LOCUS       NZ_CP036544             4137 bp    DNA     circular CON 25-JUL-2023
DEFINITION  Bacteroides fragilis strain DCMOUH0018B plasmid pBFO18_2, complete
            sequence.
ACCESSION   NZ_CP036544
VERSION     NZ_CP036544.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02730071
            Assembly: GCF_000724665.2
KEYWORDS    RefSeq.
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Sydenham,T.V., Hasman,H., Wang,M., Soki,J., Nagy,E. and
            Justesen,U.S.
  TITLE     Identification of resistance genes in three multidrug-resistant
            Bacteroides fragilis isolates by whole genome sequencing
  JOURNAL   (in) ? (Ed.); THE 24TH EUROPEAN CONGRESS OF CLINICAL MICROBIOLOGY
            AND INFECTIOUS DISEASES; ECCMID 2014, Barcelona, Spain (2014)
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Sydenham,T.V., Hasman,H. and Justesen,U.S.
  TITLE     Complete genome assembly of MDR B. fragilis
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4137)
  AUTHORS   Sydenham,T.V., Hasman,H. and Justesen,U.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2019) Department of Clinical Microbiology, Odense
            University Hospital, J.B. Winsloews Vej 21,2, Odense C 5000, Denmark
REFERENCE   4  (bases 1 to 4137)
  AUTHORS   Sydenham,T.V., Hasman,H. and Justesen,U.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2014) Department of Clinical Microbiology, Odense
            University Hospital, J.B. Winsloews Vej 21,2, Odense C 5000, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: OUH_BF18_3.manual
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/25/2023 03:56:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,424
            Genes (coding)                    :: 4,355
            CDSs (with protein)               :: 4,355
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 13 of 69
            Pseudo Genes (incomplete)         :: 41 of 69
            Pseudo Genes (internal stop)      :: 23 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036544.1.
            Bacteria and source DNA available from Ulrik S. Justesen.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="DCMOUH0018B"
                     /isolation_source="Blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="pBFO18_2"
                     /geo_loc_name="Denmark: Odense"
                     /lat_lon="55.3958 N 10.3886 E"
                     /collection_date="2011-10-10"
                     /collected_by="Ulrik Stenz Justesen"
     gene            106..1131
                     /locus_tag="EE52_RS23410"
                     /old_locus_tag="EE52_023415"
     CDS             106..1131
                     /locus_tag="EE52_RS23410"
                     /old_locus_tag="EE52_023415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657007.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_008657007.1"
                     /translation="MKKKLPITKNKDVVVSWVYTWSKQQDMSIHEQRIVLRILEACQAE
                     LKGVKLKDYAGTKRKFEHGLWDVDAQMHVSDVIFSGRDYNEIIAALDSLAGRFFTYEDD
                     EEWWKCGFISNPKYKKHTGIITFRVSNDLWGVFTKFAKGYREFELNKALALPTGYSLRF
                     YMLMSGQVYPLDISLDNLKERLGIPADKYKDKNGKDRIDNFEERVLKPAKAALDESCPY
                     TFNYVKVRENPNNKRSKVTGFRFYPVYQPQFRDEELEGKDLQAKVTARYQIDSHVYEYL
