LOCUS       NZ_CP036552             5594 bp    DNA     circular CON 25-JUL-2023
DEFINITION  Bacteroides fragilis strain DCMOUH0042B plasmid pBFO42_2, complete
            sequence.
ACCESSION   NZ_CP036552
VERSION     NZ_CP036552.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02892979
            Assembly: GCF_000724795.2
KEYWORDS    RefSeq.
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Sydenham,T.V., Hasman,H. and Justesen,U.S.
  TITLE     Complete genome assembly of MDR B. fragilis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Sydenham,T.V., Hasman,H. and Justesen,U.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2019) Department of Clinical Microbiology, Odense
            University Hospital, J.B. Winsloews Vej 21,2, Odense C 5000, Denmark
REFERENCE   3  (bases 1 to 5594)
  AUTHORS   Sydenham,T.V., Hasman,H., Soki,J. and Justesen,U.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2014) Department of Clinical Microbiology, Odense
            University Hospital, J.B. Winsloews Vej 21,2, Odense C 5000, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: OUH_BFO42_3.manual
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/25/2023 04:04:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,188
            CDSs (total)                      :: 4,095
            Genes (coding)                    :: 4,045
            CDSs (with protein)               :: 4,045
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 18 of 50
            Pseudo Genes (incomplete)         :: 36 of 50
            Pseudo Genes (internal stop)      :: 15 of 50
            Pseudo Genes (multiple problems)  :: 17 of 50
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036552.1.
            Bacteria and source DNA available from Ulrik S. Justesen.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="DCMOUH0042B"
                     /isolation_source="Blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="pBFO42_2"
                     /geo_loc_name="Denmark: Odense"
                     /lat_lon="55.3958 N 10.3886 E"
                     /collection_date="2008"
                     /collected_by="Ulrik Stenz Justesen"
     gene            join(5389..5594,1..1045)
                     /locus_tag="HR50_RS22685"
                     /old_locus_tag="HR50_022680"
     CDS             join(5389..5594,1..1045)
                     /locus_tag="HR50_RS22685"
                     /old_locus_tag="HR50_022680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBU/pMV158-type mobilization protein"
                     /protein_id="WP_005799237.1"
                     /translation="MAKTCIRVEACNIGSSERHNLRSKELDYIRPELTHRNEQWVECSI
                     AEVHRDITEKYKEATGQGLQKKATPIREGVIVISEETTIQQLQDLAEKLEERFGVHAFQ
                     IYTHKDEGANVWDGKEEAWKPNYHAHMIFDWTDGHTGKTVKLNRHDMAEMQTITAECLN
                     MERGVSSDKKHLSAMQYKNKMETEKAEQLQKDIEQLNRAYTAGTEKITTVQKELSTAQK
                     ELNSMKTDIHINEAKEAAAKAGKTVFNAVTSVFNVGEVKRQAKEIETLKKENYNLSFKN
                     QNLEGQLRTNNIEMRRVQETTDRQIKAEASKLKPITNLFPEMENAQENIEELQTMGIQN
                     KDIRQLLIGKEIYYTGNLYDKDKRKSYQVKNVKIDISKSQNGITTIWLNNIHFKNFLKE
                     LWNTLQKTLDNGNWLHR"
                     /name="NBU/pMV158-type mobilization protein"
     gene            complement(1115..1885)
                     /locus_tag="HR50_RS22905"
     CDS             complement(1115..1885)
                     /locus_tag="HR50_RS22905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005799241.1"
                     /translation="MKKVMFMFISLLTVLGVCSCNDNEVIEPVVSDSVSDMEVLSRFVD
                     VNEITNEYYFNENKKTRALSYVTGSDWQDLEKVSPLSIEKYKNNLQVLNAQVASAISNP
