LOCUS       NZ_CP038181            33309 bp    DNA     circular CON 18-JUN-2024
DEFINITION  Escherichia coli strain 2 HS-C plasmid p2HS-C-1, complete sequence.
ACCESSION   NZ_CP038181
VERSION     NZ_CP038181.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10986381
            Assembly: GCF_004526255.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33309)
  AUTHORS   Buedel,T., Clement,M., Kuenzli,E., Bernasconi,O.J., Fehr,J.,
            Mohammed,A.H., Hassan,N.K., Zinsstag,J., Hatz,C. and Endimiani,A.
  TITLE     Polyclonal Gut Colonization with Extended-Spectrum Cephalosporin-
            and Colistin-Resistant Enterobacteriaceae: An Ordinary Status for
            Hotel Employees in Zanzibar Island, Tanzania
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 33309)
  AUTHORS   Clement,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2019) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern, Bern 3001,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2019
            Assembly Method                   :: Canu v. 1.7.1; Pilon v. 1.22
            Assembly Name                     :: 2HS-C
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004526255.1-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 06:20:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,598
            CDSs (total)                      :: 4,480
            Genes (coding)                    :: 4,218
            CDSs (with protein)               :: 4,218
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 75 of 262
            Pseudo Genes (incomplete)         :: 186 of 262
            Pseudo Genes (internal stop)      :: 48 of 262
            Pseudo Genes (multiple problems)  :: 43 of 262
            Pseudo Genes (short protein)      :: 1 of 262
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP038181.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..33309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2 HS-C"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2HS-C-1"
                     /geo_loc_name="Tanzania"
                     /collection_date="2018-06"
     gene            1..2748
                     /locus_tag="E4F32_RS22070"
                     /old_locus_tag="E4F32_22070"
     CDS             1..2748
                     /locus_tag="E4F32_RS22070"
                     /old_locus_tag="E4F32_22070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_000105985.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            2760..3476
                     /locus_tag="E4F32_RS22075"
                     /old_locus_tag="E4F32_22075"
     CDS             2760..3476
                     /locus_tag="E4F32_RS22075"
                     /old_locus_tag="E4F32_22075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000832283.1"
                     /translation="MKRVKTLMLISLLTASFYSSAGIPVAIDASPEWAVEAARWTERLK
                     QWSETAQHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLSSAM
                     SQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLVTQEQ
                     EAFIKQQKEAEPLTFD"
                     /name="type IV secretion system protein"
     gene            3488..4582
                     /locus_tag="E4F32_RS22080"
                     /old_locus_tag="E4F32_22080"
     CDS             3488..4582
                     /locus_tag="E4F32_RS22080"
                     /old_locus_tag="E4F32_22080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677777.1"
                     /GO_process="GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000046889.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     IRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAA
                     GMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALA
                     PTGALGMKARRLASLEKARARNAA"
                     /name="type IV secretion system protein"
     gene            5097..5783
                     /locus_tag="E4F32_RS22100"
                     /old_locus_tag="E4F32_22100"
     CDS             5097..5783
                     /locus_tag="E4F32_RS22100"
                     /old_locus_tag="E4F32_22100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001290765.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTSYQTD
                     REVRGE"
                     /name="type IV secretion system protein"
     gene            5780..6688
                     /gene="virB9"
                     /locus_tag="E4F32_RS22105"
                     /old_locus_tag="E4F32_22105"
     CDS             5780..6688
                     /gene="virB9"
                     /locus_tag="E4F32_RS22105"
                     /old_locus_tag="E4F32_22105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_134778716.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQELEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWNYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="virB9"
     gene            6681..7799
                     /gene="virB10"
                     /locus_tag="E4F32_RS22110"
                     /old_locus_tag="E4F32_22110"
     CDS             6681..7799
                     /gene="virB10"
                     /locus_tag="E4F32_RS22110"
                     /old_locus_tag="E4F32_22110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_000131271.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSQTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHT
                     KLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGW
                     IDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSIN
                     IPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="virB10"
     gene            7801..8829
                     /gene="virB11"
                     /locus_tag="E4F32_RS22115"
                     /old_locus_tag="E4F32_22115"
     CDS             7801..8829
                     /gene="virB11"
                     /locus_tag="E4F32_RS22115"
                     /old_locus_tag="E4F32_22115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000017226.1"
                     /translation="MSGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDSVTFDFCKRFSHTLASFRGDEIGETKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="virB11"
     gene            8816..10678
                     /gene="taxB"
                     /locus_tag="E4F32_RS22120"
                     /old_locus_tag="E4F32_22120"
     CDS             8816..10678
                     /gene="taxB"
                     /locus_tag="E4F32_RS22120"
                     /old_locus_tag="E4F32_22120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxB"
                     /protein_id="WP_170324155.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKENIF
                     GDARFATINDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSSVTHRFNPLYYVSMG
                     TSEGFNQLENLSLIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLNEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLVVKDVPDLDKAA"
                     /name="taxB"
     gene            10687..11109
                     /locus_tag="E4F32_RS22125"
                     /old_locus_tag="E4F32_22125"
     CDS             10687..11109
                     /locus_tag="E4F32_RS22125"
                     /old_locus_tag="E4F32_22125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_015059903.1"
                     /translation="MMKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFH
                     GVIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKME
                     GATAVVEWRNEKSFLPEQVKIVFIKAQNEVKNDWKK"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            11093..11398
                     /locus_tag="E4F32_RS22130"
                     /old_locus_tag="E4F32_22130"
     CDS             11093..11398
                     /locus_tag="E4F32_RS22130"
                     /old_locus_tag="E4F32_22130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_001350475.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            <11511..11705
                     /locus_tag="E4F32_RS23180"
                     /pseudo=""
     CDS             <11511..11705
                     /locus_tag="E4F32_RS23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /name="ATP-binding protein"
     gene            11709..12281
                     /locus_tag="E4F32_RS22135"
                     /old_locus_tag="E4F32_22135"
     CDS             11709..12281
                     /locus_tag="E4F32_RS22135"
