LOCUS       NZ_CP036332             8504 bp    DNA     linear   CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain BA28434 plasmid punnamed_3_VBA28434.
ACCESSION   NZ_CP036332
VERSION     NZ_CP036332.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08389138
            Assembly: GCF_002909775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8504)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Venkatesan,M. and
            Narasimman,V.
  TITLE     Klebsiella pneumoniae BA28434 hybrid assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8504)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Venkatesan,M. and
            Narasimman,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2019) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil nadu 632004, India
REFERENCE   3  (bases 1 to 8504)
  AUTHORS   Veeraraghavan,B. and Shankar,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2018) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method :: Canu v. 1.7; Unicycler hybrid v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 47.4x
            Sequencing Technology :: Oxford Nanopore MiniION; IonTorrent
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 22:44:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,666
            CDSs (total)                      :: 5,539
            Genes (coding)                    :: 5,312
            CDSs (with protein)               :: 5,312
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 139 of 227
            Pseudo Genes (incomplete)         :: 94 of 227
            Pseudo Genes (internal stop)      :: 23 of 227
            Pseudo Genes (multiple problems)  :: 26 of 227
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036332.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8504
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BA28434"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="punnamed_3_VBA28434"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2017"
                     /collected_by="Christian Medical College, Vellore, India"
     gene            <1..330
                     /locus_tag="C3E96_RS28840"
                     /old_locus_tag="C3E96_028840"
     CDS             <1..330
                     /locus_tag="C3E96_RS28840"
                     /old_locus_tag="C3E96_028840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_057056220.1"
                     /translation="SRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNAVVLWNTI
                     YMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLRPLKEASE
                     AENVA"
                     /name="Tn3 family transposase"
     gene            1186..1410
                     /locus_tag="C3E96_RS28850"
                     /old_locus_tag="C3E96_028850"
     CDS             1186..1410
                     /locus_tag="C3E96_RS28850"
                     /old_locus_tag="C3E96_028850"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071653266.1"
                     /translation="MSNKDSLERKVTPRKNEPVFPMALRVGIAEDICFLELVDFPEKES
                     GRVFYSIALTKKHAKNLIDGLNKFVESGD"
                     /name="hypothetical protein"
     gene            1412..2056
                     /locus_tag="C3E96_RS28855"
                     /old_locus_tag="C3E96_028855"
     CDS             1412..2056
                     /locus_tag="C3E96_RS28855"
                     /old_locus_tag="C3E96_028855"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044816498.1"
