LOCUS       NZ_CP038370             2731 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli O157:H7 strain F6321 plasmid pF6321-2, complete
            sequence.
ACCESSION   NZ_CP038370
VERSION     NZ_CP038370.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11229490
            Assembly: GCF_013167755.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Bono,J.L.
  TITLE     Comparison of complete closed STEC O157:H7 genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Bono,J.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2019) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 310x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 03:56:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,652
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,215
            CDSs (with protein)               :: 5,215
            Genes (RNA)                       :: 137
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 300
            CDSs (without protein)            :: 300
            Pseudo Genes (ambiguous residues) :: 0 of 300
            Pseudo Genes (frameshifted)       :: 122 of 300
            Pseudo Genes (incomplete)         :: 162 of 300
            Pseudo Genes (internal stop)      :: 68 of 300
            Pseudo Genes (multiple problems)  :: 44 of 300
            Pseudo Genes (short protein)      :: 4 of 300
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP038370.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="F6321"
                     /serotype="O157:H7"
                     /isolation_source="beef"
                     /db_xref="taxon:83334"
                     /plasmid="pF6321-2"
                     /geo_loc_name="USA"
     gene            join(1950..2731,1..202)
                     /gene="mobV"
                     /locus_tag="E3162_RS27505"
                     /old_locus_tag="E3162_28315"
     CDS             join(1950..2731,1..202)
                     /gene="mobV"
                     /locus_tag="E3162_RS27505"
                     /old_locus_tag="E3162_28315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852935.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_097223437.1"
                     /translation="MANFAIMRCKKLTGFGSVAGALKHCYRERETPNADAGRTPENARF
                     VSETSDQAMGRLREMLPEKRRRDAVLCVEYVMTASPEWWETADQKQQTEFFSRSVQWLT
                     DKYGEGRIIAAVIHRDEETPHLSAFVAPLTQDGRLSAKEFIGGRAKMRDDQTTFAASVR
                     ELGLERGIEGSRATHRTVQHYYESVNRGIRNRVDISPAALEPRVLKKGLFTKVIETPEN
                     VAARLSEAVNDGFAGTVAVASQSAANAKRARELHQTMEIQQKRVQDITEPFKGLSREQM
                     DEILGMASRFREQNREEQLRKRNRIREMFREKTGRDRQHTGRDWKR"
                     /name="mobV"
     gene            249..728
                     /locus_tag="E3162_RS27510"
                     /old_locus_tag="E3162_28320"
     CDS             249..728
                     /locus_tag="E3162_RS27510"
                     /old_locus_tag="E3162_28320"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_097289629.1"
                     /translation="MIKLKRVLSGTIFFVVLTNQVFAQTKYNSILSLPMALPDEKTINI
                     SNLPKISEWYQSCKRSEDIKNKSDWGFCSAYLYAVMQHHYDTSLKKCPNISVQEVLNAV
                     DKEALEYQEMYSKPAKGRDGKLIEGARTYQPNTIYEWNRPAFSVLSSVLEKECIL"
                     /name="hypothetical protein"
     gene            complement(846..1379)
                     /locus_tag="E3162_RS27515"
                     /old_locus_tag="E3162_28325"
     CDS             complement(846..1379)
                     /locus_tag="E3162_RS27515"
                     /old_locus_tag="E3162_28325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279566.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_097294465.1"
                     /translation="MSFSRYKINPFLEDMIVPVKGKQVRLSRLGRDENILVNQSTGEIQ
