LOCUS       NZ_CP036543             7221 bp    DNA     circular CON 25-JUL-2023
DEFINITION  Bacteroides fragilis strain DCMOUH0018B plasmid pBFO18_1, complete
            sequence.
ACCESSION   NZ_CP036543
VERSION     NZ_CP036543.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02730071
            Assembly: GCF_000724665.2
KEYWORDS    RefSeq.
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Sydenham,T.V., Hasman,H., Wang,M., Soki,J., Nagy,E. and
            Justesen,U.S.
  TITLE     Identification of resistance genes in three multidrug-resistant
            Bacteroides fragilis isolates by whole genome sequencing
  JOURNAL   (in) ? (Ed.); THE 24TH EUROPEAN CONGRESS OF CLINICAL MICROBIOLOGY
            AND INFECTIOUS DISEASES; ECCMID 2014, Barcelona, Spain (2014)
REFERENCE   2  (bases 1 to 7221)
  AUTHORS   Sydenham,T.V., Hasman,H. and Justesen,U.S.
  TITLE     Complete genome assembly of MDR B. fragilis
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7221)
  AUTHORS   Sydenham,T.V., Hasman,H. and Justesen,U.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2019) Department of Clinical Microbiology, Odense
            University Hospital, J.B. Winsloews Vej 21,2, Odense C 5000, Denmark
REFERENCE   4  (bases 1 to 7221)
  AUTHORS   Sydenham,T.V., Hasman,H. and Justesen,U.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2014) Department of Clinical Microbiology, Odense
            University Hospital, J.B. Winsloews Vej 21,2, Odense C 5000, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: OUH_BF18_3.manual
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/25/2023 03:56:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,518
            CDSs (total)                      :: 4,424
            Genes (coding)                    :: 4,355
            CDSs (with protein)               :: 4,355
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 13 of 69
            Pseudo Genes (incomplete)         :: 41 of 69
            Pseudo Genes (internal stop)      :: 23 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036543.1.
            Bacteria and source DNA available from Ulrik S. Justesen.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="DCMOUH0018B"
                     /isolation_source="Blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="pBFO18_1"
                     /geo_loc_name="Denmark: Odense"
                     /lat_lon="55.3958 N 10.3886 E"
                     /collection_date="2011-10-10"
                     /collected_by="Ulrik Stenz Justesen"
     gene            1..996
                     /locus_tag="EE52_RS23360"
                     /old_locus_tag="EE52_023365"
     CDS             1..996
                     /locus_tag="EE52_RS23360"
                     /old_locus_tag="EE52_023365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812759.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_044302094.1"
                     /translation="MAIVNKDLIQSYIMTTAKYDFNVYEKRIIYRLVEMAQGELQGIKF
                     ANNCQKIEHDLFSCINITMPIACLLNGEEDKNYARVKEALQSLQRKIFEYEDDEVWQSI
                     SIIALPYIRKRSSVISFQVHPRVWDCILNFSKGYRKYELKAAMSFKSQFSMRFYELLSN
                     QKTPLIYSIDQLKEMFCVADKYKRVNDFMRKVVEAAKKELDEVSPYTFEFTPLKSGRKI
                     TAIKFYPVYQPEHRDPDLEKHDLQKQVSLSWSLSSEVRSYLKNSIGYSDKEIKNNLDLF
