LOCUS       NZ_CP038325             6673 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli O157:H7 strain NE 1169-1 plasmid pNE1169-2,
            complete sequence.
ACCESSION   NZ_CP038325
VERSION     NZ_CP038325.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05360232
            Assembly: GCF_013167435.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Bono,J.L.
  TITLE     Comparison of complete closed STEC O157:H7 genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   Bono,J.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2019) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 236x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 03:26:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,753
            CDSs (total)                      :: 5,623
            Genes (coding)                    :: 5,323
            CDSs (with protein)               :: 5,323
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 101
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 300
            CDSs (without protein)            :: 300
            Pseudo Genes (ambiguous residues) :: 0 of 300
            Pseudo Genes (frameshifted)       :: 104 of 300
            Pseudo Genes (incomplete)         :: 172 of 300
            Pseudo Genes (internal stop)      :: 72 of 300
            Pseudo Genes (multiple problems)  :: 41 of 300
            Pseudo Genes (short protein)      :: 4 of 300
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP038325.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="NE 1169-1"
                     /serotype="O157:H7"
                     /isolation_source="cattle feces"
                     /host="cattle"
                     /db_xref="taxon:83334"
                     /plasmid="pNE1169-2"
                     /geo_loc_name="USA:Nebraska"
                     /collection_date="2000"
                     /collected_by="USMARC"
     gene            25..2118
                     /gene="cda"
                     /locus_tag="E4U74_RS27130"
                     /old_locus_tag="E4U74_27965"
     CDS             25..2118
                     /gene="cda"
                     /locus_tag="E4U74_RS27130"
                     /old_locus_tag="E4U74_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001286660.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPEGSGVSPL
                     YISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVNQPPVY
                     TQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGVVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDVGGLRDFIYWR
                     PDAAGTGVEAVYVMLNEPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            2115..2378
                     /locus_tag="E4U74_RS27135"
                     /old_locus_tag="E4U74_27970"
     CDS             2115..2378
                     /locus_tag="E4U74_RS27135"
                     /old_locus_tag="E4U74_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038407.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNIL
                     NCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            2448..2594
                     /locus_tag="E4U74_RS27140"
                     /old_locus_tag="E4U74_27975"
     CDS             2448..2594
                     /locus_tag="E4U74_RS27140"
                     /old_locus_tag="E4U74_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000726755.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGV
                     AVQ"
                     /name="colicin release lysis protein"
     gene            complement(3639..3830)
                     /locus_tag="E4U74_RS27145"
                     /old_locus_tag="E4U74_27995"
