LOCUS       NZ_CP036369            12746 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain WCHKP115068 plasmid p2_115068, complete
            sequence.
ACCESSION   NZ_CP036369
VERSION     NZ_CP036369.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09688180
            Assembly: GCF_004322795.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12746)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP115068
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12746)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-FEB-2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 22:29:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,647
            CDSs (total)                      :: 5,526
            Genes (coding)                    :: 5,385
            CDSs (with protein)               :: 5,385
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 43 of 141
            Pseudo Genes (incomplete)         :: 95 of 141
            Pseudo Genes (internal stop)      :: 15 of 141
            Pseudo Genes (multiple problems)  :: 12 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036369.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12746
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP115068"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_115068"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2018"
     gene            complement(<2..1150)
                     /locus_tag="DVJ72_RS28745"
                     /old_locus_tag="DVJ72_28750"
                     /pseudo=""
     CDS             complement(<2..1150)
                     /locus_tag="DVJ72_RS28745"
                     /old_locus_tag="DVJ72_28750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012966.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(1255..2742)
                     /gene="ltrA"
                     /locus_tag="DVJ72_RS28750"
                     /old_locus_tag="DVJ72_28755"
     CDS             complement(1255..2742)
                     /gene="ltrA"
                     /locus_tag="DVJ72_RS28750"
                     /old_locus_tag="DVJ72_28755"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            3387..3632
                     /locus_tag="DVJ72_RS28760"
                     /old_locus_tag="DVJ72_28765"
     CDS             3387..3632
                     /locus_tag="DVJ72_RS28760"
                     /old_locus_tag="DVJ72_28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3906..4277
                     /gene="tolA"
                     /locus_tag="DVJ72_RS28765"
                     /old_locus_tag="DVJ72_28770"
     CDS             3906..4277
                     /gene="tolA"
                     /locus_tag="DVJ72_RS28765"
                     /old_locus_tag="DVJ72_28770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            4274..4639
                     /locus_tag="DVJ72_RS30595"
     CDS             4274..4639
                     /locus_tag="DVJ72_RS30595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            4814..5092
                     /locus_tag="DVJ72_RS28775"
                     /old_locus_tag="DVJ72_28780"
     CDS             4814..5092
                     /locus_tag="DVJ72_RS28775"
                     /old_locus_tag="DVJ72_28780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            5080..5394
