LOCUS       NZ_CP036331            10101 bp    DNA     linear   CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain BA28434 plasmid pColKP3.
ACCESSION   NZ_CP036331
VERSION     NZ_CP036331.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08389138
            Assembly: GCF_002909775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Venkatesan,M. and
            Narasimman,V.
  TITLE     Klebsiella pneumoniae BA28434 hybrid assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10101)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Venkatesan,M. and
            Narasimman,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2019) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil nadu 632004, India
REFERENCE   3  (bases 1 to 10101)
  AUTHORS   Veeraraghavan,B. and Shankar,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2018) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method :: Canu v. 1.7; Unicycler hybrid v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 47.4x
            Sequencing Technology :: Oxford Nanopore MiniION; IonTorrent
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 22:44:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,666
            CDSs (total)                      :: 5,539
            Genes (coding)                    :: 5,312
            CDSs (with protein)               :: 5,312
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 139 of 227
            Pseudo Genes (incomplete)         :: 94 of 227
            Pseudo Genes (internal stop)      :: 23 of 227
            Pseudo Genes (multiple problems)  :: 26 of 227
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036331.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BA28434"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pColKP3"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2017"
                     /collected_by="Christian Medical College, Vellore, India"
     gene            <1..349
                     /locus_tag="C3E96_RS28775"
                     /old_locus_tag="C3E96_028775"
     CDS             <1..349
                     /locus_tag="C3E96_RS28775"
                     /old_locus_tag="C3E96_028775"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MbeD/MobD family mobilization/exclusion protein"
                     /protein_id="WP_131086846.1"
                     /translation="DRAEGVLNDGIGQELLCAFEALQSAYEQQHKAWQESYANLASMYE
                     STKQRGRDQQGSSRAIDEAGERLNAASGRQSKQLEGLTGLVSKLQRKEHRLLKALDRGW
                     ERGDRSRGFER"
                     /name="MbeD/MobD family mobilization/exclusion protein"
     gene            970..1767
                     /locus_tag="C3E96_RS28780"
                     /old_locus_tag="C3E96_028780"
     CDS             970..1767
                     /locus_tag="C3E96_RS28780"
                     /old_locus_tag="C3E96_028780"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            complement(2174..>2326)
                     /locus_tag="C3E96_RS28785"
                     /old_locus_tag="C3E96_028785"
                     /pseudo=""
     CDS             complement(2174..>2326)
                     /locus_tag="C3E96_RS28785"
                     /old_locus_tag="C3E96_028785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            2702..3649
                     /locus_tag="C3E96_RS28790"
                     /old_locus_tag="C3E96_028790"
