LOCUS       NZ_CP036445             9293 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain ABFPV plasmid tig00001273_pilon,
            complete sequence.
ACCESSION   NZ_CP036445
VERSION     NZ_CP036445.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03455989
            Assembly: GCF_004322955.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Conlan,S., Thomas,P.J., Mullikin,J., Palmore,T.N., Frank,K.M. and
            Segre,J.A.
  TITLE     Whole genome sequencing of Klebsiella pneumoniae ABFPV
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9293)
  AUTHORS   Segre,J.A. and Mullikin,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2019) NIH Intramural Sequencing Center, 5625
            Fishers Lane, Rockville, MD 20852, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.8
            Genome Coverage                   :: 150x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 22:33:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,516
            CDSs (total)                      :: 5,393
            Genes (coding)                    :: 5,282
            CDSs (with protein)               :: 5,282
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 49 of 111
            Pseudo Genes (incomplete)         :: 64 of 111
            Pseudo Genes (internal stop)      :: 20 of 111
            Pseudo Genes (multiple problems)  :: 19 of 111
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036445.1.
            Bacteria and source DNA available from Karen Frank, Department of
            Laboratory Medicine, National Institutes of Health Clinical Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ABFPV"
                     /db_xref="taxon:573"
                     /plasmid="tig00001273_pilon"
                     /geo_loc_name="USA"
                     /lat_lon="38.984652 N 77.094709 W"
                     /collection_date="2014"
     gene            join(7486..9293,1..157)
                     /locus_tag="WN05_RS28120"
                     /old_locus_tag="WN05_28125"
     CDS             join(7486..9293,1..157)
                     /locus_tag="WN05_RS28120"
                     /old_locus_tag="WN05_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            157..888
                     /locus_tag="WN05_RS28125"
                     /old_locus_tag="WN05_28130"
     CDS             157..888
                     /locus_tag="WN05_RS28125"
                     /old_locus_tag="WN05_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            895..1425
                     /locus_tag="WN05_RS28130"
                     /old_locus_tag="WN05_28135"
     CDS             895..1425
                     /locus_tag="WN05_RS28130"
                     /old_locus_tag="WN05_28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1453..1632)
                     /locus_tag="WN05_RS28135"
                     /old_locus_tag="WN05_28140"
     CDS             complement(1453..1632)
                     /locus_tag="WN05_RS28135"
                     /old_locus_tag="WN05_28140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1701..2078
                     /locus_tag="WN05_RS28140"
                     /old_locus_tag="WN05_28145"
     CDS             1701..2078
                     /locus_tag="WN05_RS28140"
                     /old_locus_tag="WN05_28145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(2140..2439)
                     /locus_tag="WN05_RS28145"