                     NTAYVVFSVNGKTLVKKVKEDANFDFSVFRDVVTETRAVLPSLSINGGSQSTTGVFYDS
                     SRTLKMQVDLNASIQNNYYFFEVLNPNAKPSPDDNITTPESVAFSGTGPLWSNTFTWTS
                     YWDANVPGQGFKWEFKGKGTTPSFGFIANCTFSR"
                     /name="hypothetical protein"
     gene            1977..2438
                     /locus_tag="HR50_RS22695"
                     /old_locus_tag="HR50_022690"
     CDS             1977..2438
                     /locus_tag="HR50_RS22695"
                     /old_locus_tag="HR50_022690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005812785.1"
                     /translation="MNRIIHISLLISTVSLFLISNTGCVEKQGYYNHGEESIISLICDI
                     TWAGKKTTDENGSVWQGTYKFNKNGTYTRTNIEIDKQGNKKEANIYGQWSFGDPSFSTI
                     YFGGEHYWDIDELTKNKFSFYDRSGKFGDPFMNREYIELTPYQENNTTN"
                     /name="hypothetical protein"
     gene            complement(2442..3344)
                     /locus_tag="HR50_RS22700"
                     /old_locus_tag="HR50_022695"
     CDS             complement(2442..3344)
                     /locus_tag="HR50_RS22700"
                     /old_locus_tag="HR50_022695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_225538865.1"
                     /translation="MKAYMDAHVCSNVYTVQGGKAHSHYCHRAFCPICQRIQTAQNVKK
                     FLPVMKYLASEGREFYFVTLTLQNCVTDDARVLRDFMRRCNRMWADGIRTKHKFRSLGI
                     SGVIKKECTYHVVKKDLFSFHYHFHIIVDSLEAAKYVVAQWKKLHGSTVADARFQKYKK
                     IEDFENAAIEVFKYASKASVSKSKGRDGKKKIQINYKALDMIYTAMHGMQRMSSFGQFR
                     TMIGDEALNDFRDEDLVLNIEGLNVEDGCYTWYMSVKDKVADWYNMETGEALCCYKVTR
                     KDELLQDIYLDEDIFPNSG"
                     /name="protein rep"
     gene            complement(3986..4384)
                     /locus_tag="HR50_RS22710"
                     /old_locus_tag="HR50_022705"
     CDS             complement(3986..4384)
                     /locus_tag="HR50_RS22710"
                     /old_locus_tag="HR50_022705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005799260.1"
                     /translation="MKPTDYIEWDNLKDIPFFLCQVVEDREKQDLDIYYLGKRVLHDYD
                     HVGHYLRTAVILFRRVKSRTADWVNLRNLWTLRNCVRENYNHGIGMNDLIFGENFDGDN
                     LDTLTPLTKKRFDFLCKRIKELDPYATI"
                     /name="hypothetical protein"
     gene            complement(4421..4741)
                     /locus_tag="HR50_RS22715"
                     /old_locus_tag="HR50_022710"
     CDS             complement(4421..4741)
                     /locus_tag="HR50_RS22715"
                     /old_locus_tag="HR50_022710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799262.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003723 - RNA binding [Evidence IEA]; GO:0004521 - RNA
                     endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_005799262.1"
                     /translation="MVEQYEVYWVELDPTRGGEMAKTRPCVVVTPSDLNMYLTTVVIVP
                     ITSTIRNYPYRVLCSVAGREGEIATDQIRTVDKSRLKRKIGDLNFSEIKRLREVFQQMF
                     CE"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(4735..4983)
                     /locus_tag="HR50_RS22720"
                     /old_locus_tag="HR50_022715"
     CDS             complement(4735..4983)
                     /locus_tag="HR50_RS22720"
                     /old_locus_tag="HR50_022715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_005812783.1"
                     /translation="MVTNIIQIGNSKGIILPSEVLKQLRLSLKSAVSISLDGNNIVIKA
                     QPRQGWAEAAKRAHENGDDELLIPDVFEDEKFEDWTW"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     Typing          complement(2443..3491)
