LOCUS       NZ_CP036196             9729 bp    DNA     linear   CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain BA34918 plasmid pColRNAI.
ACCESSION   NZ_CP036196
VERSION     NZ_CP036196.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10956502
            Assembly: GCF_004295405.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9729)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Narasimman,V. and
            Venkatesan,M.
  TITLE     BA34918 Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9729)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Narasimman,V. and
            Venkatesan,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2019) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method :: Canu v. 1.7; Unicycler hybrid v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 37.6x
            Sequencing Technology :: IonTorrent; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 23:31:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,183
            CDSs (total)                      :: 6,057
            Genes (coding)                    :: 5,729
            CDSs (with protein)               :: 5,729
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 328
            CDSs (without protein)            :: 328
            Pseudo Genes (ambiguous residues) :: 0 of 328
            Pseudo Genes (frameshifted)       :: 181 of 328
            Pseudo Genes (incomplete)         :: 155 of 328
            Pseudo Genes (internal stop)      :: 40 of 328
            Pseudo Genes (multiple problems)  :: 45 of 328
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036196.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9729
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BA34918"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pColRNAI"
                     /geo_loc_name="India"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2017"
                     /collected_by="Christian Medical College, Vellore, India"
     gene            <1..838
                     /locus_tag="EX353_RS32760"
     CDS             <1..838
                     /locus_tag="EX353_RS32760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004951794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_165394014.1"
                     /translation="KTKSAVKIARLGFATALDEEHERLYIGNTLDGSVTVIDTLTGKEL
                     SVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAADKGALWVIDMAVGKKIKTIDGLGH
                     AVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKKRFVVEPEKKHFFLNVSLDEKNGRA
                     FITDPDIPGVLVVDVNSGKILHRIDVINSLAILFNPLRNEIYITHRNAKQVSIVDSNSY
                     YVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEMSTMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            835..3024
                     /locus_tag="EX353_RS31350"
                     /old_locus_tag="EX353_31350"
     CDS             835..3024
                     /locus_tag="EX353_RS31350"
                     /old_locus_tag="EX353_31350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            4609..6570
                     /locus_tag="EX353_RS31370"
                     /old_locus_tag="EX353_31370"
     CDS             4609..6570
                     /locus_tag="EX353_RS31370"
                     /old_locus_tag="EX353_31370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            6570..7301
                     /gene="mobC"
                     /locus_tag="EX353_RS31375"
                     /old_locus_tag="EX353_31375"
     CDS             6570..7301
                     /gene="mobC"