                     /old_locus_tag="E4F32_22135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001198960.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPFH
                     ICYGGYLLNPGVSDSDISRKIKDQAGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERRFRV
                     VSKDNWQHTVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     gene            12286..14439
                     /locus_tag="E4F32_RS22140"
                     /old_locus_tag="E4F32_22140"
     CDS             12286..14439
                     /locus_tag="E4F32_RS22140"
                     /old_locus_tag="E4F32_22140"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="WP_001235745.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQAAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPARAKK"
                     /name="type IA DNA topoisomerase"
     gene            14436..14642
                     /gene="hha"
                     /locus_tag="E4F32_RS22145"
                     /old_locus_tag="E4F32_22145"
     CDS             14436..14642
                     /gene="hha"
                     /locus_tag="E4F32_RS22145"
                     /old_locus_tag="E4F32_22145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     gene            14660..15124
                     /locus_tag="E4F32_RS22150"
                     /old_locus_tag="E4F32_22150"
     CDS             14660..15124
                     /locus_tag="E4F32_RS22150"
                     /old_locus_tag="E4F32_22150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0030527 - structural constituent of chromatin [Evidence
                     IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293134.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIEKTKPEQAE"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            15211..15915
                     /locus_tag="E4F32_RS22155"
                     /old_locus_tag="E4F32_22155"
     CDS             15211..15915
                     /locus_tag="E4F32_RS22155"
                     /old_locus_tag="E4F32_22155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            16698..16793
                     /gene="dqlB"
                     /locus_tag="E4F32_RS23760"
     CDS             16698..16793
                     /gene="dqlB"
                     /locus_tag="E4F32_RS23760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_021534057.1"
                     /translation="MINMLIIVLRAVVAVANALIAVLELIRQFID"
                     /name="dqlB"
     gene            17456..18103
                     /locus_tag="E4F32_RS22175"
                     /old_locus_tag="E4F32_22175"
     CDS             17456..18103
                     /locus_tag="E4F32_RS22175"
                     /old_locus_tag="E4F32_22175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000587671.1"
                     /translation="MIIVIGGDKGGTGKSHLATNLTVCLSQQGKRTGLVETDLNGSTKK
                     WNKRREQAGLPPVALNEAYGDISAKITKMADVAEILIIDTAGYDSTEFRTALKVADIVI
                     VPIDPLAQVEADSLQTVTKIVRDAQKINPRLAAHVLLYKCQPNTFSEQQELRDSLNSHD
                     YWLKPMKSTVSFLRAFVRAMNQGMGVHELKSNVSGASQAKAQIELLLKELEL"
                     /name="division plane positioning ATPase MipZ"
     gene            18178..18447
                     /locus_tag="E4F32_RS22180"
                     /old_locus_tag="E4F32_22180"
     CDS             18178..18447
                     /locus_tag="E4F32_RS22180"
                     /old_locus_tag="E4F32_22180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001086090.1"
                     /translation="MAKLELPELEQNQEVNATKFVQDAGKRPTENKTKLASFRIPNELL
                     EFIDNESKRLHRPKTKIIKAALLAYQDLDENELINLWFRADRND"
                     /name="hypothetical protein"
     gene            18608..19237
                     /locus_tag="E4F32_RS22190"
                     /old_locus_tag="E4F32_22190"
     CDS             18608..19237
                     /locus_tag="E4F32_RS22190"
                     /old_locus_tag="E4F32_22190"
                     /inference="COORDINATES: protein motif:HMM:NF022440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2806 domain-containing protein"
                     /protein_id="WP_223424520.1"
                     /translation="MSLIDLSLSGLSEPGTKLIEKISDAIGVLYEPTRIRKKAKAEAEA
                     KRTELISRLELEGIEKRAVERFLKRETKRQENIENITMQAAQSLSESDNVSDIDEDWIE
                     AFFRECEDISDEQMQMLWGRILSEEAKSKGSFSRRTLKLLSTISKEEANLITYFGKFVW
                     QANKLTPILFTDENGDTEGITFDKLSVLDSLGVIQQGIGYSLRYKL"
                     /name="DUF2806 domain-containing protein"
     gene            19411..21036
                     /locus_tag="E4F32_RS22195"
                     /old_locus_tag="E4F32_22195"
     CDS             19411..21036
                     /locus_tag="E4F32_RS22195"
                     /old_locus_tag="E4F32_22195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_049589868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-1.1"
                     /protein_id="WP_049589868.1"
                     /translation="MMQHTSVWYRRSVSPFVLVASVAVFLTATANLTFFDKISQTYPIA
                     DNLGFVLTIAVVLFGAMLLITTLLSSYRYVLKPVLILLLIMGAVTSYFTDTYGTVYDTT
                     MLQNALQTDQAETKDLLNAAFIMRIIGLGVLPSLLVAFVKVDYPTWGKGLMRRLGLIVA
                     SLALILLPVVAFSSHYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYH
                     AKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSC
                     GTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLP
                     KAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAY
                     FKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDV
                     SMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDA
                     ITPTLLKLFDVTADKVKDRTAFIR"
                     /name="phosphoethanolamine--lipid A transferase MCR-1.1"
     gene            21108..21830
                     /locus_tag="E4F32_RS22200"
                     /old_locus_tag="E4F32_22200"
     CDS             21108..21830
                     /locus_tag="E4F32_RS22200"
                     /old_locus_tag="E4F32_22200"
                     /inference="COORDINATES: protein motif:HMM:NF028536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family protein"
                     /protein_id="WP_039003037.1"
                     /translation="MPYLSNKRLLAEMSIALVMAIVATLTLEHSQIDLMVADWFYLGMG
                     HWMVAKQAFLPDLLLYSGLKKLLMAMLIYLLVATICRAYHEKKGNAITAKWLVPVTKFR
                     VRELAYLVLTLILVPTVVASLKAYTHVVCPVHLTIFDGTLPYLPMLDSMRNTIPDKCFP
                     AAHASSGFALFAFAFAPSLRRRRGAIIIVVMALGWAMGCYKMIIGDHFLSHTVVSMMLA
                     WAMSAGLAWVFFKKGEQV"
                     /name="PAP2 family protein"
     gene            complement(22221..22748)
                     /locus_tag="E4F32_RS22210"
                     /old_locus_tag="E4F32_22210"
     CDS             complement(22221..22748)
                     /locus_tag="E4F32_RS22210"
                     /old_locus_tag="E4F32_22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_000577043.1"
                     /translation="MIFNRNKKRDEQHNSAVVLPSAENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNNESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="DUF2726 domain-containing protein"
     gene            complement(22883..23287)
                     /locus_tag="E4F32_RS22215"
                     /old_locus_tag="E4F32_22215"
     CDS             complement(22883..23287)
                     /locus_tag="E4F32_RS22215"
                     /old_locus_tag="E4F32_22215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012421203.1"
                     /translation="MKDNIVDFQRIRDSMNDHSTDVANDAVYSDKKKISRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSSYVVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     gene            complement(23300..24166)
                     /locus_tag="E4F32_RS22220"
                     /old_locus_tag="E4F32_22220"
     CDS             complement(23300..24166)
                     /locus_tag="E4F32_RS22220"
                     /old_locus_tag="E4F32_22220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473366.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001353743.1"
                     /translation="MTTNKTSLSRLTKVRHRNELNSTLSTLPMAAKKVLFLAMCQINSK
                     NEFDDDHIFYVTVADYIKWVQVKPDAAYLALRDGSNILDTTLLKLKHDEILELSSDLGF