                     RYSCGFTSEEINRNKETFITAQEKITDLIGELALLNGKSREKNNPKGWIINALKGKIKD
                     K"
                     /name="replication initiation protein"
     gene            complement(1168..1398)
                     /locus_tag="EE52_RS23415"
                     /old_locus_tag="EE52_023420"
     CDS             complement(1168..1398)
                     /locus_tag="EE52_RS23415"
                     /old_locus_tag="EE52_023420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295510.1"
                     /translation="MGTTEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLALAREKLSSEEIQQQMKELGVEMEIVPLI"
                     /name="hypothetical protein"
     gene            complement(1418..1870)
                     /gene="mbpC"
                     /locus_tag="EE52_RS23420"
                     /old_locus_tag="EE52_023425"
     CDS             complement(1418..1870)
                     /gene="mbpC"
                     /locus_tag="EE52_RS23420"
                     /old_locus_tag="EE52_023425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpC"
                     /protein_id="WP_004295511.1"
                     /translation="METNSHPEAAASNVKQGQYSPRKRKDTTPRVNPQLAVELVYQELK
                     RVEVYTKRIEDATARKVQIDGKSLESAENRLKNVLADFERQGYRMKNGGYVDKRISFYS
                     ILCAVISLLFACFMCYLWTDAAKDRDNYKQYYEYYQEQAREQKGNK"
                     /name="mbpC"
     gene            complement(1827..2621)
                     /gene="mbpB"
                     /locus_tag="EE52_RS23425"
                     /old_locus_tag="EE52_023430"
     CDS             complement(1827..2621)
                     /gene="mbpB"
                     /locus_tag="EE52_RS23425"
                     /old_locus_tag="EE52_023430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpB"
                     /protein_id="WP_007560590.1"
                     /translation="MIGKGKSISHGVAALEYDLAKEINGQAVATEIARHELYGCTGAEM
                     VQEMKPYHIDFPNVKNNCLRFEVSPSIEESATFTDADWAELGNDFMQRMGLANHQYIII
                     RHSGTESKKEQAHLHILANRVSLSGELYRDNWIGKKATEAANAIAKERNFVQSQDIGKV
                     NKAEIKEAMDGVLKKMQGFDFTKFKEELGKRGFKVREARASTGKLNGYYVTARSGTEYK
                     ASEIGKGYTLAHIERTQSKLKCNSMNISHGNKLTPGSGSFQR"
                     /name="mbpB"
     gene            complement(2618..2917)
                     /gene="mbpA"
                     /locus_tag="EE52_RS24175"
                     /old_locus_tag="EE52_023435"
     CDS             complement(2618..2917)
                     /gene="mbpA"
                     /locus_tag="EE52_RS24175"
                     /old_locus_tag="EE52_023435"
                     /inference="COORDINATES: protein motif:HMM:NF041418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpA"
                     /protein_id="WP_004327437.1"
                     /translation="MELRRNEKITFRCTELEKDALAEQAARCSLSVSEYCRSLSLGGRP
                     RERYTEEERQLLRDIAQLKGTLQRLNNYFGGRQYREVFEENRALITELKKILSR"
                     /name="mbpA"
     gene            complement(3099..3296)
                     /locus_tag="EE52_RS23435"
                     /old_locus_tag="EE52_023440"
     CDS             complement(3099..3296)
                     /locus_tag="EE52_RS23435"