                     ISAQSLLPDLLGELAILKGKSREKKNPKGYIINALKGKLKDKGNNHENRR"
                     /name="replication initiation protein"
     gene            980..1171
                     /locus_tag="EE52_RS23365"
                     /old_locus_tag="EE52_023370"
     CDS             980..1171
                     /locus_tag="EE52_RS23365"
                     /old_locus_tag="EE52_023370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008782581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044302092.1"
                     /translation="MKTGDRVIVPAEINGYGRDLRAIITEIEEFFGTTFVTVIFTEPCP
                     EACGRTGGVYHDFQLITE"
                     /name="hypothetical protein"
     gene            1274..1816
                     /locus_tag="EE52_RS23370"
                     /old_locus_tag="EE52_023375"
     CDS             1274..1816
                     /locus_tag="EE52_RS23370"
                     /old_locus_tag="EE52_023375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BfmA/BtgA family mobilization protein"
                     /protein_id="WP_044302090.1"
                     /translation="MNKEQESKQKRKTPLTTIAISQEYAQKLDEYIKGFALTRKDFVEL
                     AIDYFLRTGFDIRGEAFDLSPLERITNRLESSAKVMEQHNEGTEAIRQLLQAVREQTAK
                     QLPAPEIIAHAVEEKAKAESRAEEQGKKLRYVQEENDRLRNEIKDLQVYKEKAYRELCR
                     IRDEQKTFGKIHVNTEL"
                     /name="BfmA/BtgA family mobilization protein"
     gene            <1876..2142
                     /locus_tag="EE52_RS23375"
                     /old_locus_tag="EE52_023380"
                     /pseudo=""
     CDS             <1876..2142
                     /locus_tag="EE52_RS23375"
                     /old_locus_tag="EE52_023380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /name="flavodoxin family protein"
     gene            complement(2215..2709)
                     /gene="nim"
                     /locus_tag="EE52_RS23380"
                     /old_locus_tag="EE52_023385"
     CDS             complement(2215..2709)
                     /gene="nim"
                     /locus_tag="EE52_RS23380"
                     /old_locus_tag="EE52_023385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NimABCDEF family 5-nitroimidazole reductase"
                     /protein_id="WP_044302102.1"
                     /translation="MFREMRRKRQLLPTEESVAILERMTNGTLALHGDDGYPYAVPVSY
                     VYADGKIYFHSAMQGHKVDAILRNDKVSFCVVEQDEVKPAEFTTYFRSVIVFGKARILT
                     DENEKRNALNLLADKYSHGEAGMEAEMAKGFNHLLMIEITVEQMTGKEAIELTRGRNGC
                     S"
                     /name="nim"
     gene            complement(2885..3946)
                     /locus_tag="EE52_RS23385"
                     /old_locus_tag="EE52_023390"
     CDS             complement(2885..3946)
                     /locus_tag="EE52_RS23385"
                     /old_locus_tag="EE52_023390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794453.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1169 family transposase"
                     /protein_id="WP_044302100.1"
                     /translation="MNKLSRYRKLRYNQLFESENRELRLNEMGNPLEMLSQYVDFEIFR
                     PTLESALFTGERKSNAGRPPIDCVLMFKVLFLQRYYGLSDHQIEYQIVDRTSFRKFLGI