                     KFTKSNVPDSMNLSLTVFSTYYRNEGRVGIKFTKEAKRFLCKLIGEEKRYTTQVLLSVV
                     RLTSVNAASLYQLIRKIYSNNSRANSFEMTIDELKDELNLYTIGAGGVKDYKYPDYPAF
                     KRDVLNKSVKEIMKHTEVKNLSFVVSEKIGRKVYKLKFSYTIGYEGDTREDSEFTNMFD
                     KMYPPEN"
                     /name="replication initiation protein"
     gene            25581..26096
                     /locus_tag="E4F32_RS22225"
                     /old_locus_tag="E4F32_22225"
     CDS             25581..26096
                     /locus_tag="E4F32_RS22225"
                     /old_locus_tag="E4F32_22225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025395.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="J domain-containing protein"
     gene            26201..26743
                     /locus_tag="E4F32_RS22230"
                     /old_locus_tag="E4F32_22230"
     CDS             26201..26743
                     /locus_tag="E4F32_RS22230"
                     /old_locus_tag="E4F32_22230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083906.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            26740..26991
                     /locus_tag="E4F32_RS22235"
                     /old_locus_tag="E4F32_22235"
     CDS             26740..26991
                     /locus_tag="E4F32_RS22235"
                     /old_locus_tag="E4F32_22235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230707.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            26978..27205
                     /locus_tag="E4F32_RS22240"
                     /old_locus_tag="E4F32_22240"
     CDS             26978..27205
                     /locus_tag="E4F32_RS22240"
                     /old_locus_tag="E4F32_22240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000801440.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     gene            27659..27958
                     /locus_tag="E4F32_RS22250"
                     /old_locus_tag="E4F32_22250"
     CDS             27659..27958
                     /locus_tag="E4F32_RS22250"
                     /old_locus_tag="E4F32_22250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000650309.1"
                     /translation="MKCSSVFTSTTNHVFTFERVTLCTIILMHKDTGQQYVVIFTDNNK
                     IRDYKAGIVPQFGELKQSDVDLVLFYRDEYEKYFDSLKDGDECLSFKDFIECLC"
                     /name="hypothetical protein"
     gene            28049..28399
                     /locus_tag="E4F32_RS22255"
                     /old_locus_tag="E4F32_22255"
     CDS             28049..28399
                     /locus_tag="E4F32_RS22255"
                     /old_locus_tag="E4F32_22255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000854259.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGKGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGND"
                     /name="hypothetical protein"
     gene            28704..28952
                     /locus_tag="E4F32_RS22260"
                     /old_locus_tag="E4F32_22260"
     CDS             28704..28952
                     /locus_tag="E4F32_RS22260"
                     /old_locus_tag="E4F32_22260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653200.1"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family toxin"
                     /protein_id="WP_000520549.1"
                     /translation="MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFF
                     NAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGLL"
                     /name="type II toxin-antitoxin system HicA family toxin"
     gene            28949..29296
                     /locus_tag="E4F32_RS22265"
                     /old_locus_tag="E4F32_22265"
     CDS             28949..29296
                     /locus_tag="E4F32_RS22265"
                     /old_locus_tag="E4F32_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="WP_000681613.1"
                     /translation="MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQ
                     EFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNKAL
                     EKEVSNHRAGA"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            29657..30160
                     /gene="taxA"
                     /locus_tag="E4F32_RS22275"
                     /old_locus_tag="E4F32_22275"
     CDS             29657..30160
                     /gene="taxA"
                     /locus_tag="E4F32_RS22275"
                     /old_locus_tag="E4F32_22275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxA"
                     /protein_id="WP_000781813.1"
                     /translation="MKMIAFRLSDDELKFAEHNAILSGFTSINAFAKHNVLNIETKPVN
                     IPVNNEPAKLVSVRLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKSEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKQIDSKLETLTKSVV
                     NRFG"
                     /name="taxA"
     gene            30164..31393
                     /gene="taxC"
                     /locus_tag="E4F32_RS22280"
                     /old_locus_tag="E4F32_22280"
     CDS             30164..31393
                     /gene="taxC"
                     /locus_tag="E4F32_RS22280"
                     /old_locus_tag="E4F32_22280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase/nickase TaxC"
                     /protein_id="WP_134778717.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     ADKKAYTWTPTKEVTFKITGSGKTAVGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATVEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            31470..31763
                     /locus_tag="E4F32_RS22285"
                     /old_locus_tag="E4F32_22285"
     CDS             31470..31763
                     /locus_tag="E4F32_RS22285"
                     /old_locus_tag="E4F32_22285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO:0016987 - sigma factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_060598542.1"
                     /translation="MTEHDAICISGLHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            31760..31975
                     /locus_tag="E4F32_RS22290"
                     /old_locus_tag="E4F32_22290"
     CDS             31760..31975
                     /locus_tag="E4F32_RS22290"
                     /old_locus_tag="E4F32_22290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000760375.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="EexN family lipoprotein"
     gene            32331..32951
                     /locus_tag="E4F32_RS22295"
                     /old_locus_tag="E4F32_22295"
     CDS             32331..32951
                     /locus_tag="E4F32_RS22295"
                     /old_locus_tag="E4F32_22295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000157549.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERERG
                     SYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITSSNFSFYSTSAEKLLDSCEN
                     LSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEKKYV
                     VPGTRSNGGEQRKNRSHNASVIWPETILKSAFVDNSHPTKVIN"
                     /name="lytic transglycosylase domain-containing protein"
     gene            32965..33291
                     /locus_tag="E4F32_RS22300"
                     /old_locus_tag="E4F32_22300"
     CDS             32965..33291
                     /locus_tag="E4F32_RS22300"
                     /old_locus_tag="E4F32_22300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000008647.1"
                     /translation="MSFKKSVCNRISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLEKVKTGLSGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVAS
                     MMVN"
                     /name="TrbC/VirB2 family protein"
     ARG             19412..21033
                     /name="mcr-1.1"
                     /gene="mcr-1.1"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-1.1"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(23301..24172)
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          30165..31390
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.267"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3489..4555
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.719"
                     /coverage="99.0"
                     /evalue="2.69e-172"
     Typing          5098..5780
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4399999999999998e-137"
     Typing          5781..6685
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.338"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8817..10675