     CDS             2702..3649
                     /locus_tag="C3E96_RS28790"
                     /old_locus_tag="C3E96_028790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            4055..4447
                     /locus_tag="C3E96_RS28795"
                     /old_locus_tag="C3E96_028795"
     CDS             4055..4447
                     /locus_tag="C3E96_RS28795"
                     /old_locus_tag="C3E96_028795"
                     /inference="COORDINATES: protein motif:HMM:NF037437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            4429..4767
                     /locus_tag="C3E96_RS28800"
                     /old_locus_tag="C3E96_028800"
     CDS             4429..4767
                     /locus_tag="C3E96_RS28800"
                     /old_locus_tag="C3E96_028800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4748..5512
                     /locus_tag="C3E96_RS28805"
                     /old_locus_tag="C3E96_028805"
     CDS             4748..5512
                     /locus_tag="C3E96_RS28805"
                     /old_locus_tag="C3E96_028805"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_131086847.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYRSV"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5436..5870
                     /locus_tag="C3E96_RS30675"
     CDS             5436..5870
                     /locus_tag="C3E96_RS30675"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_228261339.1"
                     /translation="MSKILDMAKDFEQSSKQQANAIEASDRRIHEARESYLRGVEIKRG
                     KMSAAIAEHSQKMGWLLLKTWIWMLVSIVLFLSATSGILWYQGELIAERQATLQALAEK
                     GGKLKLTQCGKKLEHLCVQISDPNPTVWGKDTYWIVPKGY"
                     /name="MbeB family mobilization protein"
     gene            5871..6143
                     /locus_tag="C3E96_RS28815"
                     /old_locus_tag="C3E96_028815"
     CDS             5871..6143
                     /locus_tag="C3E96_RS28815"
                     /old_locus_tag="C3E96_028815"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015060051.1"
                     /translation="MTGLERELLCAFEALQSAYEQQHKAWQESYANLASMYESTKQEAE
                     ISRAAAERLTRQVNGLTQQVDGLSKQLEGLTGLVSKLQKKGNTGS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6480..6725)
                     /locus_tag="C3E96_RS30155"
     CDS             complement(6480..6725)
                     /locus_tag="C3E96_RS30155"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123639735.1"
                     /translation="MSDAFGAKETAIASAKLLGKGVVNLAHFAATTGLDSVVKQTSNKV
                     LNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            7103..7906
                     /gene="blaOXA"
                     /locus_tag="C3E96_RS28825"
                     /old_locus_tag="C3E96_028825"
                     /pseudo=""
     CDS             7103..7906
                     /gene="blaOXA"
                     /locus_tag="C3E96_RS28825"
                     /old_locus_tag="C3E96_028825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /GO_function="GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family class D beta-lactamase"
                     /name="blaOXA"
     gene            complement(8298..>8450)
                     /locus_tag="C3E96_RS28830"
                     /old_locus_tag="C3E96_028830"
                     /pseudo=""
     CDS             complement(8298..>8450)
