LOCUS       NZ_CP036207             4149 bp    DNA     circular CON 18-JUN-2024
DEFINITION  Escherichia coli strain L725 plasmid punnamed4, complete sequence.
ACCESSION   NZ_CP036207
VERSION     NZ_CP036207.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10219252
            Assembly: GCF_004301465.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Rao,Y.
  TITLE     Detection and characterization of Escherichia coli isolate carring
            NDM-enconding plasmid and MCR-carring plasimd
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Rao,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2019) State Key Laboratory for Diagnosis and
            Treatment of Infectious Disease, Zhejiang University, No. 79
            Qingchun Road, Hangzhou 310003, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.11.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004301465.1-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 05:40:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,000
            CDSs (total)                      :: 4,876
            Genes (coding)                    :: 4,716
            CDSs (with protein)               :: 4,716
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 53 of 160
            Pseudo Genes (incomplete)         :: 110 of 160
            Pseudo Genes (internal stop)      :: 25 of 160
            Pseudo Genes (multiple problems)  :: 27 of 160
            Pseudo Genes (short protein)      :: 1 of 160
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036207.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="L725"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="punnamed4"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="30.3 N 120.2 E"
                     /collection_date="2017-07-21"
                     /collected_by="Tao Lv"
     gene            complement(469..723)
                     /gene="mobC"
                     /locus_tag="D9K49_RS25165"
                     /old_locus_tag="D9K49_25165"
     CDS             complement(469..723)
                     /gene="mobC"
                     /locus_tag="D9K49_RS25165"
                     /old_locus_tag="D9K49_25165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            902..3049
                     /gene="mobQ"
                     /locus_tag="D9K49_RS25170"
                     /old_locus_tag="D9K49_25170"
     CDS             902..3049
                     /gene="mobQ"
                     /locus_tag="D9K49_RS25170"
                     /old_locus_tag="D9K49_25170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(3307..4008)
                     /locus_tag="D9K49_RS25175"
                     /old_locus_tag="D9K49_25175"
     CDS             complement(3307..4008)
                     /locus_tag="D9K49_RS25175"
                     /old_locus_tag="D9K49_25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(725..901)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          902..1260
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3308..4008)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.858"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          816..3046
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.989"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttttttttt gcaaccccct ttttctgtgg attacacccc caaaagctcc acctttcacc
       61 cccaaaagct ccacctttca cccccaaaag ctccaccttt cacccccaaa agctccactt
      121 atcttgccag aacatcttta aaatcatggt gttacaagac ctaaaaaggt tttaaaaaaa
      181 agatctaaaa ataagatctt aaaaacttaa cggcgagcct gtggataact tggggtgctg
      241 aaaacaaaaa accaataaaa acaatgcgtt gaaataaagc agaattgcaa gtttcgaata
      301 caccccctcc aaccctgtgg aaacccagta ttcatgcggg ttcagcgcat atcacctgcc
      361 gaaaaaccgt gcgtatgtgc tcaacctggc gactgattca cttccgcttc ctcgcccact
      421 cgctcgctaa cgctcggtcg ctggggctgc ggcgagcgga cgatacaatc actgttctgg
      481 cagcgcctcc agctcgttaa tcagcggcag tatgcgcgtc acatcagcct gtcgtgtcac
      541 ctctgccttc aggaactgga gtaaccaggc acgcaactgc acctcctccg gctttctgac
      601 ctttgccagc aatgcagcac ccacaacaac cttctgcgct gtgtccaggc tccggctctt
      661 cgccttcagg cgctgtaatc tcgcctcagc atcagcaatc ttctgttcga gcgttctgct
      721 catgatgtaa ctccgtgcgc gatgaaaaat cgcattttag cgcgtcactg gtagtttaaa
