LOCUS       NZ_CP036326             3993 bp    DNA     linear   CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain VBA2172 plasmid p6_VBA2172.
ACCESSION   NZ_CP036326
VERSION     NZ_CP036326.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07572687
            Assembly: GCF_002300675.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Venkatesan,M. and
            Narasimman,V.
  TITLE     VBA2172 Klebsiella pneumoniae hybrid assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Venkatesan,M. and
            Narasimman,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2019) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 3993)
  AUTHORS   Shankar,C. and Veeraraghavan,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2017) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Canu v. 1.7; Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 69.57x
            Sequencing Technology :: IonTorrent; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 22:47:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,548
            CDSs (total)                      :: 5,423
            Genes (coding)                    :: 5,177
            CDSs (with protein)               :: 5,177
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 160 of 246
            Pseudo Genes (incomplete)         :: 93 of 246
            Pseudo Genes (internal stop)      :: 28 of 246
            Pseudo Genes (multiple problems)  :: 30 of 246
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036326.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="VBA2172"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p6_VBA2172"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2016"
                     /collected_by="Christian Medical College"
     gene            110..964
                     /locus_tag="CK244_RS28235"
                     /old_locus_tag="CK244_028235"
     CDS             110..964
                     /locus_tag="CK244_RS28235"
                     /old_locus_tag="CK244_028235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071579761.1"
                     /translation="MIIQEEHNIDFFKNQMPNKPYCTNNLDMGLSIRNKAKALEMLYLQ
                     ANQPAIQTCLLFDLDKKNSFYTFEQVGLPIPHFITKTPKTGRCHYGYMLKAGVCKTQQA
                     RLKPLKYAAAVEMAMAKKLKADLGFAGLITKNPLNDHWSPFWSGADLYDLDYLADFVDL
                     EKPQIQKKKSEAYGLGRNVNLFEDLRQYAYRTVLNFKKISTFEKFENELLLKAQGLNTF
                     CNPQNPLQYKEIKATVRSVARWTWKNFDSHTFSQIQSNRAKKPRKTNVKNEMLDILMSK
                     GVL"
                     /name="replication initiation protein"
     gene            999..1223
                     /locus_tag="CK244_RS28240"
                     /old_locus_tag="CK244_028240"
     CDS             999..1223
                     /locus_tag="CK244_RS28240"
                     /old_locus_tag="CK244_028240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="WP_131082312.1"
                     /translation="MEMTGRSERTVRKYFSQSRDDYEKTAMDRRRQAYELRSQGLKWQQ
                     VADKMGCSYHGAVALYRRYVALDMPQNSL"
                     /name="plasmid replication protein"
     gene            complement(1458..1742)
                     /locus_tag="CK244_RS28245"
                     /old_locus_tag="CK244_028245"
     CDS             complement(1458..1742)
                     /locus_tag="CK244_RS28245"
                     /old_locus_tag="CK244_028245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_071579762.1"
                     /translation="MSKTTAQQIADLQNKLQALRTKKARQDRREETRQKIILGAEVAKV
