LOCUS       NZ_CP038296             5423 bp    DNA     circular CON 14-NOV-2023
DEFINITION  Escherichia coli O55:H7 strain TB182A plasmid pTB182A-2, complete
            sequence.
ACCESSION   NZ_CP038296
VERSION     NZ_CP038296.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11229499
            Assembly: GCF_013167255.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O55:H7
  ORGANISM  Escherichia coli O55:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Bono,J.L.
  TITLE     Comparison of complete closed STEC O157:H7 genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Bono,J.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2019) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 441x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/14/2023 02:29:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,268
            CDSs (total)                      :: 5,143
            Genes (coding)                    :: 4,896
            CDSs (with protein)               :: 4,896
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 100 of 247
            Pseudo Genes (incomplete)         :: 129 of 247
            Pseudo Genes (internal stop)      :: 70 of 247
            Pseudo Genes (multiple problems)  :: 45 of 247
            Pseudo Genes (short protein)      :: 4 of 247
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP038296.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Escherichia coli O55:H7"
                     /mol_type="genomic DNA"
                     /strain="TB182A"
                     /serotype="O55:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:244320"
                     /plasmid="pTB182A-2"
                     /geo_loc_name="USA:Washington"
                     /collection_date="1991"
     gene            complement(258..416)
                     /locus_tag="E3171_RS26805"
     CDS             complement(258..416)
                     /locus_tag="E3171_RS26805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(1076..1267)
                     /locus_tag="E3171_RS25065"
                     /old_locus_tag="E3171_25635"
     CDS             complement(1076..1267)
                     /locus_tag="E3171_RS25065"
                     /old_locus_tag="E3171_25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165990.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGGDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1332..1655
                     /locus_tag="E3171_RS25070"
                     /old_locus_tag="E3171_25640"
     CDS             1332..1655
                     /locus_tag="E3171_RS25070"
                     /old_locus_tag="E3171_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955989.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRICLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1864..>2295
                     /locus_tag="E3171_RS26665"
                     /pseudo=""
     CDS             1864..>2295
                     /locus_tag="E3171_RS26665"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2333..2851
                     /locus_tag="E3171_RS25080"
     CDS             2333..2851
                     /locus_tag="E3171_RS25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_139003280.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTTSTEESLKKHREGVKEAMMQHRKDVLKLAGNTGVMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            2858..3091
                     /locus_tag="E3171_RS25085"
     CDS             2858..3091
                     /locus_tag="E3171_RS25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137438.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3122..3283
                     /locus_tag="E3171_RS26810"
                     /old_locus_tag="E3171_25650"
     CDS             3122..3283
                     /locus_tag="E3171_RS26810"
                     /old_locus_tag="E3171_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_071778800.1"
                     /translation="MSYPWSVSASWKYRNAHWMVLHWDGETMKNGRLSGLRRANVVCTI
                     WLHRYSCG"
                     /name="mobilization protein"
     gene            3424..4035
                     /locus_tag="E3171_RS25090"
                     /old_locus_tag="E3171_25655"
     CDS             3424..4035
                     /locus_tag="E3171_RS25090"
                     /old_locus_tag="E3171_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000130481.1"
