LOCUS       NZ_CP038377             7324 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli O157:H7 strain F1273 plasmid pF1273-2, complete
            sequence.
ACCESSION   NZ_CP038377
VERSION     NZ_CP038377.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11229487
            Assembly: GCF_013167815.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Bono,J.L.
  TITLE     Comparison of complete closed STEC O157:H7 genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7324)
  AUTHORS   Bono,J.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2019) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 433x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 03:26:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,787
            CDSs (total)                      :: 5,647
            Genes (coding)                    :: 5,335
            CDSs (with protein)               :: 5,335
            Genes (RNA)                       :: 140
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 109
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 312
            CDSs (without protein)            :: 312
            Pseudo Genes (ambiguous residues) :: 0 of 312
            Pseudo Genes (frameshifted)       :: 119 of 312
            Pseudo Genes (incomplete)         :: 171 of 312
            Pseudo Genes (internal stop)      :: 73 of 312
            Pseudo Genes (multiple problems)  :: 44 of 312
            Pseudo Genes (short protein)      :: 4 of 312
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP038377.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7324
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="F1273"
                     /serotype="O157:H7"
                     /isolation_source="beef"
                     /db_xref="taxon:83334"
                     /plasmid="pF1273-2"
                     /geo_loc_name="USA"
     gene            24..2117
                     /gene="cda"
                     /locus_tag="E3159_RS27495"
                     /old_locus_tag="E3159_28320"
     CDS             24..2117
                     /gene="cda"
                     /locus_tag="E3159_RS27495"
                     /old_locus_tag="E3159_28320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001282379.1"
                     /translation="MSDNEGSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSSGLAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRSGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPVSQLPAAGN
                     NVPVKYRLTDLMQNGTQYIAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGSGVSPL
                     YISTVEILDRNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVKQPPVY
                     TQEMVERANSVMNEQGTLVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVGPLLMRISLGALWFHSEDAGAGSDIVPGRDMEAMFSLNAQMLAGQGMVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNDSIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPAPQPSAPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDAGGLRDFIYWR
                     PDATGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTNNVVILKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            2114..2377
                     /locus_tag="E3159_RS27500"
                     /old_locus_tag="E3159_28325"
     CDS             2114..2377
                     /locus_tag="E3159_RS27500"
                     /old_locus_tag="E3159_28325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038405.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038406.1"
                     /translation="MNKMAIIDLAKSFLASKITAMEFSERICVERRKLYGVKNLSQDVL
                     NCGEELFIAAERFEPDSDRADYEFDDNGLRIEVQSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            2447..2593
                     /locus_tag="E3159_RS27505"
                     /old_locus_tag="E3159_28330"
     CDS             2447..2593
                     /locus_tag="E3159_RS27505"
                     /old_locus_tag="E3159_28330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071527535.1"
                     /translation="MKKKIGVSIILVLVVLCLTACQNNYVRDVQGGTIAPSSSSKLTGV
                     AIQ"
                     /name="colicin release lysis protein"
     gene            complement(2822..2935)