                     /locus_tag="C3E96_RS28830"
                     /old_locus_tag="C3E96_028830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            8846..9769
                     /locus_tag="C3E96_RS28835"
                     /old_locus_tag="C3E96_028835"
                     /pseudo=""
     CDS             8846..9769
                     /locus_tag="C3E96_RS28835"
                     /old_locus_tag="C3E96_028835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     ARG             971..1764
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2715..3636
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          8837..9756
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.565"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          4749..6196
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.265"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggatcgtgcc gaaggggtac taaatgacgg gattgggcag gaattgctct gcgcgttcga
       61 ggctttgcag agcgcatacg agcagcagca caaggcatgg caggaaagct acgcgaactt
      121 ggcgagcatg tacgagagca ccaaacaaag aggccgagat cagcagggca gcagcagagc
      181 gattgacgag gcaggtgaac ggcttaacgc agcaagtgga cggcagagca agcagctgga
      241 gggcttgacc gggctagtca gcaagttgca aagaaaggaa cacaggctcc tgaaggcact
      301 ggatcgaggc tgggagcgtg gagacagatc gcgaggcttt gagcgttgac ggaacaaaac
      361 ggccgtcagt tataaaaaaa ggaaagtgct atcacttttc cttttatcgg cgggaattat
      421 cgttgagttc ttctatgcca tttttcatag tagtaactgt aatcttcatc tctaccacca
      481 gattcaatcc agtcatcata ctcagggcta tttttgaaat ctgaatcaga catgctaact
      541 ccttgttatt agttggcttc tatggaagcg cacctctagt cccaccgtga agttatgttc
      601 aacgatgtag tccacattct cattgtgcgg cccctaagaa tatcatcaat aaaattgagt
      661 gttgctctgt ggataacttg cagagtttat taagtatcat tgcagcaaag atgaaatcaa
      721 tgatttatca aaaatgattg aaaggtggtt gtaaataatg ttacaatgtg tgagaagcag
      781 tctaaattct tcgtgaaata gtgatttttg aagctaataa aaaacacacg tggaatttag
      841 gtaatagtta aagcttgtgc ctgattgttg ccacaattgg tcagcagttt gctagggaat
      901 gagagggcag agaatgataa gcttagcact taatttgtat ggatatattg cattaagcaa
      961 ggggacgtta tgcgtgtatt agccttatcg gctgtgtttt tggtggcatc gattatcgga
     1021 atgccagcgg tagcaaagga atggcaagaa aacaaaagtt ggaatgctca ctttactgaa
     1081 cataaatcac agggcgtagt tgtgctctgg aatgagaata agcagcaagg atttaccaat
     1141 aatcttaaac gggcgaacca agcattttta cccgcatcta cctttaaaat tcccaatagc
     1201 ttgatcgccc tcgatttggg cgtggttaag gatgaacacc aagtctttaa gtgggatgga
     1261 cagacgcgtg atatcgccgc ttggaatcgt gaccatgact taattaccgc gatgaagtac
     1321 tcagttgtgc ctgtttatca agaatttgcc cgccaaattg gtgaggcacg tatgagtaaa
     1381 atgctgcacg ccttcgatta tggcaatgag gatatctcgg gcaatgtaga cagtttttgg
     1441 ctcgatggtg gtattcgcat ttcggctacc cagcaaatcg cttttttacg caagctgtat
     1501 cacaacaagc tgcacgtttc tgagcgtagt cagcgcatcg tgaaacaagc catgctgacc
     1561 gaagccaatg gcgactatat tattcgggct aaaacgggat actcgactag tatcgaacct
     1621 aagattggct ggtgggttgg ttgggttgaa cttgatgata atgtgtggtt ttttgcgatg
     1681 aatatggata tgcccacatc ggatggttta gggctgcgcc aagccatcac aaaagaagtg
     1741 ctcaaacagg agaaaattat tccctagaag tggttagcgc gtatttgtgt gaaatagccg
     1801 tcatataagc tgtaaagata tatggacaaa atgctgatgc tcgatgcgct tgtagttaat
     1861 ggctcatact taatcggtca ggcctaatcg acttaagctc cgcgcgttaa ctttcagcct
     1921 gagcattagc atcacattcc taaacgtaaa aagcctagcc aatttgctag gcttttttgt