                     ECVDDVPDEKTVWKYRELLTNTGVYDKLFSEFHSFMESKGLQFNEGRIIDASFVIAPRQ
                     RNTRDENEQIKQGAGDKLWNDNPHKKCHKDVDARWTKKRDETFYGYKQHTKVEKRNKII
                     LSYDTTSAEVHDSKGFEGLLDEKDEGKDLYLDAGYVGQEEIVKQHKMNPIICEKGYRNR
                     PLTKEQKSDNRKKSKTRCLVEHVFGFEEQTMRGLVVRTVGLIRAKANVALTSLVYNISR
                     YTQIIRLKPELLG"
                     /name="IS5-like element IS1169 family transposase"
     gene            4083..4547
                     /locus_tag="EE52_RS23390"
                     /old_locus_tag="EE52_023395"
     CDS             4083..4547
                     /locus_tag="EE52_RS23390"
                     /old_locus_tag="EE52_023395"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_137572351.1"
                     /translation="MKRILFILIVMAAFFTTEAQARRIPVGEHQKITKVATLPEEYETN
                     DGLHVDLGCMYTVFEIAYIPVWTVDKGEIVCFTEQDKGSYYVIDPETLSSIKADLKIDD
                     LNSLIHIPFWDAYGGKLVVILIILLIIIYRKGKKRMKREEQAQNAESAES"
                     /name="hypothetical protein"
     gene            4684..5307
                     /locus_tag="EE52_RS24085"
     CDS             4684..5307
                     /locus_tag="EE52_RS24085"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_254695578.1"
                     /translation="MTLKGRVRGDTPRPRPPSASSMAMQTDIHQRKEKGYGKDKHQRKT
                     LQHRQRGTAQPPQQGTRLHTSGTLPPQRAMVGNEDSRRAGGHKGKVQAAHRAVHAEEGD
                     THPGGSGRNRGRHDTPATSGLCREVGGTLRNTHVPNLHAQGRGSQRLGRERGNMEAELP
                     CAHDFRLDGRGNWKNPETEQAGYGGNADHTGGMPWHGKGHFFRP"
                     /name="hypothetical protein"
     gene            5240..6067
                     /locus_tag="EE52_RS24090"
     CDS             5240..6067
                     /locus_tag="EE52_RS24090"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_254695579.1"
                     /translation="MAEMQTILAECLGMERGISSDRKHLSAIQYKNMVEAEKAAQIEKE
                     CSQMEDERNAIEEKVKQAGQKLEDTQKKLKEAAAEIKVEKLKGAAADTGTALLKAGTTV
                     IDATASLFNSGKVKRQEQEIAELKRENYELEMKSQNLQSNLRTANAQNEREREATRRAV
                     RTGEERLKPITNLFPCMENAKENIEELREMGVRDNDIRLLLTGKEITYSGFLYDRERRK
                     KHQVKNVKINIAKSKAGNTTIWLNDFHFKEFFKQLWQKLQKVLGIDKGRGFHL"
                     /name="hypothetical protein"
     gene            complement(6062..6400)
                     /locus_tag="EE52_RS23400"
                     /old_locus_tag="EE52_023405"
     CDS             complement(6062..6400)
                     /locus_tag="EE52_RS23400"
                     /old_locus_tag="EE52_023405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin RelE"
                     /protein_id="WP_044302095.1"
                     /translation="MSFEIIPTPDFSRSLKVLAKRHRSLKQDMSVFVSSLKENPLQGTE
                     IAPNIRKVRLTITSKGKGKSGGARVITYLMAVSEDAGDVYLIDIYDKSDYDTVDVSVIK
                     KMIADLGL"
                     /name="toxin RelE"
     gene            complement(6390..6692)
                     /locus_tag="EE52_RS23405"
                     /old_locus_tag="EE52_023410"
     CDS             complement(6390..6692)
                     /locus_tag="EE52_RS23405"
                     /old_locus_tag="EE52_023410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system prevent-host-death
                     family antitoxin"
