LOCUS       NZ_CP036309             5596 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain WCHKP020098 plasmid p3_020098, complete
            sequence.
ACCESSION   NZ_CP036309
VERSION     NZ_CP036309.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08624622
            Assembly: GCF_003037005.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Liu,L., Liu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP020098
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Liu,L., Liu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 5596)
  AUTHORS   Liu,L., Lin,J., Tang,G., Yang,B., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 22:43:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,874
            CDSs (total)                      :: 5,754
            Genes (coding)                    :: 5,588
            CDSs (with protein)               :: 5,588
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 58 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 20 of 166
            Pseudo Genes (multiple problems)  :: 23 of 166
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036309.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP020098"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3_020098"
                     /geo_loc_name="China: Chengdu, Sichuan"
     gene            complement(join(5403..5596,1..52))
                     /locus_tag="C6M05_RS31915"
     CDS             complement(join(5403..5596,1..52))
                     /locus_tag="C6M05_RS31915"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     gene            303..812
                     /locus_tag="C6M05_RS31350"
     CDS             303..812
                     /locus_tag="C6M05_RS31350"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            819..1031
                     /locus_tag="C6M05_RS31355"
     CDS             819..1031
                     /locus_tag="C6M05_RS31355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1698..1922
                     /locus_tag="C6M05_RS30010"
                     /old_locus_tag="C6M05_30000"
     CDS             1698..1922
                     /locus_tag="C6M05_RS30010"
                     /old_locus_tag="C6M05_30000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1906..2172
                     /locus_tag="C6M05_RS30015"
                     /old_locus_tag="C6M05_30005"
     CDS             1906..2172
                     /locus_tag="C6M05_RS30015"
                     /old_locus_tag="C6M05_30005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2242..2592)
                     /locus_tag="C6M05_RS30020"
                     /old_locus_tag="C6M05_30010"
     CDS             complement(2242..2592)
                     /locus_tag="C6M05_RS30020"
                     /old_locus_tag="C6M05_30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2658..3203)
                     /locus_tag="C6M05_RS30025"
                     /old_locus_tag="C6M05_30015"
     CDS             complement(2658..3203)
                     /locus_tag="C6M05_RS30025"
                     /old_locus_tag="C6M05_30015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(3229..3555)
                     /locus_tag="C6M05_RS30030"
                     /old_locus_tag="C6M05_30020"
     CDS             complement(3229..3555)
                     /locus_tag="C6M05_RS30030"
                     /old_locus_tag="C6M05_30020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(3913..4071)
                     /locus_tag="C6M05_RS32105"
     CDS             complement(3913..4071)
                     /locus_tag="C6M05_RS32105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4711..4887
                     /locus_tag="C6M05_RS31360"
     CDS             4711..4887
                     /locus_tag="C6M05_RS31360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4877..5221