                     /locus_tag="DVJ72_RS28780"
                     /old_locus_tag="DVJ72_28785"
     CDS             5080..5394
                     /locus_tag="DVJ72_RS28780"
                     /old_locus_tag="DVJ72_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5558..5986
                     /locus_tag="DVJ72_RS28785"
                     /old_locus_tag="DVJ72_28790"
     CDS             5558..5986
                     /locus_tag="DVJ72_RS28785"
                     /old_locus_tag="DVJ72_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            6012..6191
                     /locus_tag="DVJ72_RS28790"
                     /old_locus_tag="DVJ72_28795"
     CDS             6012..6191
                     /locus_tag="DVJ72_RS28790"
                     /old_locus_tag="DVJ72_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6218..6748)
                     /locus_tag="DVJ72_RS28795"
                     /old_locus_tag="DVJ72_28800"
     CDS             complement(6218..6748)
                     /locus_tag="DVJ72_RS28795"
                     /old_locus_tag="DVJ72_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6755..7486)
                     /gene="mobC"
                     /locus_tag="DVJ72_RS28800"
                     /old_locus_tag="DVJ72_28805"
     CDS             complement(6755..7486)
                     /gene="mobC"
                     /locus_tag="DVJ72_RS28800"
                     /old_locus_tag="DVJ72_28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(7486..9450)
                     /locus_tag="DVJ72_RS28805"
                     /old_locus_tag="DVJ72_28810"
     CDS             complement(7486..9450)
                     /locus_tag="DVJ72_RS28805"
                     /old_locus_tag="DVJ72_28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(10934..11083)
                     /locus_tag="DVJ72_RS28815"
                     /old_locus_tag="DVJ72_28820"
     CDS             complement(10934..11083)
                     /locus_tag="DVJ72_RS28815"
                     /old_locus_tag="DVJ72_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(11168..11425)
                     /locus_tag="DVJ72_RS28820"
                     /old_locus_tag="DVJ72_28825"
     CDS             complement(11168..11425)
                     /locus_tag="DVJ72_RS28820"
                     /old_locus_tag="DVJ72_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(11435..>11977)
                     /locus_tag="DVJ72_RS28825"
                     /old_locus_tag="DVJ72_28830"
                     /pseudo=""
     CDS             complement(11435..>11977)
                     /locus_tag="DVJ72_RS28825"
                     /old_locus_tag="DVJ72_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            complement(11994..12691)
                     /locus_tag="DVJ72_RS28830"
                     /old_locus_tag="DVJ72_28835"
     CDS             complement(join(11994..12442,12442..12691))
                     /locus_tag="DVJ72_RS28830"
                     /old_locus_tag="DVJ72_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223174630.1"
                     /translation="MASVSVCCPSCSATEGVMRNGKSTAGHQRYLCSHCRKTWQLTFTY