                     /protein_id="WP_052510138.1"
                     /translation="MLKYINLDAVSVSIFIFAKKIKVMQVVSAREFRANQGKYLIKANE
                     GQSVLLTSRYGNFKIVPISEEDNLTTRICRGLQQVKLIEEGKLPRRTIEDLLDEL"
                     /name="type II toxin-antitoxin system prevent-host-death
                     family antitoxin"
     ARG             complement(2219..2709)
                     /name="nimD"
                     /gene="nimD"
                     /gene_name="5-nitroimidazole reductase NimD"
                     /tool="amrfinderplus"
                     /drug_class="NITROIMIDAZOLE"
                     /identity="98.78"
                     /coverage="100.0"
     Typing          4791..6064
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaatag taaacaaaga cctcattcaa agttatatta tgactaccgc caaatatgac
       61 tttaacgtgt acgaaaaacg gataatttac cgcctcgtag agatggctca aggggagcta
      121 caaggcataa agttcgctaa taactgtcag aaaatagagc atgacctttt cagttgcata
      181 aacattacta tgcctatcgc ttgccttctc aatggagaag aagataagaa ctacgcaaga
      241 gtaaaggaag ccttacaatc gctccaacga aaaatatttg agtatgaaga tgatgaagtt
      301 tggcagagta tatcaatcat cgcacttccc tacattcgga aacgttcttc tgtgatttct
      361 ttccaagtgc atccacgtgt atgggattgc attcttaatt tctccaaagg ctatcggaaa
      421 tatgagctaa aagccgccat gagcttcaag agccaattct cgatgcgctt ttatgagcta
      481 ctcagcaatc aaaaaacacc attaatctac tcaatagacc aactgaaaga aatgttttgc
      541 gtggctgaca aatataaacg cgttaacgat tttatgcgca aggttgtaga agctgcaaaa
      601 aaagaattag acgaagtaag cccatataca tttgaattca cccctctcaa atcgggacga
      661 aagataactg ctataaagtt ttacccagtg taccaaccgg aacatagaga ccctgattta
      721 gaaaaacacg atttgcaaaa gcaagtttct ctaagctggt ctttatcttc agaagttcgt
      781 agctatttga agaactcgat agggtattcc gacaaagaaa taaaaaacaa cttggattta
      841 tttatatcgg cacaaagttt acttcctgat ttactcggag aactcgctat acttaaagga
      901 aagtcaaggg aaaagaaaaa cccaaaagga tatattatca atgcactcaa agggaaacta
      961 aaagacaaag gcaataatca tgaaaacagg agatagagtt atcgtaccag ccgaaattaa
     1021 cggatacggc agggatttac gggctatcat tacagagata gaagaatttt tcgggacaac
     1081 attcgtaacg gtgatattta cagaaccatg cccggaagcc tgcggaagaa cagggggtgt
     1141 ttaccatgat ttccagctaa taacagagtg atttattttg atataattgc aataaaacgc
     1201 aataatattc attataaatt gcaataacaa ggaaaacaac tacctttgta acaagatata
     1261 gcattttaca tcaatgaaca aagaacaaga aagtaagcaa aagaggaaga cgcccttaac
     1321 gaccatagcg ataagccaag aatacgccca aaagctggac gaatatataa aggggtttgc
     1381 ccttacacga aaggactttg tagagctggc tatcgactat ttcctacgta ccgggtttga
     1441 catacgtgga gaagcgtttg acctctctcc attggagcgc ataaccaaca ggctggaaag
     1501 ttcagctaag gtcatggaac agcacaacga aggaacagag gcgatacgcc agctattaca
     1561 ggcagtccgg gaacaaaccg ctaagcagct tccggctcct gaaatcattg cccatgccgt
     1621 cgaagaaaaa gcaaaggctg aaagcagggc tgaagaacaa ggaaagaagc tccgctacgt
     1681 acaggaagaa aacgaccgat tacgtaacga aatcaaagac ttgcaagtct acaaggaaaa
     1741 agcttaccgg gaactgtgcc ggatccgtga tgaacaaaag actttcggaa aaatacatgt
     1801 gaatacagaa ttatagtgga tttttgttca tagaaacaga aatttaacac agaaaaggta
     1861 gcaatgataa ataaaggagc ttccgctaca ttcgagcaac tgaaccaata tttcaccatc
     1921 tgcaatatgc cgattgttgc ttcacagtac tggaactcgg ttcacggatt tactccggat
     1981 gatgtacgca aggataaaga aggactgcaa accatgcgta cattgggaca aaatatggct
