LOCUS       NZ_CP036185             2444 bp    DNA     circular CON 18-JUN-2024
DEFINITION  Escherichia coli strain WCHEC025970 plasmid p6_025970, complete
            sequence.
ACCESSION   NZ_CP036185
VERSION     NZ_CP036185.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10956393
            Assembly: GCF_004295365.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain WCHEC025970
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004295365.1-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 06:04:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,247
            CDSs (total)                      :: 5,127
            Genes (coding)                    :: 4,888
            CDSs (with protein)               :: 4,888
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 79 of 239
            Pseudo Genes (incomplete)         :: 160 of 239
            Pseudo Genes (internal stop)      :: 53 of 239
            Pseudo Genes (multiple problems)  :: 48 of 239
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036185.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WCHEC025970"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p6_025970"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2017-10"
     gene            join(1749..2444,1..273)
                     /gene="mobV"
                     /locus_tag="EYD11_RS26410"
                     /old_locus_tag="EYD11_26410"
     CDS             join(1749..2444,1..273)
                     /gene="mobV"
                     /locus_tag="EYD11_RS26410"
                     /old_locus_tag="EYD11_26410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            complement(343..573)
                     /locus_tag="EYD11_RS26415"
                     /old_locus_tag="EYD11_26415"
     CDS             complement(343..573)
                     /locus_tag="EYD11_RS26415"
                     /old_locus_tag="EYD11_26415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_124788667.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKHNYHLND"
                     /name="hypothetical protein"
     gene            complement(560..1042)
                     /locus_tag="EYD11_RS26420"
                     /old_locus_tag="EYD11_26420"
     CDS             complement(560..1042)
                     /locus_tag="EYD11_RS26420"
                     /old_locus_tag="EYD11_26420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223375251.1"
                     /translation="MNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     Typing          2..270
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.556"
                     /coverage="96.0"
                     /evalue="1.4000000000000002e-27"
ORIGIN
        1 gatggctttg caggaaccat cgctatggcc tctcagagcg cccagaacgc aaaaagagcg
       61 cgtgagctac agaagactat ggatgcgcag cagaaacgcc tacagagcgt cacagagcct
      121 tttaaggggc tttccagaga gcagatgacc gaaatcctga tgatggctca gcggtttaag
      181 cagcaaaacc aggaaaagga aaaacagcag cgtattgaac gtgaaaaaca gcgacagacg
      241 cgatcgcgtg gcatgggtgg tatggagcgt tagttttgat gcgatgcagg atagggctaa
      301 cgccgcaaag tgacgtcatt tgcggcattc accacgaaaa aattaatcat tcagatggta
      361 attatgtttt tttaaaatag aaaaagctgc gtttttctgc tttggtgaca aacccttagt
      421 tgagtattta ttaccgctag ttctacgata tatgctgtaa acactgctta aaaaagtacg
      481 ctcccaaaca gtcagaacct cattcttttt tagtgaatca tgcagcatta gcctgtctac
      541 atttttaaaa agtttatctt cagcattcat catccattac cttcgtctgc accgttcttc
      601 cattggtgtc ataactgatc tttaactcaa ttttctttct tctttcgtaa attgatggcc
      661 agtctccccg tgcaaaaaga aaggggttag cttcaagttc cccacgccct gcccggaaga
      721 atatttgttt atctacaaga gccgcaatta tgtttttaaa ggtctgatat tcaatatgaa
      781 ccctgggctg tatggttttt tcaaatctag tttttgatac agacacaaac ccatcccatc
      841 caatcagcat tgccaattgt aaaagcacat cattttgaga ttttgataaa ccatgaatca
      901 tcgcaatgtc tgcaatgtat agcttgacgt agggaggttc ggctgggatg gaaaaggtct
      961 cgatcttaga gtcttgtttt acctcaccgt tttcatccat gtatgtatgc cgttcaagcc
     1021 tagaaatctt tttcgtattc actccagccc cacgtttcgc catgatttag tactcgcatt
     1081 taagtactaa agagacatac agtcaagtcc tttgtatcaa gtacgattag tacttgaggt
     1141 gagtacgatt agtccttgag gtgagtacga ttagtacttg aggtgagtac gattggtact
     1201 tgaggtgagt acgattcttc gctctagccc ttggtacaag cgggttccca tatctatatc
     1261 tatatattaa gggggaaaat aagggctgaa aaacggcatt tttagacact gaaaaactgc
     1321 ctttttttag caaggagtgt acgcatttct atgtacgggg agtggaaatt ccgcttcctc
     1381 gctttggctg ttcgttcctc gtcgcgttgc tcctgcggta cgcctcagcc aaagctgcgg
     1441 cgagcggttc ctgctctttc aaagacgatt tcattgcctg atttcgtcaa gggcgagcgt
     1501 agcgagtgcc atttaccctt gacgacactc cggataaaac aggcttggca gtggtggggg
     1561 gtggcgcgaa gcgacgcctt ccacctgcca gaccgccgtt gattttgacc ttctggcagc
     1621 ttgctgcccg aacgattttg cgtagcaaag tctagtgagt ttacactatc atcttgatag
     1681 tggcccgccg gaggtatcgc aatgcgctcc cccgcctggc tgaatgaatg ccggaatcag
     1741 gggggaacat ggcgaactac gcgataatgc gctgcaaaaa gctgacggga atgggaagcg
     1801 tggccagcgc gttgcagcac tgctaccgag agcgtgagac gccgaacgct aacgctgaac
     1861 ggacaccaga aaactactgc tctgtgtcca aatcaacgga tcaggcaatg gggcgtgtgc
     1921 gggagctgct gccggaaaaa cgccgtaaag atgcggtact ggccgttgaa tacgttatga
     1981 cagcaagccc tgagtggtgg aaagaggcca caccacggca gcaggcggag tttttcgccc
     2041 gctctgagca atggctggag aagaaatacg ggaaggatcg tgttgtggcg gcggtggttc
     2101 accgggacga ggccaccccg cacctgtctg cgttcgtggt gccattgacg caggatggac
     2161 ggctgtccgc gaaagagttt attggcgggc ggtcaaagat gcgtgaagat cagagcacct
     2221 acgctgaatc agtaaaaaaa ctgggtcttg agcgcggcat cgagggaagc cgcgccacgc
     2281 atcagacagt tcagcactac tacgaatcga taaaccgtgg cacccgtagc caggtatcga
     2341 tctcgccgga ggcgctggag ccgcgtgtat tgcggaaggg cattttcacc aaagacgtgg
     2401 aagatcaggc agccatagcc aaacgtctaa gccacgcagt gaac
//