                     /old_locus_tag="WN05_28150"
     CDS             complement(2140..2439)
                     /locus_tag="WN05_RS28145"
                     /old_locus_tag="WN05_28150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2429..2692)
                     /locus_tag="WN05_RS28150"
                     /old_locus_tag="WN05_28155"
     CDS             complement(2429..2692)
                     /locus_tag="WN05_RS28150"
                     /old_locus_tag="WN05_28155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(2998..3417)
                     /locus_tag="WN05_RS28155"
                     /old_locus_tag="WN05_28160"
     CDS             complement(2998..3417)
                     /locus_tag="WN05_RS28155"
                     /old_locus_tag="WN05_28160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            3819..5504
                     /locus_tag="WN05_RS28165"
                     /old_locus_tag="WN05_28170"
     CDS             3819..5504
                     /locus_tag="WN05_RS28165"
                     /old_locus_tag="WN05_28170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5514..5771
                     /locus_tag="WN05_RS28170"
                     /old_locus_tag="WN05_28175"
     CDS             5514..5771
                     /locus_tag="WN05_RS28170"
                     /old_locus_tag="WN05_28175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            5856..6005
                     /locus_tag="WN05_RS28175"
                     /old_locus_tag="WN05_28180"
     CDS             5856..6005
                     /locus_tag="WN05_RS28175"
                     /old_locus_tag="WN05_28180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..9293
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6109..6687)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          158..885
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 ccggatggcg ttcagcagtc cgctgcgggt tgagcgccgg gaaatcccgt tgcagcgttt
       61 cccggtgctg gcgaaaaccg atcccctggc ggagttccgg cccccggccg acggcggcgg
      121 gccgaaagcg gatgatgatt ttgcggggat ttactgatgg cgctggagct gtacaacgtc
      181 agccacgcga aacgacaggc gcggaacgcg gagaaaaccc ggctgaccct gcgctggttg
      241 cgggaagagc tgtgttccac ggcggagctg gttgcgcggc ggctggggat tgcggcggtg
      301 cagccggtct accgcttcct cgacagcctg gtggcgaaag ggctgctggt ccggaccaaa
      361 tatccggtgg acggccgtca ggtcagcgtg tgggggctga cgccgcatgg cgtggcgttc
      421 tcgttcgacg aggacgaacc gctggcggat gttattccgt ttcagccgtc aagggtgtcg
      481 gcagcacatc tgccgcaccg gctggccgtg caatccctgc gccttgatat ggaagccaga
      541 ggggccaccg gctggcgcat tctgcaccgt ctgagtctta aaggcatgaa agtgccggac
      601 gccctggcgg agctggacgg taaaacggtg gcgtttgagg tggagcgcac cgtcaagagc
      661 cgtcgccggt atcaggaagt ggtgtccggg tatctgttca accgcaaagc taacggcatc
      721 gatgaaatct ggtacatctg cccggaccgg gcgacgcagg tcagggtgca gcgggcaatc
      781 ctgtcggtgg atgaaatcgt gaacccacag accggcgagg cccgcaaaac ggcagagctg
      841 gatcgtgaac ggttgtttgc gtgctttaaa tttatgacaa cggagtgagg gattatggcg
      901 ctgtacatgg tatttattga agacgcggag gaccggaacg gcgagcgctg catcatgccg
      961 accagcgagc atctggaaga tatggtggtg ctcgcctggc agaccggggc gtatgttttc
     1021 tgttttgtcg accgggccca gcatctggcg atgatgaaat ccatcgggat gtatgacgag
     1081 cggctcaaca gcgacgaaaa agccacgtac tttttcactg gtaaaaatcc gcaatgggaa
     1141 cggaacaaac ccacaggaat gaagctgtgt gattacctct atcgcaaggt ggagcggttc