     CDS             complement(3639..3830)
                     /locus_tag="E4U74_RS27145"
                     /old_locus_tag="E4U74_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165972.1"
                     /translation="MTKQEKTALNMARFIKSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3898..4221
                     /locus_tag="E4U74_RS27150"
                     /old_locus_tag="E4U74_28000"
     CDS             3898..4221
                     /locus_tag="E4U74_RS27150"
                     /old_locus_tag="E4U74_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4430..>4867
                     /locus_tag="E4U74_RS29025"
                     /pseudo=""
     CDS             4430..>4867
                     /locus_tag="E4U74_RS29025"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4917..5459
                     /locus_tag="E4U74_RS27160"
     CDS             4917..5459
                     /locus_tag="E4U74_RS27160"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_100029810.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRDA
                     ISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTALCVTVG
                     IFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSGVKG
                     ENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            5466..5699
                     /locus_tag="E4U74_RS27165"
     CDS             5466..5699
                     /locus_tag="E4U74_RS27165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001345152.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDGLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6189..6434)
                     /locus_tag="E4U74_RS27170"
                     /old_locus_tag="E4U74_28015"
     CDS             complement(6189..6434)
                     /locus_tag="E4U74_RS27170"
                     /old_locus_tag="E4U74_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000820629.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRARI
                     LKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
                     /name="hypothetical protein"
     BGC             1..6673
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3470..3554
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          2824..3008
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.774"
                     /coverage="98.0"
                     /evalue="2.18e-82"
     Typing          4212..5806
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.872"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttaaaagtc aaagaggtgt ttttatgagt gattacgaag gtagtggtcc gacagaaggt
       61 attgattatg ggcactcgat ggtcgtgtgg ccgtcaacag gactgatttc gggtggtgat
      121 gtgaaaccag gaggctcatc aggtatcgct ccatccatgc ctccgggatg gggggattac
      181 agcccacagg gtatcgcact tgtacaaagt gttctttttc cgggaattat tcgccggatt
      241 attctggata aggaacttga agagggagac tggtcgggat ggtctgtcag tgtgcatagc
      301 ccttggggaa acgagaaagt ttccgctgca cgaacagttc ttgagaatgg tttacgtggt
      361 ggtttgccag aaccgtctcg cccggctgct gtttcttttg cccgtctgga gcctgcttcc
      421 ggaaatgagc aaaaaattat tcgtcttatg gttacacagc aactggaaca ggtaacggat
      481 attccggcca gccagttacc agcagcgggt aataatgtac cggtgaaata tcgtctgatg
      541 gaccttatgc agaacggtac gcaatatatg gctattatcg gaggtattcc gatgacagtg
      601 ccggtagttg atgccgttcc agttccggac cggagtcgtc cgggaacaaa tattaaagat
      661 gtttacagtg cccctgtatc accaaatcta ccggacctgg tattaagtgt gggtcagatg
      721 aatactccgg ttctgtctaa tcccgaaatt caggaagaag gagttattgc tgagacaggt
      781 aattatgttg aggctggtta tacgatgtcc agtaataatc atgatgtcat tgttcgtttt
      841 cctgaaggca gtggtgtttc tccgttatat atttcaactg tagagattct ggacagtaat
