LOCUS       NZ_CP036551             8306 bp    DNA     circular CON 25-JUL-2023
DEFINITION  Bacteroides fragilis strain DCMOUH0042B plasmid pBFO42_1, complete
            sequence.
ACCESSION   NZ_CP036551
VERSION     NZ_CP036551.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02892979
            Assembly: GCF_000724795.2
KEYWORDS    RefSeq.
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Sydenham,T.V., Hasman,H. and Justesen,U.S.
  TITLE     Complete genome assembly of MDR B. fragilis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Sydenham,T.V., Hasman,H. and Justesen,U.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2019) Department of Clinical Microbiology, Odense
            University Hospital, J.B. Winsloews Vej 21,2, Odense C 5000, Denmark
REFERENCE   3  (bases 1 to 8306)
  AUTHORS   Sydenham,T.V., Hasman,H., Soki,J. and Justesen,U.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2014) Department of Clinical Microbiology, Odense
            University Hospital, J.B. Winsloews Vej 21,2, Odense C 5000, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: OUH_BFO42_3.manual
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/25/2023 04:04:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,188
            CDSs (total)                      :: 4,095
            Genes (coding)                    :: 4,045
            CDSs (with protein)               :: 4,045
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 18 of 50
            Pseudo Genes (incomplete)         :: 36 of 50
            Pseudo Genes (internal stop)      :: 15 of 50
            Pseudo Genes (multiple problems)  :: 17 of 50
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036551.1.
            Bacteria and source DNA available from Ulrik S. Justesen.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="DCMOUH0042B"
                     /isolation_source="Blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="pBFO42_1"
                     /geo_loc_name="Denmark: Odense"
                     /lat_lon="55.3958 N 10.3886 E"
                     /collection_date="2008"
                     /collected_by="Ulrik Stenz Justesen"
     gene            complement(115..>732)
                     /locus_tag="HR50_RS22625"
                     /old_locus_tag="HR50_022620"
                     /pseudo=""
     CDS             complement(115..>732)
                     /locus_tag="HR50_RS22625"
                     /old_locus_tag="HR50_022620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681476.1"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /name="ribonuclease E/G"
     gene            1002..1259
                     /locus_tag="HR50_RS22630"
                     /old_locus_tag="HR50_022625"
     CDS             1002..1259
                     /locus_tag="HR50_RS22630"
                     /old_locus_tag="HR50_022625"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032528340.1"
                     /translation="MKIGYSELFEEEHDGGGYKSGGFYISPSAGISLPVTKKTSIHVGI
                     GYTYNQAKYEFSYIPGIGSMGRNNKRFNAGGLTLSVGVSF"
                     /name="hypothetical protein"