     1981 tatctcaatg gccatggtca ctcaacatgg gggtgtgaat aaaggagtgg cgatgtcagt
     2041 aaaggtgaat gccgctgcga taaagtgcct agcccatcaa ggattgtagg ctttcttggc
     2101 tgctaaagcg tttgacgaaa aagtccaaga aggcgctgat attggttctc catcattttc
     2161 atctaccggt ttactatagg tcaaccaggc cgtccttgcc ggcgcttggg acgcagacga
     2221 tggcatcaaa tgcctcgctc aaattcgttt ctacagaggc gctttgggac cgcattcgct
     2281 ttgctttcat gggggcatct gtcaatgtca gacatgaatc ctccgatata ggttgatttt
     2341 tgatctcttc aacacttggt gttgtccgac tagagagact tatatggatc agtattcttg
     2401 cactgacaat ggtgccactc aatcccaagg atcgacctag ttagaggcgt agatcaagca
     2461 attcaaaaca gcgcagaagc agaaggaggt ggaaatatgt tccagggtga aaggttacat
     2521 gcaactaacg ttgctctcta ggatgtgcct gctgtaacgt attgtccaga aaagacaacc
     2581 ccccgttatc gcttgcatct tggcggagct gcgaaaaacg ggggccgtga cgaatcacaa
     2641 gagaagtcta tcacatggat catgccaaac aaccccacgc cgccaataag cgcggtggtg
     2701 cttgagaaag tgcgcacagc ttgagctgtt tcgggagcgc ctgccccgga aaccctacta
     2761 ttccgacgag cttacaacgg gcctacggat tgcggatgtt gcccgcgccc ttggtgcgcg
     2821 ttacatccag ccgaacgggc caacgcaccg ccactggatc gtttttgacg tagaccatgc
     2881 agccgctacg ttatcttggg atgacgtagg cgctccagcc cctaacatta ccgtcacgaa
     2941 caaggccaac ggccacgcgc atctgatcta cggactggat acgccgatcc gcactgcccc
     3001 ggatggaaat gcagcccctc tgcggtatgc cgccgccatc gaggccgcgc tccgggagaa
     3061 attaggggcg gatatgggct actccgggct gatctgcaag aaccccctgc atgagcactg
     3121 gctggtgcag gtatgggaac cccggctcta cgatttagcc tggctatccg attacctcga
     3181 tctgagctcc tacaatggcc gcaaatcgct tcctgagtac gggcttgggc gaaactgtac
     3241 cctgttcgag aaaacgcgcc tgtgggccta taaagcgatc cggcagggct ggccggatta
     3301 ccccgaatgg ttggccgctt gtgttgaccg ggcatcagga tataacgccc agttcgagca
     3361 gccgctcccc gcaaacgaag tccggcacac ggccaagtcc attgccaaat ggacgcacca
     3421 gcacctaagc cccgcagggt tcagagagga gcaggcccgg agaggtgcga agggcggcaa
     3481 ggtatccaag ggagggggaa ggccgagcaa tagcggtaag gacaagtccg atctattgcc
     3541 ggaggttctg cggcttaagg ctcaggggta caccaaccgc gatatagcgg acgatcttca
     3601 aatctccccc agtacggtga gcgtttacct aaagcgtgat cgtccgtgat cgaaacaagc
     3661 cttatcagat aatagccccc tttagggggg ttcctgcgct cttgtctttt agccttaaaa
     3721 aatcctttta gcgcggatct ttggcgaggt ggacaactgc gcctgtccgt ccatcgcccc
     3781 cctgtttttt cggggggtgt gggcggcagc agttatccac ggagccgctc tttcccttga
     3841 tcttgctcta gggcttgcag tatcccgccc atggttgaat aatgtactaa gcaaggctac
     3901 acagaggccc ctctacctgc ttcgcggtct cgggcctctg tggccttgtt cagggtgcta
     3961 cgccccccga aaccccctgt ggcccccgct cgatccgccc cttcgggacg gtctgcgcgg
     4021 tggcttcttg gctctggaga atggacgagg ccctatgtca acaacacaga tagagaaaga
     4081 ttcgagaagg aaggcagtca atggcgttat ttctagtttc caactagagg gggaggccat
     4141 aaccgatgaa ttcaaaattc tacttgaaga ttatatcaat ggaaaaattt ccaccgatga
     4201 ggtgttagct atggtaagtg acgaacccac aacaacggaa tacgactacg aaatcacccc
     4261 ccgcactctc ggtcttggcg ggggctggaa tctgcgtttg ctggagaacg gcgaagaggt
     4321 cggcgggggt gttttcccct tgccggagca ctgcgatttt cgggatgaaa aggcgctcca
     4381 gacgctactc gatagcctct atgaggacgc cttagcggag gcctctgcat ggctggccag
     4441 ccgttaaagc gcctccgctc tatcaagatc cgggtctcag atgcggagct tgagcgcctg
     4501 cgggagctct gccccaaggc ccaactggcg gagtggatgc gggagcaatg tctcggtgtt
     4561 gcgcaacccc agcgccgaac gccggcgcct accgtagatc cggcgctctt gcggcagttg
     4621 gccggaatgg gcaacaacct gaaccagatc gcccggcgcg ttaattccgg ggagtggggg
