LOCUS       NZ_CP036374            10060 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain WCHKP020037 plasmid p2_020037, complete
            sequence.
ACCESSION   NZ_CP036374
VERSION     NZ_CP036374.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07235711
            Assembly: GCF_002811305.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP020037
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 10060)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 22:35:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,792
            CDSs (total)                      :: 5,670
            Genes (coding)                    :: 5,545
            CDSs (with protein)               :: 5,545
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 43 of 125
            Pseudo Genes (incomplete)         :: 81 of 125
            Pseudo Genes (internal stop)      :: 16 of 125
            Pseudo Genes (multiple problems)  :: 15 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036374.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP020037"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_020037"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2017"
     gene            join(9821..10060,1..132)
                     /gene="tolA"
                     /locus_tag="CEA76_RS29520"
                     /old_locus_tag="CEA76_29515"
     CDS             join(9821..10060,1..132)
                     /gene="tolA"
                     /locus_tag="CEA76_RS29520"
                     /old_locus_tag="CEA76_29515"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            129..494
                     /locus_tag="CEA76_RS31435"
     CDS             129..494
                     /locus_tag="CEA76_RS31435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            669..947
                     /locus_tag="CEA76_RS29530"
                     /old_locus_tag="CEA76_29525"
     CDS             669..947
                     /locus_tag="CEA76_RS29530"
                     /old_locus_tag="CEA76_29525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            935..1249
                     /locus_tag="CEA76_RS29535"
                     /old_locus_tag="CEA76_29530"
     CDS             935..1249
                     /locus_tag="CEA76_RS29535"
                     /old_locus_tag="CEA76_29530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1413..1841
                     /locus_tag="CEA76_RS29540"
                     /old_locus_tag="CEA76_29535"
     CDS             1413..1841
                     /locus_tag="CEA76_RS29540"
                     /old_locus_tag="CEA76_29535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            1867..2046
                     /locus_tag="CEA76_RS29545"
                     /old_locus_tag="CEA76_29540"
     CDS             1867..2046
                     /locus_tag="CEA76_RS29545"
                     /old_locus_tag="CEA76_29540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2073..2603)