                     /translation="MKYINHCDSEDYYITNDSIDLGVRYDVSDYNKLELNDLRDPDLFK
                     VLGRAFVVVENSVWESWNDDVKTLDCSGDGYESILKHYESLELSGEFLLFFKKIRSAIS
                     VYNREYEFCTGDLEPSLQAIVQLIRYAPELAAKKDAQIYFDKKSECFGLIYKQTKKSKG
                     TLNLIVKTNNEIIFSFVRKVSGVVKVTGRAYIGDSLDNSIAIKSLFKMMEW"
                     /name="hypothetical protein"
     gene            2056..2532
                     /locus_tag="C3E96_RS28860"
                     /old_locus_tag="C3E96_028860"
     CDS             2056..2532
                     /locus_tag="C3E96_RS28860"
                     /old_locus_tag="C3E96_028860"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_077861907.1"
                     /translation="MTVLSTLNNLKSIPCINAFTGRANNSYLWRYVMEDHKDEFGATTI
                     KCFYLREEISEDYVSIYHSNKDYYEGMVRDVHKRIPVGKTKPAIYLSFDAAEVIEQVNK
                     PDKCIDIKDEGYPHLGLWYLFDINDEFTLIEVTTTILELSSFFQRNEGGVVVPL"
                     /name="hypothetical protein"
     gene            complement(2736..2848)
                     /locus_tag="C3E96_RS30900"
                     /old_locus_tag="C3E96_028865"
                     /pseudo=""
     CDS             complement(2736..2848)
                     /locus_tag="C3E96_RS30900"
                     /old_locus_tag="C3E96_028865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3262..3453
                     /locus_tag="C3E96_RS28875"
                     /old_locus_tag="C3E96_028875"
     CDS             3262..3453
                     /locus_tag="C3E96_RS28875"
                     /old_locus_tag="C3E96_028875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_044816495.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSGLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3453..3875
                     /locus_tag="C3E96_RS28880"
                     /old_locus_tag="C3E96_028880"
     CDS             3453..3875
                     /locus_tag="C3E96_RS28880"
                     /old_locus_tag="C3E96_028880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_044816494.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKNTKVVD
                     TSELIRVFGSLYGGGDTVKHDVKGDAGATGVNISGLHDLQHKIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKLEPSPQVHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4068..4928)
                     /locus_tag="C3E96_RS28885"
                     /old_locus_tag="C3E96_028885"
     CDS             complement(4068..4928)
                     /locus_tag="C3E96_RS28885"
                     /old_locus_tag="C3E96_028885"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(5111..5668)
                     /locus_tag="C3E96_RS28890"
                     /old_locus_tag="C3E96_028890"
     CDS             complement(5111..5668)
                     /locus_tag="C3E96_RS28890"
                     /old_locus_tag="C3E96_028890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            5832..>8504
                     /locus_tag="C3E96_RS28895"
                     /old_locus_tag="C3E96_028895"
                     /pseudo=""
     CDS             5832..>8504
                     /locus_tag="C3E96_RS28895"
                     /old_locus_tag="C3E96_028895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /name="Tn3-like element Tn3 family transposase"
     ARG             complement(4072..4928)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(2737..3141)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.902"
                     /coverage="99.0"