                     AASQPGTHQKIIDMAMNGVGCRATARLMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRAVVAHVFGERTMATLERLLSLLSVFDVVVW
                     MTDGWPMYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     BGC             1..6150
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          10253..10831
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6759..7486)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 caaaatcatc tttatttgcc tctgcctctt ttaccttttt atcggcttcc tgaacagcag
       61 cccttgcacc ggggagcgcc ttctctgtcg atgcaatgcg gctgtttaag gtcgcaatat
      121 ttttatcttc ggcatccagc tcagctttcg ctttatcata gtctcttttc agcccctctt
      181 ccggatgagc ggcgtcccat gcctgctggc gacgcttttc ttcctcctga cgctgtttta
      241 cctgtgccgg ggtaagaaca tctgtcaccg acacataaac cggcttctgt ccggtctctt
      301 tcgggaaacg aataacggcc tcacgggagt taccaccggc tgtaaaacca gccggacgtg
      361 aatcaccttt ttcagcaaca atgccttttg gcggggcttt cgctgtcgga acacctttcg
      421 gtacgctcac ctgcagagac gggagacccg gaatcgacac actgaatacc cccggacgtt
      481 ttgtcggttt cgcatccacc acagggacac tcattggccg acctgcgaca accgcaatat
      541 gctgccgctc atccttcacc acatccacaa cccgttgtct gacacgaacc gtcgcctggt
      601 ccagtggtaa agtacttgcc ggagtctccg tcaccgcatc ggccggtaat gacgtcataa
      661 tttttgacat cattttcgga tcatcttttg caatctcaga aggcagcaca ccgtagatcg
      721 caatccccca cagaccaaac ttaaacggcc ccttcagggc agccagcaca tcagcaacgg
      781 cggccgacaa cgcatcgccg gaaactgata aagccagtcc ttcagccccc ggagtagcca
      841 gggcaggcag accgaaggcc atcgcagtgg ccgaagactg tccgccacca gagctaccgc
      901 tatgattgcc gctgttacca ccattaccac cgggtttact cccgccgtta ccaaactccc
      961 ccggattata gttatgaata tctccattcg gcgtatgcgt cgtaccccag cccggcccgc
     1021 tgtttggatc cgtaccacca cctgatgaag ggccaccttt accagacgta ccgttcacat
     1081 tcccactggc ctgaccacca ttatgtccca gacctgaatt acccggacct cgaccgtctc
     1141 caccactcat accgttttcc tctttgacat ttaaaacaat aaattaaagc caacatgtcg
     1201 cggtagggat acccgttacc ggataccccc cgcacagatc ccggcgtgcg cgatttacgc
     1261 accgggctcc tgcctcgggt gtctggcggt gaaccgctcc acaggccatg gatgaagaac
     1321 ccgaaccctt ggtagccatg cggctgccag tttgtttgct ttcgtccagg tcgtatcatc
     1381 cttctggctc ctgcgcctga gcgcccggcg ccagaggttt gttacgtgtg tcctgaactt
     1441 ctgcatggtg gggaagttgc ccggtaccga gtgatagttc aggtatccct gaaccactct
     1501 cctgagccat tttccctgtt cggggattga gtaatgccag cgccttcgca gaccgtcttt
     1561 gatggctttc agagttgccg tcatccgatc ccggcgggtc tttcgtatca gcatgaacct
     1621 gccgttgcga tctttcccgc tgatgtgcgt gaacccgagg aagttgaacg tttctggttt
     1681 gccttttccc ctgatggcac ggttttcggc agcgaagcgg ccgaactcca tcagacgggt
     1741 tttctccggg tgaaccgtga gtccgaactc cctcagtctg cgctgcatgg ctatacggaa
     1801 gcgccgggca tcgtatcgtt tgtcgaaccc gatgacgatg tcatcggcgt atctgaccat
     1861 taccacattg cctgtggcat agcgacgtcg ccactgatgc gcccacagat cgaagacgta
     1921 gtggaggtat atgtttgcca gcagcggtga gatgaccgca ccctgtgggg tgccttcctc
     1981 cgttgctcgc cattgaccct cctccgacgt cccggctgtg agccacttac gtatgagcct
     2041 gattaccctc cggtcgccga tccgatgctc tgtgaacctg atcagccatt cgtggctcac
     2101 cctgtcgaag aactgactga tgtcggcatc cagtacccag tttacgttag tgcgtaccag
     2161 ccctgtggcc agtgcgtcca gtgcatcgtg ctggcttcgc ccgggtctga acccgtatga
     2221 gaaccccata aagtcgtttt catagactgc gttcaggatt ttcaccagcg catactggac
     2281 gatcttgtcc tccagcgagg cgatgccgag cgggcgttgt tttccatccg cttttgggat