      901 ggtctgagtc agcgtcagga agccgaaaat aaagcaaagg atgatttcag agtcaagaaa
      961 gaagaagcgg tagcccgggc tgaagctgaa aaagcaaaag ccgagttatt tagtaaagca
     1021 ggtgtgaacc agcctcctgt atatacacag gaaatgatgg aaagggctaa ttcagtaatg
     1081 aatgagcagg gagctcttgt tctgaataac actgccagtt ctgtacaact ggcgatgacc
     1141 ggaactggtg tctggactgc tgctggtgat attgcaggca acataagcaa gtttttcagt
     1201 aatgctctgg agaaagtaac catacccgaa gtgagtcccc tgcttatgcg gatttctctt
     1261 ggtgctctgt ggtttcattc agaagaagct ggagcaggaa gtgatatcgt gccggggcga
     1321 aatctggagg cgatgttttc gctgagtgct cagatgttgg ctggacaggg cgtggtcatt
     1381 gaacctggtg cgacgagtgt aaatctgcct gttcgtggac aattgataaa cagtaacggg
     1441 caattagctc tggatttact gaaaacaggg aatgaaagta tcccagctgc ggttcctgtt
     1501 cttaatgctg tccgtgatac agcaacagga ctggataaaa tcacgttacc agcagtagta
     1561 ggcgcacctt cccggacgat tctggttaat ccggtaccac aaccttcggt gccaacagat
     1621 acaggtaatc atcaacccgt tccggttaca ccagtgcata caggaacgga agtaaaaccg
     1681 gtcgaaatgc cagtaacgac gattactcct gtttctgatg ttggtggact acgagatttt
     1741 atttactggc gtcctgatgc tgctgggact ggtgttgaag ctgtttatgt gatgcttaat
     1801 gaacctctgg attcagggcg attttcgcgt aaacaacttg ataaaaaata taaacatgct
     1861 ggtgattttg gtattagtga tacaaaaaag aatcgtgaaa ctcttactaa attcagggat
     1921 gctattgagg agcatttatc ggataaggat acagtagaga aaggaacata ccgaagagaa
     1981 aaaggttcaa aagtttattt taatcctaat acgatgaatg tggttataat taagtcaaat
     2041 ggtgagttct tatctgggtg gaaaataaat ccagatgcgg ataatggtcg aatttattta
     2101 gagacaggtg aactatgaac aagatggcaa tgatcgattt ggcgaaatta tttttagctt
     2161 cgaaaattac agcaattgag ttttcagagc gaatttgtgt tgaacggaga agattgtatg
     2221 gtgttaagga tttgtctccg aatatattaa attgtgggga agagttgttt atggctgctg
     2281 agcgatttga gcctgatgca gatagggctg attatgaaat tgatgataat ggacttaagg
     2341 ttgaggtccg atctatcttg gaaaaattta aattataatc atgagtgatt ttaatatctg
     2401 gcatttaagt ataaatgtca gatattcact catgacaagg agatgtcatg aaaaaaaaga
     2461 tagggggatc tatgattctc gttcttgctg ttttgtgtct gactgcttgc caagctaact
     2521 atgtaagaga tgttcagggt ggaacaattg ctccatcatc atcttcgaaa ctaatcgggg
     2581 tggcggttca gtgaaaaata ttaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2641 ctgctgcctg taaacgaaaa aacccgcctt gtggcgggtc cttccgtccg atcagaagtc
     2701 ttcgacctct tctaatcggc ggtggtaact ggcttggagg agcacagtca ccaaaactgt
     2761 cttttcagtt tagccttaac tggttcataa cttcaagagc aactcctcta aatcagttac
     2821 caatggctgc tgccagtggc gttaagtcgt gtcttttccg ggttggcccc aagagaacag
     2881 ttaccggaga aggtgcagca gtcgggctga acggggggtt cctgcataca gcccagcccg
     2941 gagcgaactg cctaagtcgc cccgagatac cagaagacgt gagctatgag aaagcgccac
     3001 gtgtcccgca agggagaaag gcggaaaagg tatccggtaa tcgatatggc gggaacgtgg
     3061 gggcgcagga gggagcgatt caccggaaac ggtgatgatc tttaagccac gtcgggtttc
     3121 gccaccgctg gcggatttca tggttgagcc tcatggctcc cacagatgca cagaaaaagc
     3181 gtctgttttg tgaagtccgt caggggggcg gagcctatgg aaaaacgcca gcggcgcgac
     3241 tttttttgcc ctttgccttt ttgctcacat gttctttccg gccttatccc ctgattctgt
     3301 ggataacact atcaccgctt ttgagtgggc agataccgct cgccgcagcc gaacgaccga
     3361 gcgtagcgag tcagtgagcg aggaagcgga aaagcgcctg gacgagcatt ttctccttac
     3421 gcatctgtgc ggcatttcac acccgccatg gcgcactttt catacaattc tcactgatgc
     3481 catatgatta agccagtata cactccgcta tcgctatgtg actgggtcat ggctgcgccc
     3541 cgacacccgc taaaacccgc tgacgcgccc tgacgggctt ctctgctccc ggcatccgct
     3601 cacaggccag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatcc
     3661 ccgaagcgtg cgaggcagct gcggtaaagc tcgtcagcgt ggtcgtgaag cgcttcgcag
     3721 atgtcagcct ggtcgtcggc gtccagttca ttcagttttt ccagcagggt cagcgtctgg