                     /locus_tag="CEA76_RS29550"
                     /old_locus_tag="CEA76_29545"
     CDS             complement(2073..2603)
                     /locus_tag="CEA76_RS29550"
                     /old_locus_tag="CEA76_29545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2610..3341)
                     /gene="mobC"
                     /locus_tag="CEA76_RS29555"
                     /old_locus_tag="CEA76_29550"
     CDS             complement(2610..3341)
                     /gene="mobC"
                     /locus_tag="CEA76_RS29555"
                     /old_locus_tag="CEA76_29550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(3341..5305)
                     /locus_tag="CEA76_RS29560"
                     /old_locus_tag="CEA76_29555"
     CDS             complement(3341..5305)
                     /locus_tag="CEA76_RS29560"
                     /old_locus_tag="CEA76_29555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6789..6938)
                     /locus_tag="CEA76_RS29570"
                     /old_locus_tag="CEA76_29565"
     CDS             complement(6789..6938)
                     /locus_tag="CEA76_RS29570"
                     /old_locus_tag="CEA76_29565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7023..7280)
                     /locus_tag="CEA76_RS29575"
                     /old_locus_tag="CEA76_29570"
     CDS             complement(7023..7280)
                     /locus_tag="CEA76_RS29575"
                     /old_locus_tag="CEA76_29570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(7290..8975)
                     /locus_tag="CEA76_RS29580"
                     /old_locus_tag="CEA76_29575"
     CDS             complement(7290..8975)
                     /locus_tag="CEA76_RS29580"
                     /old_locus_tag="CEA76_29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9302..9547
                     /locus_tag="CEA76_RS29585"
                     /old_locus_tag="CEA76_29580"
     CDS             9302..9547
                     /locus_tag="CEA76_RS29585"
                     /old_locus_tag="CEA76_29580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             2290..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6108..6686
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2614..3341)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 attgctgaag gaggagatcc gaaactctgt aaagcagcaa tatcagccgt tacccacgcc
       61 aaaattccgc cggcaccgga tgaaaatacc tggcaaatat tcaaaaacgc ccctctggat
      121 ttcagaccat gaaagctttt actctgacag tgctggcggc catgttcctc actccggtac
      181 atgcgatacc actaaaaccc gtaaccacac tgcagccaac ctacccggta caggccgcag
      241 caaaacgcat cgaaggtcag gtgaccgcca cgtttgatat tctggacgat ggacgagtgg
      301 acaacgtgtc gcttaccggc tgtcaccttt tttttcggga aacgctgacg gcaatgaggc
      361 actggctata tcccgcaggg cacccggcaa tgcgacaggt tataaatttt gaattcagtc
      421 tgaaaaatga cggcttacag gatccgcgac cagtcaccac ccaaaagctc aatgatgatg
      481 gttgccgcca gtaacaggtc ctttaacccg caggctgcaa tagtcctgga tgatgagtga
