LOCUS       NZ_CP036541             5594 bp    DNA     circular CON 25-JUL-2023
DEFINITION  Bacteroides fragilis strain DCMOUH0017B plasmid pBFO17_2, complete
            sequence.
ACCESSION   NZ_CP036541
VERSION     NZ_CP036541.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02730070
            Assembly: GCF_000710375.2
KEYWORDS    RefSeq.
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Sydenham,T.V., Hasman,H., Wang,M., Soki,J., Nagy,E. and
            Justesen,U.S.
  TITLE     Identification of resistance genes in three multidrug-resistant
            Bacteroides fragilis isolates by whole genome sequencing
  JOURNAL   (in) ? (Ed.); THE 24TH EUROPEAN CONGRESS OF CLINICAL MICROBIOLOGY
            AND INFECTIOUS DISEASES; ECCMID 2014, Barcelona, Spain (2014)
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Sydenham,T.V., Hasman,H. and Justesen,U.S.
  TITLE     Complete genome assembly of MDR B. fragilis
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5594)
  AUTHORS   Sydenham,T.V., Hasman,H. and Justesen,U.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2019) Department of Clinical Microbiology, Odense
            University Hospital, J.B. Winsloews Vej 21,2, Odense C 5000, Denmark
REFERENCE   4  (bases 1 to 5594)
  AUTHORS   Sydenham,T.V., Hasman,H., Soki,J. and Justesen,U.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2014) Department of Clinical Microbiology, Odense
            University Hospital, J.B. Winsloews Vej 21,2, Odense C 5000, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: OUH_BF17_3.manual
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/25/2023 04:18:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,710
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 29 of 82
            Pseudo Genes (incomplete)         :: 53 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 16 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036541.1.
            Bacteria and source DNA available from Ulrik S. Justesen.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="DCMOUH0017B"
                     /isolation_source="Blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="pBFO17_2"
                     /geo_loc_name="Denmark: Odense"
                     /lat_lon="55.3958 N 10.3886 E"
                     /collection_date="2006-06-06"
                     /collected_by="Ulrik Stenz Justesen"
     gene            1..771
                     /locus_tag="EC81_RS24920"
     CDS             1..771
                     /locus_tag="EC81_RS24920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005799241.1"
                     /translation="MKKVMFMFISLLTVLGVCSCNDNEVIEPVVSDSVSDMEVLSRFVD
                     VNEITNEYYFNENKKTRALSYVTGSDWQDLEKVSPLSIEKYKNNLQVLNAQVASAISNP
                     NTAYVVFSVNGKTLVKKVKEDANFDFSVFRDVVTETRAVLPSLSINGGSQSTTGVFYDS
                     SRTLKMQVDLNASIQNNYYFFEVLNPNAKPSPDDNITTPESVAFSGTGPLWSNTFTWTS
                     YWDANVPGQGFKWEFKGKGTTPSFGFIANCTFSR"
                     /name="hypothetical protein"
     gene            complement(841..2091)
                     /locus_tag="EC81_RS24630"
                     /old_locus_tag="EC81_024635"
     CDS             complement(841..2091)
                     /locus_tag="EC81_RS24630"
                     /old_locus_tag="EC81_024635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBU/pMV158-type mobilization protein"
                     /protein_id="WP_005799237.1"
                     /translation="MAKTCIRVEACNIGSSERHNLRSKELDYIRPELTHRNEQWVECSI
                     AEVHRDITEKYKEATGQGLQKKATPIREGVIVISEETTIQQLQDLAEKLEERFGVHAFQ
                     IYTHKDEGANVWDGKEEAWKPNYHAHMIFDWTDGHTGKTVKLNRHDMAEMQTITAECLN