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.71"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtctacac tttataaagc gatgacacgc cctgctatgt atgtgggtgt tcctgtcgtc
       61 cctcttactg tggttgcagg ggcgttattt ctggcggggg tttatatcag caagctgatc
      121 tggctggcta ttcctgtcgc ggtttttgtt ctcagaatga tcactaaaca ggacgaccac
      181 atttttaacc tttactttct taagctgaaa atgctcggaa actcagtgtg taatcgtttt
      241 tttggcgcac gagcgttcct ctccggacaa tatgaagctg ttgaaattga tgagtttgtt
      301 aatgccatga aactgaatga gcgaatcact actggtaaat atatacccta ttcaagccat
      361 gttgataaaa atattgtcaa aacaaagaac ggtgattatg tagcgacatg gcaactgatg
      421 gggattaact ttgagtcaat ctctgcggag atgttagaaa ctattgactc acaggttgcc
      481 actcttgttc gttcattttc cggactgccc gtttcatttt ataaccattc atgcagggcg
      541 agtttttatg atgcctttac cacaaaatca ggtaataaat atgctgatat aatatcagat
      601 tgttactatg gttcgatgaa gaaaaataaa ttcaagggga acactcttta ctttacgctg
      661 atctacagac cagatgggcg tgtagaaaaa ctggaaaaac gcaaaaaaag tattaaagaa
      721 aagaaagatg atattaacat tcatgtgaag agaatgaatg aaatgattaa tacattctct
      781 ggcgctcttg ataaatttac ctgtaaactt cttggtatgt atgaggagaa cggaaaggta
      841 ttttcttctc aactgtcatt ctataattat cttctgacag ggaagttgca aaaaatcaga
      901 gtcactgatt ctccggtgta taacgttctt ggtggagtgg atgtattttt taatcatgat
      961 accggacaga tatgccgcat tgatggaaat aaattctttc gttctattga aattaaagat
     1021 ttctgtagtg agactgcatc cggtgttttt gatgtgctcc agtattctga tgctgactat
     1081 attattacgc attcatatac atccatgagc aaatcagaag cattgagtac cattaaacgt
     1141 gcggaaaaac agttaaagag tacagaagat gatgcggtta ctcaattgca ggaactggaa
     1201 aaggcaaaaa atgatattgt gtccggtgat atatcctttg gttactacca tttcacgcta
     1261 atggtaatgg cggacagtat cagggagctt gacgaatctg taagtaaaat tacggcagac
     1321 tttactgacc tgggcattat tccggcattg tcaaccatgt cattgccagc ggcatatttt
     1381 gcccaacttc cggcggtttt tcatttaagg ccgcgtttat ctccggtatc gaatgtaaat
     1441 tttgttgaac tggcatcatt ccataatttc taccagggga aacgcgataa aaactgctgg
     1501 acagaagcgg ttgctattct caaaacacca agcaaacagg cgtattatct gaatttacat
     1561 aactcagtac tgtttaaaga tgaaacaggt gaaaagaatc ttgctaatac taaagttata
     1621 ggaacggcgg gttcaggtaa gacaatgttt ctgtcatatc ttgcctgttc attacagaaa
     1681 tacaataacc ccgaaacttt tgctgattct gcaaaaaata agaagttaac gtgtgtcttt
     1741 cttgataagg acagaggggc tgaattatgt atccgaatgc ttggtgggga atattacacc
     1801 gtaaaaagcg gtgagccgac aggatggaat ccttttgctc tggaggcaac aaaacgtaac
     1861 cgtatttttg tcaagcaact gatggagatt ctgtgtacca gaaatggtga aagattatcg
     1921 acaagagaaa gattgcttat tagcgaaagt gttgatgctg tgatggattt tcctcctggc
     1981 gaaatgcggg aatatggcat taccagaatg cttgagcatc ttatgcagag agatgatcgg
     2041 gatgagcagg agaacgggat tatccttcgt ttgtcccaat gggctaatgg tcaggctcat
     2101 ggctgggttt ttgataatgc taaagacaca ttcaatattc agcatgtcaa taattttggc
     2161 attgatggca ccgagtttct tgatgatccg atggtatgtg caccaattac tttctatctg
     2221 ctgtatcgca ttacgcagct tctggacggt cggcgtcttg ttatcttcct ggatgagttc
     2281 tggaaatggc tacaggatga agcattcagc gactttgtct ataacaagct gaaaacaatt
     2341 cgtaagctga acgggctggt tattccggca actcagtctc cggatgaaat actgaagaat
     2401 aagatttcac gggctgtggt tgaagtatgc agcaccagta tttatctggc taaccctgat
     2461 gccgattaca atgattatgt ggaagggctt aagctgacac cagaagaatt taatattgtt
     2521 aaaaatcttg atccaatgtc gagacagttc ctgattaaaa aaagcagtct taaaaagggg
     2581 gatggtaaat cattttcagc acttgcgaca ctcgatttat cgggacttgg tggatatctg
     2641 aaaatattgt ctgccagtgc agataatctg gaaatttttg aaagtattta tcatgaagga
     2701 atggagcctg atgactgggt tcctgaatat ctcgaacggg caatctgaca ggtgaattta
     2761 tgaaacgtgt aaaaacattg atgctgatat cattattgac ggcttcattt tatagtagcg
     2821 caggaattcc ggttgctatt gatgccagtc cggaatgggc tgttgaagcc gcaagatgga
     2881 cagaacgact caaacaatgg tccgaaactg cgcaacatta tcagagtcag attcaggcat
     2941 ataaagacca gttagccacg gcaacaggta taaggaatat tgcggcattc acaaatgaac
     3001 taagtaatct acaatcagaa ctgacaaata tttataagca ggggaacagt tatatttctg
     3061 attttaccag taatcccgaa ggtgcattga gcagccaggc taaaaatctg ttcagcaagt
     3121 atggagcctt cgatatgtgt aatacgggct atgaacgtaa tgataatctc tgtaaagcac
     3181 ggattgtgtc aacggctgcc agtattgaac agggaaatga gatcaataag cagttatcaa
     3241 gtgcaatgtc tcagattcag agtttaagta gccgaattga ggcttcaaag gatatcaaag
     3301 aatcccagga tttggctaac gcattacagg cgcagtcgtt aaaaatgcag gcgataaaaa
     3361 tgcagtatga tgtctggaat aataaaaata aagcagatca tgagatgttg gtcacacaag
     3421 aacaagaggc ctttattaaa cagcagaaag aagctgaacc attaactttt gattaaaagg
     3481 tggatatatg gctcaggggt ttttcgttaa atataattct accgttatgg attcggtaga
     3541 taaaatcagc tccagttatc aaacacaatt tgctaatgat attatgtcgt tagcaacggt
     3601 atcggttacg ttatatgtac tatggaaagg atatcaaata cttgccagta aaactcagac
     3661 gcccttgcag gatttagtat gggatctatc aaaatttgca atcataatca tgtttataac
     3721 taatgcggat gggtatttaa ctgcggctac tgatgcgttg caaggcatga aagatggttt
     3781 ttctggaggg gtgagtgtct ggcaaacttt agataactta tggaaaagta cgcaaaatct
     3841 tggtgcggaa atatactctt tagataaatc aacatatgtt aaagatcaag gtgtagttgg
     3901 tcagttttta atatggactg gctcgcttat tttaatggct gttagtgtgg ttgttttttt
     3961 aacagcagat gtaacgatga aactcctgat tattacagca ccgatattta tcttttgtct
     4021 catgtttggt tttattcgga gcatgtttaa taattggtta caatcgttat tctccagtat
     4081 tctgacagtt ctatttgcca gtttagtgat tcggatagca atggattttc aagggatgat
     4141 cctttcccat gctattcgtg ctgcacaaac agggaatgta aatcttgtat ctacaggtgc
     4201 tatgggcttt atggctgggt ttttgggggc tttattggtt cttatagcta aaggttttgc
     4261 tgtccagtta gcaggtgcag gtgttgaagg tgctgttcag ggcgcagcca tgatggggct
     4321 gggtgctgct ggtatggcta ctggtaagtc tctgatgctt ggcggtcgtg ctggtttagg
     4381 ttttggctta gggatggccg gaagaacggg catgaattct ttatcaggga aagcagggaa
     4441 tctgatgggg cgtggtgcgc gaacagccgc tgaatgggcg ggtgaaaagg gatatcaggc
     4501 gctggctccg acaggtgctt tggggatgaa ggcgagaaga cttgcatccc tggaaaaagc
     4561 acgagcgagg aacgccgcat gaaaataatg aaacgttttt tttatgtgat gtccctgaac
     4621 tggatactgg tgttcgttga taaacatatc acctccggat ggagcaggaa gagaaagcta
     4681 ataacccagc ttgttatttt aatcacggga attaccttat ttaaggtctt aggtggaatc
     4741 agtcagagct ggttgtgaca tgcttcatta ttttgtcatg atactttatt ttgtcctaat
     4801 ccttctttta ggagtgttta cctgcttcgc aggaataaga cttggtttag ggcttgcggg
     4861 gaaatggaga aagggtatgg atcttcgctt tttgcttctt attggtggga ttctttttct
     4921 tcatcttggc ctttatctct taaaggtgtt cgtgaacgct taataaggtg aatttatgcg
     4981 atgtgtgatt cttttgtttt tcctgtttca gatttccgga tgtaccacaa ggaataacct
     5041 tccttctgat gtttccggcg aactggaacc cattaaccac agccaggtaa tcagttatga
     5101 gtgaaaaaat cgaaaagaag attgagacag ctaaatcctt cgaaagacaa ctctatgcag
     5161 aaaatgaacg gtctaagaaa aatgcatggc gtgttgcagt ggcagcatca ttactgtcct
     5221 tatgtcttgg ggctgcaatt tgtgttcttg tgccattaaa agaaaaagag cttgctattg
     5281 tggctgttgg cgaaaaaaca ggccgtacag aactgattac tcaggttaaa caggaaaaga
     5341 tcctgcaatc tgaagcactt gggcggtatt ttgtaaacac gtatattact ttacgtgaag
     5401 gttataatta tccgtctctt cagtatgatt atgagactgt acagctatat agctcgaata
     5461 cagtaaagga cgattatctt cgtctgtata acagcgatat ggctcctgat aaaatttatc
     5521 ataataacgg ttcttacgtt gcggttgagg tcatctccaa tattatttct gacgcaaccg
     5581 cgccagataa actggcctct gtgcgcttta aaaaaacgac gagaaacttc acgaccggac
     5641 aggtttcagt ttcatactgg acggctcgtg tgacatatcg ttttgaacca gaaaagtcgg
     5701 ttaaaagttc cagtcgtgag cttaatcctc tcggttttac tgtaaccagt taccagacag
     5761 atcgggaagt caggggggaa tgatgaagaa attatttatt gctacatgtt tattgcttcc
     5821 tgggcttgtg tattcagcag cgactccgtt accttctgga tttgatgcaa ggatgcagac
     5881 tgtgagttat aacggtgcga atacgaccgt tatccggtca aagacaggat ttctcacatc
     5941 agttgttttt gatgaaggtg aggctgttat cagtgcaaag gctggttttc ctgccggatg
     6001 ggaaatcaca accgatgata atgttgttta catcaatccg cgtcccgttg ttcaggaact
     6061 ggaaggcgat gaaggcgaaa aactgaaaaa ggttttccag ccaacagaaa aggaatggga
     6121 tacgaatctt tttgtcagaa caacaaagcg gatttacagt ctggatctaa tccttctgtc
     6181 agaagaaaaa caagcgcaac ctgcttatgt tgtacaattc cgctatccat cagaaatagc
     6241 gaaaaagaat gcagaagaag taaggctggc aaaagaaaaa caggaaaaac tcagacagaa
     6301 gaaactgatt tctgaaagtt ttgaaaaagc cgatgcgcct aaaaactgga attatttcat
     6361 gcgagtcaat gaaaaatatg acagtcgtcg tatcgcccct gattttgctt atgataatgg
     6421 catatttacc tttttaggtt ttaactcagg gaaagtattc ccagctccat ttgctgtaag
     6481 agatggtcag gaacagacac ttgcttttaa tgtggaaaca aaaggcaagt ataaaattat
     6541 ggttatccac aacgttaatg acaaatttgt tttacgctat ggaaacagcg ttgttggtgt