                     /name="rep_cluster_870"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.81"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..1042
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cccaatccgg gaaggggtaa tagtaatcag cgaggaaacc acaatccagc agcttcaaga
       61 cttggccgaa aaactggaag aacgctttgg agtgcacgcc ttccaaatct acacccataa
      121 ggatgaaggc gcaaacgtat gggacggaaa ggaagaagca tggaaaccga actatcatgc
      181 gcacatgatc tttgactgga cggacggcca tacaggaaaa actgtaaagc tgaacagaca
      241 cgacatggcg gagatgcaga ctataacagc agagtgtctc aacatggaac gtggagtttc
      301 ttcagacaag aaacacttgt cggccatgca atacaaaaac aaaatggaaa cagagaaagc
      361 tgaacaactt caaaaagaca tcgagcaact aaacagggca tacactgccg gaaccgagaa
      421 aatcaccaca gtccaaaagg aacttagtac ggcacaaaaa gagctaaaca gcatgaaaac
      481 cgacatacac atcaacgaag ccaaagaagc tgccgcaaaa gccggaaaaa ccgtatttaa
      541 tgccgttaca agcgtcttta atgtagggga agtaaaacga caagccaaag aaattgaaac
      601 cctcaaaaaa gaaaattata acctctcatt caagaatcaa aatttagaag gacaattaag
      661 aaccaacaac atcgagatgc gaagggtaca ggaaacaacc gacagacaga taaaggcaga
      721 agcatccaaa ctaaaaccca taaccaacct ttttccggaa atggaaaatg cacaggaaaa
      781 tatagaggaa ctccaaacta tgggaataca aaataaagat atccgacaac ttcttattgg
      841 aaaggaaata tactacactg gaaaccttta cgataaagac aagagaaaat cataccaagt
      901 taaaaacgtg aaaatagata tctccaaaag tcagaatggt attactacaa tttggctaaa
      961 taacatccac tttaaaaact ttcttaaaga actttggaat acactacaaa agactttaga
     1021 taacggtaac tggctacacc gatagaaaga cactaaaaag aattgcactt ctcttttttg
     1081 agaagtgcaa ttcaaattaa atcccgttca ttttttaacg actaaaggtg caattagcta
     1141 taaaaccaaa agaaggagta gtaccttttc ctttaaactc ccatttaaaa ccttgaccag
     1201 gtacatttgc atcccaatac gaagtccaag tgaaagtgtt actccataac ggtccagttc
     1261 cactaaaagc aacactttca ggtgtcgtta tattatcatc cggacttggt ttagcattag
     1321 gatttaatac ttcaaagaaa taataattat tctgtattga agcattcaaa tcaacttgca
     1381 tctttaaagt acgactggaa tcgtaaaaaa ctccggtagt gctttgagaa cctccattaa
     1441 tactcaaaga agggagaaca gccctcgttt ccgttacaac atccctaaat actgaaaaat
     1501 caaaattagc atcctccttc acctttttaa ccaaagtctt tccatttaca gaaaaaacaa
     1561 cataagcggt attaggattt gaaatagcac ttgccacttg agcatttaaa acctgcaggt
     1621 tattcttata tttctcaata ctcaaaggac taactttttc taaatcctgc caatccgaac
     1681 ctgttacata agacaacgcc ctcgttttct tattctcatt gaaatagtat tcatttgtaa
     1741 tctcatttac atcaacaaaa cgagataaga cctccatatc agatacagaa tcactaacca
     1801 caggttctat tacttcatta tcattacacg aacaaacacc caaaacagtc agtagactga
     1861 tgaacataaa cattactttt ttcataaatc ctcctttttt aaagttaata aataaaggtt
     1921 tcgcccaaaa atataaaatt tctcctattt ttgcattgaa tattaaatat ttaactatga
     1981 atagaataat acatatctct ttacttattt caacagtatc actttttctt atttcaaaca
     2041 ccggatgtgt tgaaaaacaa ggatactaca accatggaga agaaagcata atctcattaa
     2101 tttgtgatat tacatgggct ggtaaaaaaa caacagacga aaacggcagt gtctggcaag
     2161 gtacatacaa attcaataaa aacggaacat atacccgtac aaatatagaa atcgacaaac
     2221 aaggaaataa aaaagaagcc aatatctacg gacaatggtc attcggtgat ccaagtttca
     2281 gtaccattta ttttggtgga gaacattact gggacataga cgagctaact aaaaacaaat
     2341 tttccttcta tgacagaagt ggaaaatttg gcgatccttt catgaataga gaatacatag
     2401 aactcacacc ataccaagaa aacaatacaa caaactaaca actacccact attaggaaaa
     2461 atatcctcat ctaaataaat atcctgcaaa agttcatcct tccgggtgac cttataacaa
     2521 cacaatgcct ccccagtttc catattatac caatcagcca ccttatcttt cacagacata
     2581 taccaagtat aacaaccatc ttcaacattc aatccctcaa tattaagaac caaatcctca
     2641 tctctaaaat cattcaacgc ttcatctccg atcatggtgc ggaactgtcc gaaactactc