     gene            complement(1315..2214)
                     /locus_tag="HR50_RS22635"
                     /old_locus_tag="HR50_022630"
     CDS             complement(1315..2214)
                     /locus_tag="HR50_RS22635"
                     /old_locus_tag="HR50_022630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5712 family protein"
                     /protein_id="WP_032528341.1"
                     /translation="MNIKIQGGGSGTYANTGSCTGVTTYLQHEDLERMKNGREVQPFFN
                     NSRDYISAQEVTFKIDNNKAKLSRNDAKFYVITVSPSSRELEKMGKTEKEQAEAMRKYV
                     RDDVMQHYAEGFGKGLNKEDIEYYGKIHFERKGADRYDMHAHIIVSRKDRSNTRKLSPK
                     TNHTGKKNCGNVKGGFDRTDFFRKCETSFDKRTGYDRAPEQTFDYLNTMKNGSPKEIFQ
                     KKEWAERVNHERLEKMKAEWSRDLQEPHQEQGREENQQQGNSISQVPEINQAPQRKKQL
                     EEELDQPRKRSRGFGMGM"
                     /name="DUF5712 family protein"
     gene            complement(2211..2813)
                     /locus_tag="HR50_RS22640"
                     /old_locus_tag="HR50_022635"
     CDS             complement(2211..2813)
                     /locus_tag="HR50_RS22640"
                     /old_locus_tag="HR50_022635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BfmA/BtgA family mobilization protein"
                     /protein_id="WP_032528342.1"
                     /translation="MEKDSKTTVAVERTTFAKLDRLAKANSVSKMEFITHAINYFEKYG
                     INPVEHESPAQEMQKLIKRMDQVFAFLKKQETDLVRPACEALAGASTQITISLSSLLSE
                     EKFRRFLKDNKELLAGIINSSDNNGKAIGKTEKAVRDADSTIRKNQEIIFKRIEASETA
                     QIKAYKHIVSFLDAKGKTGLLDDINKVYEREKERRGL"
                     /name="BfmA/BtgA family mobilization protein"
     gene            3305..3619
                     /locus_tag="HR50_RS22645"
                     /old_locus_tag="HR50_022640"
     CDS             3305..3619
                     /locus_tag="HR50_RS22645"
                     /old_locus_tag="HR50_022640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008782594.1"
                     /translation="MEKEPGKKTGKGGAREGSGRKAVSGMYTKTMRVPTVMEEEVLCLI
                     DMYSNWLKSGKMEKGMARRTTPEQRKRAIRSIQEILEYEKQSQKVHKQEEEDKRQLSLF
                     "
                     /name="hypothetical protein"
     gene            complement(3693..3971)
                     /locus_tag="HR50_RS22650"
                     /old_locus_tag="HR50_022645"
     CDS             complement(3693..3971)
                     /locus_tag="HR50_RS22650"
                     /old_locus_tag="HR50_022645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family toxin"
                     /protein_id="WP_008782593.1"
                     /translation="MREVKTGGQFRKDLKRYRNDNTKLEKLYEIVGFLERCEDVPKEFK
                     PHMLSGDYAGHMECHVENDFLLIWIDDEVVKLVRLGSHSELFGKKKK"
                     /name="type II toxin-antitoxin system YafQ family toxin"
     gene            complement(3968..4216)
                     /locus_tag="HR50_RS22655"
                     /old_locus_tag="HR50_022650"
     CDS             complement(3968..4216)
                     /locus_tag="HR50_RS22655"