     1201 gccatcgaga acggtgtgca ggtggttgat gccgggaagt gctcaccgga gcagattaac
     1261 gcgatgattg acgtggtgag tgagcggcac ggggaaagcg cggtgaacga gaagctgaaa
     1321 gcggagctga tgcgggaata taacagccgc tattttaacg tgccggtacc tgaactgccg
     1381 gaggacaaac ctgcccgccg gtggtggccg ttcggcaaaa aataaatgcc agcaccggcc
     1441 atgccggtgc tgttattcct ccccgagctt atcccgcatc acctgccaga gtgtttccgc
     1501 atcctcgtgt agccgctcgc acatgtcggc cggggtatcc atatccagct cgttcagttt
     1561 atcgagcaat agcagcgact gctccttaat aaatttagcc atattgaggg ctgttttttc
     1621 ctgttttgtc atgattcttt ccttccgggc atggtgtgcg cctgtcaggt atactgtctc
     1681 aaaaatagcg gggtgagaca atggcgtggg tgacggtgag acaggcgacg gaactgacgg
     1741 gtaaagcccg aagttccctg tatcgggata tggcgaaagg ccgcgtatca taccgtacgg
     1801 aagctgatgg cggccgggtg gtggatacca gcgagcttat ccgggtgtac ggtgagttaa
     1861 gacaggatga gactcacgag cgggacgagt tgagactgtc gggtgagact gaaaaaatgg
     1921 cctccgacgc gttgatcgcg gagataaaag cactcaggga ggaggtggcc gggctacggc
     1981 aggaaatgca gtccatgaga ctgctggaac acaaaccgga gacagcagag accccggccg
     2041 gaacaccgcg ccggtggtgg ccgtggggaa aacgctgacg cgctgtgctt gtccaaccgg
     2101 cgacggccac gcaatgaatt gcgtccccgt caccggttat tagagaacaa tatggggatt
     2161 ctggcgctgg ccgaccacgg ccagaacccg gacttttctg gtctggtcat caacatcata
     2221 gaggacatgc tgtcccatca gactgatccg gcgaacgtcc tcgccatagc gtgagtccac
     2281 ttcatagagt cgcgggaagt cggccagttt gtccgccgca gcgagaaccc ggtcggtaac
     2341 gttctgcgca tcccacaggg atgaattttc cgcgatatag tcaaacagcg tcgttacatc
     2401 ctcgatggct ccctgcttcc attccacgtc atacggcatc gcgcagttcc tgataacggc
     2461 catgcaggcg ctggcggagt tgttccgtgg tcatacccgg acgcgtgtca gcacgggcgg
     2521 cggtcacttt ggcgcgaacc tgatcttcat actctgcctg ggccagttct tcgttagcca
     2581 gtcgctgcaa cagcgcagct ttgtccgggc caccttccgg cagtatgcgg ggcaggcttt
     2641 cagccagttc ccgaagaaag ttggcgtttt cgtagtgtgt agtgttgctc attgcggagc
     2701 ctcccgggtt aatgcggcaa tatgaaggaa gtttagcagg gggcggtctg aaagaatatg
     2761 gtcgtgtcag tcaggctgat gaagcagaaa gtcggtaaaa aagggcttac ggcatccatt
     2821 ttacgtcaaa acatatgcca tgcagttgcg tgcataggca tgcattagga taaaatttat
     2881 cgggcgcgtt tccggcagtt ttccgggggg gttgttgcct gttttatccc gtagccgccg
     2941 gaaacgccct gagcccgtct gagcggtgcg cgtaatgacg cgttatggta aatccactta
     3001 ctgtctgcct tcatagccat cgagataaac agctgaaatg gtgtcagcca gtagccttga
     3061 ttttttcggt gtgaggtctt tgctgcggta atcggtaccc gcaaattgtg ttgtgccttg
     3121 gagtgagttt aaaaattcct gactgtgaaa ctgctgaatg cgttgctgca catcactccg
     3181 tgattttccg gtcgctttat cctttgcgcc catatcccag aactgccgga aaaaaggcac
     3241 catcattcgt gttgtgtcag ctttctgggt ggaaaaattg ggatcaaaac cgtcatctga
     3301 agcatatacg taatgccggg tatgctgttc cacgctgccc tttggagcac aggcagtgag
     3361 agttgctgtc gctgttatca tggcgataag acacagtttg ctgccggaac gtctcatgag
     3421 cgtctcctta cctttaattc ttttttctca ttgagttata tatccgcagg ctatcttatc
     3481 atcagtttct gaccgtgttc agtgaccgaa caacccgaac cagacccccg tttcttttcc
     3541 tgtcccggag ggggctttct ggtatctcat ataatgattt cagtaaaaca tactgaggcc
     3601 ggagaacggc gccgtttgtg ctggtttttg ttgcatctgg aggggattgc gcatggtgtg
     3661 agtggctgtt tttttgatca ttttcacaaa aacgcaaatt tacagacttg acatgaaaaa
     3721 caaggcagcg taatttttat actgtatata aaaccagtgg ttatatgtac agtattttat
     3781 tttagtttat tgttttaaaa gtcaaagagg aaaagtatat gagtggcgga gacggacgag
     3841 gtccgggtaa ttcaggtctg ggacacaatg gtggtcaggc cagtgggaat gtgaacggta
