LOCUS       NZ_CP036308            11970 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain WCHKP020098 plasmid p2_020098, complete
            sequence.
ACCESSION   NZ_CP036308
VERSION     NZ_CP036308.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08624622
            Assembly: GCF_003037005.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Liu,L., Liu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP020098
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Liu,L., Liu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 11970)
  AUTHORS   Liu,L., Lin,J., Tang,G., Yang,B., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 22:43:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,874
            CDSs (total)                      :: 5,754
            Genes (coding)                    :: 5,588
            CDSs (with protein)               :: 5,588
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 58 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 20 of 166
            Pseudo Genes (multiple problems)  :: 23 of 166
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036308.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP020098"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_020098"
                     /geo_loc_name="China: Chengdu, Sichuan"
     gene            join(11027..11970,1..742)
                     /locus_tag="C6M05_RS29925"
                     /old_locus_tag="C6M05_29915"
     CDS             join(11027..11970,1..742)
                     /locus_tag="C6M05_RS29925"
                     /old_locus_tag="C6M05_29915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            752..1009
                     /locus_tag="C6M05_RS29930"
                     /old_locus_tag="C6M05_29920"
     CDS             752..1009
                     /locus_tag="C6M05_RS29930"
                     /old_locus_tag="C6M05_29920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            1094..1243
                     /locus_tag="C6M05_RS29935"
                     /old_locus_tag="C6M05_29925"
     CDS             1094..1243
                     /locus_tag="C6M05_RS29935"
                     /old_locus_tag="C6M05_29925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            2727..4691
                     /locus_tag="C6M05_RS29945"
                     /old_locus_tag="C6M05_29935"
     CDS             2727..4691
                     /locus_tag="C6M05_RS29945"
                     /old_locus_tag="C6M05_29935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4691..5422
                     /gene="mobC"
                     /locus_tag="C6M05_RS29950"
                     /old_locus_tag="C6M05_29940"
     CDS             4691..5422
                     /gene="mobC"
                     /locus_tag="C6M05_RS29950"
                     /old_locus_tag="C6M05_29940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            5429..5959
                     /locus_tag="C6M05_RS29955"
                     /old_locus_tag="C6M05_29945"
