LOCUS       NZ_CP038297             6211 bp    DNA     circular CON 14-NOV-2023
DEFINITION  Escherichia coli O55:H7 strain TB182A plasmid pTB182A-3, complete
            sequence.
ACCESSION   NZ_CP038297
VERSION     NZ_CP038297.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11229499
            Assembly: GCF_013167255.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O55:H7
  ORGANISM  Escherichia coli O55:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Bono,J.L.
  TITLE     Comparison of complete closed STEC O157:H7 genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Bono,J.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2019) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 441x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/14/2023 02:29:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,268
            CDSs (total)                      :: 5,143
            Genes (coding)                    :: 4,896
            CDSs (with protein)               :: 4,896
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 129 of 247
            Pseudo Genes (internal stop)      :: 70 of 247
            Pseudo Genes (multiple problems)  :: 45 of 247
            Pseudo Genes (short protein)      :: 4 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP038297.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Escherichia coli O55:H7"
                     /mol_type="genomic DNA"
                     /strain="TB182A"
                     /serotype="O55:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:244320"
                     /plasmid="pTB182A-3"
                     /geo_loc_name="USA:Washington"
                     /collection_date="1991"
     gene            complement(322..762)
                     /locus_tag="E3171_RS25105"
                     /old_locus_tag="E3171_25670"
     CDS             complement(322..762)
                     /locus_tag="E3171_RS25105"
                     /old_locus_tag="E3171_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            980..1369
                     /locus_tag="E3171_RS25110"
                     /old_locus_tag="E3171_25675"
     CDS             980..1369
                     /locus_tag="E3171_RS25110"
                     /old_locus_tag="E3171_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            1413..>1817
                     /locus_tag="E3171_RS25115"
                     /old_locus_tag="E3171_25680"
                     /pseudo=""
     CDS             1413..>1817
                     /locus_tag="E3171_RS25115"
                     /old_locus_tag="E3171_25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(1924..2760)
                     /locus_tag="E3171_RS25120"
                     /old_locus_tag="E3171_25685"
     CDS             complement(1924..2760)
                     /locus_tag="E3171_RS25120"
                     /old_locus_tag="E3171_25685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(2760..3563)
                     /gene="aph(3'')-Ib"
                     /locus_tag="E3171_RS25125"
                     /old_locus_tag="E3171_25690"
     ARG             complement(2764..3563)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(3624..4439)
                     /gene="sul2"
                     /locus_tag="E3171_RS25130"
                     /old_locus_tag="E3171_25695"
     ARG             complement(3628..4439)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(4899..5057)
                     /locus_tag="E3171_RS26815"
                     /old_locus_tag="E3171_25705"
     CDS             complement(4899..5057)
                     /locus_tag="E3171_RS26815"
                     /old_locus_tag="E3171_25705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            5628..5777
                     /locus_tag="E3171_RS25135"
     CDS             5628..5777
                     /locus_tag="E3171_RS25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     ARG             complement(1928..2760)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          5420..5593