     2341 gtagtgacgc ctgccgggct gcgccctgta gctgccctga tgtagcctcc ggtgcagatc
     2401 tgttatgttg ttcttcatgt ttccggcgta gtccatccac ctgatgccat ccactccggc
     2461 ggccgctttc ctgctcaggg agaggaatgc ggcttccagt gcttcgactg tcagcaggtg
     2521 gaacaatgct gtaaaccgtt ctttcttccg ctgcttcgca gcttcccgca cgcgtgacag
     2581 cctctgtgac atgctttccc ggctctgtgt ccggcgcatg tgtggctgtt ccgcgttccc
     2641 cttggccccg ctccttcgct ccactgactc cgctcctttc gggttgttcg cctgcttcgc
     2701 cgctactatg agcgagtccg acttctcctc tccgtacatc accggctatg actcctcgtc
     2761 ttcccggtgc gggccatctc cgacactggc agatggtcag aggggagatc tcccggttcc
     2821 cgcgtagaga tcgtattgac atgccagggt ctcagacccc gccgggtcca tgtggcactc
     2881 gcagtatcgc accctatgat gttgccttcc gttaacagta caacgtcggc acccggtaat
     2941 ttaatataca tttcgtggct caatggctgg cctgtcaaca cccctgtcaa cgcttcgccc
     3001 catacctcgc ggtatgcaac gcatgactcg gggaccttgt ggattgctgg tccttcaatg
     3061 gtcggggact ttcacccctt gatctctaac cggtctcccg gcgcacactg tacatataca
     3121 cagtataatt attaccttac tcctcccgtt ttcaggtgtc aatatatgag attagttctc
     3181 atcttcaggc acaaaaaagc gcccggagcc acctgtaagc agatcgcgtc ttttcccgaa
     3241 aatttctcat ggcaacaaat tttattattt aagacattca tcacacttac atttcagatg
     3301 gtatagacac atctgaaagc taactggcat gaggagagga cctgcggtta aactgttcgg
     3361 gaaacctgag acgcccggag gccactatga cgcacctgaa actgactccc gctgaagaaa
     3421 agcgcctggc ggacgccttt gtcgctcagg cactggccat gggcctgaaa gggaacaaaa
     3481 cgctgccaaa ggcggtaaaa caacaggtgc gggaaagggt gcttcgtgcc attcaggacg
     3541 aacgtgaagc caggcgggcg caggaaatgc gtcgggagaa atacgaggct acggaaacca
     3601 ccttcacctg gcagccacag cgccgccggt aaggggtatt taccataacg tcgcattgcc
     3661 cgcaccgctc agacggtctg agggcgtttc cggcggctac gggataaaac aggcaacaaa
     3721 ccacccggaa aaccgccgga aacgcgcccg gtaaattgta acccgctgca tgcctatgca
     3781 cgcaactgca tggcgggccg tttggtgtaa aaacgatacg ataagccgtg tttatgtaaa
     3841 tgaacgtggc cgtaaagagt gacaaatata catgtaacat tttttactga caggtcatca
     3901 acccgatgcg tttaataccc tttatcctgc tggcattgtg cagcccctta ctcctgacgg
     3961 cctgtaagca ttccaccacc cagccgccga aaatttatac tctggataag tcatctaaaa
     4021 tcagtagcta cgctgcggcc atcaagaccg ctgttgaaat gcagatgttt gaaccagagg
     4081 cctatgcggg aaaaatgtgc accgtgcgga tatccataca acgtgatggc actctgaaca
     4141 gcgccattgc tgaaggagga gatccgaaac tctgtaaagc agcaatatca gccgttaccc
     4201 acgccaaaat tccgccggca ccggatgaaa atacctggca aatattcaaa aacgcccctc
     4261 tggatttcag accatgaaag cttttactct gacagtgctg gcggccatgt tcctcactcc
     4321 ggtacatgcg ataccactaa aacccgtaac cacactgcag ccaacctacc cggtacaggc
     4381 cgcagcaaaa cgcatcgaag gtcaggtgac cgccacgttt gatattctgg acgatggacg
     4441 agtggacaac gtgtcgctta ccggctgtca cctttttttt cgggaaacgc tgacggcaat
     4501 gaggcactgg ctatatcccg cagggcaccc ggcaatgcga caggttataa attttgaatt
     4561 cagtctgaaa aatgacggct tacaggatcc gcgaccagtc accacccaaa agctcaatga
     4621 tgatggttgc cgccagtaac aggtccttta acccgcaggc tgcaatagtc ctggatgatg
     4681 agtgagcctg tagcagccct gattagtgga agcgaagctg aaaaatgggc agcaaaagcc
     4741 gtatttctgg caaatagcgc cgccacggat taaagtagtc atgtgtaata catatgacta
     4801 caaaggggct tttatgtcat cagcaacatc catcaggctc gatgaagaac ttaaggaccg
     4861 gctgaaaacg ctggccgatg accggcaccg ctcagcgcac gcgctaatgc tggaggcaat