                     /locus_tag="C6M05_RS30040"
                     /old_locus_tag="C6M05_30030"
     CDS             4877..5221
                     /locus_tag="C6M05_RS30040"
                     /old_locus_tag="C6M05_30030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          4546..4625
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3963..4360)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          3..1147
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.738"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5212..5594
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="3.78e-76"
ORIGIN
        1 tccgcgcatc gatgcgtggc agaccgtggt gaacgggcga ctggggctgc acgacccgaa
       61 cgcaccggag aaccggcgcg cgctggtgac gccatcttcg ttacccaaaa cgaagcagga
      121 agccgcagag gctattacgc ggggcttact ggccctcgcc tcgtcagggg agcttaaaac
      181 gcgtcaggac gtcactgagg cgctggaaag cgcaggtttt gaggtcgtgc gcaccacaaa
      241 gagcagcatc agcattgccg acccggacgg agggcgaaac atccgactta agggagccat
      301 ctatgaacag tcttttaacg ctggcgaagg acttagagca gaaatcgaaa gcgcagcagc
      361 agagtaccgg cgaaatgctg aaagccgcat tcagcgagca cgagaagtct gtcagagcgg
      421 aactgagcga aagcgggaag agaatcagcg ccgccatccg cgcccacgag cagggtatga
      481 gcgcggccat gcagtcgaac cgcctgagcg tgatgcgtat ggtcgggcgg acatggctga
      541 ccatcacgat ggtgtcagga ctgctgttcg ccagcctgag cggggtgttg tggtatcagg
      601 ggagcttgat agcgtcaaat ctggccgaaa tcgaccggca gaacgcagcg ctgtcgaagc
      661 tgaacgcgaa gacctggggc gtgacgtacc tggaagacag caacggacgt tttctggtgc
      721 tgccgaaggg gacggccgta gacaggacgc agagctggac ggtcgggaac gggcggtcga
      781 agcagaacgc gctgagactg gtgaaggagt aagtgccgat gaccgagctg gaaaaacagt
      841 tgctgagcgc attagagcag ctacagcagg actactcgaa aaggctggac gagtgggaga
      901 gcgccttcgg ggaatggcgg aaaatgtcag ggcttatgca acgggagaac gcggcgctga
      961 gcgagcgcgt gacgcgcttg agtcagcagg tggaacgctt gagcgggcaa ctgcagcgtt
     1021 tgagccggta gtgcagcgtc atgagatggc cgtggccgca gaacgggcgc acgagcaacg
     1081 gcagcacgaa aaggaactga cggaagcgcg ttcccggcag cgaagctatg acggtccgtc
     1141 gctgggctga aatgcaggaa aacagggttc tcatcgggca tttacgctga aaccggatgt
     1201 cattcacctg gctgcatggc tatgcagcca ggtaaaattt atctgtcgcg aacggggcgg
     1261 tttatcgggt ggtttgttgc cggttttacc tgactgccac cccgttcgcg gcgaacccgt
     1321 ccggggcggt gcgggcaacg ggtgttatgt ttaatcagcg tgaacggcag atacaaaaaa
     1381 gccccggagg gcttttaact actgacttat ttttttgaag atttgctggg tttcaatttg
     1441 tcagtatttc ccggcgtttt tttattgtca cgctggcgtt tatcaggttc ccctgttgaa
     1501 tcggcaattc gtttcggacc cggcttaaga cccataatcg ttatcctttc aatagttaag
     1561 aggaaagccc tcaagctaga tatattgacc gcgttacgga ttatcaagag gggctaacaa
     1621 gattattttt tgttctttta ggttgattgt ggtactgttg atatacatgt atagcaaatg
     1681 gtgttcggga ggtttatatg cttgctatcc ggttatctga tgagattgag tcccgtctgg
     1741 actcgctggc gaagcaaacc ggcagaacaa agacgtttta tgcgcgggaa gcaatactgg
     1801 cgcatctgga agacctggag gattattatc tttcagcaga aactgctgca cgcgttcgcc
     1861 gtggtgatga agcagtgcat tcgtctgaag acgtgaggaa gtcacttggt ctggacgatt
     1921 aactattccg atcgggcgct caaatcgtta cgcaagatgg acaaacagaa cgcacgacgg
     1981 attgtggatt ttatgagttt acgcattgca gttgctgccg atcctcgcca gtcagggaag
     2041 ccgctcaaag gtgagctggg cgagttctgg cgctatcggg tgggagatta tcgcgttctg
     2101 tgtgagatcc gagatgacga gcttgttatc cttgccgcca cgattggaca tcgccgcgaa
     2161 gtttacgact gaagcccgct atgcgggctt ttttgatgcc gacaacaggt attatggtaa
     2221 atactcgact aactacgtgt tctactgttt gtcggtttta gttaaccagc aggttgtctt
     2281 aacgataatg tagccgttat ttttaactaa taaggtcata ttgttaacgt caggcataat
     2341 aaaagacatt atggcctcag agttatcatc agaagcagct ctctgcatta ctacttcgat
     2401 tcccttttct ttgaatctat aagtatccag ttgcctatta aacttagaaa gacctacaac
     2461 catacttagt ttgttatctt taacagtaag agatgcttcc tgagtaccat tacatcggta
     2521 gtaagcggtg taaccgtcag caaaagatcg accggggaat ataaatgcta ggaacgcaat