     6601 tgtgaataaa tctttcggaa aagtattaac cgaccagagg aatacatctt ctcctgcggt
     6661 agaacgtgtt gaggtgaata atgactgata tgaaaaaaac agatgattta gttgatgatg
     6721 cgaatcagtc aaagctggaa agtaaacccg ttcatgatat cggtgatatc aggaacaaaa
     6781 ataatcgtat gcgttctggc tttctgtttg gtctgatggc tgtattagtt attggtattt
     6841 ttgcgctgaa aacatataaa aattattttg cagatgacca gcagaaagca tcagaatcaa
     6901 caggcgatac cagcatttct caggtaagta aaatccgtac tggtcttggt cagaattttg
     6961 accctgttga gaacaaagca attatcaatc caggcgctac tggttctggt aatactgttt
     7021 ctgacggtca taaagaagtg ccacaggagg ggttcaggaa atatttgtca atacctgttg
     7081 ctggtcaggg agggagtcag actggcgcga gtggcagcag caggacatcg cagacatcag
     7141 agccacagga agaaaggaaa gaatcaaaag aaaatgtgcc tggtaaatca ggaatgaaag
     7201 taactgcgat taatctcgat cctgatctct atattgagga aaacaggctt ataccgtgtg
     7261 ctttaaccac acgctttgtt tctgatgttg caggtcgtat cagttgtgtt tttacagaag
     7321 atgtatggag tgctaatcac cacacaaaat tactggaaca gggaactaag gcttttggtc
     7381 gttaccagac cggaacgctg aatcatggac agggcagaat gttcgttatg tgggagcagt
     7441 tgcggacgcc tgataataaa cgaatcgata tggtaaatac agcagcagct ggtcccctgg
     7501 gagaagcggg cattgatgga tggatcgatt ctcacttctg ggagcgtttt ggtggttctc
     7561 ttatgctcag tatggttcag gatgtagcgg cggcggcggc ggataatgca cctggtaaag
     7621 atcgtaatgt ggattatacc gaaaactcac gacaggcaat ggctgaaatg gctaaagtcg
     7681 cacttgagaa ttcgataaat attcctccaa caatgtataa aaaccagggt gacataatca
     7741 ctattatggt cggggaagac attgattttt ctgatatcta tgagttaaag gtaaaatagt
     7801 atgagtggcg ttattcctgt cagcattagc aataaatcgc tcgattttta taaaaatcaa
     7861 gtattccagt cttttcttga tattgaaggg ctaacagaaa ttgctgtcaa ccgtcctggt
     7921 gaaatctgga cggaaattaa tggtgactgg acattccata ataatgacag tgtgaccttt
     7981 gatttttgta aacgtttttc tcatacgctg gcatcatttc ggggagatga aatcggcgag
     8041 accaagccat tactttcagc aacactggaa tcaggagaac gtgtacaggt cgtttttcct
     8101 cctgcatgtg agagaaatac tatttcaatc actatacgta aaccatcgac acggcagata
     8161 acacataaag aatatgtggc gaacggtttt tatgattatg tccagtcagg aaaaaaacac
     8221 aggacatacg atgatgagct tcttgattta ttcagagcga gaaatatcgc tgcttttatg
     8281 gagcttgccg tagcagcagg gaaaaccatt gtatttgccg gagcaacggg ttccggtaaa
     8341 acaacttata tgaagagtct tatcgatttc ataccattga atacacggct gattacgatt
     8401 gaagatgcag aggaaatcaa attttttatc cataaaaatt atgtgcatct tttttaccct
     8461 tcggaatccg gcagtgattc cggctctatc ataacagcag cgaaattaat caaatcctgt
     8521 ttgaggatga aacctgacag aattttactg gcggaaatta aaggtggcga tgcctgggat
     8581 tttgtgaaag ttgctggttc tggtcacagt ggaagcatga cgtccattca cgcggcctcg
     8641 gcaaaagatg ccatcattca gatggtgaca aaatgttatc agaacagaga atgtcagaat
     8701 cttccttttg atgttcttcg taaaatcatt atggacagta ttgatattgt tgttcatgta
     8761 ggcagggatg gtgttgtcag gcatatgagc gacatctatt acaaaggggc tgaatgtgaa
     8821 aacttttaat aagtcgcaat ctgtttttat attcgctgtt atgcttgttg cggcatggtt
     8881 tgcaggttca tttattttct ttgtgcttta cttcagccag ataaagaatc tgaaaccatt
     8941 gaaggctgtg ttcagatcca ttgatgcgta caggatggat atttttacag acagccttac
     9001 tttatctgtt gtgactacgg atatcagagt tttagccttt gttgcacttg gagcaggatt
     9061 tgtcgtgtct cttatcatcc ctgttgcggc gttgataaaa ctgaacgaga agaaagaaaa
     9121 tattttcggt gatgcgcgat ttgcaacaat taatgatatt cgtgagtcta acagtttcac
     9181 gcttgacggt gacgaaaaag acgggattat cgttggtata aaagacaaga aaatcatacg
     9241 ctatgtggga gcagcattca gtgctatggg agccggaaca agggctggta aaggtgctgg
     9301 tatagtcatc actaatctta tgaagtactg gtggagtgtt attattctcg atccaaaaag
     9361 ggagtgtttt aatatcacca gtcttatcag gaaggtgatt ttaggtcatg aggtttataa
     9421 atttgatccc tttagttcgg taacgcaccg ctttaaccct ttgtattatg tcagcatggg
     9481 aacgagtgaa ggttttaatc agcttgaaaa tctttcgttg attatttacc cctataagac
     9541 agatggtgcg gatgctggta gctatcttaa taagacggcg ggtggtgttt ttaaatctta
     9601 tgctgtcgct ttatggttta tgattaagaa tgataaggct ggactaaaaa cgcttgatat
     9661 tgagcctgtc ttctcaatgt ctaaaattaa tcagcttttt gagcgtgcag aacccgaaca
     9721 tcttctttca ttcatgaatg atattcgttc agaattaagg gggaaagaca aaacgcttgc
     9781 tgatattggt gtcgctggcc ttaaggcatt cattgagatg gaggataaaa caaaagcaca
     9841 actcaaaagc aactttctta atggattatc accgtttgct aaccccaatg tggcaaatgc
     9901 tactgatgga aatgattttg atttgcggca ggtgcggaaa aagcggatga caatttattt
     9961 ctgtatttct ggtgataatg ccaggttggc tgaaaaaata acaaatattt tctttcagct
    10021 tgctatacag gtgaatcttg aaaagatgcc aacggatgat ccggaaataa agcatgactg
    10081 cctttttcta ctggatgaat tcccgtcaat tggtgctgta gattacatca agagtaaatc
    10141 tggtctcatt gctggttaca agctgaaatt gttgattgtt taccaggtcg gctctcagct
    10201 tgaggaaatc tacagttatg caggcagtaa aacattactg gcaagtgcac cctgcaaaat
    10261 tgtttattcc gcttctgatg ttaaagatgc gcgggagtta tcagaagcga tggggacacg
    10321 aacagtaact atcggttcga aaagcaaaag tcgtagtcgt ggaggaacat cgagatctga
    10381 atcagaaagc cttattgagc gtccactggt aacaacaaat gaactactta cactgaaatt
    10441 tagtgaagaa atcctggcaa tgaagggaga aaacccgatt cgttgtgaaa aggcattcta
    10501 ctatctcaat gagtatttct ttggtgactt tgtgaaggtt gctccggaac ttgcaaacat
    10561 tttccccagg aagaaaggaa agctggttat gcctccacag aaagtatttg aaaatgaaat
    10621 cgtcgcaaaa gggtatctgg tagtaaaaga tgttcctgat ctcgataaag cggcataaag
    10681 gagaacatga tgaagaagtt agtgttttta ttgatttcca ttcttgctgg ttgttcatca
    10741 ccgccagagc caacacccgt gcagtttgaa aaggcaaatg aagtgatcaa tccctcattg
    10801 ccatatgtcc ccgattttca cggtgtcata aagtcagacg ttagcggtaa aggatgggtt
    10861 tatgagatca cttccttatc cggagtacag gccagaacgc cgacgtttta ttacgctctg
    10921 gctcatgcgg acaggattgt agtgacaaca catgatgctg ggctgtggtt tcgaatcaga
    10981 gatctgttga agatggaagg ggcaacagca gtcgtggaat ggcgaaacga gaaaagtttt
    11041 ttacctgaac aggtaaaaat agttttcatt aaagcacaaa atgaggtgaa aaatgactgg
    11101 aaaaaataga gtggttttac cagccttgct ggcgttagga ttaatatcaa caaatgctaa
    11161 tgcatctgac ccttgcgctt cggtactttg cttatatgga aaagcagtcg ggcaaggtgg
    11221 agggagtgaa tgcagaagtg ctgaaaagga tttttttaat attctgaaaa agaaaaaagg
    11281 aagcattcgc tggagtaaaa cttttgatgc ccgtaaagca tttctgaatc agtgttcaac
    11341 ggctgatcct gctgctatat caaaaatcat gagtaagttt ggtcgtgtca gaggttaatt
    11401 aaaacctgat aaccgcgcca acgggtatca gggtactgca agtatcacat ttcgcgccaa
    11461 cgaaatgtga tgattagtta caatcactga ggttagaaat gaaagtaacc aaatctatta
    11521 tagctatttt attgtgctta tctgctggta gtgcataagt ggatgtaata aaaaatgctc
    11581 gtgccttttt gaaaggaacc ctcgatctga gcgttcagga agcagatgaa gatatagagg
    11641 aaagggaaga actttataaa aagaacgggg cacaacctga ctatttaagt tacctggagg
    11701 attaacacat gcaatatgca ttgtttgatg gatttgaacg aaagtttttg ctggatgctc
    11761 ttgaattcgg tgttctgaag gactggaaag aaaatccggt aaaagaactt cctgatattg
    11821 atgaatctgc tcaccccttc catatctgtt atggtggata tttattaaac cctggtgttt
    11881 cagattcaga tattagcaga aaaataaaag accaggcagg attctggctg gcagctattg
    11941 atgatacccg catggattgt cattcaatag cttattatga tattcacaca ctccctctga
    12001 tttcgtgtgg tcatcaaaag atagttcctt ttgccgcgtt aataaaagct gatgaatgca
    12061 tcatttcaaa aattgcttcg tattctggtt ttgctgtaac agcctttttg agaattaaag
    12121 agtgggatat tgcaactaat atacttaacc gtgagggaat ttttgccttt aatggctgtg
    12181 agcgcagatt cagagtagta agtaaagata actggcaaca tacagtatcg gaagaacgcg
    12241 ctatccgttg tgccaaaaga ttaattcaat gtaaaggata acaaaatgag actttttatc
    12301 gcagaaaaac ccgcagtagc aaatgatatt gttaaggcac ttggtggcaa ttttacccgc
    12361 catgatggct ggtttgaaag tgataacgcc attgtgacta actgttttgg tcatattatc
    12421 gaatcacaac cgccggaaaa ctataatcct gaatacaaag cctggaaggt tgaaacgctt
    12481 cctttacgtc tttatcccgt gaagtatcag cctgttgaaa gtgcagcaaa acaggttaaa
    12541 acgattctcg aacttatcag acgtggagac gtgactgaaa ttgttcacgc tggcgatcct
    12601 gatgatgagg gacagctact ggttgatgaa gtcctggaat atgcaggcaa cacaaaaccc
    12661 gtaaagcgcg ttctgattaa cgacaacacg cttccggcag tgaaaaaggc actggcaaat
    12721 cttaaagaca atcgtgattt caaaggactt taccttaagg cgttggcgcg gtcagttgcc
    12781 gatgccgtct atggcttctc tatgacacgt gcgtacacca ttccggcaaa agccagagga
    12841 tatcagggcg ttctgtctgt cgggcgcgtc cagactcccg ttcttggcct gattgtgaat
    12901 cgtacccgtg ctaaccagaa ccataaatcc agtttttact acaccatgac cggagtcttt
    12961 cagcgtggtg ctgatgttat cagggcgaac tggaagccag gggaatttgc tccgctgaca
    13021 gaccgtaaat tacttgataa agcgtgggca gacggaacgg ctgcatccct tgcaggaaaa
    13081 ccggctacag ttgaagcagc agcaactgat gataaaaaaa cggctgcgcc gttgccgttt
    13141 aatctggtca gactccagca atacatgaac aagaagttta aaatgacggc acaaaaaacg
    13201 ctggatatta cgcaacaact acgcgaaaaa tataaagcaa ttacgtataa ccgctctgac