                     /translation="MTDKTTDIKMALENMIRFRDTIERLGGSMAKEFDVETKGKRTYSM
                     NNDMSDLYNVISRAVEASGADHSINEKLTNVLKRELVDYVSIAHLKTKLSVLYEFEKNY
                     LQLIAEYKEEIKFASSLQEDLRKERAKFFSETLKEVHQTLNESQVDNEVASKWIKELVG
                     SYTKSLDLSGGLVEEHTLDTIACIRAEAKLNKPSIEPGNN"
                     /name="hypothetical protein"
     gene            4038..4577
                     /locus_tag="E3171_RS25095"
                     /old_locus_tag="E3171_25660"
     CDS             4038..4577
                     /locus_tag="E3171_RS25095"
                     /old_locus_tag="E3171_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_171878553.1"
                     /translation="MAGGRHINSLLYVLVAILERKVNDFKLSSSELPEILAALREAREC
                     LVSRSINEDPQLYSTLFLYEKNEGLLSLSFEREYSIFINSNLECILRKVSDIKDKKQGK
                     LQKIMNEFVRFKYSSSDSCADLLFLSGKLEQIRKIYEVFDRDRNRLEFLNYMMKKERVI
                     FEREFERLLGEKNGDF"
                     /name="hypothetical protein"
     gene            4564..5367
                     /locus_tag="E3171_RS25100"
                     /old_locus_tag="E3171_25665"
     CDS             4564..5367
                     /locus_tag="E3171_RS25100"
                     /old_locus_tag="E3171_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000510993.1"
                     /translation="MGIFSQIGSALLNCISKPVDALCEWAKEPLKDREYRREEASKESE
                     HKRNMDVLTAKKRVEHELRMSELNAEYELREKEKTLEVDLEVRRVREIEKAVAEIQEWR
                     KSQELERMERTTAAIARYKEQLTKLNIEVINSIGNMQLDLKERAQKFVYEKAVQYKRLQ
                     DEATEEAMNDLIRIEEKFGDNERAKDILIRAVDTKMGNVIDTSTRFLEELNRDIVSLNQ
                     SIDRLTNQGQKFIESHLERFHIVDATSSLYITGDEKERLLLKNIN"
                     /name="hypothetical protein"
     Typing          914..995
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(345..890)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.255"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1646..3195
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.267"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gttgctaatc attagttttt gtcttgtggt tgaaaactga gcgctatggc tatgacctgg
       61 gagtgggagt gaaaagattc aaggatcctc ttgtgatcct ttttttctgc gcgtaatctg
      121 ctgcctgtaa acaaaaaaac caccgctacc aacggtggtt tgtttgccgg aatctagagc
      181 taccaactct ttcttccgaa ggtaactggc ttggtggagc acagatacca aatactgtcc
      241 ttctagtgta gccgcagtta ggccaccact tcaagaactc ttaatatctc aatccacctt
      301 gtccagttac cagtggctgc tgccagtggc gctttgtcgt gtcttaccgg gttggactca
      361 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
      421 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc aacagcgtga gctatgagaa
      481 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
      541 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
      601 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
      661 ctatggaaaa acgccatcgg cgtggccttt ctcccggcct ttgccttttg cgcacatgtt
      721 ctttccggcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      781 taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga
      841 gcgtcggaag ctgcattttc tccttacgca tctgtgcggc atttcacacc cggcatggcg
      901 cacttttcat acaatctgca ctgatgccgc atggttaagc cagtatacac tccgctatcg
      961 ctacgtgact ggttcagggc ttcgccccga aaccccctga cgcgccctga cgggcttgtc
     1021 tgctcccggc atccgctcac agacaagctg tgaccgtctc cgggagctgt atgtgtcaga
     1081 ggttttcacc gtcacccccg aagcgtgcga ggcagctgcg gtaaagctcg tcggcgtggt
     1141 cgtgaagtga ttcacaaata tcggcctgtt catctgcgtc cagttcgttg agcttctcca
     1201 gcagcgttaa tgtctggctt cttataaagc gggccatgtt aagggcggtt ttttcctgtt
     1261 tagtcactga tgcctccgtg taagggggga tttctgttca tggggtaatg ataccgatga
     1321 aacgcgagag gatgctcacg atacgggtta ctgatgatga acatgcacgg ttactggaac
     1381 gctgtgaggg taaacagctg gcggtatgga tgcggcggat ctgcctgggt gagccggttg
     1441 cccgttccgg gaagctgccg acactggcac cgccgttact gcgccagctg gctgccatcg
     1501 ggaataacct gaatcagacg gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc
     1561 gggttcaggt ggtggccgca ctgatggcca tcgagagaga gttgcgcagc ttacggcagg
     1621 tcgtcaggga acatggagcg cgggatgata gttaagtttc atgccagggg aaaaggcggt
     1681 ggcagtggtc cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg
     1741 cttcagggtg atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa
     1801 tacacgtcag gtgttctgtc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa
     1861 gtgatggcga gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatc
     1921 ctgtgggtgg agcaccagga caagggacgg cttgagctga attttgtcat tccgaatatg
     1981 gagctacaga ccggaaaacg cctccagccg tactacgacc gggcagacag gccgagaatt
     2041 gatgcctggc agacactggt gaatcaccat tacgggctgc atgaccccaa cgccccggag
     2101 aaccgccgga tactgactct ctctgataac ctgccggaaa cgaaacaggc gcttgctgag
     2161 agcgtcacac gcgggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat
     2221 gtgattcagg cgctcacgga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc
     2281 agcattgcag acccgaacgg cgggaagaat atcaggctga aaggagcatt ttatgagcaa
     2341 tcttttacag acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga
     2401 gacaatgctg aacgacgagt tcagcaagct cgaaaaatct gtaagcaagg ctgtgacctc
     2461 aaacgagacg aaaatcaaag acgctatagc cctgttcaca acgtcgacag aggaatcgct
     2521 gaaaaaacac cgggaagggg tgaaagaggc gatgatgcag caccggaagg acgtgttaaa
     2581 gctggcaggg aatacgggcg tgatgttact ggggatagtc tttctcctgt ttaccgcgag
     2641 tggcgggacg ctctggtatc ttggcgggcg gatacaggcg aacctggaag aaatcaggat
     2701 acaggaagag acattgcaga aactgaacgc gaagacatgg ggcgtggagt ttgtgcagga
     2761 tgggagaagg aaatttctgg tcatcccgca ggggaaatcg gcaacggtga ttccctatca
     2821 ggggaaggac tgggtacaac tgacggagtg acacagagtg acggagctgg aaagacattt
     2881 gctgaacgca ttagagcaac tgcaacagga ctatatgcag cggctgaacg aatgggagag
     2941 cgccttcgtg gaattgcaga aaatgttttc gcttacgcaa cgggacaacg cgatgctgaa
     3001 cgagcaggtc atgcagttga gtcagcaggt gcagcacttg agcgagcgga cagaacgctt
     3061 gagccagtta tacagcgaga acaggagata agggacgaac ggctgataca ggaacgcgaa
     3121 catgagttat ccctggagcg tgagcgccag ctggaaatac aggaacgcac actggatggt
     3181 ccttcactgg gatggtgaaa ctatgaaaaa tggcaggtta agtggattaa gacgggctaa
     3241 tgtggtctgt accatctggt tgcataggta ttcatgcggt taaaatttat caggcgcgat
     3301 cgcggcagtt tttcgggtgg tttgttgcca tttttacctg tctgctgccg tgatcgcgct
     3361 gaacgcgttt tagcggtgcg cgtaatgcga cgttatggta aatagatgag ttaggcctaa
     3421 gtcatgacag ataaaacgac cgatattaaa atggctttag aaaacatgat cagatttcgt
     3481 gacactatag agagattggg aggttctatg gcgaaagagt ttgatgttga gaccaaagga
     3541 aaacgtactt attctatgaa taacgatatg agtgatttat ataatgtaat atcacgggct
     3601 gttgaagcgt ctggtgccga tcattcaatt aatgaaaaat tgacaaatgt cttgaaaaga
     3661 gaattagttg attatgtcag cattgcgcat ctaaaaacca aattgtctgt attatatgag
     3721 tttgaaaaga attatttgca gcttatcgca gaatataagg aagaaataaa atttgcttcc
     3781 tctttgcaag aggatttacg taaagaacgt gctaaattct tttctgagac attaaaagag
     3841 gttcatcaaa ctctaaatga atctcaagtt gataatgaag tggcatcaaa atggattaaa
     3901 gaacttgttg gtagttatac caaaagcttg gatctaagcg gaggccttgt tgaagagcat
     3961 acattagata caattgcttg tattcgcgct gaggctaaat taaataaacc atctattgag
     4021 ccgggcaata actaattatg gccgggggta gacatataaa tagtttgctt tatgttcttg
     4081 tggctatatt agaacgtaaa gtgaatgact ttaaattgtc ttcgtcggaa cttcctgaga
     4141 tattggctgc tttacgagag gcaagggaat gccttgtatc gcgcagcatc aatgaagatc
     4201 ctcagttata ttcgacatta tttttatatg aaaaaaatga agggttgcta agtctttcat
     4261 ttgagagaga atactctatt tttattaact ccaatttaga gtgcatattg aggaaagttt
     4321 ctgatataaa agataaaaag cagggtaaac tgcaaaaaat aatgaatgaa tttgtgaggt
     4381 ttaaatattc aagcagcgat tcttgtgcgg atcttttgtt tttgagtggt aaacttgaac
     4441 agatacgtaa aatatatgaa gtctttgata gggatagaaa tagacttgaa tttcttaact
     4501 atatgatgaa gaaagaacgt gttatttttg aaagagagtt tgaacggctt ttaggtgaaa
     4561 aaaatgggga tttttagtca gattggtagc gctttgctta attgtattag taagcctgtt
     4621 gatgcactct gtgaatgggc taaagaacca ttgaaagata gggaatatag aagagaggag
     4681 gcttcaaagg agtctgaaca taagcgcaac atggatgtct tgacggccaa gaaacgtgtt
     4741 gaacatgaac tcagaatgag tgaattgaat gctgaatatg agctgagaga aaaagagaaa
     4801 actcttgaag ttgatctaga ggttcgtcgc gttcgcgaaa tagaaaaagc agtagctgag
     4861 atccaggaat ggagaaagtc acaggaactt gagaggatgg agcgaacaac agcagctatt
     4921 gcacgttaca aggagcaatt aacgaaatta aatatagaag tgattaactc tattgggaac
     4981 atgcagcttg atttaaagga gcgcgcacag aaatttgtct atgagaaagc tgtacaatat
     5041 aagcgcctgc aagatgaagc aactgaagag gctatgaatg atcttataag gattgaggaa
     5101 aagtttggtg ataatgaaag agctaaagat attttaattc gtgcagttga tactaagatg
     5161 gggaatgtta ttgatacgtc aactcgtttt ttggaagaat taaatagaga tattgttagt
     5221 ttaaaccaaa gtattgatag gctgactaat caaggacaaa aattcattga aagtcatttg
     5281 gagcgttttc atattgttga tgcgacatct tctttatata ttacaggtga tgagaaagaa
     5341 cgattgttgc tgaaaaatat taattagtat tttgtgagta atagcgatca ttatttttaa
     5401 tggtcgcatt gtttgttctt gcg
//