     4921 caccgaatac atcgaccggg aagagaagcg cagccagtac ctgcgggacg gacaggccgc
     4981 atggcagcac tatcaggaaa cgggtctgca ccttactgcg gaagaagcgg aagcctggat
     5041 aagtacctgg ggaactgaga acgagcagga tgctccgcca tgccacaggt aacgatttcc
     5101 gcgctggcac agcgcgattt acaacgcctt caggactttc tcaaaaccaa aaatcggctg
     5161 gcagccagaa aaggaggtga ggtgatcgtc cgggctatcc agcaactgaa aacattgcca
     5221 gacattggcc gcccggtgcc gtttctgccg ctggaatata aggaactggt gatcgggttt
     5281 ggcgacagtg gctatgtgat gctctaccgc cacgaccggg aaatggacca gattgtgatc
     5341 gtcaccgtca ggcatcagaa agaatccggg tatccggggg cagacagcct ctaataaccg
     5401 atgacggggg cgcaattcat tgcgtggccg tcgccggttt gacaagcgca gcgcgtcaga
     5461 aaaccacatc ctgaacggaa aaacgggtaa aattcagcga tacatttacc gtgagaaatt
     5521 acacaccttt cacacccgat aaaatcaggg gtatacagtg gcgaaaacat tgctcaacct
     5581 ttcacaggca gcacaagccg cgggcattac cagaagaacg ctatacaatc atgtaaaaca
     5641 gggaaaggtt accgtgtcac gtgacgggaa aaacaaccct gtcgtggatg tgtcagaact
     5701 gatccgtgta tacggaaatg tgaatatccc tgagaaacaa attcccggca tttcacaccg
     5761 tgaaaataca caaaaaaatt tcccacagga acagcttctg gcaatgcaaa aggaactggc
     5821 cgatctccgg caggctgtca ccctgatgct ggaagataaa acatcccggg aagaagaacg
     5881 ccgccagcac gatgatgagc gccggaaact ccaggccgaa gtggacagat tgacaacaga
     5941 actgacacag aagaaaaaac gcttctggtc cggctggttt agctgagaac ttaccgaagg
     6001 gaatggagaa catgacgaag caggaaaaaa ctgccctcaa tatggctaaa ttcattaagg
     6061 atcagtcgct gctgttgctg gaaaagctga acgagctgga catggatacc ccggccgaca
     6121 tgtgcgaacg gcttcacgag gatgcggaaa cactctggca ggtgatgcgg gataagctcg
     6181 gggaagaata acagcaccgg ccatgccggt gctggcgtta ttttttgccg aacggccacc
     6241 accggcgggc gggtttgtcc tccggcagtt caggcaccgg cacgttaaaa tagcggctgt
     6301 tatattcccg catcagctcc gctttcagct tctcgttcac cgcgctttcc ccgtgccgct
     6361 cgctgaccac ctcaatcatc gcgttaatct gctccggtga gcacttcccg gcatcaacca
     6421 cctgcacacc gttctcgatg gcgaaccgct ccacctttcg atagaggtaa tcacacagct
     6481 tcattcctgt gggtttgtcc cgttcccatt gcggattttc accggtgaaa aagtacgtgg
     6541 ctttttcgtc gctgttgagc cgctcgtcat acatcccgat ggatttcatc atcgccagat
     6601 gctgggcccg gtcgacaaaa cagaaaacat acgccccggt ctgccaggcg agcaccacca
     6661 tgtcttccag atgctcgctg gttggcataa tgcagcgctc gccgtctttg tcttccacgt
     6721 cctcaataaa taccatgtac agcgccataa tccctcactc cgttgtcata aatttaaagc
     6781 acgcgaacag cctctcccgg tccagctcca ccgttttgcg ggcctcgccg gtctgtggat
     6841 tcacgatttc atccaccgcc agaatcgccc gctgtacccg gacaagcgtc ggccggtcag
     6901 ggcagatgta ccagatttca tcgatgccgt tcgcccggcg gttgaacaga tacccggaca
     6961 ccacttcctg ataccgcctg cggctcttga cggtgcgctc tacctcaaat gccaccgttc
     7021 tgccgtccag ctccgccagc gcgtccggca ctttcatccc tttcagggcc atacggtgca
     7081 ggtaccgcca gccggtggcc ccccggccct ccatggagag gcgcagggac tgcacggcca
     7141 gccggtgcgg cagctgcgcc gccgacaccc ttgacggctg aaacggaata acgtccgtca
     7201 gcggctcgtc ctcgtcgaac gagaacgcca ccccgtgcgg cgtcagcccc cacacgctga
     7261 cctgacggcc gtcaaccggg tatttcgccc ggaccagcag ccctttcgcc accagactgt
     7321 ccaggaagcg gtaaaccggc tgtaccgccg caatccccag ccgtcgcgca accagctccg
     7381 ccgtggaaca cagctcttcc cgcagccagc gcagggtcag ccgtgttttc tccgcgttcc
     7441 gcgcctgccg cttcgcgtgg ctgacgttgt actgctccag tgtcatcagt aaagccccgc
