residues) :: 0 of 265
            Pseudo Genes (frameshifted)       :: 111 of 265
            Pseudo Genes (incomplete)         :: 128 of 265
            Pseudo Genes (internal stop)      :: 71 of 265
            Pseudo Genes (multiple problems)  :: 39 of 265
            Pseudo Genes (short protein)      :: 4 of 265
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP038396.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Escherichia coli O55:H7"
                     /mol_type="genomic DNA"
                     /strain="DEC5A"
                     /serotype="O55:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:244320"
                     /plasmid="pDEC5A-3"
                     /geo_loc_name="USA:New York"
                     /collection_date="1950"
     gene            560..1078
                     /locus_tag="E3155_RS25675"
     CDS             560..1078
                     /locus_tag="E3155_RS25675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001318237.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSC
                     GTLWYLGGMIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPHQ
                     GKDWVQLME"
                     /name="MbeB family mobilization protein"
     gene            1085..1318
                     /locus_tag="E3155_RS25680"
     CDS             1085..1318
                     /locus_tag="E3155_RS25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001555310.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1881..2300)
                     /locus_tag="E3155_RS25685"
                     /old_locus_tag="E3155_26315"
     CDS             complement(1881..2300)
                     /locus_tag="E3155_RS25685"
                     /old_locus_tag="E3155_26315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2381..2818)
                     /locus_tag="E3155_RS25690"
                     /old_locus_tag="E3155_26320"
     CDS             complement(2381..2818)
                     /locus_tag="E3155_RS25690"
                     /old_locus_tag="E3155_26320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001318234.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     gene            3078..4643
                     /locus_tag="E3155_RS25695"
                     /old_locus_tag="E3155_26325"
     CDS             3078..4643
                     /locus_tag="E3155_RS25695"
                     /old_locus_tag="E3155_26325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000447003.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            complement(4640..4981)
                     /locus_tag="E3155_RS25700"
                     /old_locus_tag="E3155_26330"
     CDS             complement(4640..4981)
                     /locus_tag="E3155_RS25700"
                     /old_locus_tag="E3155_26330"
                     /inference="COORDINATES: protein motif:HMM:NF015489.3"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="WP_000058755.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 family microcin immunity protein"
     gene            5029..5166
                     /locus_tag="E3155_RS25705"
                     /old_locus_tag="E3155_26335"
     CDS             5029..5166
                     /locus_tag="E3155_RS25705"
                     /old_locus_tag="E3155_26335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001329514.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(6191..6382)