                     /locus_tag="E3159_RS27510"
     CDS             complement(2822..2935)
                     /locus_tag="E3159_RS27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001428439.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDFTPLAAAIGN"
                     /name="hypothetical protein"
     gene            complement(3605..3796)
                     /locus_tag="E3159_RS27515"
                     /old_locus_tag="E3159_28350"
     CDS             complement(3605..3796)
                     /locus_tag="E3159_RS27515"
                     /old_locus_tag="E3159_28350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165978.1"
                     /translation="MTKQEKTALNMARFIRSQTLILLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3863..4186
                     /locus_tag="E3159_RS27520"
                     /old_locus_tag="E3159_28355"
     CDS             3863..4186
                     /locus_tag="E3159_RS27520"
                     /old_locus_tag="E3159_28355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024177064.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGHWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4395..>4832
                     /locus_tag="E3159_RS29140"
                     /pseudo=""
     CDS             4395..>4832
                     /locus_tag="E3159_RS29140"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4882..5424
                     /locus_tag="E3159_RS27530"
     CDS             4882..5424
                     /locus_tag="E3159_RS27530"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_231200040.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRDA
                     ISAHTAALKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTALCVTGG
                     IFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSGVKG
                     ENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            5431..5664
                     /locus_tag="E3159_RS27535"
     CDS             5431..5664
                     /locus_tag="E3159_RS27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6149..6568)
                     /locus_tag="E3159_RS27540"
                     /old_locus_tag="E3159_28375"
     CDS             complement(6149..6568)
                     /locus_tag="E3159_RS27540"
                     /old_locus_tag="E3159_28375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254598.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(6652..7089)
                     /locus_tag="E3159_RS27545"
                     /old_locus_tag="E3159_28380"
     CDS             complement(6652..7089)
                     /locus_tag="E3159_RS27545"
                     /old_locus_tag="E3159_28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177063.1"
                     /translation="MGHCHFMAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTGQDGRR
                     EFETSELIRAYGELKQDETAERHSAGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
                     /name="hypothetical protein"
     BGC             1..7117
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3433..3520
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          complement(2864..3410)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.011"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4177..5771
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.12"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttttaatgtc aaagaggttt tctatgagtg ataatgaagg tagtgtcccg acagaaggta
       61 ttgattacgg ggacacaatg gttgtgtggc cgtcaactgg acgaattccg ggcggtgatg
      121 tgaaacccgg tggttcatca ggtctcgctc catccatgcc tccgggatgg ggagattaca
      181 gtccacaagg tatcgcactt gtacaaagtg ttctttttcc aggaattatt cgccggatta
      241 tccttgataa ggaacttgaa gagggggact ggtcgggatg gtctgtcagt gtgcatagtc
      301 cctggggaaa cgagaaagtt tctgctgcac gaacagttct tgagaatggt ttacgtagtg
      361 gtttgccaga accatcacgc ccggctgctg tttcttttgc ccgtctggag cctgcttccg
      421 gaaatgagca aaaaattatt cgtcttatgg ttacacagca actggagcag gtaacggata
      481 ttcctgtcag ccagttacca gcagcgggta ataatgtacc ggtgaaatat cgtctgacgg
      541 accttatgca gaacggtacg caatatattg ctattatcgg aggtattccg atgacagtgc
      601 cggtagttga tgccgttccg gttccggacc ggagccgtcc gggaacaaat attaaagatg