                     /evalue="3.5400000000000005e-173"
ORIGIN
        1 agtcgccatg ccgttgccag agccatctgt cacggtcaaa aaggtgagat aagaaaacga
       61 tataccgacg gtcaggaaga tcaactgggc acactggggc tggtcactaa cgccgtcgtg
      121 ttatggaaca ctatttatat gcaggcagcc ctggatcatc tccgggcgca gggtgaaaca
      181 ctgaatgatg aagatatcgc acgcctctcc ccgctttgcc acggacatat caatatgctc
      241 ggccattatt ccttcacgct ggcagaactg gtgaccaaag gacatctgag accattaaaa
      301 gaggcgtcag aggcagaaaa cgttgcttaa cgtgagtttt cgttccactg agcgtcagac
      361 cccagttcct gaaccttccc aaaaatcgcc agatctctga acgctcctca ccactaaaaa
      421 tatccacacc ccacgcaaaa ttaaaatttt gcagatttgt attttatatc agtagtttac
      481 gttctataaa tttcgttttt ttaattttat ttatacagat ccgactctat gacgactcgt
      541 catagagtcc ccggcgagtc gtcatagagt cggtaacgac tcaggactat aaaaacaata
      601 agttaacaac gacccgactc tagacccgac tccccgtttg gttgtttttt agacgtgacg
      661 actctgggta cgactctagt gaagagtcgc cggtgaatcg tgtggtgagt cgtccggcga
      721 cttgccataa cgtgattgcc ggcctcgacg aacccgattc ctgaacgtgc tgaaaaccgg
      781 ttttccggct gcgccggcaa cgcttcacgg ccacgcaata aattgcgtcc ccgttcaccg
      841 ttttcggatg ggaagaacgc cggaaacagg acttaccctg gctgaatggc gtgaattttc
      901 gcgctatgca cttgcgtgca tactcatgca tgccgtaaaa acagagcctg cgcgtttctg
      961 gcgggttttc gggtggtttg ttgcctattt taccggtttc ccgtcagaaa cgccctgagg
     1021 ccgttttagc ggtgcgtaca attttggtgt tatgttaaat catggccaca aaattccata
     1081 aagtgaccaa tgtaatgtta aatgagcatt gtggatgaaa gatgttgttt tatggtatta
     1141 ttttttatgt tctaaactta aatttatttc tatcggggta ttataatgag caataaagat
     1201 tctttagaaa gaaaagtaac accaagaaaa aatgaacctg tattcccgat ggctctaaga
     1261 gttgggatag ctgaagatat ttgtttttta gagttagtgg actttcctga aaaagaatca
     1321 ggaagggttt tttattccat agctctaacc aaaaaacatg caaagaacct tatcgatgga
     1381 ttgaataaat tcgttgagtc aggtgattag tatgaaatac attaaccact gcgatagtga
     1441 agattattat attactaatg atagcattga tctcggtgtt cgttatgatg tttctgatta
     1501 taataagtta gagctaaatg atttaagaga tccggatttg tttaaagttt tgggtcgagc
     1561 atttgttgtt gttgagaatt ctgtttggga gtcttggaat gatgatgtta aaacattaga
     1621 ttgctctggt gacggttatg agtcaatttt aaaacattac gaatcattag aattatcagg
     1681 tgaattctta ttgtttttta aaaaaataag aagtgccatt tctgtttata atagagaata
     1741 cgagttctgc actggtgacc tagaaccatc tttgcaggct attgttcagt tgattaggta
     1801 tgcccctgaa cttgctgcaa aaaaagacgc tcagatttac tttgataaaa aatcagagtg
     1861 ttttgggctt atatataaac aaactaagaa aagcaaaggc acgctaaatt taatagtgaa
     1921 aacaaataac gagattattt tttcctttgt tagaaaagtt tctggagtgg ttaaggttac
     1981 aggtagggca tatattggcg atagccttga taactccatt gctatcaaat cattatttaa
     2041 aatgatggag tggtgatgac tgtgttatca actttaaata acctcaaatc aatcccttgt
     2101 attaatgcat ttacgggcag agcaaataat tcatatttat ggcgttatgt tatggaagat
     2161 cataaagatg agtttggcgc tacaactatt aagtgttttt atcttaggga ggaaatttca
     2221 gaggattatg tgtctattta tcattcaaat aaagattatt atgaaggaat ggtaagggat
     2281 gtacataaaa gaatccctgt agggaaaacc aaacctgcca tatatctctc atttgatgca
     2341 gcggaagtga tcgagcaagt taacaaacca gataaatgta ttgatataaa ggacgaaggt
     2401 taccctcact tgggtttgtg gtatttattt gatattaatg atgaatttac tttgattgag
     2461 gtgactacaa ctattttgga attatcctct ttttttcaaa gaaatgaagg tggcgtggtt
     2521 gttcctcttt agcgaaaacc gtcttgacgt catcaagtgt gacctagtac tcttcttgaa
     2581 aaagaaaaac cgcctttgcg gggcggtttt tcttaggttc taagagtttg cggctctatg
     2641 aaccaaggta actggcttag aggagcgcag taaccaaaat cgttctttca gtttagcctt
     2701 aactggcaca taacttcaag actaactcct ctaaatcagt taccagtggc tgctgccagt