     2041 tggctgttga agtgcattga aagtggcaag cagaacggaa taaagaaacc tgaatatgaa
     2101 gcaagggtac gcacccactt catacaagac aaatatgaat aaccataaac aaagtagaaa
     2161 agctgtttcg gcggacgcga aacagctttt tctccgtcag tcagccattc tttgtcaaga
     2221 acatccgttt cttccccttg tcagttcgat ggcttctttt ccggtcatct gctctactgt
     2281 gatttctatc atcagcaaat gattgaaccc tttggccatt tcagcctcca tgcccgcttc
     2341 gccatgcgaa tacttgtcgg ccagcaggtt taaggcattt cgtttttcgt tctcgtcggt
     2401 cagtatgcgg gccttgccaa agactatcac gctccgaaaa taggtggtaa actcggccgg
     2461 cttgacctca tcctgctcca ctacgcagaa cgagaccttg tcattccgca ggatggcatc
     2521 cactttgtgc ccttgcatgg cactgtggaa gtaaattttg ccatcggcat atacataact
     2581 gacagggacg gcatacggat agccgtcatc cccatgaaga gccaacgtcc cgtttgtcat
     2641 ccgttcaaga atggcaacgc tttcttctgt tggcaacaat tgccgcttac ggcgcatttc
     2701 tctaaacata ttcttttggt gagttctatc aattcaattt atcctcttgt atcagacagc
     2761 agaactcttt aactgcccaa tttgtgagtt ttagtctttc aaatttagcg aatattttct
     2821 acccccccaa tttttacaat aattaacttg tgtttgttgt aaaaattgga tgtgcaggca
     2881 taattcaccc caacaactca ggtttcagcc tgattatttg cgtgtaacga ctgatgttat
     2941 acacaagact ggtgagtgct acattggctt tagcacgaat aagccctact gtacgcacca
     3001 caagtccacg catggtttgt tcctcaaacc cgaatacatg ctcgacaagg caacgtgtct
     3061 tggatttttt cctattgtct gatttctgct ccttggtaag cggacggtta cggtagccct
     3121 tttcgcaaat tatcggattc atcttatgct gttttacaat ctcctcttgt ccgacataac
     3181 cggcgtccaa atacaagtcc ttgccttcgt ctttttcatc cagcagtcct tcaaagcctt
     3241 tggaatcatg cacttctgcc gacgtggtat cataagaaag tatgatctta ttgcgtttct
     3301 caactttagt atgctgcttg tagccgtaaa aagtctcatc acgcttcttt gtccagcggg
     3361 catctacatc cttgtggcac ttcttgtggg gattgtcatt ccacaactta tcacccgctc
     3421 cctgttttat ctgctcattt tcatcacggg tgttacgttg gcgaggggca ataacaaaac
     3481 tggcatcaat gatgcgaccc tcattgaatt gcagaccctt actttccatg aaactatgaa
     3541 attccgaaaa aagcttgtca taaacgcctg tatttgtcag gagttcgcga tacttccaca
     3601 ccgtcttctc gtcaggaaca tcgtcaacac attcaatacc aagaaacttg cggaaactcg
     3661 tacggtcaac tatctgatac tctatctgat ggtcactcaa accataataa cgctgaagaa
     3721 acaagacctt gaacatcagc acacagtcta tcggcggacg accggcatta cttttgcgct
     3781 ctccggtaaa aagggctgat tcaagagtag gacgaaagat ctcaaaatca acatactgag
     3841 acaacatttc aagaggattg cccatttcat tcaaacgcaa ttcacgattc tctgactcaa
     3901 atagttgatt ataacgaagc ttacggtatc gggataactt gttcattata aaattcttat
     3961 tttctgcaaa tttacgctct tggaaccata taaacaactg tactacaata ttttaattta
     4021 tagaactcct ctaaaatcaa tacttttgcg tagatgtaaa tgaactttaa aaggatatga
     4081 taatgaaaag gattctattc attttgatag taatggctgc attctttaca acagaagcac
     4141 aagcccgtag aatacctgtc ggtgaacatc agaaaatcac taaggtagcg actttacctg
     4201 aagaatatga aaccaacgat ggtttacatg tggacctcgg atgtatgtac acggtgtttg
     4261 aaatagcata catccccgta tggactgtag ataagggaga gatagtctgt tttactgaac
     4321 aggacaaggg ttcgtattat gttatagacc ctgaaacatt atcatctatt aaagcggact
     4381 taaaaataga tgatttaaat tcattgatac atataccttt ttgggatgct tatggaggta
     4441 aattagtcgt tatattgata atattactta ttatcatata ccgaaaaggg aaaaagagga
     4501 tgaaaagaga agagcaagct caaaatgccg aaagtgccga aagttaaaag gatacgcact
     4561 tatcaagcat tcaatcctat ttatcgttcc taaatgtatg cttttgcttt gcactttacc
