LOCUS       NZ_CP036197             6140 bp    DNA     linear   CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain BA34918 plasmid pColKP3.
ACCESSION   NZ_CP036197
VERSION     NZ_CP036197.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10956502
            Assembly: GCF_004295405.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Narasimman,V. and
            Venkatesan,M.
  TITLE     BA34918 Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Narasimman,V. and
            Venkatesan,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2019) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method :: Canu v. 1.7; Unicycler hybrid v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 37.6x
            Sequencing Technology :: IonTorrent; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 23:31:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,183
            CDSs (total)                      :: 6,057
            Genes (coding)                    :: 5,729
            CDSs (with protein)               :: 5,729
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 328
            CDSs (without protein)            :: 328
            Pseudo Genes (ambiguous residues) :: 0 of 328
            Pseudo Genes (frameshifted)       :: 181 of 328
            Pseudo Genes (incomplete)         :: 155 of 328
            Pseudo Genes (internal stop)      :: 40 of 328
            Pseudo Genes (multiple problems)  :: 45 of 328
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036197.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BA34918"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pColKP3"
                     /geo_loc_name="India"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2017"
                     /collected_by="Christian Medical College, Vellore, India"
     gene            <1..775
                     /locus_tag="EX353_RS31410"
                     /old_locus_tag="EX353_31410"
     CDS             <1..775
                     /locus_tag="EX353_RS31410"
                     /old_locus_tag="EX353_31410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_130628036.1"
                     /translation="HAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGN
                     AAPLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLS
                     SYNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQP
                     LPANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLP
                     EVLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            1182..1574
                     /locus_tag="EX353_RS31415"
                     /old_locus_tag="EX353_31415"
     CDS             1182..1574
                     /locus_tag="EX353_RS31415"
                     /old_locus_tag="EX353_31415"
                     /inference="COORDINATES: protein motif:HMM:NF037437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            1556..1894
                     /locus_tag="EX353_RS31420"
                     /old_locus_tag="EX353_31420"
     CDS             1556..1894
                     /locus_tag="EX353_RS31420"
                     /old_locus_tag="EX353_31420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1875..3329
                     /locus_tag="EX353_RS33460"
     CDS             1875..3329
                     /locus_tag="EX353_RS33460"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3611..3892)
                     /locus_tag="EX353_RS31440"
                     /old_locus_tag="EX353_31440"
     CDS             complement(3611..3892)
                     /locus_tag="EX353_RS31440"
                     /old_locus_tag="EX353_31440"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            4236..5033
                     /locus_tag="EX353_RS31445"
                     /old_locus_tag="EX353_31445"
     CDS             4236..5033
                     /locus_tag="EX353_RS31445"
                     /old_locus_tag="EX353_31445"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            complement(5440..>5592)
                     /locus_tag="EX353_RS31450"
                     /old_locus_tag="EX353_31450"