     CDS             5429..5959
                     /locus_tag="C6M05_RS29955"
                     /old_locus_tag="C6M05_29945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5986..6165)
                     /locus_tag="C6M05_RS29960"
                     /old_locus_tag="C6M05_29950"
     CDS             complement(5986..6165)
                     /locus_tag="C6M05_RS29960"
                     /old_locus_tag="C6M05_29950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6191..6619)
                     /locus_tag="C6M05_RS29965"
                     /old_locus_tag="C6M05_29955"
     CDS             complement(6191..6619)
                     /locus_tag="C6M05_RS29965"
                     /old_locus_tag="C6M05_29955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(6783..7097)
                     /locus_tag="C6M05_RS29970"
                     /old_locus_tag="C6M05_29960"
     CDS             complement(6783..7097)
                     /locus_tag="C6M05_RS29970"
                     /old_locus_tag="C6M05_29960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7085..7363)
                     /locus_tag="C6M05_RS29975"
                     /old_locus_tag="C6M05_29965"
     CDS             complement(7085..7363)
                     /locus_tag="C6M05_RS29975"
                     /old_locus_tag="C6M05_29965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(7538..7903)
                     /locus_tag="C6M05_RS31910"
                     /old_locus_tag="C6M05_29970"
     CDS             complement(7538..7903)
                     /locus_tag="C6M05_RS31910"
                     /old_locus_tag="C6M05_29970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(7900..8271)
                     /gene="tolA"
                     /locus_tag="C6M05_RS29985"
                     /old_locus_tag="C6M05_29975"
     CDS             complement(7900..8271)
                     /gene="tolA"
                     /locus_tag="C6M05_RS29985"
                     /old_locus_tag="C6M05_29975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(8545..8790)
                     /locus_tag="C6M05_RS29990"
                     /old_locus_tag="C6M05_29980"
     CDS             complement(8545..8790)
                     /locus_tag="C6M05_RS29990"
                     /old_locus_tag="C6M05_29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            9435..10922
                     /gene="ltrA"
                     /locus_tag="C6M05_RS30000"
                     /old_locus_tag="C6M05_29990"
     CDS             9435..10922
                     /gene="ltrA"
                     /locus_tag="C6M05_RS30000"
                     /old_locus_tag="C6M05_29990"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     BGC             6027..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1347..1925)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4692..5419
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gcaggcatgg gacgccgctc atccggaaga ggggctgaaa agagactatg ataaagcgaa