                     /locus_tag="EX353_RS31375"
                     /old_locus_tag="EX353_31375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            7308..7838
                     /locus_tag="EX353_RS31380"
                     /old_locus_tag="EX353_31380"
     CDS             7308..7838
                     /locus_tag="EX353_RS31380"
                     /old_locus_tag="EX353_31380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7866..8045)
                     /locus_tag="EX353_RS31385"
                     /old_locus_tag="EX353_31385"
     CDS             complement(7866..8045)
                     /locus_tag="EX353_RS31385"
                     /old_locus_tag="EX353_31385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8114..8491
                     /locus_tag="EX353_RS31390"
                     /old_locus_tag="EX353_31390"
     CDS             8114..8491
                     /locus_tag="EX353_RS31390"
                     /old_locus_tag="EX353_31390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(8553..8852)
                     /locus_tag="EX353_RS31395"
                     /old_locus_tag="EX353_31395"
     CDS             complement(8553..8852)
                     /locus_tag="EX353_RS31395"
                     /old_locus_tag="EX353_31395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8842..9105)
                     /locus_tag="EX353_RS31400"
                     /old_locus_tag="EX353_31400"
     CDS             complement(8842..9105)
                     /locus_tag="EX353_RS31400"
                     /old_locus_tag="EX353_31400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            9482..>9729
                     /locus_tag="EX353_RS31405"
                     /old_locus_tag="EX353_31405"
                     /pseudo=""
     CDS             9482..>9729
                     /locus_tag="EX353_RS31405"
                     /old_locus_tag="EX353_31405"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /name="YncE family protein"
     Typing          complement(3229..3809)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6571..7298
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 gaaaacaaaa tctgccgtta aaatcgcacg tcttgggttt gccacagcac tggatgagga
       61 acatgagcga ctttatatcg gcaatactct ggacggctca gtgacggtca ttgatactct
      121 aaccggaaaa gaactttcag taattccgtt aactgaggga caggatgagg ctcatttcag
      181 accgaccaga gaaatggtca ttgacaaaaa aaatcagcgt ctttatgtct ccagcgccgc
      241 agataaaggg gctctgtggg ttattgacat ggctgtcggg aagaaaatta aaaccattga
      301 tggactgggg cacgctgtta ctggaattgc gttaaataaa aatggggata atatttatct
      361 ggttaacggg gatggtgaga ttattaacct ggatgccagt agctatgaaa taaaaaaacg
      421 gtttgttgta gagcctgaga aaaaacactt tttccttaat gtaagtctgg atgaaaaaaa
      481 cggacgtgca tttattaccg acccggatat tcccggggtg ctggttgttg atgtaaactc
      541 gggaaaaata ctacacagaa ttgatgttat aaattcactg gcgattttat ttaatccttt
      601 acggaacgaa atctatatca cgcaccgtaa tgcaaaacag gtcagcattg tggacagtaa
      661 ttcttattac gtaaaaacaa cgataaaaac ccgcctgatg ccaaacagtc tgtctttatc
      721 acatgacggt gaaaatctgt acgttagtgt aaaacaagaa aaaaaagaga tgagtactat
      781 gaaggattac atactcaaaa tcgaattatc taaaattgct atggagccag aagaatgaat
      841 aaatatttag ggctgagcat agtatatatg atgtatacat catctgttat ggctgatgat
      901 tctggcgcag ttagtaaaga taaaaatatt tcaccagaag aaacattaat tgttcatggg
      961 accgctgttt ctgaaagcac aacagaagga acagattcat acacaaccca gtcagccaat
     1021 acagcatcaa agatgaatct gtcattccgg gatacacctc agtcaactac agtcattacg
     1081 cgacgaagaa tggatgatgt agcagcaaaa aatattattg atgtggttaa aaccgctcct