                     /locus_tag="E3155_RS25710"
                     /old_locus_tag="E3155_26350"
     CDS             complement(6191..6382)
                     /locus_tag="E3155_RS25710"
                     /old_locus_tag="E3155_26350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165988.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDSENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6414..6770
                     /locus_tag="E3155_RS25715"
                     /old_locus_tag="E3155_26355"
     CDS             6414..6770
                     /locus_tag="E3155_RS25715"
                     /old_locus_tag="E3155_26355"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000538666.1"
                     /translation="MGVMIPMKRERMLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGE
                     PVARSGRLPTLAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRR
                     LRLAVREQGARDDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          6019..6106
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(5450..6008)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.606"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..1422
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.229"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6415..6767
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.305"
                     /coverage="100.0"
                     /evalue="1.63e-69"
ORIGIN
        1 atcgatgcca cgccatttgc gaagaaatac acgtccggtg ttctgtcgtt cgcggagaag
       61 gagctgccgc cgggaggacg tgaaaaagtg atggcgagct ttgagcgtgt actgatgccc
      121 ggtctcgaaa aaaatcagta cagcatcctg tgggtggagc accaggacaa gggacggctt
      181 gagctgaatt ttgtcattcc gaacatggag ctggagaccg gaaaacgcct acagccgtac
      241 tacgaccggg cagacaggcc gagaattgat gcctggcaga cgctggtgaa tcaccattac
      301 gggctgcatg acccgaacgc cccggagaac cgccggacgc tgactctccc tgataacctg
      361 ccggaaacga aacaggcgtt tgctgagggc gtcacacgcg ggatagatgc tctttaccat
      421 gcaggagaga taaaaggccg tcaggatgtg attcaggcgc tcacggaagc agggattgaa
      481 gtggtcaggg tgacgcgaag cagtatcagc attgcagacc cgaacggcgg gaagaatatc
      541 agactgaaag gagcattcta tgagcaatct tttacagacg ggcgcggagt tcgagaaaaa
      601 gctgaaagag agagccgaat ctacagagac aatgctgaac gacgagttca gcaagctcga
      661 aaaatctgta agcaaggctg tgacatcaaa cgagacgaaa atcaaagacg ctatagccct
      721 gttcacagct tcgacagagg aatcactgaa aaagcaccgg gaaggggtga aagaggcgat
      781 gatgcagcac aggaaggacg tgttaaagct ggcagggaat acgggcatga tgttactggg
      841 gatggtcatt ttcctgttta ccgtgagttg cgggacgctc tggtatcttg gagggatgat
      901 acaggcgaac ctggaagata tcaggaagca ggaagagaca ttgcagaaac tgaacgcgaa
      961 gacatggggc gtggagttcg tacaggacgg gaacaggaaa tttctggtcc ttccgtacgg
     1021 gaaatcagca gaggtgattc cccatcaggg gaaggactgg gtacaactga tggagtgaca
     1081 cagagtgacg gagctggaaa gacatttgct gaacgcatta gagcaactgc aacaggacta
     1141 tatgcagcgg ctgaacgaat gggagagcgc cttcgtggaa ttgcagaaaa tgttttcgct
     1201 tacgcaacgg gacaacgcga tgctgaacga gcgggtcatg aagttgagtc agcaggtgca
     1261 gcacttgagc gagcggacag aacgcttgag ccagttatac agcgagaaca ggagataagg
     1321 gacgaacggc tgatacagga acgcgaacat gagttatccc tggagcgtga gcgccagctg
     1381 gaaatacagg aacggacact ggatggccct tcgctgggat ggtaatcaga gacaggctaa
     1441 aatgttgtca gcgcgtttcc ggtgcatttt ttgggataat ggttggttcg gggcaccggg