       61 agctgagctg gatgccgaag ataaaaatat tgcgacctta aacagccgca ttgcatcgac
      121 agagaaggcg ctccccggtg caagggctgc tgttcaggaa gccgataaaa aggtaaaaga
      181 ggcagaggca aataaagatg attttgtgac ttataacccg cctcatgaat atggctccgg
      241 gtggcaggat caggttcgct atcttgataa ggatattcag aatcagaatg agaaattaaa
      301 agcggctcag gcatctttaa acgcaatgaa tgaatcctta tccagagata aggctgcact
      361 taccggggcg atggagagcc ggaaacaaaa ggagaaaaaa gcgaaggatg cagaaaataa
      421 gttaaatgag gaaaagaaca agcctcgcaa ggggactaaa gactacggcc atgattatca
      481 tccagtacct aaaacagaag acataaaggg acttggagag ttaaagcgag gtgatcctaa
      541 aacacctaaa caaggtggtg gtggtaagcg ggcccgatgg tatggcgata aaaaacgtaa
      601 aatttatgaa tgggattccc agcacggtga gcttgaaggg tatcgtgcca gtgatggcga
      661 acacctaggc gcatttgatc ccaaaacggg taagcaggtt aaagggccag atccaaaacg
      721 gaacattaaa aaatatcttt gagaggtaaa tatggggctt aaattacgtt tgacatggtt
      781 tgataagaaa actgaagagt ttaaagggga agagtattct aaagactttg gtgacgatgg
      841 ttctgtcatt gaaagcttgg gaatgccttt aaaggataat attaataatg gtgagtttga
      901 tttgaagaaa gaatgggttc ctttactaca gccatttttt aaaaatcaaa taaaacctga
      961 tgtggataat tgttatatag cttttgatta tcgggacggt aactggtaac aaaaaattat
     1021 caaggatgat ttgaaatact gacttccggt gttcagggat gaatgccgga gtctttaaat
     1081 acagggaaag tatatgaaaa aggtaaagac tatttttctt tttatattga ttgttgctgg
     1141 ctttcttctg gccgcatgtc aggcaaatta tatccgggat gttcagggcg gaacggtggc
     1201 accatcgtcc tcctctgaac tgacggggat cgcggttcag tagaaaagat caaaggatct
     1261 tcttgagatc ctttttttct gcgcgtaatc ttttgccctg taaacgaaaa aaccacctgg
     1321 ggaggtggtt tgatcgaagg ttaagtcagt tggggaactg cttaaccgtg gtaactggct
     1381 ttcgcagagc acagcaacca aatctgtcct tccagtgtag ccggactttg gcgcacactt
     1441 caagagcaac cgcgtgttta gctaaacaaa tcctctgcga actcccagtt accaatggct
     1501 gctgccagtg gcgttttgcg tgctttcccg ggttggactc aagtgaacag ttaccggata
     1561 aggcgcagca gtcgggctga acggggagtt cttgcttaca gcccagcttg gagcgaacga
     1621 cctacaccga gccgagatac cagtgtgtga gctatgagaa agcgccacac ttcccgcaag
     1681 ggagaacggc ggacaggtat ccggtaaacg gcagggtcgg aacaggagag cgcaagaggg
     1741 agcgacctac cggaaacggt ggggatcttt aagtcctgtc gggtttcgcc cgtactgtca
     1801 gattcatggt tgagcctcac ggctcccaca gatgcaccga aaaagcgtct gtttatgtga
     1861 actctggcag gagggcggag cctatggaaa aacgccaccg gcgcggccct gccgttttgc
     1921 ctcacatgtt agtcccctgc ttatccacgg aatctgtggg taactttgta tgtgtccgca
     1981 gcgcccgccg cagtctcacg cccggagcgt agcgaccgag tgagcgagga ggcgcaattt
     2041 ccccggcacc tgcaagacgg tcaccggacg cgctgatgtg ggatattgtc ccctgattaa
     2101 gtctttctat tccctgacga tcaattatca ggataggtga accatgtcgg tttttcgctg
     2161 aacattttac gttaacctat ctctgttgct tttttctcct tgcggagaaa tgcaagtcat
     2221 ccccgctggg gcgtggctga cttcagggga aaagaccttg cttacgctgg ctggacgcca
     2281 ccgacgcacg gtcttttccc ctctttctgg ctctgtccgg ctggacgccg gccacgccag
     2341 aaattcaccc ggcccccgtc aggctcacgc ctgaccaccc acaaaaatcc acggtgctgg
     2401 acgcacctga cgaagcagca aagctgctcc tgattttgcg ggtggtcagc cgctcgctat