     1141 ggattatatg tcagtacagc agaaggttcc ggaagaccga attttatgtc cagaggattt
     1201 aatgctaatg tcatgtatga aggatttagc ctgccgtgga attcatacat tccaagttca
     1261 caaactagtt cagctatttt tgaccgggtg gagatagtcc gcggggcaac aggtcttgtt
     1321 cagggtgccg gagacccttc agccgcgata aatatgatac acaagcgtcc gacccggcaa
     1381 tatcaggcca gcactaacat cggtgccgga cggtggaatg actaccgggg tggggttgat
     1441 gtatcaggac cactgaatga cagcgcgacc ttacgcggaa gggcagttgt ggatggcgaa
     1501 aacagtgaga ccttcaggga tagtgagaaa cgcaggaata acgtcattta cggaattatg
     1561 gaggcagatc tgactgataa taccctactt acgattgggg gatatcagca gtatgaatat
     1621 aacaatcatt ggtggtatga cttacctatt tctggtaccg gacaccacct taatcttcct
     1681 cgctcgacgt ttacaggcaa tgactgggag tttgaaagaa gtaaaagtaa aactgcattt
     1741 gcaactcttg agcagaagtt cagtaatagc tggaagctga aattttcaac gctgcaaaac
     1801 tggagaaatg ctgacctgtt agggacggca acataccggc ataaagatac ccgacaaagt
     1861 atgtatcagc aagtatgggg atcactgagg gaatataatt ccaaaagcta tgacctgtcg
     1921 cttaatgggg actatgcttt atttggtcgt gttcatcaat tcgtggctgg aacaactcag
     1981 caaaaataca ccgataaaga tacaggttat acatattccc caatatatat tgagaatgtt
     2041 aatatatttg atcatggttc acattcttcg aaaaaaccca tgaagatgtc aggtgatgca
     2101 tcctacgacg tcactgaaca aaaaagcgcc tacgcagcaa ctcattttct acttagtgat
     2161 aactggaaaa tattgctggg tggccgtctt gactggtatg aatatacaag taaaggtaca
     2221 tccattaata caggttataa agttaccaga aatatcacca aatatgcagg ttcaacatac
     2281 aatcttgacg atcatcatac catttatggg agctataccg atatattcaa tccacagtca
     2341 agcaccgacc tcaatggtaa agtgatcaag cctattgttg gtaagaatta tgagacaggt
     2401 attaagggtg agtattttaa cggcttgctt aatgcctctc tggcggtatt tcaggtagac
     2461 caggaaaatc gagccaggct gcttcccgat cagacagggt gtgttactaa tggcaatcct
     2521 caatgctatg aggctagcgg taaagtacgc acaaaaggga tagatttcga acttcagggc
     2581 atggttacgc cactctggga aattggggtg ggatatacat atgcagatac gcgatatata
     2641 aaagatggag atcgtagtaa tgaaggcaag cgattcaata ctgccgtgtc gccacaaaat
     2701 ttatttaaga tttcgagcat gtacaccttt gacggaaaat taacaaactg gcgagcagga
     2761 gtaaacacgt actggcaaag taaaatatat gctaacataa cagacacaag ggatggcaat
     2821 ccggttatta ataaacaaaa ggcttattca acaactgaca ttattattgg ctatactcca
     2881 atgccacaac tggatttaca gctcaatata actaatgtct ttgataaggt ttattataca
     2941 tccataggtg aatcgattgg atatggcacg gaaacatatg gagaaccaag aaatgttaaa
     3001 attagtgcaa cctatagatt ctaaccatgc ggattgggtc ttctcctcgg atttgcccct
     3061 ataaatccgt cttttgtggt tccggcgtac aaccccctgt ttgccacttc agttgatggt
     3121 taatcaaatc atgtagagga tcttcttgag atcctccatg gttcgtcgta atcttttgcc
     3181 ctgtaaacga aaaaccacct agggaggtgg tttgatcgaa ggttaagtca gttggggaac
     3241 tgcttaaccg tggtaactgg ctttcgcaga gcacagcaac caaatctgtc cttccagtgt
     3301 agccggactt tggcgcacac ttcaagagca accgcgtgtt tagctaaaca aatcctctgc
     3361 gaactcccag ttaccaatgg ctgctgccag tggcgttttt gcgtgtcttt ccgggttgga
     3421 ctcaagtgaa cagttaccgg ataaggcgca gcagtcgggc tgaacgggga gttcttgctt
     3481 acagcccagc ttggagcgaa cgacctacac cgagccgaga taccagtgtg tgagctatga
     3541 gaaagcgcca cacttcccgt aagggagaaa ggcggaacag gtatccggta aacggcaggg
     3601 tcggaacagg agagcgcaag agggagcgac ccgccggaaa cggtggggat ctttaagtcc
     3661 tgtcgggttt cgcccgtact gtcagattca tggttgagcc tcacggctcc cacagatgca
     3721 ccgaaaaagc gtctgtttat gtgaattctg gcaggagggc gaagcctatg gaaaaacgcc
     3781 accggcgcgg ccctgccgtt ttgcctcaca tgttagtccc ctgcttatcc acggaatctg
     3841 tgggtaactt tgcgagagtt agcagcgccc gccgcagtct cacggccgga gcgtagcgac
     3901 tgagtgaggg aggaggcgca atatcccccc tcccgcagac ggccaccgga cgcgcggatg
     3961 tgacatattg caccgtgtta ccgcctttcc cttccctgcc gataaatcat tcagataggt