    13261 tgctcatatc tttcagatga acaattcagc gaagcgccgc aggttatcga tgccctgaaa
    13321 tcagtctttc ctcagtcgct ggatattgat tctgcacgta aaagcaaagc gtttaacagt
    13381 gcaaaggtga ctgcgcatac tgcgataatc ccgaccgcca gtgtgcctga tgttaacgca
    13441 ctcagcaccg acgagcgcaa tgtttacctg gcgatcgcac aacactatct tgttcagttc
    13501 atgcctgaaa aagcatacca ggaagtatcg gttgccattc agtgtggtga tgagtcgttc
    13561 tatgcccgtg ccagaaaaac aactgacagc ggatttgagg cgtttcttgg cgcggaaacc
    13621 acagacgaag gtgaatcaga agataatgat gattccgctt ttgaactgct ctgtaaaatt
    13681 cgcacaggag aaacactgac gacaaaagaa gttattgtta atgagaagaa aacaacaccg
    13741 ccgccgttat tcaccgaagc ctccttgctt gctgcgcttg ttcgtgtcgc ggattttgtc
    13801 actgatccaa caattaaaaa attgttgaag gataaggata aagacaaaaa agatgaacat
    13861 ggtggcattg gtacgccagc tacccgcgca gccattctgg aaacgctgaa gaaaagaaac
    13921 tatatcacgc tggaaaaagg gaaacttatt cctactgata ccggatatgc gcttattgat
    13981 gccctgcctg gtatagcggt taatcctgat atgacagcat tatggtctga aaagcaggct
    14041 gccatagaaa atggcgacct gacggttgaa cagtttatta atgatctgta cggtgaactg
    14101 acaggcatga tttctgatgt tgacctgggc gagatgaaga ttgaacccgc tgcgccagca
    14161 gggcagtttc aacgcctgga ctctccctgc ccttcctgtg gtaaacatat tgttatcagg
    14221 ccgaaaggtt atttctgtac cggatgtgaa tttaaaatct ggagtgagtt ttctggtaag
    14281 aaaatcaccc aggcacaggc cgaaaaactg gttaaatcag ggaaaaccga tttgattaag
    14341 ggatttaaaa agaaaagtgg tggaacgtat gatacagttc ttgtccttga ggataagaaa
    14401 acagggaagc tgggttttcc ggcaagggct aagaagtgaa aacaaagcag gaatggcttt
    14461 ttcagttaag aaaatgtaca tcaagagata ctcttgaaaa agttattgag attaaccgtt
    14521 acaaactgcc tttatcagaa tcagaggcat tttattctgc cgcagatcat cgccgagcag
    14581 aactggtgat gaataaactt tatgataagg tgccttccgg cgtctggaag tacgtacact
    14641 aaataagagg attaattata tgagcgaact gactaaagaa gatgaatacg gcattatcag
    14701 ccgtactatg atgaatattc gttcattgcg tgtttttgcc cgtgagattg attttgagca
    14761 gttgctcgaa atgcaggaaa agctcaacgt tgttattgaa gaacgtcgtg aagatgctga
    14821 acgtgaagcg gctgaacgcc aggaacttga agccaaacgt cagcaggcca ttgaatacat
    14881 catatcttta ggtcttgatc ccgaatcact ccttgctcct gtaacagcag atacagtaaa
    14941 aaccaggcgg aaagccaaag gcggagtaag aaaggctaaa taccgcttta aggatgaaaa
    15001 tggcgagatc agagaatggt ctggtaatgg caagcgtccg cttgctttac agaagctgct
    15061 tgatgagggg catttcatgg aagatttcct cattgagaaa actaagccgg aacaggcaga
    15121 ataacccgcc aagtatacat cacacagggc actgttgcaa atagtcggtg gtgataaact
    15181 tatcatcccc ttttgctgat ggagctgcac atgaacccat tcaaaggccg gcattttcag
    15241 cgtgacatca ttctgtgggc cgtacgctgg tactgcaaat acggcatcag ttaccgtgag
    15301 ctgcaggaga tgctggctga acgcggagtg aatgtcgatc actccacgat ttaccgctgg
    15361 gttcagcgtt atgcgcctga aatggaaaaa cggctgcgct ggtactggcg taacccttcc
    15421 gatctttgcc cgtggcacat ggatgaaacc tacgtgaagg tcaatggccg ctgggcgtat
    15481 ctgtaccggg ccgtcgacag ccggggccgc actgtcgatt tttatctctc ctcccgtcgt
    15541 aacagcaaag ctgcataccg gtttctgggt aaaatcctca acaacgtgaa gaagtggcag
    15601 atcccgcgat tcatcaacac ggataaagcg cccgcctatg gtcgcgcgct tgctctgctc
    15661 aaacgcgaag gccggtgccc gtctgacgtt gaacaccgac agattaagta ccggaacaac
    15721 gtgattgaat gcgatcatgg caaactgaaa cggataatcg gcgccacgct gggatttaaa
    15781 tccatgaaga cggcttacgc caccatcaaa ggtattgagg tgatgcgtgc actacgcaaa
    15841 ggccaggcct cagcatttta ttatggtgat cccctgggcg aaatgcgcct ggtaagcaga
    15901 gtttttgaaa tgtaaggcct ttgaataaga caaaaggctg cctcatcgct aactttgcaa
    15961 cagtgcctca cacagacata cacataggta tgtctgtgtg acatacctat gtacagtgcc
    16021 agatggcgtt gctacgtgaa aaataaccta ccctacccct gcttttttcg cagattttac
    16081 gtagcacacc gttgctatag cattcatcta aaaagctgaa tgtccttttc tgtcgctgtt
    16141 gcattttaat tcactgaaaa atattgagta atgaaaatgc cggtaacgcg tcaagctgtt
    16201 ggccaacgcg acaggcagta ttggaagcaa caaaaaaaat gaaagaaaaa tccctcaaat
    16261 gaaggagaaa aaacggaact acagccgaac gacaggcaca aaaaaacccg actggtaatc
    16321 gggctttttt gcgttttcca gtcttgagtt ggtggctctg accggagaag gttactctac
    16381 acctaacgtt tacattttat tcactatggt gctgtaaagc aactaaatgt ggttaaggaa
    16441 ccacatttag tggtgtaacg ggtgttttgt gtagcgtata atcagtcagt tagtcatgta
    16501 gttggcttgc aatcctgctg ttcccgtatc tgcgggtatg aacgatttta aagtcctggc
    16561 tggataaaca ggtggtgctg tattcggtgc tctggcagga ggaggtcact ctacacctaa
    16621 caatgcgaga gagccgttgt gacaggcagt gtatgagcaa gcaacggtga tttcctccag
    16681 ttttactgga gaaacggatg attaatatgt taatcatcgt tctcagagct gtggtcgccg
    16741 ttgcaaacgc gctgattgca gttctggaac tgatccgcca attcatcgat tgatgacaac
    16801 ggaaacgtaa ctaagggcag gaagtgaacc gcttcctgcc ctttaacaaa aaaggaggat
    16861 ttatggattt tatgagcatt ttggggcgct gtttacttct tcctttccct gttgcgatgt
    16921 ttgtcggttg cctgtttcct ggcatcgatg atcgcttatc cggaatgctt atgagtgttg
    16981 ctgtgggatg tttatcgtgg tatgtcacca gaccgaagaa ccgacagaca aaggccgcgt
    17041 aagtggcctt taattgggat accctttagt tacaacattc taatagctca ttccatcctc
    17101 cgctcatcca gcaaacataa cctccctgcc cctccccact acagaaagtg gcgcgccccg
    17161 cccgtacggg cggaatctaa tcgcttaatt ttgaatgttg taactaaaca tactcattgc
    17221 tgtattgcca caaggtttcg cagcaaggga ggataccgaa agtaacgctg tcacttttgc
    17281 tttcaagtga ccaaccaacg ggtacatcac actgacatac aataatgtat ttcttcatga
    17341 tgtacatcat tgtaattcat tgtgacatac actatagtat gtccatgtga catacgcatg
    17401 cgtacatcac atagaaatac acatgagtaa atcactgtga catacaaggg gtactatgat
    17461 tatcgtaatc ggtggtgata aaggcggtac aggtaaatca catctggcaa ccaacttaac
    17521 tgtttgcctc tcacaacagg gaaagagaac ggggcttgtt gaaacagacc tgaatggctc
    17581 gaccaaaaaa tggaataaac gcagagaaca ggccggatta ccgcccgtcg cattaaatga
    17641 agcctatggc gacatcagtg ccaaaataac aaaaatggca gatgtagcag aaatcctcat
    17701 tatagatact gccggatatg acagcacgga gttcagaact gcactaaaag ttgcagacat
    17761 tgtgattgtc ccgatcgacc cgcttgccca ggtggaagca gacagtcttc agactgtgac
    17821 aaaaattgtc agggatgcgc aaaaaataaa tcccaggctg gctgcacatg tgctcctgta
    17881 taaatgccag ccaaatacgt tcagtgaaca acaagaatta agagactctc tcaacagcca
    17941 tgattactgg cttaaaccaa tgaaaagcac ggtttctttt ctccgggcct ttgtaagagc
    18001 aatgaatcag ggaatggggg ttcacgagtt aaaaagtaat gttagcggtg ccagccaggc
    18061 aaaagcacaa atcgaactct tgctgaaaga actggaactg taaatcatag tgacatacac
    18121 taatgtacat catcatgata tacattagtg tgaaacaatc agattagaag gtacatcatg
    18181 gcaaagctgg aactaccaga actagaacaa aaccaggaag tgaacgccac aaaatttgtt
    18241 caggacgcag gaaaacgccc cacagagaac aaaacaaaac tggcttcatt ccgcataccg
    18301 aatgaattgc ttgaattcat tgataacgaa tcaaaaagac tgcatcgtcc caagacaaaa
    18361 ataatcaaag cagcattact ggcttatcag gatctggacg aaaacgaact cattaatttg
    18421 tggttcagag ctgacagaaa tgattaatca tagcaagtgg cccaataaac tctttatcat
    18481 tttcttttag atacttatct gaatgtacta acttcatcac ttttaaatgt agttgtgatt
    18541 catttttagt tactcttgca ataaaatacc cttgtacagt tgttgcacgg gtatttagga
    18601 gcactagatg agccttatcg atttaagttt atcaggatta tcagaaccag gaacaaaact
    18661 tattgaaaaa ataagtgatg ctattggtgt gctttatgaa cctacaagaa tcaggaaaaa
    18721 agcgaaggct gaagctgaag ctaaacgcac agagctaatt tctaggttag agcttgaggg
    18781 aatagaaaaa cgggcagttg agcgttttct caaacgtgaa acgaaacgac aggaaaacat
    18841 tgaaaatata acaatgcaag cagcacaaag tctatctgag agtgataatg tttctgatat
    18901 tgacgaagat tggatagagg ctttcttcag ggagtgtgag gatattagtg acgaacaaat
    18961 gcaaatgctt tggggaagaa ttttatccga agaagcaaag tcaaaaggtt cgtttagtcg
    19021 tagaacctta aaactattat ccactataag caaagaagaa gcgaacctta ttacttattt
    19081 tggaaagttt gtttggcagg caaacaaact cactccaatt ttatttaccg atgagaatgg
    19141 agatactgaa ggtataactt tcgataaact ttcggtttta gactccttag gcgttattca
    19201 acaaggcatt ggttatagtt taagatacaa attataaata ctctcaagtg tatattcagt
    19261 atgggattgc gcaatgattg cctaataaaa tttctgaaat atttctgtat cgcataattt
    19321 tttatatcag ataaattgta ctggatttct taaaaaattg cagtatgatt gccgcaatta
    19381 tcccaccgtt tattttttga gtagtttctc atgatgcagc atacttctgt gtggtaccga
    19441 cgctcggtca gtccgtttgt tcttgtggcg agtgttgccg ttttcttgac cgcgaccgcc
    19501 aatcttacct tttttgataa aatcagccaa acctatccca tcgcggacaa tctcggcttt
    19561 gtgctgacga tcgctgtcgt gctctttggc gcgatgctac tgatcaccac gctgttatca
    19621 tcgtatcgct atgtgctaaa gcctgtgttg attttgctat taatcatggg cgcggtgacc
    19681 agttatttta ctgacactta tggcacggtc tatgatacga ccatgctcca aaatgcccta
    19741 cagaccgacc aagccgagac caaggatcta ttaaacgcag cgtttatcat gcgtatcatt
    19801 ggtttgggtg tgctaccaag tttgcttgtg gcttttgtta aggtggatta tccgacttgg
    19861 ggcaagggtt tgatgcgccg attgggcttg atcgtggcaa gtcttgcgct gattttactg