     2761 ggcgcttcgt cgtgtctttc cgggttggac tcaagtgata gttaccggaa aaggcgcagc
     2821 agtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg
     2881 aactgagtgt caggcgtgga atgagaaaac gcggccataa cagcggaatg acaccggtaa
     2941 accgaaaggc aggaacagga gagcgcacga gggagccacc agggggaaac gcctggtatc
     3001 tttaagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc
     3061 aggggggcgg agcctatgga aaaacggccg ctgtgcggcc tctccttctc cccatcacgc
     3121 gatcgctcgg ttttctcgag cttttatacg catgctcctg ccgctcgccg cagccgaacg
     3181 gcaggagcgt agcgactgag tgagcgagga agcggaaaag agactagttt gacactgagc
     3241 actgacgctc tgaggcctct tatgacgaaa caacagaaaa cagcgctgaa catggctaaa
     3301 ttcatccaga atcagtctct gctcctgctg gagaagctta acgagcttga tcttgatgta
     3361 gaggccgacc tttgtgagaa actccacgat gatgcggagc atcttttccg tacactgtct
     3421 tcgggactgg atgaattaca ggatggaaac tgatggccag agtgtctatt agtgaagcgg
     3481 ctcggcttgt taaggttagt cgccccacaa tttacaagat gattaacagt gggaaactaa
     3541 gttacacctc tgttgtaaag catgggaaaa atactaaagt tgttgataca tcagagctta
     3601 ttagggtttt tggctcgctt tatggtgggg gcgacactgt aaaacatgat gtaaaaggtg
     3661 acgcaggcgc tacaggggtt aacatctctg gtttacatga tttacagcat aaaattgctt
     3721 tgcttgaagc tgaaaacgat ggtcttaaag gggctgttaa agctcgtgat gaacatatcg
     3781 attctcttcg ccaggctatg cagcttctgg aacacaagct cgaaccctct ccacaggttc
     3841 attctccgtg gtggaaattc tggaagaaat cctgaccccg tcatgcttca gttttcctct
     3901 ccggccattc cggggagttc ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3961 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4021 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4081 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4141 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4201 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4261 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4321 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4381 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4441 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4501 ggtcctccga tcgttgtcag aagtaagttg gcagcagtgt tatcactcat ggttatggca
     4561 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4621 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4681 tcaacacggg ataataccgc accacatagc agaactttaa aagtgctcat cattggaaaa
     4741 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4801 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4861 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg aaaatgttga
     4921 atactcatac tcttcctttt tcaatattat tgaagcattt accagggtta ttgtctcatg
     4981 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     5041 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     5101 aataggcgta tcacgaggcc ctttcgtctt caagaatttt ataaaccgtg gagcgggcaa
     5161 tactgagctg atgagcaatt tccgttgcac cagtgccctt ctgatgaagc gtcagcacga
     5221 cgttcctgtc cacggtacgc ctgcggccaa atttgattcc tttcagcttt gcttcctgtc
     5281 ggccctcatt cgtgcgttct aggatcctcc ggcgttcagc ctgtgccaca gccgacagga
     5341 tggtgaccac catttgcccc atatcaccgt cggtactgat cccgtcatca atgaaccgga
     5401 ctgccacgcc ctgagcgtca aattccttta tcagttggat catatcggca gtgtcgcggc
     5461 caagacggtc gagcttctta accagaatga catcaccttc ctccaccttc atcctcagca
     5521 aatccagccc ttcccggtct gttgaactgc cggatgcctt atcggtaaat atacggtttg
     5581 ctttcacacc tgcgtctttg agtgctctga cctgaagatc aagagactgc tgactggttg
     5641 agacccgagc gtaaccaaaa agtcgcataa aaatgtacct taaatcgaat atcggacaac
     5701 tcatgtctat tattacaaat ttacgattta atagacatat taatgtaaca gttttacgat