                     LGKKPEEINKALILGLLSKINELDEEEKQEFTRLGLNILEEWKNENRKP"
                     /name="conjugal transfer protein TraD"
     gene            complement(1746..2129)
                     /locus_tag="CK244_RS28250"
                     /old_locus_tag="CK244_028250"
     CDS             complement(1746..2129)
                     /locus_tag="CK244_RS28250"
                     /old_locus_tag="CK244_028250"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071579763.1"
                     /translation="MNKLITEAEGAKIESVFKYILEASESILIEHGHVITGQIIKNKKF
                     FADNGHKDFERLEYYLSSAISSLVEDKVTEGIKDINTDENLVFKKINELSDKEKLILAE
                     CIIKHFEYSNVRGMQKAFNIFGG"
                     /name="hypothetical protein"
     gene            2373..3566
                     /gene="mobQ"
                     /locus_tag="CK244_RS28255"
                     /old_locus_tag="CK244_028255"
     CDS             2373..3566
                     /gene="mobQ"
                     /locus_tag="CK244_RS28255"
                     /old_locus_tag="CK244_028255"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_076997031.1"
                     /translation="MATFHISFKKLRRSEGKSSVYLSAYQNREKTKDNRTGATWDYSKK
                     EGFFGSAILSPAGTPAELVKDSGTLWNAVEAGEKRKDAELCRYLDIAIPKELDDGQKKQ
                     IVLDYCQENFVDYGMIADIAFHDLDSHNPHAHVMLTLRTVSPNGFGNKQREWNNRENIE
                     AWRVNWEIIANDRLKKYGHKSRIDSRSLKDQKEEAERKAVFAKTPKEKAKWISKTIELD
                     RTPMTRIHRHQWRKGQKLRAMEQKEKALLLKIANATYLQQTKPPQPEETMKIEKKPLAR
                     SIIDNLKALYKKTQHIFKSKPNVNKNKKEEKEDPQNFVSSYVINEFGEHILKTELETKP
                     KTGTGTGTGGNNGGGKMGSGSGMSPMNQPQPKIEEPEPEPMPEFEDQQPKRKKKFGM"
                     /name="mobQ"
     Typing          2374..3383
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="85.0"
                     /evalue="0.0"
ORIGIN
        1 agaactggta caggattgaa aaatctataa aaataaaaac cccaccctct gcgattttgt
       61 tttggcgaca gaaaacagag agtagggcca gataaaaagg actgaaaaaa tgataataca
      121 agaagaacat aacatcgatt tttttaaaaa tcaaatgcct aacaagcctt attgtacaaa
      181 taacctcgat atgggattaa gcataaggaa caaggcaaaa gcccttgaaa tgctgtattt
      241 acaggcaaat cagccagcca ttcaaacttg cttactcttt gatctagata agaaaaattc
      301 attctataca tttgagcagg tgggcttacc catacctcac tttattacaa aaacaccaaa
      361 aacaggtcgt tgtcactacg gctatatgct gaaagccggg gtttgtaaga cacaacaagc
      421 aaggttaaag ccgttgaagt atgccgcagc cgttgaaatg gctatggcga aaaaactaaa
      481 agcagatctg gggtttgctg gattgattac aaaaaaccca ctcaatgatc actggtcgcc
      541 gttctggtca ggtgctgacc tttatgacct ggattattta gctgattttg tggacttaga
      601 aaagcctcaa atacaaaaga aaaaaagcga ggcttatggt ttaggtcgca atgttaattt
      661 gtttgaagat ctacggcaat atgcttacag aacggtttta aatttcaaaa aaatatccac
      721 tttcgagaaa ttcgaaaacg aactactatt aaaggcacag ggcttaaaca ctttttgcaa
      781 tccacaaaac ccgttacaat acaaagagat aaaagcaaca gttagaagtg tagcccgttg
      841 gacttggaaa aattttgata gccatacatt ttcacaaatc caatcaaaca gagccaaaaa
      901 acctcgaaaa accaatgtta aaaatgaaat gttagacata ttaatgagca agggggtttt
      961 atgatcatta atggtaaaaa ataaaagcaa aagaaataat ggaaatgaca ggtcgtagtg
     1021 aaagaactgt aagaaaatat ttttctcaat ctcgtgacga ttacgaaaaa acagcaatgg
     1081 atcgtcgtcg tcaggcttac gaactacgaa gccaggggct gaaatggcaa caagtagccg
     1141 acaaaatggg ctgttcgtat cacggagccg ttgccctgta tcgccgttat gttgccttag
     1201 atatgcctca aaacagcctg taacgacttc taagcagttt ttccccctaa cctaaccaaa