                     MERGVSSDKKHLSAMQYKNKMETEKAEQLQKDIEQLNRAYTAGTEKITTVQKELSTAQK
                     ELNSMKTDIHINEAKEAAAKAGKTVFNAVTSVFNVGEVKRQAKEIETLKKENYNLSFKN
                     QNLEGQLRTNNIEMRRVQETTDRQIKAEASKLKPITNLFPEMENAQENIEELQTMGIQN
                     KDIRQLLIGKEIYYTGNLYDKDKRKSYQVKNVKIDISKSQNGITTIWLNNIHFKNFLKE
                     LWNTLQKTLDNGNWLHR"
                     /name="NBU/pMV158-type mobilization protein"
     gene            2497..2745
                     /locus_tag="EC81_RS24635"
                     /old_locus_tag="EC81_024640"
     CDS             2497..2745
                     /locus_tag="EC81_RS24635"
                     /old_locus_tag="EC81_024640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_005812783.1"
                     /translation="MVTNIIQIGNSKGIILPSEVLKQLRLSLKSAVSISLDGNNIVIKA
                     QPRQGWAEAAKRAHENGDDELLIPDVFEDEKFEDWTW"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            2739..3059
                     /locus_tag="EC81_RS24640"
                     /old_locus_tag="EC81_024645"
     CDS             2739..3059
                     /locus_tag="EC81_RS24640"
                     /old_locus_tag="EC81_024645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799262.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003723 - RNA binding [Evidence IEA]; GO:0004521 - RNA
                     endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_005799262.1"
                     /translation="MVEQYEVYWVELDPTRGGEMAKTRPCVVVTPSDLNMYLTTVVIVP
                     ITSTIRNYPYRVLCSVAGREGEIATDQIRTVDKSRLKRKIGDLNFSEIKRLREVFQQMF
                     CE"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            3096..3494
                     /locus_tag="EC81_RS24645"
                     /old_locus_tag="EC81_024650"
     CDS             3096..3494
                     /locus_tag="EC81_RS24645"
                     /old_locus_tag="EC81_024650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005799260.1"
                     /translation="MKPTDYIEWDNLKDIPFFLCQVVEDREKQDLDIYYLGKRVLHDYD
                     HVGHYLRTAVILFRRVKSRTADWVNLRNLWTLRNCVRENYNHGIGMNDLIFGENFDGDN
                     LDTLTPLTKKRFDFLCKRIKELDPYATI"
                     /name="hypothetical protein"
     gene            4136..5038
                     /locus_tag="EC81_RS24655"
                     /old_locus_tag="EC81_024660"
     CDS             4136..5038
                     /locus_tag="EC81_RS24655"
                     /old_locus_tag="EC81_024660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_229101267.1"
                     /translation="MKAYMDAHVCSNVYTVQGGKAHSHYCHRAFCPICQRIQTAQNVKK
                     FLPVMKYLASEGREFYFVTLTLQNCVTDDARVLRDFMRRCNRMWADGIRTKHKFRSLGI
                     SGVIKKECTYHVVKKDLFSFHYHFHIIVDSLEAAKYVVAQWKKLHGSTVADARFQKYKK
                     IEDFENAAIEVFKYASKASVSKSKGRDGKEKIQINYKALDMIYTAMHGMQRMSSFGQFR
                     TMIGDEALNDFRDEDLVLNIEGLNVEDGCYTWYMSVKDKVADWYNMETGEALCCYKVTR
                     KDELLQDIYLDEDIFPNSG"
                     /name="protein rep"
     gene            complement(5042..5503)
                     /locus_tag="EC81_RS24620"
                     /old_locus_tag="EC81_024625"
     CDS             complement(5042..5503)
                     /locus_tag="EC81_RS24620"
                     /old_locus_tag="EC81_024625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005812785.1"
                     /translation="MNRIIHISLLISTVSLFLISNTGCVEKQGYYNHGEESIISLICDI
                     TWAGKKTTDENGSVWQGTYKFNKNGTYTRTNIEIDKQGNKKEANIYGQWSFGDPSFSTI
                     YFGGEHYWDIDELTKNKFSFYDRSGKFGDPFMNREYIELTPYQENNTTN"
