LOCUS       NZ_CP036548             8331 bp    DNA     circular CON 25-JUL-2023
DEFINITION  Bacteroides fragilis strain DCMSKEJBY0001B plasmid pBFS01_2,
            complete sequence.
ACCESSION   NZ_CP036548
VERSION     NZ_CP036548.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02730069
            Assembly: GCF_000710365.2
KEYWORDS    RefSeq.
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Sydenham,T.V., Hasman,H., Wang,M., Soki,J., Nagy,E. and
            Justesen,U.S.
  TITLE     Identification of resistance genes in three multidrug-resistant
            Bacteroides fragilis isolates by whole genome sequencing
  JOURNAL   (in) ? (Ed.); THE 24TH EUROPEAN CONGRESS OF CLINICAL MICROBIOLOGY
            AND INFECTIOUS DISEASES; ECCMID 2014, Barcelona, Spain (2014)
REFERENCE   2  (bases 1 to 8331)
  AUTHORS   Sydenham,T.V., Hasman,H. and Justesen,U.S.
  TITLE     Complete genome assembly of MDR B. fragilis
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8331)
  AUTHORS   Sydenham,T.V., Hasman,H. and Justesen,U.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2019) Department of Clinical Microbiology, Odense
            University Hospital, J.B. Winsloews Vej 21,2, Odense C 5000, Denmark
REFERENCE   4  (bases 1 to 8331)
  AUTHORS   Sydenham,T.V., Hasman,H., Wang,M. and Justesen,U.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2014) Department of Clinical Microbiology, Odense
            University Hospital, J.B. Winsloews Vej 21,2, Odense C 5000, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: OUH_S01_3.manual
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/25/2023 04:12:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,504
            CDSs (total)                      :: 4,411
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 23 of 64
            Pseudo Genes (incomplete)         :: 41 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 13 of 64
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036548.1.
            Bacteria and source DNA available from Ulrik S. Justesen.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="DCMSKEJBY0001B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="pBFS01_2"
                     /geo_loc_name="Denmark: Aarhus"
                     /lat_lon="56.1917 N 10.1722 E"
                     /collection_date="2013-08-13"
                     /collected_by="Mikala Wang"
     gene            1..981
                     /locus_tag="EC80_RS23490"
                     /old_locus_tag="EC80_023495"
     CDS             1..981
                     /locus_tag="EC80_RS23490"
                     /old_locus_tag="EC80_023495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812759.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_032536519.1"
                     /translation="MAIVNKDLIQSYIMTTAKYDFNVYEKRIIYRLVEMAQGELQGIKF
                     ADNCQKIEHDLFSCVNITMPIACLLNGEDDKNYARVKEALQSLQRKIFEYEDDEIWQSI
                     SIIALPYIRKRSSVLSFQVHPRVWDCILDFSKGFRKYELKAAMSFKSQFSMRFYELLSN
                     QKTPLIYSIEQLKEMFCVADKYERINDFMRKVVEAAKKELDEVSPYTFEYTPLKTGRKI
                     TAIKFYPVYQPEHRDSDLEKHDLQKQTALSWSLAPEVRSYLKNSIEFSDKEIKNNLDLF
                     ISAQTLLPDLLNELAILKGKSREKKNPKGYIINAIKGKIKDRKKL"
                     /name="replication initiation protein"
     gene            1010..1210
                     /locus_tag="EC80_RS23495"
                     /old_locus_tag="EC80_023500"
     CDS             1010..1210
                     /locus_tag="EC80_RS23495"