     5761 gtccgataat ttataacatt tcgtacggtt ggaaaaatgt tactaaatgc ccgtcaggca
     5821 gggaggccga tatgcccgtt gactttctga ccactgagca gactgaaagc tatggcagat
     5881 tcaccggtga accggatgag cttcagctgg cacgatattt tcaccttgat gaagcagaca
     5941 aggaatttat cggaaaaagc agaggtgatc acaaccgtct gggcattgcc ctgcaaattg
     6001 gatgtgtccg ttttctgggc accttcctca ccgatatgaa tcatattcct tccggcgtcc
     6061 ggcattttac cgccagacag ctcgggattc gtgatatcac cgttcttgca gaatacggtc
     6121 agagggaaaa tacccgccgt gagcatgcag cgctgatacg tcagcactat cagtatcgtg
     6181 aatttgcctg gccctggaca tttcgcctta cccgtctttt atatacccgg agctggataa
     6241 gcaacgaacg tcctggcctg cttttcgatc tggcgacagg gtggcttatg caacatcgta
     6301 ttattctccc cggagccact acgctgaccc ggttgatttc agaggtaagg gaaaaggcga
     6361 cgttgcgcct gtggaacaaa ctggcactga taccgtcagc cgaacagcgt tcacagctgg
     6421 agatgctgct ggggccaact gattgcagcc gcctgtcttt actggaatca ctgaaaaagg
     6481 gccctgtgac catcagtggt ccggcgttta atgaagcaat tgaacgctgg aaaactctga
     6541 acgattttgg cctgcatgct gaaaacctga gtacactccc ggctgtgcgc ctgaaaaatc
     6601 tcgcacgtta tgctggtatg acttcggtgt tcaatattgc caggatgtca ccgcagaaaa
     6661 ggatggcggt tctggttgcc tttgtccttg catgggaaac gctggcgctg gatgatgcat
     6721 tggacgttct ggacgccatg ctggccgtta tcatccgtga cgccagaaag attgggcaga
     6781 aaaaacggct ccgctcgctg aaggatctgg ataaatctgc attggcgctc gccagcgcat
     6841 gttcgtacct gctgaaagaa gaaacaccgg acgaatcgat tcgtgctgag gtgttcagct
     6901 acatcccaag gcaaaagctg gctgaaatca tcacgcttgt ccgtgaaatt gcccggccct
     6961 cagacgataa ttttcatgaa gaaatggtgg agcagacggg cgcgttcgtc gtttcctgcc
     7021 ccatctgctg aataccgtta aattttcatc cgcacctgcc ggggttacca ctctgaatgc
     7081 ctgtgactac ctcagccggg agttcagctc acggcggcag ttttttgacg acgcaccaac
     7141 ggaaattatc agtcggtcat ggaaacggct ggtgattaac aaggaaaaac atatcacccg
     7201 caggggatac acgctctgct ttctcagtaa actgcaggat agtctgaggc ggagggatgt
     7261 ctacgttacc ggcagtaacc ggtggggaga tcctcgtgca agattactac agggtgctga
     7321 ctggcaggca aaccggatta aggtttatcg ttctttgggg cacccgacag acccgcagga
     7381 agcaataaaa tctctgggtc atcagcttga tagtcgttac agacaggttg ctgcacgtct
     7441 ttgcgaaaat gaggctgtcg aactcgatgt ttctggcccg aagccccggt tgacaatttc
     7501 tcccctcgcc agtcttgatg agccggacag tctgaaacga ctgagcaaaa tgatcagtga
     7561 tctactccct ccggtggatt taacggagtt gctgctcgaa attaacgccc ataccggatt
     7621 tgctgatgag tttttccatg ctagtgaagc cagtgccaga gttgatgatc tgcccgtcag
     7681 catcagcgcc gtgctgatgg ctgaagcctg caatatcggt ctggaaccac tgatcagatc
     7741 aaatgttcct gcactgaccc gacaccggct gaactggaca aaagcgaact atctgcgggc
     7801 tgaaactatc accagcgcta atgccagact ggttgatttt caggcaacgc tgccactggc
     7861 acagatatgg ggtggaggag aagtggcatc tgcagatgga atgcgctttg ttacgccagt
     7921 cagaacaatc aatgccggac cgaaccgcaa atactttggt aataacagag gatcaccggt
     7981 acaactttgt gtccgatcag tattccggct ttcatggcat cgttataccg gggacgctga
     8041 gggactctat ctttgtgctg gaaggtcttc tggaacagga gaccgggctg aatccaaccg
     8101 aaattatgac cgatacagca ggtgccagcg aacttgtctt tggccttttc tggctgctgg
     8161 gataccagtt ttctccacgc ctggctgatg ccggtgcttc ggttttctgg cgaatggacc
     8221 atgatgccga ctatggcgtg ctgaatgata ttgccagagg gcaatcagat ccccgaaaaa
     8281 tagtccttca gtgggacgaa atgatccgga ccgctggctc cctgaagctg ggcaaagtac
     8341 aggtttcagt gctggtccgt tcattgctga aaagtgaacg tccttccgga ctgactcagg
     8401 caatcattga agtggggcgc atcaacaaaa cgctgtatct gcttaattat attgatgatg
     8461 aagattaccg ccggcgcatt ctgacccagc ttaatcgggg agaa
//