     1501 aacgcgcttc agtcataaac cttgctgaaa acacaggaac gcgttaaaac gcatacgggc
     1561 gcgattctgg tgtgtctttc atagtcgtgt ttttgcctct attgccagat cgcgctcatt
     1621 cccctgaact tctgttttcc tgctcaaatc cccgtcttct tcgcttccga aagtatgaaa
     1681 aaaatccgtt tcagcggtct ctgactggca attcctgcgt gattcacctg gctgcatagg
     1741 tatgcatgtg gttaaaattt accgggtgcg atcgcggcag ttttccgggt ggtttgttgc
     1801 catttttacc tgtctgctgc cgtgatcgca ccgaacgcgt ttcggcggtg cgtacaatta
     1861 agggattatg gtaaatccac ttactgtctg ccctcgtagc catcgagata aaccgcagaa
     1921 atcgtgtcag ccagcagcct ggattttttc ggggtaaggt ctttgctgcg gtaatcagta
     1981 cccgcaaatt gagttgtgcc ccggagtgag tttaaaaatt cctggctgtg aaactgttga
     2041 atgcgttgtt gcacatcact ccgtgatttt cctgtcgctt tatctttagc tcccatatcc
     2101 cagaactgcc gaaaaaaagg caccatcatt cgtgttgtgt cggctttttg ggtggaaaag
     2161 ttgggatcaa aaccgtcatc agaagcatat acgtaatgcc gggtatgttg ttccacgctg
     2221 ccctttgggg tacaggctgt gagagctact gttgctgtta tcatggcgat aagacaaagt
     2281 ttgtttccgg tacgtctcat gagtgtctcc ctaccttaaa gtatttattc tcggtgagtt
     2341 attatccgaa gattatctta tcatcagttt ctgagcgcgt tcagtgaccg aacagcctgg
     2401 accagaatcc ccgtttcttt tcctgctcca gagcctgcct gagctgggct atctcattct
     2461 gtagctgttt ccgttctgct tcctggcgcc ggcgctccgt atcctgggct tgtttatcct
     2521 caagcatcag cgtcaggcat tgtttcagct cattcagttc ccggagaatg cgttctgtct
     2581 gctgatcatg aggtttttct gagtgtcctt gagtgtgcct ttctggtgtc tcattccgct
     2641 ttaattcgcc gtatgcccgg atcagttcac tggtttcaaa ctcccgtcgt ccgtcctggc
     2701 tggtacggta actgacaaga cctgagcgca tatcacggta aaactgtgaa cgcgatcttc
     2761 cagtcatttt cagtgcgtcc cggagtgtgt gccatgccat aaagttacag tgtcccatag
     2821 gtgtctcatc tcatggtttc agtaaaacat aatgaggtct gagaacggta atgtttgtgc
     2881 tggtttttgt ggcatcgggt ggaaatagca cgtggtgtgg gtggctgttt ttttgatcgt
     2941 tttcacaaaa atgaaaatcc acagggttga cagggaaaat gcagcggcgt aattttaaac
     3001 tgtatataaa accagtgatt atatatacag tatattgctt ttaactattt gtttttaatg
     3061 tcaaagagga ttttataatg gaaacagctg tagcgtacta taaagatggt gttccttatg
     3121 atgataaggg gcaggtaatc attactcttt tgaatggtaa tccagacggg agtggctctg
     3181 gcggtggtgg tggaactgga ggtagcaaaa gtgaaagttc tgcagccatt catgccacag
     3241 ctaaatggtc tactgctcaa ttgaagaaaa cgcaggcaga acaggctgcc cgagcaaaag
     3301 ctgccgcaga agcacaggct aaagcaaaag caaaccggga tgcgctgact caacatctga
     3361 aggatattgt gaatgaggcg cttcgccata attccactca tccggaggtt attgacctgg
     3421 ctcatgccaa taatgcagcg atgcaggcag aagcagagcg gttgcgcctt gcaaaagcag
     3481 aagaaaaagc ccgtaaagaa gcggaagctg cggaaaaggc ttttcaggaa gcagaacaac
     3541 gacgtaaaga gatagagaag gagcaggctg aaacagaacg ccaattgaaa ctggctgagg
     3601 atgaagagaa acgcctggca gcattgagtg aagaggcccg ggctgtggag gtggcacaaa
     3661 aaaatcttgc tgctgcacaa tctgagctgg cgaaagtgga tgaagagatt aatacgctca
     3721 atacccgttt aagctccagt attcatgccc gtgatgcaga aacgaatacg ctgtccggaa
     3781 aacgaaatga gttggatcag gcatctgcta aatataaaga actggatgaa agggttaaac
     3841 ttttatctcc tagggcgaat gatccgcttc agagtcgtcc tttttttgag gccaccagac
     3901 tacgggcgag agctggtgat gagatggagg aaaaacaaaa gcaggtaaca gcatcagaaa
     3961 cgcgtcttaa ccagattagc tctgagataa atggaatcca agaggctatt tctcaggcta
     4021 ataataagcg aagtacagca gtttcacgta ttcatgatgc tgaagataat ttgaaaacag
     4081 cacagactaa tctcctgaac tcgcagatta aggatgctgt ggatgcaaca gttagctttt
     4141 atcaaacgct atctgaaaaa tatggtgaaa aatattcaaa aatggcacag gaacttgctg
