LOCUS       NZ_CP038294             3171 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli O157:H7 strain TB21-1 plasmid pTB21-2, complete
            sequence.
ACCESSION   NZ_CP038294
VERSION     NZ_CP038294.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05360240
            Assembly: GCF_013167235.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Bono,J.L.
  TITLE     Comparison of complete closed STEC O157:H7 genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Bono,J.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2019) Meat Safety and Quality Research Unit,
            United State Department of Agriculture, State Spur 18D, P.O. Box
            166, Clay Center, NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 272x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 02:50:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,619
            CDSs (total)                      :: 5,486
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 103
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 113 of 297
            Pseudo Genes (incomplete)         :: 159 of 297
            Pseudo Genes (internal stop)      :: 76 of 297
            Pseudo Genes (multiple problems)  :: 44 of 297
            Pseudo Genes (short protein)      :: 4 of 297
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP038294.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="TB21-1"
                     /serotype="O157:H7"
                     /isolation_source="cattle feces"
                     /host="cattle"
                     /db_xref="taxon:83334"
                     /plasmid="pTB21-2"
                     /geo_loc_name="USA:Nebraska"
                     /collection_date="2002"
                     /collected_by="USMARC"
     gene            1..324
                     /gene="mobA"
                     /locus_tag="E4U65_RS27790"
                     /old_locus_tag="E4U65_28670"
     CDS             1..324
                     /gene="mobA"
                     /locus_tag="E4U65_RS27790"
                     /old_locus_tag="E4U65_28670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            complement(713..1141)
                     /locus_tag="E4U65_RS27795"
                     /old_locus_tag="E4U65_28675"
     CDS             complement(713..1141)
                     /locus_tag="E4U65_RS27795"
                     /old_locus_tag="E4U65_28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000222531.1"
                     /translation="MTYSVKNKWKAGGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWSLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDRRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKKPVYTVRKTTA"
                     /name="helix-turn-helix domain-containing protein"
     gene            1299..1469
                     /locus_tag="E4U65_RS27800"
     CDS             1299..1469
                     /locus_tag="E4U65_RS27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001531327.1"
                     /translation="MKTEQRHDLVKDIEVFGVSLSLLISRANEKSVTMPSGLSREQRRA
                     WAAEQARKIHN"
                     /name="hypothetical protein"
     gene            1433..1861
                     /locus_tag="E4U65_RS27805"
                     /old_locus_tag="E4U65_28680"
     CDS             1433..1861
                     /locus_tag="E4U65_RS27805"
                     /old_locus_tag="E4U65_28680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000534558.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRYSAKTLDKIDDFIEHFE
                     KYNLFGDPLKGYPAWTGKVSPSWKVPDHYENKEAIEKYARANKLWHAHLGDPVFKDTFH
                     GKYKVSDWVIHFQRLTPNHIKLLELGYHDPMKLPQNFE"
                     /name="hypothetical protein"
     gene            complement(2077..2208)
                     /locus_tag="E4U65_RS27810"
     CDS             complement(2077..2208)
                     /locus_tag="E4U65_RS27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023151671.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLATATGKQDVREM"
                     /name="hypothetical protein"
     gene            complement(2694..2888)
                     /locus_tag="E4U65_RS27815"
                     /old_locus_tag="E4U65_28690"
     CDS             complement(2694..2888)
                     /locus_tag="E4U65_RS27815"