                     /old_locus_tag="EE52_023440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_007209655.1"
                     /translation="MVEYCVYWLENGEPMHEVFSNLAAAEMYSCAIRGKENVEWVEVSE
                     EEAIDLDELKDMFPDDFCGV"
                     /name="hypothetical protein"
     gene            complement(3307..3582)
                     /locus_tag="EE52_RS23440"
                     /old_locus_tag="EE52_023445"
     CDS             complement(3307..3582)
                     /locus_tag="EE52_RS23440"
                     /old_locus_tag="EE52_023445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /GO_process="GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="WP_004327436.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            complement(3579..3830)
                     /locus_tag="EE52_RS23445"
                     /old_locus_tag="EE52_023450"
     CDS             complement(3579..3830)
                     /locus_tag="EE52_RS23445"
                     /old_locus_tag="EE52_023450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295507.1"
                     /translation="MEALSVREYRNNLAASFTKADNGEQVLIRRKNEIYALVKVGREDL
                     MITPELQARIDKAREEIKSGKCVTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
ORIGIN
        1 ctttgggagt ttttaagacg cactttccct acactttggg agttttaccc ttcttttttt
       61 gtttatatgt aggtatttca tatctttgca acaaatcaaa tagttatgaa aaagaagcta
      121 cctattacca aaaataaaga cgtagttgta tcatgggtat atacatggtc caaacagcaa
      181 gacatgtcca tacacgaaca aagaatagtt cttcgcatac tggaagcgtg ccaggctgaa
      241 ctaaagggag taaagctgaa agattatgca ggcacgaaac gcaaatttga gcatggactt
      301 tgggatgttg atgcacaaat gcatgtatct gacgttattt tttccggacg tgactacaac
      361 gaaatcatag ctgcactcga ttcactggct ggacgttttt tcacttacga agatgatgag
      421 gaatggtgga aatgtggatt tatatctaac ccgaaataca aaaagcatac cggtattata
      481 acatttcgtg tatctaatga cctttggggc gtgtttacca aattcgcaaa aggatatagg
      541 gagtttgaac taaacaaggc tcttgcgctg cctacgggct attcgcttag gttttatatg
      601 ctcatgagtg ggcaggtgta ccctttggat atatcactgg acaacttaaa agaacgactt
      661 ggcatacctg cggacaaata caaagacaaa aacggaaaag acagaatcga caacttcgag
      721 gaaagagtat taaaaccagc aaaggctgcg cttgatgaga gttgcccata cactttcaac
      781 tacgtgaaag tgcgtgaaaa cccaaacaac aaacgtagca aggtgaccgg gtttaggttt
      841 tacccggtct atcagccaca gttcagggat gaagaactgg aagggaaaga tttgcaggca
      901 aaggttacag cacgatatca gatagacagc catgtatacg agtatctccg ttattcctgc
      961 ggtttcacat cggaagagat aaaccggaac aaagagacat tcataacggc acaagaaaag
     1021 ataaccgacc ttatcggaga actggctctc ctaaacggaa aatcacgaga aaagaacaat
     1081 ccgaaagggt ggattataaa cgcccttaaa gggaaaatca aggataagta acaagattac
     1141 ccggctgaaa cactccgggt aatcttgtca aatcagagga acgatttcca tttctacacc
     1201 aagttctttc atctgctgtt gtatttcctc gctgcttagt ttctctctcg ccagtgcaag
     1261 cccatgaaag gcgtcccagc ctgccggctt cgaaacgata ctaccgttta ggacggcatg
     1321 ggtgatttcg ccgccggact tcgcaccctt gttcagtatg aagaagaacc ggggttcttc
     1381 gtgttccgtt gttcccataa ttcgtaaagt tagatgttta tttattcccc ttttgttccc
     1441 ttgcctgctc ttggtagtat tcataatact gcttgtagtt gtcccggtct ttggctgcat
     1501 ccgtccaaag atagcacatg aaacaagcga acaataggga gataacagca caaaggattg