     4681 ccgttggatc ggctgcgcat catagcggag ctgtccgcta taggccgcga gctggaggaa
     4741 ctgcaccatg atcatcaaat tccataaccg tggggtaggc cggggctccg ggcctgttgg
     4801 ctacttgttg ggccgtgacg ggaaccggga aggcgcaacg ctcgaccggg gcaaccctgc
     4861 ggtgattgaa gccctgattg attccagtcc ctacgccaaa aaatacacct ctggcgttct
     4921 gtcgtttgcc gaggctgatt tacccagggc gaccaaggac aagctgatga gcgactttga
     4981 aaaggcgctc ctgcccggac tggagggcaa ccagtacgct gttttgtggg tagagcaccg
     5041 cgacaagggg cggctggagc tgaattttgt agtcccgaac atcgaattgc agagcgggaa
     5101 gcggctgcaa ccctacttcc acaaggccga caatccgcgc ctcgatgcgt ggcgaaccgt
     5161 ggtaaacggg gagctgaaat tgcacgaccc ggacgatccg ttaaaccgtc aggccgttat
     5221 cacgccaaag gatttgccga gggggcacaa ggaggccgct caggccatta cagacgggct
     5281 tttacgcatg gccggagcag gggagatcca gagccgcgat gatgtgcgca aagccttgca
     5341 gggtgcgggt ttcagcgtgg tacgcgaaac caaaaacagc ctgagcattg ccgacccgga
     5401 cggggggcgg aacatccgat taaagggggt gctgtatgag caagatttta gacatggcga
     5461 aggacttcga gcagagctcg aagcagcagg ccaacgctat cgaagcgtct gaccgaagaa
     5521 ttcacgaggc acgagaaagc tatctccggg gcgttgaaat caagcgaggg aaaatgagcg
     5581 ccgctattgc cgagcacagc cagaaaatgg gctggctgtt gctcaaaacg tggatctgga
     5641 tgctggtcag catcgtcctg ttcttgagcg caacgagcgg gatcctctgg tatcaggggg
     5701 aattgatagc ggagcggcaa gccactctcc aagccttggc agagaagggc ggcaagctaa
     5761 agctgaccca gtgcgggaag aaattggagc acctttgcgt gcagatctcc gaccccaatc
     5821 caaccgtttg ggggaaggat acctattgga tcgtgccgaa ggggtactaa atgacgggat
     5881 tggagcggga attgctctgc gcgttcgagg ctttgcagag cgcatacgag cagcagcaca
     5941 aggcatggca ggaaagctac gcgaacttgg cgagcatgta cgagagcacc aaacaagagg
     6001 ccgagatcag cagggcagca gcagagcgat tgacgaggca ggtgaacggc ttaacgcagc
     6061 aagtggacgg cttgagcaag cagctggagg gcttgaccgg gctagtcagc aagttgcaaa
     6121 agaaagggaa cacaggctcc tgaaggcact ggatcgaggc tgggagcgtg gagacagatc
     6181 gcgaggcttt gagcgttgac ggaacaaaac ggccgtcagt tataaaaaag gaaagtgcta
     6241 tcactttcct tttatcggcg ggaattatcg ttgagttctt ctatgccatt tttcatagta
     6301 gtaactgtaa tcttcatctc taccaccaga ttcaatccag tcatcatact cagggctatt
     6361 tttgaaatct gaatcagaca tgctaactcc ttgttattag ttggcttcta tggaagctag
     6421 cgcaccgtga agttatgatc aacgatgtag tccacatcat tgtcgaaatg tgcgttcagc
     6481 tagtcttcgg tgttttctgc tttatttcta gcggcgattc tcgcgtctgc ccatgcggca
     6541 gactttttag cctgttctcg ttgttcttcg gttgaagact cgctgttgag aactttgttg
     6601 ctcgtttgtt tgaccaccga atccaaacct gttgttgctg caaaatgagc taggttaaca
     6661 actcccttac ctaacaactt ggcactagcg attgcggttt ctttagcccc aaaagcatca
     6721 gacacctcct caagcgcttt agataggtta aaaatcccat actcccctaa gaatatcatc
     6781 aataaaattg agtgttgctc tgtggataac ttgcagagtt tattaagtat cattgcagca
     6841 aagatgaaat caatgattta tcaaaaatga ttgaaaggtg gttgtaaata atgttacaat
     6901 gtgtgagaag cagtctaaat tcttcgtgaa atagtgattt ttgaagctaa taaaaaacac
     6961 acgtggaatt taggtaatag ttaaagcttg tgcctgattg ttgccacaat tggtcagcag
     7021 tttgctaggg aatgagaggg cagagaatga taagcttagc acttaatttg tatggatata
     7081 ttgcattaag caaggggacg ttatgcgtgt attagcctta tcggctgtgt ttttggtggc
     7141 atcgattatc ggaatgccag cggtagcaaa ggaatggcaa gaaaacaaaa gttggaatgc
     7201 tcactttact gaacataaat cacagggcgt agttgtgctc tggaatgaga ataagcagca
     7261 aggatttacc aataatctta aacgggcgaa ccaagcattt ttacccgcat ctacctttaa
     7321 aattcccaat aagcgatcgc cctcgatttg ggcgtggtta aggatgaaca ccaagtcttt