     1261 ccctccaccc ctggggattt ttcccctttc cccgtatccg ggccctgtat ctcgaactga
     1321 gaggcactca ggtacccaac aatcaacaaa cccacccata agccctgaga gcctctctga
     1381 gcctttttaa gacctccagg caagcctggc aacccttcac agtaaaaaac cagccaagat
     1441 cggctggttt ttattgttca cggtttacga ttttcattct tccactcttc taaaatattt
     1501 aatcctaatc gtgtgaattc ttgtttttct tcttcgtcaa gttcatttat tttacttaac
     1561 aacccaagaa ttaaagcctt gttaatttcc tccggttttt tacctaaaac cttagccact
     1621 tctgcaccca ggattatttt ttgtcttgtt tcttcccttc tgtcctgtct tgcttttttt
     1681 gttcttaatg cttgtagttt attttgcaaa tctgcgatct gctgtgctgt cgttttactc
     1741 atccattacc ccccaaaaat attaaatgcc ttttgcattc ctctaacatt tgaatattca
     1801 aaatgtttaa taatgcactc tgctaaaatt aatttttcct tgtcactcaa ttcatttatt
     1861 tttttaaaaa caaggttctc atccgtgtta atgtctttaa tgccttctgt taccttatcc
     1921 tcgacaagag aactaattgc cgagctaagg taatactcta acctttcaaa atctttatgc
     1981 ccgttatcag caaagaactt tttgtttttt attatttgcc ccgtaataac atgcccatgc
     2041 tctattaata tgctttcaga tgcctctaat atgtatttaa aaacactttc gatttttgca
     2101 ccttctgcct ctgttataag tttattcata ataacctcct aattatttat tttgttattt
     2161 attacctcca ttatttactt tagtcaaata tattttagaa taaaacaaat aatatttaat
     2221 attgggcgaa gcctggggtt cattacttat tattttgagg tgcaaccgaa aaaataatat
     2281 tagtaatgcc cacttacacg tcgcttgcga cgtatgggcc ctgcggggct aaaaaccaaa
     2341 attcaaaaga caaaaaaagt aaggggtaga ctatggcgac atttcatatc agctttaaaa
     2401 aattacgaag atcagaaggt aaatcttcgg tttatttatc tgcatatcaa aaccgagaaa
     2461 agacaaaaga taatcgaacc ggggcaacct gggattattc aaaaaaagag ggatttttcg
     2521 gctctgctat cctctcccct gccggcacac ctgccgaact ggtgaaggac tcaggcaccc
     2581 tctggaatgc cgtagaggct ggcgagaagc gaaaggatgc cgaattatgc cgttatctgg
     2641 acatagccat ccccaaggag ctggacgacg gccagaagaa gcagatcgtc ctcgattact
     2701 gccaagaaaa tttcgtggat tatggaatga ttgcagacat agcatttcat gatcttgata
     2761 gtcataaccc tcatgctcat gtaatgctaa ctttaagaac tgttagccca aatggatttg
     2821 ggaacaaaca aagagaatgg aataatagag aaaacattga agcctggcga gttaactggg
     2881 aaataatagc aaatgaccga ttaaaaaaat atggtcataa atctcgtatt gactcccgtt
     2941 cattaaaaga ccaaaaagaa gaggcagaaa gaaaggctgt ttttgctaaa actccgaaag
     3001 agaaagccaa gtggatttca aaaactattg aactggacag aacgccaatg accaggatac
     3061 acaggcacca atggcgaaaa ggtcaaaaat taagagctat ggagcaaaaa gaaaaagcat
     3121 tattattaaa aatagctaat gcaacatact tacagcaaac caaaccacct cagccagagg
     3181 aaactatgaa aatagagaaa aagcctcttg caaggtcaat cattgataat ttaaaggcat
     3241 tatacaaaaa gacccagcat atatttaaat caaaacctaa tgtaaataaa aataaaaaag
     3301 aagaaaaaga agatcctcaa aatttcgtta gtagttatgt aataaatgaa tttggcgaac
     3361 atatattaaa gacagagtta gaaacaaaac ctaaaacagg cacaggaacc ggaaccggtg
     3421 gaaataatgg aggcggaaaa atgggttcag gctctggaat gtctcctatg aaccaaccac
     3481 aaccaaaaat tgaagagcct gagccggaac caatgccgga attcgaagat cagcaaccta
     3541 agaggaagaa gaaatttgga atgtagtata tagtggtata tattcctata tagtagtatc
     3601 acttaataca acttaatatt atttaatact atactataag atactacatt tttagagttg
     3661 tgatcctcgc cggttctatt tcccctttat gtattgccac agcttcatta actgacttca
     3721 taagcatttt aaaaaattta ctatgcttat ttcttttccg ttttttctgc actttctttt
     3781 ttttcatata ttcaccaaaa aaaatcgaat cgccaatttt tcttcgaaca atactctttt
     3841 ttcctttttt attgtttttc tatcttcggt aatgcttcgc accatagatc tgatagtaat
     3901 gctttgctgc cgtatgtaaa gaactgcccc aacttcgttg ggctgttatc tgatatggtt
     3961 agacgcacac ttgacaaatt tttgagatcg gtt
//