      541 gcctgtagca gccctgatta gtggaagcga agctgaaaaa tgggcagcaa aagccgtatt
      601 tctggcaaat agcgccgcca cggattaaag tagtcatgtg taatacatat gactacaaag
      661 gggcttttat gtcatcagca acatccatca ggctcgatga agaacttaag gaccggctga
      721 aaacgctggc cgatgaccgg caccgctcag cgcacgcgct aatgctggag gcaatcaccg
      781 aatacatcga ccgggaagag aagcgcagcc agtacctgcg ggacggacag gccgcatggc
      841 agcactatca ggaaacgggt ctgcacctta ctgcggaaga agcggaagcc tggataagta
      901 cctggggaac tgagaacgag caggatgctc cgccatgcca caggtaacga tttccgcgct
      961 ggcacagcgc gatttacaac gccttcagga ctttctcaaa accaaaaatc ggctggcagc
     1021 cagaaaagga ggtgaggtga tcgtccgggc tatccagcaa ctgaaaacat tgccagacat
     1081 tggccgcccg gtgccgtttc tgccgctgga atataaggaa ctggtgatcg ggtttggcga
     1141 cagtggctat gtgatgctct accgccacga ccgggaaatg gaccagattg tgatcgtcac
     1201 cgtcaggcat cagaaagaat ccgggtatcc gggggcagac agcctctaat aaccgatgac
     1261 gggggcgcaa ttcattgcgt ggccgtcgcc ggtttgacaa gcgcagcgcg tcagaaaacc
     1321 acatcctgaa cggaaaaacg ggtaaaattc agcgatacat ttaccgtgag aaattacaca
     1381 cctttcacac ccgataaaat caggggtata cagtggcgaa aacattgctc aacctttcac
     1441 aggcagcaca agccgcgggc attaccagaa gaacgctata caatcatgta aaacagggaa
     1501 aggttaccgt gtcacgtgac gggaaaaaca accctgtcgt ggatgtgtca gaactgatcc
     1561 gtgtatacgg aaatgtgaat atccctgaga aacaaattcc cggcatttca caccgtgaaa
     1621 atacacaaaa aaatttccca caggaacagc ttctggcaat gcaaaaggaa ctggccgatc
     1681 tccggcaggc tgtcaccctg atgctggaag ataaaacatc ccgggaagaa gaacgccgcc
     1741 agcacgatga tgagcgccgg aaactccagg ccgaagtgga cagattgaca acagaactga
     1801 cacagaagaa aaaacgcttc tggtccggct ggtttagctg agaacttacc gaagggaatg
     1861 gagaacatga cgaagcagga aaaaactgcc ctcaatatgg ctaaattcat taaggatcag
     1921 tcgctgctgt tgctggaaaa gctgaacgag ctggacatgg ataccccggc cgacatgtgc
     1981 gaacggcttc acgaggatgc ggaaacactc tggcaggtga tgcgggataa gctcggggaa
     2041 gaataacagc accggccatg ccggtgctgg cgttattttt tgccgaacgg ccaccaccgg
     2101 cgggcgggtt tgtcctccgg cagttcaggc accggcacgt taaaatagcg gctgttatat
     2161 tcccgcatca gctccgcttt cagcttctcg ttcaccgcgc tttccccgtg ccgctcgctg
     2221 accacctcaa tcatcgcgtt aatctgctcc ggtgagcact tcccggcatc aaccacctgc
     2281 acaccgttct cgatggcgaa ccgctccacc tttcgataga ggtaatcaca cagcttcatt
     2341 cctgtgggtt tgtcccgttc ccattgcgga ttttcaccgg tgaaaaagta cgtggctttt
     2401 tcgtcgctgt tgagccgctc gtcatacatc ccgatggatt tcatcatcgc cagatgctgg
     2461 gcccggtcga caaaacagaa aacatacgcc ccggtctgcc aggcgagcac caccatgtct
     2521 tccagatgct cgctggttgg cataatgcag cgctcgccgt ctttgtcttc cacgtcctca
     2581 ataaatacca tgtacagcgc cataatccct cactccgttg tcataaattt aaagcacgcg
     2641 aacagcctct cccggtccag ctccaccgtt ttgcgggcct cgccggtctg tggattcacg
     2701 atttcatcca ccgccagaat cgcccgctgt acccggacaa gcgtcggccg gtcagggcag
     2761 atgtaccaga tttcatcgat gccgttcgcc cggcggttga acagataccc ggacaccact
     2821 tcctgatacc gcctgcggct cttgacggtg cgctctacct caaatgccac cgttctgccg
     2881 tccagctccg ccagcgcgtc cggcactttc atccctttca gggccatacg gtgcaggtac