                     /old_locus_tag="EC80_023500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005812761.1"
                     /translation="MKEGDLILVSAEATGLGKPMEAVIDKIETFMGQTLVTVTYTQPDA
                     LSGFGGCFVDSHITLSEEKAK"
                     /name="hypothetical protein"
     gene            1324..1866
                     /locus_tag="EC80_RS23500"
                     /old_locus_tag="EC80_023505"
     CDS             1324..1866
                     /locus_tag="EC80_RS23500"
                     /old_locus_tag="EC80_023505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BfmA/BtgA family mobilization protein"
                     /protein_id="WP_005812766.1"
                     /translation="MTDKVTIQPKSKPEKTTISISGETSERLEIYCRANGILRKEFVSL
                     ALEYFERTGFDLKSNALDYSPLEKIIGELNEVKATMQTSNEGTEAIRQLLQAVREQTAK
                     QLPAPELIAKAAEEKVRAEMKSEEQVKELQVLQEENDRLRNEIKALQEYKEKAHRELCR
                     VRDEQKTFGKIKVNTEL"
                     /name="BfmA/BtgA family mobilization protein"
     gene            1877..2407
                     /locus_tag="EC80_RS23505"
                     /old_locus_tag="EC80_023510"
     CDS             1877..2407
                     /locus_tag="EC80_RS23505"
                     /old_locus_tag="EC80_023510"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005812769.1"
                     /translation="MKKNEFRKELVLILVLLCTTIAYAQKNDSSFMVAPADSTKDISLT
                     QRVDSLEHELSYIKLSYELYTLNNDLSIFNNDIGVRTNAIQINIYTHNFDYKLYKAYKD
                     NYEATKDKLLSYQDLIETKKTYFWLKIITYPYTEVEKKLLLSSYMTIDKGYSQLESSLK
                     LMKICLDMYKDSL"
                     /name="hypothetical protein"
     gene            <2610..2831
                     /locus_tag="EC80_RS23515"
                     /old_locus_tag="EC80_023520"
                     /pseudo=""
     CDS             <2610..2831
                     /locus_tag="EC80_RS23515"
                     /old_locus_tag="EC80_023520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(3359..4216)
                     /locus_tag="EC80_RS23520"
                     /old_locus_tag="EC80_023525"
     CDS             complement(3359..4216)
                     /locus_tag="EC80_RS23520"
                     /old_locus_tag="EC80_023525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491447.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISBf6 family transposase"
                     /protein_id="WP_032491447.1"
                     /translation="MVTKLIYSESPMYTMLDKDTIKNEILPHLSKAKRGYATQSCLIEV
                     VNAILYKLKTGCQWAFLPVEALFSGKVLSYGAVYHHYNKWSKSGEWKSMWVRLLDKHRH
                     KLDMSSVDLDGSHTAALRGGEEVGYQGRKKRKTTNALYLTDRQGLPIAMSSPKSGEHHD
                     VHDIEKVIGDMFNDLEKSHIRVDGLFLNADAGFDCDVLRGFLGEKEIIANICINKRREN
                     ADDIFVDDELYAERYSIERTNAWMDSFRTLLNRFDTTVSSWMSWNYLAFAVLLLKKISR
                     KQKV"
                     /name="IS5-like element ISBf6 family transposase"
     gene            4327..4839
                     /locus_tag="EC80_RS23525"
                     /old_locus_tag="EC80_023530"
     CDS             4327..4839
                     /locus_tag="EC80_RS23525"
                     /old_locus_tag="EC80_023530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-nitroimidazole reductase NimE"
                     /protein_id="WP_005812773.1"
                     /translation="MFREMRRKRQLLPQEESVAILEKMTNGTLALHGDNGYPYAVPLSY
                     FYADGKIYFHCAKIGHKVDAIMQNNKVSFCVVEQDNIKPAEFTTYFRSVIVFGKAYILT
                     DETEKRMAMTLLVNKYSFGEPGLSDEIAKSINHLLMVKIDIEHMTGKEAIDLVREKEKF