     3781 cttttgatga agcgggccat gttaagggcg gttttttcct gtttagtcac agttctgttt
     3841 ctccatgtgt ggggagtctg tcggaggttt ttaatgatac cggtgaaacg cgaaaggatg
     3901 ctcacgatac gggtcacgga tgaggaacat gcgcggctgc tggcacgttg tgagggaaaa
     3961 caacttgcgt catggatgcg taaggtctgc ctgggggcac cgccatcgaa aacgtcaggt
     4021 cttccgacac tggcaccgcc gctgttgcgc cagttcgctt cggtcggcaa taacctgaat
     4081 cagatagccc ggaagataaa cagcggacag tggtcggggc atgaccgggt tcatgtggtg
     4141 gctgcactga tggccatcgg acgtgaactg agtgagctga gggatgaggt ccgtaaacag
     4201 ggagaacggg atgatagtta agtttcatgc caggggaaaa ggcggtggca gtggtccggt
     4261 tgattacctg ctgggccggg agcgtagccg ggaaggggcg agagttctgc ggggtgctcc
     4321 cgaagaggtg cgggaactca tcgatgccac gccgtttgcg aaaaaataca cctcgggggt
     4381 tctgtctttt gcagagcaga cactgccacc gggagagcgt gaaagggtga tggagagctt
     4441 tgagcgggta ctgatgcccg gtcttgaaaa aaatcagtac agcatcctgt gggtggagca
     4501 ccaggacaag gggcgtcttg agctgaattt tgtcatcccg aatatggagc tggcgagcgg
     4561 aaaacggctc cagccctatt acgaccgggc ggaccgtccg cgaatcaatg cctggcagac
     4621 actggtgaat caccattacg ggctgcacga cccgaacgca ccggagaaca ggcggacact
     4681 tgtcaccccg aataatctgc cgaaagcgaa acaggaggcc gcagaagcga ttacgagggg
     4741 agtggaggct ctttaccatg ccggagcgct aaaaacgcgt caggatgtca ctgaggcgct
     4801 tacagcggca ggttttgagg tggtgaggac aaccaggagc agtatcagta tcgccgaccc
     4861 ggagggcggg cgtaatctca ggctgaaagg ggcgctgtat gagcaatctt ttacaaatgg
     4921 ggacagactt cgagaagaaa ctgaaagagc gtgccgtctc tacagagaac atgctgaaca
     4981 gcgagttcag gaagctcgaa gcgtctgtag acagggaact gaacttaaac gggcagaaaa
     5041 tcagagacgc tatcagcgca cacacagcag cggtgaaaga acaactggag acgctgaaca
     5101 gtgccgtgaa cgcgcagttc agcgccacag agaacgaact tgcccggcgg caggaagagc
     5161 tgctctggaa gctggtcaaa gggcggatac tgtacccgtc gctgacggcg ctttgcgtca
     5221 cggtcgggat ttttctggcg tcatgggggt tgattcagtg gcaggaaagc cgtattgcgg
     5281 cgaacatcct ggctatcagg gaccaggaag agacgctggc gaaactccgg gaaaaaacat
     5341 ggggtgtgac gtatcacgag ggcaggaacg ggaaatttct ggtgttgccg tccggggtga
     5401 agggggaaaa caactggacg gtggagaaga agaacgcagt aagactggtc agggagtaag
     5461 tgagtatgac agagctggaa atgcacttac tgaacgcctt agagcaattg cagcaggact
     5521 atacgcagcg gctgaaagac tgggagagcg ccttcacgga tttgcagcat atgttcgagc
     5581 tgacacgaca ggagaacggg caactgaggg aacagtgcga cggcttgagt cagcaggtgc
     5641 agagattagc cgggcaggtg gaccgcttga gtcggttatt tatcacgaac agcagataag
     5701 ggatgaacag gaaaggctgg cacgcctgga acgggagcgt gcgcggtcct ggcatgaaga
     5761 gcaggaacgg gaagtacggg tatggcgtgg cccgtcaatg gggatgtgac ggtgagttgt
     5821 tccgggaggt tgccagatgg taccggaacg cgttaaaaac gaatccgggc gcgattctgg
     5881 cgtgtcgttt ttgttgctgt gttttgcctc tttgccagat cgcgctcatt cctcagcctc
     5941 atcccgcgat gtctgcgtat gtcatcaact gaaaagatgg aaaaaaaaca gtgtcatgag
     6001 gtttacgcag gcaaaaccag cgttattcac atggctgaat agctatgcat acggttaaaa
     6061 tttatcaggt gcgatcgcgg cagcttttcg ggtggtttgt tgccattttt acctgtcagc
     6121 agccgtgatc gcgctgaacg catttcagcg gtgcgtacaa taaggggtta tgttaaataa
     6181 cccttagctt agcggcggcg cagtggttgc caggtgaagg tgtcatccgg gtcctgtccg
     6241 gcagtcagtg tggcttctcg atttttgctg gccgttcgtt catcaagaat ggctttaagt
     6301 atccgtgccc ggaggcgtcg ctttgtttcc tgcggcattt tgtccttttt cgtcagcccc
     6361 atggcacgag cttcagcggc aatggcttct gccagtcggc gctcttcggc ttcggtcagt
     6421 ctatggcgtt tcatggtggt ctccgggtgt ttctctggtg tcataacagt ttaactgttg
     6481 gctctctcct tatgctaatc agccttcttt catgcctgtt gccattgagt ttaagaatga
     6541 ttggttgttt ttcttgagcg ttttcacaaa aacgtaaatc cagtgggttg acaggattta
     6601 cgaggtgacg taacttttta ctgtacataa aaccagtggt tttatgtaca gtttgttgtt
     6661 tttaacgcat tgt
//