     7501 aaaatcatca tccgcttttg gtccgccgcc gtcggccgga ggcaggtact ccgccagcgg
     7561 gtccgttttc gccagcaccg ggaaggtttt cagcgaaatt tcccggcgct caacccgcag
     7621 cgggctgctg aacgccagcc gcgccacgcc cagcccggtc atcaccgcga acccgtcagg
     7681 caggtgttgc agggtgttca cgtcaaacag cggccgcgcc tccttctgga tgaagctgtc
     7741 gccgctgatg tgctccacgt tccccgcctc ccggctggcc cggcggcgct ccacgtccac
     7801 ccggatctcc ccggtctggc ccgccgccca ctgcgcggac tcctggctgg ccgcgcgata
     7861 gaaccagcgg atcggcgtgt tatccagtac cgtggttttc acaaaatcag cgggcagatc
     7921 ctgcccgcac tgaccgaagt cgcccagcga ctggtgcgcg agcagcaggt tgcagtcccg
     7981 gctgcgcagc gtccccagcg cgttcagcac gtacttcgac agcagatatt taatctcatc
     8041 gagcatgatg ctcgcgtgcg gccacctccg gaactcatcc cgcgcaatga tgatttgcgc
     8101 gcagcgggca aacagcatct tctgcacccg gatcaccgcc tcatcgtcca tcgagccgat
     8161 gacgtacagg cagccgccgc cgttgattat ccccgccaca tcgataccgc tgtccgtctg
     8221 caggacgctc aggtcggcca cgttctccag cttggtgatc agccccttca cgtccttttt
     8281 cagggcctcc ggcaacgcgt acgccgccgc cagcacctgc tgaatgtttg ccccctgcgg
     8341 gaactgctcc gcaatcagct tcgccgcttt ctgctcgctg atgcggtagt ggtccgccac
     8401 gtcgccggtc tcactcaggt taaacccggc aaccagcaga ttcttcaggg cgtactggtc
     8461 gagatcgcgg aacagattca gctgcgccgg tttccccggc cgcagatcca gcagggtgaa
     8521 gtccgggcag tgcgcccgga agacgtgcgg cgcccacgcg tcccctttgg ggtcgaagta
     8581 caccacagcg tccccgaaat cgcgtacgca gcgaatgagc gcgttggtgg ccatcgcgct
     8641 cttgccgctc ccgggcaggc cgaggatctg aatattggtc ttgcgccagg tatcacgagg
     8701 gaccagcacc ggctggccct gctcatcctt acccagaaac accgcctgct gtttccgggc
     8761 ctgctccgtg gaaaaatcgg ccagcgcgtt aaccggtacc ggctccccga aatgcaggtg
     8821 gcggacgtct gtcatttctc cggtccgctg gctgctgcgc cggctctcct gattcagttt
     8881 cgcgttcacc gcctcgtcag cggccgcaaa atgaccatca agcagcagga acgccagcag
     8941 ccgcgccaga aggacgccgg cgacaagccc tccggcacac caggctatcc cgaacaggtt
     9001 agccggctgc agcaggtaat aatctgcgtt cgcatagagg tccgggcgca ggacggtttc
     9061 tccccagctc tcgataaaca cggcgacaac cagccccccc agaagggcca gaccggcagc
     9121 gccggtcagc cagtacgcgt agttccagac atgtcgccgt gccctgacca tccgcccggg
     9181 tgaatccagc gaggccagca ccgaaaatgg cacagtccgg ttattatcgc gcttatagcg
     9241 atcggcatac tcccgccgcg ccttatggct cagccccaca aaacgcattc ggccggccca
     9301 gagttcagcc agaccgaaaa acaggccgat aaccagaaaa aaaccggcca gactgtaata
     9361 ctcccggtac agccagctga cccacgggta aatctgccgg gccagctccg ggatatgcca
     9421 ctgccaggca agcgaatccg tattaaacat gcaatctctc tccttcagac gacgggttta
     9481 ccggcgtgat gcgcagcccc cgctgcacaa gccgcggaca tgccccaagg catggcggcg
     9541 gcggtcacgt ccgtggtgat tgcgttcagc tttcaccgcg gaggccagcc tctttccgcc
     9601 ccatgcggaa aaggcgcagc cccggaggaa caacgagcac gccccagcgt gtgagaggaa
     9661 aaggcggcac cccagtgcgg tcttttccgg tgttccggcc cccgtcagag ttcgccatag
     9721 cgagcggctg accacccgca aaatcaggag cagctttgct gcttcgtcag gtgcgtccag
     9781 caccgtggat ttttgtgggt ggtcaggcgt gagcctgacg ggggccgggt gaatttctgg
     9841 cgtggccggc gtccagccgg acagagccag aaagagggga aaagaccgtg cgtcggtggc
     9901 gtccagccag cgtaagcaag gtcttttccc ctgaagtcag ccacgcccca gcggggatga
     9961 cttgcatttc tccgcaagga gaaaaaagca acagagatag gttaacgtaa aatgttcagc
    10021 gaaaaaccga catggttcac ctatcctgat aattgatcgt cagggaatag aaagacttaa
    10081 tcaggggaca atatcccaca tcagcgcgtc cggtgaccgt cttgcaggtg ccggggaaat