     7381 aagtgggatg gacagacgcg tgatatcgcc gcttggaatc gtgaccatga cttaattacc
     7441 gcgatgaagt actcagttgt gcctgtttat caagaatttg cccgccaaat tggtgaggca
     7501 cgtatgagta aaatgctgca cgccttcgat tatggcaatg aggatatctc gggcaatgta
     7561 gacagttttt ggctcgatgg tggtattcgc atttcggcta cccagcaaat cgctttttta
     7621 cgcaagctgt atcacaacaa gctgcacgtt tctgagcgta gtcagcgcat cgtgaaacaa
     7681 gccatgctga ccgaagccaa tggcgactat attattcggg ctaaaacggg atactcgact
     7741 agtatcgaac ctaagattgg ctggtgggtt ggttgggttg aacttgatga taatgtgtgg
     7801 ttttgcgatg aatatggata tgcccacatc ggatggttta gggctgcgcc aagccatcac
     7861 aaaaaagtgc tcaaacagga gaaaattatt ccctagaagt ggttagcgcg tatttgtgtg
     7921 aaatagccgt catataagct gtaaagatat atggacaaaa tgctgatgct cgatgcgctt
     7981 gtagttaatg gctcatactt aatcggtcag gcctaatcga cttaagctcc gcgttaactt
     8041 tcagcctgag cattagcatc acattcctaa acgtaaaagc ctagccaatt tgctaggctt
     8101 ttttttatct caatggccat ggtcactcaa catgggggtg tgaataaagg agtggcgatg
     8161 tcagtaaagg tgaatgccgc tgcgataaag tgcctagccc atcaaggatt gtaggctttc
     8221 ttggctgcta aagcgtttga cgaaaaagtc aagaaggcgc tgatattggt tctccatcat
     8281 tttcatctac cggtttacta taggtcaacc aggccgtcct tgccggcgct tgggacgcag
     8341 acgatggcat caaatgcctc gctcaaattc gtttctacag aggcgctttg ggaccgcatt
     8401 cgctttgctt tcatgggggc atctgtcaat gtcagacatg aatcctccga tataggttga
     8461 tttttgatct cttcaacact tggtgttgtc cgactagaag acttatatgg atcagtattc
     8521 ttgcactgac aatggtgcca ctcaatccca ggatcgacct agttagaggc gtagatcaag
     8581 caattcaaaa cagcgcagaa gcagaaggag gtggaaatat gttccagggt gaaaggttac
     8641 atgcaactaa cgttgctctc taggatgtgc ctgctgtaac gtattgtcca gaaaagacaa
     8701 ccccccgtta tcgcttgcat cttggcggag ctgcgaaaaa cgggggccgt gacgaatcac
     8761 aagagaagtc tatcacatgg atcatgccaa acaaccccac gccgccaata agcgcggtgg
     8821 tgcttgagaa agtgcgcaca gcaggctgtt tcgggagcgc ctgccccgga aaccctacta
     8881 ttccgacgag cttacaacgg gcctacggat tgcggatgtt gcccgcgccc ttggtgcgcg
     8941 ttacatccag ccgaacgggc caacgcaccg ccactggatc gtttttgacg tagaccatgc
     9001 agccgctacg ttatcttggg atgacgtagg cgctccagcc cctaacatta ccgtcacgaa
     9061 caaggccaac ggccacgcgc atctgatcta cggactggat acgccgatcc gcactgcccc
     9121 ggatggaaat gcagcccctc tgcggtatgc cgccgccatc gaggccgcgc tccgggagaa
     9181 attaggggcg gatatgggct acgtccgggc tgatctgcaa gaaccccctg catgagcact
     9241 ggctggtgca ggtatgggaa ccccggctct acgatttagc ctggctatcc gattacctcg
     9301 atctgagctc ctacaatggc cgcaaatcgc ttcctgagta cgggcttggg cgaaactgta
     9361 ccctgttcga gaaaacgcgc ctgtgggcct ataaagcgat ccggcagggc tggccggatt
     9421 accccgaatg gttggccgct tgtgttgacc gggcatcagg atataacgcc cagttcgagc
     9481 agccgctccc gcaaacgaag tccggcacac ggccaagtcc attgccaaat ggacgcacca
     9541 gcacctaagc cccgcagggt tcagagagga gcaggcccgg agaggtgcga agggcggcaa
     9601 ggtatccaag ggagggggaa ggccgagcaa tagcggtaag gacaagtccg atctattgcc
     9661 ggaggttctg cggcttaagg ctcaggggta caccaaccgc gatatagcgg acgatcttca
     9721 aatctccccc agtacggtga gcgtttacct aaagcgtgat cgtccgtgat cgaaacaagc
     9781 cttatcagat aatagcccct ttagggggtt cctgcgctct tgtcttttag ccttaaaaat
     9841 ccttttagcg cggattggct acttgttggg ccgtgacggg gaaccgggaa ggcgcaacgc
     9901 tcgaccaggg caaccctgcg gtgattgaag ccctgattga ttcagtccct acgccaaaaa
     9961 tacacctctg gcgttctgtc gtttgccgag gctgatttac agggcgacca ggacaagctg
    10021 atgagcgact ttgaaaggcg ctcctgcccg gaactggagg gcaaccagta cgctgttttg
    10081 tgggtagagc accgcgacag g
//