                     CTSEKTS"
                     /name="5-nitroimidazole reductase NimE"
     gene            complement(5363..6262)
                     /locus_tag="EC80_RS23535"
                     /old_locus_tag="EC80_023540"
     CDS             complement(5363..6262)
                     /locus_tag="EC80_RS23535"
                     /old_locus_tag="EC80_023540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5712 family protein"
                     /protein_id="WP_005812749.1"
                     /translation="MNIKIQGGGSGTYANTGSCTGVTTYLQHEDLERMKNGQEVQPFFN
                     NSRDYISAQEVTFKIDNNKAKLSRNDAKFYVITVSPSSRELEKMGKTEKEQAEAMRKYV
                     RDDVMQHYAEGFGKGLNKEDIEYYGKIHFERKGADRYDMHAHIIVSRKDRSNTRKLSPK
                     TNHTGKKNCGNVKGGFDRTDFFRKCETSFDKRTGYDRAPEQTFDYLNTMKNGSPKEIFQ
                     KKEWAERVNHERLEKMKAEWSRDLQELHQEQGREESQQQGNSISQVPDINQAPQRKKQQ
                     EEEIDQPRKRSRGFGMGM"
                     /name="DUF5712 family protein"
     gene            complement(6259..6861)
                     /locus_tag="EC80_RS23540"
                     /old_locus_tag="EC80_023545"
     CDS             complement(6259..6861)
                     /locus_tag="EC80_RS23540"
                     /old_locus_tag="EC80_023545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BfmA/BtgA family mobilization protein"
                     /protein_id="WP_005812751.1"
                     /translation="MEKDSKTTVAVERTTFTKLDRLAKANNVSKMEFLTHAINYFEKYG
                     INPVEHESPAQEMQKLIKRMDQVFAFLKKQETDLVRPACEALAGASTQITISLSSLLSE
                     EKFRRFLKENKELLAEIINSSDNNGKAIGRTERAVRDSDSTIRKNQDIIFKRIEASETA
                     QIKAYKHIVSFLDAKGKTGLLDDINKVYEREKERRGL"
                     /name="BfmA/BtgA family mobilization protein"
     gene            complement(7351..7620)
                     /locus_tag="EC80_RS23545"
                     /old_locus_tag="EC80_023550"
     CDS             complement(7351..7620)
                     /locus_tag="EC80_RS23545"
                     /old_locus_tag="EC80_023550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671563.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_032536520.1"
                     /translation="MYEIEFSKEAVKHVLLMRKSSPQLFRKLEKLLDELREHPYTGTGH
                     PEQLRYLEGVWSRQLDKKNRIRYMVNETTVIVFIISAWGHYDDK"
                     /name="Txe/YoeB family addiction module toxin"
     gene            complement(7613..7879)
                     /locus_tag="EC80_RS23550"
                     /old_locus_tag="EC80_023555"
     CDS             complement(7613..7879)
                     /locus_tag="EC80_RS23550"
                     /old_locus_tag="EC80_023555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005812757.1"
                     /translation="MQIVTTREFRANQKKYFEMAETETILVSRRNAAPIMVCAVREGDF
                     PSKEELAAIQRGIEDIRNGNTFRMAKNESLDDFLNRIEGEGNV"
                     /name="hypothetical protein"
     ARG             4328..4836
                     /name="nimE"
                     /gene="nimE"
                     /gene_name="5-nitroimidazole reductase NimE"
                     /tool="amrfinderplus"
                     /drug_class="NITROIMIDAZOLE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2..981
                     /name="rep_cluster_1079"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.068"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5367..6262)