     4021 gaagtctgtc ggttttctgc tgaatatttt acgttaacct atctctgttg cttttttctc
     4081 cttgcggaga aatgcaggtc atccccgctg gggcgtggct gacttcagga ggaaatcccc
     4141 tgcttacgct ggctggacgc caccgacgca cggtctttcc ccctctttct ggctcagctc
     4201 ggctggacgc cggccacgcc agaaattcac ccggcccccg tcaggctcac gcctgaccac
     4261 ccacaaaaac ccacggtgct gaacgcacct gacgaagcag caaagctgct cctgattttg
     4321 cgggtggtca gccgctcgct atggcgaact ctgacggggg ccggaacacc ggaaaagacc
     4381 gcactggggt gccgcctttt cctctcacac gctggggcgt gctcgttgtt cctccggggc
     4441 tgcgcctttt ccgcttgggg cggaaagagg ctggcctccg tggtgaaagc tgaatgcaat
     4501 caccacggac gtgaccgccg ccgccatgcc tcggggcatg tccgcggctt gtgcagcagg
     4561 ggctgcacac cacgccggta aacccgtcgt ctgaaggaga gagattgcat gtttaatacg
     4621 gattcgcttg cctggcagtg gcatatcccg gagctggccc ggcagattta cccgtgggtc
     4681 agctggctgt acagggagta ttacggtctg gccggggctt tcctggttat cggcctgttt
     4741 ttcggtctgg ctgaactctg ggccggacgg atgcgttttg tggggctgag tcataaggag
     4801 cgacgggagt atgccgatcg ctataagcgc gataataacc ggactgcgcc attttcggtg
     4861 ctggcttcgc tggattcgcc cgggcggatg gtcagggcac ggcggcatgt ctggaactac
     4921 gcgtactggc tgaccggcgc tgccggtctg gcccttctgg gggggctggt tgtcgccgtg
     4981 tttatcgaga gctggggaga aaccgtcctg cgtccggacc tctacgcgaa cgcagattat
     5041 tacctgctgc agccggctaa cctgttcggg attgtctggt gtgccggagg gcttgtcgcc
     5101 ggcatccttc tggcgcggct gctggcattt ctgctgcttg acggtcattt tgcggccgct
     5161 gacgaggcgg tgaatgcgaa actgaaccag gagagccggc gcagcagcca gcggaccgga
     5221 gaaatgaccg acgtccgcca cctgcatttc ggggagccgg taccggttaa cgcgctggcc
     5281 gatttttcca cagagcaggc ccggaaacag caggcggtgt ttctgggtaa ggatgagcag
     5341 ggccagccgg tgctggtccc gcgtgatacc tggcgcaaga ccaatattca gatcctcggc
     5401 ctgcccggga gcggcaagag cgtgatgggc accaacgcgc tcattcgctg cgtacgcgat
     5461 ttcggggacg cggtggtgta cttcgacccc aaaggggacg cgtgggcgcc gcacgtcttc
     5521 cgggcgcact gcccggactt caccctgctg gatctgcggc cggggaaacc ggcgcagctg
     5581 aatctgtttc gcgatctcga ccagtacgcc ctgaagaatc tgctggttgc cgggtttaac
     5641 ctgagtgaga ccggcgacgt ggcggaccac taccgcatca gcgagcagaa agcggcgaag
     5701 ctgattgcgg agcagttccc gcagggggca aacattcagc aggtgctggc ggcggcatac
     5761 gcgctgccgg aggccctgaa aaaggacgtg aaggggctga tcaccaagct ggagaacgtg
     5821 gccgacctga gcgtcctgca gacggacagc ggcatcgacg tggcggggat aatcaacggc
     5881 ggcggctgcc tgtacgtcat cggctcgatg gacgatgagg cggtgatccg ggtgcagaag
     5941 atgctgtttg cccgctgcgc gcaaatcatc attgcgcggg atgagttccg gaggtggccg
     6001 cacgcgagca tcatgctcga tgagattaaa tacctgctgt cgaagtacgt gctgaacgcg
     6061 ctggggacgc tgcgcagtcg ggactgcaac ctgctgctcg cgcaccagtc gctgggcgac
     6121 ttcgggcagt gcggccagga tctgcccgct gagtttgtga aaaccacggt gctggataac
     6181 acgccgatcc gctggttcta ccgggcggcc agccaggagt ccgcgcagtg ggcggcgggc
     6241 cagaccgggg aaatccgggt ggacgtggag cgccgccggg ccagccggga ggcggggaac
     6301 gtggagcata tcagcgggga cagcttcatt cagaaggagg cgcggccgct gtttgacgtg
     6361 aacaccctgc agcacctgcc tgacgggttt gcggtgatga ccgggctggg cgtggcccgg
     6421 atggcgttca gcagcccgct gcgggttgaa cgccgggaaa tcccgctgca gcgcttcccg
     6481 gtgctggcga aaacggaccc gctggcggag taccagccgg aggccgggcg gcagcggccg
     6541 ggtgatgatg attttgcggg gctgtactga tggcactgga gcgatacaac gtcagccacg
     6601 cgaagcggca ggcgcggaac gcggagaaaa cccggctgac cctgcgctgg ctgcgggaag
     6661 agctgtgttc cacggcggag ctggttgcgc gacggctggg gattgcggcg gtgcagccgg
     6721 tttaccgctt cctggacagc ctggtggcga aagggctgct ggtccgggcg aaatacccgg
     6781 ttgacggccg tcaggtcagc gtgtggggac tgacgccgca cggggtggcg ttctcgttcg
     6841 acgaggacga accgctgacg gacgttattc cgtttcagcc gtcaagggtg tcggcggcgc