                     /pseudo=""
     CDS             complement(5440..>5592)
                     /locus_tag="EX353_RS31450"
                     /old_locus_tag="EX353_31450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     ARG             4237..5030
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          7..762
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.462"
                     /coverage="97.0"
                     /evalue="8.52e-173"
     Typing          1876..3326
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccatgcagcc gctacgttat cttgggatga cgtaggcgct ccagccccta acattaccgt
       61 cacgaacaag gccaacggcc acgcgcatct gatctacgga ctggatacgc cgatccgcac
      121 tgccccggat ggaaatgcag cccctctgcg gtatgccgcc gccatcgagg ccgcgctccg
      181 ggagaaatta ggggcggata tgggctactc cgggctgatc tgcaagaacc ccctgcatga
      241 gcactggctg gtgcaggtat gggaaccccg gctctacgat ttagcctggc tatccgatta
      301 cctcgatctg agctcctaca atggccgcaa atcgcttcct gagtacgggc ttgggcgaaa
      361 ctgtaccctg ttcgagaaaa cgcgcctgtg ggcctataaa gcgatccggc agggctggcc
      421 ggattacccc gaatggttgg ccgcttgtgt tgaccgggca tcaggatata acgcccagtt
      481 cgagcagccg ctccccgcaa acgaagtccg gcacacggcc aagtccattg ccaaatggac
      541 gcaccagcac ctaagccccg cagggttcag agaggagcag gcccggagag gtgcgaaggg
      601 cggcaaggta tccaagggag ggggaaggcc gagcaatagc ggtaaggaca agtccgatct
      661 attgccggag gttctgcggc ttaaggctca ggggtacacc aaccgcgata tagcggacga
      721 tcttcaaatc tcccccagta cggtgagcgt ttacctaaag cgtgatcgtc cgtgatcgaa
      781 acaagcctta tcagataata gcccccttta ggggggttcc tgcgctcttg tcttttagcc
      841 ttaaaaaatc cttttagcgc ggatctttgg cgaggtggac aactgcgcct gtccgtccat
      901 cgcccccctg ttttttcggg gggtgtgggc ggcagcagtt atccacggag ccgctctttc
      961 ccttgatctt gctctagggc ttgcagtatc ccgcccatgg ttgaataatg tactaagcaa
     1021 ggctacacag aggcccctct acctgcttcg cggtctcggg cctctgtggc cttgttcagg
     1081 gtgctacgcc cccccgaaac cccctgtggc ccccgctcga tccgcccctt cgggacggtc
     1141 tgcgcggtgg cttcttggct ctggagaatg gacgaggccc tatgtcaaca acacagatag
     1201 agaaagattc gagaaggaag gcagtcaatg gcgttatttc tagtttccaa ctagaggggg
     1261 aggccataac cgatgaattc aaaattctac ttgaagatta tatcaatgga aaaatttcca
     1321 ccgatgaggt gttagctatg gtaagtgacg aacccacaac aacggaatac gactacgaaa
     1381 tcaccccccg cactctcggt cttggcgggg gctggaatct gcgtttgctg gagaacggcg
     1441 aagaggtcgg cgggggtgtt ttccccttgc cggagcactg cgattttcgg gatgaaaagg
     1501 cgctccagac gctactcgat agcctctatg aggacgcctt agcggaggcc tctgcatggc
     1561 tggccagccg ttaaagcgcc tccgctctat caagatccgg gtctcagatg cggagcttga
     1621 gcgcctgcgg gagctctgcc ccaaggccca actggcggag tggatgcggg agcaatgtct
     1681 cggtgttgcg caaccccagc gccgaacgcc ggcgcctacc gtagatccgg cgctcttgcg
     1741 gcagttggcc ggaatgggca acaacctgaa ccagatcgcc cggcgcgtta attccgggga
     1801 gtgggggccg ttggatcggc tgcgcatcat agcggagctg tccgctatag gccgcgagct
     1861 ggaggaactg caccatgatc atcaaattcc ataaccgtgg ggtaggccgg ggctccgggc
     1921 ctgttggcta cttgttgggc cgtgacggga accgggaagg cgcaacgctc gaccggggca
     1981 accctgcggt gattgaagcc ctgattgatt ccagtcccta cgccaaaaaa tacacctctg
     2041 gcgttctgtc gtttgccgag gctgatttac ccagggcgac caaggacaag ctgatgagcg
     2101 actttgaaaa ggcgctcctg cccggactgg agggcaacca gtacgctgtt ttgtgggtag
     2161 agcaccgcga caaggggcgg ctggagctga attttgtagt cccgaacatc gaattgcaga
     2221 gcgggaagcg gctgcaaccc tacttccaca aggccgacaa tccgcgcctc gatgcgtggc
     2281 gaaccgtggt aaacggggag ctgaaattgc acgacccgga cgatccgtta aaccgtcagg
     2341 ccgttatcac gccaaaggat ttgccgaggg ggcacaagga ggccgctcag gccattacag
     2401 acgggctttt acgcatggcc ggagcagggg agatccagag ccgcgatgat gtgcgcaaag
     2461 ccttgcaggg tgcgggtttc agcgtggtac gcgaaaccaa aaacagcctg agcattgccg
     2521 acccggacgg ggggcggaac atccgattaa agggggtgct gtatgagcaa gattttagac
     2581 atggcgaagg acttcgagca gagctcgaag cagcaggcca acgctatcga gaagcgtctg
     2641 accgaagaat tcacgaggca cgagaaagct atctccgggg cgttgaaatc aagcgaggag
     2701 aaaatgagcg ccgctattgc cgagcacagc cagaaaatgg gctggctgtt gctcaaaacg
     2761 tggatctgga tgctggtcag catcgtcctg ttcttgagcg caacgagcgg gatcctctgg
     2821 tatcaggggg aattgatagc ggagcggcaa gccactctcc aagccttggc agagaagggc