    19921 cctgtggtgg cgttcagcag tcattatgcc agtttctttc gcgtgcataa gccgctgcgt
    19981 agctatgtca atccgatcat gccaatctac tcggtgggta agcttgccag tattgagtat
    20041 aaaaaagcca gtgcgccaaa agataccatt tatcacgcca aagacgcggt acaagcaacc
    20101 aagcctgata tgcgtaagcc acgcctagtg gtgttcgtcg tcggtgagac ggcacgcgcc
    20161 gatcatgtca gcttcaatgg ctatgagcgc gatactttcc cacagcttgc caagatcgat
    20221 ggcgtgacca attttagcaa tgtcacatcg tgcggcacat cgacggcgta ttctgtgccg
    20281 tgtatgttca gctatctggg cgcggatgag tatgatgtcg ataccgccaa ataccaagaa
    20341 aatgtgctgg atacgctgga tcgcttgggc gtaagtatct tgtggcgtga taataattcg
    20401 gactcaaaag gcgtgatgga taagctgcca aaagcgcaat ttgccgatta taaatccgcg
    20461 accaacaacg ccatctgcaa caccaatcct tataacgaat gccgcgatgt cggtatgctc
    20521 gttggcttag atgactttgt cgctgccaat aacggcaaag atatgctgat catgctgcac
    20581 caaatgggca atcacgggcc tgcgtatttt aagcgatatg atgaaaagtt tgccaaattc
    20641 acgccagtgt gtgaaggtaa tgagcttgcc aagtgcgaac atcagtcctt gatcaatgct
    20701 tatgacaatg ccttgcttgc caccgatgat ttcatcgctc aaagtatcca gtggctgcag
    20761 acgcacagca atgcctatga tgtctcaatg ctgtatgtca gcgatcatgg cgaaagtctg
    20821 ggtgagaacg gtgtctatct acatggtatg ccaaatgcct ttgcaccaaa agaacagcgc
    20881 agtgtgcctg catttttctg gacggataag caaactggca tcacgccaat ggcaaccgat
    20941 accgtcctga cccatgacgc gatcacgccg acattattaa agctgtttga tgtcaccgcg
    21001 gacaaagtca aagaccgcac cgcattcatc cgctgatttc tccctgtatt ttttccaaac
    21061 ccaccgcaca ctccattcgt attatgggcg gtggggtggg gtttgttatg ccgtatttat
    21121 caaataaacg cctacttgct gagatgagta tcgctcttgt catggcgatc gttgccacgc
    21181 tgacccttga gcacagtcag attgatctga tggtcgctga ttggttttat ctgggtatgg
    21241 ggcattggat ggttgccaag caagcttttt tgccagattt gctactgtat tctggactaa
    21301 aaaagctgct gatggcgatg ctgatctact tgctggttgc gaccatttgc cgtgcttatc
    21361 atgagaaaaa gggcaatgct atcactgcca agtggcttgt cccagtgaca aaatttcgcg
    21421 tgcgtgagct tgcgtatctg gtgctgactt tgatcctagt gccgacagtt gtcgcgtcat
    21481 tgaaggcata tactcatgtg gtctgccctg tgcatttgac gatttttgat ggtacgctgc
    21541 cgtatttgcc gatgcttgat agtatgcgta acaccattcc tgataagtgc tttcctgcgg
    21601 cgcatgccag tagcggattt gcgctgtttg cctttgcgtt tgcgccaagt ttgcgccgcc
    21661 gtcgtggtgc gatcatcatc gtggtgatgg cattgggctg ggcgatgggc tgctataaga
    21721 tgattattgg cgatcatttt ttgagccata cggtggtgtc gatgatgctt gcgtgggcga
    21781 tgtcggcagg gcttgcgtgg gtgtttttta agaagggtga acaagtttaa tcggctttaa
    21841 aagtaaaaac ggtcgtatcg gttattacgg aacggttttt tcagttgagt ttcaaagcga
    21901 tgattcatca acctggaaac taccaatagg aacggcttta cttacgccta tcggtcaaga
    21961 attgatgaaa atatgcggtt ccactccaga tattgcgtat ttaaataaat tcctcaacaa
    22021 aataaatgtt gaaggaagcc aggtaaaact atcaattatt aatatgtaaa gaggtcgagt
    22081 tacctgtctg agttccacta accataatta aaagcgaccg caaaaactgc ccagtgaaaa
    22141 ctgtgttgcc agtaatcaca aatgcagcaa gtactaataa agagcctgtt aagcccgcaa
    22201 atatgtgggc ttatttttaa ttactcttca tcatcccgca ggaactgaca aaaatcttcg
    22261 ctccccatca tatcgacagt gtttttaaat tcgaaaaaag aaattcctgt catagcacac
    22321 acgcgctgga taatcagttt tttcttttgc tgtctgacgg ttgcggtttc accaaactgg
    22381 ataacagcca ggactgtttc atcatatgat ttcaggacaa aatcacaggt tatattctgc
    22441 acttcacgga ttaaatcttt tacagcttcc tcgctaccat gaggacgaac aaattcaatg
    22501 agccgtattt ttggaaaaac ggaataatca ggtataatat tacgtgaaag aatgcctaaa
    22561 aactctgatt cattatttga gagtaagggt actttagtaa aacaatcaac ctccccaatg
    22621 gctgtaaaag cattggttgt caaagctgga ttgttcttaa taaaatcaga gaatacattt
    22681 tcagctgatg gcaaaacaac agcagaatta tgttgttcat ctcgtttttt attacggtta
    22741 aatatcattt catattcctt ttaaaagagc ctgcggcagg cacaggcaaa aacatcgttt
    22801 ttggacaatc tgattcaaat gttaacattt gacatcggat tgtctatcct gcattgttct
    22861 ttactgtctg taagatcggc gctcattctt ccggcgttct cccgatacca aaaagacgga
    22921 acgcaaaacg gctttcgatc caccattcaa aaatctgctc tgcgcaacca caaacagtta
    22981 aggcgatcca tgcagcaaga acaacgtaac tactgacatc tgcacccatg taccaggcga
    23041 tagttaaacc catcactgcg ataccgccta aaaactgcaa caagaaacga aatcccgcaa
    23101 gaatccccaa tggaataaca acaaccagat aaagggaagc agccagaagg tatctgacag
    23161 gtgtcagcag ataaaagaac ctggatattt tctttttgtc agaataaacg gcatcatttg
    23221 caacatcggt tgaatgatca ttcatgctgt ccctgattct ttgaaaatct acgatattat
    23281 ccttcatatt gttatgacct caattttcag gtgggtacat tttatcaaac atattagtaa
    23341 actctgagtc ttccctggta tcgccctcat aaccaattgt ataagagaac tttaatttat
    23401 aaacttttct cccaattttt tctgaaacta cgaaacttaa gttctttact tccgtatgtt
    23461 tcatgatttc ttttactgat ttattaagaa catctctttt aaaagcagga taatctggat
    23521 acttataatc cttcaccccc cccgccccta ttgtataaag attcaattca tctttaagtt
    23581 catctattgt catttcaaaa ctattcgcgc gagaattatt actatatatt ttacgaatga
    23641 gttgatatag tgatgcagca ttaacacttg tcagtcgaac aacagataat aaaacttgcg
    23701 ttgtataacg tttttcttca ccaataagtt tacataaaaa tcttttcgct tctttcgtaa
    23761 acttgatccc cactctacct tcattcctgt aataagtaga aaaaacagtc agactcaaat
    23821 tcattgaatc aggtacatta cttttagtga acttaaatcc caaatcacta ctcagttcta
    23881 atatttcatc atgtttcagc ttaagaagcg tcgtatctaa tatattagag ccatccctca
    23941 aagcaagata agccgcatca ggcttaacct gaacccattt aatgtaatcg gcaactgtca
    24001 cataaaatat gtgatcatca tcgaattcat ttttagagtt tatctggcac atggctaaaa
    24061 ataagacttt tttagcagcc ataggcaatg ttgacagcgt tgaattcaat tcatttcgat
    24121 gtctcacttt tgttaagcga gaaagggaag tcttatttgt cgtcattctc attgttccaa
    24181 cactcttgat aatcagactt taacaccaga aacatgatat tacaacacaa tcaatatcat
    24241 gtttaaccga gtatcttctc atgtttcata tctgtgattt atctcacaaa ggtaatccat
    24301 gctcaaaatc cacactggat tcaaggcaga ttaacaacag attcaaaata gattcaccag
    24361 aaagaacaac gaattagtat tcttatgaca cattttgtgg atatcaccga ggaagctctc
    24421 atgtttaacc gaggaagctc tcatgtttaa ccgaggaagc tctcatgttt aaccgaggaa
    24481 gctctcatgt ttaaccgagg aagttatcat gtttaaccga ggaagctctc atgtttagac
    24541 aaaaaacacg cttaaaatca ataaattaga gctactctaa gtttttaagt ttttaagaaa
    24601 aacaagaaaa gataaggctt ttaatgcgtg cgaatattca ggaactgtgg ataactcaac
    24661 aaaaccagca acgatgatgg ttccttacca taaatctgtc ccctgatgat tattacccgc
    24721 aagctaaaac aaacatcatc aatcattaaa actcataact ttcaataaca taaaatcatc
    24781 aaaaaaatat ttgatgcctt atgtatctca attttcaaga ccataaattt gtccctagtt
    24841 aactttaact tattgaaata aatataatat tcgtgcgtac tgatcctaat gtcacatcgc
    24901 aaaaattccg cttcctcgct cactcagtcg ctacgctcct gccgttcggc tgcggcgagc
    24961 ggtatctcct ttctcaaaac cagagagatt cagatcatat gatccgttat ttgatccttt
    25021 ttggatcttc cactaagaag ccctgaatcg cgctctgccg cttcgttcga tacaaccatg
    25081 aggaaaacca tgcgaaatga aaaaaatcgc ttaaaatgcg attcagagcg ttttagggag
    25141 gttctgttag ttctcctgca ttgcgcccgt agttcgctaa cgctcacacg cgcagtacag
    25201 cgccccgcca tgctgcaatg caccagggcg gggtaaactc gcttgcgctc gcgctaactg
    25261 cggcattctg cggcgcaaat cagcataatc atggcctgct ggtcagttgg gctttgcagg
    25321 gtgaagggat ggcctgtgca ccctggtacg ttcgtcagcg gctgcgccgc gcttcggggc
    25381 gatgtggctc ctgtgctgcc ctggtatttt cgttcgcccc ttccatccgc ttacgcggat
    25441 taaaaaacgt gataacaatc acttatcgag taaaaaaacg ggagaaaact tgcaaatacg
    25501 acttaaaggt cgtattattg tttcatcgaa aggggaatgg cccctctcac aatagcccag
    25561 cagggcagga gattaaaaaa atgaatatcc aggaagcatt aaacgttttt ggattatccg
    25621 gcgaattaac tgaaaaagat atcaaagcag catacagaaa agccgcttta aaatatcatc
    25681 cagatcgtaa cccgttaggg gctgaactga tgaaagcggt aaatgcagct tttgatgtat
    25741 tgatggcaaa tattgataaa ataaatcagt tccagagcac tgatgaacat gcacgatata
    25801 attacggtga tgacctggaa aaagtattaa acgttctttc tggtttatct ggtctggtat
    25861 ttgaagtgat aggtaactgg gtctggatta gtggagaaac cattacacat aaggaaactt
    25921 taaaagaaat cgggtgtaaa tgggcggcaa agaaaaaaca atggttttat cgtccagacg
    25981 aacataaaag ttactggaat cgtgaagaac acacgataga agaaatccgc gcaaaatacg
    26041 gtacaaccgg acagcgcagg gcgacagggt ggcaacgcgt ggaaaccaga gcgtaaccag
    26101 aacgggggcg agaagccccc gatattatag ccagcccgtc ttttggggca tcgaacccaa
    26161 aaagacggga acagcagcca cgtagcaaaa agggaaacag atgaactctt tttttgaaca
    26221 atatcaccct gtctttgaag ttgtctgccg tatcctgggg aacggctggc gcgtgaacaa
    26281 acttgatgat tgttcgtctc gtataaaact gacgtcaccg cagtttaaaa attactctgt
    26341 gcatattcgg atggaaaaag accgcttttc tgtggtgggg agtgtggata gtcgttcctg
    26401 gcgtagtccg catcatgttt gcacgttatc cagaaaacgg aatcccgttg atattgcagc
    26461 cgatatcgag cgaaaaattc tggtaaatgc ttcgcaggag gtgttacagg cgattgagta
    26521 tgagaaacac caggtggaaa aaaaggacga aattctgatc ctgaaaggta tgttatcgca
    26581 acttgtccag cttgaaagct ggtatggggc attgacaggc tttaaagctg aaaacggatt