     2941 cgccagccgg tggccccccg gccctccatg gagaggcgca gggactgcac ggccagccgg
     3001 tgcggcagct gcgccgccga cacccttgac ggctgaaacg gaataacgtc cgtcagcggc
     3061 tcgtcctcgt cgaacgagaa cgccaccccg tgcggcgtca gcccccacac gctgacctga
     3121 cggccgtcaa ccgggtattt cgcccggacc agcagccctt tcgccaccag actgtccagg
     3181 aagcggtaaa ccggctgtac cgccgcaatc cccagccgtc gcgcaaccag ctccgccgtg
     3241 gaacacagct cttcccgcag ccagcgcagg gtcagccgtg ttttctccgc gttccgcgcc
     3301 tgccgcttcg cgtggctgac gttgtactgc tccagtgtca tcagtaaagc cccgcaaaat
     3361 catcatccgc ttttggtccg ccgccgtcgg ccggaggcag gtactccgcc agcgggtccg
     3421 ttttcgccag caccgggaag gttttcagcg aaatttcccg gcgctcaacc cgcagcgggc
     3481 tgctgaacgc cagccgcgcc acgcccagcc cggtcatcac cgcgaacccg tcaggcaggt
     3541 gttgcagggt gttcacgtca aacagcggcc gcgcctcctt ctggatgaag ctgtcgccgc
     3601 tgatgtgctc cacgttcccc gcctcccggc tggcccggcg gcgctccacg tccacccgga
     3661 tctccccggt ctggcccgcc gcccactgcg cggactcctg gctggccgcg cgatagaacc
     3721 agcggatcgg cgtgttatcc agtaccgtgg ttttcacaaa atcagcgggc agatcctgcc
     3781 cgcactgacc gaagtcgccc agcgactggt gcgcgagcag caggttgcag tcccggctgc
     3841 gcagcgtccc cagcgcgttc agcacgtact tcgacagcag atatttaatc tcatcgagca
     3901 tgatgctcgc gtgcggccac ctccggaact catcccgcgc aatgatgatt tgcgcgcagc
     3961 gggcaaacag catcttctgc acccggatca ccgcctcatc gtccatcgag ccgatgacgt
     4021 acaggcagcc gccgccgttg attatccccg ccacatcgat accgctgtcc gtctgcagga
     4081 cgctcaggtc ggccacgttc tccagcttgg tgatcagccc cttcacgtcc tttttcaggg
     4141 cctccggcaa cgcgtacgcc gccgccagca cctgctgaat gtttgccccc tgcgggaact
     4201 gctccgcaat cagcttcgcc gctttctgct cgctgatgcg gtagtggtcc gccacgtcgc
     4261 cggtctcact caggttaaac ccggcaacca gcagattctt cagggcgtac tggtcgagat
     4321 cgcggaacag attcagctgc gccggtttcc ccggccgcag atccagcagg gtgaagtccg
     4381 ggcagtgcgc ccggaagacg tgcggcgccc acgcgtcccc tttggggtcg aagtacacca
     4441 cagcgtcccc gaaatcgcgt acgcagcgaa tgagcgcgtt ggtggccatc gcgctcttgc
     4501 cgctcccggg caggccgagg atctgaatat tggtcttgcg ccaggtatca cgagggacca
     4561 gcaccggctg gccctgctca tccttaccca gaaacaccgc ctgctgtttc cgggcctgct
     4621 ccgtggaaaa atcggccagc gcgttaaccg gtaccggctc cccgaaatgc aggtggcgga
     4681 cgtctgtcat ttctccggtc cgctggctgc tgcgccggct ctcctgattc agtttcgcgt
     4741 tcaccgcctc gtcagcggcc gcaaaatgac catcaagcag caggaacgcc agcagccgcg
     4801 ccagaaggac gccggcgaca agccctccgg cacaccaggc tatcccgaac aggttagccg
     4861 gctgcagcag gtaataatct gcgttcgcat agaggtccgg gcgcaggacg gtttctcccc
     4921 agctctcgat aaacacggcg acaaccagcc cccccagaag ggccagaccg gcagcgccgg
     4981 tcagccagta cgcgtagttc cagacatgtc gccgtgccct gaccatccgc ccgggtgaat
     5041 ccagcgaggc cagcaccgaa aatggcacag tccggttatt atcgcgctta tagcgatcgg
     5101 catactcccg ccgcgcctta tggctcagcc ccacaaaacg cattcggccg gcccagagtt
     5161 cagccagacc gaaaaacagg ccgataacca gaaaaaaacc ggccagactg taatactccc
     5221 ggtacagcca gctgacccac gggtaaatct gccgggccag ctccgggata tgccactgcc
