LOCUS       NZ_CP037741             1916 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter freundii strain CAV1857 plasmid pCAV1857-1916, complete
            sequence.
ACCESSION   NZ_CP037741
VERSION     NZ_CP037741.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11055879
            Assembly: GCF_004344985.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Barry,K., Wailan,A., Sheppard,A., Crook,D., Vegesana,K.,
            Stoesser,N., Parikh,H., Sebra,R. and Mathers,A.
  TITLE     Don't overlook the little guy: An evaluation of the frequency of
            small plasmids co-conjugating with larger Carbapenamase gene
            containing plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Barry,K., Wailan,A., Sheppard,A., Crook,D., Vegesana,K.,
            Stoesser,N., Parikh,H., Sebra,R. and Mathers,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2019) School of Medicine, University of Virginia,
            409 Lane Road, Charlottesville, VA 22908, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: HGAP v. 2.3.0; Unicylcer v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 118.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:04:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,343
            CDSs (total)                      :: 5,222
            Genes (coding)                    :: 5,119
            CDSs (with protein)               :: 5,119
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 25 of 103
            Pseudo Genes (incomplete)         :: 72 of 103
            Pseudo Genes (internal stop)      :: 19 of 103
            Pseudo Genes (multiple problems)  :: 13 of 103
            Pseudo Genes (short protein)      :: 1 of 103
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP037741.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="CAV1857"
                     /isolation_source="hospital sink"
                     /db_xref="taxon:546"
                     /plasmid="pCAV1857-1916"
                     /geo_loc_name="USA:Charlottesville"
                     /collection_date="2013-12-12"
     gene            join(1832..1916,1..152)
                     /locus_tag="E1A41_RS27130"
                     /old_locus_tag="E1A41_27130"
     CDS             join(1832..1916,1..152)
                     /locus_tag="E1A41_RS27130"
                     /old_locus_tag="E1A41_27130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031942344.1"
                     /translation="MTRLTNLTPAEKQFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRHADRQRALREEERQQAEFTWSRPRSPRR"
                     /name="hypothetical protein"
     gene            complement(408..710)
                     /locus_tag="E1A41_RS27135"
                     /old_locus_tag="E1A41_27135"
     CDS             complement(408..710)
                     /locus_tag="E1A41_RS27135"
                     /old_locus_tag="E1A41_27135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_031942343.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGAHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     gene            complement(1018..1131)
                     /locus_tag="E1A41_RS27145"
                     /old_locus_tag="E1A41_27145"
     CDS             complement(1018..1131)
                     /locus_tag="E1A41_RS27145"
                     /old_locus_tag="E1A41_27145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     Typing          1493..1666
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(1023..1507)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.648"
                     /coverage="80.0"
                     /evalue="0.0"
ORIGIN
        1 aactcaacca gccgaaccga catatcgtgc tcaaccgggc aagagcgcag attgagtcgc
       61 agcggcacgc cgaccggcag cgcgctctac gtgaggaaga acggcagcag gccgaattta
      121 cctggtcacg tccgcgatcg ccgcgccggt gagtggctgt tatgaggttt ctgctgcagt
      181 tttgtgatga aactgcaggc aaggcatttc cgtgcatagc catgcagacg cgcagaaatc
      241 atcaggaacg tttttcgggg ttttccccgg ggaaaggtgc cggaaatcgg catgaggccg
      301 cagaaacgcg ctgagagcgt cttaggcggt accgataagg gatgttatgg taaatgtgcc
      361 tcacagactg gtacaaaaaa agccccgcag ggcttttttg ctatgagtta gccatatttt
      421 cttgcgaatt tttcatcgga atcgcataga taaataataa actcaataaa cgcgatgatc
      481 gcgggtatga atgtccagca aaaaattaga tacaggaatc cctgtcctat tttacctaag
      541 taaaatttat gtgcgccaaa tccaccaagg aagaatgcta atagcgcggc tgcgattcta
      601 cttttttcac ctgaacctga agaggcgttt gtggctccgc agtgtgggca tgactttgct
      661 gtttcgtgta tttccttgcc gcatcctcgg cagtaaacca ttgaactcat tttttatcct
      721 cgagcttatt tttagttggg atgcataata tccaatgttt tataacgata tgcatcctat
      781 atgctcgggt gggatagatt ccattatggt gaaggatctt cttgagatcc tttctgtagc
      841 gtcgtaatct cttgctctgg aaacgaaaaa accgccctgc aaggcggttt ttcgaaggtt
      901 ctctgagcta ctaactcttt gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa
      961 cttgtccttt cagtttagcc ttaaccggcg cataacttca agactaactc ctctaaatca
     1021 gttaccagtg gctgctgcca gtggcgcttt tgcatgtctt tccgggttgg actcaagacg
     1081 atagttaccg gataaggcgc agcagtcgga ctgaacgggg ggttcgtgca tacagtccag
     1141 cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagaa accgcggcca
     1201 taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagct
     1261 accaggggga aacccttggg atctttatag tcctgtcggg tttcgccgcc actgatttga
     1321 gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gcttcgccgc
     1381 agcctcatcg tttccctgtt aagttccttc ctggcatcca ccaggaaatt tccacctcac
     1441 ccgtaagccg gtaccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     1501 gaagcggcac atctcccgtt actacatgtt ctgctgacgc gccggtgccg cctttttctc
     1561 ctgctacatg aagcacttca ctgacatccg catccgtgcc aacatattaa gccagtatac
     1621 actccgctag cgctacgtga ctggttcagg gctgcgcccc gaaacccgcc aggaccactg
     1681 gcgcgcctga cggcttgccc aacgcttcac ggcacctgat taaaatccgc tcccgttcac
     1741 cgtttctgat ggttctgaca ttccgccgct gcttcattgc ttccaccttg tctggcagac
     1801 tggccagcgt gatgctgaac aggagaccta catgacgcga ctgaccaatc tgacgccggc
     1861 agagaagcaa tttcttgatg atgctgttgc agctgcagag cgtgcttccg gcaaga
//