                     /old_locus_tag="HR50_022650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system protein"
                     /protein_id="WP_008782592.1"
                     /translation="MEVAIKKLTAFRLDSNLLDMLKSAAKREHRSLNNFVECLLMDAMY
                     TEPNEETKAAIEEARAGKNLKKVDTSSFENFIKSCSE"
                     /name="toxin-antitoxin system protein"
     gene            4695..5699
                     /locus_tag="HR50_RS22660"
                     /old_locus_tag="HR50_022655"
     CDS             4695..5699
                     /locus_tag="HR50_RS22660"
                     /old_locus_tag="HR50_022655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648610.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_008782591.1"
                     /translation="MDKLFIPITPPTEEEIMLIQPNNVTFGQYNISEWQENLLTLISDK
                     LQKHMTREEELPRDLFNMPYVTIRCDEAGGQNNKTKVIKEATDMLSKRFSFRWKHPKID
                     KEVETIGGSILIAIHNIKGTNQIMLTLNPWAIPFLVYYGVGVGGTRYSKGLALGLRGNY
                     TKRLYKIICSQRDRKEYYYSIQQLRKDLEIPEKHSNNDIDRKILKPAKERIKESGSDVW
                     FDYKLFCKYPVKGRKPKADTVIFTINTLHPREEGGEQYEQYSFVYRWVSSAMDHPTNDR
                     AVKAVDKIAELGRLKNVYERCCYYDDKVCAGDMVRKHAENSLLKMLREEFGIK"
                     /name="replication initiation protein"
     gene            5735..5920
                     /locus_tag="HR50_RS22665"
                     /old_locus_tag="HR50_022660"
     CDS             5735..5920
                     /locus_tag="HR50_RS22665"
                     /old_locus_tag="HR50_022660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008782590.1"
                     /translation="MKEGDLILVSAEATGLGEPMEAVIDKIETFMGQTLVTVTYTQPDA
                     LSGFGGCFVDTHITKQ"
                     /name="hypothetical protein"
     gene            5988..6497
                     /locus_tag="HR50_RS22670"
                     /old_locus_tag="HR50_022665"
     CDS             5988..6497
                     /locus_tag="HR50_RS22670"
                     /old_locus_tag="HR50_022665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008782589.1"
                     /translation="MQEKANIQTSTLRVPKNILEEIKIYCRKAGKPVGEWVETAWKFIE
                     KNDFDIYDKETTPFLPVPPDIEKERNQVEALCMLMSEFITAQKQIQLLAPELIAKTAEE
                     KVRAEMKSEEQTKELKVLQEENDRLRNEIKVLQEYKEKAYRELCRVRDEQKTFGKIRVN
                     TELLIK"
                     /name="hypothetical protein"
     gene            complement(6541..7644)
                     /locus_tag="HR50_RS22675"
                     /old_locus_tag="HR50_022670"
     CDS             complement(6541..7644)
                     /locus_tag="HR50_RS22675"
                     /old_locus_tag="HR50_022670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032528339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISBf13 family transposase"
                     /protein_id="WP_032528339.1"
                     /translation="MKTTDFKDLGKVNQILPMMQEHFGKSMNLARIKFMAFMLHALCVV
                     QTVSLHKLAAAMPTSVERDSNLRRIQRFIAKYALNFDLVAQMIYSLLPVKTGLVLSMDR
                     TNWKFGDFNINILMLGVAYKGIAFPLMFSLLPKKGNSNWKERKAIVERFVRLFGSECID