                     /name="MOBB"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.993"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaatag taaacaaaga ccttatccaa agttatatta tgactactgc aaaatatgac
       61 tttaacgtgt acgaaaagcg tatcatttat cgtcttgtag aaatggctca aggagagctg
      121 caaggtataa agttcgctga taactgtcag aagatagagc atgatctttt cagttgcgta
      181 aatataacca tgcctattgc ctgcctgctt aatggagagg acgacaaaaa ttatgcaaga
      241 gtaaaagaag ccctacagtc gcttcagcga aagatctttg agtatgaaga tgatgaaatc
      301 tggcaaagta tatctatcat agccttgcct tatatacgaa aacgttcttc agtcttgtcc
      361 tttcaggtgc acccacgtgt atgggactgc atacttgatt tctccaaagg cttcagaaaa
      421 tacgaattga aagctgccat gagctttaag agccagtttt caatgcgctt ttatgagttg
      481 ctgagcaacc agaaaactcc tctgatatac tctatagaac agctaaagga aatgttttgt
      541 gtggcagata agtatgagcg aataaatgat ttcatgcgca aagtagtaga agctgcaaaa
      601 aaagagcttg atgaagtcag cccttatacc tttgagtaca caccgctcaa aacaggacga
      661 aagataacgg ccataaagtt ttatccggta tatcaaccag aacatcgtga tagcgatctg
      721 gaaaaacacg accttcaaaa acagacagct ttaagctggt ctttggctcc tgaagtaaga
      781 agctatctta aaaactcgat tgaattttca gataaagaga ttaagaacaa cctggatttg
      841 tttatatcag cacaaacctt actcccggat ttacttaacg agcttgccat cctaaaaggg
      901 aaatcaaggg aaaagaagaa ccccaaaggt tatataatca atgccatcaa gggaaaaatt
      961 aaagatagaa agaaactttg aatctgtaac aaaatcaaaa agcaacgata tgaaagaagg
     1021 agatttgatt ttggtatctg ctgaagccac aggcttaggc aagccaatgg aagccgttat
     1081 agacaagatt gaaacattca tgggacaaac ccttgtaacg gtcacttaca cgcaacctga
     1141 tgccttatcc ggcttcggag ggtgctttgt ggattctcat atcactttaa gcgaagaaaa
     1201 ggccaagtaa cctgaatata tttcaggaat attcagtata tttacagcat attcaattaa
     1261 aaagaagtat aaaatcagca tatcaagcat attataagca tatttatatc aatcggttaa
     1321 ggtatgacag ataaagtaac catacaaccc aaaagtaagc cggaaaagac tacaatctca
     1381 ataagcggag aaacatctga acggctggag atatactgcc gggcaaacgg catattacgg
     1441 aaagagttcg tttctctcgc cctggaatac tttgaacgca caggtttcga cctgaagagt
     1501 aacgcacttg attactcccc cttggaaaag atcatcggag aattgaatga agtaaaagcg
     1561 accatgcaga caagcaatga aggaacagaa gcgattcgcc aactactaca ggcagtccgg
     1621 gaacagaccg caaaacagct tcctgctcca gaacttatcg caaaggcagc agaagaaaag
     1681 gtacgtgctg aaatgaagtc tgaagaacag gttaaagagc tccaggttct tcaggaagaa
     1741 aacgaccgat tgcgtaacga aatcaaagcc cttcaggagt ataaggaaaa ggctcaccga
     1801 gaactttgcc gggtacgtga tgaacagaaa actttcggga aaataaaagt aaatacagag
     1861 ttataaaata tcaattatga aaaagaacga atttagaaag gaattggtac tgattttagt
     1921 actattatgc acaactatcg catatgcaca aaagaatgat tcgtcattta tggttgctcc
     1981 tgctgatagc acaaaggaca taagcctaac tcaaagagtt gattctcttg agcatgaatt
     2041 atcatatata aaattatcgt atgaattata tacacttaac aacgatttgt ctatatttaa
     2101 taacgatata ggtgtcagaa ctaatgcaat acaaataaat atctatacac acaattttga
     2161 ttataaatta tacaaagcat acaaagacaa ttacgaagca accaaagaca aactactatc
     2221 atatcaagat ttaattgaaa caaagaaaac ttatttctgg cttaaaatta ttacatatcc
     2281 ttatactgag gttgagaaga aactgctatt gtcaagttat atgacaattg ataaaggata
     2341 ttcccaatta gagtcttcat taaagctcat gaaaatatgt cttgatatgt acaaagattc
     2401 tttatagagt tattcttatt gacacaaaga accatactct cagctataaa attacatacc
     2461 cattccaaaa ccccggcttc tttccctggg ctggtcgagt tcttcctcct gttgcttctt
     2521 ccgctgcggt gcctgattga tttccggaac ttgggatata ctgtttccct gttgttggct
     2581 ctcttcacgg ccttgttcct ggtgcggtgg ctccggaact tatagtccaa gcaacagaag
     2641 caaaggcgaa ggcagaaagt ctggctacag aacgtgccaa agaccttcag ctccttcagg
     2701 aagagaacaa ccgattacgt aacgaaatca aaattcttca ggaatataag gaaaaagcac