     4621 tttttgaata tttgcggatt tcgaccgaat ggcttaactt tgcttgcgag gttatagcac
     4681 actatgaccc taaagggtcg tgtgcgaggg gacacccctc gaccccgtcc gccatcggca
     4741 agctcgatgg cgatgcaaac ggatatccac caacgaaaag agaaaggtta tggcaaagac
     4801 aagcatcaac gtaagaccct gcaacatcgg cagcgcggaa cggcacaacc tccgcagcaa
     4861 ggaactcgac tacatacgtc cggaactctc ccaccgcaac gagcaatggt cggaaatgaa
     4921 gatagcagac gtgctggagg acataaggga aaagtacagg cagcacaccg ggcagtccat
     4981 gcagaagaag gcgacaccca tccgggaggg agtggtcgta atcgaggacg gcacgacact
     5041 ccggcaactt caggactttg ccgggaggtt ggaggaacgc ttcggaatac acacgttcca
     5101 aatctacacg cacaaggacg agggagccag cgtttgggac gggaacgcgg aaacatggaa
     5161 gccgaactac catgcgcaca tgattttcga ctggacggac ggggaaactg gaagaacccg
     5221 gaaactgaac aagcaggata tggcggaaat gcagaccata ctggcggaat gccttggcat
     5281 ggaaaggggc atttcttccg accgtaagca cctttccgcc atccagtaca agaacatggt
     5341 cgaagcggag aaagcggcgc aaatcgaaaa agaatgcagt cagatggagg atgagcgcaa
     5401 cgccatagag gaaaaagtga aacaggcagg acaaaagctg gaggacaccc aaaagaagct
     5461 caaggaagca gcagccgaaa taaaggtgga aaagctgaaa ggggcggcag ccgacacggg
     5521 aacagccttg ctgaaagccg ggacgaccgt catcgatgcg acagccagcc ttttcaactc
     5581 cggaaaggtc aaacggcagg agcaggagat tgcggaactg aaaagggaga attacgaact
     5641 agagatgaaa agccagaacc tccagagcaa cctgcgtaca gcaaacgccc aaaacgaaag
     5701 ggaacgggaa gccacacgca gggcagtcag aaccggagaa gaaagactga agccgataac
     5761 caacctgttc ccctgtatgg aaaacgccaa agagaacatc gaggaactcc gggaaatggg
     5821 tgtccgagac aacgatatcc ggctcctgct cacaggaaaa gagataacct attcaggatt
     5881 cctgtacgac cgagaacgca gaaaaaagca ccaggtcaag aacgtgaaga taaacattgc
     5941 taaaagcaag gctggcaaca cgaccatttg gctgaacgat tttcacttca aggagttctt
     6001 caaacagcta tggcaaaagc tgcaaaaggt tttaggaata gacaaaggca ggggattcca
     6061 tttatagtcc caaatctgca atcatttttt taataacgga cacatctact gtgtcatagt
     6121 cggatttatc atagatgtct atcaaataca cgtcccccgc atcttcagac acagccatta
     6181 agtaggtaat gacccttgcg cctcctgatt tacctttccc cttagatgta attgtcagtc
     6241 tgacttttct gatgttgggg gctatttctg tcccttgcag aggattctct ttcaaacttg
     6301 acacaaatac agacatatcc tgtttcaaag acctatgtcg tttggcgaga actttgaggg
     6361 aacgggaaaa atcaggtgtc ggtataattt caaagctcat cgagtaagtc ctctatagtt
     6421 ctacgtggaa gttttccttc ttctatcagc ttcacttgct gcaatcccct gcaaatgcgt
     6481 gtggttaaat tatcttcttc cgatatagga acaattttga aattaccgta cctgcttgtg
     6541 agcaatacgg attgtccttc gttggcttta attaggtatt tcccctgatt agccctaaat
     6601 tctcttgctg aaactacttg cataaccttt attttttttg caaagataaa aatagatacc
     6661 gaaacggcat ccaaattgat gtattttaac ataaattgca atcactattt taccacatca
     6721 tgaaagtggc ggcttattga aaattagcat agcacacctg aaaaacccga atttggtgat
     6781 tacaaccgcc aaattcatac ctaatttagc ggttagataa tatacataaa caaaatgata
     6841 catattttaa cgcgccagaa aagtcgttcc ccaaaaatcg ctgcgctagc tcttgctttg
     6901 cttcctcaaa cattcaaaaa gtcgtttttc tttttccgaa agttgaaaat cagccatttc
     6961 gttaaaaatc tatgacttgt tttaaagcat tgcttattat atgcttatca gcttctcgaa
     7021 actccgaata aaatacctta ttatcagcct tttacaaaca ataaaacgag ccaaactacg
     7081 actttttgaa tactaactac gactttttga atgctaattc aagacttaac tacgactttt
     7141 tgaatactat ttcaaaatat atacgacttt tatttttata agtcgttttt agtgcttata
     7201 tttgcacaaa atactgataa t
//