                     SLVADREFVGKDWIGWLNRNHIRYYIRIRQNFWLVKPSTGERIRAWWLFNSLKVGQERF
                     YYKLFLHKGEYVYLAGSRIKNSDGVPELQILICFKRPEKGVDTYKRRWEIETAFRAMKS
                     SGFNIEDTHLRDTERIARLLAMVCIALVWAYLVGEHKDENVKPIKTLKHGRKAKSLVKY
                     GLEEISNVLFRPIYVPKFDVFKFLSCT"
                     /name="IS4-like element ISBf13 family transposase"
     gene            7793..8281
                     /gene="nim"
                     /locus_tag="HR50_RS22680"
                     /old_locus_tag="HR50_022675"
     CDS             7793..8281
                     /gene="nim"
                     /locus_tag="HR50_RS22680"
                     /old_locus_tag="HR50_022675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NimABCDEF family 5-nitroimidazole reductase"
                     /protein_id="WP_032528343.1"
                     /translation="MFREMRRKRQLLPPEESLAILERMTGGTLALHGDNGYPYAVPVSY
                     VYADGKIYFHSAVQGHKMDAIRQHPEVSFCVVEQDRIVPAEFTTYFRSVIVFGKARILT
                     DEVEKRAAMLRLAEKYSSGESGMQDEIDKGFDHLVMVEITVEHMTGKEAIELVRQKGK"
                     /name="nim"
     ARG             7794..8275
                     /name="nimA"
                     /gene="nimA"
                     /gene_name="5-nitroimidazole reductase NimA"
                     /tool="amrfinderplus"
                     /drug_class="NITROIMIDAZOLE"
                     /identity="97.52"
                     /coverage="91.48"
     ARG             7773..8281
                     /name="nimA"
                     /gene="nimA"
                     /gene_name="nitroimidazole reductase"
                     /tool="rgi"
                     /drug_class="nitroimidazole antibiotic"
                     /identity="96.91"
                     /coverage="96.02"
     Typing          complement(1319..2214)
                     /name="MOBB"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gatggttttt atcagatagt aaaaaccggg agaaacagca atccccttgt ttcgctttgc
       61 cgcaatgggc aagagaagca aggggattat tgcgtcaggg gaaccgaatg tccgttattt
      121 gatttcgaac tcctccttca tgtctatttc ttcgttgtgc gcgtcataga atttatattg
      181 caggaaggca aggctctggt tcgggatgat acggataccg aaaccatatc tcatctgcca
      241 cttccgtttg agcgacatga agccttggtt gatataggca gccacataag ggtggatgtg
      301 caatgtaaat ttcttgacct tcaacttatt gaccaggtag tcaattttac tttccagtgt
      361 atctgtaaac agaatggaag gcttgatttt ccctttgccg aaacaggtag ggcagtcttc
      421 gtcggtggct acgtccatag cgggacgcac acgctgacgg gtaatctgca tcagaccgaa
      481 tttgctgagg ggcaggatgt tgtgctttgc acggtctttc tgcatgttct gacacatgcg
      541 ttcgtacagt ttctggcggt tttcggcaag gttcatgtcg ataaaatcga caacgatgat
      601 tcctcccata tctcttaacc gaagctggcg tgccaactca tcggcagcgc ccaagttgac
      661 gtcgagggca ttggcttctt gcccgttggc cgatttcgaa cggttcccgc tgttcacgtc
      721 tactacgtga atcatttata actaccatat ccgtagccca gagtcgttat cgtggttttg
      781 tcaataccgg ttattcattt ggcagcggtg agtatggttt cagtcaatgg catatcaaca
      841 ctgtccatgg cgtagatctc tttcataaca atcttttcat tggaggaggt atagggttgg
      901 gtatttcaac agaaagcgac atacttaaaa catacagtct gcctgttttc gcagatgtac