                     GTHVATFKRVDGEQFVKLFTANIGLTFELSSAGIKAFGVLLWAVQNKALSKDEVDLDSF
                     VLEDFLEAQGATNKQPLRLSLATFKRGINELEKAQIVAKTMRQGRYFINPNFVFNGDRI
                     AFTTVIERQQKIEE"
                     /name="replication/maintenance protein RepL"
ORIGIN
        1 acagcagaaa cgcgtacagg acattacaga gccttttaag gggctttcac gcgagcagat
       61 ggacgagata ctgggaatgg cttcccggtt ccgtgagcag aaccgggaag agcaactgag
      121 aaaacgtaac cggattcggg agatgttcag ggagaagaca gggagagacc gccagcacac
      181 gggcagggat tggaagcgtt aaacccttcc gccctgaata atgataactt taattgtgga
      241 tggaatagat gattaaatta aaaagggtat tatctggcac aatttttttt gttgttctga
      301 caaatcaagt ttttgctcaa acgaaatata attcaatatt atctttgcct atggcactac
      361 cagacgaaaa aacaataaac atttctaatt tacctaaaat atctgaatgg tatcagtcat
      421 gtaaaagatc tgaggatatt aaaaacaaga gtgactgggg gttttgtagc gcttatctgt
      481 acgccgtaat gcagcatcat tatgatacct cactaaaaaa gtgtcctaat atatcagtac
      541 aagaagttct aaatgcagtt gacaaagagg cgttagaata tcaagaaatg tattcaaagc
      601 cagcaaaagg aagagacggt aaattaattg aaggtgcaag aacttatcaa ccaaacacta
      661 tttacgaatg gaatcgacct gcattttcgg tgttatcttc tgttttggaa aaggaatgca
      721 tattgtgatt atttggtcta tacgtaataa acctgaatta tgttaactct gggagtgcct
      781 gtattttaag gcaggcacat agcttgacgc atcaatcaat ctataataga gagttattga
      841 tataattatt cttctatttt ttgttgtcgt tcaatcactg ttgtaaatgc aattcgatct
      901 ccattaaaaa caaaatttgg gttaatgaag tagcggcctt gcctcattgt ttttgcaaca
      961 atctgcgctt tctccaactc gttaataccc cgtttgaatg ttgcaaggct taagcgtagt
     1021 ggttgtttgt ttgtagctcc ctgggcttca agaaagtctt ctaaaacaaa actatccaaa
     1081 tcaacttcgt cttttgaaag cgccttgttc tgaacagccc aaagcaaaac tccaaacgct
     1141 ttaatccccg cagatgatag ctcaaaggtc aaaccgatat ttgctgtgaa gagtttcaca
     1201 aactgttcac catcaactcg cttgaaagtc gctacatgag tgccttggat ctcccccgtt
     1261 gattggttta ctaggatgtt ttcatcacga ccaagacgcg aaagtctaac ttgcttcccc
     1321 ttgactggaa caatcatatc ttctaaaaat gggtttattt tatagcgaga gaagctcatt
     1381 ttaaccacct taaatgatcg acaggattcg attttagata gacggtagct caaaatcaag
     1441 gttttttttg agctaccgta gtactagggt tgagctaccg tagtacagat cctgaaaata
     1501 cgatccccat ttcatgcggg ctgagagtga tccccttctt agttcttaac gaaaaaaatt
     1561 aatcaggcgg ttttctgtca gtctagagcg tacggatttc tatgtccggg gagtggaaat
     1621 tccgcttcct cgatcggaca gactcgcggc gatgccgctc cctaatccgt tcttgcggcg
     1681 agcggttctt gctctttcaa aaacgatatt cattgcctga tttcgtcaag ggcgagcgaa
     1741 gcgagtgcca tttacccttg acgattcacc ggataaaaca ggcttggcag tggtgggagg
     1801 tgacgcggag cgacgccttc cacctgccag accgctgttg aacttgacct ttaggcagct
     1861 tgctgcccga acgattttgc gaagcaaagt ctagtgagta cactatcaat ctgatagtgg
     1921 cccgccggaa cgaggggggg gggggaagca tggcgaactt tgccatcatg cgatgcaaga
     1981 aactgacggg attcggcagt gtggcgggtg cactgaagca ctgctacaga gagcgagaaa
     2041 caccgaatgc cgatgctggc cggacaccgg aaaacgcgcg atttgtctcg gaaacgtcag
     2101 atcaggcgat gggacgactc cgcgaaatgc tgccggaaaa gcgccgcagg gatgcggtgc
     2161 tgtgcgtcga gtacgtgatg acagcaagcc cggagtggtg ggaaacggca gaccagaagc
     2221 agcagaccga atttttttcc cggtctgtgc agtggcttac cgacaagtac ggagagggaa
     2281 ggattatcgc cgccgtgatc caccgtgacg aggaaacgcc gcacctgtcg gcgttcgtcg
     2341 cgcctctcac ccaggatggt cgcctgtcgg caaaggagtt tattggcgga cgtgcgaaga
     2401 tgcgcgatga ccagacgaca tttgcggcgt cagtgaggga gctgggactg gagcgcggga
     2461 ttgaaggaag tcgcgccacg caccggacag tacagcacta ctacgaatct gtgaaccggg
     2521 ggatacgtaa tcgtgtggac atcagccctg ccgcactgga gccgcgcgtg ctgaaaaagg
     2581 gattgttcac gaaagtgatt gagacaccgg agaacgtcgc cgctcgtctg agtgaggcag
     2641 tcaacgacgg gtttgcggga accgtcgctg tggcgtccca gagcgccgct aacgcgaaaa
     2701 gagcgcgtga gctacatcag actatggaaa t
//