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(4891..5496)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ccttcctgaa cgtgctgaca gcgggttttc cggctgcgcc ggcaacgctt cacggccacg
       61 caataaattg cgtccccgtt caccgttttc ggatgggaag aacgccggaa acaggactta
      121 ccccggctga atggtgtgaa tttccgcgct atgcacttgt gcgcatactc atgcatgccg
      181 taaaaacaga gcctgcgcgt ttctggcggg ttttcgggtg gtttgttgcc tgttttaccg
      241 gtttcccgtc agaaacgccc tgagggcctc tcaggcggtg ctgataacag atgttatggt
      301 aaatcagagt cacctgcact gttactctga accggagcga aacagccgcg accagaatcc
      361 ccgctttttt tcctgtgcca gcgcgtcagc cagacggcta atttcggcct gtagttgttc
      421 cctctcctca tttcgcaggc ggttttctgc ttcccgcgcc atcttgtctt caagcattag
      481 ggtaacagtc tgctgcagag cgtccagttt ctgctgaatg acgtctagct gagcattttc
      541 cgcctggcta acgacagatt tggagtgtcc ctccttgtgc ctttttggtg tctcagaagt
      601 gcgcagctcg ccataggccc gaatcagttc actggtctcg aactcccggc gatcattttt
      661 tcctgtgcga tagctgacaa ggcctttagc catgtcacga tataattgcg agcgcgactt
      721 tcctgttaat ttcagtgtct cttgaactgt gtgccatgcc ataaaagtga cagtgtctcc
      781 tgtgtgtacc gtattgtaac agcagaacat attactgttt taacacaaaa ggtctgaggc
      841 tgcccgtgag gcgtgaaaaa tacacaaaaa acctgttgac ggtgtggttt tgacgggcgt
      901 agtatttcga atactgtata taaacacatt gttataaata cagtttttgc tgtttttaaa
      961 agtcaaagag gataatttta tgagtacaga gttaaatagc ggtactgtcg gtggttttgg
     1021 tcacatggga ccaaatgacc cctacaatga tggtcatagc ggctctggaa gttcgggagg
     1081 tagttttaac ggtggtggta atggtggcca tgataatagt agtggttcat cttatactgc
     1141 atcctttggt ctcatcactg gccgacaccc gataactgtc ccaggtacga agaatattaa
     1201 ttctgtcgat ctttctgctg tcattaataa cattatacat catgaaggtg cacgcaataa
     1261 catgtaccta gatactgcga acaatgtaag agtgggagtg acgggcactg gctggcaatg
     1321 tctagcaacg gcaggcattt cggctgaggg taaaagaact ttccgctaag cgatagactg
     1381 tatgtaaaca cagtattgca aggacgcgga acatgcctca tgtggcggcc aggacggcca
     1441 gccgggatcg ggatactggt cgttaccaga gccaccgacc cgagcaaacc cttctctatc
     1501 agatcgttga cgagtattac ccggcattcg ctgcgcttat ggcagagcag ggaaaggaat
     1561 tgccgggcta tgtgcaacgg gaatttgaag aatttctcca atgcgggcgg ctggagcatg
     1621 gctttctacg ggttcgctgc gagtcttgcc acgccgagca cctggtcgct ttcagctgta
     1681 agcgtcgcgg tttctgcccg agctgtgggg cgcggcggat ggccgaaagt gccgccttgc
     1741 tggttgatga agtactgcct gaacaaccca tgcgtcagtg ggtgttgagc ttcccgtttc
     1801 agctgcgttt cctgtttggg gtcgtttgcg ggaaggggcg gaatcctacg ctaaggcttt
     1861 ggccagcgat attctccggt gagattgatg tgttcccagg ggataggaga agtcgcttga
     1921 tatctagtat gacgtctgtc gcacctgctt gatcgcggcc gcgatagcta gatcgcgttg
     1981 ctcctcttct ccatccgcgt tccaagctgc ggaaaggcac ccataagcgt acgcctggtc
     2041 gagcaggcga cgcggatcga cgtccagcgc acgagagaat gcgtccgcca tctgtgcaat
     2101 gcgtctagga tcgagacaaa ggtcgtctct gtcagccgga tcgtagaaca tattggcggc
     2161 gccaaagccc acttcaccga ccagaccgac gggatctatc accagccagc cgcgactgga
     2221 gaacatgatg ttttcatgat gcagatcgcc atgtagccca cgcagttccg aggcattgct
     2281 catcatttga tcggctataa tcgccgcgtg gacgtagtca gtttgacaac ctgcgttttg
     2341 atcatcgcgc gcccgctgaa acaaagctgc aaagcgatcc cggatcggga gaagggcaga
     2401 aggcaggggt tcctcagatg cggcatacag cttcgccatt agttccgctg caatttcggt
     2461 cgcctggtag tcgccgtgct cggcaacgat gtgagagagc attcgctccc cggcatattc
     2521 gagcaacatc agattgttct cacgaccgag caaccggact gctcccctcc cattgcgcca
     2581 taccagatag tcggccccgc gcagttcatc agcaatgtct tctataggtt tcaatccctt
     2641 gacgattgca ggagtcccgt ctggcaatga aactttccaa acgaggctgg aaaaggtgtc
     2701 cgcaatgaga acaggttgcg aaacgtgcca atgagcagga aaaacaggcg gcatgaacat
     2761 caaccccaag tcagagggtc caatcgcaga tagaaggcaa ggcgttcgcg gtcgggggct
     2821 tcgatcccca atacattgaa taggacagcg aaggcgcgct ctgcttcatc tggcgctgcc
     2881 cagttctctt cggcgttagc aatcatgagt gccaaatcgg catagcgatc tgctgttccg
     2941 agccgcccaa ggtcgatcag acccgtgcat tgaagagttt tagggtccac catgaagttc
     3001 ggcatgcagg gatcaccatg gcaaacaacc atatcggtgc gctcttggtc gagccgcacc