      961 gttatacact ttgtaactta aaagttaagc catacataga tatgaaaatc ggatactctg
     1021 aactttttga agaagaacat gatggtggag gttataaaag tggaggtttt tatatttccc
     1081 cctctgccgg aatatcactg cctgtcacaa aaaagacttc aattcatgtt ggtataggat
     1141 atacgtataa ccaggcaaaa tatgaattct cttatatacc gggaattggc tccatgggta
     1201 ggaataataa aagatttaat gctggtgggt tgactttatc cgttggggtt tctttttaga
     1261 accaaactta aatacagggg acaaacatcc ctcacgcaag catgtatctg taatttacat
     1321 acccattcca aaaccccgac ttcttttcct gggctggtcg agttcttcct ccagctgctt
     1381 cttccgctgt ggtgcctggt tgatttccgg aacttgggaa atactgtttc cctgttgttg
     1441 gttctcttca cggccttgtt cctggtgcgg ttcctgaaga tcccggctcc attcggcttt
     1501 catcttttca agccgttcat ggttcacacg ttccgcccat tccttcttct ggaagatttc
     1561 tttcggactt ccgttcttca tggtattgag atagtcaaag gtctgttctg gagcccggtc
     1621 ataccctgtg cgcttgtcaa atgaggtttc acactttcgg aaaaaatctg ttctgtcgaa
     1681 accgcccttc acgtttccgc agttcttctt cccggtgtgg ttggtctttg ggcttagctt
     1741 cctggtgttg ctcctgtcct tccggcttac gatgatatgt gcgtgcatgt cgtaccggtc
     1801 ggctcccttc ctctcgaaat ggatctttcc gtaatactcg atatcctctt tgttcaagcc
     1861 ttttccgaac ccttccgcat agtgctgcat cacatcatca cgcacatact ttctcatggc
     1921 ttcagcctgt tccttctctg tcttgcccat cttctccagc tcccggctgg aagggcttac
     1981 cgtgatcaca tagaacttcg catcgttccg gcttaatttt gccttgttgt tgtctatttt
     2041 gaaggttact tcctgtgcgc ttatgtagtc acgtgagtta ttgaagaacg gctgtacttc
     2101 ccggccgttc ttcatacgct ccagatcctc atgctggaga taggtcgtta ctcctgtgca
     2161 gctccctgta ttggcataag ttccggaacc gccaccctga atctttatgt tcatagtccc
     2221 ctcctttcct tttccctctc atagaccttg tttatatcat cgagcagccc ggtctttcct
     2281 tttgcatcga ggaaagaaac aatatgctta taggctttaa tctgtgctgt ttcgctggct
     2341 tctatccgct tgaagataat ctcctggttc ttcctgatag ttgaatctgc atcccgcacc
     2401 gctttttctg tcttgcctat ggccttgccg ttgttgtctg aactgttgat tatcccggca
     2461 agcagctcct tgttgtcctt caggaaccgc ctgaactttt cttccgaaag taaggatgaa
     2521 aggcttatgg ttatctgcgt gctggctccg gccagagctt cacaagccgg acgcacaaga
     2581 tccgtttcct gtttcttcag gaatgcgaac acctggtcca tgcgcttgat aagtttctgc
     2641 atttcctgtg ccgggctttc atgctcgacc gggtttatac cgtacttctc aaagtagttt
     2701 atggcatgag taataaactc catttttgac acactgtttg ccttggccag acggtcaagt
     2761 ttagcaaaag tagtacgttc taccgctacc gttgttttgc tgtctttttc catgatatta
     2821 taaggcttat tataacaaat taatatgcta actaattggt ttacaagata gtatatttat
     2881 tcgttataac caaattatat tagtataatt ttctaaatag ctgatttaca gctaaaaacc
     2941 acgtagggca agaggaaaaa acagggcttg cccggtttcc tcttgctgtt ctatcgaaca
     3001 ccctccccag cgcgaaatca gcaaagggga cggggagaga tagaacctat cccacacacg
     3061 tgaacacaca cgaaaaccgc acacccggta cacaacagaa ttacccaaca aaaccgcaca
     3121 acagaacatg tccggaacac cacaaaaaac tcatcggtcg gggagcgccg cacggcaagg
     3181 gggtgtacca acactcccga cgagtgcagc cgtttgcagt gcaaatcagt gtgcggtttt
     3241 acttggatat ccgatataaa atacctaact ttgattccgt aacgaaatca aacaaaacag
     3301 acctatggaa aaggaaccag ggaaaaagac cgggaaaggt ggagccagag aaggttccgg
     3361 aagaaaggcc gtgtccggca tgtacaccaa gaccatgaga gtgcctacag taatggaaga