      661 tttacagtgc ccctgtatca ccaaatctac cggacctggt attaagtgtg gggcagatga
      721 atactccggt tctgtctaat cccgaaattc aggaagaggg cgttattgct gagacaggta
      781 attatgttga ggcaggttat acgatgtcca gtaataatca tgatgtcatt gttcgttttc
      841 ctgacggcag cggtgtttct ccgttatata tttcaactgt ggagattctt gaccgtaatg
      901 gtctgagtca gcgtcaggaa gcagaaaata aagcaaagga tgatttcaga gtcaagaaag
      961 aagaagcggt agcccgggcc gaagctgaaa aagcaaaagc cgagttattt agtaaagcag
     1021 gtgtgaaaca gcctcctgta tatacacagg aaatggtgga aagggctaat tcagtaatga
     1081 atgagcaggg aactcttgtt ctgaataaca ctgccagttc tgtacaactg gcgatgaccg
     1141 gaactggtgt ctggacggct gctggtgata ttgcaggcaa cataagcaag tttttcagta
     1201 atgctctgga gaaagtaacc atacccgaag tgggtcccct gcttatgcgg atttctcttg
     1261 gcgctctgtg gttccattca gaagacgccg gagcaggaag tgatatcgtg ccggggcgag
     1321 atatggaggc tatgttctcg ctgaatgctc agatgttggc aggacagggc atggtcattg
     1381 aacctggtgc tacgagtgtt aatctgcctg ttcgtggaca attgataaac agtaacggac
     1441 aacttgctct ggatttactg aagacaggga atgatagtat ccctgctgcg gttcctgttc
     1501 ttaatgctgt tcgtgataca gctacaggac tggataaaat tacgctgcca gcagtagtcg
     1561 gtgcaccttc caggacgatt ctggtaaatc cggccccaca accttcggca ccaacagata
     1621 caggaaatca tcaacccgtt ccggttaccc cagtgcatac aggtacggag gtaaaaccgg
     1681 ttgaaatgcc ggtaacgacg attactccag tctctgatgc cggtggactt cgagatttta
     1741 tttactggcg tcctgatgct accgggactg gtgttgaagc tgtctatgtg atgcttaatg
     1801 atcctctgga ttcagggcgg ttttcacgca aacagcttga taaaaaatat aaacatgctg
     1861 gtgattttgg tattagtgat actaaaaaga atcgtgaaac tcttactaaa ttcagggatg
     1921 ctattgagga acatttatcc gataaggata cagtagagaa aggaacatac cgacgagaaa
     1981 aaggatcaaa agtttatttt aatcctaata caaataatgt ggtgatacta aaatcaaatg
     2041 gtgagttctt atctgggtgg aaaataaatc cagatgcgga taatgggcga atttatttag
     2101 aaacaggtga actatgaaca agatggcgat aatcgatttg gcgaagtcat ttttagcttc
     2161 aaaaataaca gcaatggagt tttcagagcg gatttgtgtt gagcggagaa aattgtatgg
     2221 tgttaagaat ttgtctcagg atgtattgaa ttgtggggag gagttgttta tagctgctga
     2281 gcgtttcgag cctgattctg atagggctga ttatgaattc gatgataatg gacttaggat
     2341 tgaagtccaa tctatcctgg aaaaatttaa attataaata tgagtgattt taatatctgg
     2401 aatttaatta taaatgtcag atactcactc atgacaagga gatgttatga aaaaaaagat
     2461 aggggtatcg attattctcg ttcttgttgt tttgtgtctg actgcttgtc aaaataacta
     2521 tgtaagggat gttcagggag gaacgattgc tccatcatca tcctcgaaac taaccggggt
     2581 agcgattcag tgaaaaagat taaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2641 tgctgcttgc aaacaaaaaa accaccgcta ccaacggtgg tttgtttgcc ggaacaagag
     2701 ctaccaactc tttttccgaa ggtaactggc tttagtggag cacagatacc aaatgctgtc
     2761 ctttcagtgt agccgcgata aggcttcaac ttcaagagtt ctgcgtatct aaatccactt
     2821 ttcagttacc aatggctgct gccagtggcg taaagtcgtg tcttaccggg ttggactcaa
     2881 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2941 ccagcttgga gcgaacgacc tacaccgaac tgagatacca acagcgtgag ctatgagaaa
     3001 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaatcggc agggtcggaa
     3061 caggagagcg cacgagggag cttccggggg gaaacgcctg gtatctttaa gtcctgtcgg
     3121 gtttcgccac ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct
     3181 atggaaaaag cgccaccggc gcggcctttt ccccggcttt gtcttgttgc tcacatgttc
     3241 tttccggcgt tatcccctga ttctgtggat aacagtattg ccgcttttgt gtgagcagac
     3301 accgctcgcc gcagccgaac gaccgagcac agcgagtcag tgagcgagga agcggaagag
     3361 catcggcatc tgcatgttct ccttacgcat ctgtgcggca tttcacaccg tacatggtgc
     3421 acttttcata caatcctcac tgatgccaca cgattaagcc agtatacact ccgctatcgc
     3481 tacgtgactg ggtcatggct gcgccccgac acccgctaaa acccgctgac gcgccctgac
     3541 gggcttgtct gctcccggca tccgctcaca ggcaagctgt gaccgtctcc gggagctgca
     3601 tgtgtcagag gttttcaccg tcatccccga agcgtgcgag gcagctgcgg taaagctcgt
     3661 cggcgtggtc gtgaagggct tcgcagatgt ctgcctggtc gtcggcgtcc agttcattca
     3721 gtttttccag caggatcagc gtctggcttc tgataaagcg ggccatgtta agggctgttt
     3781 tttcctgttt agtcacagtt ctgttctcca tgtgtgggga gtctgtcgga ggtttttaat
     3841 gataccggtg aaacgcgaaa ggatgctcac gatacgggtc acggatgagg aacatgcgcg
     3901 gctgctggca cgttgtgagg gaaaacaact tgcgtcatgg atgcgtaagg tctgcctggg