    10141 tgcgcctcct cgctcactcg gtcgctacgc tccgggcgtg agactgcggc gggcgctgcg
    10201 gacacataca aagttaccca cagattccgt ggataagcag gggactaaca tgtgaggcaa
    10261 aacggcaggg ccgcgccggt ggcgtttttc cataggctcc gccctcctgc cagagttcac
    10321 ataaacagac gctttttcgg tgcatctgtg ggagccgtga ggctcaacca tgaatctgac
    10381 agtacgggcg aaacccgaca ggacttaaag atccccaccg tttccggtag gtcgctccct
    10441 cttgcgctct cctgttccga ccctgccgtt taccggatac ctgtccgccg ttctcccttg
    10501 cgggaagtgt ggcgctttct catagctcac acactggtat ctcggctcgg tgtaggtcgt
    10561 tcgctccaag ctgggctgta agcaagaact ccccgttcag cccgactgct gcgccttatc
    10621 cggtaactgt tcacttgagt ccaacccggg aaagcacgca aaacgccact ggcagcagcc
    10681 attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg ctcttgaagt
    10741 gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc
    10801 agttaccacg gttaagcagt tccccaactg acttaacctt cgatcaaacc acctccccag
    10861 gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat
    10921 cctttgatct tttctactga accgcgatcc ccgtcagttc agaggaggac gatggtgcca
    10981 ccgttccgcc ctgaacatcc cggatataat ttgcctgaca tgcggccaga agaaagccag
    11041 caacaatcaa tataaaaaga aaaatagtct ttaccttttt catatacttt ccctgtattt
    11101 aaagactccg gcattcatcc ctgaacaccg gaagtcagta tttcaaatca tccttgataa
    11161 ttttttgtta ccagttaccg tcccgataat caaaagctat ataacaatta tccacatcag
    11221 gttttatttg atttttaaaa aatggctgta gtaaaggaac ccattctttc ttcaaatcaa
    11281 actcaccatt attaatatta tcctttaaag gcattcccaa gctttcaatg acagaaccat
    11341 cgtcaccaaa gtctttagaa tactcttccc ctttaaactc ttcagttttc ttatcaaacc
    11401 atgtcaaacg taatttaagc cccatattta cctctcaaag atatttttta atgttccgtt
    11461 ttggatctgg ccctttaacc tgcttacccg ttttgggatc aaatgcgcct aggtgttcgc
    11521 catcactggc acgataccct tcaagctcac cgtgctggga atcccattca taaattttac
    11581 gttttttatc gccataccat cgggcccgct taccaccacc accttgttta ggtgttttag
    11641 gatcacctcg ctttaactct ccaagtccct ttatgtcttc tgttttaggt actggatgat
    11701 aatcatggcc gtagtcttta gtccccttgc gaggcttgtt cttttcctca tttaacttat
    11761 tttctgcatc cttcgctttt ttctcctttt gtttccggct ctccatcgcc ccggtaagtg
    11821 cagccttatc tctggataag gattcattca ttgcgtttaa agatgcctga gccgctttta
    11881 atttctcatt ctgattctga atatccttat caagatagcg aacctgatcc tgccacccgg
    11941 agccatattc atgaggcggg ttataagtca caaaatcagg taatgactcc aatttactga
    12001 tagtgtttta tgttcagata gtgcccgatg actttgtcat gcagctccac cgattttgag
    12061 aacgacaggg acttcctgcc cagccttgcc agatgctgtc tcagattcag gttgtgtcgc
    12121 tcaattcgct gcgtgtaacg cttactgatg acgtgcagtt ttcccttcag acgtgactca
    12181 tacatcggcc agccgtccgt catccatacc accacgtcaa aaactgacag caggctcaga
    12241 agacgctcca gtgtggccat agtgcgttca ccgaaaacgt gggctaccac cgccctgcgt
    12301 atcctgtcat acgcgtaaaa cagccagcgc tgacgtgatt ttgcaccgac gtagccccat
    12361 tgctcgtcca tctccgcaca gacaatgaca tcactgcccg gctgtatccg ggaggttact
    12421 gactgcggcc tgagtttttt aaatggcgca aaatggtgtt gaggcccacg cccattagtc
    12481 gtgcggtggc acggcaaccg acgccgttca tagccatatc aatgattttc tggtgtgtac
    12541 cgggctgaga agcggcataa gtgaaggtga gctgccatgt tttacggcag tgagagcaga
    12601 gataacgttg atgcccggca gtacttttgc cattacgcat cacgccttca gtagcggaac
    12661 aggagggaca gcagacggag actgaagcca cgggagcacc tcaaaaacac cattatacac
    12721 taaatcagca agttggtagc atcacc
//