    26641 gaacggtaaa gtcaccgaac agggcgacag ttatgattta cagattcggg gcttgagtat
    26701 agatcaactt gttaaaatta cagggtattt gaaacagtta tgagaaaaaa taaaggtgat
    26761 gtgacttatt ttcttgaaaa agaaggtgac aattacaggc ttacaaaaag gataaaagcc
    26821 agaacaaatg ttaaaatcgg gaacaaaact acgaaaatta cattgtatga tgctgtactg
    26881 aatgagaatg aattacaaca tattgatttt acgtgtgccg gattaaggga agacgatgag
    26941 acgccagtta agaatttaat taaggagttt atgttaaatg aaacccgata atacacctga
    27001 aaatgtaaaa aaattacgtt taaaagcagg attaacacaa aaggaatgca gtcatattta
    27061 tggtgttggg ctaagaacct ggcagaaaaa agaggaagtt aatactcaaa acagtcaaag
    27121 tctgtcactg gttgagtttg agtttctttt gttacttgca ggagaacatc ctgaatacgt
    27181 gctctgcaaa agggagtcaa aataaaatga cctgtgatgg ttttatatta gtacctggag
    27241 ttgcgctcgt agctcgctga cgctcacacg cgcagtacag cgccccgcca tgctgcaatg
    27301 caccagggcg gggtaaactc gcttgcgctc gcgctaactg cggcattctg cggcgcaaat
    27361 cagcataatc atggcctgct ggtcagttgg gctttgcagg gtgaagggat ggcctgtgca
    27421 ccctggtacg ttcgtcagcg gctgcgccgc gcttcggggc gatgtggctc ctgtgctgcc
    27481 ctggtatttt cgttcgcccc ttccatccgc ttacgcggat taaaaaacgt gataacaatc
    27541 acttatcgag taaaaaaacg ggagaaaact tgcaaatacg acttaaaggt cgtattattg
    27601 tttcatcgaa aggggaatgg cccctctcac aatagcccag cagggcagga gatttaaaat
    27661 gaaatgctca tctgttttta cttcaaccac taatcatgtt ttcacattcg aacgagtaac
    27721 gctttgcact attatcctta tgcataagga tactggacaa cagtatgttg tcatatttac
    27781 tgataacaat aaaattcgtg actataaagc cggaattgtt cctcagtttg gcgaacttaa
    27841 gcagagtgat gttgatttag tccttttcta cagggacgaa tatgaaaaat attttgattc
    27901 attaaaagat ggcgatgaat gcttgagttt taaagatttt attgagtgtc tttgttaagc
    27961 attatgaatg caggggcatc cctgcatttt ctggaaattt caatattgat tctgaaattg
    28021 tctattaaca tcaatgcaag ggaaattcat gaaaacgtta aaacaagccg caatgcagtt
    28081 tgcatctgat ctgcgtaata atcgctgttt taaagctgct tataatgatg ctctgttaga
    28141 gcttgatcgg gaagatttaa acgccattgt acaatgcaca tttctttttg agccaaaacg
    28201 aacattagcc gaacttgata aattaattaa aaagatagaa gtagaagaag attatgagtt
    28261 ttgtgtatcc cttgataaag gtaaaggatt acacgaagtc agaacattta aaagtgagcg
    28321 tgaggcgttg gatgcttatc gcattttgac tctttacgga tacacagtaa ctatagaaaa
    28381 gaaggagggt aatgactgac tattcaaggt gtgatcaggt tgttccacca acctgatcac
    28441 gttatcgaca atcaaaactg aatgaggttt ttaaatgtca acgcggcaga tcgtacagct
    28501 tgctgattcc cttgtcaaag gctgggaaac cagaattccg gcaatgaaat ttagcgaatg
    28561 ggatcaattc cggtggtggt taaactactt gcaagggtat gaaatgtttt aaataaaaaa
    28621 aggggctttc tgcccctttg tattttttga caaaaatgcc atgtgtaact ataattagtt
    28681 gcatatctat aggggatgga tttatgggga aaacagataa gctactggca aagtttttaa
    28741 acagtaaaaa aacgtttgaa tgggatgaac tggtcgtttt gttttcctct ttgggatatg
    28801 tcaaaaagga aatgcagggg tcaagagtgc ggtttttcaa tgctgaaatc aatcacacca
    28861 tattaatgca tcgtccacat ccggaaagtt atatcaaagg tggtacgctg aaagcaatta
    28921 aacagaatct gaaagaggct ggattattat gaaacattta aaatataaag gatatttagg
    28981 cacggttgaa cccgattttg aaaataatgt cctgtatgga aaactggcgt ttattcggga
    29041 cctggtaact tatgaggctt ctacattagc tgaactggaa caggagttta aaacatctgt
    29101 tgatctgtat ttacagtctt gtgtggagga tggaaaggaa cctgataccc cgtttaaagg
    29161 cgtgtttaac gtcaggcttg atccagaact gcatcgtcgg gtagcagaaa tggcgatgga
    29221 agaagattta tcgttgaatg cctttgttaa taaggctctg gaaaaagaag tcagcaatca
    29281 tcgcgcaggg gcttaattgc ccttgttttt atgccctgta agggcatggg aggcgctttg
    29341 cgccggaagc cccgcagcgc agcgaggact taaaaagcgt tacggcttca gccgtaacaa
    29401 ttcccagccc tgccaggcct gttttttctt tcacaaatct ttcagctata cgtttgcctg
    29461 atggtcgaag acgaaagaat ctttcagcaa cgaaatctgg acatatgcag cgacgatttt
    29521 atagccaggc acagtgccac atatggtaca atcaatgcag gatagacaat ccgatgtcaa
    29581 atgttaacat ttgaatcaga ttgtccaaaa acgatgtttt tgcctgtgcc tgccgcaggc
    29641 tctgttgggg tgacttatga agatgatagc gttcaggcta agtgatgatg agttaaaatt
    29701 tgcagaacat aacgctattt taagtggatt taccagtatc aatgcttttg ctaagcataa
    29761 cgtgcttaat attgaaacca agcctgttaa catcccagtt aataatgaac cagcaaagct
    29821 ggtatcagtg cgtctttacc ctcatgaaat agaacttgtt aaacgtaatg ctgctcttca
    29881 cggcatgagc atgagcagag aaattgctat tcgtgtgcgt cagtctttgt taaagagtga
    29941 agtctgtctt taccctgatg aagttaagga acttaaaaaa ctatcaactg ctgtagatcg
    30001 tgttggtcgc aacattcatt ttatcataaa aggtgaaaga ttctgtacgg ttaacgatcc
    30061 agatttcaga aaagacgtta ttgaagtgat tgaactgtgt aagcaaattg attcaaaact
    30121 tgaaacgtta accaagagtg ttgttaacag atttgggtga tttatgggtg tgtatgtaga
    30181 acaggaatac cgtattaaac gcgctaaagg gactgggcgt gatccgaaat ctcctaagtt
    30241 atcgggaaga catatacatg ccagtaaatc ggcttttaat cataaagtca gacatggtgc
    30301 ggacaagaaa gcatatacct ggacacctac aaaagaagtc acttttaaaa tcactggctc
    30361 aggtaaaaca gccgtaggca tcaaaaatgg tattgattac attacccgta atggtgaact
    30421 tgaggcttac tgttatgatg gaaaaggttc agagcaaaca gggaaaggag aagactttaa
    30481 tagggagttt acttcaactt tatctaaagg aaatgattac tccaggacat atcgtggaga
    30541 aaacatcgat catgtgaaaa acatggtctt ttcaccacca ccagaagctg gagtaagcag
    30601 ggaagacgct ctgaaagcta cggtagaatt tcttaaagag acttatccaa accatgcttt
    30661 cgttgccgta taccatgatg ataaagaaga tcatcctcat gtacatgtta atattaaact
    30721 tcgtgatgaa gaaacaggaa gaagattaag acttactaaa tctgaaacca ggaagttcag
    30781 aaacggtttt catagaaaac tgaaaggtat gggatatgat gttaccgcta cctggaaaaa
    30841 agatcccgaa cgtaaacggg agattgagcg attacaggct gaaaatccga aaagactccg
    30901 gaatgtttac aaggtggtgg attttgggga gacttcatat cagaataagg caggggaaaa
    30961 acgaacacct ttcctcactt atgaaacttt aaaaggtgga aaacaggtta cgatttgggg
    31021 taaagacctg aaaaatcatt tcgagtctga aaagctccag ccaggaacgc ttatcaaagt
    31081 aaaaaaactg gctcctacgc ttgtacgtag tccaatgttt aatgatgatg gaactgttgc
    31141 aggctacagg gaaacgcatc gcaataactg gcagattgaa aatattgccc ttgagcggaa
    31201 cagagaacgt caggttcatg agcgtgaaac caggcagccg gaagagagcg acgttaaaaa
    31261 gcagctatcc agaaaacatg agcaaggtca caatatcggg tttgctcttg aacacgggtt
    31321 tacaaaggat tcagaagaac ataaaaaact aagaatacag caagagagga actggaaagg
    31381 cctgggattt taatttgatt ttggcgcagg tgaacaaagc ggctactttg ctcacctgct
    31441 gatcacaaca acctacgagg gaggttatca tgactgagca tgatgctatc tgcatttctg
    31501 gacttcatca aatttttagt gatgaagaac atttgagtga gcaacaaaaa gacattattc
    31561 tgatgtacgc ttatggctat accctgaatg aaattgctga tttcaaagga ctaaagcctt
    31621 caactgtcag aaaatatctc gattctgtaa gagctgaact gggaggtgtc tcgttggcag
    31681 ggatcagaac actggtactc attcggacta atgcccttct cgtgtcaagt ctttccagga
    31741 tcagtgagag ggggaattta tgaaaaaata tctgcttttt gctttgccgt tcttcgttgt
    31801 tggttgtagt gaagaagtaa aaagtgtgga ttggtggggg cagcatctta cggaagctaa
    31861 acaaaagcag gcagagtgtg agaagtctgg ctctgattct cagaactgta agaatgttaa
    31921 acaagcattg tttattcagt ctcagaagga tgctccagtc cctacattcg attaactaat
    31981 gtttcaatca tactagagcg cccatatggg cgcttttttt tgcatgatca cacgaatcat
    32041 aatatgacca ttttgtcaat gttatttgat cgtattgttg agcattgtta ttttgcgaat
    32101 catgttcgca tctaaaacaa atccaaaaca cacttatagt ggattgatta aatgaaggag
    32161 aaatcagata agtcatcgga aaaaggggtt tatgtcacga taaaactcga tcccaggttg
    32221 aatgcattta tcgaagagaa aacgaaaaca tctttgctta gtaagaggaa gcaaattatt
    32281 tacttattaa tgcagagtat gcgtattgat ggatatatgg agcgttagct atggcgtctt
    32341 tattcccagc cttgttgctg tgtgcggcgt cagttcatcc ggatacgata aatgatattg
    32401 cacgggtcga gagtggcttc aatccttatg ctgtggctga aattattcct gaaagggaac
    32461 gcggtagcta tggcaaaagt gttatcagtc atatgcccaa aagtaaggag gaagctctaa
    32521 cagttatcag agaaattgaa aacagaaaaa gacgttattc tgtcggattg atgcagatta
    32581 ccagcagtaa tttttctttt tattccacgt ccgcagaaaa actccttgat tcgtgtgaaa
    32641 acttgtctgt tttcgaaaag attatcgtgg attgttataa acgagggagg agcctggaga
    32701 atgcgcttag ttgttactat acaggaaatt ttagtaacgg taagcgaaaa gaaaaagaat
    32761 ttaataatac aagttatgtt gagcgaattg gttatacagg gaacgaaaaa aaatatgttg
    32821 taccaggtac aagaagcaac gggggagaac agcgaaaaaa tcgttcacac aatgcgtcag
    32881 ttatctggcc cgaaaccatt ctgaaatctg cttttgttga taactcacat ccaacaaaag
    32941 ttattaatta acaggactta taaaatgtca tttaagaaat cagtatgtaa tcgtatcagt
    33001 tcatctgtca ctgctgtaat gcagcgtaaa aataaggtag ttatggcagt cgccggggca
    33061 acagttgcat caccggcctt tgcggatgga ttttcaaaag cggaaacact gctggaaaag
    33121 gttaaaacag ggcttagtgg cctttcactg gtcacggtga ctattgcctg tctgtgggtc
    33181 ggctacaagg ttctgtttgg tggaagcacg attcgtgaat gctcacctat catcatcggg
    33241 gcaattgtta tcgccagtgc ggctgaagtt gcatcaatga tggttaacta ataaagcggg
    33301 gacgatagt
//