     3961 tgcaccgcca tcgaaaacgt caggtcttcc gacactggca ccgccgctgt tgcgccagtt
     4021 cgcttcggtc ggcaataacc tgaatcagat agcccggaag ataaacagcg gacactggtc
     4081 ggggcatgac cgggttcatg tggtggctgc actgatggcc atcggacgtg aactgagtga
     4141 gctgagggat gaggtccgta aacagggaga acgggatgat agttaagttt catgccaggg
     4201 gaaaaggcgg tggcagtggt ccggttgatt acctgctggg ccgggagcgt aaccgggaag
     4261 gggcgagaat tctgcggggt gatcccgaag aggtgcggga actcatcgat gccacgccgt
     4321 ttgcgaaaaa atacacctcg ggggttctgt cttttgcaga gcagacactg ccaccgggag
     4381 agcgtgaaag ggtgatggag agctttgagc gggtactgat gcccggtttt gaaaaaaatc
     4441 agtacagcat cctgtgggtg gagcaccagg acaaggggcg tcttgagctg aattttgtca
     4501 tcccgaatat ggagctggtg agcggaaaac ggctccagcc ctattacgac cgtgcggacc
     4561 gtccgcgaat caatgcctgg cagacactgg tgaatcatca ttacgggctg cacgacccga
     4621 acgcaccgga gaacaggcgg acacttgtca ccccgaataa tctgccgaaa gcgaaacagg
     4681 aggccgcaga agcgattacg agggggctgg aggctcttta tcatgccgga gcgctaaaaa
     4741 cgcgtcagga tgtcactgag gcgcttacag cggcaggttt tgaggtggtg aggacaacca
     4801 ggagcagtat cagtatcgcc gacccggagg gcgggcgtaa tctcaggctg aaaggggcgc
     4861 tgtatgagca atcttttaca aatggggaca gacttcgaga agaaactgaa agagcgtgcc
     4921 gtctctacag agaacatgct gaacagcgag ttcaggaagc tcgaagcgtc tgtagacagg
     4981 gaactgaact taaacgggca gaaaatcaga gacgctatca gcgcacacac agcagcgctg
     5041 aaagaacaac tggagacgct gaacagtgcc gtgaacgcgc agttcagcgc cacagagaac
     5101 gaactggccc ggcggcagga agagctgctc tggaagctga tcaaagggcg gatactgtac
     5161 ccgtcgctga cggcgctttg cgtcacgggc gggatttttc tggcgtcatg ggggctgatt
     5221 cagtggcagg aaagccggat tgcggcgaac atcctggcta tcagggacca ggaagagacg
     5281 ctggcgaaac tccgggaaaa aacatggggt gtgacgtatc acgagggcag gaacgggaaa
     5341 tttctggtat tgccgtccgg ggtgaagggg gaaaacaact ggacggtgga gaagaagaac
     5401 gcagtaagac tggtcaggga gtaagtgagt atgacagagc tggaaatgca cttactgaac
     5461 gccttagagc aattgcagca ggactatacg cagcggctga aagactggga gagcgccttc
     5521 acggatttgc agcatatgtt cgagctgaca cgacaggaga acgggcaact gagggaacag
     5581 tgcgacgact tgagtcagca ggtgcagaga ttagccgggc aggtggaccg cttgagtcgg
     5641 ttatttatca cgaacagcag ataagggatg aacaggaaag gctggcacgc ctggaacggg
     5701 agcgtgcgcg gtcctggcat gaagagcagg aacgggaagt acgggtatgg cgtggcccgt
     5761 caatggggat gtgacggtga gttgttccgg gaggttgcca gatggtaccg gaacgcgtta
     5821 aaaatgcatc cgggcgcgat tctggcgtgt cgtttttgtt gctgtgtttt gcctctttgc
     5881 cagatcgcgc tcattcctca gcctcatccc gcgatgtctg cgtatgtcat caactgaaaa
     5941 gatggaaaaa aaacagtgtc atgaggttta cgcaggcaaa accagcgtta ttcacatggc
     6001 tgaatagcta tgcatacggt taaaatttat caggtgcgat cgcggcagct tttcgggtgg
     6061 tttgttgcca tttttacctg tcagcagccg tgatcgcgct gaacgaattt cagcggtgcg
     6121 tacaattaag ggattatggt aaatccactt actgtcttcc ctcgtagcca tcgagataaa
     6181 ccgcagaaat cgtctcagcc agcagcctgg attttttcgg ggtaaggtct ttgctgcggt
     6241 aatcagtacc cgcaaattga gttgtgcccc ggagtgagtt taaaaattcc tggctgtgaa
     6301 actgctgaat gcgttgctgc acatcactcc gtgattttcc tgtcgcttta tcctttgctc
     6361 ccatatccca gaactgccgg aaaaaaggca ccatcattcg tgttgtgtcg gccttctggg
     6421 tggaaaagtt aggatcaaaa ccatcgtctg atgcataaac gtaatgccgg gtatgttgtt
     6481 ccacactgcc ctttggcgtg caggctgtca ggaccgttgc cgctgtcagc agggcgataa
     6541 gatacagtgt gccgccggaa cgtctcatga gtgtctcctt acctttaaat ctttgtactc
     6601 agtgacttat atttaaccgc tgactatctt atcatcactt gcagccgatg ctcaccggct
     6661 gaacagtctg gcccagaatc cccgtttttt ttcctgctcc agtgcctgcc tgagctgctc
     6721 tatctcttta tgtagctgtt cccgttcggc ttcctggcgc cggcgttccg tatcctgtgc
     6781 ctgtttgtcc tcaagcatca gtgtcaggca ttgtttcagc tcattcagct cccggagaat
     6841 gcgttctgtc tgctgatcat gtggattttc tgcctgtccc gcactgtgcc tttctgctgt
     6901 ctcatcctgc tttaattcgc cgtatgcccg gatcagttca ctggtttcaa actcccgtcg
     6961 tccgtcctgg ccggtacggt aactgacaag gcctgcgcgc atatcacggt aaaactgtga
     7021 acgcgatctg cctgtcattt tcagtgcgtc ctggagtgtg tgccatgcca tgaagtgaca
     7081 gtgtcccatt ggtgtctcat atcatggttt cagtaaaaca tactggggcc ggagaacggt
     7141 aatgtttgtg ctggtttttg tggcatcggg tgggaatagc acgtggtgtg ggtggctgtt
     7201 tttttgatcg ttttcacaaa aacgaaaatc cacagggttg acagggaaaa cgcagcggcg
     7261 taattttaaa ctgtatataa aaccagtggt tgtatataca gtatgcgtct tttaactatt
     7321 tgtt
//
