LOCUS       NZ_CP036545             2782 bp    DNA     circular CON 25-JUL-2023
DEFINITION  Bacteroides fragilis strain DCMOUH0018B plasmid pBFO18_3, complete
            sequence.
ACCESSION   NZ_CP036545
VERSION     NZ_CP036545.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02730071
            Assembly: GCF_000724665.2
KEYWORDS    RefSeq.
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Sydenham,T.V., Hasman,H., Wang,M., Soki,J., Nagy,E. and
            Justesen,U.S.
  TITLE     Identification of resistance genes in three multidrug-resistant
            Bacteroides fragilis isolates by whole genome sequencing
  JOURNAL   (in) ? (Ed.); THE 24TH EUROPEAN CONGRESS OF CLINICAL MICROBIOLOGY
            AND INFECTIOUS DISEASES; ECCMID 2014, Barcelona, Spain (2014)
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Sydenham,T.V., Hasman,H. and Justesen,U.S.
  TITLE     Complete genome assembly of MDR B. fragilis
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 2782)
  AUTHORS   Sydenham,T.V., Hasman,H. and Justesen,U.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2019) Department of Clinical Microbiology, Odense
            University Hospital, J.B. Winsloews Vej 21,2, Odense C 5000, Denmark
REFERENCE   4  (bases 1 to 2782)
  AUTHORS   Sydenham,T.V., Hasman,H. and Justesen,U.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2014) Department of Clinical Microbiology, Odense
            University Hospital, J.B. Winsloews Vej 21,2, Odense C 5000, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: OUH_BF18_3.manual
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/25/2023 03:56:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,424
            Genes (coding)                    :: 4,355
            CDSs (with protein)               :: 4,355
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 13 of 69
            Pseudo Genes (incomplete)         :: 41 of 69
            Pseudo Genes (internal stop)      :: 23 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036545.1.
            Bacteria and source DNA available from Ulrik S. Justesen.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="DCMOUH0018B"
                     /isolation_source="Blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="pBFO18_3"
                     /geo_loc_name="Denmark: Odense"
                     /lat_lon="55.3958 N 10.3886 E"
                     /collection_date="2011-10-10"
                     /collected_by="Ulrik Stenz Justesen"
     gene            join(1803..2782,1..124)
                     /locus_tag="EE52_RS23450"
                     /old_locus_tag="EE52_023455"
     CDS             join(1803..2782,1..124)
                     /locus_tag="EE52_RS23450"
                     /old_locus_tag="EE52_023455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_005783389.1"
                     /translation="MGATSIHVQAVKPGSEIHNFREKELDYVRPELSHLNESWVGDSIS
                     HRLESAKQRYFDTVGQKMQTKAAPIREGVIVIKQETTMQELQQFAAVCKERFGIEAFQI
                     HIHKDEGYMNAKQWTPNLHAHVVFDWTQPNGKSVRLSRDDMAELQTIASEALGMERGVS
                     SDRKHLSAMQYKTECAKEQLQELSNDISSALDKHKDVQNQLLQLQKELRSIETKKNVQK
                     LISKASEKFYGLIGKTVNDREKDTLKAKIKALEGENEQLSDRLGKAILEKERNGTKVFK
                     AENDKEYYRQQMDNARTTSNRLRTENQELKTETKELKKELGKMKDLFNSEQLEALRHHF
                     PNISKAMEEGKDLLKQITRSRGFGMGM"
                     /name="plasmid recombination protein"
     gene            595..1185
                     /locus_tag="EE52_RS23455"
                     /old_locus_tag="EE52_023460"
     CDS             595..1185
                     /locus_tag="EE52_RS23455"
                     /old_locus_tag="EE52_023460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA protein"
                     /protein_id="WP_175414010.1"
                     /translation="MENKKVLKLTDFQKNEENPFMKQAIEGIENHVVKKYKSSTGSDKK
                     AVVAVADTDTGEVFKTSFIRQIEVDEEQFAKLYLANFAAFFNLSQSAIRVFGYILTCMK
                     PKNDMIIFDRRKCLEYTQYKSDKAIYKGLAELVQSEIIARGPNEYNWFINPLIIFNGDR