     2761 accgggaact ttgccgggtg cgtgacgaac agaaaacgat agggaaaata aaagtaaata
     2821 cagaactgta acagccagtc gggtagaaaa taggtagaaa aacggcatgg taaacgattg
     2881 gaactttggc tcttttttat aactttgtgg ctaactatgg agtacattcc cataggacat
     2941 atgttaataa ataaataaat tctattaggg caagtctttt tcctctttcc ctcagtcggt
     3001 gaggactgat aacccctttt agcttttatt ttattgttgt attagtattt cagtctgtga
     3061 agatatgagg aaatacatgg gggcaagagt gccccttttt tttggcaaat aaaaatggcg
     3121 accgaaacca gccgccatgt cccatgcatt tccgcaatgg gcttgccaga gcctacgctt
     3181 gagttctaca aatataagca attccgtttt cttctgcaat tataacggca caataccggg
     3241 acagttttga gcaactggct cctgtaactt tttccggata tctgcaaatt tgttttgtga
     3301 acggcaaaaa ttctttgtga acagcactaa gtccatactc tcagaataaa gaagtgattt
     3361 aaactttttg ttttcgtgaa attttcttca ataacaagac tgcaaatgcc aagtaattcc
     3421 aagacatcca acttgaaaca gttgtatcga accggttcag tagcgtcctg aaactgtcca
     3481 tccaagcgtt ggttcgttcg atggaatacc tttcagcata tagttcatca tcaacaaaga
     3541 tgtcatcggc attttctctc cgtttattga tgcagatgtt tgcgatgatc tctttttcac
     3601 ccaggaaacc acgtaagaca tcacaatcga atccggcatc ggcattgagg aaaagaccat
     3661 caacccggat atgggatttc tcaaggtcgt tgaacatgtc accgatgacc ttttctatgt
     3721 catgtacgtc atggtgttcg ccagatttcg gagaagacat ggcaatcgga agtccttgcc
     3781 tgtctgtcag gtagagtgcg ttggtcgtct tccgtttctt ccgtccctgg tatccgactt
     3841 cttcaccgcc acggagtgca gccgtgtggc tcccgtccaa atccacgctg gacatgtcca
     3901 acttatggcg gtgtttgtca agcaacctga cccacatgga tttccactcg cccgacttgc
     3961 tccatttgtt gtaatgatga tacacggcac catagctcag taccttgccg ctaaacaggg
     4021 cttccacagg aaggaacgcc cattgacaac cggtcttcaa cttgtacaag atggcgttta
     4081 ccacctctat gagacaactt tgagtggcat aaccacgttt tgccttggaa agatgaggca
     4141 atatttcatt ttttatcgta tctttgtcga gcatagtgta cattggactt tcactatata
     4201 tgagtttggt caccataata tagggaacaa ttcaatttgt acgctgtgct ttaacatttt
     4261 gatacgattt taagagaatg aaataagttt aaatcacttc attgtttaat actaaagatc
     4321 agtaatatgt tcagagaaat gcgtcgtaaa cgacagttgt tgccacaaga agagagcgtg
     4381 gcaattcttg aaaaaatgac aaacgggact ttagctctcc acggagataa tggctatcct
     4441 tatgccgttc ctctcagtta tttttatgct gatggcaaaa tctattttca ttgcgccaag
     4501 atagggcata aggtggacgc tattatgcaa aacaataaag tatcgttttg cgttgtggaa
     4561 caagacaata ttaaacctgc cgaattcacg acctactttc gaagtgtgat agtctttgga
     4621 aaggcctata ttttaaccga cgaaacagaa aaacgtatgg ctatgacttt gctggtgaat
     4681 aaatattctt tcggtgagcc aggtttgtct gatgaaatag ccaaaagcat caaccatcta
     4741 ctgatggtaa agatcgatat cgaacacatg acgggcaagg aggccatcga tttggttaga
     4801 gagaaagaaa agttttgtac ttcagagaaa acctcttgaa aaaacgattt agatgagtac
     4861 gtcgagagat ttaaggaaaa gagtattttt ctgtactcac taaccctgcg ggtaaccgca
     4921 taagacttta taccgactaa tatgtaccga taaaaatatg gctgcaagtt atttcatttg
     4981 cagctatatt tttgctcgta atcaataaga aacgacaaaa agcattacct ttgtaaaatg
     5041 attcaagagg gcttcaaatt ttacaagtct tgcaaggagt tacaacctta cgtcaggtat
     5101 tattgggtat tcaaaagcaa tcaaccgctg aataccctta cttttccgat tggctgtcct
     5161 caaatcattt tccacaaaca aacaccgctt tacatcccag aattgaaaac tgctcaatca
     5221 gaatttacta ttagtggtca ggtaaattat ccttcccatt tatatgcgaa tggtaatgta
     5281 gaaatgattg tagttgtatt ccagccatat accctaaaag cattcttgaa cttgcccata
     5341 tctttattgc acaatcagaa aatcacatac ccattccaaa accccggctt cttttcctgg
     5401 gctggtcgat ttcttcctcc tgttgtttct ttctctgcgg tgcctggttg atatccggaa
     5461 cttgggatat actgtttccc tgttgttggc tctcttcacg gccttgttcc tggtgcagtt