     3901 catcaggtaa aggtggccct tcatcaggtg gtggtacgga tccaaacagc gggccgggct
     3961 ggggtacgac gcatacacct aacggggata ttcataacta taacccgggg gagtttggta
     4021 acggcgggag taaacccggt ggtaatggcg gtaacagcgg caatcatagc gggagctctg
     4081 gtggcggaca gtcttcggcc accgcgatgg ccttcggtct gcctgccctg gccactccgg
     4141 gggctgaagg actggcttta tccgtttccg gcgatgcgtt gtcggccgct gttgctgatg
     4201 tgctggctgc cctgaaaggt ccgtttaagt ttggtctgtg ggggattgcg atttacggag
     4261 tgctgccttc tgagatagca aaagatgatc cgaaaatgat gtcaaaaatc gtgacgtcat
     4321 tgccggcaga cacggtaaca gagacgccgg tcagctccct gccgctggac caggcgacgg
     4381 tcagcgtcac taaacgtgtg gcggatattg tgaaggacga gcggcagcat attgcggttg
     4441 tcaccggccg gccaatgagt gttcctgtgg tggatgcgaa accgacaaaa cgtccggggg
     4501 tattcagtgt gtcgattccg ggtctcccgt ctctgcaggt gagcgtacct aaaggtgttc
     4561 cggccgcgaa agccccgcca aaaggcatta ttgctgaaaa aggtgattca cgtccggctg
     4621 gttttacggc cggtggtaac tcccgtgagg ccgttattcg tttcccgaaa gagagcggac
     4681 agaaaccggt ctatgtgtcg gtgacggatg ttcttactcc ggcacaggta aaacagcgtc
     4741 tggaggaaga aaagcgtcgc cagcaggcat gggacgctgc tcacccggaa gaggggctga
     4801 aaagagagta tgataaagcg aaagctgagc tggatgctga agataaaaat attgcgacct
     4861 taaacagtcg cattgcatcg acagagaagg cgatccccgg tgcaagggct gctgttcagg
     4921 aagccgataa aaaggtaaaa gaggcagagg caaataaaga tgattttgtg acttataacc
     4981 cgcctcatga atatggctcc gggtggcagg atcaggttcg ctatcttgat aaggatattc
     5041 agaatcagaa tgagaaatta aaagctgccc agacatcttt aaacgaaatg aatgaatcct
     5101 tatccaggga taaggctgcg ctttccgggg cgatggagag ccggaaacaa aaggagaaaa
     5161 aagcgaagga tgcagaaaat aaattaaatg aggaaaagaa aaaacctcgt aagggaacta
     5221 aagattacgg ccatgattat tttcctgatc ccaagactga agatattaag gggttgggag
     5281 agttgaaaga gggtaaacct aaaaccccta aacaaggtgg tggtggtaag cgggcccgat
     5341 ggtatggtga taaaaagcgt aaaatttatg aatgggattc ccagcacggt gagcttgaag
     5401 ggtaccgcgc cagtgatggc gaacacctcg gggcattcga tccaaaaacg ggtaagcagg
     5461 ttaaagggcc ggatccaaaa cgaaacatta aaaaatatct ttaagaggta aatatggggc
     5521 ttaaattaca tattcattgg tttgataaga aaaccgaaga gtttaaaggt ggtgaatact
     5581 caaaagactt cggtgatgat ggttctgtca ttgaaagtct ggggatgcct ttaaaggata
     5641 atattaataa tggttggttt gatgttgaaa aatcatgggt ttcgatatta cagccacact
     5701 ttaaaaatgt aatcgatatt agtaaatttg attactttgt atcctttgtt taccgggatg
     5761 gtaactggta agcaaaagtt atcaaggatg atttgaaata cagacttccg gtgttcaggg
     5821 atgaatgccg gggtttttaa atacagggaa ggtatatgaa aaaggtaaag gttgtttttc
     5881 tttttatatt gattttttca ggctttcttc tggtcgcatg tcaggcaaac tatatccggg
     5941 atgttcaggg tggaacggtg gcaccatcgt cctcctctga actgacgggg atcgcggttc
     6001 agtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tcttttgccc
     6061 tgtaaacgaa aaaaccacct ggggaggtgg tttgatcgaa ggttaagtca gttggggaac
     6121 tgcttaaccg tggtaactgg ctttcgcaga gcacagcaac caaatctgtc cttccagtgt
     6181 agccggactt tggcgcacac ttcaagagca accgcgtgtt tagctaaaca aatcctctgc
     6241 gaactcccag ttaccaatgg ctgctgccag tggcgttttg cgtgcttttc cgggttggac
     6301 tcaagtgaac agttaccgga taaggcgcag cagtcgggct gaacgggggg ttcttgctta
     6361 cagcccagct tggagcgaac gacctacacc gagccgagat accagagtgt gagctatgag
     6421 aaagcgccac acttcccgca agggagaaag gcggaacagg tatccgggaa acggcagggt
     6481 tggaacagga gagcgcaaga gggagcgatt catcggaaac ggtgatgatc tttaagtcct
     6541 gtcgggtttc gccactcctg actgattcat ggttgagcca ccggctccca cagatgcacc