                     VSFTKTYVRKKTLAAQKKEEAEKRQLSLGFDEL"
                     /name="RepA protein"
     Typing          596..1175
                     /name="rep_cluster_1868"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.308"
                     /coverage="95.0"
                     /evalue="1.26e-119"
     Typing          1804..2726
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.779"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 gttcaattcc gaacaactgg aggctttaag gcatcatttc ccaaacatat caaaagctat
       61 ggaagagggg aaagacctac tcaagcaaat caccagaagc agaggtttcg gtatgggtat
      121 gtaacccccc cgcccccaaa gggggagcga ccaaacggca gcttcactca atggagtgtt
      181 acgccgttcg ggttcggaag aaattttgca gggttcgaaa aacggttgtg tgcttattcg
      241 aaaaaaagtt tatggtgtgg gcgctcgtcg ggacgggtgg tcccgcccct tgccgctggg
      301 cgctccccga ccgatgattt tttaagtagt tgattatatg ccgtttttag ggtataataa
      361 gaacttaaga aatagaataa gttaagtagt tgatactcaa tatagggtat tttccatatt
      421 ggaaaatcag attttccata ttagaaagtc actctttcct attagatgtt aaaaaggaaa
      481 atatattttg cttttttatt gtattaatgg aaatttaaat attcctttgt ggaaaatcaa
      541 attatacttt ttcaccgata tatagaaagt caaatttgcc taaatagaat gtttatggaa
      601 aataagaaag tactcaaatt aaccgatttt caaaaaaacg aagaaaaccc ctttatgaaa
      661 caagcaattg aagggattga aaatcacgtt gttaagaaat acaaaagtag tacgggaagt
      721 gataaaaaag ctgtagttgc tgttgctgat acagatacag gagaagtatt caaaacctcg
      781 tttatccgac aaatagaagt agatgaagag caatttgcaa aactatacct tgcgaatttt
      841 gccgcttttt tcaatctatc acaatcagct attcgggttt tcgggtatat tctaacttgc
      901 atgaagccta agaatgatat gataattttc gataggcgaa aatgcttaga atacacccaa
      961 tacaaatcag acaaagccat atacaaagga ttggcagagc tcgtacaaag tgaaatcata
     1021 gcaagaggtc caaacgaata taattggttc attaatccgt tgattatctt taatggggat
     1081 agggtttcat ttacaaaaac atacgttcgg aaaaagactt tagctgccca aaagaaagaa
     1141 gaagcagaga aacgacagtt atcacttggt tttgatgaac tgtaacactc cattgagtga
     1201 agctgccgtt tggtcgctcc ccctttgggg gcgggggggg atagataaag ttcctctatg
     1261 taaagttata atgggggatg aaaggcaagg ttcgctaacc ttaccccgat tgtttatgct
     1321 tcccagcatt caatctatcc ccgtttatgg gtttctcttt caccctacaa agataacctc
     1381 atgggggaaa aatgtccaag aatatggggt aaaaactatc aagtcggtag aaaataagta
     1441 tctttgaagt acatttttag tagaggtact ggtatgccta gacgaagcag ataggcaaaa
     1501 ataaagccgc tggggatgtc ggatgtccgt caattaactc cagcggcttg tattttggta
     1561 actctcccgt atcggtcagc taccgtttgg tcagcaacaa aggtagtact ttattgtcgc
     1621 aaatccaaat gttttcccct ttatttgtgc atttacaaaa cattcactaa cttcgcacct
     1681 atgcagtatg gtgatagcac actagcaccc taaagggtac gtgtgtaagg ggacaagccc
     1741 ccttaacccc ctgtcagccg gctgtcaccg gctgttatcg attgtcacaa aaatgttaac
     1801 ttatgggagc aacaagtatt catgtacaag cagtgaagcc ggggagcgag attcacaact
     1861 ttagggaaaa agagttggac tatgttcgtc cggaacttag tcatttgaat gaaagctggg
     1921 ttggagatag catctcccat cggctggaga gtgcgaaaca gagatatttc gatacggttg
     1981 ggcagaagat gcagactaaa gcagcaccca tacgggaggg ggtaatagta atcaagcaag
     2041 aaaccaccat gcaggaactc cagcaattcg ctgcggtctg caaggaacgt ttcggtattg
     2101 aagcgtttca aatccatata cacaaggacg aaggatacat gaacgcaaag caatggacac
     2161 ctaaccttca cgcccatgta gttttcgatt ggacgcagcc gaacgggaag agtgtgcgtt
     2221 tatcgcgtga tgacatggca gaactccaaa ccatagcatc tgaagcactg ggcatggaac
     2281 gtggtgtttc ttctgatcgc aaacacttat cggctatgca gtacaagacc gaatgtgcga
     2341 aagaacagct tcaggaacta tccaacgata tatccagtgc gttagacaag cacaaggacg
     2401 tgcaaaacca gcttcttcag cttcagaaag aactacgttc cattgaaaca aagaaaaacg
     2461 tccaaaaact catttctaag gcttcagaga agttttacgg cttgataggg aaaacggtta
     2521 acgataggga aaaagatacc ttaaaagcca aaataaaggc tttagagggg gaaaatgaac
     2581 agctatccga tagactggga aaggctatac ttgaaaaaga gcgaaacggc acaaaggtat
     2641 tcaaagccga gaacgacaag gagtattata gacagcaaat ggacaatgca aggacaacca
     2701 gtaaccgact aagaacggag aaccaagaac taaagaccga aaccaaggaa ttgaaaaaag
     2761 aacttggtaa gatgaaagac tt
//