     5521 cctgaagatc ccggctccat tcggccttca tcttctcaag ccgttcatgg ttcacacgtt
     5581 ccgcccattc ctttttctgg aagatttctt tcgggcttcc gttcttcatg gtattgagat
     5641 agtcgaatgt ctgttccgga gcccggtcat accctgtgcg cttatcaaat gaggtttcac
     5701 actttcggaa aaaatctgtt ctgtcaaaac cgcccttcac gtttccgcag ttcttcttcc
     5761 cggtgtggtt ggtctttggg cttagcttcc tggtgttgct cctgtccttc cggctgacaa
     5821 tgatatgtgc gtgcatgtcg taccggtcgg ctcccttcct ctcgaaatgg atttttccgt
     5881 aatactcgat atcctctttg ttcagacctt ttccgaaccc ttccgcatag tgctgcatca
     5941 catcatcacg gacatacttt ctcatggctt cagcctgttc cttctctgtc ttgcccattt
     6001 tctccagctc ccggctggaa gggcttaccg tgatcacata gaacttcgca tcgttccggc
     6061 ttaattttgc cttgttgttg tctattttga aggttacttc ctgtgcgctt atgtagtcac
     6121 gtgagttatt gaaaaacggc tgcacttcct ggccgttctt catgcgctcc agatcctcat
     6181 gctggaggta ggtcgttact cctgtgcagc tccctgtatt ggcataggtt ccggaaccgc
     6241 caccctgaat ctttatgttc atagtcccct cctttccttt tccctctcat agaccttgtt
     6301 tatatcatcg agcagcccgg tctttccttt tgcatcgagg aaggaaacaa tatgcttata
     6361 ggctttaatc tgtgccgttt cgctggcttc tatccgcttg aagataatat cctggttctt
     6421 cctgatagtt gaatcggaat cccgcaccgc cctttctgtc cggcctatgg ccttgccgtt
     6481 gttgtcggaa ctgttgataa tctcggcaag cagttccttg ttctccttca ggaaccgcct
     6541 gaacttttct tccgaaagta aggatgaaag gcttatggtt atctgtgtgc tggctccggc
     6601 cagagcttca caagccggac gcacaagatc cgtttcctgt ttcttcagga atgcgaacac
     6661 ctgatccatg cgcttgataa gtttctgcat ttcctgtgct gggctttcat gctcgaccgg
     6721 gtttataccg tacttctcaa agtagtttat agcatgtgta aggaactcca tttttgacac
     6781 attgtttgcc ttggccagac ggtcaagttt agtaaaagta gtacgttcta ctgctaccgt
     6841 tgttttgctg tctttttcca tgatattata aggcttatta taatgaatta atatactaac
     6901 ttattggttt acaagatagt atatttattc attataacca aattatatta gtataatttt
     6961 ctaaataact gatttacatc taaaaactcc gtagggcaag aggaaaaaac agggcttgcc
     7021 cggtttcctc ttgctgttct atcgaacacc ctccccagcg cgaaattagc aaaggggacg
     7081 gggagagata gaacctatcc cacacacgta aacacacacg aaaaccgcac gcccggtaca
     7141 caacagaaat acccaacaaa accgcacaac agaacgtgtc cggaacacca caaaaactca
     7201 tcggtcgggg agcgccgcac ggcaaggggg tgtaccaaca ctcccgacga gtgcagccgt
     7261 ttgcttggat atatgatata agttattttg attctgtcac aaattcaaaa ggattgcagt
     7321 atgtgccgtt tcggaactta ctgcaatcct tcatttgtcg tcataatgcc cccaggctga
     7381 aatgataaac acaattacgg tagtctcgtt gaccatatac ctgatacggt tcttcttgtc
     7441 cagctgcctt gaccagacac cctccaggta tctcaactgt tccggatgcc ctgtacccgt
     7501 gtacgggtgt tcccggagtt catcaagcag cttctccagc ttcctgaaaa gctgcggaga
     7561 ggattttctc atcaacagga catgttttac cgcttctttg gaaaattcta tctcatacat
     7621 tgccttcgcc ctctatacgg ttaagaaaat catccagaga ctcgttttta gccattctga
     7681 aggtatttcc gtttcggata tcttcaatac ctctctggat ggccgcaagt tcttcctttg
     7741 aagggaaatc accttctctg actgcacaca ccataatagg ggctgcattg cgtctggaga
     7801 ccaggatcgt ttccgtctct gccatttcga agtatttctt ctggttggca cgaaattcgc
     7861 gtgttgtaac aatctgcata gttctaatat attggtttac actgcaaaaa tagggataaa
     7921 aaccaataaa acaaaagttt tgtgtactta tttgtgtaca cgcgcggaaa gttcgttcct
     7981 caaagaacgc tgcgctggcc tttgctttgc tttcacaaac attcaaaaag tcgtttttca
     8041 cggatttgaa ggtctggaag ccacattccg gctaaaattt tatgacttgt ttttaagcat
     8101 tacttgttat atgcttatcg acttccgaaa aacatgcttt aacaatctga taaacagtaa
     8161 attacaagca acaacaagaa tccaactacg actttttgaa tactaactac gacttttcga
     8221 atactatttc aaagtttaac tacgactttt tgaatactaa tcacacaatc tacgactttt
     8281 atttgttaaa gtcgttttta atacttatat ttgcacaaaa cactgataat t
//