                     /old_locus_tag="E4U65_28690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041620.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2924..3163
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.585"
                     /coverage="100.0"
                     /evalue="3.54e-121"
     Typing          complement(2137..2665)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.393"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          2..321
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="1.97e-62"
ORIGIN
        1 atgacaaaac gaagtggaag taatacgcgc aggcgggcta tcagtcgccc tgttcgtctg
       61 acggcagaag aagaccagga aatcagaaaa agggctgctg aatgcggcaa gaccgtttcc
      121 ggttttttac gggcggcagc tctcggtaag aaagttaact cactgactga tgatcgggta
      181 ctgaaagaag ttatgagact gggggcgtta cagaaaaaac tctttatcga cggcaagcgt
      241 gtcggggaca gggagtatgc ggaggtgctg atcgctatta cggagtatca ccgtgccctg
      301 ttatccaggc ttatggcaga ttagcttccc ggagagaaaa ctgtcgaaaa ctgacggtat
      361 gaacaccgta agctcccaaa gtgatcacca ttcgctttca tgcatagcta tgcagcgagc
      421 tgaaaacgat cctgacgcat ccttcctgtt tttccggggt aaaacatctc tttttgcggt
      481 gtctgcgtca gaatcgcgtt cagcgcgttt cagtggtgcg tacaattaag ggattatggt
      541 aaatatatga gctatgcgat aactttaact gtgaagcgat gaacccatta caggcaaagc
      601 caattactcc tgacagtggt ttagccagaa gcagggctac caagaccaat gcaataagta
      661 atatatcgtt ttgctatcgt gcatcgctcg cgctcagttc cattgtgctt ttttaagctg
      721 tcgtttttct tacggtatat accggttttt tatggcgtgg tttcttaact tgttcagcta
      781 ctgatggacc cattgtatct aggtagtcaa ctagctttgt tagatcataa atattgcgac
      841 caccatatcg gcgatcactc ttcgatgttg gtttcttgtc caagagatta gctttttcaa
      901 gatcattgat agctctctga acagtccgta cagaaacccc catacgtatg gctagacttt
      961 ccattgacgg atgcggccac tcttgcaaac tccaccagtg aacgatcagg ttaagtagtg
     1021 tgttaaaggc cactgaagtt agctttttct cgttttgtat aaaaaacaat acggtaggca
     1081 ctgctgtcca gccaagagac aaaccgccag ctttccattt attcttaacg gagtaagtca
     1141 ttgattttcc taagccccaa aatatttaaa gtatatatta tatggtatat tcatatgaat
     1201 agggtgacac tggcgccatt attgtgcaac caaaaaagac tactctgaaa acgaggaaaa
     1261 gattttttcc tgcctgaatt agatacggag ttagcgatat gaaaaccgaa caacgtcatg
     1321 atcttgttaa agatattgag gtttttggcg tatccttgtc tctgttgatt tccagagcga
     1381 atgagaagtc tgttacaatg ccatctggtc taagtcggga gcagagaaga gcatgggcag
     1441 cggagcaggc gcgcaaaatc cacaattgaa tattgtctca ttctctgaga ccttcaacct
     1501 ttattacaca tccagatatt ctgcaaaaac actcgataaa atcgatgatt tcattgagca
     1561 ttttgaaaaa tacaatctct ttggcgatcc tttaaaagga tatccagctt ggactggcaa
     1621 agtatcgcca tcgtggaaag tgcctgatca ttacgaaaac aaagaagcta ttgagaagta
     1681 tgctagagct aacaaattat ggcatgctca tttaggcgat ccggttttta aagatacgtt
     1741 tcatgggaaa tacaaggttt ctgactgggt tattcatttc cagcggctga caccgaacca
     1801 tataaagctt ttagagcttg gataccatga cccaatgaag ctacctcaaa actttgaatg
     1861 atcgagcggc aggctaaatg aaatcttgag attcattcag tctcgtcgta atctcactat
     1921 tgtaaaaacg aaaaaaccac cctggcaggt ggtttttcga aggttagtta atcctggcag
     1981 attctctaac cgtggtaaca gtcttgtgcg agacatgtca ccaaatactg tcctttcagt
     2041 gtagcctcag ttaggccgcc acttcaagaa ctctcgttac atctctcgca catcctgctt
     2101 accagtggcc gttgccagtg gcgttaagtc gtgtcttacc gggttggact caagacgata
     2161 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2221 ggagcgaacg acctacaccg aaccgagata ccaacagcgt gagctatgag aaagcgccac
     2281 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2341 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2401 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2461 aaacgccaac ggcgccgcct tttccgggtt ctcgtttttt gctcacatgt ttcttttggc
     2521 gttatcccct gattctgtgg ataaccgcat ctccgctttt gagtgagccg acaccgctcg
     2581 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaag agcgccttat
     2641 gtgacatttt ctccttacgc tctgttgtgc cgttcggcat cctgtcctga gagttatctc
     2701 tctgtgctat tttctacttc aaagcgtgtc tggatgctgt tctggagttc ttctgcgagt
     2761 tcgtgcagct tctcacacat ggcagcctgt tcgtcagcat cgagtgcgtc cagcttttcg
     2821 agcagcgtca ggctctggct ttttatgaat cctgccatgt tgagtacggc ttgctgctgc
     2881 ttgttcatct tttcgttttc tccgttctgt ctgtcatctg cgttgtgtga ttatatcgcg
     2941 taccactttt cgactgtttt accgccggta ttctgccgtc tgacgctttg acaggtattt
     3001 ctgcctgaca atactgtcac tgccaaaaaa ctgccgtgcc tttgtcggta attcgaactt
     3061 gctgacagga caggatgtgt aattgttata ccgcgcatac atgcacgcta ttacaattac
     3121 cctggtcagg gctttgcccc gacacccatg tcagatacgg agccatgttt t
//