     5281 aggcaagcga atccgtatta aacatgcaat ctctctcctt cagacgacgg gtttaccggc
     5341 gtgatgcgca gcccccgctg cacaagccgc ggacatgccc caaggcatgg cggcggcggt
     5401 cacgtccgtg gtgattgcgt tcagctttca ccgcggaggc cagcctcttt ccgccccatg
     5461 cggaaaaggc gcagccccgg aggaacaacg agcacgcccc agcgtgtgag aggaaaaggc
     5521 ggcaccccag tgcggtcttt tccggtgttc cggcccccgt cagagttcgc catagcgagc
     5581 ggctgaccac ccgcaaaatc aggagcagct ttgctgcttc gtcaggtgcg tccagcaccg
     5641 tggatttttg tgggtggtca ggcgtgagcc tgacgggggc cgggtgaatt tctggcgtgg
     5701 ccggcgtcca gccggacaga gccagaaaga ggggaaaaga ccgtgcgtcg gtggcgtcca
     5761 gccagcgtaa gcaaggtctt ttcccctgaa gtcagccacg ccccagcggg gatgacttgc
     5821 atttctccgc aaggagaaaa aagcaacaga gataggttaa cgtaaaatgt tcagcgaaaa
     5881 accgacatgg ttcacctatc ctgataattg atcgtcaggg aatagaaaga cttaatcagg
     5941 ggacaatatc ccacatcagc gcgtccggtg accgtcttgc aggtgccggg gaaattgcgc
     6001 ctcctcgctc actcggtcgc tacgctccgg gcgtgagact gcggcgggcg ctgcggacac
     6061 atacaaagtt acccacagat tccgtggata agcaggggac taacatgtga ggcaaaacgg
     6121 cagggccgcg ccggtggcgt ttttccatag gctccgccct cctgccagag ttcacataaa
     6181 cagacgcttt ttcggtgcat ctgtgggagc cgtgaggctc aaccatgaat ctgacagtac
     6241 gggcgaaacc cgacaggact taaagatccc caccgtttcc ggtaggtcgc tccctcttgc
     6301 gctctcctgt tccgaccctg ccgtttaccg gatacctgtc cgccgttctc ccttgcggga
     6361 agtgtggcgc tttctcatag ctcacacact ggtatctcgg ctcggtgtag gtcgttcgct
     6421 ccaagctggg ctgtaagcaa gaactccccg ttcagcccga ctgctgcgcc ttatccggta
     6481 actgttcact tgagtccaac ccgggaaagc acgcaaaacg ccactggcag cagccattgg
     6541 taactgggag ttcgcagagg atttgtttag ctaaacacgc ggttgctctt gaagtgtgcg
     6601 ccaaagtccg gctacactgg aaggacagat ttggttgctg tgctctgcga aagccagtta
     6661 ccacggttaa gcagttcccc aactgactta accttcgatc aaaccacctc cccaggtggt
     6721 tttttcgttt acagggcaaa agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6781 gatcttttct actgaaccgc gatccccgtc agttcagagg aggacgatgg tgccaccgtt
     6841 ccgccctgaa catcccggat ataatttgcc tgacatgcgg ccagaagaaa gccagcaaca
     6901 atcaatataa aaagaaaaat agtctttacc tttttcatat actttccctg tatttaaaga
     6961 ctccggcatt catccctgaa caccggaagt cagtatttca aatcatcctt gataattttt
     7021 tgttaccagt taccgtcccg ataatcaaaa gctatataac aattatccac atcaggtttt
     7081 atttgatttt taaaaaatgg ctgtagtaaa ggaacccatt ctttcttcaa atcaaactca
     7141 ccattattaa tattatcctt taaaggcatt cccaagcttt caatgacaga accatcgtca
     7201 ccaaagtctt tagaatactc ttccccttta aactcttcag ttttcttatc aaaccatgtc
     7261 aaacgtaatt taagccccat atttacctct caaagatatt ttttaatgtt ccgttttgga
     7321 tctggccctt taacctgctt acccgttttg ggatcaaatg cgcctaggtg ttcgccatca
     7381 ctggcacgat acccttcaag ctcaccgtgc tgggaatccc attcataaat tttacgtttt
     7441 ttatcgccat accatcgggc ccgcttacca ccaccacctt gtttaggtgt tttaggatca
     7501 cctcgcttta actctccaag tccctttatg tcttctgttt taggtactgg atgataatca
     7561 tggccgtagt ctttagtccc cttgcgaggc ttgttctttt cctcatttaa cttattttct
     7621 gcatccttcg cttttttctc cttttgtttc cggctctcca tcgccccggt aagtgcagcc
