LOCUS       NZ_CP038329             1538 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli O157:H7 strain NE 1092-2 plasmid pNE1092-2,
            complete sequence.
ACCESSION   NZ_CP038329
VERSION     NZ_CP038329.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05360231
            Assembly: GCF_013167455.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Bono,J.L.
  TITLE     Comparison of complete closed STEC O157:H7 genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Bono,J.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2019) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 219x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 03:18:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,881
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,426
            CDSs (with protein)               :: 5,426
            Genes (RNA)                       :: 136
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 118 of 319
            Pseudo Genes (incomplete)         :: 180 of 319
            Pseudo Genes (internal stop)      :: 71 of 319
            Pseudo Genes (multiple problems)  :: 43 of 319
            Pseudo Genes (short protein)      :: 4 of 319
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP038329.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="NE 1092-2"
                     /serotype="O157:H7"
                     /isolation_source="cattle feces"
                     /host="cattle"
                     /db_xref="taxon:83334"
                     /plasmid="pNE1092-2"
                     /geo_loc_name="USA:Nebraska"
                     /collection_date="2000"
                     /collected_by="USMARC"
     gene            join(1519..1538,1..457)
                     /locus_tag="E4U73_RS27955"
                     /old_locus_tag="E4U73_28745"
     CDS             join(1519..1538,1..457)
                     /locus_tag="E4U73_RS27955"
                     /old_locus_tag="E4U73_28745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          1062..1143
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.59"
                     /coverage="100.0"
                     /evalue="2.9e-32"
     Typing          complement(1332..1440)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
     Typing          complement(690..947)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.769"
                     /coverage="99.0"
                     /evalue="1.81e-114"
ORIGIN
        1 acgactgaaa caactttcag atgaggcaaa gaaaaacacc gaggatctcg aagaagcaaa
       61 gaagaattca aggtttacac aggtatcccc aaaaggttgg gaacgcgttc gagagctgct
      121 gaaggatagc caaggcatat cagcactgaa gctgtactca tttttagcgg agcatatcga
      181 tcctacgtgt ggcgctgtcg ttgcggatca gcaattccta gctgaaaaac ttggagttag
      241 cagaagcaca attattcggt ggctcaatta cttagaatca aaaaatgcat tagttagaat
      301 ccccgttgct ggtaaggttt gtgcgtatgc cctcgatcca catgaagtct ggaagggata
      361 caacactacg aaaaaccatg cagcgtttgt cactaaaaca ctggtcaaca aagacggtga
      421 tattcagcgc cgaatcatgg ccatgttttc aaattgagcc agcggcaggc ggacaatcag
      481 gggctacgtg ttaacgtttt gaccatgatt gtctatcctg cattgctctt ttgccgcctc
      541 aaaatccctt gcgtgttttt gctccccgat ccccagaacg accgcgccgc cacggttccg
      601 gctgcgcctt gagcgcaacc ggaaaccgtc tggggagaac cccagacccc catcgctgta
      661 acaccgacgg tctacgacca ccaaccagcc gaatagctgc ctgtgccgtg caaataggct
      721 ttgcagggag ctgacgcggt tcctggcgac ctgaaagccc ctggttaggg gtatcggcgc
      781 gcctgacctc ctgagagcct ctgtaagcgt ttttcgcgcc ttccatgccc tggcgggcat
      841 ttaaacccgt ttcgtgcgtc tgagagcttc tgagcgtgtt tgaagggcta tctgacgact
      901 catccccgaa agaacaacaa cgggatcacc gacaacatgc gagaggtgat cccgctcgcc
      961 gcaagtcgat gaccgagcgt agcgagcgaa tcgacgagga agcggaagag aaccggaagt
     1021 tacattgagc acttacgcac ggatgcgggg tgtcggggtg aagccctgac caagtgggga
     1081 atgtctgagt gcgcgtgcgc ggtcagacat tcccacatcc tgtcccgatt tctgaggcgt
     1141 tttaacggtg aacggacggc aaaaacatga tcgagcgtgg agagcgcgaa atcgggcaaa
     1201 atcacggctg acgaggcttg cagaggagta agccagtata cactccgcta tcgctacgtg
     1261 actgggtcag ggcttcgccc cgacacccca aaagggcgtt gcgttgcgcg caacgccctt
     1321 gcctagaata gatcttttac gccgatttgt aagttgttgt ttttttagtg tgttttttgt
     1381 ttacctgtcg catccagggt gacacaacgt tgtcgcatcc tggatgcgac aacacgcaac
     1441 aaggtgttgc acagatgtgt catgatggct tagatttgaa ccaacgttag tgcatgggat
     1501 tttcagaggg aaaaaatcat gtttattgat tcagaaaa
//