      781 aactgagctg gcataatgca cggcaaatca cgaagtgcgc acttatacaa tctccactcc
      841 gtttcgattg tgtgcgccct gcggggctga aagaaaacta caaaaaggca ttacggcaga
      901 aatggcgatt tatcatctca gcatgaaaat tatctccaga agcagcggct acagcgccgt
      961 agcttccgct gcctatcgtt ccggttcact gatgcttgat gagcgtaccg gactgactca
     1021 cgactacacc cgcaaaagcg gtgtggctga ggctgtgatt ctgacccccg ccaccgcccc
     1081 tgcctggtgt acgaaccgtg ccgaactctg gaatgccgtt gagaaggccg aacgccggaa
     1141 gaactcacaa cttgcgcggg aaattgaact cgccatcccc cacgaactgc cgcaggatgc
     1201 cgcacgggaa accgttctcg cctttgtcag agaaaacttt gtcagtcagg gcatgattgc
     1261 tgatgtggcg ttccatcaca tggataaaac aaatccccat gcgcacatca tgctgaccac
     1321 gagggctatc ggacctgctg ggttcagcgg aaaggtcagg gactggaacg accggacaca
     1381 tgccgagacg tggcgggcat catgggctga ccatgcaaac agggcactgg cgaacgccgg
     1441 ttatcaggaa gagatagacc atcgttcata cgagcgacag gggcttgaga aaacgcctgg
     1501 tattcacctc ggaaagtctg cctgtgcgat ggaaacacgc ggtatcgaaa cagaacgcgg
     1561 cgaacagaat cgtctgatta acaggcttaa cctggaaata cagatttccc gcaccgaact
     1621 caggaacgga gggctgacag agcctgcccg ccgtgtggct gaccttctga atatcgggct
     1681 tcctgacaac gcaacttcgt acacactacg tgacattatc gaagcgttgc cgaaggacag
     1741 taactcggca tggaaactca ccagtaatgc catgtcgatg atggcagata tgcaggcgac
     1801 gcgcagacgc tgggaagagc tgaacaccga acgaaaggaa gcgatgagcc atgctgccag
     1861 cctgaaaaaa tcatcgccgt tttcgtccgg tttttcccgt attcccctga tgcaatgggc
     1921 ggcaccggaa taccgtagtg agcagggaaa aatccagtct ctgagccagc aaatggaaaa
     1981 attacgacag cattaccgga aggtggagaa acaggacatt cccgcgagtc agatggcatt
     2041 tgaaaaacag tggcagcagt ggggggaatc cggccttgca gctctcagag cccagttgct
     2101 taaacgtgaa gaggaattgc gccggaagga acaggaagat actgaacgta gtcgcgcgga
     2161 gcagctcagg aaaaatgaca atgccgttat ggattcaggg gtgctgagag cagtcgttac
     2221 ggggtatggc gaggctccca tgcctggtaa cggggaaatg acctgttacc ttctgcttca
     2281 caaccggaac ggagaatata cgctctgggg gaacgagctg gagaaatacc gcaccagaat
     2341 ctttgagagt gttgatttaa tgcgtgacag gagtggttat atctgtgacc gaagcgaaat
     2401 cggccaacgt ccgccactgc aacgtgttta ttcctctgca acctttgagc aactgctgac
     2461 gcaggtttgc cagacatggc cgcaacatac ccgcaatctc cgccagccga agacatggcc
     2521 ggagtctttc tgtcttggag aagaccgcca gccagccatg ccatcaccag cggcaaggaa
     2581 ggtggatttt atgcagggac gtctgccacc gacgctgatg ccggtcatga gttctgtcga
     2641 ccgggaaacc cgccagcttc agcttttgct ggtcatgggc gtggatgact ctctgggcgg
     2701 tgttatccgg cttaatggta cgctgtatcc cgcgtttgcc gttccctcag ccgataacag
     2761 gcatctggtc atcagtgcgc tgaccgataa ggggttacgg tatgcagggt atggtgttgc
     2821 cgtaaatcac gatgcagaca gccacatatc acctgcacca gaactgatgc agtttcatct
     2881 gaaaacccgt gaggcaccgc tttttgctgc cgtcaacaca ccagaaaaac agccggatca
     2941 tctgttccgc tcgctgggtt ttaatcgtac ctgggaggaa tggcagcgcg aggaagatgc
     3001 acgaacacac accaccgaga ggagacatga tcgcggttgg tcacagtgaa tgtgaacaat
     3061 attttgtttt gtttaacatc gtgttatgtt cgtcgatgtt cttcgatgtt catcgacagg
     3121 gatgaatgca agaagcacac tcacataacg caagcatagt tttgtttttc cgcccccaaa
     3181 aattgggggg tcttttttcg ctgcatcaga gcgtttttga cgcaattttt cacttcaata
     3241 gtaccttcgc actactaaca caaaaaaaac gcagcaggga gcgttttagg tgccttaaaa
     3301 tcgattttag atcgtcatct tgatctgttt gtcttccttg aagcgaaaat gcagggctac
     3361 aaccgtgcgc ccctttttga tcgtctcaac agttacaacc agatcgcttt tttcgttcag
     3421 ttcggcgatg gctggcttga ttatcatctt attcaagtct ttgaattgtt gatatttatc
     3481 ttcaacggca agcatggttc ggaaatcatc gatagctatc gttctgtcac cggtgctgcg
     3541 gaactgctgg agcagctcgt aaatgcggat gctgtaggtg ctggaaaggc tggagacgtt
     3601 cttcacgact acccgcgtaa actgcccctt gagctgtgtc agatacggca tgatggcatc
     3661 ggagaaggtg atatctactt tcgcctcacc tttgaaatac tgcgcccggt actgcaccca
     3721 gcggaactct tctcgttttt cctcgttctt gatgattact gacctgtccc agaggcgatc
     3781 tatcgcttcc ttgagcctag cttcagcgtg ctgccgtccc atgtcaggaa aggcatcatg
     3841 aaactctgca gccgtgatgg tcatggtttt ttgttcatcc ggcgaagggg aatcttctcc
     3901 ggatttcagg cggctgatac agaccagcag gaagcgctgt tcctgcaaag tgagcttgta
     3961 gcttgcctcg ataagggcgt ttgatttgta agctatcaga tctgacattt cgcttgctcg
     4021 ttgctattgg tggcgttttt ttactgtacg ccacttatca tatgggtgga agtttttttt
     4081 ttgcaacccc ctttttctgt ggattacacc cccaaaagct ccacctttca cccccaaaag
     4141 ctccacctt
//