                     /name="hypothetical protein"
     Typing          3990..5038
                     /name="rep_cluster_870"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.81"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(845..2091)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaaaaaag taatgtttat gttcatcagt ctactgactg ttttgggtgt ttgttcgtgt
       61 aatgataatg aagtaataga acctgtggtt agtgattctg tatctgatat ggaggtctta
      121 tctcgttttg ttgatgtaaa tgagattaca aatgaatact atttcaatga gaataagaaa
      181 acgagggcgt tgtcttatgt aacaggttcg gattggcagg atttagaaaa agttagtcct
      241 ttgagtattg agaaatataa gaataacctg caggttttaa atgctcaagt ggcaagtgct
      301 atttcaaatc ctaataccgc ttatgttgtt ttttctgtaa atggaaagac tttggttaaa
      361 aaggtgaagg aggatgctaa ttttgatttt tcagtattta gggatgttgt aacggaaacg
      421 agggctgttc tcccttcttt gagtattaat ggaggttctc aaagcactac cggagttttt
      481 tacgattcca gtcgtacttt aaagatgcaa gttgatttga atgcttcaat acagaataat
      541 tattatttct ttgaagtatt aaatcctaat gctaaaccaa gtccggatga taatataacg
      601 acacctgaaa gtgttgcttt tagtggaact ggaccgttat ggagtaacac tttcacttgg
      661 acttcgtatt gggatgcaaa tgtacctggt caaggtttta aatgggagtt taaaggaaaa
      721 ggtactactc cttcttttgg ttttatagct aattgcacct ttagtcgtta aaaaatgaac
      781 gggatttaat ttgaattgca cttctcaaaa aagagaagtg caattctttt tagtgtcttt
      841 ctatcggtgt agccagttac cgttatctaa agtcttttgt agtgtattcc aaagttcttt
      901 aagaaagttt ttaaagtgga tgttatttag ccaaattgta gtaataccat tctgactttt
      961 ggagatatct attttcacgt ttttaacttg gtatgatttt ctcttgtctt tatcgtaaag
     1021 gtttccagtg tagtatattt cctttccaat aagaagttgt cggatatctt tattttgtat
     1081 tcccatagtt tggagttcct ctatattttc ctgtgcattt tccatttccg gaaaaaggtt
     1141 ggttatgggt tttagtttgg atgcttctgc ctttatctgt ctgtcggttg tttcctgtac
     1201 ccttcgcatc tcgatgttgt tggttcttaa ttgtccttct aaattttgat tcttgaatga
     1261 gaggttataa ttttcttttt tgagggtttc aatttctttg gcttgtcgtt ttacttcccc
     1321 tacattaaag acgcttgtaa cggcattaaa tacggttttt ccggcttttg cggcagcttc
     1381 tttggcttcg ttgatgtgta tgtcggtttt catgctgttt agctcttttt gtgccgtact
     1441 aagttccttt tggactgtgg tgattttctc ggttccggca gtgtatgccc tgtttagttg
     1501 ctcgatgtct ttttgaagtt gttcagcttt ctctgtttcc attttgtttt tgtattgcat
     1561 ggccgacaag tgtttcttgt ctgaagaaac tccacgttcc atgttgagac actctgctgt
     1621 tatagtctgc atctccgcca tgtcgtgtct gttcagcttt acagtttttc ctgtatggcc
     1681 gtccgtccag tcaaagatca tgtgcgcatg atagttcggt ttccatgctt cttcctttcc
     1741 gtcccatacg tttgcgcctt catccttatg ggtgtagatt tggaaggcgt gcactccaaa
     1801 gcgttcttcc agtttttcgg ccaagtcttg aagctgctgg attgtggttt cctcgctgat
     1861 tactattacc ccttcccgga ttggggtagc tttcttctgt aatccttgtc cggtggcttc
     1921 cttgtatttt tccgttatgt cccggtgtac ttcggcaatg gaacattcta cccactgttc
     1981 gttgcggtgt gtgagttccg gacggatgta gtcgagctcc ttgcttctta agttgtgacg
     2041 ttccgagctg cctatattgc aggcttccac tctgatacat gtctttgcca tatctttagg
     2101 ttttaggtgt gtatgtctgt ttagaagatg tggcgtgaaa cgtcacatcg cacggggtcg
     2161 aggggacacc cctcgcacac aaccccttta ggggttatag tgtgctataa ccttttgcaa
     2221 aggtaagcct tccggatggg atttgcaagc gctctgccat gtgttccgga tgtgcttggg
     2281 aacagaagcc ggacaaaagg gaaaaggaca caaggcacaa taaaaaatca tcggtcgggg
     2341 agcgctgcac ggcaaggggg tgcaccaaca ctcccgacga gtgcagccat tgacagggtt
     2401 ctcggtgaaa atggaaaaaa gttgtttttc cttttgttat tgttataact ttacatatat
     2461 ttgtatcgtg ataatcaatt ataaagttat aacattatgg taacgaatat tattcagatt
     2521 ggaaacagca aggggataat acttccttcc gaagtattga aacagttgcg tttgtccttg
     2581 aaatcggctg tgagcatttc tttggacggg aacaatattg tgataaaggc acaaccacgg
     2641 cagggatggg cggaagctgc caaacgtgcc catgagaatg gggatgatga gttgcttatc