     2581 taaccaatac atagttgaga atcttggtat tacacatttc actatcctct ctttcatttt
     2641 tcgttcacca tactttgtta tctgattttt aatttttcta ttttataaac caagtctcta
     2701 ccaaaaaggc gaaaattatc ctcaaataat aataattgtt taagaacatc catgaaagca
     2761 gctgcttcac cctcagttat gggagctact ctatataaca tataacctga gttaagggtt
     2821 tcttttatac tttcttcaat gctcattata aatattagtg gattatcatt tttcttcccc
     2881 catgggtttc taattaatcc ttcgatgtac tcaggtcctt tgcgtcctat tgcctgcgca
     2941 atatttttta tttcatctgc gtgagggctt tgttgtacag ctttgtttat gcctgcttga
     3001 gtgattttca gagactctac accgtgtatg ttgtatgaga taaaatgtaa taaattaaga
     3061 tgcaatgcaa ttaatccatt tttgtttaca tctgagtatt tggataaaaa acgtaatgaa
     3121 gtgttaactc tcccttgaag cctaacaatt gggagtgctg ccttgtatat ttccttctcc
     3181 gttaaatacc gtttaaaaaa cataattctc cgcctccgta tagcgtgcct attttatttc
     3241 gtcgtaaaac ttagctctga aatattcctt tagaaccatc atgtcattaa taattgaatc
     3301 tatctcatcc attttttctt ggtattcttt taatggtgaa gggagcggta actcaatatt
     3361 tgaaaagtga ttggcgagat agggatcaag gtttctcatc cattcaggct tttcgccaat
     3421 gcgttcttca actgaaagtg aacctgataa tctctggtta ataagatcac ttagactcat
     3481 tttggtttta ttcgaaagcc gttgtaagga ttgctttaat cctatatcta gcatgatatt
     3541 tgttctttct ctcattaccc ccccttgcta ttgcgttttt tgttgcgcac aaagctacca
     3601 ttgcgcatta agaaacgcaa taaaaaagga gggagggagt ggtttgtaca tgatgtgcag
     3661 tgcgctgagg gcatcagtgg cgggtgtttt tccgtgggat ggatggagct ccggggataa
     3721 cagaactgac gatcatttaa aggatcttct tgagatcctt tttttctgcg cgtaatctct
     3781 tgcactgtaa acgaaaaaac cgcctgggga ggcggtttga tcgaaggtta agtcagttgg
     3841 ggaactgctt aaccgggtaa ctgggtatac gagaccgcag ataccaaaac tgttctttca
     3901 gtgtagccgc agttaggcac ccacttcaag acctctcaat atctctctcg tatatatcca
     3961 gttaccaatg gctgctgcca gtggcgtttt tgcgtgtccg tccgggttgg actcaagatg
     4021 atagttaccg gaaagggcgc agcagtcggg ctgaacgggg ggttcgtgca tacagcccag
     4081 cttggagcga acgacctaaa ccgagccgag ataccagcag cgtgagctat gagaaagcgc
     4141 cacgcttccc gaaagggaaa aaggcggaac aggtatccgg taaacggcag ggtcggaaca
     4201 ggagagcgca cgagggagcc gccggagaga aatcaccggc atctttaagt cctgtcgggt
     4261 ttcgtcctcc tctgacttga gcgtccattt ctgtgatgct cgtcagggga ggcggagcct
     4321 atggaaaaac ggcaacgccg catccttttc tccggccctt tctgatttga gcgcctttgc
     4381 tttatcccgt tatccgtgag tcggtcacgc ccgccgcagc cgaacggcag gagcgcagct
     4441 accgagtgag cgaggaggcg tcatttcatc tgtccgtgca tggtattgcg gacgctcctg
     4501 agccacatat cacacctgcc acatgaggca ctttcatgaa tcgtgctctc atgccaacat
     4561 agtaagccag tatacacagc gtaaacgctg tgactggttc agggctgcgc cccgaaaccc
     4621 gctaacacca ctgacgcgct ggcgcttgtc caacgccgga gcgacccgga aaacttttcc
     4681 gtgccgtccg gcgtttgcga aggagggata atgcagaagc cgaagaagaa gccgctcgtc
     4741 gtaaaaaaaa gatcctggca tccggcagtg atggcaaatc tgatgcgtca ggcacaggag
     4801 gaagattacc gccgggagca gatggcagag cttgagcgtt cagcccggtc acattttgcg
     4861 gatatcggag gagaggatgg cggttaagcg tacccgtttt ttaggtatcc gggtgaccga
     4921 tggggagtac cagcagctgc tggagcgctg caatggaagg caactggcgg tgtggatgcg
     4981 ggaaacctgt ctcgatacgc gcccggcgcg gtcgttgcgg ctgccgtcca tcgatccggt
     5041 tttgctgcgc cagcttgccg ggatggggaa caacctcaac cagatcgccc ggaaaattaa
     5101 cggcggccag tggtcaggcg ctgaccgggt tcaggtggtg gccgcgctga tggccattga
     5161 tgccgaactc gagcggctgc ggcatgccgt gctggaaaag ggggcagacg atgatcgtta
     5221 agtttcatcc gcgagggcga ggcggcggtg ccgggccggt ggattatctg ctggggaaag
     5281 accgccagcg cgacggggcc atcgtcctgc agggaaagcc ggaggaggtc cgggagctta
     5341 tcgacgcctc gccctacgcc aaaaagtaca cctccggcgt cctgtctttt gctgaacagg
     5401 atttaccgcc cggccagcgt gaaaagctga tggcgagctt cgagcgggtt ctgatgcccg
     5461 gactcgataa agaccagtac agcgtgctgt gggttgaaca ccgggacaag gggcggctgg
     5521 aactgaattt tctggtaccg aacacggaac tgctgaccgg caggcgtctc cagccgtact
     5581 acgaccgggc agaccg
//