     2461 ggcgaactct gacgggggcc ggaacaccgg aaaagaccgc actggggtgc cgccttttcc
     2521 tctcacacgc tggggcgtgc tcgttgttcc tccggggctg cgccttttcc gcatggggcg
     2581 gaaagaggct ggcctccgcg gtgaaagctg aacgcaatca ccacggacgt gaccgccgcc
     2641 gccatgcctt ggggcatgtc cgcggcttgt gcagcggggg ctgcgcatca cgccggtaaa
     2701 cccgtcgtct gaaggagaga gattgcatgt ttaatacgga ttcgcttgcc tggcagtggc
     2761 atatcccgga gctggcccgg cagatttacc cgtgggtcag ctggctgtac cgggagtatt
     2821 acagtctggc cggttttttt ctggttatcg gcctgttttt cggtctggct gaactctggg
     2881 ccggccgaat gcgttttgtg gggctgagcc ataaggcgcg gcgggagtat gccgatcgct
     2941 ataagcgcga taataaccgg actgtgccat tttcggtgct ggcctcgctg gattcacccg
     3001 ggcggatggt cagggcacgg cgacatgtct ggaactacgc gtactggctg accggcgctg
     3061 ccggtctggc ccttctgggg gggctggttg tcgccgtgtt tatcgagagc tggggagaaa
     3121 ccgtcctgcg cccggacctc tatgcgaacg cagattatta cctgctgcag ccggctaacc
     3181 tgttcgggat agcctggtgt gccggagggc ttgtcgccgg cgtccttctg gcgcggctgc
     3241 tggcgttcct gctgcttgat ggtcattttg cggccgctga cgaggcggtg aacgcgaaac
     3301 tgaatcagga gagccggcgc agcagccagc ggaccggaga aatgacagac gtccgccacc
     3361 tgcatttcgg ggagccggta ccggttaacg cgctggccga tttttccacg gagcaggccc
     3421 ggaaacagca ggcggtgttt ctgggtaagg atgagcaggg ccagccggtg ctggtccctc
     3481 gtgatacctg gcgcaagacc aatattcaga tcctcggcct gcccgggagc ggcaagagcg
     3541 cgatggccac caacgcgctc attcgctgcg tacgcgattt cggggacgct gtggtgtact
     3601 tcgaccccaa aggggacgcg tgggcgccgc acgtcttccg ggcgcactgc ccggacttca
     3661 ccctgctgga tctgcggccg gggaaaccgg cgcagctgaa tctgttccgc gatctcgacc
     3721 agtacgccct gaagaatctg ctggttgccg ggtttaacct gagtgagacc ggcgacgtgg
     3781 cggaccacta ccgcatcagc gagcagaaag cggcgaagct gattgcggag cagttcccgc
     3841 agggggcaaa cattcagcag gtgctggcgg cggcgtacgc gttgccggag gccctgaaaa
     3901 aggacgtgaa ggggctgatc accaagctgg agaacgtggc cgacctgagc gtcctgcaga
     3961 cggacagcgg tatcgatgtg gcggggataa tcaacggcgg cggctgcctg tacgtcatcg
     4021 gctcgatgga cgatgaggcg gtgatccggg tgcagaagat gctgtttgcc cgctgcgcgc
     4081 aaatcatcat tgcgcgggat gagttccgga ggtggccgca cgcgagcatc atgctcgatg
     4141 agattaaata tctgctgtcg aagtacgtgc tgaacgcgct ggggacgctg cgcagccggg
     4201 actgcaacct gctgctcgcg caccagtcgc tgggcgactt cggtcagtgc gggcaggatc
     4261 tgcccgctga ttttgtgaaa accacggtac tggataacac gccgatccgc tggttctatc
     4321 gcgcggccag ccaggagtcc gcgcagtggg cggcgggcca gaccggggag atccgggtgg
     4381 acgtggagcg ccgccgggcc agccgggagg cggggaacgt ggagcacatc agcggcgaca
     4441 gcttcatcca gaaggaggcg cggccgctgt ttgacgtgaa caccctgcaa cacctgcctg
     4501 acgggttcgc ggtgatgacc gggctgggcg tggcgcggct ggcgttcagc agcccgctgc
     4561 gggttgagcg ccgggaaatt tcgctgaaaa ccttcccggt gctggcgaaa acggacccgc
     4621 tggcggagta cctgcctccg gccgacggcg gcggaccaaa agcggatgat gattttgcgg
     4681 ggctttactg atgacactgg agcagtacaa cgtcagccac gcgaagcggc aggcgcggaa
     4741 cgcggagaaa acacggctga ccctgcgctg gctgcgggaa gagctgtgtt ccacggcgga