     2701 atgcgctgca tcccatgcat ggcagtataa atcatatcca aagccttata atttatctga
     2761 atcttttttt taccgtcacg acctttcgat tttgagacag aagcctttga agcatactta
     2821 aaaacctcta tcgctgcatt ttcaaaatcc tctatcttct tatatttctg aaaacgggca
     2881 tcagcaaccg tactaccatg caatttcttc cattgggcta ctacatattt tgcagcttcc
     2941 aatgaatcaa caataatatg aaaatgataa tggaacgaaa acaaatcctt ttttactaca
     3001 tgataggtac actccttctt aatcaccccc gaaataccaa gtgaacgaaa cttgtgtttc
     3061 gtccttatac catcagccca catccggtta catctacgca tgaaatctcg caataccctt
     3121 gcatcatcgg ttacacaatt ttgcaaggta agcgtcacaa aataaaactc tctgccttcc
     3181 gaagccaaat acttcataac cggaaggaac ttctttacat tttgtgccgt ttgaatacgc
     3241 tgacagatag gacaaaaagc ccgatgacaa taatgggaat gcgccttacc tccctgcaca
     3301 gtataaacat ttgaacacac atgggcatcc atatatgctt tcatcagctt actatccgga
     3361 ttcttttccg aaagttttcg agtaaaatta tgggatatga tttttacttt agcctttttt
     3421 ttaagactat cagaaccgtt ttttaacgga ctttctttgc agttggttat atttgttcta
     3481 tctttgcaca tatgaaattt tgatgtggct ttcgtttggt atccgccccg ccaagaacga
     3541 acacaaacga ttgctaattc ttaaaataca aagcccgttg gttactgcct tcgggctttc
     3601 gttttttctc agcccaaaag taggtaattc attccaaacc gccaaacatc attcaaaaaa
     3661 cctacatata acaagtcaaa aagcatttac cacgaaactt tctagtttat ctagtatggg
     3721 ggctcgcccc cattcccccg gctgcgcaat tctgacacaa atacactact aaatccacaa
     3781 cagacaaaga tacttccgga aaaaaaacta tctttgccgt acacaaagac aacccttgcc
     3841 gagcgatacg ggagagttgc aaaaaaaaca ccggagtgaa ttgacggata tccgacatct
     3901 ccggtggtat tttttttctt atccccctcc cgtcccccaa agggggagca cacaaagaca
     3961 agccctgcct tcactcaatg gagtgttaaa tagttgcata tgggtcaagt tccttaatcc
     4021 gcttacacaa aaaatcaaag cgtttctttg taagtggtgt aagcgtatcg agattatctc
     4081 catcgaaatt ctccccaaaa ataaggtcgt tcattcctat gccgtgattg tagttctcac
     4141 gcacacagtt gcgcagcgtc caaaggttac gcaggtttac ccagtctgct gtgcggctct
     4201 ttaccctgcg gaacaagata acggcagtcc gcaaatagtg ccccacatgg tcgtaatcgt
     4261 gcaagacacg tttgcccaaa tagtaaatat ccaaatcttg tttttctctg tcctcgacta
     4321 cttgacagag gaagaatgga atatccttca ggttatccca ttcgatgtag tcagttggtt
     4381 tcataatgtc ttatttgcat ttttctttcc gtactttcca ttattcacag aacatttgct
     4441 gaaatacttc acgtaacctt tttatctcac tgaaattcaa atctccaatc tttcttttca
     4501 gccggctttt atccaccgtg cgaatttgat cagtggctat ctctccttca cgcccggcta
     4561 cagaacacag aacccggtaa ggataatttc ttatcgttga ggttatcgga acaatgacta
     4621 ctgttgtaag gtacatattc aaatctgaag gagtgacaac tacacatggc cgtgtctttg
     4681 ccatttcacc cccacgggta gggtctaact ctacccaata cacttcatac tgttctacca
     4741 tgtccaatcc tcaaactttt catcctcaaa cacatcaggg ataagcaact catcatcccc
     4801 attctcatgg gcacgtttgg cagcttccgc ccatccctgc cgtggttgtg cctttatcac
     4861 aatattgttc ccgtccaaag aaatgctcac agccgatttc aaggacaaac gcaactgttt
     4921 caatacttcg gaaggaagta ttatcccctt gctgtttcca atctgaataa tattcgttac
     4981 cataatgtta taactttata attgattatc acgatacaaa tatatgtaaa gttataacaa
     5041 taacaaaagg aaaaacaact tttttccatt ttcaccgaga accctgtcaa tggctgcact
     5101 cgtcgggagt gttggtgcac ccccttgccg tgcagcgctc cccgaccgat gattttttat
     5161 tgtgccttgt gtccttttcc cttttgtccg gcttctgttc ccaagcacat ccggaacaca
     5221 tggcagagcg cttgcaaatc ccgtccggaa ggcttacctt tgcaaaaggt tatagcacac
     5281 tataacccct aaaggggttg tgtgcgaggg ttgtcccctc gaccccgtgc gatgtgacgt
     5341 ttcacgccac atcttctaaa cagacataca cacctaaaac ctaaagatat ggcaaagaca
     5401 tgtatcagag tggaagcctg caatataggc agctcggaac gtcacaactt aagaagcaag
     5461 gagctcgact acatccgtcc ggaactcaca caccgcaacg aacagtgggt agaatgttcc
     5521 attgccgaag tacatcggga cataacggaa aaatacaagg aagccaccgg acaaggatta
     5581 cagaagaaag ctac
//