     6901 agctgccgca ccggctggcc gtgcagtccc tgcgtctcgc catggaggcc cggggggcct
     6961 ccggctggcg gtacctgcac cgtatggccc tgaaggggat gaaggtgccg gacgcgctgg
     7021 cggagctgga cggcagaacg gtggcgtttg aggtggagcg caccgtgaag agccgcaggc
     7081 gctatcagga ggtggtgtcc gggtatctgt tcaatcgcaa agcgaacggc atcgatgaaa
     7141 tctggtacat ctgcccggac cgggcgacgc aggtcagggt gcagcgggcg atcctgtcgg
     7201 tggatgaaat cgtgaatcca cagaccggcg aggcccgcaa aacggcggag ctggaccggg
     7261 agcggctgtt tgcgtgcttt aaatttatga caacggagtg agggattatg gcgctgtaca
     7321 tggtatttat tgaagacgcg gaggaccggg acggcgagcg ctgcatcatg ccgaccagcg
     7381 agcatcttga agatatggtg gtgctcgcct ggcagaccgg ggcgtatgtt ttctgttttg
     7441 tcgaccgggc ccagcatctg gcgatgatga aatccatcgg gatgtatgac gagcggctca
     7501 acagcgacga aaaagccacg tactttttca ccggtaaaaa tccgcaatgg gaacgggaca
     7561 aacccgcagg aatgaagctg tgtgattacc tctatcgcaa ggtggagcgg ttcgccatcg
     7621 agaacggtgt gcaggtggtt gatgccggga agtgctcacc ggagcagatt agcgcgatga
     7681 ttgaggtggt cagcgagcgg cacggggaaa gcgcggtgaa cgagaagctg aaagcggagc
     7741 tgatgcggga atataacagc cgctatttta acgtgccggt gcctgaactg ccggaggaca
     7801 aacccgcccg ccggtggtgg ccgttcggca aaaaataaac ggcagcaccg gccatgccgg
     7861 tgctgttatt ccttcccgag cttatcccgc atcacctgcc agagtgtttc cgcatcctcg
     7921 tgcagccgct cgcacatgtc ggccggggta tccatatcca gctcgttcag tttctcgagt
     7981 aacagcagcg actggtcctt aataaattta gccatattga gggcagtttt ttcctgctta
     8041 gtcatgtttt ctgtccttcc gggtgtggtg tgcttcctgt cgggtatact gtctcaaaac
     8101 cggaggtgag acaatggcgt gggtgacggt gagacaggcg acggaactga cggggaaagc
     8161 ccggagttcc ctgtataggg atatggcgaa aggccgcgta tcataccgta ctgaagctga
     8221 tggtggtcgg gtggtggata ccagcgaact tatccgggtg tacggtgaat taagacagat
     8281 tgagactcac gagcgggacg ggttgagact gtcggatgag actgaaaaaa tggcctccga
     8341 cgcgttgatc gcggagataa aagcactcag ggaggaggtg gccggactgc ggcaggaaat
     8401 gcaggccatg agactgctgg aacacaaacc agagacagca gagacaccgg ccggaacatc
     8461 gcgccgatgg tggcagtggg gaaaacgctg acgcgctgtg cttgtccaac cggcgacggc
     8521 cacgcaatga attgcgcccc catcaccggt tatcagcgaa cggtatgggg attctggcgc
     8581 tggccaacca cggccagaac ccggactttc ctggtctggt catcaacatc atagaggaca
     8641 tgctgtccca tcagactgat ccggcgaacg tcctcgccat agcgtgagtc cacttcatat
     8701 aggcgcggga agtcggccag tttgtccgct gcagcgagaa ctcgatcggt aacgttctgc
     8761 gcatcccaca gggatgaatt ttccgcaatg tagtcaaaca atgctgtcac atcctcgatg
     8821 gctccctgct tccattccac ctcatacggc atcgcgtagt tcctgatagc ggccatgcag
     8881 tctctggcgg agttgttccg tggtcatgcc cgggcgcgtg tcagcgcggg cggcggtcac
     8941 tttagcgcga acctgatctt catattctgc ctgggccagt tcttcgttag ccaggcgctg
     9001 caacagtgca gctttgtccg ggccaccttc aggcagtatg cggggcagtc tctcggccag
     9061 ttcccgcagg aaattggcgt tttcatagtg tgtactgttg ctcattgtgg agcctcccgg
     9121 gctgatacgt cgatatgaag gagtttagca gggggcggtc tgaaagaata tgctcgtgtc
     9181 agtcaggctg atgaagacga aagctggtaa aaaagggctt acgacaccca ttttacgtca
     9241 aactgcacga catgcagttg cgtgcatagc catgcagcgg gttaaaattt atcaggcgcg
     9301 tttccggcgg ttttccgggt ggtttgttgc cggttttatc ccgtagccgc cggaaatgcc
     9361 ctcagaccgt ctgagcggtg cgcgtaatgc gacgttatgg taaatcatgt gattaatggt
     9421 agtgataatc attatcactt ttgctatatt cttgtttctt ttattgtaag gaaatgtaag
     9481 tatggcaatc acgattaaat ccgccgttaa tgtccgtagc agagtattaa tatactcttt
     9541 actactgttt actggttcag cgtatgcggc tgatatagct gaatcaacat tcctgactcg
     9601 tgatatcggc aatggtctgt atgaaatgtc ttttgatggc aaaaataata gtctctgggc
     9661 agcatcatcg tcttcattcg acagagataa gggccacgga attgtttatc agttagaacc
     9721 atccactct
//