     4801 gctggttgcg cgacggctgg ggattgcggc ggtacagccg gtttaccgct tcctggacag
     4861 tctggtggcg aaagggctgc tggtccgggc gaaatacccg gttgacggcc gtcaggtcag
     4921 cgtgtggggg ctgacgccgc acggggtggc gttctcgttc gacgaggacg agccgctgac
     4981 ggacgttatt ccgtttcagc cgtcaagggt gtcggcggcg cagctgccgc accggctggc
     5041 cgtgcagtcc ctgcgcctct ccatggaggg ccggggggcc accggctggc ggtacctgca
     5101 ccgtatggcc ctgaaaggga tgaaagtgcc ggacgcgctg gcggagctgg acggcagaac
     5161 ggtggcattt gaggtagagc gcaccgtcaa gagccgcagg cggtatcagg aagtggtgtc
     5221 cgggtatctg ttcaaccgcc gggcgaacgg catcgatgaa atctggtaca tctgccctga
     5281 ccggccgacg cttgtccggg tacagcgggc gattctggcg gtggatgaaa tcgtgaatcc
     5341 acagaccggc gaggcccgca aaacggtgga gctggaccgg gagaggctgt tcgcgtgctt
     5401 taaatttatg acaacggagt gagggattat ggcgctgtac atggtattta ttgaggacgt
     5461 ggaagacaaa gacggcgagc gctgcattat gccaaccagc gagcatctgg aagacatggt
     5521 ggtgctcgcc tggcagaccg gggcgtatgt tttctgtttt gtcgaccggg cccagcatct
     5581 ggcgatgatg aaatccatcg ggatgtatga cgagcggctc aacagcgacg aaaaagccac
     5641 gtactttttc accggtgaaa atccgcaatg ggaacgggac aaacccacag gaatgaagct
     5701 gtgtgattac ctctatcgaa aggtggagcg gttcgccatc gagaacggtg tgcaggtggt
     5761 tgatgccggg aagtgctcac cggagcagat taacgcgatg attgaggtgg tcagcgagcg
     5821 gcacggggaa agcgcggtga acgagaagct gaaagcggag ctgatgcggg aatataacag
     5881 ccgctatttt aacgtgccgg tgcctgaact gccggaggac aaacccgccc gccggtggtg
     5941 gccgttcggc aaaaaataac gccagcaccg gcatggccgg tgctgttatt cttccccgag
     6001 cttatcccgc atcacctgcc agagtgtttc cgcatcctcg tgaagccgtt cgcacatgtc
     6061 ggccggggta tccatgtcca gctcgttcag cttttccagc aacagcagcg actgatcctt
     6121 aatgaattta gccatattga gggcagtttt ttcctgcttc gtcatgttct ccattccctt
     6181 cggtaagttc tcagctaaac cagccggacc agaagcgttt tttcttctgt gtcagttctg
     6241 ttgtcaatct gtccacttcg gcctggagtt tccggcgctc atcatcgtgc tggcggcgtt
     6301 cttcttcccg ggatgtttta tcttccagca tcagggtgac agcctgccgg agatcggcca
     6361 gttccttttg cattgccaga agctgttcct gtgggaaatt tttttgtgta ttttcacggt
     6421 gtgaaatgcc gggaatttgt ttctcaggga tattcacatt tccgtataca cggatcagtt
     6481 ctgacacatc cacgacaggg ttgtttttcc cgtcacgtga cacggtaacc tttccctgtt
     6541 ttacatgatt gtatagcgtt cttctggtaa tgcccgcggc ttgtgctgcc tgtgaaaggt
     6601 tgagcaatgt tttcgccact gtatacccct gattttatcg ggtgtgaaag gtgtgtaatt
     6661 tctcacggta aatgtatcgc tgaattttac ccgtttttcc gttcaggatg tggttttctg
     6721 acgcgctgcg cttgtcaaac cggcgacggc cacgcaatga attgcgcccc cgtcatcggt
     6781 tattagaggc tgtctgcccc cggatacccg gattctttct gatgcctgac ggtgacgatc
     6841 acaatctggt ccatttcccg gtcgtggcgg tagagcatca catagccact gtcgccaaac
     6901 ccgatcacca gttccttata ttccagcggc agaaacggca ccgggcggcc aatgtctggc
     6961 aatgttttca gttgctggat agcccggacg atcacctcac ctccttttct ggctgccagc
     7021 cgatttttgg ttttgagaaa gtcctgaagg cgttgtaaat cgcgctgtgc cagcgcggaa
     7081 atcgttacct gtggcatggc ggagcatcct gctcgttctc agttccccag gtacttatcc
     7141 aggcttccgc ttcttccgca gtaaggtgca gacccgtttc ctgatagtgc tgccatgcgg