     1561 agtagaacga aattcgtttg tccacataac cgccgttttt cattctgtac ccctgccgtt
     1621 caaagtccgc caacacgttt tttaggcgat tttcggcact ttcaaggctt ttcccgtcta
     1681 tttgtacctt tcgggctgtt gcgtcctcta tgcgcttcgt atagacttca acacgcttta
     1741 gttcttgata caccagttct accgcaagct gcgggtttac ccttggcgtt gtatcttttc
     1801 ttttccgggg gctgtactgt ccctgcttaa cgttggaagc tgccgcttcc gggtgtgagt
     1861 ttgtttccat gagatatgtt cattgagttg cacttcagtt tgctttgtgt ccgctcgata
     1921 tgggcgagag tgtagccttt ccctatctcg cttgccttat actccgtacc gcttcgggct
     1981 gtgacatagt agccgttaag tttcccggtg cttgccctgg cttcccggac tttaaaacct
     2041 ctcttaccaa gttcttcctt gaatttggta aagtcaaagc cctgcatctt cttcaatacg
     2101 ccgtccatgg cttccttgat ttccgctttg ttgactttgc caatgtcctg cgactgtaca
     2161 aagttccgtt ccttggctat ggcattggcg gcttccgttg cctttttccc tatccagttg
     2221 tcccggtaca gttccccgga aagggaaacc cggtttgcca gtatgtgcag gtgggcttgc
     2281 tctttcttgc tctccgtacc gctgtgccgg atgatgatgt actggtggtt cgccaacccc
     2341 atgcgctgca tgaagtcgtt accaagttcc gcccagtccg catccgtaaa ggtggcgctt
     2401 tcctctatcg aggggctgac ctcgaaacgt aggcagttgt tttttacgtt cggaaaatca
     2461 atgtgatacg gtttcatttc ctgcaccatt tccgcaccag tacacccata aagttcatgc
     2521 cgggctatct cggtggctac tgcctgcccg tttatctctt tggcgaggtc gtactccaat
     2581 gccgccacac catgtgatat gcttttccct ttccctatca tcgtgagagt atttttttga
     2641 gctctgtaat tagtgcccgg ttctcctcga acacttcccg gtactggcga ccaccgaagt
     2701 agttgttcag ccgttggagc gttcctttca actgggctat gtcccggaga agctgccgtt
     2761 cctcctcggt gtacctctcc cttgggcgcc ccccaaggga caaactccgg cagtattccg
     2821 atacgcttag actgcaccgg gctgcctgct ccgcaagtgc gtccttttca agttcggtac
     2881 atcggaatgt aatcttttcg ttccgtctta gttccatgcc acaaaggtaa tcttaaaatc
     2941 tttttgagca aagcgagaaa agcaagaatt gtccgacaag gacacttctt gctatatata
     3001 cacatgccgc atgggaatac atccacacca accgcccggc aaaaagccca ggcatgttag
     3061 aaaatccccc acagccgaac aatttctacc caaggggatt acacgccgca gaagtcatcc
     3121 gggaacatgt ctttcagttc gtccaaatca atggcttctt cttcggacac ctccacccat
     3181 tcaacgtttt ctttccctct tatcgcacat gagtacatct cggcggcggc aaggttagag
     3241 aacacctcgt gcataggctc gccgttctct aaccagtaaa cacaatattc gaccataaca
     3301 gttttatcat ttatcgttgt aatgcccctc tacctccaaa atatagactt ctactacttc
     3361 ctcgtaaatg tcatagatta tccggtcatg ggcggtgatg tgtcgagacc atgtaatatc
     3421 cccaccgcct cttaacgctt cgggatgccc aagcccagtc ttagggtgct ctaacaattc
     3481 tttgtagatt ttcttatact tcttgaacaa attggggttt gatttcttcc atttggcaat
     3541 caccttatcc accccgtcgg aaaccctaat tgtgtacatc atagactatc gaaataagcg
     3601 tcaatatcct cactgctttt gagggtaaca cactttccgg attttatttc ttcccttgcc
     3661 ttgtcaatcc ttgcttgcag ctccggggtt atcatcaaat cctcacgacc tactttcacc
     3721 aaagcgtaaa tttcgttttt ccgacgaatt aatacctgct cgccattgtc tgctttggtg
     3781 aaagacgctg cgaggttgtt acggtattct cttactgata atgcttccat atctgtttgc
     3841 ttttaaattc agcacaaaga tagctatatt tcagtaaaat acaaatcttt tgtacacatt
     3901 tgtgtacacg ccataaaaac cgttcccaat cctaccgccc gttggttggt tttgctttgc
     3961 tcttttttcc ctatcgtttt tctttttccg acagtaaatc aaccgcttag ccctataaat
     4021 tttccttgtc tgttataaac atcgtctgta atatgctcta tcacttttac gaaaacaccg
     4081 ataaacaaag ggattaaagc atgtgcttcc ctacattttg ggagttttaa tcctaca
//