     3061 ggtagctctc gttcgacacg agccaaaaga tcgagctgcg gcgtactctt gtcctcgtcc
     3121 ggtaagaagt cgggattgac ggcattgcgg gacaccacat caacggcgcg tccgaacatt
     3181 cgcgacagcc tgcgctcaaa cggacattga tcaaccgata ggctgtgaac agcgccaagt
     3241 tgctgcccca ttgacggcca cgctttgagc aaatccgctc cagacagatc agccgccggt
     3301 actcccggaa ttgccgttat caccaagcat gcaccctcct gttcctcctg ccagttgatg
     3361 acctcggggc aagccacacc tcgacctttg agccaaatga ggcggtcacg ctctccagcg
     3421 agctcaccgc ggcgggaagc aggtgcgatt ttcgcgaagg catgcccgtc accacgtcga
     3481 aaaacaaaat caccagattc tccgcctctg acaggcaacc agtcagaatg cgattcacca
     3541 aaaaaaatat tagttcgatt caatggaggt tccttcagtt ttctgatgaa gcgcgaatat
     3601 agagaaatat cccgaatgtg cagttaacga attcttgcgg tttctttcag cgccgccaat
     3661 accgccagcc cgtcgcgcaa ggggcgcggc tcgtgtgtgc ggatgaagtc agctccacct
     3721 gcggcggcgg caagctctgc agcgagtgtc gcggccccga catcccccgg accacggcct
     3781 gtgagcgcgc gcagaaagga tttgcgcgaa acagacagaa gcaccggcaa atcgaagcgc
     3841 agccgcaatt catcgaaccg cgccagcacc gagagcgagg tttcgggagc agcccccaga
     3901 aaaaacccca tgccgggatc aaggacaagg cggttgcgtt tgataccggc acccgtcagc
     3961 gccgcgatgc gcgcgtcaaa gaacgccgca atgtgatcca tgatgtcgcc agcgggtgcc
     4021 tcgcgccgat ctgcctgccc gtcttgcacc gaatgcataa cgacgagttt ggcagatgat
     4081 ttcgccaatt gcggatagaa cgcagcgtct ggaaaaccgc gaatatcatt gagataggcc
     4141 acaccacgcg acaaggcata ggcttgcgtc gcgggttgat aactgtcgag cgagacggga
     4201 atgccatctg ccttgagcgc gtccagcacc ggcgcgatac gcgcgatttc tgtgtcggac
     4261 gaaacaggcg cggcgtcggg attgctggat gccggaccga ggtcgatcac atctgccccc
     4321 tcggccatca gcttacgcgc ctgcgcaatg gctgcgtctg gcgccagata ccggcctcca
     4381 tcggagaaac tgtccgaggt tatgttgacg atgccgaaaa tgatgagcga tttattcatg
     4441 ggggcttcta taataataat aatcgagcat gagtctcata cggatgctcg ggtcgaaagg
     4501 gaatccccag gcgagtaacc tgtttgcggt gatccgtttc atgatgatgt atctgtagca
     4561 ttttggaaat aaatatatca tctctcaata agtagatata tttatctttt ttgcttcttt
     4621 ggtgctgcaa gtcaccaata cgagggaaac ctgttacgga gcggtttaac cgtaatcaac
     4681 agtgactggt gtcatgttcg gaagattaat tgatagtaat ctacaaagga tcttcttgag
     4741 atccttttca atgaatcgta atctcttgct ctgtaaacga aaaaaccgcc tggggaggcg
     4801 gtttgttcga aggttaagtc agttggggaa ctgcttaacc cggtaacagg gttcacgaga
     4861 ccgccattac caaaactgtt ctttcagtgt agccgcagtc aggcctctac ttcaagacct
     4921 cttaatctat cttctcgtgc actcctgtta ctaatggctg ctgccagtgg cgttttgtcg
     4981 tgtctttccg ggttggactc aagttgatag ttaccggaca aggcgcagcg gtcggactga
     5041 acggggggtt cgtgcataca gtccagcttg gagcgaactg ccttcacgga actgagtgtc
     5101 aggcgtggaa tgagaaaccg cggccataac agcggaatga caccggtaaa ccgaaaggca
     5161 ggaacaggag agcgcacgag ggagctacca gggggaaacg cctgggatct ttatagtcct
     5221 gtcgggtttc gccaccactg atttgagcgt cagattttgc gatgcttgtc aggggggcgg
     5281 agcctatgga aaaacggctt cgccgtggcc ttatctcttc tttgctaagt gtcttcctgg
     5341 tatcttccag gaaatttccg tccccgtccg taagccggta ccgctcgccg cagccgaacg
     5401 accgagcgta gcgagtcagt gagcgaggaa gcggaatatc tcctgtatcg catattctgc
     5461 tgacgcgccg gtgctgcctt ttttctcctg ccacatgaag cacttcactg attttcgcaa
     5521 tcgtggcaac atagtaagcc agtatacact ccgctagcgc tacatgactg gttcagggct
     5581 gcgccccgaa acccgctaac aacctctcag ctactggata tcaccccatg ccatctgaat
     5641 atgatgaaca actggattcg actgatacgc agcttcccgg cgaatatgac ctttttgatt
     5701 taatggcgca ggttactgaa gagaacctgc atgcccctgt ggattttggg tcgcctgtcg
     5761 gccgggaagc gttatagctt tttcgcttgc ccgcgtacgt ggtaatactc cggtattacc
     5821 ttttatttct ttattttcat gtaattacag tggattttta atacccgggt attactcgtg
     5881 aaaacgaaat attccttttt tttcttttaa ttcatgcagt tgcgatgaaa atcgcggctg
     5941 cgttaggtgt atgaccattt tgaggttgaa aaataatcaa ccatggcggg tgatttacgc
     6001 aggttaaggg gagatcgtga caaaaaaagc gttcctcact gtatcgttca tgcctgcatg
     6061 aacgggttat tcttcctgac tacagaatta catcgctcct tcagttcata catcacttat
     6121 acatcacttt tacatcactt atacatcact tttacatcat tccttcagct catacgtcac
     6181 tttcatcctc tgtgccattt gtacatcacc c
//