     2881 ggcaagctaa agctgaccca gtgcgggaag aaattggagc acctttgcgt gcagatctcc
     2941 gaccccaatc caaccgtttg ggggaaggat acctattgga tcgtgccgaa ggggtactaa
     3001 atgacgggat tggagcggga attgctctgc gcgttcgagg ctttgcagag cgcatacgag
     3061 cagcagcaca aggcatggca ggaaagctac gcgaacttgg cgagcatgta cgagagcacc
     3121 aaacaagagg ccgagatcag cagggcagca gcagagcgat tgacgaggca ggtgaacggc
     3181 ttaacgcagc aagtggacgg cttgagcaag cagctggagg gcttgaccgg gctagtcagc
     3241 aagttgcaaa agaaagggaa cacaggctcc tgaaggcact ggatcgaggc tgggagcgtg
     3301 gagacagatc gcgaggcttt gagcgttgac ggaacaaaac ggccgtcagt tataaaaaaa
     3361 ggaaagtgct atcactttcc ttttatcggc gggaattatc gttgagttct tctatgccat
     3421 ttttcatagt agtaactgta atcttcatct ctaccaccag attcaatcca gtcatcatac
     3481 tcagggctat ttttgaaatc tgaatcagac atgctaactc cttgttatta gttggcttct
     3541 atggaagcta gcgcaccgtg aagttatgat caacgatgta gtccacatca ttgtcgaaat
     3601 gtgcgttcag ctagtcttcg gtgttttctg ctttatttct agcggcgatt ctcgcgtctg
     3661 cccatgcggc agacttttta gcctgttctc gttgttcttc ggttgaagac tcgctgttga
     3721 gaactttgtt gctcgtttgt ttgaccaccg aatccaaacc tgttgttgct gcaaaatgag
     3781 ctaggttaac aactccctta cctaacaact tggcactagc gattgcggtt tctttagccc
     3841 caaaagcatc agacacctcc tcaagcgctt tagataggtt aaaaaatccc atactccccc
     3901 taagaatatc atcaataaaa ttgagtgttg ctctgtggat aacttgcaga gtttattaag
     3961 tatcattgca gcaaagatga aatcaatgat ttatcaaaaa tgattgaaag gtggttgtaa
     4021 ataatgttac aatgtgtgag aagcagtcta aattcttcgt gaaatagtga tttttgaagc
     4081 taataaaaaa cacacgtgga atttaggtaa tagttaaagc ttgtgcctga ttgttgccac
     4141 aattggtcag cagtttgcta gggaatgaga gggcagagaa tgataagctt agcacttaat
     4201 ttgtatggat atattgcatt aagcaagggg acgttatgcg tgtattagcc ttatcggctg
     4261 tgtttttggt ggcatcgatt atcggaatgc cagcggtagc aaaggaatgg caagaaaaca
     4321 aaagttggaa tgctcacttt actgaacata aatcacaggg cgtagttgtg ctctggaatg
     4381 agaataagca gcaaggattt accaataatc ttaaacgggc gaaccaagca tttttacccg
     4441 catctacctt taaaattccc aatagcttga tcgccctcga tttgggcgtg gttaaggatg
     4501 aacaccaagt ctttaagtgg gatggacaga cgcgtgatat cgccgcttgg aatcgtgacc
     4561 atgacttaat taccgcgatg aagtactcag ttgtgcctgt ttatcaagaa tttgcccgcc
     4621 aaattggtga ggcacgtatg agtaaaatgc tgcacgcctt cgattatggc aatgaggata
     4681 tctcgggcaa tgtagacagt ttttggctcg atggtggtat tcgcatttcg gctacccagc
     4741 aaatcgcttt tttacgcaag ctgtatcaca acaagctgca cgtttctgag cgtagtcagc
     4801 gcatcgtgaa acaagccatg ctgaccgaag ccaatggcga ctatattatt cgggctaaaa
     4861 cgggatactc gactagtatc gaacctaaga ttggctggtg ggttggttgg gttgaacttg
     4921 atgataatgt gtggtttttt gcgatgaata tggatatgcc cacatcggat ggtttagggc
     4981 tgcgccaagc catcacaaaa gaagtgctca aacaggagaa aattattccc tagaagtggt
     5041 tagcgcgtat ttgtgtgaaa tagccgtcat ataagctgta aagatatatg gacaaaatgc
     5101 tgatgctcga tgcgcttgta gttaatggct catacttaat cggtcaggcc taatcgactt
     5161 aagctccgcg cgttaacttt cagcctgagc attagcatca cattcctaaa cgtaaaaagc
     5221 ctagccaatt tgctaggctt ttttgttatc tcaatggcca tggtcactca acatgggggt
     5281 gtgaataaag gagtggcgat gtcagtaaag gtgaatgccg ctgcgataaa gtgcctagcc
     5341 catcaaggat tgtaggcttt cttggctgct aaagcgtttg acgaaaaagt ccaagaaggc
     5401 gctgatattg gttctccatc attttcatct accggtttac tataggtcaa ccaggccgtc
     5461 cttgccggcg cttgggacgc agacgatggc atcaaatgcc tcgctcaaat tcgtttctac
     5521 agaggcgctt tgggaccgca ttcgctttgc tttcatgggg gcatctgtca atgtcagaca
     5581 tgaatcctcc gatataggtt gatttttgat ctcttcaaca cttggtgttg tccgactaga
     5641 gagacttata tggatcagta ttcttgcact gacaatggtg ccactcaatc ccaaggatcg
     5701 acctagttag aggcgtagat caagcaattc aaaacagcgc agaagcagaa ggaggtggaa
     5761 atatgttcca gggtgaaagg ttacatgcaa ctaacgttgc tctctaggat gtgcctgctg
     5821 taacgtattg tccagaaaag acaaaccccc gttatcgctt gcatcttggc ggagctgcga
     5881 aaaacggggg ccgtgacgaa tcacaagaga agtctatcac atggatcatg ccaaacaacc
     5941 caacgccgcc aataagcgcg gtggtgcttg agaaagtgcg cacagcttga gctgtttcgg
     6001 gagcgcctgc cccggaaacc ctactattcc gacgagctta caacgggcct acggattgcg
     6061 gatgttgccc gcgcccttgg tgcgcgttac atccagccga acgggccaac gcaccgccac
     6121 tggatcgttt ttgacgtaga
//