     3421 agaagtactc tgcctgatcg acatgtattc gaactggctg aagtccggga aaatggaaaa
     3481 gggtatggca aggcgcacca ctccggaaca gagaaaaaga gctatccggt caatacagga
     3541 gatactcgag tatgagaagc agagccagaa agtacacaaa caggaagaag aggacaaacg
     3601 gcagctgtcc ttgttctgaa caaaagcata aaacaaaagt catcagaact tattttgttt
     3661 tgaagttctg ctgactttct aaaatggttt tgttatttct tcttctttcc aaacaattct
     3721 gaatgactgc caagcctaac cagctttacg acttcatcat caatccatat caaaagaaaa
     3781 tcattttcta cgtgacattc catgtgacct gcataatcac ctgaaagcat atgaggttta
     3841 aactcttttg gaacatcctc acaacgttcc aggaatccta caatttcata aagtttttcc
     3901 agtttggtat tatcattcct gtaccgtttc aggtctttcc ggaactggcc accagtctta
     3961 acctctctca ttcactacag gattttataa agttctcgaa gctgcttgta tcaactttct
     4021 tcaggttttt cccggcacga gcttcttcta tggccgcttt ggtctcttcg tttggttctg
     4081 tgtacatggc atccataaga agacattcta cgaagttgtt taagctccga tgttcacgtt
     4141 tggcagcact cttcagcatg tccagaaggt tgctgtcaag tctgaatgca gttagtttct
     4201 ttattgctac ttccatattg ttatactttt tattgcaaat gtataacaat atatatcaaa
     4261 agcaaagaaa ataaacacaa aattactaat atatacattt tttaacgcgc cagaaaattt
     4321 gcctatccaa acccacctaa ctccctaaag ggagggttct ctttttctcc ttctttgtgc
     4381 ggtttttcag agaggcaagc taggtcactt tcaccctaaa atccgctttc ttgttagaat
     4441 gtctttgcag aatatcttgt aagaatgtgt ttttttaatt acaaaacact atatttcaat
     4501 gtattacaaa taaaacaaac gtatcaaaac cgaccaaatt actaaaacaa acgtatcaaa
     4561 accgaccaaa ttactaaaat cccccctacc aaaaaccgac caaattacta aagtataata
     4621 taaaaacaga aaatcttttt gttatttcgg tttttatatg tagtttcgca aagtaacctc
     4681 aaaaaagcag tcttatggac aaactattta tcccaataac accaccaaca gaagaagaga
     4741 tcatgcttat acagcccaat aacgtaacat tcgggcagta taatatttca gaatggcaag
     4801 aaaacctgct tacccttatc agtgacaagc tgcaaaagca tatgacaagg gaagaagaac
     4861 tgccacgtga tttgtttaat atgccctatg ttactatccg atgcgatgaa gcaggcggac
     4921 aaaacaacaa aacaaaggtc ataaaagaag cgaccgacat gttaagcaaa aggttttctt
     4981 tccgttggaa gcaccccaaa atagacaaag aggttgaaac gattggtggt tctatcctca
     5041 ttgctataca caacataaag ggaacaaacc aaataatgct tacccttaat ccttgggcta
     5101 ttccgttctt agtctattac ggtgttggtg taggtggtac aaggtattcc aaaggacttg
     5161 cgcttgggct tcgtggtaac tacacaaaaa ggctgtataa gataatttgc agccaaagag
     5221 acagaaagga gtattactac tccatacagc agttaaggaa agatttagaa ataccagaaa
     5281 agcactcaaa caatgacata gacagaaaaa tccttaaacc agcaaaagaa cgtatcaagg
     5341 aaagtggctc tgatgtatgg tttgattata agctgttctg caaataccct gtaaagggca
     5401 ggaagcccaa agctgacacg gttattttca ctatcaacac cctgcatcct cgtgaagaag
     5461 gtggagagca gtacgaacaa tattcgtttg tttacagatg ggtaagcagc gcaatggatc
     5521 acccaaccaa cgacagggca gtcaaagctg tggataagat agctgaactc ggcagactta
     5581 aaaacgtgta cgaaaggtgc tgttactacg atgacaaggt atgtgcaggg gatatggtaa
     5641 gaaagcacgc tgaaaattca ctgctcaaaa tgctaagaga agagttcggt ataaaataat
     5701 ttgattttgt gacacaatca aaaaaaccaa agatatgaaa gaaggagatt tgatattggt
     5761 atctgctgaa gccacaggct taggggagcc aatggaggcc gttatagaca agattgaaac
     5821 attcatggga caaacccttg taacagtcac ttacacgcaa ccagacgcct tatccggctt