     6601 gaaaaagcgt ctgtttatgt gaactcagtc aggagggcgg agcctatgga aaaacgccac
     6661 ctgcgtggcc ttgccgtttt gcctcacatg ttagtcccct gcttattcac ggaatctgtg
     6721 ggtaactttg catgtgtccg cagcgcccgc cgcagtctca cggccggagc gtagcgaccg
     6781 agtgagcgag gaggcgcaat ttccccggca cctacaagac ggacaccgga cgcgctgatg
     6841 tggcatattg tcccctgatt aagtctttat attccctgat aataaattac caggataggt
     6901 gaatcatatc ggtttttcgc tgaacatttt acgttaacct atctctgttg cttttttctc
     6961 cttacggaga aatacaggtc atccccgcga gggcgtggct gacctcaggg ggatatcccc
     7021 tgcttacgct ggctggacgc caccgacgca cggtctctcc ccctctttct ggctccgtcc
     7081 ggctggacgc cggccacgcc agaaattcac ccggcccccg tcaggctcac gcctgaccac
     7141 ccacaaaaat ccacggtgct ggacgcacct ggcgaagcag caaagctgct cctgattttg
     7201 cgggtggtca gccgctcgct atggcgaact ctgacggggg ccggaacacc ggaaaagacc
     7261 gcactggggt gccgcctttt cctctcacac gctggggcgt gctcgttgtt cctccggggc
     7321 tgcgcctttt ccgcatgggg cggaaagagg ctggcctccg tggtgaaagc tgaacgcaat
     7381 caccacggac gtgaccgccg ccgccatgcc ttggggcatg tccgcggctt gtgcagcggg
     7441 ggctgcacat cacgccggca aaccatcgtc tgaaggagag agaacatgtt taatacggat
     7501 tcgcttgcct ggaagtggca cattccggag ctggcccggc agatttatcc gtgggtcagc
     7561 tggctgtacc aggagtatta cggtctggcc ggggctttcc tagttatcgg cctgtttttc
     7621 ggtctggctg aactttgggc cggacggatg cgttttgtgg ggctgagtca taaggagcga
     7681 cgggagtatg ccgatcgcta taagcgcgat aataaacggc cagcgccatt ttcggtgctg
     7741 gcctcgctgg attcgcccgg gcggatggtc agggcacgtc gacatgtctg gaactacgcg
     7801 tactggctga ccggcgcggc cggtctggcc cttctggggg ggctggttgt cgccgtgttt
     7861 atcgagagct ggggagaaac cgtcctgcgt ccggacctct acgcaaacgc agattattac
     7921 ctgctgcagc cggctaacct gttcgggatt gcctggtgcg cgggcgggct ggctgtcggg
     7981 attgtgttgg cgcggctgct ggcgttcctg ctgcttgacg gtcattttgc ggctgctgac
     8041 gaggcggtga acgcgaaact gaatcaggag agccggcgca gcagccagcg gacaggagag
     8101 atgaccgacg tgcgccacct gcatttcggg gagccggtac cggttaatgc gctggccgat
     8161 ttttcctcgg agcaggcccg gcaggagcag gcggtgtttc tgggtaagga tgagcagggc
     8221 aagccggtgc tggttccgcg tgatacctgg cgcaagacca acattcagat cctcggcctg
     8281 cccgggagcg gcaagagcgt gatggccacc aacgcgctca ttcgctgcgt acgcgatttc
     8341 ggggacgcgg tggtgtactt cgaccccaaa ggggacgcat gggcgccgca cgtcttccgg
     8401 gcgcactgtc cggacttcac cctgctggat ctgcggccgg ggaaaccggc gcagctgaat
     8461 ctgttccgcg atctcgacca gtacgcgctg aaaaacctgc tgattgccgg gcttaacctg
     8521 ggcgagaccg gcgacgtggc ggaccactac cgcatcagcg agcagaaagc ggcgaagctg
     8581 attgcggagc agtttccgca gggggcaaac attcagcagg tgctggcggc ggcgtacgcg
     8641 ctgccggagg ccctgaaaaa ggacgtgaag gggttgatca ctaggctgga aaacgtcgct
     8701 gacctgagcg tcctgcagac ggacagtggt atcgatgtgg cggggattat caacggcggc
     8761 ggctgcctgt acgttatcgg ctcgatggac gacgaggcgg tgatccgtgt gcaaaaaatg
     8821 ctgtttgccc gctgcgcgca aatcatcatt gcgcgggacg agttccggga gtggccacac
     8881 gtcagcgtga tgctcgatga aattaaatat ctgctgtcga agtatgtact gaacgcactg
     8941 gggacgctgc gcagccggga ctgcaatctg ttgctcgcgc accagtcgct gggcgatttc
     9001 gggcagtgcg ggcaggatct gcccgctgaa tttgtgaaaa ccacggtact ggataacacg
     9061 ccgatccgct ggttttaccg ggcggccagc ctggagtccg cgcagtgggc ggcggggcag
     9121 accggggaaa tcagggtgga cgtggagcgc cgccgtgcca gccgggaggc ggggaacgtg
     9181 gagcatatca gcggggacag cttcatccag aaggaggcgc ggccgctgtt tgacgtgaac
     9241 accctgcaac acctgccgga cgggttcgcg gtcatgaccg ggctgggtgt ggc
//