     7681 ttatctctgg ataaggattc attcattgcg tttaaagatg cctgagccgc ttttaatttc
     7741 tcattctgat tctgaatatc cttatcaaga tagcgaacct gatcctgcca cccggagcca
     7801 tattcatgag gcgggttata agtcacaaaa tcatctttat ttgcctctgc ctcttttacc
     7861 tttttatcgg cttcctgaac agcagccctt gcaccgggga gcgccttctc tgtcgatgca
     7921 atgcggctgt ttaaggtcgc aatattttta tcttcggcat ccagctcagc tttcgcttta
     7981 tcatagtctc ttttcagccc ctcttccgga tgagcggcgt cccatgcctg ctggcgacgc
     8041 ttttcttcct cctgacgctg ttttacctgt gccggggtaa gaacatctgt caccgacaca
     8101 taaaccggct tctgtccggt ctctttcggg aaacgaataa cggcctcacg ggagttacca
     8161 ccggctgtaa aaccagccgg acgtgaatca cctttttcag caacaatgcc ttttggcggg
     8221 gctttcgctg tcggaacacc tttcggtacg ctcacctgca gagacgggag acccggaatc
     8281 gacacactga atacccccgg acgttttgtc ggtttcgcat ccaccacagg gacactcatt
     8341 ggccgacctg cgacaaccgc aatatgctgc cgctcatcct tcaccacatc cacaacccgt
     8401 tgtctgacac gaaccgtcgc ctggtccagt ggtaaagtac ttgccggagt ctccgtcacc
     8461 gcatcggccg gtaatgacgt cataattttt gacatcattt tcggatcatc ttttgcaatc
     8521 tcagaaggca gcacaccgta gatcgcaatc ccccacagac caaacttaaa cggccccttc
     8581 agggcagcca gcacatcagc aacggcggcc gacaacgcat cgccggaaac tgataaagcc
     8641 agtccttcag cccccggagt agccagggca ggcagaccga aggccatcgc agtggccgaa
     8701 gactgtccgc caccagagct accgctatga ttgccgctgt taccaccatt accaccgggt
     8761 ttactcccgc cgttaccaaa ctcccccgga ttatagttat gaatatctcc attcggcgta
     8821 tgcgtcgtac cccagcccgg cccgctgttt ggatccgtac caccacctga tgaagggcca
     8881 cctttaccag acgtaccgtt cacattccca ctggcctgac caccattatg tcccagacct
     8941 gaattacccg gacctcgacc gtctccacca ctcataccgt tttcctcttt gacatttaaa
     9001 acaataaatt aaagccaaca tactgtacat atacacagta taattattac cttactcctc
     9061 ccgttttcag gtgtcaatat atgagattag ttctcatctt caggcacaaa aaagcgcccg
     9121 gagccacctg taagcagatc gcgtcttttc ccgaaaattt ctcatggcaa caaattttat
     9181 tatttaagac attcatcaca cttacatttc agatggtata gacacatctg aaagctaact
     9241 ggcatgagga gaggacctgc ggttaaactg ttcgggaaac ctgagacgcc cggaggccac
     9301 tatgacgcac ctgaaactga ctcccgctga agaaaagcgc ctggcggacg cctttgtcgc
     9361 tcaggcactg gccatgggcc tgaaagggaa caaaacgctg ccaaaggcgg taaaacaaca
     9421 ggtgcgggaa agggtgcttc gtgccattca ggacgaacgt gaagccaggc gggcgcagga
     9481 aatgcgtcgg gagaaatacg aggctacgga aaccaccttc acctggcagc cacagcgccg
     9541 ccggtaaggg gtatttacca taacgtcgca ttgcccgcac cgctcagacg gtctgagggc
     9601 gtttccggcg gctacgggat aaaacaggca acaaaccacc cggaaaaccg ccggaaacgc
     9661 gcccggtaaa ttgtaacccg ctgcatgcct atgcacgcaa ctgcatggcg ggccgtttgg
     9721 tgtaaaaacg atacgataag ccgtgtttat gtaaatgaac gtggccgtaa agagtgacaa
     9781 atatacatgt aacatttttt actgacaggt catcaacccg atgcgtttaa taccctttat
     9841 cctgctggca ttgtgcagcc ccttactcct gacggcctgt aagcattcca ccacccagcc
     9901 gccgaaaatt tatactctgg ataagtcatc taaaatcagt agctacgctg cggccatcaa
     9961 gaccgctgtt gaaatgcaga tgtttgaacc agaggcctat gcgggaaaaa tgtgcaccgt
    10021 gcggatatcc atacaacgtg atggcactct gaacagcgcc
//