     7201 cctgtccgtc ccgcaggtac tggctgcgct tctcttcccg gtcgatgtat tcggtgattg
     7261 cctccagcat tagcgcgtgc gctgagcggt gccggtcatc ggccagcgtt ttcagccggt
     7321 ccttaagttc ttcatcgagc ctgatggatg ttgctgatga cataaaagcc cctttgtagt
     7381 catatgtatt acacatgact actttaatcc gtggcggcgc tatttgccag aaatacggct
     7441 tttgctgccc atttttcagc ttcgcttcca ctaatcaggg ctgctacagg ctcactcatc
     7501 atccaggact attgcagcct gcgggttaaa ggacctgtta ctggcggcaa ccatcatcat
     7561 tgagcttttg ggtggtgact ggtcgcggat cctgtaagcc gtcatttttc agactgaatt
     7621 caaaatttat aacctgtcgc attgccgggt gccctgcggg atatagccag tgcctcattg
     7681 ccgtcagcgt ttcccgaaaa aaaaggtgac agccggtaag cgacacgttg tccactcgtc
     7741 catcgtccag aatatcaaac gtggcggtca cctgaccttc gatgcgtttt gctgcggcct
     7801 gtaccgggta ggttggctgc agtgtggtta cgggttttag tggtatcgca tgtaccggag
     7861 tgaggaacat ggccgccagc actgtcagag taaaagcttt catggtctga aatccagagg
     7921 ggcgtttttg aatatttgcc aggtattttc atccggtgcc ggcggaattt tggcgtgggt
     7981 aacggctgat attgctgctt tacagagttt cggatctcct ccttcagcaa tggcgctgtt
     8041 cagagtgcca tcacgttgta tggatatccg cacggtgcac atttttcccg cataggcctc
     8101 tggttcaaac atctgcattt caacagcggt cttgatggcc gcagcgtagc tactgatttt
     8161 agatgactta tccagagtat aaattttcgg cggctgggtg gtggaatgct tacaggccgt
     8221 caggagtaag gggctgcaca atgccagcag gataaagggt attaaacgca tcgggttgat
     8281 gacctgtcag taaaaaatgt tacatgtata tttgtcactc tttacggcca cgttcattta
     8341 cataaacacg gcttatcgta tcgtttttac accaaacggc ccgccatgca gttgcgtgca
     8401 taggcatgca gcgggttaca atttaccggg cgcgtttccg gcggttttcc gggtggtttg
     8461 ttgcctgttt tatcccgtag ccgccggaaa cgccctcaga ccgtctgagc ggtgcgggca
     8521 atgcgacgtt atggtaaata ccccttaccg gcggcgctgt ggctgccagg tgaaggtggt
     8581 ttccgtagcc tcgtatttct cccgacgcat ttcctgcgcc cgcctggctt cacgttcgtc
     8641 ctgaatggca cgaagcaccc tttcccgcac ctgttgtttt accgcctttg gcagcgtttt
     8701 gttccctttc aggcccatgg ccagtgcctg agcgacaaag gcgtccgcca ggcgcttttc
     8761 ttcagcggga gtcagtttca ggtgcgtcat agtggcctcc gggcgtctca ggtttcccga
     8821 acagtttaac cgcaggtcct ctcctcatgc cagttagctt tcagatgtgt ctataccatc
     8881 tgaaatgtaa gtgtgatgaa tgtcttaaat aataaaattt gttgccatga gaaattttcg
     8941 ggaaaagacg cgatctgctt acaggtggct ccgggcgctt ttttgtgcct gaagatgaga
     9001 actaatctca tatattgaca cctgaaaacg ggaggagtaa ggtaataatt atactgtgta
     9061 tatgtacagt gtgcgccggg agaccggtta gagatcaagg ggtgaaagtc cccgaccatt
     9121 gaaggaccag caatccacaa ggtccccgag tcatgcgttg cataccgcga ggtatggggc
     9181 gaagcgttga caggggtgtt gacaggccag ccattgagcc acgaaatgta tattaaatta
     9241 ccgggtgccg acgttgtact gttaacggaa ggcaacatca tagggtgcga tactgcgagt
     9301 gccacatgga cccggcgggg tctgagaccc tggcatgtca atacgatctc tacgcgggaa
     9361 ccgggagatc tcccctctga ccatctgcca gtgtcggaga tggcccgcac cgggaagacg
     9421 aggagtcata gccggtgatg tacggagagg agaagtcgga ctcgctcata gtagcggcga
     9481 agcaggcgaa caacccgaaa ggagcggagt cagtggagcg aaggagcggg gccaagggga
     9541 acgcggaaca gccacacatg cgccggacac agagccggga aagcatgtca cagaggctgt