     5881 cggtggttgt tttgtagata cacacataac aaagcaataa tatatttttt gtagaaagtg
     5941 tatattgata aatatattct ttatattttt gcacataaat cattgatatg caagaaaaag
     6001 caaatataca gacatcaact ctaagagtac ctaaaaacat actcgaagaa ataaaaatat
     6061 attgccgaaa agccggaaag cctgtcggtg aatgggttga aacagcctgg aaattcatag
     6121 agaagaatga ctttgacatc tatgacaagg aaacaacacc atttcttcct gttcctccag
     6181 acatcgagaa ggaaaggaac caggtagaag ccttgtgtat gctcatgagt gaatttataa
     6241 cggcacagaa gcagatacag cttctggctc cagaacttat cgcaaagaca gctgaagaaa
     6301 aggtacgtgc tgaaatgaag tctgaagaac agactaaaga actcaaggtc cttcaggaag
     6361 aaaacgaccg attacgtaac gaaatcaaag ttcttcagga gtataaggaa aaagcatacc
     6421 gggaactttg ccgggtacgt gatgaacaga aaacttttgg aaaaataaga gttaatacag
     6481 aactattaat aaaataacat ttatgaagaa attatttatt ttgattttag tatcatttat
     6541 ctaagtacat gacaaaaatt tgaatacatc aaatttcggg acataaattg gtcgaaaaag
     6601 cacgttggag atttcctcca agccgtactt tactaaagat ttggctttac gtccatgttt
     6661 caatgtcttt ataggcttta cgttttcatc tttatgttcc ccaacgagat acgcccatac
     6721 aagagcaata caaaccattg ccagaagtct tgcaatccgc tctgtgtcgc gcaaatgcgt
     6781 gtcttcgata ttgaatccgg aggatttcat agcccggaat gcggtctcga tctcccatcg
     6841 ccttttataa gtgtcgacac ccttttccgg gcgtttgaag cagatgagga tctgaagttc
     6901 aggcacaccg tcggagttct tgattcggct tccggcaaga taaacgtact cacctttgtg
     6961 caggaaaagc ttgtaataaa atctttcctg ccccactttc agggaattga agagccacca
     7021 tgcacggatt ctttcccctg tggaaggctt gacaagccag aaattctgcc ggatccggat
     7081 ataatatcgt atatgattgc ggttgagcca accgatccaa tccttgccga caaactcccg
     7141 gtcggcaaca agagagtcga tgcattcgga gccgaacagc cggacaaatc gttcaacgat
     7201 tgccttgcgt tccttccaat tggagttccc tttcttaggc agaaggctga acatcaatgg
     7261 gaatgcaatc cccttgtatg ccacgccgag catgaggatg ttgatgttga aatcaccgaa
     7321 cttccagttt gtacggtcca tgctcagcac cagccctgtc ttgacgggaa gcagagagta
     7381 tatcatctgc gccacaaggt caaagttgag ggcgtacttg gctatgaatc tttgaatgcg
     7441 gcgaaggttg gagtcccttt caacagaggt tggcattgcc gccgcgagtt tatggaggct
     7501 tacggtctgt actacacaca gggcatggag catgaaagcc ataaacttta tgcgggccag
     7561 attcatcgat ttcccaaaat gttcttgcat catcggaagg atttggttaa ctttgcccaa
     7621 gtccttaaag tcagttgtct tcatacgaaa aagcgggcaa acttttcttt aaagatactg
     7681 attcttaggg acttttgcaa atataaacaa ctaataatta ggcatataac aacataattt
     7741 atattttgtc atctactaaa acctttgcct tatgttaaat gaaagaaata tgatgttcag
     7801 agaaatgcgg cgcaaacgcc agttgttgcc gcccgaagaa agcttggcga tactggagcg
     7861 catgaccggc ggtacgcttg cccttcatgg cgataacgga tatccgtatg ctgtccccgt
     7921 gagctatgtg tatgccgacg ggaagattta ttttcacagt gccgtgcaag ggcataagat
     7981 ggatgccatc aggcagcatc ccgaagtctc gttttgtgtg gtggagcaag accggatagt
     8041 tcctgccgag tttacaacct atttccggag tgtcattgtc ttcggtaaag cccgtatcct
     8101 gaccgatgag gtagagaagc gtgccgctat gcttcggctg gcagagaagt attcgtccgg
     8161 cgagtcgggt atgcaagacg agatagacaa gggattcgac catctggtaa tggtggagat
     8221 aaccgtcgag cacatgacag gcaaggaggc tatagagctg gtgcggcaga aagggaaata
     8281 acaggtggga cgcttttccg tcaaag
//