     4201 ataaatctaa aggtaagaaa atcagcaatg tgaatgaagc tctagctgct tttgagaaat
     4261 acaaggatgt attaaataag aaattcagca aagcagaccg tgatgcaatt ttcaatgcac
     4321 tggaggcggt taagtatgaa gactgggcta agcatttaga tcagtttgcc aagtacttga
     4381 agattacggg acatgtttct tttggatatg atgtggtatc tgatatccta aaaattaagg
     4441 atacaggtga ctggaagccg ctatttctta cattagagaa gaaagctgta gatgctggag
     4501 ttagttatgt tgttgtttta ctttttagtg tgcttgctgg aactacatta ggtatctggg
     4561 ggattgctat tgttacaggc attctatgtg cctttattga taagaataaa cttaatacta
     4621 taaatgaggt gttgggtatt taattattct ttattgatat aaacagtcct agcaatccaa
     4681 atgggattgc taggaccaaa caaagtagat tgtatagcat aaataggttt aattttgcca
     4741 cgggggcatt atttaggttt tttctttttc tgaaaaaatc tttctttatg aacttaaaaa
     4801 atatgtgttc aatagcatat tttgaatatg ggcatagaat agtgcttatc actattgcgt
     4861 atagcatctt atctgacgct aggaaataat acccttcgtt gttttttgtt ataaggtata
     4921 tatatataag tgcgcagtat aggccaaaca aaatattttt tatgtagtat cttaaactca
     4981 taaattaaac ctcgttatat attcttttca ttttataagg atcgagttat gaggaaaaga
     5041 ttttttgtgg gaatattcgc tataaacctc cttgttggat gtcaggctaa ctatatacgt
     5101 gatgttcagg gagggactgt ggctccatca tcttcttcta aattgacggg gatctctgtt
     5161 cagtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgcc
     5221 tgtaaacgaa aaaaccgcct ggggaggcgg tttgatcgaa ggttaagtca gttggggaac
     5281 tgcttaaccg aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttccagtg
     5341 tagccgtagt cgggccacta cttcaagaac tctgttgcac catttgcgcc atcatcgctc
     5401 tgctaaccaa gttaccaacg gctgctgcca gtggcgctaa gtcgtgtctt accgggttgg
     5461 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5521 cacagcccag cttggagcga acgacctaca ccgagccgag ataccaacag cgtgagctat
     5581 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5641 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5701 ctgtcgggtt tcgccacctc tgacttgagc gtcggttttt gtgatgctcg tcaggggggc
     5761 ggagcctatg gaaaaacgcc atcggcgtgg ccttttgttg ggctttgcct ttttgcgcac
     5821 atgttctttc cggtcttatc cccggattct gtggataact gtattatcgt ttttgagtga
     5881 gtcagtaccg ctcgccgcag ccgaacgacc gagcgtagcg agtcagtgag cgaggaagcg
     5941 gaaaagcgcc ggaacgtgca ttttctcctt acgcatctgt gcggtatttc acactggtca
     6001 tggtgcactt ttcatacaat ccgcactgat gctgcatagt taagccagta tacactccgc
     6061 tatcgctacg tgactggttc agggctgcgc cccgaaaccc gctaaaacac cctgacgcgc
     6121 cctggcgggc ttgtctgctc ccggcatccg ctcacaggca agctgtgacc gtctccggga
     6181 gctgcatatg tcagaggttt tcactgtcat ccccgaagcg cgcgaggcag ctgcggtaaa
     6241 gctcatcggc gtggtcgtga agcgattcac agatatctgc ctgttcatcc gcgtccagtt
     6301 cgttgagctt ctccagcagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
     6361 cggttttttc ctgtttagtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
     6421 taatgatacc gatgaaacgc gagaggatgc tcacgatacg ggttactgat gatgaacatg
     6481 cccggttact ggaacgctgt gagggtaaac aactggcggt atggatgcgg cgggtctgcc
     6541 tgggtgagcc ggttgcccgt tccgggaggc tgccgacact ggcaccgccg ttactgcgtc
     6601 agctggcagc catcgggaat aacctgaacc agacagcccg taaggtgaac agcggacagt
     6661 ggtcttccgg cgaccgggtt caggtggtgg ccgcactgat ggccatcggg gatgagctgc
     6721 gccggttgcg tctggccgtc agggaacagg gggcgcggga tgatagttaa gtttcatgcc
     6781 aggggaaaag gtggtggcag tggtccggtt gattacctgc tgggaaggga gcgtaaccgg
     6841 gaaggggcaa cggtgcttca gggtaatccg gaagaagtcc gggaactc
//