     9601 cacgcgtgcg ggaagctgcg aagcagcgga agaaagaacg gtttacagca ttgttccacc
     9661 tgctgacagt cgaagcactg gaagccgcat tcctctccct gagcaggaaa gcggccgccg
     9721 gagtggatgg catcaggtgg atggactacg ccggaaacat gaagaacaac ataacagatc
     9781 tgcaccggag gctacatcag ggcagctaca gggcgcagcc cggcaggcgt cactacatcc
     9841 caaaagcgga tggaaaacaa cgcccgctcg gcatcgcctc gctggaggac aagatcgtcc
     9901 agtatgcgct ggtgaaaatc ctgaacgcag tctatgaaaa cgactttatg gggttctcat
     9961 acgggttcag acccgggcga agccagcacg atgcactgga cgcactggcc acagggctgg
    10021 tacgcactaa cgtaaactgg gtactggatg ccgacatcag tcagttcttc gacagggtga
    10081 gccacgaatg gctgatcagg ttcacagagc atcggatcgg cgaccggagg gtaatcaggc
    10141 tcatacgtaa gtggctcaca gccgggacgt cggaggaggg tcaatggcga gcaacggagg
    10201 aaggcacccc acagggtgcg gtcatctcac cgctgctggc aaacatatac ctccactacg
    10261 tcttcgatct gtgggcgcat cagtggcgac gtcgctatgc cacaggcaat gtggtaatgg
    10321 tcagatacgc cgatgacatc gtcatcgggt tcgacaaacg atacgatgcc cggcgcttcc
    10381 gtatagccat gcagcgcaga ctgagggagt tcggactcac ggttcacccg gagaaaaccc
    10441 gtctgatgga gttcggccgc ttcgctgccg aaaaccgtgc catcagggga aaaggcaaac
    10501 cagaaacgtt caacttcctc gggttcacgc acatcagcgg gaaagatcgc aacggcaggt
    10561 tcatgctgat acgaaagacc cgccgggatc ggatgacggc aactctgaaa gccatcaaag
    10621 acggtctgcg aaggcgctgg cattactcaa tccccgaaca gggaaaatgg ctcaggagag
    10681 tggttcaggg atacctgaac tatcactcgg taccgggcaa cttccccacc atgcagaagt
    10741 tcaggacaca cgtaacaaac ctctggcgcc gggcgctcag gcgcaggagc cagaaggatg
    10801 atacgacctg gacgaaagca aacaaactgg cagccgcatg gctaccaagg gttcgggttc
    10861 ttcatccatg gcctgtggag cggttcaccg ccagacaccc gaggcaggag cccggtgcgt
    10921 aaatcgcgca cgccgggatc tgtgcggggg gtatccggta acgggtatcc ctaccgcgac
    10981 atgttggctt taatttattg ttttaaatgt caaagaggaa aacggtatga gtggtggaga
    11041 cggtcgaggt ccgggtaatt caggtctggg acataatggt ggtcaggcca gtgggaatgt
    11101 gaacggtacg tctggtaaag gtggcccttc atcaggtggt ggtacggatc caaacagcgg
    11161 gccgggctgg ggtacgacgc atacgccgaa tggagatatt cataactata atccggggga
    11221 gtttggtaac ggcgggagta aacccggtgg taatggtggt aacagcggca atcatagcgg
    11281 tagctctggt ggcggacagt cttcggccac tgcgatggcc ttcggtctgc ctgccctggc
    11341 tactccgggg gctgaaggac tggctttatc agtttccggc gatgcgttgt cggccgccgt
    11401 tgctgatgtg ctggctgccc tgaaggggcc gtttaagttt ggtctgtggg ggattgcgat
    11461 ctacggtgtg ctgccttctg agattgcaaa agatgatccg aaaatgatgt caaaaattat
    11521 gacgtcatta ccggccgatg cggtgacgga gactccggca agtactttac cactggacca
    11581 ggcgacggtt cgtgtcagac aacgggttgt ggatgtggtg aaggatgagc ggcagcatat
    11641 tgcggttgtc gcaggtcggc caatgagtgt ccctgtggtg gatgcgaaac cgacaaaacg
    11701 tccgggggta ttcagtgtgt cgattccggg tctcccgtct ctgcaggtga gcgtaccgaa
    11761 aggtgttccg acagcgaaag ccccgccaaa aggcattgtt gctgaaaaag gtgattcacg
    11821 tccggctggt tttacagccg gtggtaactc ccgtgaggcc gttattcgtt tcccgaaaga
    11881 gaccggacag aagccggttt atgtgtcggt gacagatgtt cttaccccgg cacaggtaaa
    11941 acagcgtcag gaggaagaaa agcgtcgcca
//