     2701 cctgatgtgt ttgaggatga aaagtttgag gattggacat ggtagaacag tatgaagtgt
     2761 attgggtaga gttagaccct acccgtgggg gtgaaatggc aaagacacgg ccatgtgtag
     2821 ttgtcactcc ttcagatttg aatatgtacc ttacaacagt agtcattgtt ccgataacct
     2881 caacgataag aaattatcct taccgggttc tgtgttctgt agccgggcgt gaaggagaga
     2941 tagccactga tcaaattcgc acggtggata aaagccggct gaaaagaaag attggagatt
     3001 tgaatttcag tgagataaaa aggttacgtg aagtgtttca gcaaatgttc tgtgaataat
     3061 ggaaagtacg gaaagaaaaa tgcaaataag acattatgaa accaactgac tacatcgaat
     3121 gggataacct gaaggatatt ccattcttcc tctgtcaagt agtcgaggac agagaaaaac
     3181 aagatttgga tatttactat ttgggcaaac gtgtcttgca cgattacgac catgtggggc
     3241 actatttgcg gactgccgtt atcttgttcc gcagggtaaa gagccgcaca gcagactggg
     3301 taaacctgcg taacctttgg acgctgcgca actgtgtgcg tgagaactac aatcacggca
     3361 taggaatgaa cgaccttatt tttggggaga atttcgatgg agataatctc gatacgctta
     3421 caccacttac aaagaaacgc tttgattttt tgtgtaagcg gattaaggaa cttgacccat
     3481 atgcaactat ttaacactcc attgagtgaa ggcagggttt gtctttgtgt gctccccctt
     3541 tgggggacgg gagggggata agaaaaaaaa taccaccgga gatgtcggat atccgtcaat
     3601 tcactccggt gttttttttg caactctccc gtatcgctcg gcaagggttg tctttgtgta
     3661 cggcaaagat agtttttttt ccggaagtat ctttgtctgt tgtggattta gtagtgtatt
     3721 tgtgtcagaa ttgcgcagcc gggggaatgg gggcgagccc ccatactaga taaactagaa
     3781 agtttcgtgg taaatgcttt ttgacttgtt atatgtaggt tttttgaatg atgtttggcg
     3841 gtttggaatg aattacctac ttttgggctg agaaaaaacg aaagcccgaa ggcagtaacc
     3901 aacgggcttt gtattttaag aattagcaat cgtttgtgtt cgttcttggc ggggcggata
     3961 ccaaacgaaa gccacatcaa aatttcatat gtgcaaagat agaacaaata taaccaactg
     4021 caaagaaagt ccgttaaaaa acggttctga tagtcttaaa aaaaaggcta aagtaaaaat
     4081 catatcccat aattttactc gaaaactttc ggaaaagaat ccggatagta agctgatgaa
     4141 agcatatatg gatgcccatg tgtgttcaaa tgtttatact gtgcagggag gtaaggcgca
     4201 ttcccattat tgtcatcggg ctttttgtcc tatttgtcag cgtattcaaa cggcacaaaa
     4261 tgtaaagaag ttccttccgg ttatgaagta tttggcttcg gaaggcagag agttttattt
     4321 tgtgacgctt accttgcaaa attgtgtaac cgatgatgca agggtattgc gagatttcat
     4381 gcgtagatgt aaccggatgt gggctgatgg tataaggacg aaacacaagt ttcgttcact
     4441 tggtatttcg ggggtgatta agaaggagtg tacctatcat gtagtaaaaa aggatttgtt
     4501 ttcgttccat tatcattttc atattattgt tgattcattg gaagctgcaa aatatgtagt
     4561 agcccaatgg aagaaattgc atggtagtac ggttgctgat gcccgttttc agaaatataa
     4621 gaagatagag gattttgaaa atgcagcgat agaggttttt aagtatgctt caaaggcttc
     4681 tgtctcaaaa tcgaaaggtc gtgacggtaa agaaaagatt cagataaatt ataaggcttt
     4741 ggatatgatt tatactgcca tgcatgggat gcagcgcatg agtagtttcg gacagttccg
     4801 caccatgatc ggagatgaag cgttgaatga ttttagagat gaggatttgg ttcttaatat
     4861 tgagggattg aatgttgaag atggttgtta tacttggtat atgtctgtga aagataaggt
     4921 ggctgattgg tataatatgg aaactgggga ggcattgtgt tgttataagg tcacccggaa
     4981 ggatgaactt ttgcaggata tttatttaga tgaggatatt tttcctaata gtgggtagtt
     5041 gttagtttgt tgtattgttt tcttggtatg gtgtgagttc tatgtattct ctattcatga
     5101 aaggatcgcc aaattttcca cttctgtcat agaaggaaaa tttgttttta gttagctcgt
     5161 ctatgtccca gtaatgttct ccaccaaaat aaatggtact gaaacttgga tcaccgaatg
     5221 accattgtcc gtagatattg gcttcttttt tatttccttg tttgtcgatt tctatatttg
     5281 tacgggtata tgttccgttt ttattgaatt tgtatgtacc ttgccagaca ctgccgtttt
     5341 cgtctgttgt ttttttacca gcccatgtaa tatcacaaat taatgagatt atgctttctt
     5401 ctccatggtt gtagtatcct tgtttttcaa cacatccggt gtttgaaata agaaaaagtg
     5461 atactgttga aataagtaaa gagatatgta ttattctatt catagttaaa tatttaatat
     5521 tcaatgcaaa aataggagaa attttatatt tttgggcgaa acctttattt attaacttta
     5581 aaaaaggagg attt
//
